LOCUS       DASRUP010000001         8203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1912742, whole genome shotgun sequence.
ACCESSION   DASRUP010000001 DASRUP010000000
VERSION     DASRUP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912742"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFE94_00005"
     CDS             complement(<1..457)
                     /locus_tag="VFE94_00005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZX49522.1"
                     /translation="MQESFLQPEMDREGGMIYSVEECHFLVQETLDRLGRVKVRGEMN
                     DVKTVRGQMAFFDLKDSNGKDFVVKCSIFGWEFKKYLHLIENGMEVVAVGKLRMYKNG
                     SLSIVVQEIQPYGEGAWRKAFEALKKKLEAKGYFDESRKREIPQVIQRIG"
     gene            complement(432..1292)
                     /locus_tag="VFE94_00010"
     CDS             complement(432..1292)
                     /locus_tag="VFE94_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZX49523.1"
                     /translation="MSRVLQKEIRWLVQEKYGGRRTPEAEKDIARLKKGEHVDYVIGW
                     VDFLGCKIDLSQQPLIPRPETEYWVGEVVEELRRGIFRNRAPYSAPYGAEPRPNPTIS
                     QNPSPPLPTGATARILDIFAGSGCVGVAVLKHVPWARVDFAEKDKNLLKQIRINARLN
                     GISSKRYRVLQSDVFSGVKGKYDYILANPPYVPESRKSRVQKSVLEQEPYHAVFGGKD
                     GLQYIRKFLREAKKHVVPGGAVYLEFDSLQKAEVVKLAKAAGPDGTVGVEPCKDQYNK
                     WRYARIVFTA"
     gene            complement(1311..2360)
                     /locus_tag="VFE94_00015"
     CDS             complement(1311..2360)
                     /locus_tag="VFE94_00015"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZX49524.1"
                     /translation="MPFAKKFLYIPLVVVGVLALLFIAFIVLVSLGGSRSSSSGGVSQ
                     GIPAGAPAPGGTVQFTNPLSSGGSVFDNLFAQRETSSVPSSNQPGSNNAQEGQLTQRK
                     ITKNGSLSLLVESAEDAAQRIQNIANDLQGFVSNSRVYEVSAGTKNGAVTIRVPSDKF
                     EEAVAAIKELASKVEQEQITSADVTEQFVDLEARLTNLRAEEAQYLEIMNRAGSISDT
                     LQVAQQLSRVRGQVEQLEGQLQFLARQVDMSTIQVSLTADSDVEVFGIRWRPLYIVKQ
                     EFRDMLEGLTWYINAMIAFVFWLPVLILWLGTSGIIFLVAWKLGRKVWRKFLKPQPTM
                     LIQDQPASPSRRKIV"
     gene            complement(2414..2635)
                     /locus_tag="VFE94_00020"
     CDS             complement(2414..2635)
                     /locus_tag="VFE94_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZX49525.1"
                     /translation="MAKIYVKAKPKAKKTEVRQIDSTHFEVAVVEVPERGLANRAILK
                     ALAEYLDMPVSRLSIVSGAAWRNKIVEIQ"
     gene            complement(2717..3472)
                     /locus_tag="VFE94_00025"
     CDS             complement(2717..3472)
                     /locus_tag="VFE94_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49526.1"
                     /translation="MSFYTAFKSEIKTLGIVLVFTILIIAVGIFTYFLSIPRVTVNDD
                     TITKPSSQPINTSAWQTYRNDEFGFEFQYSPEEWQLMEGDVQEDYGNIVWLFPRDSIG
                     ESAEPEIVIAIAHRPLEQEKEKREATSSDLEYNYSKTTIGGRTAYSSALLQGFHGLVR
                     EVLIDAGNDQTFIVSSNAIDELDQILSTFRFVEEQSDYVCRVPCTGAAPSSTLENTCW
                     KFTTQEQCTAFTVDESPYTCEWRPSSYQCPPLP"
     gene            complement(3509..4507)
                     /locus_tag="VFE94_00030"
     CDS             complement(3509..4507)
                     /locus_tag="VFE94_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZX49527.1"
                     /translation="MHEKKVFAVFEGGGVKGTGLVGAVEITEQQGYEFSHVAGTSAGA
                     IVASLVAAGYSSAEMKEIMMGLDYLKFKDEGFEDKIPLIGKPLSLLFTKGIYEGDYFE
                     AWIRDLLAKKNIRTFGDLVIEEYEECPRYRYRLQVVASDVSRGKLLVLPWDALDYGIH
                     PDELDVARAVRMSMSIPYFYKPIMLKDKQGHTSYMVDGGVLSNYPVWLFDPEGDNTTP
                     LWPTFGYKLVEPEEGKPRTIKGPISMLTALFATMMEAHDARYIKDANFLRTIPIETLG
                     IQTTQFDMTMEQKEALYESGKKAATEFFAHWSFEEYATKTLQTEHRTDKVWSHEKA"
     gene            complement(4514..5080)
                     /locus_tag="VFE94_00035"
     CDS             complement(4514..5080)
                     /locus_tag="VFE94_00035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZX49528.1"
                     /translation="MQKTIQLQKHRVAYTVRKHRRAKRMRVVVYCDGTVVVTVPRHMS
                     SAWAEQFLLENAHWVLEKLEQFRTAYVDLPRYTTKDYQQNKQRALQFVRQKVQEFNRW
                     YGYKVNNISVKNQKTRWGSCSGKGNLNFNYKILFLPEDVASYIIVHELCHLKEFNHSS
                     AFWGQVEQVLPNYRELRQELETRDFVLQ"
     gene            complement(5153..5782)
                     /locus_tag="VFE94_00040"
     CDS             complement(5153..5782)
                     /locus_tag="VFE94_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZX49529.1"
                     /translation="MIGFPRKIVLFDFDGVMVDSFEIGYQAAKKVGSPLNREQEKKLF
                     EGNVFEAVKKLNFKITPESQEAYFAEYVSGLMKLTPVAGMSETIQELAKAYQLVVVTS
                     SISSPIQGYLQMHHLGRYFASIMGSDVHQSKRTKIQMVFKEYQAGPENCVFVTDTLGD
                     MKEAAKCKVQSIGVTWGFHERERLQKGKFFAVVDKVEDLALAVHSFFGA"
     gene            complement(5779..6876)
                     /locus_tag="VFE94_00045"
     CDS             complement(5779..6876)
                     /locus_tag="VFE94_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZX49530.1"
                     /translation="MPIREPFAWLSWDDLEKLELLKAAFDNLADHVVITDTQGNVVYA
                     NKTVEVATGFSLEEVLGKNPGDLWGGNMPKEFYENMWHTIKEEKKPFVGEVQNKKKDG
                     TLYWQELHISPVLDNQNEVKFFIGIEPNITDRKQQEKFREEFVSILGHQLRNPLTTIT
                     WALEALFGYGSLNPHDRETLQEIYKQNRLLVDLVSDMLVVARFGNAKSEPVEFNLAEE
                     IRVIGARMSAANPQAKFHFETHEEQFYVRTNKSLALQVFQNLITNAFEYADKNSPRVA
                     LSVQQEKGVYVFSCENNGLSIPAEDQPKIFSKFFRASNATLAKERGTGLGLFIVKTIC
                     DYLGWRVSFQSPTEGGDGAIFFIQIPSLPEQ"
     gene            complement(6880..7650)
                     /locus_tag="VFE94_00050"
     CDS             complement(6880..7650)
                     /locus_tag="VFE94_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX49531.1"
                     /translation="MFELTNKVALVTGARRGMGKADALALAKQGAKIAVCDVELADCQ
                     AVVDEIKAQGGEAVAFQMDVSKAKEVNEVFDKVGEQFGRLDILVNNAGIYFSKPALEL
                     TEEDWDKMIDVNLKGQFLCAQRAAKEMTKNSPAGGGRIINIASIASGQVGVGIAGGAH
                     YTASKGGIIGMSETLAMEWAPLGITVNVIGPGAIDTPMVQAAQMPKEAMDALLQGIPM
                     KRMGRPEEVAAMVVFLASEEASYITGATLYVDGGWLAA"
     gene            complement(7659..8132)
                     /locus_tag="VFE94_00055"
     CDS             complement(7659..8132)
                     /locus_tag="VFE94_00055"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZX49532.1"
                     /translation="MNIRIIGALILGLVVIGGLGYLLAFWEGPKPEQEQDVTTVKLYY
                     YNAENDKDAEGNIQCSRQGLVSVEREVSQNETVIEDTIRLLLREELAAELPGVTLESA
                     ILENGVLTLEFADPQNKTVGGSCRVGILWFQIEATAKQFEGVEQVRFLPEELFQP"
BASE COUNT         1980 a         2225 c         1727 g         2271 t
ORIGIN      
        1 gcccgattcg ctggataacc tgcggaattt cgcgttttcg ggattcgtcg aaataccctt
       61 tggcctcgag tttctttttt aatgcctcga atgccttgcg ccacgcgcct tcgccatacg
      121 gctgaatttc ctgcaccaca atactcaaag aaccgttttt atacatgcgc aatttcccca
      181 ccgccacaac ctccatgccg ttttcaataa ggtgcaggta ctttttaaat tcccagccga
      241 atatcgagca ctttaccaca aagtctttgc cgtttgagtc tttgaggtcg aaaaaggcca
      301 tttgcccccg cacggtttta acgtcgttca tttcgcctcg cacctttacc ctgcccaaac
      361 gatcgagcgt ttcctgcacc agaaaatggc actcctccac ggagtatatc atgccccctt
      421 ccctgtccat ttcaggctgt aaaaacgatt cttgcataac gccacttatt gtattggtct
      481 ttgcatggtt ctaccccgac ggtaccgtcg gggccagccg ctttggctaa tttcaccacc
      541 tctgccttct ggagcgaatc gaattccaga tacactgccc cgccggggac aacatgcttt
      601 tttgcctcac gcagaaactt tcgtatatat tgtaacccat ctttgccgcc aaaaacagca
      661 tggtagggtt cctgctccaa cacggatttt tgcacgcgtg atttcctgct ttctggcacg
      721 tagggcggat ttgccaaaat ataatcgtat tttcccttca ctcccgagaa aacgtcggat
      781 tgcaaaactc gataccgctt tgaactgata ccgttgagtc tggcgtttat acgaatttgt
      841 ttgagcaagt ttttgtcttt ttcagcaaaa tccacccgcg cccaaggaac gtgctttaaa
      901 accgcaacgc ctacgcagcc agacccagcg aaaatgtcga ggattcttgc tgtagcgccg
      961 gtagggagcg gaggagaggg attttgagaa atcgtggggt tagggcgcgg ctctgcacca
     1021 tacggtgcag agtagggggc tcgatttcga aaaatccctc tccgaagctc ctccaccacc
     1081 tcccctaccc agtactctgt ttctggccga ggaataagcg gctgttgcga caagtcgatt
     1141 ttgcagccca aaaaatccac ccacccaatt acgtagtcca catgttcccc ctttttcagc
     1201 cgagcaatat ctttctccgc ttctggcgtt ctcctgcctc cgtacttttc ttgcaccagc
     1261 caccggattt ctttctgtaa aaccctgctc atttcgtatc aagaagtcca tcaaacaatc
     1321 ttccttcgag aagggcttgc tggctggtcc tgtattaaca ttgttggctg cggcttcaag
     1381 aatttacgcc aaacctttcg gcccaacttc cacgccacaa ggaaaataat tcccgacgta
     1441 cccaaccaca aaataagcac gggcagccaa aagacaaagg cgatcatggc gtttatgtac
     1501 caggttaaac cctcgagcat gtcgcgaaac tcctgcttta caatgtacaa aggccgccac
     1561 cgaatgccga acacttcaac atccgagtct gccgtaaggg aaacctgaat agtcgacata
     1621 tctacctggc gggccaaaaa ctgcagctgc ccctctaact gttcaacctg cccgcgcacg
     1681 cggcttagct gctgggctac ctgcaaggta tctgaaatac ttcctgcccg gttcataatt
     1741 tccaggtact gcgcctcttc cgcgcgcaag tttgtaaggc gcgcttcgag gtcaacaaat
     1801 tgttcggtaa cgtcggcgct ggttatttgc tcttgctcta ctttagaagc caactcctta
     1861 atggccgcta ctgcctcttc gaatttatct gaaggcactc gtatggtaac tgcgccgttt
     1921 tttgtgccgg ctgaaacttc atataccctt gaattgctta cgaaaccctg aagatcgttt
     1981 gcaatatttt gaatgcgctg ggccgcatct tccgcacttt ctaccagcaa agaaagtgaa
     2041 ccgtttttgg taattttgcg ctgggtaagc tggccttcct gcgcgttgtt cgaaccaggt
     2101 tgattcgaac tcggaacact ggatgtttcg cgctgggcga aaagattgtc gaacacgctc
     2161 cctcccgacg acaaaggatt ggtaaattgt actgttccgc ctggcgccgg agctccggcc
     2221 ggaattccct gcgacacccc gcccgacgaa gaagaacgcg accctcccaa actaaccagc
     2281 acaataaacg cgataaacaa caaagctaaa actcctacga ccaccaaagg aatatacaaa
     2341 aacttttttg caaaaggcat attcgtgtat tataccaact ccagtcaatc aagaaaggtt
     2401 gttatccaca ccgttactgt atttcaacaa ttttgttccg ccaagcggct cccgaaacta
     2461 tagaaaggcg cgatacgggc atgtccaaat attctgccag cgctttcaaa atcgccctgt
     2521 ttgccaaacc cctttctggc acctctacaa ccgccacctc aaaatgcgtg ctatcaattt
     2581 gcctcacctc cgtctttttt gcttttggct ttgcttttac ataaatcttc gccatatata
     2641 tccccatgtc cacttcgtag gtggacacgg gctacccacg cctttgcttt tacgcaaatt
     2701 ttaaagcagt gtacctttaa ggaagaggcg ggcactggta actgcttggc cgccactcgc
     2761 atgtatacgg ggactcgtcg accgtaaagg cagtgcactg ttcttgtgta gtaaacttcc
     2821 agcaggtatt ttccagcgtg cttgaaggcg cagcaccggt gcagggaacc cggcaaacgt
     2881 aatcggactg ctcctccaca aaccgaaacg tggacaggat ttggtcaagt tcatctattg
     2941 cgtttgaaga tacgataaaa gtttgatcat taccagcatc aattaaaacc tctcttacca
     3001 agccatgaaa tccttgcagc agggctgatg agtatgcagt tcttccgcca atagtggttt
     3061 ttgagtagtt gtattcaaga tcactactcg tcgcctcacg cttttctttc tcttgctcaa
     3121 gcggtctatg ggcaatagct ataacaatct ccggttcggc tgattcacct atactatctc
     3181 ttgggaataa ccacacaatg ttaccatagt cctcttgaac gtctccttcc ataagctgcc
     3241 attcttctgg tgaatactga aattcaaacc caaactcatc gttgcggtag gtttgccaag
     3301 cggatgtatt gattggttgt gacgaaggtt tagtaatggt gtcatcgtta actgtaacgc
     3361 gtggaatact aagaaagtaa gtaaagatgc ctacggcgat aatcagaata gtaaacacta
     3421 gtacaatgcc aagtgtttta atttccgact tgaatgccgt gtaaaaactc attgagttac
     3481 tataccattt tgtttatccc gttagaagct atgctttctc atgggaccag actttgtcgg
     3541 tgcggtgttc ggtttgcagg gtttttgtgg cgtattcttc aaagctccag tgggcgaaga
     3601 attccgtggc tgcttttttg ccgctctcgt acagcgcttc tttttgttcc atagtcatgt
     3661 caaactgcgt ggtttgaata ccgagcgtct cgattggtat ggtgcgcaaa aagttcgcgt
     3721 ctttaatata ccttgcgtcg tgggcttcca tcatggtggc aaataaagca gtgagcatgc
     3781 taatggggcc tttaatggtg cggggttttc cttcttccgg ctcaacgagt ttatatccaa
     3841 atgttggcca caatggcgtg gtattgtcgc cttcgggatc aaacagccac acagggtaat
     3901 tgcttaacac tcccccatct accatatacg aagtatgccc ttgcttgtct ttaagcataa
     3961 taggcttgta aaaatacgga atgctcatgc tcattcgaac ggccctagcc acgtcgagct
     4021 cgtctggatg gatgccatag tccaaagcgt cccacggcaa caccagcagc tttccccgcg
     4081 aaacatcaga agccactacc tgcaggcgat aacggtatcg cgggcactcc tcgtattctt
     4141 ctataaccaa atcgccgaat gttcggatat tcttttttgc cagcaggtcg cgtatccatg
     4201 cctcaaaata atcaccttcg tatattcctt tggtaaacaa taagctcaaa ggcttgccta
     4261 taagcggaat cttatcttca aagccctcgt ctttgaattt caagtaatcc aaacccatca
     4321 taatctcttt catttcggca gagctataac cggcagcaac caaagaagcc acaatggccc
     4381 cggccgaggt tcccgccacg tggctaaatt catacccttg ctgctcggta atttccacgg
     4441 ctccaacaag gccggttccc tttacccctc caccctcgaa taccgcaaaa actttctttt
     4501 cgtgcatatt ttcttattgt aacacaaagt cgcgagtctc gagttcctgg cgcagttccc
     4561 ggtagttggg caaaacctgt tcaacttgac cccaaaacgc gctcgagtgg ttaaactctt
     4621 ttaaatggca aagctcgtga acaataatat agctggccac atcttctggc aaaaaaagaa
     4681 ttttgtaatt aaaattaagg ttccccttgc cagagcagct cccccacctg gttttttggt
     4741 tctttacgct tatgttgttt actttgtacc cgtaccaccg attgaactcc tgcacttttt
     4801 ggcgcacaaa ttggagtgcc cgctgcttgt tttgctgata atcctttgtt gtatagcggg
     4861 gcaagtcaac atatgcggta cgaaactgct ctaacttttc caaaacccag tgtgcgtttt
     4921 ctaaaagaaa ttgttctgcc cacgcgctgc tcatatggcg cggcaccgta accactactg
     4981 ttccgtcgca atacacaaca acgcgcattc tttttgcgcg ccgatgtttt cgcaccgtgt
     5041 aggccacacg atgtttttga agctgaatgg tcttttgcat aaaggcttcc ttgctgcctc
     5101 cttattgccc ccattgtact ctttccgcag gaacgaaggc aagtataagc atttatgcgc
     5161 caaaaaacga atggactgcc agagccaagt cctcaacctt atccaccacg gcaaaaaact
     5221 tgcctttttg caggcgttcc cgttcgtgaa acccccatgt tacgccaata gactgcacct
     5281 tgcactttgc ggcttctttc atgtcgccta aggtatcggt aacaaacacg cagttttcgg
     5341 gccctgcctg gtattcttta aacaccattt gtatttttgt gcgtttgctt tgatgcacat
     5401 cgctccccat aatgctggca aagtaccgcc ccaaatgatg catttgcaaa tatccctgaa
     5461 tcgggctcga tatagacgaa gttaccacca caagctggta tgcctttgcg agttcttgta
     5521 tcgtttctga catgccggcc accggagtaa gtttcataag ccccgatacg tattctgcaa
     5581 aatacgcctc ctggctttcc ggcgtgattt tgaaattcag cttttttacc gcttcaaaca
     5641 cattcccttc aaacagcttt ttctcctgtt ccctgtttaa gggggagcct acttttttgg
     5701 ccgcctgata gccaatttca aaagagtcca ccatcacgcc gtcgaaatca aacaatacga
     5761 tctttctcgg gaaaccgatc attgttctgg caaagaagga atttgaataa aaaagatggc
     5821 gccgtcgccc ccttctgttg gactttgaaa cgacacccgc cacccaaggt aatcgcatat
     5881 tgtttttaca ataaacaggc caaggccggt tcctctttct tttgccaggg ttgcgttgga
     5941 cgcgcgaaaa aacttggaaa aaatctttgg ctggtcttct gcgggaatcg aaagcccatt
     6001 gttttcacag gaaaaaacat acacaccctt ctcctgttgt acggagagcg caactcgcgg
     6061 ggagtttttg tctgcatatt caaacgcatt ggtaataagg ttctgaaata cctgcaaggc
     6121 aagtgatttg tttgtcctta cataaaattg ttcctcatgc gtttcaaagt gaaatttcgc
     6181 ttgggggttt gccgcgctca ttcttgcccc tattacccta atttcttctg ccaggttgaa
     6241 ctcaacgggt tcagatttcg cgttcccaaa acgcgcgaca acaagcatat cgcttaccaa
     6301 atcaacaagc agccggttct gtttgtatat ttcctgcagg gtttcgcgat cgtggggatt
     6361 aaggcttccg tagccaaaaa gcgcttcgag cgcccaggtt atggttgtca aagggtttcg
     6421 caactgatgg ccaagaatag acacaaattc ttcgcgaaat ttttcctgct gcttgcggtc
     6481 ggtaatgttg ggctcaatgc cgataaagaa ttttacctcg ttttggttgt cgaggaccgg
     6541 agaaatatgc agttcctgcc aataaagagt cccgtctttc tttttgtttt gcacctcccc
     6601 cacaaaaggc tttttctcct cttttatggt atgccacatg ttctcataaa attccttggg
     6661 catattcccg ccccacagat cgccggggtt ttttcctagt acctcctcga gagagaaccc
     6721 cgtagctacc tctactgttt tgtttgcata caccacgttt ccttgtgtat cggttatcac
     6781 cacgtgatct gcgagattgt caaaagccgc tttcagcagc tccaactttt caaggtcgtc
     6841 ccacgatagc caggcaaatg gttctcgaat aggcatatgt taggcggcaa gccagccccc
     6901 gtctacgtag agggtggcgc cggtaatata acttgcttcc tctgaagcta aaaatacgac
     6961 cattgcggcc acctcttccg gcctgcccat tcttttcatc gggataccct ggagcaaagc
     7021 gtccattgct tctttgggca tttgagcggc ctgcaccata ggcgtatcaa tagctccggg
     7081 gccaattacg tttacggtaa tgcccaacgg cgcccattcc atagcaagag tttctgacat
     7141 tccaataatg ccgcccttgg aagccgtata gtgggcacca ccggcgatac ccacgcccac
     7201 ctgtcctgac gcaatcgacg caatgttgat aatccgacct ccgccagccg gcgaattttt
     7261 cgtcatctcc tttgctgccc gttgcgcgca aagaaactgt ccctttaggt taacatcaat
     7321 cattttatcc cagtcttctt cggtaagttc taaagcaggc ttggagaagt atatgcccgc
     7381 gttgtttact aagatatcca gacgtccaaa ttgctctccc accttgtcga atacttcgtt
     7441 tacttctttt gcctttgaca catccatttg gaaggctaca gcttcaccgc cctgggcttt
     7501 gatttcgtct accacggctt ggcaatcggc aagctcaaca tcgcaaaccg cgatttttgc
     7561 tccctgcttt gccaacgcca aagcatcggc ttttcccatg ccgcgtcgag ctccggtgac
     7621 gagtgcaact ttgttggtaa gttcaaacat acaaaagctt atggctggaa gagttcttcg
     7681 ggcaaaaaac gcacctgttc gactccttcg aattgctttg ccgtggcctc aatctggaac
     7741 cacaaaatgc ccacgcggca agaccccccc acggttttat tctgggggtc ggcaaactca
     7801 agggtaagca cgccattttc cagtatggca ctttccaacg taactcccgg caattctgcg
     7861 gcaagttcct ctctcaagag caaccggatg gtatcttcaa taactgtttc gttttgagaa
     7921 acctcacgct caaccgaaac cagtccctgc ctcgagcatt ggatattacc ctccgcgtct
     7981 ttgtcgtttt ctgcgttgta gtaataaagc tttaccgtgg tgacatcttg ctcctgctct
     8041 ggcttcggcc cttcccagaa cgcgagaaga taccccaacc cgccgattac cacaagcccc
     8101 aaaataagtg caccaataat acgaatattc atactgatag tataaaactt ataagttcag
     8161 ttgccaaatg gcgtagttga gatatgcggc aaacgcaacc cat
//
LOCUS       DASRUP010000002        13326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1273768, whole genome shotgun sequence.
ACCESSION   DASRUP010000002 DASRUP010000000
VERSION     DASRUP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13326
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273768"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFE94_00060"
     CDS             complement(<1..279)
                     /locus_tag="VFE94_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49533.1"
                     /translation="MSKKILFHFRKVDPVLYQAMRQLKPLAEIEPRKPAEYFSALCSD
                     IIGQQLSNKAAIPIEQRFIALFPRKRVSAKHLLNLSEEKIRGAGTSWAK"
     gene            complement(284..700)
                     /locus_tag="VFE94_00065"
     CDS             complement(284..700)
                     /locus_tag="VFE94_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49534.1"
                     /translation="MKKVIICGSISAADDILAAKKTLEEKGYEVEIPEGVKHLADWEG
                     SQASLEEKAERKIKYDLIRGYYEKMKGYDMVLVVNPEKKGVPGYIGGNTFLEMGFAHI
                     LNKPLYVLHEIPELSYASEILGMQPVMLNGDLEKLR"
     gene            complement(702..1124)
                     /locus_tag="VFE94_00070"
     CDS             complement(702..1124)
                     /locus_tag="VFE94_00070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HZX49535.1"
                     /translation="MNQECPFCTIAREDRILKEGEYMYVILSNPRLMPGHLLVIPKRH
                     VTRISEMTGEERKELLDLLIEFEEKILQKLATGCDIRQNYKPYVQNGKTSVKHFHFHI
                     YPRDLNDEMHQTIDPYQKPLYRDLPPEEKEKMFNLLSK"
     gene            complement(1121..1597)
                     /locus_tag="VFE94_00075"
     CDS             complement(1121..1597)
                     /locus_tag="VFE94_00075"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZX49536.1"
                     /translation="MEKFDWKKYIGECLESTKYCALATNDPEGVWVNPVYFAYDSSYN
                     FYFISQLESRHMKNIAKNAQVSVGIYSTQQTGGDVVGIQLIGEAHALGNEADIQKAYE
                     VYYGRKNPETKRDLEKSESDFMGSSAWHLVQIVPAKMYYFDTRFFEEVRQQVPLPL"
     gene            complement(1607..1933)
                     /locus_tag="VFE94_00080"
     CDS             complement(1607..1933)
                     /locus_tag="VFE94_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqfO family protein"
                     /protein_id="HZX49537.1"
                     /translation="MTQSNNVKIVVFVPVTHADKVRDAVGKAGAGKIGNYTFCSFSSK
                     GIGRFRPEQGANPTIGKVGKLELVEEERIEFVCSRAILQGVLKAMKEAHPYEEVAFDI
                     YPLEDI"
     gene            complement(1936..2268)
                     /locus_tag="VFE94_00085"
     CDS             complement(1936..2268)
                     /locus_tag="VFE94_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008027784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HZX49538.1"
                     /translation="MIAYGDFEKVDIRVGKVLEVKDFPEGKYSTHILKIDFGPEIGQK
                     TSLAKLAPNYQGQELVGRQVLGVVNFPPKQIGKYMSEVLTLGLADENNNVVLVHPGKD
                     VPFGGKLY"
     gene            complement(2271..2693)
                     /locus_tag="VFE94_00090"
     CDS             complement(2271..2693)
                     /locus_tag="VFE94_00090"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZX49539.1"
                     /translation="MENKTQKIVAIALIRNEEGKILVQKRVDPLHLEANGKWEFPGGI
                     VEFGETLAQAAEREAEEETGCSIEIVRLLPYAHMRIWERTDGTKVQVFVSCFEARLVE
                     GVARPLEKESSEVRWCTKEEIAALDTLPATNEFINLIE"
     gene            complement(2697..5213)
                     /gene="secA"
                     /locus_tag="VFE94_00095"
     CDS             complement(2697..5213)
                     /gene="secA"
                     /locus_tag="VFE94_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZX49540.1"
                     /translation="MRFFKELLGNPKGKEFKGGAKEVSQVNALEQEFEKLNAKELLGK
                     TTLLKERIQKGASLEELMPEAFATAREVSKRTLGQRHFDVQIMGGMVLHQGNIAEMRT
                     GEGKTLVATLPAYLNALGGKGVHIVTVNDYLARRDAVWMGQIYHALGLEVACITHQAS
                     YVYDPSYKEDAKARDEKRDEMGSFLVEESFLRPVSRKEAYATDITYGTNNEFGFDYLR
                     DNMAYSVEQQVQRGHHFAIIDEVDSILIDEARTPLIISAPDQESSTWYKDFAKITPQL
                     KKEFDYEIDEKLKAVSITDVGIDKVEKILGAKDIYQEKGIKYIHHLESALRAQVLFQR
                     DRDYVVRNNQIIIVDEFTGRLLPGRRYSGGLHQALEAKEGVQVQPESLTLASITFQNY
                     FRMYEKLAGMTGTAQTSAEEFYKVYGLEVVPIPTNKPNQRKDLPDRIYKSEQGKFLAV
                     VKEVAERHEKGQPVLVGTVSIQKNEFLGQLLQKEGISHQMLNAKNHEQEAAIIAQAGR
                     RGAVTVATNMAGRGVDIVLGGNPPDEEGAKEVRELGGLYVIGTERHEARRIDNQLRGR
                     SGRQGDPGSTQFFVSLEDDVMRIFGGGRIKGLMEKLHMPEDQPIENGMVSKSIESAQS
                     KIEGFHFDARKHVLQYDDVLNKHRDAMYRRRHEILEKAEKGELTEYILAMVQRQGNSK
                     EEYEKKEQELGPENMRQAEKLVALRTIDSLWLGHLEEMDYLRDSVRLRAYGQQDPLIE
                     YKNEGHRMFEQLLGAIEEQTAKTIMQVQLAPPPPQNPMGVSNVTYSSPANSTSNSAPQ
                     PRAQQARPAQPKIGRNDPCPCGSGLKWKRCGMISAPEHKA"
     gene            complement(5500..6435)
                     /gene="murB"
                     /locus_tag="VFE94_00100"
     CDS             complement(5500..6435)
                     /gene="murB"
                     /locus_tag="VFE94_00100"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZX49541.1"
                     /translation="MKQQLSGAQENVPLKGYTTFRIGGPARYFFVAKTAKDIENAMNA
                     ARKLKIPFFVLAGGSNTLIADKGFAGLVIKIQNSKFEFRNSSLWVEAGVPVSRIVRET
                     EKRGLSGFEWAGGLPGTVGGGVRGNAGAFGGEIKDRVESVEALDAKGKRRVFSAKQCR
                     FGYRNSIFKKRNYVVLAATFRLEKGDAKEIRAIAKSHVTYRKERHPLEYPNAGSIFKN
                     PKLKEVPQTLRNDFHEVIKNDPFPVIPAAAFLARAKLQGLRVGGAEISRKHPNYIVNR
                     QNASAKDVLLLIAKTKKQIQKKFFVLLEEEITFPY"
     gene            complement(6484..6861)
                     /locus_tag="VFE94_00105"
                     /pseudo
     CDS             complement(6484..6861)
                     /locus_tag="VFE94_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            complement(6872..7315)
                     /locus_tag="VFE94_00110"
     CDS             complement(6872..7315)
                     /locus_tag="VFE94_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VII domain-containing protein"
                     /protein_id="HZX49542.1"
                     /translation="MVKKCTRCGKTNSDVSFSLKKNSTSVIAKRCDDCRSSAAVTWGA
                     RNPEKIRVANERFKASGKARYSRLLKKYNISESLYLELLKKQGGRCAICKHSSPRGRY
                     PVFNVDHDHNTGRIRGLLCGNCNRGLGYLGENLKVLKSAINYVKK"
     gene            complement(7656..9398)
                     /locus_tag="VFE94_00115"
     CDS             complement(7656..9398)
                     /locus_tag="VFE94_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZX49543.1"
                     /translation="MRYTFAVIAFIGLCVLGAPLGAAADSLEQQVAFTVNPAFDAKER
                     DTLLASLRKITDQLYFYVEDEWWGLLTTAERQRALSNLDELALRFENEIYPGLTSRFG
                     PEERPGIDNDLRITILIHRMKGGLEGGYVNRADFYTKFESPKSNEREMLYLGAQEISS
                     PLAKSYLAHELTHVLYFSQKKAQRELQGDTWFEEGLAEVASTLLGYDNPGENSYSALR
                     LEQFVRDPQNPLAEWQNLLADYGVAHVFFQYVKDQYGPEFLAELMHSHQTGIAAFNDV
                     LAKRQRTETFHDIFTNWSIAVFLNDCSYGTQYCFSNPNFKNLRVVPRTNFLSPFGESQ
                     LTVSLQTKNWAGNWHRISGGKGELQIVFDGNPGVRFVVPYLIQGQNNGYSLGFFDLSS
                     EQLGTLRVPEFGDKHTALVILPSIQQQTSGFENSPSSFFSWTVSTTSENDVTNTDQAS
                     LLQQIAQLQAQVQALQEELRLLRGGGENQTACSFNENLSYGTRNQNGVKCLQEFLSRQ
                     GLDVYPEGLVTGNFLSLTREAVIRFQEKHATDILAPYGLNKGTGLVGQTTRKKINELL
                     QSQDIILTISQGGV"
     gene            complement(9422..11296)
                     /locus_tag="VFE94_00120"
     CDS             complement(9422..11296)
                     /locus_tag="VFE94_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZX49544.1"
                     /translation="MTFPKFQRTTRTRFAFFVAADTLLIAFSVVAAFFLRFEGSIPAS
                     YWSGAIQTTILLAVVFLLPLLYFFHLYWLSWAYVSTEELISLGKAVTLGFALLAGIVL
                     FLRDTELFQEFPRSTLLMSYVFAFFSIGALRMAKRIYMQVVENQETENGKRTLIVGAG
                     DAGEQLVRSLAAGRQNVRPVAFVDDNPIKKGVVIHGIKVVGTSNDIPRVVAQYQIEEM
                     IIALPSAGAQAIKRAVEKGREAGLRNMRVVPTLQDIVRKGVPVDALREVQVEDLLGRE
                     PVHLHAELIERFIAGKTVLITGAAGSIGSELCRQVARFHPEALVLLDQDETGIFQMLH
                     ELRRSFPNLQVRPVVGNIQDKSRVEYVFENFRPQLVFHAAAYKHVPLMEENISEAVKN
                     NIFGTLVVAKAADKYGAEKFVFVSTDKTVNPSSVMGATKRVGEMICQFENSRSDTKFI
                     SVRFGNVLNSRGSVIPLFRERIKRREALEVTHPEMKRYFMITSEACLLVMEAGAMGEG
                     GEVFVLDMGQPVKIVDLAYEMIRLSGLEPDRDIPVVFVGPRPGEKLFEEVLSAEEGTM
                     ETQHAQIHKARLSSVNREMLEQGLEQLLSAVDSPQKEQCRLILQELVPTYTPQLWNKS
                     "
     gene            complement(11283..12401)
                     /locus_tag="VFE94_00125"
     CDS             complement(11283..12401)
                     /locus_tag="VFE94_00125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZX49545.1"
                     /translation="MIPLSAPDITEAERKAVQEVLETATLSLGPKQKEFEEKVAQYVG
                     RKYAIAVNSGTAALHLIVRALGIGEGDEVITTPFSFISSSNCILFENATPVFCDIDEQ
                     TLQIDPAKIEAAITQKTKAILAVDVFGHSADWDAILQIAEKHKLFVIEDSAEAIGGLY
                     KGRKCGSFGNAAIFSFYPNKQITTGEGGMILVDDNITAELCQSMANQGRKVKGGEWLE
                     HVRLGYNYRLGEIPCALGVAQLGRLQEILQKRDRVANQYTEKLQELSGVQVPWVSPDV
                     MMSWFVYVIRLEKSYTKEQRDAVLAYLENKGVQCRPYFVPIHLQPFYRQKGYTEGDFP
                     VTESVGNRTIALPFFNNLSEQDIDTVVTTLQHALHDVS"
     gene            complement(12398..12790)
                     /locus_tag="VFE94_00130"
     CDS             complement(12398..12790)
                     /locus_tag="VFE94_00130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZX49546.1"
                     /translation="MKRFSGWKPPKIEEGKLTKYNWMVQHAKNLRLGKYTDIGAFCYI
                     NAKNGIVIEDNVQIGSHCSIYSVSTIDEKEGKVVLKKNCKIGAHSVVMPGVTVGENSV
                     VGALSFVTKDIPANVVAFGVPAKVVKKL"
     gene            complement(12792..>13326)
                     /locus_tag="VFE94_00135"
     CDS             complement(12792..>13326)
                     /locus_tag="VFE94_00135"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZX49547.1"
                     /translation="IPFVLIAGAIKLDSKGPVFFIQERIGKDGKPFRMMKFRSMVDNA
                     IQLGKGIEVEQNDTRITRVGKFLRRFTLDESPQIVHILFGTMSFVGPRPALLHQVAQY
                     STRERRRLEVKPGMANMAMLKGWNRLSWKERIEWDIWYIEHWSLWLDIKIVLLTAKAV
                     FSGQGQYGANGVVEDYK"
BASE COUNT         3209 a         3502 c         2771 g         3844 t
ORIGIN      
        1 ttttgcccat gaagtgcccg cgccccgtat cttctcctcg gacaagttca acagatgctt
       61 cgcgctcact ctctttcgcg ggaacaaggc aataaatctt tgctcaatgg gaatcgcagc
      121 tttgttggaa agctgttggc caataatatc agaacacaga gcagaaaaat actccgccgg
      181 ctttctcggc tcaatttcgg ccaacggctt tagttggcgc atggcctggt acaacacggg
      241 gtctactttg cgaaaatgga acaggatttt tttcgacatc tctttatcgc aacttttcca
      301 aatcgccgtt tagcattaca ggttgcatgc ccaaaatttc agaagcgtac gacagttcgg
      361 gaatttcgtg caacacgtat aagggcttgt ttaaaatatg ggcaaacccc atttccaaaa
      421 acgtgttgcc cccgatatac cctggcaccc ctttcttctc cgggtttacc acaagtacca
      481 tgtcgtagcc tttcattttt tcgtagtagc cgcgaattaa gtcatacttt attttgcgct
      541 ctgctttctc ttctaaagag gcttggctgc cttcccagtc tgctaaatgc tttacccctt
      601 ctggaatttc cacctcgtac cctttttctt ccagcgtttt cttggcagct aaaatatcat
      661 ctgccgcgct tatggacccg caaataataa cttttttcat gttacttgct taacaggtta
      721 aacatttttt ctttttcttc gggcggaagg tcccggtaca aaggcttttg gtacggatct
      781 atagtttggt gcatctcgtc gtttaggtcg cgcggataga tatgaaagtg aaaatgcttt
      841 acgctggttt tgccgttttg cacataaggc ttgtagtttt gccgaatgtc gcagcccgtg
      901 gccagctttt gcaaaatttt ctcttcaaac tcaataagca aatctagaag ctctttcctt
      961 tcctcacccg tcatttctga aatccgcgta acgtgccgct ttggaataac caacaaatgc
     1021 ccgggcatta acctggggtt gctcaaaatc acatacatat actctccttc tttaagaatt
     1081 ctatcctcac gagctatggt acaaaatgga cattcttgat tcataaaggc aatggaactt
     1141 gctggcggac ttcttcaaaa aagcgcgtat cgaagtaata catttttgcg ggcacaattt
     1201 gcacaagatg ccatgcagag ctgcccataa aatcgctttc gcttttttcc agatctcttt
     1261 tggtttcggg attcttgcgg ccgtaataca cctcgtaagc cttttgtata tctgcttcgt
     1321 ttcccaaagc atgggcctcg cctatgagct gaatgcctac cacgtctccg cctgtttgct
     1381 gggtgctgta aattcccaca gaaacctgcg cgtttttcgc aatgtttttc atatgccgcg
     1441 actccagctg cgaaataaag taaaagttgt agctgctgtc gtaggcaaag tatacgggat
     1501 ttacccaaac cccttccggg tcgtttgttg ccaacgcgca gtatttggta gattccaaac
     1561 actcccctat atactttttc cagtcaaact tttccataac tcaaatttat atatcttcta
     1621 atgggtaaat gtcgaaggcg acttcttcgt atggatgggc ctccttcatg gcttttaaca
     1681 caccttgcag gattgcgcga gaacatacaa actctatacg ctcctcttca accagttcta
     1741 acttacccac tttgcctatt gtggggtttg ctccctgttc tgggcggaac ctccctatcc
     1801 cttttgaaga aaagctacaa aaggtgtagt tgccgatttt cccggccccg gctttgccaa
     1861 ctgcatcgcg gactttatcg gcatgggtaa ccggcacaaa caccacaatt tttacattat
     1921 tcgattgggt catagttaat acagtttgcc gccgaagggc acgtctttgc ccgggtgtac
     1981 gagcactaca ttgttgtttt cgtcggctaa gccaagggta aggacttctg acatatattt
     2041 accgatttgc tttgggggaa agtttactac tccgagcact tgcctgccca ccagttcctg
     2101 cccctgataa tttggtgcaa gtttagccaa agaggttttt tggccaattt ctggaccaaa
     2161 gtcaattttt aaaatatgcg tggagtattt gccttcggga aaatctttta cttccagcac
     2221 tttccccacc cgtatatcca ctttttcaaa atctccgtat gcaatcatgc ttactcaatc
     2281 aagttgataa attcgttagt ggcgggcagt gtgtcgagtg ctgcgatttc ttctttggta
     2341 caccacctta cctcggaaga ctctttttcc aaaggccggg caacaccttc tacaaggcgc
     2401 gcctcaaaac acgaaacaaa aacctgcacc ttggtgccat cagtacgttc ccaaattcgc
     2461 atgtgggcat acggcaaaag ccgcacaatt tctatgctac acccggtttc ttcttctgcc
     2521 tcgcgctctg ctgcctgggc cagggtttcg ccaaattcca ctatgccacc cggaaattcc
     2581 cattttccgt ttgcttccaa atgcaaaggg tcaacccgtt tttgcaccaa aatttttcct
     2641 tcctcgttgc ggattaaagc tattgcaaca attttttgcg ttttattttc catattttat
     2701 gctttatgct cgggcgcact aatcattcca caacgcttcc acttcaaacc cgaaccgcag
     2761 gggcaggggt cgtttctacc gatttttggt tgtgctggcc ttgcctgttg cgctcgtggt
     2821 tgaggagcag agtttgaagt ggagtttgcc gggctggaat atgttacgtt gctgacgccc
     2881 ataggatttt gcggcggggg gggcgccaac tgcacctgca tgatggtttt ggcagtttgc
     2941 tcttcaattg ccccaagcaa ctgttcaaac attctgtgcc cttcgttttt gtattcgatt
     3001 aacgggtcct gctggccgta tgcccgcaag cgcacggaat ctcgcagata atccatttct
     3061 tccaagtggc ccagccacaa ggagtctatc gtacgcaaag caacaagttt ttctgcctgg
     3121 cgcatatttt ccggcccaag ctcctgttct ttcttttcgt attcttcttt ggaattgcct
     3181 tgtctttgca ccattgcaag aatgtattct gtaagctctc ctttttcagc cttttcaaga
     3241 atttcatggc gcctgcgata catggcatct ctgtgcttgt ttaacacgtc gtcgtattgc
     3301 agcacgtgtt ttctggcatc aaaatgaaac ccctcgattt tagactgggc agattcaatg
     3361 ctttttgaca ccatgccgtt ttcaataggc tggtcttcgg gcatgtgcag cttttccata
     3421 agccctttaa tccttccgcc gccaaaaatg cgcatgacgt cgtcttccaa gcttacaaaa
     3481 aactgcgtgg agccaggatc accctgccta cctgaacgcc cgcgcagctg gttgtcgatg
     3541 cgccgtgctt cgtggcgctc cgtgccaatc acgtacaaac ccccgagctc tcgcacctct
     3601 ttggctccct cctcgtctgg cggattaccc cccaagacaa tatccactcc gcggcctgcc
     3661 atattggtag ccacggtaac cgccccgcgg cgccccgctt gagcaataat ggcggcctcc
     3721 tgttcgtggt ttttggcgtt gagcatttgg tgcgaaatcc cctctttttg caataactga
     3781 cccaaaaatt catttttttg aatggaaact gtgcccacca ataccggctg ccctttctcg
     3841 tggcgctctg caacttcttt taccactgcc aaaaacttgc cttgctctga cttataaatg
     3901 cgatcgggca aatccttgcg ttggtttggc ttattggtag gaattggcac tacctccaaa
     3961 ccgtatactt tataaaactc ttctgcggag gtttgggcag ttcccgtcat gccggctaat
     4021 ttctcgtaca tgcgaaaata gttttgaaac gtaatagaag ccaatgttag ggactctggc
     4081 tgcacctgca caccttcttt tgcttccagg gcctggtgca aaccgccaga atatcttctc
     4141 ccaggaagta aacgcccggt aaactcgtcg acaataataa tctggttgtt gcgcactaca
     4201 tagtcgcggt cgcgctggaa aagcacttgc gcacgcaacg cgctttccaa gtgatgaata
     4261 tattttattc ccttttcctg gtaaatatcc ttggccccca aaatcttttc tactttgtcg
     4321 ataccaacat ccgtaatgga aaccgcttta agtttttcgt ctatttcgta gtcaaattcc
     4381 tttttgagtt ggggcgtaat ctttgcaaaa tctttatacc acgtggaact ctcctgatcg
     4441 ggagctgaaa taattaaagg cgttcgcgct tcgtcgatta gaatagagtc cacttcgtcg
     4501 ataatggcaa aatgatgacc tctttgcact tgctgctcta cactatacgc catgttgtcc
     4561 cgtaaatagt cgaacccgaa ctcgttgttt gttccatacg ttatgtctgt tgcgtatgct
     4621 tcttttctgg aaaccggccg caaaaatgat tcttctacca aaaaacttcc catttcatcg
     4681 cgtttttcgt ctcttgcttt ggcgtcttct ttatacgagg ggtcgtatac ataagaagcc
     4741 tggtgcgtaa tgcatgcaac ctcaagcccc agcgcatggt agatttgccc catccacacg
     4801 gcgtcgcgac gggccaggta atcgtttacc gtaacaatgt gcaccccttt ccctccgagc
     4861 gcatttaaat acgcaggcag ggtggccaca agtgttttcc cctctcccgt acgcatttct
     4921 gcaatattcc cctggtgcaa aaccatacct cccattattt gtacgtcaaa atgccgctgg
     4981 ccgagcgtgc gtttggaaac ctccctcgct gttgcaaaag cttccggcat gagttcttcc
     5041 agagaagcgc ctttttgtat gcgctctttg agtagcgttg tctttcccag caactctttc
     5101 gcgttcagct tttcgaattc ctgctctaat gcgtttacct gcgatacttc ttttgcgcca
     5161 cccttgaact ctttgccttt gggattaccg agtaactcct taaagaatct catagcaaga
     5221 atgtagtata ccagattatt cccgcaaaaa caaactcccg cctgaaaggc gggagttcag
     5281 agaaagaaaa gactatcttc tctttctgcg acgagtcgtc ttcttcgctg ccttcttggc
     5341 agtttttttc gaagaagctc tttttcttct tcttgctggc attggttttt ttgcaaatgc
     5401 tttgtattgt ttgtttgcaa aatagcacga ccttccaaaa cattacttac tatccacaaa
     5461 caaactatta gtatattttt tcacttgcaa gagctaatgt caatagggaa aagtaatttc
     5521 ttcttccagc agaacaaaaa acttcttttg tatttgtttt tttgtctttg caatcaacaa
     5581 aagcacatct ttcgccgatg cgttttggcg atttacaatg taattagggt gttttcgcga
     5641 aatttccgcg cctccaactc gtaatccctg gagttttgcc cttgctaaaa acgcggcggc
     5701 gggaatcacg ggaaaaggat cgttcttaat tacttcatga aagtcgttgc gcagggtttg
     5761 gggaacttct ttcagtttgg ggtttttaaa aatactgcct gcgttcggat actccaaagg
     5821 atgcctttct ttgcgatatg ttacgtggct ttttgcgatt gcccgaattt cctttgcatc
     5881 tcctttctct aaccgaaacg tggccgcaag caccacgtag tttcttttct tgaaaatcga
     5941 attgcgatag ccaaaccggc attgtttggc agaaaaaacc ctgcgtttcc cttttgcgtc
     6001 taaggcttcc acgctttcga cacggtcttt tatttcgccg ccaaaagctc ctgcgtttcc
     6061 ccgcacgccc ccgcctacgg tgccaggcaa accgcccgcc cattcaaaac cggaaagtcc
     6121 gcgtttttct gtttcgcgca ctatcctgga aaccggcacg ccagcttcaa cccacaagga
     6181 agaatttcga aattcgaatt ttgaattttg aatttttatt accaaaccgg caaaaccttt
     6241 gtctgcaata agggtgttgc tgccgccggc cagcacaaaa aagggaatct tcagtttcct
     6301 tgcggcgttc attgcattct cgatatcttt tgctgttttg gccacaaaaa aataccgggc
     6361 cggcccgcct atgcgaaacg tcgtataacc tttcaggggc acattctctt gcgccccgga
     6421 caattgttgc ttcaaagcat gaaatttatc catagaagtg acgacgaatc ttacaacgca
     6481 atgttacaga tttttcctcc ttaacttttc tggacttttt aaacctttct tttcttcaga
     6541 tttcaatttt cgctctactt ttttgatgtc ttcctctggc ggtaacgctt ccggacgaat
     6601 cccacgctcc aagagtgttc ttcgcacgct tgcgttattc gtactgtgtt cttcagatat
     6661 ttgatattcg gttatgagat ttttatcctt agaattaaat atagtgattt cggttgcaaa
     6721 atcttttgcc ttgagagtaa ttgttggtag aaaatcagct aatgccctac tcccagggac
     6781 tcggagccta tatttcattt cttgagttgt gtacccaaat agcgcccggt ctcccttact
     6841 tcttatcaac gcaaagtttt tatcgatact tttatttttt gacataattt attgcggatt
     6901 ttaaaacttt taagttttct ccaagatatc ctaatcctct attacaattc ccacatagca
     6961 atccacgaat acggccagta ttatgatcgt ggtcaacatt aaaaacagga taacgtcccc
     7021 taggagaaga atgcttgcat attgcacatc ttccaccttg tttcttcaaa agttccagat
     7081 aaagagattc acttatatta tactttttaa gaagacgaga atatcgtgct ttcccacttg
     7141 ctttgaatct ttcgttggct actcgtattt tttcaggatt tctagccccc cacgtaacag
     7201 cagcactact gcgacaatca tcacaccttt tagcaattac actagtacta tttttcttta
     7261 gcgaaaacga aacatcggaa ttagttttac cacaacgggt gcatttttta accataaaac
     7321 cattttacca caatccacaa tggttagtaa atactaccct ttcccgaaag cgatcgaata
     7381 atttcttttt tcatataccc catcatgccg taaaacggcc ttaaaatcaa ctactactat
     7441 aaacgaaaaa ccggcatttg tatgacaaaa gtccggcttt tcagtggatc ggaagcggta
     7501 actccagttg taagacttaa tgagtgctgc cttccgaccc aagtgtataa tacaggaact
     7561 ctctttacaa agcaaaaacc cccaaagggg gttcttgccg tcttacgtta gggagctgtg
     7621 tacagtaaaa aactgtaaca acactcatta ggtcattata ctcctccctg cgaaattgtc
     7681 aagatgatgt cttggctctg cagcagttcg ttgatttttt tcctcgttgt ttgccctacc
     7741 aggcccgtcc ccttgttcag gccgtagggc gccaggatat ctgtggcatg tttttcctgg
     7801 aacctgatta ccgcttcgcg cgttaaggaa agaaagtttc ccgtaaccaa gccttcgggg
     7861 tacacgtcta acccttgccg ggaaagaaat tcctgcaggc actttactcc gttttggttt
     7921 cgggttccat acgacagatt ttcattgaat gaacaggcag tctggttttc tcctccccct
     7981 cgcaaaagac gaagctcttc ttgcaacgcc tgaacctgcg cctgaagctg cgcaatctgt
     8041 tgtaataacg acgcctgatc ggtatttgtt acatcgtttt cggatgtggt agaaaccgtc
     8101 caggaaaaaa aggaggaggg gctgttttca aaccccgagg tctgctgttg tatggaaggg
     8161 agaataacaa gggctgtatg cttgtcgcca aactccggaa cccgaagcgt gccgagctgc
     8221 tcgctagaaa gatcaaagaa gccaagggaa taaccgttgt tttgcccctg aatgagatat
     8281 ggcaccacga agcgtacccc aggattcccg tcgaacacaa tctgcaactc acctttccct
     8341 cccgaaatcc ggtgccagtt gcctgcccag tttttcgttt gcaaagacac cgtaagctgg
     8401 ctttccccga agggagaaag aaagttggtg cgcgggacaa cgcgaagatt cttgaagttt
     8461 ggattggaaa aacagtattg ggttccgtac gaacaatcgt tcaggaacac cgcaatcgac
     8521 cagttcgtaa aaatgtcgtg aaacgtttct gttcgctggc gcttggccaa cacgtcgtta
     8581 aaagccgcga ttcccgtctg gtgagaatgc atgagttccg ccaaaaactc cggcccatac
     8641 tggtctttca cgtactggaa aaacacatgc gccactccat agtctgcgag cagattttgc
     8701 cattcggcaa gagggttctg cgggtcgcgg acaaactgct ccaaacgcaa ggcagaatag
     8761 gaattctccc cggggttgtc gtatccgaga agcgtggaag caacctctgc cagcccttct
     8821 tcaaaccacg tatctccctg gagctctcgc tgcgcctttt tttggctaaa gtacagcaca
     8881 tgggtgagtt cgtgcgcgag gtaacttttg gccagaggag aagaaatctc ctgcgcgccc
     8941 agatacagca tttcccgctc gttggacttg ggggattcga atttggtgta aaaatccgca
     9001 cgattcacat atcccccttc caaaccgccc ttcatgcgat ggatgagaat ggtaatgcga
     9061 aggtcgttgt cgatgccagg cctttcttcc gggccgaacc gggaagttag ccccggataa
     9121 atctcatttt caaaacgcaa agcaagctcg tctagattcg agagagcccg ctgcctttct
     9181 gccgtagtca acaaccccca ccactcgtct tcgacataaa aatagagttg atcggtgatt
     9241 ttgcgcaagg aagccaaaag agtatctctt tcttttgcgt cgaatgcggg gttgaccgta
     9301 aaagcaacct gctgttccaa ggaatcggcc gcggcgccaa ggggtgcccc caaaacgcaa
     9361 agccctatga aagcaatgac tgcgaatgta taacgcatat cacttgtatg gtagcaaatc
     9421 ttcaagactt attccacaat tgaggagtat aggtaggcac taactcctgg agaatcaaac
     9481 ggcattgttc tttttgcggg ctgtcaactg ctgaaagcag ctgttccagg ccttgttcga
     9541 gcatttctct gtttaccgag gaaagccttg ccttgtgaat ttgcgcatgc tgcgtttcca
     9601 tggttccctc ttctgcagat agcacttcct caaacagctt ttctcccggc ctcggcccga
     9661 cgaacacgac ggggatgtcg cgatctggtt ccagacccga caacctgatc atctcatacg
     9721 ccaaatccac aatctttaca ggctggccca tgtcgagcac aaacacctcg cctccttcgc
     9781 ccatcgcacc agcttccatt accagcaaac aagcctcgga cgtaatcata aaataacgtt
     9841 tcatttcagg atgcgtgacc tccaaagctt ccctgcgttt aatgcgttcg cgaaacaacg
     9901 ggatcacgct ccctctgctg tttaacacgt tcccgaacct gaccgaaatg aatttggtat
     9961 cagatcggct gttttcaaac tggcaaatca tttcgcctac gcgtttggta gctcccatga
    10021 ccgacgaggg gtttacggtt ttatcggtcg acacgaacac aaacttttct gcgccgtatt
    10081 tgtctgcggc ttttgccacc acaagagtcc caaaaatatt gtttttcact gcctctgaaa
    10141 tattttcttc cattaaagga acgtgcttgt acgctgccgc gtggaacacc agctgcggcc
    10201 ggaagttctc aaatacgtat tccaccctgc tcttatcctg gatatttcct acaaccgggc
    10261 gcacctggag attgggaaag ctgcggcgca actcgtgaag catttggaat attcctgttt
    10321 catcctggtc taaaagcaca agagcttcgg ggtgaaaacg cgccacctgt ctgcataatt
    10381 cagacccaat ggaccccgca gcccccgtaa tgagtacagt ctttcctgca ataaaccgct
    10441 caatgagttc ggcgtgcaaa tgcacaggct ctctccccag caaatcctcg acctgcactt
    10501 cgcgcagagc gtctaccggc acgccttttc gcacaatgtc ctggagcgtg ggcaccacgc
    10561 gcatgttgcg caggccggct tctctcccct tttctaccgc ccgtttgatc gcctgggctc
    10621 cggccgaagg caaggcgata atcatttctt ctatctgata ctgtgcaacc acgcgcggga
    10681 tatcgttgct tgtaccaact actttaatgc cgtggataac cactcccttt ttaatagggt
    10741 tgtcgtccac gaacgcaaca ggccggacat tctgcctccc tgccgccaag cttctcacta
    10801 actgctcccc cgcgtctccg gctcccacaa tgagagttct ttttccgttc tctgtttcct
    10861 gattctcaac tacctgcata tatattcgtt ttgccatacg caaagcgcct atcgaaaaaa
    10921 atgcaaacac atagctcatc aaaagcgtcg agcgcggaaa ttcctggaaa agttcggtgt
    10981 cccggaggaa taatacgatt cctgccagca gcgcaaaccc aagggttacc gctttcccca
    11041 acgaaatgag ttcttcggtg cttacatacg cccacgacaa ccagtacaaa tggaaaaagt
    11101 acagcagagg cagaagaaaa acaaccgcaa gcaaaatcgt agtctgaatt gctcccgacc
    11161 aataggaagc aggtatggaa ccctcaaagc gcagaaaaaa agcggcaacg accgaaaaag
    11221 caatcagcag cgtatctgca gcaacaaaaa aggcaaagcg tgttcgtgtg gtgcgttgaa
    11281 acttaggaaa cgtcatggag tgcgtgctgc aacgtcgtaa ctacggtatc gatatcttgc
    11341 tctgaaagat tattaaaaaa cggcaaagca atagtacgat tccctacgct ctctgtaaca
    11401 gggaaatccc cttctgtata tcccttctgc cgataaaacg gttggagatg aataggcaca
    11461 aagtatggac ggcattgcac gcctttgttt tcaagatagg ccaaaaccgc atcacgctgc
    11521 tctttcgtat acgacttttc caaacgaatt acatacacaa accagctcat cataacgtct
    11581 gggctcaccc aaggcacttg aactcccgac agctcttgta acttttctgt gtactgattt
    11641 gctacccgat ctctcttctg caagatttcc tgaagccttc ccagctgcgc aacgccaagt
    11701 gcgcatggta tttctccgag gcgatagttg tatcccagcc gtacgtgttc taaccactct
    11761 cctcctttca ctttgcgacc ctggtttgcc atgctttggc ataattcagc tgtgatattg
    11821 tcgtctacaa gaatcatgcc cccttctcct gtggtaatct gcttgttggg ataaaaagaa
    11881 aatatggcgg cgttaccgaa acttccgcac tttctgccct tgtacaatcc tccgattgcc
    11941 tctgcggaat cttcgataac aaacaacttg tgtttttctg ctatttgtaa aatcgcatcc
    12001 cagtctgccg agtgaccaaa cacatcgact gccaaaatgg ccttggtctt ttgggttatg
    12061 gcagcttcta tctttgcagg atcgatttgt agcgtttgct cgtcgatatc gcagaacaca
    12121 ggcgttgcgt tctcaaataa aatgcagttt gaagaactga taaaagaaaa aggcgtagtg
    12181 atcacttcgt cgccctcacc aatgcccaaa gcccgaacaa taagatgcaa cgctgcggtg
    12241 ccggagttta ccgctatggc atatttcctg ccgacatact gcgccacttt ctcttcaaac
    12301 tctttttgct tgggccccaa acttaatgtt gcagtctcca acacttcttg cactgctttt
    12361 cgttccgctt ctgtaatatc gggcgccgat aaaggaatca tagttttttc acgactttgg
    12421 cgggaactcc gaaagctacc acgtttgccg ggatgtcttt ggtaacaaaa ctcaaagcgc
    12481 ccaccacgga attttctccc acggttactc ctggcattac cacactatga gcaccaattt
    12541 tgcagttttt tttgagtacc actttcccct ctttttcgtc tatggtggaa acagaataaa
    12601 tcgaacagtg ggaaccaatc tgcacgttat cttcaattac tatgccgttt tttgcattta
    12661 tgtaacaaaa agctcctatg tcggtatact tgccgagccg gaggttcttt gcatgctgca
    12721 ccatccagtt atacttcgta agctttcctt cttcgatttt tggcggcttc cagccggaaa
    12781 agcgcttcat attatttata gtcttccact accccgtttg ctccatactg cccttgcccc
    12841 gagaacacgg ctttggccgt taagagcaca atcttgatat ccagccacag agaccagtgt
    12901 tcaatgtacc atatatccca ctcgattcgc tccttccacg acaacctgtt ccaccctttg
    12961 agcatggcca tgtttgccat gccaggtttt acctcaagcc ttcgccgttc tcttgttgaa
    13021 tactgtgcca cttggtgcaa aagcgcgggg cgggggccca caaaagacat tgtgccaaag
    13081 agaatatgaa caatctgcgg ggactcgtct aacgtaaaac gccgaagaaa ttttcccacc
    13141 ctggtaatgc gcgtatcgtt ttgctcaacc tcgataccct ttccaagttg tattgcgttg
    13201 tctaccatgc tcctgaactt catcatacga aagggcttgc cgtcttttcc gattctctcc
    13261 tgaataaaaa ataccggtcc tttggaatcc agctttatgg caccggcgat taacacaaaa
    13321 gggatt
//
LOCUS       DASRUP010000003         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1591372, whole genome shotgun sequence.
ACCESSION   DASRUP010000003 DASRUP010000000
VERSION     DASRUP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591372"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFE94_00140"
     CDS             complement(<1..357)
                     /locus_tag="VFE94_00140"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HZX49548.1"
                     /translation="MTLSTPIGQVPRIGTAYEAKLKKLGITHVRDLLFHFPRTYEDFS
                     KITPIQDVQEGQVYCVAGSLLEINEVRTYRKRVSLITGMVEDDSGAIKILWFNQPYLA
                     DSLKKGDNVYLAGKVMR"
     gene            complement(385..456)
                     /locus_tag="VFE94_00145"
     tRNA            complement(385..456)
                     /locus_tag="VFE94_00145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:gcg)
     gene            513..599
                     /locus_tag="VFE94_00150"
     tRNA            513..599
                     /locus_tag="VFE94_00150"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:547..549,aa:Ser,seq:gct)
     gene            610..1461
                     /locus_tag="VFE94_00155"
     CDS             610..1461
                     /locus_tag="VFE94_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZX49549.1"
                     /translation="MPEILQNIVTAATPWLLGHGITIAAILIVAYAIRRFARVFIEKL
                     VRKLIVSDRFLSKQAEQKREDTLIQIGTTSLSILVWLLAIMMVLQETGVAITPLLAAA
                     GVAGLAFGFGGQYLIRDLISGLFIIMENQYRIGDVVCFDKTCGLVEDITMRMTTLRDL
                     DGVVHHVPHGEIKTVSNLSKYYARVNLNIGISYHTNLEHAISVVNKVGQELAEDPEWK
                     EHILKAPQFLRVDDFADSAVIIKILGDIKPLMQWDVAGELRKRLKIAFDKEGIEIPFP
                     QRVIHQI"
     gene            1469..>2678
                     /locus_tag="VFE94_00160"
     CDS             1469..>2678
                     /locus_tag="VFE94_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HZX49550.1"
                     /translation="MNKIFFILAGVLVLLIGGFFLLNNYIYNEKQPDPETGTVPAEIV
                     EAFQNKLVARGVERQGQPIEGFDAFLLLQAFPKLESADFDGVLTFEGVYTYENGELVY
                     QRKAGMPVTSAEQTVSIEGYEILLQNLSQRFGVNVQDKQAVLDLVDTLEGTPSTPPPA
                     DQPITIKGEITCLPKKGSGPQTLECAIGLRGEDGNHYALKNLFDYDPNYTFSTTGMQV
                     EVVGIFTPEQVSGPGNSTYDVVGSIAITSIKEDSQGQEISLREGQRESSFLLQNIYPD
                     RVEGLNYWEYPVATDQGHPITLRPGEVVSNGCTVQLTLLRIEGNTAVFEKKVDFNRPC
                     PICLAKDTLIDTPAGKVAVQNLQKGDAVWTVDKNGQRVAGVILEAAKTPVPATHQMVH
                     VVLQDGRELFV"
BASE COUNT          727 a          615 c          653 g          683 t
ORIGIN      
        1 gcgcattact tttcccgcca aatacacgtt gtcgcctttc tttaaagaat ctgcaagata
       61 gggttggtta aaccacaaaa tcttaatcgc cccagaatcg tcttctacca ttccggtaat
      121 taaggacacc cgttttcggt acgtgcgcac ttcgtttatt tccaaaaggc ttccagccac
      181 gcagtacacc tgcccctctt gtacgtcttg tatgggggta attttagaaa aatcctcgta
      241 ggtgcgcgga aaatgaaaaa gaagatcacg aacgtgtgtg atccccaatt tctttagttt
      301 tgcttcatac gcagtgccga ttctcggcac ctgcccaatg ggagtagata gcgtcattgt
      361 gtttacactg agcttgtcga agtgtgcgcc cgacaggaat cgaacctgta tcgagggatc
      421 cgcaatccct tattctatcc gttaaactac gggcgcgata tttcatatca taccttgatt
      481 tttaacttat ttcaagtaca atagagcctg caggagaggt ggctgagtgg tcgaaagcgg
      541 cacactgcta acgtgttatc ccttaaccgg gatcgagggt tcgaatccct ccctctccgc
      601 tacatgccta tgccagaaat attgcaaaac attgtaaccg ctgcaacacc ctggctttta
      661 ggccacggca ttacaatcgc ggcaatcctt attgtggcct atgccattag gcgatttgcc
      721 cgagttttta tcgaaaaact tgtacgcaag cttatcgtct ctgatcgctt cttaagcaag
      781 caggccgaac aaaagcgcga agacacgctt attcagattg gaactacgtc gctaagcatt
      841 ttggtgtggc ttttggcgat tatgatggtg ctgcaggaaa ccggagttgc cataacgccg
      901 cttctggcgg cagccggcgt ggccgggctc gcgtttggtt ttggcgggca gtacttaatt
      961 cgcgacctca tttcgggctt gttcattatt atggaaaacc agtaccgcat tggagacgta
     1021 gtttgcttcg acaagacatg cggcttagta gaagacatta ctatgcgcat gaccacgctt
     1081 cgcgatctcg acggcgtagt acaccatgtg ccccacgggg aaattaagac cgtatcaaat
     1141 ctttccaagt attatgccag ggtaaacttg aacatcggca tttcttacca caccaacttg
     1201 gaacacgcca tttcggtggt aaacaaagtg ggccaggaat tggcagaaga ccccgaatgg
     1261 aaagagcata tcttaaaggc cccgcagttt ctgcgcgttg atgattttgc agactctgct
     1321 gttataataa aaatcttggg agacatcaaa cccttaatgc aatgggatgt tgcgggcgaa
     1381 ttgcgcaagc gcctaaaaat tgcgttcgac aaagaaggca tagaaattcc gtttccccaa
     1441 cgcgttattc atcaaatata attattgtat gaacaaaata ttttttattc ttgctggagt
     1501 actcgtgctg cttataggcg ggttcttttt gcttaataac tatatctaca acgaaaagca
     1561 gccagatccc gagacaggca ctgttccagc agaaatagta gaggcgttcc aaaacaaact
     1621 cgtagctcgt ggagtagagc ggcaggggca gcccatagaa gggttcgacg catttttgct
     1681 tttgcaggcg tttcctaagc ttgaaagtgc agatttcgac ggcgtgttaa catttgaagg
     1741 ggtgtacacg tatgaaaatg gagaacttgt gtaccagcga aaggcgggca tgccggttac
     1801 atcggccgaa caaacggtat caatagaggg ctacgaaatc cttttgcaaa atctttccca
     1861 aaggtttggc gtaaacgtac aagataaaca agctgttttg gatcttgttg atacgttgga
     1921 gggaacaccg agtacgcctc cgccagctga ccaacccata accataaagg gcgaaattac
     1981 gtgtttgccc aaaaagggtt ctgggccgca aaccttagaa tgcgccattg ggctaagggg
     2041 tgaagacggc aaccactatg cgttaaaaaa tcttttcgat tacgacccaa actacacgtt
     2101 ctctaccaca ggaatgcagg ttgaagttgt aggcattttt accccagagc aggtatcggg
     2161 cccgggaaat tccacctatg acgtggtagg aagcattgct ataacttcta taaaagaaga
     2221 ttcccaaggc caggaaatat cgttgcgcga aggccagcgc gagagttcgt ttttgctgca
     2281 aaacatatat ccagaccgcg tggaaggatt aaactattgg gaatacccag tggcaacaga
     2341 ccaggggcat cctataacct tgcggccggg agaagtggta agcaacggct gcaccgtgca
     2401 acttacgctt ttgcgcattg aaggaaatac tgcggtattt gaaaagaaag tggattttaa
     2461 ccgcccatgc cccatatgtt tggcaaagga taccttaatc gacacgcctg ctggcaaagt
     2521 tgcggtacaa aatttgcaaa agggcgatgc ggtttggaca gtagacaaaa atggccagcg
     2581 cgttgcaggt gttattcttg aagcagcaaa aactccggtg cctgccacgc atcagatggt
     2641 gcatgtggta ttgcaagacg gcagagaact ctttgttt
//
LOCUS       DASRUP010000004         5161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_954914, whole genome shotgun sequence.
ACCESSION   DASRUP010000004 DASRUP010000000
VERSION     DASRUP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954914"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..969
                     /locus_tag="VFE94_00165"
     CDS             52..969
                     /locus_tag="VFE94_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49551.1"
                     /translation="MDTRDLEELLEKARLCVEKNKGIVLPEHTPYLGMGASYYAALTL
                     WFLGKDILPYEAFEYYAYQGKPKEKLGVLISQSGESSELLWNLARFKDVVAITNDAES
                     SLAKAVNAKQVVELYAGKEEFVSIKTYFNTLLVLYLGLGFDPRKVLDAIEKQFSAYRG
                     QAQERAQEIAEYMRGRKLKGLFVLGSGPNAPTAMEGALTLSEATKFAWQGLSPAQYDH
                     GPKETMQDSVVLFLQSNGKDKERIAHLKKLLSAKSNALVREIVVSGVPEELSPFCHIV
                     QLNFLMVFLTKALGVKAEFMGEKITRTPL"
     gene            966..1430
                     /locus_tag="VFE94_00170"
     CDS             966..1430
                     /locus_tag="VFE94_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZX49552.1"
                     /translation="MSNDKEKHTIIATSGGFDPLHIGHVRLFREARALGDELVVILNN
                     DNWLLKKKGYVFMPQEERKEILEALESVDRVIITSHTPDPADMSVCQELRQLGPHIFA
                     NGGDRKEDNIPETQVCNELGCKMVFNVGEGGKVQSSSWLVEKYRKQHGTDDT"
     gene            1411..2013
                     /locus_tag="VFE94_00175"
     CDS             1411..2013
                     /locus_tag="VFE94_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZX49553.1"
                     /translation="MEPTIHKEAKALVVVAHPDDETIWMGGTLARHSTVDWTIFVLCR
                     KSDPDRMPKFMRVAKYYGARGIICDLEDEGIMSIKESILEIQKIVRSRLAQKKFDYIF
                     THGAKGDYGHPRHIGAHRAVKKMIAEKELEAREIFYFAYKLDEKKKIAVPLENARYAV
                     ELTNKEWQAKRNVIKKLYGFRPHIFENRSCSKIETFTSKA"
     gene            2032..3177
                     /locus_tag="VFE94_00180"
     CDS             2032..3177
                     /locus_tag="VFE94_00180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZX49554.1"
                     /translation="MKSLVLYAYPPETDGLSLQGDMLYRGMKANGEEVMPCHWLASFQ
                     KEWLYETFKPQFALGIGFWGYTPDLILHPQKFGVTPVPWFVADGWVANYQELLGSLPL
                     VFVTSDWVRRTYQRDGVDTKNFVTAHIGVEPELFRPIAKTDPAIQKLRSMWGVKPNEK
                     VILTMGGDVTSKGAQEVFRALAKIGTRFENWKYICKAWDEEDERDHAEEELRLIEELG
                     LPKEKILFLSGSWSREFIPYALSATDVYAAPSRLDGYGMIQVEAQACGTPVVSINEMG
                     PKETVLHGKTGFLANVAETVDLESEIIYPEMGFKKQGRIVFDEPKTFAYRANTDELAE
                     YLYTLLADDEKRLQMGQAARVHAVANFDYRNLARQMTNTIKERLHLL"
     gene            3174..4064
                     /locus_tag="VFE94_00185"
     CDS             3174..4064
                     /locus_tag="VFE94_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HZX49555.1"
                     /translation="MKIQAEKYPGNPILTPRVEDGSFEKACAYNPAAVVKGDKVYLLY
                     RAEDGYYDRYISRIGIALSEDGFHFERYGENPILEGEGKEESRGIEDVRVTQTEQGYF
                     ATYTGYSGRDEQGQRIVLCGAFSKDLIHWEKIGRLVPGREKAGAIIQDYTHKGKYVMY
                     FGEGMVKIAFSEDLRNWEVQEETVLEPREGFFDSHLVEGGPPPLVTDEGILLIYNSSN
                     GVSYQPGFALFDKEDPAKLLYRSDKPILEPTEYWEMYGKVNAVVFTNALVNFKGKWLL
                     YYGGADKSIGVAEVTFARED"
     gene            complement(4042..>5161)
                     /locus_tag="VFE94_00190"
     CDS             complement(4042..>5161)
                     /locus_tag="VFE94_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49556.1"
                     /translation="QKAQMKNILRMHAVSSGFAGALIGIFIPIYLLGLGKPLAEVMLF
                     LIVHNIVLFGAALCAVFVSNKVGLVRTLWIRFGFLFLYLFLLYLLQTETGFPIYLVAV
                     VGGIEAAFYWIPLNILFTRYAETKAMAKQMSKFFVLPEAAGLFAPLIGGFIAVSLGFP
                     ALFVFAFIVNIAAVGFLQNLHKEKTNFEFSLSRFKRIWQRNKTYFVSEFFDNIPEEVV
                     GIIFPIVAYLSLLSVTDIGVIGTLISLGMMIFTLLIGNFADRFDKIKMLRLGAALFIA
                     TWLVAFFMESVLGFYAVSILLGFFLRLFLVPYNALLYANAKTDDAQFLVLREIPVISA
                     RVFVFLLAILLASRLEFVFLAAALSLLYFFFFNPRGQK"
BASE COUNT         1499 a         1128 c         1367 g         1167 t
ORIGIN      
        1 cgccccgcca gtctatagac tggcggggtt tgtttttgct agaatcaagg catggatact
       61 cgggacttag aagaactttt agagaaagcg aggctttgcg tagagaagaa caaaggtatt
      121 gtattgcccg aacatacccc gtacctcggc atgggggctt cctattacgc ggcgcttacc
      181 ctgtggtttt tgggcaaaga cattctgcca tatgaggctt ttgaatatta tgcctatcag
      241 ggcaaaccaa aagagaaact gggcgtgctt atttcccagt ctggagaaag ttcagagttg
      301 ttgtggaatt tggcgcgatt taaagatgtt gttgccatta ccaacgacgc cgagtcttca
      361 cttgcaaaag ctgtcaacgc caaacaggta gtggaactgt atgccggcaa agaagagttt
      421 gtttctatca aaacctattt caatacgctc ttagtgctgt accttggttt ggggtttgac
      481 cccagaaagg ttctcgatgc catagaaaag cagttttccg cgtatcgcgg ccaagcccag
      541 gaaagggcgc aagaaattgc agagtatatg cgggggcgca aactcaaagg gctttttgtg
      601 ctgggttcgg ggccaaacgc gccaaccgcc atggaagggg cgcttacctt aagcgaagct
      661 accaagtttg cctggcaggg actgtctccc gcccagtacg atcacggccc caaagaaacc
      721 atgcaagact cggttgtgct gtttttgcag agcaacggca aagataaaga acgcattgcg
      781 cacttgaaaa agttactctc tgcaaaatca aatgcgcttg tgcgagaaat tgtggtcagc
      841 ggagtgccag aggagctttc gccgttttgc cacattgtac agcttaactt tcttatggta
      901 tttcttacga aagcgcttgg cgtgaaggcg gagtttatgg gagagaaaat tacgcgcacc
      961 cctttatgag caatgataag gagaagcata ctataatcgc aacaagcggg ggcttcgacc
     1021 ctttgcatat tggccatgta cggctttttc gcgaggcgcg ggcgttgggc gacgaactgg
     1081 tggttattct taacaacgac aactggctgc tcaaaaagaa aggatatgta tttatgcctc
     1141 aagaagagcg caaagaaata ctcgaggctt tagagtcagt tgaccgggta attattacca
     1201 gccacacgcc agaccctgcc gatatgagcg tttgccagga actgcgccag ctcggacccc
     1261 atatctttgc caacggaggc gaccgcaaag aagacaatat tccggaaacc caggtatgca
     1321 acgaactggg atgcaaaatg gtgtttaacg ttggcgaggg cgggaaagtg cagtcttcct
     1381 cgtggcttgt agaaaagtat agaaagcagc atggaaccga cgatacataa agaggcaaag
     1441 gcgttagtag ttgttgccca tccagacgac gaaaccatat ggatgggcgg tacgctcgcc
     1501 cggcattcga ctgtagactg gaccattttc gtgctttgca gaaaatcaga ccccgatcgc
     1561 atgcccaaat ttatgcgcgt ggcaaagtac tacggcgccc gcggcattat ttgcgacctg
     1621 gaagacgagg gaataatgag cataaaagaa agtatcttag agatacaaaa gatagtgcgc
     1681 tcgcgccttg cgcagaaaaa gtttgactat atctttactc atggcgccaa aggcgactat
     1741 gggcatccaa ggcatattgg cgcgcatcgc gcggtaaaaa aaatgattgc agagaaggaa
     1801 ctggaagcga gagagatttt ttactttgcc tataagctcg acgaaaagaa gaagatagcg
     1861 gtgcctcttg aaaacgcaag atacgccgtg gagctgacga acaaggaatg gcaagccaaa
     1921 agaaacgtga taaaaaagtt atatgggttc aggccccata tttttgaaaa ccgcagctgc
     1981 agcaaaatcg aaacctttac gagtaaggct tagccttact caactttata catgaaatct
     2041 ctagtgttat acgcgtaccc tcccgaaacc gacggtttga gcctgcaagg cgacatgctg
     2101 tatcggggca tgaaagcaaa tggggaggaa gtaatgccct gccattggtt agcttccttc
     2161 caaaaagaat ggctgtacga aacgtttaag ccgcagtttg ccttgggcat tggtttctgg
     2221 gggtacacgc cagaccttat tctgcacccc caaaaattcg gggtaactcc agttccctgg
     2281 tttgtggcag acggctgggt ggcaaattat caggaacttt tgggcagttt gcctttggta
     2341 tttgttacgt cggactgggt gcgtagaact taccaaagag acggcgtgga caccaaaaac
     2401 tttgttactg ctcacattgg cgtggaacct gaacttttcc ggcctattgc taaaacagat
     2461 cccgcgatcc aaaaactgcg ctccatgtgg ggcgtaaagc caaatgaaaa agttattctt
     2521 accatggggg gagacgttac ctcgaaaggc gcgcaggaag tgtttcgcgc gttggcaaaa
     2581 ataggcacac gttttgaaaa ctggaaatat atttgcaagg cctgggacga agaagacgag
     2641 cgcgaccacg cagaagagga gttgcggcta attgaagaac ttggattgcc caaagagaag
     2701 attctgtttt tgtctggttc atggtcgaga gagtttatac cctatgcgtt aagcgccaca
     2761 gacgtgtatg cggctcccag ccgcctagac gggtacggca tgattcaggt ggaagcccag
     2821 gcctgtggca ctccggtggt gtctattaac gaaatggggc ctaaagaaac ggttttgcac
     2881 ggcaaaaccg gctttcttgc aaacgtggca gaaaccgtag atttggaaag cgagattatt
     2941 tatccggaaa tgggttttaa aaagcagggc cgcattgtgt tcgacgagcc caagaccttt
     3001 gcctatcgcg ccaataccga cgagttggct gaatatttgt atactctgct cgcagacgac
     3061 gaaaagcgtt tgcaaatggg ccaggccgca agagttcacg ccgtggcgaa ctttgactat
     3121 cgcaaccttg ctcgccaaat gactaacacg attaaagaac gtctccacct tttatgaaaa
     3181 ttcaagcaga aaaatatcca ggcaatccca tactcacccc gcgcgtagag gatgggagtt
     3241 ttgagaaggc gtgcgcgtat aacccagctg cggtggtaaa aggcgacaag gtatatcttc
     3301 tctatcgggc agaagacggg tattatgatc gttatatttc ccgcataggg attgctttga
     3361 gtgaagatgg gttccacttt gagcgttatg gagaaaatcc gattctcgaa ggtgagggaa
     3421 aagaggaaag cagagggatc gaggatgtac gcgttaccca aacggaacaa ggttattttg
     3481 ccacctatac gggttactcg gggcgggatg aacaaggaca gcgcattgtg ctttgcgggg
     3541 ctttctcaaa agatttaatt cattgggaga aaatcggccg gctggtgcct gggcgcgaaa
     3601 aagcaggcgc gattattcaa gactatacac ataaagggaa atatgtgatg tactttggcg
     3661 aaggcatggt gaagatcgct ttttctgaag atttgcgaaa ttgggaagtg caggaggaaa
     3721 cggtgttaga gccgcgcgaa ggctttttcg acagccattt ggtggagggc ggcccgcctc
     3781 cacttgtgac cgacgagggc attttgctga tttacaacag ttcaaacggc gtatcctacc
     3841 agccgggctt tgcccttttc gacaaagaag acccggcaaa acttttgtat cgttccgaca
     3901 aaccgatatt ggaacccacc gagtactggg aaatgtatgg gaaagtaaac gctgtggtat
     3961 ttacgaatgc cttggtgaac ttcaaaggca aatggctttt gtattacggg ggagccgaca
     4021 aaagcatcgg cgtcgccgag gttacttttg cccgcgagga ttaaagaaga agaaatagag
     4081 aagcgaaagc gcggctgcga gaaacacaaa ctcgagccgc gaggctaaaa gaattgcgag
     4141 aagaaatacg aaaacgcgag ctgaaattac cggaatttcg cgcagtacga gaaattgggc
     4201 gtcgtctgtc ttggcgtttg catacaggag ggcattatag ggcaccaaga acaggcgtag
     4261 gaaaaaccca agcaatatgc tcacggcgta aaatccaaga acgctttcca tgaaaaaggc
     4321 aaccagccac gttgcaatga agagtgccgc tcccaaacgg agcattttta ttttgtcgaa
     4381 gcggtcggca aagtttccta ttaaaagagt gaatatcatc atgcccagcg atatgagggt
     4441 gccgataaca ccaatgtcgg ttacactcaa taagctcaag tacgccacaa tgggaaagat
     4501 gattccaacg acctcctctg gaatattgtc gaagaattcc gaaacaaagt aggttttatt
     4561 gcgctgccat atccttttga atcgagataa cgaaaactcg aagtttgttt tttctttatg
     4621 cagattttgt agaaagccaa ccgcagcaat attcacgata aaagcaaaaa caaagagcgc
     4681 gggaaagccg agagaaaccg caataaatcc accaatgagg ggggcaaaga ggccggcggc
     4741 ttcgggcaga acaaaaaact tgctcatctg ttttgccatg gctttcgttt cagcatacct
     4801 cgtaaaaagg atattgaggg ggatccagta aaacgcagcc tcaatgccgc caacgaccgc
     4861 aaccaggtag atagggaagc cggtttctgt ttgcaaaaga tataaaagaa aaagataaag
     4921 aaagagaaag ccaaagcgaa tccataaggt gcgcaccagc cctactttat tggaaacaaa
     4981 aacagcgcat aacgccgcgc caaacagcac gatattgtgc acgataagaa agagcataac
     5041 ttcagcaagc ggcttgccga ggccaagcaa ataaatcggg ataaaaatgc ctatcaaggc
     5101 tccggcaaac cccgagctta cagcatgcat gcggagaata tttttcatct gcgccttctg
     5161 t
//
LOCUS       DASRUP010000005        14670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1104872, whole genome shotgun sequence.
ACCESSION   DASRUP010000005 DASRUP010000000
VERSION     DASRUP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14670
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104872"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..961)
                     /locus_tag="VFE94_00195"
     CDS             complement(92..961)
                     /locus_tag="VFE94_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HZX49557.1"
                     /translation="MQSFLEFESIKKFIDTAGKDIEKYFGKDKGALIYLKPNGVWYAQ
                     GLYEWLTKEKKKDIIISTMEDDGTGLEAAKLRGRKVLIVDADVVTGKAYKRSMEALRL
                     CKKELELKEIRFATFIDRIGLADFSVWKYSPESIWHFDEMNVLDLKIIAHLAGNGRET
                     FADIGKAINLSAVTVKNRVDKLLRERTIAIQAQLQADQFYTMSVQIFLEADEQTVKKL
                     IEKCEPKQEVYHMVRITGIYNLGIGLLAYNWHAIEEFIEREVRAEKGVKKIFIITGQL
                     PIFPKTIPPKILQ"
     gene            complement(972..2219)
                     /locus_tag="VFE94_00200"
     CDS             complement(972..2219)
                     /locus_tag="VFE94_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HZX49558.1"
                     /translation="MKFVGDFHIHSKYARATSQDMDLEHLDTWAKIKGIKVISTGDFT
                     HPVWFKELQEKLEPVEQGLYQLKQSKLGTRFILTTELSCIYSKGGQVRKVHLLIFAPS
                     LEAVAKINAHLGVRGNLKADGRPIIGIDAKEVVKIALDASPDCMVIPAHAWTPWFAVF
                     GSKSGFNSLQECFDEYTKYIYAIETGLSSDPAMNWRLSQLDSIAFISNSDSHSPPKIG
                     REANVFDTELSYQGIADALKKKDPSKFLFTVEFFPEEGKYHYDGHRLCGVSFKPEESK
                     QHNNICPKCGRPLTIGVLNRVAELADRPEGFRPEGAIPYKSLIPLEEIIADALGVGTV
                     SKRVREEYEKLIHTVGDEFSILLDADIAGLQSATLPEIAEGIKRVREGNVHIEPGFDG
                     EYGKIEIFKEGEPKTFTTQKSLF"
     gene            complement(2620..3486)
                     /locus_tag="VFE94_00205"
     CDS             complement(2620..3486)
                     /locus_tag="VFE94_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZX49559.1"
                     /translation="MSTVLFAKTALAIYGLGAVFVLALLLEVWNTDKSLLLASLSQHL
                     VVEEINAERRNIGLSPLRVSAALQQASQLKAEDMVAQGYFSHQGPNGEEAWVWLDRVG
                     YSFAGAAENLAIDVADSKVLVQAWLHSPNHAKQLQNPDFREVGIGFAEGIIDGRETRV
                     AVAFLGWPLPSNQILSSPTVSNIVVQEPEISIEPIPPREPLAMVRVEVKDTELNEPLR
                     MQVAGSQLSASRDWGGIARLLLSVLYVGLLAWLLVLLWQQKKAFALPRIAYLSLLLLM
                     LWSLELGRLWYF"
     gene            complement(3501..6485)
                     /locus_tag="VFE94_00210"
     CDS             complement(3501..6485)
                     /locus_tag="VFE94_00210"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HZX49560.1"
                     /translation="MEYISFLTNKKSVVHAFLLLFAFSGMVFSASFIPAGNGFVSYQT
                     VLAEEKGYEDAHETENEEEEPVETGGGDACVPNPEADLFGSIQTGTGNASIGNRSEKC
                     AYEVGLASYKMFDQNIDNQELFGSDTAIVAAGSEVDLVVDLPECAYQIDLFYGDLIES
                     FAGGVRYGDRLLDGDQGKESDFCEKESDTFSISGMKFEDKDKDATKEAGEGGLQGWEI
                     KIEGPNAFSDSTTTNADGNYEFSDLEAGVYEVCETQQTDWDQTFPTDNNGCHEVTLAD
                     KDVEDIDFGNAKEPEQLIKKLTIVASKIVCEDPSDFPDWSDAGVDIAKDTAQDFVASN
                     PGCSVVKDWEFQWGYANVNENKQSGDHVGEASATGTADNEWKTFGPTNANGVATVEIT
                     DTKGTEKIWVREVLQAEYIAFSDWPNVTGTDQSDPSARMWCHTDVFKHDNHDFISSPE
                     LGRTYYCLAFNALKPVVQPATFSISGMKFNDENEDGVKDGTEVGLQGWDITITDGAAF
                     NDSTTTDANGEYEFTDLEPATYTLCETQKAGWNQTFPTANNGCHEVIITNADISDIDF
                     GNAKEEELGAEPEPEPEPTPTPTPTPTPTPTPSPTPPSGGGGGGGSVRLPALRIEKSI
                     AESFTNPGGTVNYTIEVSNIGPVTAINTVLTDTLPAGFLHKETSQQNRTWQLGGMVPN
                     AQQTVTFEVLVGQEVQVGSYTNTAEVKADNHQPLQAKATIEVRVPGTQGEEFKPALVI
                     EKTIDKPVVQRGEQVIFTITLRNTGKATALNVLVIDTLPPGFTSPLAERLRVWTIPQL
                     APGQVWQETLVATAKQDAVLGVHMNTVVARAENHPQVEDSVGVIVQAPTLPHTAMGVV
                     QEESAETRSENFLTIPSIGVHIPIVEGNDDSALESGAWRIPGTSTPDKGSNTVVGAHR
                     FKYLPPYSETFYLLDKLQAGDRFEVVWEGKTYTYEVSDSRVVEATALEVLQPTEEAVF
                     TMITCISLAPDRGRIVVQGVLL"
     gene            complement(6826..8400)
                     /locus_tag="VFE94_00215"
     CDS             complement(6826..8400)
                     /locus_tag="VFE94_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZX49561.1"
                     /translation="MNVRISFIAMNMAFALVLVVGALWAANAIYFEQSYFIMMFKYPW
                     YQLTLSGDRGGSFLASVFPISQSKELESEGGGKALSIPVLVYHGIVGKAEGDNVTTRD
                     FKEQMMALKDAGWETVSIEEFQAFMKGELQLSEKSFLLTFDDGRKDSYYPVDPILRAL
                     DYEAVMFVITNQSIGEKGKATHFYLSEKELKHMQSTGRWDIQSHGKFAHELYATGPNG
                     ETGHFYSNLLWVPEEERPETPEEFQERIYEDLRISKEEIEESLGISVATIAFPFGDFG
                     HNLFADSNFELAKGILLISAKEHYSHGFYQWWEGEGFTFNYPREDVLTVKRLEVLPNW
                     SGEALLQILEQGMSKSLPYQESFLQNNAWLSNWGSFDMQPGKGLVFLAEGGQAGASAV
                     LDGTGGWEDYEVKARVNLPSQTGVFIWVRFQDANNNAACNFGLDFVHAEQVFKGEKRV
                     IKGVRGVTPLKEGDFTVSARVQGRTIQCLVEGEIVVESTFLEPDLNRGGVGFKMWDEQ
                     IGKSQLLVRSLEVQPL"
     gene            complement(8411..9772)
                     /locus_tag="VFE94_00220"
     CDS             complement(8411..9772)
                     /locus_tag="VFE94_00220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX49562.1"
                     /translation="MPSPVIIPKKQMPTPSRRIFSGEAKKVILRELFILLFLYAVGLG
                     VLFLIVLLKRNSAFNFFDNPFLYGYTFFVTIFRISRIWAALFYEKADKKLLEPYAQMH
                     YEPAISFVIPCKNEEAAIEKTVTKCFEARYPPEKVEVIVVNDGSTDSTGAILGKLQFR
                     FPNLTVVSWEQNRGKRHGMAEGFRRARGEVVVQLDSDSYIEPGTIQKLVDYFKNPQVG
                     AVCAHTYVENADQNILTRMQTAHYFVAFRVLKAAESAFSLIFCCSGCSSAYRKSVVLP
                     VIDEWLNESFLGVPANWGDDRALTNRVIRQGYKSVYTDKAWSFTIVPDTWKKFVKQQI
                     RWKKGWFVNSLFAGKFIMKRDSFVAVTYFFPLFLTHFLSPFIAVKVFMVDALIFGVSP
                     LYYIIGASSVIIFFIIFYKILRPEDKYWPYLIPWALLNALFLSYVLFWALATIRNRGW
                     GTR"
     gene            complement(9777..11087)
                     /locus_tag="VFE94_00225"
     CDS             complement(9777..11087)
                     /locus_tag="VFE94_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZX49563.1"
                     /translation="MNASKKRKILPPSGGTVAVVGLGYVGLPLALLAERKGYRVIGID
                     IAKERIRLLSARIMPFQEDGLVPFLKKSKMEATDNFAKIREADIIIICVPTPVHEDHT
                     PDFTPVKEATKRVAEFLRKEQVVILESTVSPGSSENIVIPLLEKISGLRAGQDFSFAH
                     CPERINPGDPQWNIEYIPRVVGGVDERSTEQAVGFYRSILQGPVTQMGSVKEAEAVKI
                     VENSFRDINIAFVNELAMSFATMGIDVTNVLQGASTKPFAFMAHDPGCGVGGHCIPVD
                     PYYLIDTAKQNGFLHRFLSLARSINAGMPKYAADLLTEGLRERNIPVKGTKTALLGLA
                     YKPDIDDDRESPSYEIEKCLKQQGLRVATFDPFVLQRSSAASLEKALKGARAVVVATA
                     HTAFCNLEPEFLHSRGVQVVVDGRNCLSKEAFLQSEVLYKGIGR"
     gene            complement(11382..11774)
                     /locus_tag="VFE94_00230"
     CDS             complement(11382..11774)
                     /locus_tag="VFE94_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX49564.1"
                     /translation="MNQPYQRKKVLVIEDEDLLRGILVEEFTEQEVDVVAARDGLEGL
                     ELALAEQPDLIILDLVMPRLDGMSMLKKLREDEWGKQARVLILTNAEGDFEKTMEALR
                     HDVYEYMVKTRWGLKDVVGRAKEKLGIV"
     gene            complement(11771..13900)
                     /locus_tag="VFE94_00235"
     CDS             complement(11771..13900)
                     /locus_tag="VFE94_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX49565.1"
                     /translation="MIFKKITQHPLFLLGLINGVLILSAPVFFFVSVWNGSYVSWQVF
                     FAWFFVLVGANVLLLLRFWQKRRLFSMRLKALAILGGGIVVWYWFFAPLYYFFSVNLN
                     ALQLTFAGFLYTWELPIVGGLFLVVALKRFRPIAAYEADKKKPEDPAHFYRAVRRYPT
                     EVAVLLFFLSVFAYGVGALQMQWFAFQPPLEQAKAFANGFVIAIFLSLFYYLFLDAFL
                     ERVRTKLKREYDIKGIKRQKAAQRVFFVILCTAVGSVGLVGLLVLQSWQVLEKESVLR
                     NMRDDAAQVALRLSQELQERQAGGTTQAQDNMSLFETLKQGERGQVVFLGAGETLPAE
                     SFSRRSREFVAQQPFGVLEDLKKEHKVVIFFEDATLSKKVVVVAFLSEFYSIFLPPLR
                     SFSIGAVLVLLLVTVMAALVSFALTDRVRRFSALVQQAERGEVDSLFPVPSDDELESL
                     SRSFIDYAKATKEIDRTKSEFVSLASHQLRTPLTITSWYAERLESKIKEQDYEAQLRY
                     LRAVQKANERMTKLLEALLNVSRIELGTISLQAERVDCVLVAESVLEEFVPQIREKDL
                     VFDKAYDKKGAYCLGDGEFLHMALENIVSNAVRYTPSGGEISLRVVAERNTVRVEIRD
                     TGCGIPREQQSKVFTKMFRADNAAQIDRMGSGLGLYITKWIVEQSGGKIWFESEEGRG
                     TIFYIQLPRYQEKGFPGKVGLWYNKNA"
     gene            complement(13965..14570)
                     /locus_tag="VFE94_00240"
     CDS             complement(13965..14570)
                     /locus_tag="VFE94_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49566.1"
                     /translation="MRGELPWYSKWRQMYAISNPIDTDNGYHPQNLFRLVTRSKWQNF
                     RQDVRFRITDVNMSQSPERNGWSGVLLFNRYLNGENLYYAGLRMDGYAVVKKKMGGTY
                     YTLSTDPVYVGSYDRIGNPNLIPQDRWIGLRSIVQNRSDGSVDIRVYVDKEDNGSWEL
                     VSSVTDDGRTYGGNALTQQGHAGIRTDFMDVQFDDYKAVSL"
BASE COUNT         3780 a         3915 c         3127 g         3848 t
ORIGIN      
        1 tacacatcca ttgtagaaga acaaacccaa ttgtcaagtg tttcctttac tttcttaact
       61 ttttacccat ttactcttta gattctaaac attactgcag aatcttgggg gggattgttt
      121 tggggaaaat gggcagctgg cccgtaatga taaaaatttt ctttacccct ttttctgcgc
      181 gcacctccct ttcgataaac tcttctatgg cgtgccagtt gtaggccaaa agcccaatgc
      241 ccaggttgta aatgccggtt atgcgaacca tgtgatacac ctcttgtttt ggctcgcatt
      301 tttcgatgag ctttttcaca gtttgctcgt ctgcctcaag aaatatctgc accgacatgg
      361 tataaaactg gtctgcctgg agctgggcct gaatggcaat ggtgcgttct cgcaggagct
      421 tgtccaccct gttttttacc gttaccgcgc tcaagttaat tgcttttccg atgtcggcaa
      481 acgtttctct tccgtttccc gcaagatgcg cgattatttt taagtccagc acgttcattt
      541 cgtcgaagtg ccaaatggac tctggcgaat acttccatac cgagaaatcc gcaagcccaa
      601 tgcggtcaat aaacgtagca aaccgtattt ctttaagttc gagctctttt ttacatagac
      661 gcaaagcttc catggaacgc ttgtacgcct ttcccgtaac aacgtcggcg tccacaataa
      721 gcactttccg gccgcgcagt tttgctgcct ccaggccggt cccgtcgtcc tccatggtgc
      781 ttatgatgat atcttttttc ttctctttag tcagccactc gtataaacct tgcgcatacc
      841 atacgccgtt gggttttaag taaataagag cgcctttatc tttgccaaag tacttttcaa
      901 tgtcttttcc cgccgtatca atgaattttt tgattgactc aaattctaaa aaactttgca
      961 tgggattgtt gctagaataa acttttttgg gtggtgaagg ttttgggttc accctctttg
     1021 aaaatctcta ttttcccgta ttcgccatca aagccaggct caatatgaac attcccctcc
     1081 cttacccttt ttattccctc ggcaatttct ggcagtgtgg cagactgcaa tccggcaata
     1141 tctgcgtcga gcaaaatcga gaactcgtct cccacagtat gaatgagctt ttcatactct
     1201 tctcttacgc gcttggatac ggtgccaacg cccaaagcgt ctgcaataat ttcttccaaa
     1261 ggaattaagc ttttgtaagg gatagcgcct tccggcctga acccctcggg cctgtctgcc
     1321 aactccgcca cccggtttag cacgcctatg gtaaggggcc tgccgcattt agggcaaatg
     1381 ttgttgtgct gctttgattc ttccggttta aagcttacgc cgcacaaccg gtggccgtcg
     1441 taatggtatt ttccttcttc gggaaaaaac tccacggtaa acaaaaattt ggaaggatct
     1501 tttttcttta atgcgtcagc tatcccctgg tagcttaact cggtatcgaa cacattagcc
     1561 tctctgccga ttttgggcgg cgaatgcgag tctgaattgc tgataaaagc aatgctgtcc
     1621 agctgcgata aacgccagtt catggcagga tcagacgaaa gccctgtctc aatagcatag
     1681 atgtatttgg tgtattcgtc aaagcattcc tggagcgagt taaagccgga cttggacccg
     1741 aatacggcaa accacggagt ccatgcatga gcggggatta ccatgcaatc aggagaagca
     1801 tcaagcgcaa tttttaccac ttcttttgca tcaatgccga taatgggcct gccgtctgcc
     1861 ttgagattcc cccgcacgcc caaatgcgca tttatctttg ctacggcctc aagcgaaggc
     1921 gcaaaaatca acaagtgcac ctttcgcact tgcccgcctt tggaataaat gcagcttaat
     1981 tccgtggtaa gaataaaacg cgtgccgagc ttgctttgtt tcaactggta aagcccctgt
     2041 tctacaggtt ctagtttttc ctgcaactcc ttaaaccaaa cggggtgcgt aaagtcgccc
     2101 gtacttatta ccttaatgcc cttgattttt gcccatgtat ccaaatgttc caagtccata
     2161 tcctgcgacg ttgccctcgc atacttggaa tgaatatgga aatcgccaac aaacttcatg
     2221 cattctttta taccacttga cggaggttct ggcgagggct aacgtaaata tatcttccct
     2281 ggaagaccgt tagcgaaaag caagctctta cgagaggaga aggggtaatg gatcctatcg
     2341 aaagatttcc gcaaaactgt atacaaatca gagaaattta tgcacacgtc ctcgcaaagg
     2401 agattgaccc ttgggaagat tgggtcctac aagctgtagc gagcgtgaag caacaggctg
     2461 aaacatacaa acttacagag cgcgacttcg ctaggttaat tcttgaccct tccttttact
     2521 ttctctcaca aaaatcgagg aaggccaaaa atgcctagcg cgaaggctcc atggtaatgt
     2581 cctaggtatg gacactcaca tagggccttc ttttttaccc taaaaatacc aaaggcggcc
     2641 cagctccaag ctccacaaca tcaacaaaag caaactcaag taagcaatac gaggcaaggc
     2701 aaaggccttt ttttgttgcc aaagcagcac caaaagccaa gctaaaagac caacatacaa
     2761 cacggaaagc agaaggcgcg ctattcctcc ccaatctctc gaggcagaca attgagagcc
     2821 tgccacctgc atacgcaaag gctcatttag ctctgtgtct ttaacttcta cacgcaccat
     2881 ggcaaggggt tctcgcggag gaatcggctc tatggaaatt tcgggttcct ggacaacaat
     2941 gttcgatacg gtaggagagc ttagtatttg gttgcttggc aagggccacc ccaaaaaagc
     3001 cactgccacg cgcgtttctc tgccatcaat tatcccttcg gcaaaaccaa tgcctacttc
     3061 acgaaaatcg gggttttgca attgcttggc gtggttggga gaatgcagcc atgcctgcac
     3121 caacactttg gaatccgcca catctatggc caggttctct gcggctcccg caaaagagta
     3181 tcctactcga tctaaccaaa cccatgcctc ctccccattg ggcccttggt gcgaaaaata
     3241 cccctgggcc accatatcct ctgctttcag ctgcgaagcc tgctggagcg ccgctgaaac
     3301 tcgcagaggc gaaagcccta tgtttcttcg ctcggcgtta atctcctcta caaccaaatg
     3361 ctggctaaga ctggcaagca gtaagctttt gtccgtattc caaacttcca gcaataacgc
     3421 caacacgaaa accgccccta ggccgtatat cgccagtgcg gttttcgcaa ataagacagt
     3481 gctcattact gtctattgta ctaaagaaga actccctgta ccactatacg ccctctgtct
     3541 ggggcaagag agatacacgt aatcatggtg aatactgctt cttctgttgg ttgcagcacc
     3601 tcaagagcgg tagcttccac cactcgggaa tccgaaactt cgtaggtata ggttttccct
     3661 tcccacacaa cttcaaaccg atctcctgct tgcagtttat cgagcaggta gaaagtttca
     3721 gaatagggtg ggagatactt gaatcggtgt gctcctacta cggtattgct tcctttatcg
     3781 ggcgtagagg ttccgggtat tctccatgct ccagactcca acgcagagtc gtcgtttcct
     3841 tccacaatcg gaatatgcac gccaatgctg ggaatcgtta agaagttttc agaacgagtt
     3901 tctgcgctct cttcttgcac tacgcccatt gcagtatgtg gcagcgtggg agcttgcacg
     3961 ataacgccta cagaatcttc tacttggggg tggttttctg ctcttgccac cacggtattc
     4021 atgtgtactc caagcacggc gtcctgtttt gcagtggcaa ccaatgtttc ctgccacact
     4081 tgccctggag ctaactgcgg aatcgtccac acgcgcagcc gttctgccaa gggactcgta
     4141 aagccagggg gaagcgtatc tatgacaagc acgttcaaag cagttgcctt gccggtattg
     4201 cgcagcgtaa tcgtaaagat tacctgttcg ccgcgctgca ccactggctt gtcaatggtc
     4261 ttctcaataa ccagcgcggg cttgaattct tcgccttgcg ttccaggcac tcgcacctca
     4321 atggtggctt tggcctgtaa gggctggtga ttgtctgctt ttacctcggc tgtgttagtg
     4381 taagacccca cctgaacctc ctgccctacc aaaacctcaa aggtaacggt ttgctgggca
     4441 ttgggaacca tacctcccaa ttgccacgtg cgattctgct ggctggtttc cttatgcaag
     4501 aatcctgcag gaagagtatc ggtcagcacg gtgtttattg ccgtaacagg accaatattg
     4561 ctcacttcaa tcgtgtagtt gactgttccc ccggggttgg taaacgactc tgcaatggac
     4621 ttttcaatcc gcaaggctgg tagcctgact gatcctcctc cccctccccc tccggatggc
     4681 ggcgttggtg aaggtgtagg tgtgggcgtt ggagtaggtg ttggagtcgg agtaggttca
     4741 ggttcaggtt cgggttctgc gcctagctct tcctcctttg cgtttccaaa gtcaatgtca
     4801 gaaatgtccg cgttggtaat tattacctcg tggcacccat tgttggctgt tgggaaagtt
     4861 tgattccatc cggccttctg cgtctcacac aatgtataag tcgcaggttc cagatcggta
     4921 aattcgtact ccccgttcgc gtcggttgta gtggaatcgt taaacgctgc tccatcggta
     4981 atggtaatgt cccagccttg aagaccgacc tcggttccat ctttcactcc atcctcgttc
     5041 tcgtcgttaa atttcatacc agagatggag aaggtggcgg gctgcacaac gggctttaag
     5101 gcgttaaatg ccaagcagta atacgttcta ccaagctcgg gtgagctgat aaaatcgtgg
     5161 ttgtcgtgct taaacacgtc cgtatggcac cacattctcg cggacggatc gctttgatcc
     5221 gtaccggtta cgtttggcca atcagaaaaa gcaatatact ctgcctgaag cacttcacgt
     5281 acccagattt tttcggttcc tttcgtatcg gtaatttcta ccgttgctac accattggca
     5341 ttggtagggc caaatgtctt ccattcgtta tcagcagttc ctgtcgctga agcttcgccc
     5401 acatggtcgc cgctctgctt gttttcgttg acattagcgt atccccactg gaactcccag
     5461 tcttttacca cagagcagcc ggggttgctg gctacaaaat cttgggcagt gtcttttgca
     5521 atgtccacgc ccgcatcact ccaatcagga aaatcagatg ggtcttcgca aactattttg
     5581 gaagcaacaa tagtgagctt ttttattaac tgctctggtt ctttcgcgtt tccaaagtcg
     5641 atgtcttcta catctttatc cgctaaagtt acctcgtggc agccattgtt gtccgtagga
     5701 aatgtttgat cccaatctgt ttgctgggtt tcgcaaactt catacacacc agcttccaga
     5761 tcggaaaact catagtttcc atcggcattt gtggttgtgg agtcagagaa agcgttcggg
     5821 ccttcgattt taatctccca gccttgcaaa cctccttctc cggcttcttt ggttgcgtct
     5881 ttgtccttat cctcaaactt catgccagag atagagaagg tatctgactc cttttcgcaa
     5941 aagtcagatt cctttccctg gtctccgtcg agcaggcgat cgccgtaacg cacgccgccg
     6001 gcaaacgact caataaggtc gccgtaaaac aagtcaatct ggtacgcgca ttccggaagg
     6061 tcaacgacaa gatcaacctc tgatcctgct gcgacaatag ctgtatcaga accaaacagc
     6121 tcttgattat ctatattctg gtcaaacatc ttgtacgacg ccaaccccac ctcataagcg
     6181 cacttttcag acctgttgcc tatgctggcg tttccggttc ctgtctggat actcccaaac
     6241 aagtcagctt ccggattcgg gacgcacgcg tcacctccac ccgtttctac aggctcctct
     6301 tcttcgtttt ccgtctcatg ggcatcttca tatccttttt cttccgccaa aacggtttgg
     6361 taggacacaa acccgttgcc tgcaggaata aagcttgctg aaaacaccat tcccgaaaaa
     6421 gcaaacaaca acaagaacgc atgcaccacg ctctttttat tggttagaaa tgatatatat
     6481 tccatatttt ttagttcccc cttaagtaag gtaattggtc cgacctacgc gccaaaacac
     6541 aaggcacacc aacgaaaacg cctctttaag agggattcaa cttataatga gagagttgtg
     6601 tttttaagga tccccgaatg tggggaaaag agctacagag aatacacttt gctagtatca
     6661 aaaacaaaaa aatgtattaa aaaaggacaa ctaaaaaggg agaaagaaca aaaaagagct
     6721 atgtttttta cccccggaga taggacttcc taccagatgt cctatctctc aagtactcta
     6781 tacgtagcag aatttaacag aactgtcaag aaacctgggc tctccctaca acggctgcac
     6841 ctccaaagat cgaaccaaaa gctggctttt tccaatctgc tcatcccaca tcttaaaccc
     6901 gacacctccg cgattaagat ccggttctaa aaacgtgctt tccaccacta tttcgccttc
     6961 caccaaacat tgaattgttc taccctgcac cctggcgctc acggtgaagt ccccctcttt
     7021 caaaggagtc accccgcgaa cccccttaat tacccgtttt tcccctttaa aaacctgctc
     7081 tgcgtgcaca aagtcgagcc caaagttaca ggccgcgttg ttgttcgcat cttgaaacct
     7141 tacccatata aatactcctg tttgagaagg aaggtttacc cttgccttca cttcgtaatc
     7201 ctcccaccct ccggttccgt cgaggactgc ggacgctcct gcctgtcccc cttctgccaa
     7261 aaataccagc cccttcccgg gctgcatatc aaaggagccc caattggaaa gccatgcatt
     7321 attttgtaag aaggattctt gatagggcaa ggatttagac atcccctgtt caaggatttg
     7381 aagtaaagcc tctcctgacc aattaggaag aacttctaat cttttcaccg tcaaaacgtc
     7441 ttcgcgggga tagttgaagg taaacccttc cccttcccac cactggtaaa acccgtggga
     7501 atagtgctcc tttgcgctga tcaacaaaat gcctttggcc aactcaaaat tggaatcggc
     7561 aaaaaggttg tgcccaaagt cgccaaaggg aaaagcaata gttgcaacag atatcccaag
     7621 agattcttct atttcctcct tggaaattcg caagtcctca taaattcttt cctggaactc
     7681 ctctggcgtt tctggtcttt cttcttctgg tacccacaac aagttgctgt aaaagtgccc
     7741 cgtttcgccg ttgggtcccg tagcgtaaag ctcgtgagca aactttccgt gggactggat
     7801 gtcccatctc cccgtgctct gcatatgctt cagttccttt tctgaaaggt aaaagtgcgt
     7861 tgccttccct ttttcgccga tcgactggtt cgtaattaca aacataactg cctcgtaatc
     7921 taacgcacgc aatatggggt ctacggggta ataactatcc ttgcggccgt catcgaaggt
     7981 aagcaaaaaa gacttttctg aaagctggag ttctcctttc ataaacgctt gaaactcttc
     8041 gatcgaaacg gtttcccagc cggcatcttt taaggccatc atttgctctt taaaatctct
     8101 cgtagttacg ttatcgcctt cggccttgcc cacaataccg tgatatacca agacaggaat
     8161 gcttaaagct tttccgcccc cctcgctctc caactcctta gactgggaaa tgggaaaaac
     8221 cgaagctaag aaactaccgc ctctatcccc agaaagcgta agttgatacc atgggtactt
     8281 gaacatcatt ataaaataac tttgctcaaa ataaatcgcg ttcgcggccc aaagagcccc
     8341 taccacaagc accaaagcaa atgccatatt cattgctata aaagatattc gtacattcat
     8401 aatggaaaag ttaccttgtc ccccaaccgc ggtttcgaat ggtagccaaa gcccaaaaca
     8461 gcacatacga aaggaacaat gcgttaagaa gcgcccaagg aataagatac ggccaatact
     8521 tatcttccgg gcgcaatatt ttgtaaaaaa tgataaagaa aataataacg gaggaagctc
     8581 ctataatgta atacagcggc gaaactccga agatgagagc gtcgaccata aaaaccttaa
     8641 ccgctataaa cggcgataaa aagtgagtga ggaacagcgg gaaaaagtag gtaacagcca
     8701 caaaggaatc tcgcttcatg ataaactttc cggcaaacag tgaatttaca aaccaccctt
     8761 tcttccagcg aatttgctgc tttacaaact ttttccaggt gtctggcaca atggtaaacg
     8821 accacgcttt atcggtatac actgatttat acccctggcg aatcacgcgg ttggtaaggg
     8881 ctctgtcgtc accccagttt gcaggcaccc ctaagaaact ctcgtttagc cattcgtcta
     8941 ttaccggcaa aacaacactt ttccggtacg ccgaggagca cccgctgcaa caaaagataa
     9001 gcgaaaacgc cgattctgct gcttttaaaa cgcggaaagc gacaaagtag tgtgccgtct
     9061 gcatcctggt gagaatgttc tgatccgcat tttccacata ggtatgtgcg cataccgccc
     9121 ccacttgcgg atttttgaaa taatcaacga gtttttgtat ggttccaggc tcgatataac
     9181 tatcactgtc cagctggaca accacttctc cccttgctcg cctaaaccct tctgccattc
     9241 catgccgctt cccccggttt tgttcccatg acacgaccgt taagttagga aaacggaact
     9301 gtaattttcc caaaatagcc cctgtgctat cagtacttcc gtcgttcact acaattactt
     9361 ccaccttttc tggcgggtat ctggcctcaa aacactttgt tacggttttt tctatagcgg
     9421 cctcctcatt cttgcaggga attacaaaac taattgcagg ctcataatgc atttgggcat
     9481 acggttctag cagtttttta tctgcttttt cataaaagag tgccgcccat atgcgggaaa
     9541 tccggaatat ggttacaaaa aaagtgtacc catacaaaaa tgggttatcg aaaaaattaa
     9601 aggcactgtt cctctttaaa agaacaatca aaaacaaaac cccgagcccc accgcgtaaa
     9661 ggaacagaag aatgaatagt tcgcggagaa taactttttt tgcctcccca gaaaaaatcc
     9721 ttctgctcgg agtaggcatt tgtttctttg ggatgattac cggacttggc atatgtttac
     9781 cttccgattc ctttgtatag tacttcgctt tgaaggaatg cttccttgga aagacagttc
     9841 cggccatcga ccaccacctg gactccgcgc gagtgcagaa attctggttc aaggttgcaa
     9901 aacgcagtgt gcgcggtagc aacgaccact gcccgggctc ctttgagcgc tttttccaag
     9961 ctggcagcgc tggatctctg caggacaaac gggtcgaatg tggctacgcg aagcccctgt
    10021 tgtttcaaac atttctcgat ttcgtaggaa gggctttccc ggtcgtcgtc gatatctggc
    10081 ttataggcaa gccctaaaag agcggtttta gtgcctttta cagggatatt ccgctccctg
    10141 agcccttctg tcagcaggtc ggcagcgtat ttgggcatac ccgcattgat agaccgcgca
    10201 agggacagaa aacggtgcaa aaagccattc tgctttgccg tatcaatcag atagtaaggg
    10261 tccaccggga tacaatgccc tcctaccccg catcccggat catgcgccat aaaggcaaag
    10321 ggtttggttg aggctccctg gagcacgttt gtaacgtcga tgcccatggt ggcaaaagac
    10381 atggcgagtt cgttcacaaa ggcaatatta atgtcgcgaa acgaattttc cactattttt
    10441 accgcttctg cttcttttac cgagcccatt tgggttacgg ggccttgcaa tatcgagcga
    10501 taaaaaccca ctgcctgctc tgtgctgcgc tcgtcgaccc cgcctaccac acgaggaatg
    10561 tattctatat tccactgggg atcgcccgga ttaatgcgtt ctgggcagtg cgcaaacgaa
    10621 aaatcctgcc cggcccgcaa gccagaaatc ttttcgagca aaggaataac aatattttca
    10681 gaactccccg ggcttaccgt ggattctaaa ataaccacct gctctttccg tagaaactcc
    10741 gccacgcgct tcgtagcttc ttttacggga gtaaagtcgg gggtgtggtc ttcgtgcact
    10801 ggtgttggga cgcaaatgat aataatgtcc gcctccctga tcttcgcaaa gttgtccgtt
    10861 gcctccatct tgcttttttt caaaaaagga actaacccgt cttcctgaaa aggcattatt
    10921 cttgcagaaa gaagcctaat tctctctttt gcaatatcga tgccaattac ccggtacccc
    10981 tttcgctcag ccaaaagcgc aaggggcaac cccacgtacc ccaaacccac caccgcgact
    11041 gtcccgcctg acggcggcaa aattttcctc ttttttgaag cattcatacc gtactaataa
    11101 gacgtagggg cagccctttc gttacgggca atacatgcaa aatgaaaacc tccccttttc
    11161 aaagaggagg ctcgcaaaaa agcctagaag acctgtttgg attgtagaga gttagtgatt
    11221 gggggagtca cgaaccatat gagtaatgga aagaggttac gcttatatat taccgacctt
    11281 ttttcttcct cttccctctt taatcggtac tttctttaac acccaaacaa gaaaaaggtg
    11341 tcgagcgctc gacacctttt tcgcaaaaat ctttggccga tttatacaat tcccagtttt
    11401 tctttggcgc ggcctaccac atctttgaga ccccacctgg tttttaccat atattcatat
    11461 acatcgtgcc ttagagcttc catggtcttc tcaaagtctc cttcggcatt ggttaaaatc
    11521 aaaacccggg cctgttttcc ccactcatcc tcccgaagct tctttaacat ggacatgccg
    11581 tccaaacggg gcatcaccaa gtccaaaata ataaggtcgg gctgctcggc aagagcaagt
    11641 tccaacccct ctaacccgtc tcttgccgca acaacgtcta cctcttgctc ggtaaattct
    11701 tccaccaaaa tcccccgcaa gaggtcttcg tcctcaataa cgagaacttt tttcctttgg
    11761 tatggttgat tcatgcgttt ttattatacc acagacccac ttttccggga aaaccctttt
    11821 cctggtatcg cggaagctga atataaaaga ttgtaccacg tccttcctcg gattcaaacc
    11881 atatctttcc ccccgactgt tccacaatcc acttggttat atacaggcca aggccgcttc
    11941 ccatacggtc gatttgagcc gcgttatccg cgcggaacat cttcgtgaat acctttgact
    12001 gctgctctcg ggggattcca caccccgtgt cgcgaatttc cacacgcaca gtattcctct
    12061 ctgcaacaac tcttagcgaa atttccccgc ccgaaggcgt ataccgcact gcattggaga
    12121 cgatattttc caaagccatg tgcagaaact caccgtctcc caaacaatac gctcctttct
    12181 tgtcgtaggc cttgtcaaag acaaggtctt tctccctgat ttgcgggacg aactcttcca
    12241 gtacgctttc tgccacgagg acgcaatcaa ccctttctgc ttggagcgaa atcgtaccca
    12301 gttcaatgcg cgatacgttg agcagcgcct ctaaaagctt ggtcatgcgc tcatttgcct
    12361 tttgcaccgc ccgaagatac cgcagctgtg cttcgtaatc ctgttctttt atcttgcttt
    12421 ccaatctttc cgcataccaa ctggtaatag tgagcggggt tctcaactgg tgggaagcaa
    12481 gagacacaaa ctcgcttttc gtgcggtcga tttctttggt agccttggca tagtcaataa
    12541 aagaacggga aagactttca agctcgtcgt cggaaggcac agggaaaaga gaatcgactt
    12601 cgcccctttc tgcttgctgc accaaggcag aaaaccttcg aacgcgatcg gtgagcgcaa
    12661 agcttactaa agctgccata accgtaacta acaataacac aagcacagct ccgatgctaa
    12721 aagaacgaag cggaggaagg aaaatgctgt aaaactccga gagaaaagcc actaccacca
    12781 cttttttaga gagagtggcg tcctcaaaaa atataaccac cttatgctct ttttttaagt
    12841 cctcgagaac cccaaaaggc tgctgggcca caaattccct ggagcgcctg gaaaaagatt
    12901 cggcaggcag cgtctctcct gccccgagaa aaacaacctg cccccgctca ccctgtttga
    12961 gcgtttcaaa caaagacata ttgtcttgcg cctgcgttgt tccgcctgcc tggcgctctt
    13021 gaagctcctg ggacaggcgt aaggcaacct gcgctgcgtc atctcgcata ttacgcagca
    13081 cgctctcttt ctctaaaacc tgccaggatt ggagcaccaa aagccccacg agccccacgc
    13141 tgccaaccgc ggtgcaaaga atcacaaaaa acacccgttg ggctgctttt tgccttttga
    13201 tgcccttaat gtcgtattcc ctttttagct tggtgcgcac tcgctccaag aacgcatcga
    13261 gaaaaaggta gtaaaaaaga gaaaggaata tggcgataac aaatccgttg gcaaaggctt
    13321 tcgcctgctc taaaggcggc tggaacgcaa accattgcat ttgcagcgcg cctaccccat
    13381 acgcaaaaac cgataggaaa aaaagcaata cagcgacttc cgtaggatat cggcgcacgg
    13441 cacggtaaaa gtgagcggga tcttcgggtt tctttttgtc cgcctcatag gccgcaatgg
    13501 gtctgaaacg cttgagcgcg actacaagga aaagtcctcc cacgatagga agttcccatg
    13561 tatagagaaa cccggcaaac gtgagctgaa gcgcattaag attcacgctg aaaaagtagt
    13621 agaggggcgc aaaaaaccaa taccaaacca cgattcctcc cccaagaatc gccagcgctt
    13681 tcaagcgcat gctaaacaaa cggcgctttt gccaaaatcg caaaagcaac aacacattgg
    13741 cgcccacaag gacaaaaaac caggcaaaaa atacctgcca ggaaacatag gaaccattcc
    13801 ataccgaaac gaaaaagaac acaggggcac tcaagataag gacgccattt atcaacccta
    13861 gtaaaaacaa agggtgttgg gttatttttt tgaaaatcac agtcttttat taaaccacaa
    13921 aaaagcttcc ggggctagcc ccggaagcga ccaaaccttc acccttaaag cgataccgct
    13981 ttgtaatcgt cgaactgcac gtccatgaaa tcagtccgaa tgcccgcgtg cccttgctgc
    14041 gtgagagcat tccccccgta ggttctccca tcgtccgtga cagacgatac gagctcccaa
    14101 ctgccattgt cctctttatc cacatatacg cgaatgtcaa cgcttccatc ggaacgattc
    14161 tgcactatgc ttcgtaaacc aatccaacgg tcttggggga taagattggg gttgccaatc
    14221 cggtcatagg aacctacata tacaggatca gtggacaacg tatagtatgt tccccccatt
    14281 ttctttttga ctacggcata cccgtccata cgcagacccg catagtaaag attttctccg
    14341 ttaagatacc tgttaaaaag cagcactccc gaccatccgt tgcgctcggg actttgagac
    14401 atattcacat cagtgatgcg aaaacggaca tcctgcctaa agttctgcca tttgctgcgg
    14461 gtaaccaaac ggaaaaggtt ctgggggtgg tagccgttgt ctgtatcaat aggattggaa
    14521 atcgcataca tctggcgcca tttggaatac cacggaagct ctcctctcac cgttcttcct
    14581 ataccgtttt cgaggataag atgcgcaccc gaattaaccc accaatacgg gtgcgcatct
    14641 tatcctcgaa aacggtatag gaagaacggt
//
LOCUS       DASRUP010000006        17234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1422669, whole genome shotgun sequence.
ACCESSION   DASRUP010000006 DASRUP010000000
VERSION     DASRUP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17234
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422669"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFE94_00245"
     CDS             complement(<1..230)
                     /locus_tag="VFE94_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HZX49567.1"
                     /translation="MNYNLAENYLEESKIEIKKIRDGLGQGMAELGATKKDMVVLVAD
                     TMESSRAHFFGEKFPERLIQVGVAEQNLTGIAA"
     gene            complement(220..1053)
                     /locus_tag="VFE94_00250"
     CDS             complement(220..1053)
                     /locus_tag="VFE94_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZX49568.1"
                     /translation="MQQLSIKELQKKANEIRQDIIKMLLEAGSGHSGGPLGMADIFTA
                     LYFRVLKHDPKKPKWADRDRLVLSNGHIVPVRYVTMMHAGYIDKKHLPTLRKIGSILE
                     GHPSVKAIPALETSSGPLGEGLSQACGMAMAAKMDGNQNKYHIWCLTSDGEHEEGMTW
                     EAAMFAAKYRLSNLTCIIDRNNIQIDGNTEDIMPLEPLRDKYESFGWHVIDMNGNDME
                     EIVTTLEEAKAITEKPVCIIAHTIPGKGVSFMEYDYKWHGVTPDKEQAKEALKELQHE
                     L"
     gene            complement(1078..2451)
                     /locus_tag="VFE94_00255"
     CDS             complement(1078..2451)
                     /locus_tag="VFE94_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49569.1"
                     /translation="MNPFHDLKPEIKLFLIVAVASVFIAVAGILLLRESSPTPSVSEG
                     KALVYGKAVALSDVQSIPGGPLVEGIIGAIPEDHLTSFAKEARQPNTALISQATIYSY
                     FVNFSLLDEQGEFQFELDPGSYVLCYFSPTTPSEAMRLEACARANLERGERLTIKVEK
                     MFGTSITGKGIETVPLTDIFPLEHDSFNASADTSTWQTYRNDEFGFEFTHPSWGETTG
                     AFGPYFSGGSDLVLTLVSGQILGNHEGVSVHSNIEILPKLSNISLKERLETKYNIKDG
                     QKGNIDTAYQQNQELSEYYWTVLKPSQKPGTAIEYPKGKIVYNEYSLNGIPIFELGDP
                     GGSFTYMTFFLPQNKDYIYVLTLELSGAGNGESVVPPDSYFIAQYKEMVSTFRFVEEP
                     EINWNWELLGCGGQTPCSYRVSSSQLSGVYMCAGKYNPLSGQGEVTPTPSTDPRTTTD
                     FTCQLEE"
     gene            complement(2478..3557)
                     /locus_tag="VFE94_00260"
     CDS             complement(2478..3557)
                     /locus_tag="VFE94_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49570.1"
                     /translation="MMQNLKPEIKLFVIVAIVAVVIAVGSIFLLRAGVSNQVTHTPSP
                     QPQAISNEQSAIDTSDWQTYRNDEFGFEVKYPKGFAIADGDQSFIRRDGGSFGINISD
                     KNNGTAALHVSVIETNLSPREWLDEHYTAIPANYNDVYAGHYSTPQDTIVNGVSALRF
                     SLFFTSGSLQSTIIQKDSNVLYHIAAGSDSRGSFPQETYNQILSTFRFVDKKQTTVVT
                     PAQTVSVATDKQEYKSGEIVRIRVLNGLQEAILIPPVSGYSNPIFAIQRKLEDGTWEN
                     VYPPPPPIAPPALDEYQKINSGNTLEYIWEKTEITSSHTFRAPISYVSENHAKQLGYP
                     VKKGIYPGREDIVSIYSNEFRIIIE"
     gene            complement(3662..4723)
                     /locus_tag="VFE94_00265"
     CDS             complement(3662..4723)
                     /locus_tag="VFE94_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49571.1"
                     /translation="MSFLDDFKSEIKGIILLVAAIVITVVFVGGISFLREGAPIGEQP
                     LPGESGLNTSGWQTYRNEEYGFEIKHPPGTTFGPVFDGTFFMLEGFEGGNIIVGTQSP
                     YPRDYNNPDGEVRLETQDVSFKDIQAKLRVVYVDNVPAEIAMYDFQDIPHASWGESAE
                     LLIFVGDQSKGQMLEDILDTFRFVEDSEFEQLLQQYAGFQELFATVVQANTSLAPSSF
                     ERLEEKPITVQWVLDSDGSDLENPDWVVSPNGSKALLVSSGEEPDSTFQIYNRNNQRK
                     VEVLKSCGTPCSYRGASWLDNETFVFVQSQEYHPAGQTRCTVDTACTFVVAMSVYNLA
                     NNTEIVYQSTELNERPALQ"
     gene            complement(4785..5345)
                     /locus_tag="VFE94_00270"
     CDS             complement(4785..5345)
                     /locus_tag="VFE94_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZX49572.1"
                     /translation="MKIILPLLAIVSLFLPFLAQANAFALVFSPVEDTYSAGDTFVVE
                     ILVDTQNQDVNAVAAYVDYPSDLLSVVSMDMKDAPFMFIVEQNYGAGKIAISGGTPTP
                     GFNGVYRVATIEFQAIEPGDATLSFTGDSAVLLDSDNTNILQGQPQATVRIANANIGT
                     APSVQAETPQYRSPLETLLAFLKGLL"
     gene            complement(5354..5983)
                     /locus_tag="VFE94_00275"
     CDS             complement(5354..5983)
                     /locus_tag="VFE94_00275"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZX49573.1"
                     /translation="MQKVVPAILTADPAELREGLKVLRNQTKWVQIDIMDGRFVPNTS
                     INLFELGEAYQFFNLEIHLMVQNPEKYFEDCKAIGAKRVIFHAEATGNIAAVLEEMEK
                     YPFQRAVAINPETPVSIIAPYTDKLDAVLIMSVHPGFQAQEFIPGVLSKIAEIRRLKE
                     DIIVGLDGGINEKNIKGVFEAGADYAGVGSAVMKTEDPAQALTNLEQML"
     gene            complement(5985..6434)
                     /locus_tag="VFE94_00280"
     CDS             complement(5985..6434)
                     /locus_tag="VFE94_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HZX49574.1"
                     /translation="MIYLASDHAGLDLKEKLKKYIEQELGEEVEDMGAFAMNPDDDYP
                     DFVIPAAEKVGKDPEHNLAIVLGGSGQGEAIAANKVKGVRAALYYGGPLDIVKLSRQH
                     NNANILSLGARFVSLEEAKEAVKLWLETSFQEGRHERRVNKISEFEE"
     gene            6482..6622
                     /locus_tag="VFE94_00285"
     CDS             6482..6622
                     /locus_tag="VFE94_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49575.1"
                     /translation="MGWFFVILAILFPEQMRTAFGCVVGLLIALLFLAVVCVVAAGLG
                     TI"
     gene            complement(6652..7203)
                     /locus_tag="VFE94_00290"
     CDS             complement(6652..7203)
                     /locus_tag="VFE94_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX49576.1"
                     /translation="MEVAHTRRVVNYRIFLSAHILVSVAMGLFGILFIREFGGSLYSF
                     GYSVAVMMGAGALASLFAGRLSDRFGRKRLLVASAVAFALLVILYTLINSLVELFVLQ
                     SMYGIIGSIEATAGSAFLGDATEKASRGKDIGKLYATTGIASACALAAGGHIFSWFGF
                     EMSFYGVAAFFALSATLLLFIKE"
     gene            complement(7248..7694)
                     /locus_tag="VFE94_00295"
     CDS             complement(7248..7694)
                     /locus_tag="VFE94_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HZX49577.1"
                     /translation="MNKSFFLILGGVLIAGAALGFAFMGGDPAQEVPIQEQEISAQEQ
                     EIVAHTSIDYGNGEIQSFANEVIEQGASALDLLDKLGQEKGIAIEKKDFPGLGEFVEA
                     INGVHGSGDSYWQFWINGEYAQVGAGQYELKEGDEVLWKLTNEQPQ"
     regulatory      complement(7800..7942)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8982..9248)
                     /locus_tag="VFE94_00300"
     CDS             complement(8982..9248)
                     /locus_tag="VFE94_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49578.1"
                     /translation="MAWQDIVFSIGSWVFVFALLPSVFSKDKPAFSSSITTATVLTVF
                     VFTYASLGLWLTAVSAGLLTLTWCVLAIQKFLMNQKVKKVMPQA"
     gene            complement(9313..10320)
                     /locus_tag="VFE94_00305"
     CDS             complement(9313..10320)
                     /locus_tag="VFE94_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49579.1"
                     /translation="MFGMELYELFNFTRQAGLALAGATSLWALVFLWRARKSEGEKKK
                     ALEWAANRLLLAFSVAFGAAAFSWFVLWNIWPVLAHEGITLFPSGTEYRSAFLLTAPL
                     YVGWILFTLGVLVCRKFRKEVFARFLLWFYGAQFLVVSVLISLYAWTGSALGGEQLFY
                     YFHGFHSIFTLGTVLTLDFLFLSTRTALSLQRHLFPFFPAISKIIFIGFGLDFLSVFL
                     VFDTAVEFSARFFMAQTVVGILIINGAFMAGPLTRKVLNGLAEGRLLAGTWRYIAGVA
                     GAVSITSWMTITFIDFFPRTAFSYTQLMGAYASVILFGIVANFFIARLDERHLASMAK
                     P"
     gene            complement(10334..10489)
                     /locus_tag="VFE94_00310"
     CDS             complement(10334..10489)
                     /locus_tag="VFE94_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49580.1"
                     /translation="MGNHISELLFALAGALFLASAFVVYQAQDFTLWHFAKLFYVVAL
                     LLFVKYS"
     gene            complement(10493..11569)
                     /locus_tag="VFE94_00315"
     CDS             complement(10493..11569)
                     /locus_tag="VFE94_00315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZX49581.1"
                     /translation="MIKAFVVLSLAVAGIVGGAYLWKSFNDAPPEQSQEAPGMEQEQS
                     PVVFPIEEYAQRRTVNAFGEEGQVTLEGFHVADDIEYTDADAQVPVFAIADGIVQRGD
                     WVRGYGGVMVIQHDIEEKTYTAIYGHLDQGSIQIADGEQVSRGQQVAYLGEGETEETD
                     GERTHLHFALYEGTEQRIQGYEPSPENLENWLNPHDFFAFYGLDTQSPARVYNPQEDL
                     GGSDFALEFAIPEGWEVEYDDTAHLISLFELSGEGPARERAQVTFTYFDAESFLTLST
                     VTIHEVADTQVGQQNYTAKQYDIEKKQGVSAFQGQPAWRQQRHTAIDFRGKEGRTRYY
                     SVAKNPELDQAVFQSIVDSITIVR"
     gene            complement(11587..12153)
                     /locus_tag="VFE94_00320"
     CDS             complement(11587..12153)
                     /locus_tag="VFE94_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49582.1"
                     /translation="MILDFIEIKTIYLIFHLFGIALGAGGAFASDLIFFKSIKDGQLT
                     KTEFGFMELGSKMIWAGLGVLVISGTLLFFLNPEGYMASSKFQLKMTVVAIIIANGIV
                     LHLNFIPKLKAATKIGDINVAEFVRQNPFILVSGVVSMVSWSSAIILGALRGIPYSYW
                     LILAIYLAIILCGIAAAFLLKPRMFPKQ"
     gene            complement(12150..13406)
                     /locus_tag="VFE94_00325"
     CDS             complement(12150..13406)
                     /locus_tag="VFE94_00325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX49583.1"
                     /translation="MLKKVSLDQKVRAKDFLKFAPMAQQQRILEYVQALKGKKIVHVN
                     ATGQGGGVAEILQSFIPYLNVFGLRAEWYAIDADQPGKRFFEITDKIRNGLQGQAVEI
                     KKEEWDVYENANREITKELESIACDILVVHDWQPAYAGFYLARSTPKVFVSHADTSSA
                     YKPVADQIMEAMRGYDAMIFSNKDFVYPEFAEKTNIHVIAPAIDPLSLKQSTVPQKDA
                     RAYLVPFGVPGTGRFIVQVSRFDNWKNPQGVVEAFRIVQESHADVYLALVGIEEAQDN
                     PDSKKVYEEVKGMVGDNPHISLFFSAKGIPHIAEFTMMAQNAADIVVQNSIKEGFGLT
                     VTEAMWKEKPVVGGPASGVRLQITHGKNGFIAENTKDLAMYMDFLLSHPKERARVGKA
                     AKESVRKRFLMPRFVLDHLKVYKELI"
     gene            complement(13412..15052)
                     /locus_tag="VFE94_00330"
     CDS             complement(13412..15052)
                     /locus_tag="VFE94_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX49584.1"
                     /translation="MTLRDELQKLVKGEVLDDEETLKKYSRDYSIFEVRPQVVVFPKN
                     AEDVKNVVLFVNKYPKQKLSVTARSGGTDMGGGPLNESIILDFSKHMNKLLKIGKDYA
                     LVQPGMFYRDFEKASLKKGLLFPSFPASRDICAIGGIVSNNSGGENNLVYGKTENYVL
                     EYQMVLVDGKEYTFRPIDRAELSRKLKLKGFEGDIYRKLFKLVSSNAELLAKAKPRVS
                     KNSAGYYLWNVWNGSTFNIPKLLVGAQGTLGITTQVKLKLIKPKEHSALVVIFMKDVK
                     ALGQLVDIVLEKKPETFEAYDDQTLKLALRFLPDFVKLLGAKNMLSLAWQFLPEFKMM
                     LGGLPKLILLAEFTGDSKEEVQKTAKETQLLIRRFGFPTRVAKTQKESEKYFTIRRKS
                     FSLLHSHAQGLFAAPFIDDVVVKPEHLPEFLPQLHAILKPYRKQMVYTIAGHAGDGNF
                     HVIPLVDLSKPDVRAMIPEVMQKVHKLVVRYRGSITGEHNDGLIRTPYLKDMYGPRVV
                     KLFEEVKDIFDPDNIFNPGKKVGGSLAFAIKHMITHKE"
     gene            complement(15052..16167)
                     /locus_tag="VFE94_00335"
     CDS             complement(15052..16167)
                     /locus_tag="VFE94_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49585.1"
                     /translation="MKETIYFRKWFCVLILLVLGVSVVIPSSLEARSSSRDARVQASF
                     VQMKWIAELIYNEGNGGYESLQCASQGDYGREMQILCEDVEAQFDRKPVIHSGNEEYC
                     AYAVLPSQDGYVCIDSTQGENQTLIITYINPGNAGYCNGTTFVCPTEVGTPSPLSVQE
                     RTLETSKFALLFVAGIFFVFVWWRSIKRRVRETPSRGVFEELKGEIASKKLYFSALVL
                     FLLIPAVSWGIFVALYSEFLFDIPFPVHMALVSFFEVVSVYFFFASLLAPLVILALSL
                     VPIQRSWKNPNEGVCWESYLAALCLSVTLFVLSYFSAGFERGAVELISFMLLISGLGV
                     GLCISCIISKRKINKLMGIVLLCLFAVLLSIFITNLA"
     gene            16302..16844
                     /gene="cas2"
                     /locus_tag="VFE94_00340"
     CDS             16302..16844
                     /gene="cas2"
                     /locus_tag="VFE94_00340"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HZX49586.1"
                     /translation="MEMGPTKKKILLLLLGGVLLGFAYSPYGQRKIYREISKEWEKID
                     REKFRKNISALHRSKYVTVKQLPDGSYTYVLSAKGKSKALTYQFQTIRIPKQKWDKKW
                     RLVAFDIPEDYKKARDALRRKLRQLGFQELQKSVFILPYECHKEIEFIVEFFGVHKYV
                     RYGVLESIDNSAKLRKSFGI"
     gene            16905..>17234
                     /gene="truB"
                     /locus_tag="VFE94_00345"
     CDS             16905..>17234
                     /gene="truB"
                     /locus_tag="VFE94_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720347.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZX49587.1"
                     /translation="MILNIDKPAGVTSHDVVNQVRKITGESRVGHAGTLDPFATGVLV
                     VAVGREDTKKLGPINENSEKEYEALLELGKTSSTGDPDGTIQETLDPEKVSAITRVQI
                     EHVLQTYT"
BASE COUNT         4642 a         4353 c         3733 g         4506 t
ORIGIN      
        1 gccgctatgc cggttaagtt ctgctctgcc acacccacct gtattaggcg ctcggggaat
       61 ttctcaccaa aaaaatgcgc cctggaagat tccatggtat ctgccaccag caccaccatg
      121 tcttttttgg tagcgcccag ctctgccata ccctgcccca acccatcgcg gattttcttt
      181 atctcgattt ttgactcctc caaataattt tccgctaggt tataattcat gctgaagttc
      241 tttaagcgct tcttttgcct gttctttgtc gggggttacg ccgtgccact tgtagtcgta
      301 ttccataaag ctcacgcctt tgcccggaat agtgtgggca ataatgcaca cgggcttttc
      361 ggtaattgct tttgcttctt ctaacgtggt tacaatttct tccatgtcgt tgccgttcat
      421 atctattacg tgccagccaa aactttcgta tttatcgcgc aagggttcca acggcataat
      481 gtcttcggtg ttgccgtcta tctggatatt gttgcgatcg ataatgcacg taaggttgct
      541 taagcgatat ttggcggcaa acatggcggc ttcccacgtc atcccctctt cgtgctcgcc
      601 gtctgacgtt aaacaccata tgtgatattt gttctggttg ccgtccattt tagcggccat
      661 ggccatgccg cacgcctgcg acaggccctc gcccaacggc ccggagctgg tttccaaagc
      721 gggaattgct tttaccgagg ggtggccttc caaaatcgaa ccgattttgc gcaaggttgg
      781 caaatgcttt ttgtctatgt agcctgcgtg catcatggtt acatagcgca cgggtacaat
      841 atggccgttg gacaacacca gccggtcgcg atcagcccat ttgggctttt tggggtcgtg
      901 ttttaacacg cggaaataca aggcagtaaa aatatcggcc atgcccaaag gcccgcccga
      961 atgcccggat cccgcctcca acagcatttt gatgatatcc tgccgtatct cgttcgcctt
     1021 tttttgtagc tcttttatag acaactgctg catatatgta ttgtaccaaa tttagcttta
     1081 ttcctctagt tgacaggtaa aatctgtggt tgtgcgggga tctgtactag gcgttggagt
     1141 tacctcgcct tgcccgctta aagggttgta ttttccggcg cacatatata cgcccgacaa
     1201 ttgactggag ctcactcggt acgaacaggg agtttggcct ccgcatccca aaagttccca
     1261 attccaattg atttctggct cctccacaaa ccgaaacgtg gaaaccatct ctttgtattg
     1321 tgctataaaa tagctgtctg gcggcaccac gctttctcca tttccagctc ccgaaagctc
     1381 caatgttaat acgtaaatat agtctttatt ttgaggtagg aaaaaagtca tatacgtaaa
     1441 tgaaccccca ggatcgccaa gctcaaaaat cggaattcca tttaacgagt attcgttgta
     1501 aacaatttta cctttgggat actcgatggc agtgcccggc ttttgactag gttttaaaac
     1561 agtccagtag tattcagaca attcttgatt ttgttgataa gcagtgtcaa tgttgccctt
     1621 ttgaccgtct ttaatgttat atttagtttc aagacgttct tttagagata tatttgagag
     1681 cttgggtagg atttcaatat tagaatggac actaactccc tcatgatttc ctagaatttg
     1741 accgctaact aacgttagaa ctaaatcact tcctccggaa aaataaggtc cgaaggcacc
     1801 tgtagtttca ccccacgacg gatgcgtgaa ttcaaaccca aactcatcat tgcgataggt
     1861 ttgccaggtg gaagtgtcgg ccgaggcatt aaaggaatca tgttcgaggg gaaatatgtc
     1921 agtaagcggt actgtttcta tgccctttcc agtgatactc gttccaaaca tcttttcaac
     1981 cttgattgta agccgttccc ctcgctcaag attagccctg gcacacgcct cgagtcgcat
     2041 agcttcagaa ggagtcgtag gagaaaagta gcataacaca tatgatccag gatctagctc
     2101 aaactgaaat tcgccctgct catccaataa agaaaaattc acaaaatatg aatagatggt
     2161 ggcttggctt atgagagcag tgtttggttg tcttgcttct tttgcgaaag aagtaaggtg
     2221 gtcttctgga attgctccga taattccctc aacaagcggc ccgcccggta tgctttgtac
     2281 atcacttaag gcaaccgcct taccgtaaac aagtgcctta ccctcggaaa ctgaaggggt
     2341 gggcgagctt tcgcgcaaca gcaaaatccc ggcgacggcg ataaacaccg acgcgacggc
     2401 gacgataaga aataacttta tttcgggttt gaggtcgtgg aaagggttca taaagttact
     2461 tgctccacaa gatttgatta ctcgataata attctaaact cgtttgagta aatgcttacg
     2521 atatcttccc ttccagggta gatgcctttt ttcacaggat agcctaactg tttcgcgtga
     2581 ttctctgaca cataactaat gggtgctctg aaggtatggc tgctagtaat ttctgttttt
     2641 tcccaaatat actccagtgt attaccggaa tttatctttt ggtactcatc aagagcgggt
     2701 ggcgcaatcg gaggaggcgg tggataaaca ttctcccacg ttccatcttc caattttctt
     2761 tggatagcaa aaataggatt actatacccg cttacaggag ggataagtat agcttcttgc
     2821 aaaccattca atacccttat tcttacaatt tctccactct tatactcctg tttatccgtg
     2881 gcaaccgata cggtttgtgc cggcgtaaca acggtagtct gctttttatc cacaaaccga
     2941 aacgtggata gaatttggtt ataggtttct tgcggaaatg atccccgact atcgctccca
     3001 gcggcaatgt ggtaaagaac gtttgagtct ttttgaataa tagtgctttg tagcgacccg
     3061 cttgtgaaga acaaactaaa tcgcagagca gataccccat taacaatagt atcttgagga
     3121 gttgaataat gtccagcata aacatcgtta taatttgcgg gaatagccgt atagtgttca
     3181 tctagccatt ctcgaggtga taagttggtt tctatcacgc tcacgtgtaa tgctgcagta
     3241 ccattatttt tatctgaaat attgatacca aaactcccac catctctcct tataaatgat
     3301 tggtcgccgt cggcaatagc aaaacctttt gggtacttta cctcaaaccc aaactcgtcg
     3361 ttgcggtagg tttgccagtc ggaggtgtcg attgctgatt gttcgttgct gattgcttgc
     3421 ggctgcggcg atggcgtgtg agtaacctgg ttactcacac cagctcgcag aaggaaaata
     3481 ctcccgacgg caataacgac ggcgacgatt gccacgatga cgaatagctt gatttcgggt
     3541 ttgaggtttt gcatcataag taaagaaagt taacttattg taggaattac attattgtac
     3601 tatttctggc aaaaaataaa acccacaagc agttatatgc ttgcgggggg acggctacat
     3661 ttcattgtag cgcgggcctt tcgtttagtt cggtagattg ataaacaatt tctgtgttgt
     3721 ttgccaggtt gtatacggac atggctacca caaaagtgca tgctgtatct acggtgcagc
     3781 gcgtttggcc tgccgggtgg tattcctggc tttgcacgaa cacaaacgtt tcgttatcca
     3841 gccacgacgc gccccggtaa gagcacggcg ttccgcaaga ttttaacact tctaccttcc
     3901 tctggttatt ccggttatat atctggaaag tagagtctgg ttcttcgccc gaactaacca
     3961 aaagcgcttt gcttccgttt ggggaaacca cccagtctgg attttccaga tcgctgccat
     4021 cagagtctaa cacccactgc accgttattg gcttttcctc caggcgctca aaactgcttg
     4081 gcgcaagact ggtgtttgcc tgaactactg tggcaaaaag ttcctggaaa cccgcatact
     4141 gttggagtaa ttgctcaaac tccgaatctt ccacaaagcg aaaagtgtcg agaatgtctt
     4201 cgagcatctg accctttgac tggtcgccca cgaaaataag aagttccgca gattcgcccc
     4261 aactggcgtg cggaatatcc tggaagtcat acatagcaat ttcggcgggc acgttatcta
     4321 cataaacaac gcgaagcttg gcttgtatat ccttaaatga cacgtcctgg gtttccaagc
     4381 ggacttctcc atcggggtta ttgtaatctc tgggatatgg gctttgcgtt cccacaataa
     4441 tattgccgcc ttcaaaccct tcgagcataa aaaacgtgcc gtcgaacacg ggaccaaaag
     4501 ttgtccccgg cggatgcttg atttcaaacc cgtattcctc gttgcgatac gtttgccagc
     4561 cagacgtatt aaggccagac tcgcctggca acggttgctc gccgataggc gcgccttcac
     4621 gcaaaaacga aatcccgccc acaaaaacca cagtaataac aattgccgca acgagtaata
     4681 taattccttt gatttcggat ttgaaatcgt caagaaaact cattacaata ttatatcaat
     4741 ttctttgcca aacacaaacc ccctctttcg agggggggcg gtttttagag taatcctttt
     4801 aaaaaagcaa gcaatgtttc aagcggactc cggtactggg gcgtttctgc ttgtacggaa
     4861 ggcgccgtgc ctatgtttgc gttggcaatg cgcaccgttg cctggggctg gccttgcaaa
     4921 atattcgtat tgtcgctgtc tagaagtacg gcggaatcac cggtaaaaga tagcgttgcg
     4981 tctccaggct caattgcctg aaattctata gttgctacgc ggtacactcc gttaaaaccc
     5041 ggggttggcg tgcctcccga aatcgcaatc ttgcctgcgc cgtagttctg ttcaacaata
     5101 aacataaaag gcgcatcttt catatccatc gacaccacag aaagcaagtc ggaaggataa
     5161 tccacataag ccgccaccgc gtttacatcc tggttttggg tatcaacgag aatttcgaca
     5221 acaaatgtgt ctcctgcgct ataggtatct tctaccggcg aaaaaaccaa agcaaaagcg
     5281 tttgcctgtg ccaaaaaggg caaaaataag cttacaatag ctaaaagggg aagaattatt
     5341 ttcataaccc ctattatagc atttgctcca aatttgtcaa ggcctgggca ggatcctctg
     5401 ttttcataac cgcgctgccc acgccagcgt aatctgcgcc cgcctcaaac acgcctttga
     5461 tgtttttctc gttaataccc ccgtccaggc ctacaatgat atcctctttt aacctgcgga
     5521 tttccgcaat tttggaaaga acgcctggta taaattcctg cgcctggaac ccgggatgta
     5581 cgctcataat aagcacggcg tccagtttgt ctgtgtaggg ggcaattatg gaaaccgggg
     5641 tttcgggatt tatggctaca gcccgctgga aaggatactt ttccatttcc tccaaaacgg
     5701 cagcgatatt gccggtggct tcggcatgaa aaataacccg ctttgcccca atggctttgc
     5761 agtcttcaaa gtatttttcg gggttttgca ccattaaatg gatttccagg ttaaaaaact
     5821 ggtatgcttc tcccagctcg aaaagattta tcgacgtgtt ggggacgaaa cgaccgtcca
     5881 taatatctat ttgcacccat tttgtttgat tgcgcaaaac tttaagaccc tcccgaagct
     5941 ctgcgggatc tgccgtaaga attgccggaa ctaccttttg cattttactc ctcaaactcg
     6001 ctaattttgt taactctccg ctcgtgcctg ccttcttgga acgaagtttc cagccaaagt
     6061 tttaccgcct ctttcgcttc ctcaagcgaa acaaatctgg ctcccagcga tagtatgtta
     6121 gcattattgt gctgtcgcga aagctttacg atatccaggg ggccgccgta atacagtgcc
     6181 gcccgaactc cctttacttt attggccgca attgcttccc cttgcccgga acccccgagc
     6241 acgattgcta ggttgtgttc cgggtctttc cccacttttt ctgccgccgg aattacaaag
     6301 tcggggtagt cgtcgtccgg gttcattgca aaagcgccca tgtcttccac ctcttcccct
     6361 agctcttgct ctatatattt tttgagtttc tctttcaggt cgaggcctgc gtggtcactt
     6421 gccaaataga tcatataggt atagtataca gccaaaattc ttgtgagcaa aggagggcaa
     6481 aatgggatgg ttttttgtta tcctagccat tcttttccct gaacagatgc gcacagcctt
     6541 tggctgtgtt gtaggcctgc ttatcgcgct gctatttctt gcggttgtct gtgttgttgc
     6601 tgcaggcttg ggaacaatat aaatccaccg aaccaaagcc cggtggatgt tttattcctt
     6661 tataaacaaa aggagcgtgg ctgaaagagc aaaaaacgcc gcaaccccgt agaaggacat
     6721 ctcaaagccg aaccagctaa agatatgtcc gccagcagcc agtgcgcaag ctgaggcaat
     6781 gccagtggtg gcatacagtt tgcctatatc ctttccgcga gatgctttct ctgttgcatc
     6841 ccccaggaag gctgatcctg ccgtcgcctc tatggaacct ataatcccgt acatggactg
     6901 caacacaaac aactcaacta aagagttaat taaggtgtag aggattacaa ggagcgcaaa
     6961 ggcaacggcc gaagctacga gaagacgctt tcgtccaaac ctgtcggaaa gccttcctgc
     7021 aaacagcgaa gctaacgcgc cagcccccat cataacggca accgaatatc cgaaggagta
     7081 aagcgaaccg ccgaattccc ggataaacaa aatgccgaac aaacccatgg caaccgaaac
     7141 cagaatatga gcgcttaaaa aaatccgata gtttactact cgccgggtat gagcaacctc
     7201 catccctaac cccctattgc gttgttaaag ttcgttggaa ctacttttta ctgcggttgc
     7261 tcgttggtga gcttccacag aacctcgtcg ccttccttaa gctcatactg gccggcgccc
     7321 acctgcgcgt actcgccgtt tatccaaaac tgccagtaag agtcgcctga accatgcacg
     7381 ccgtttatcg cctcaacaaa ctcccccagc ccgggaaaat ctttcttttc aatggcaatg
     7441 cctttttcct ggcccagttt gtcgagcaag tcgagggcgc tagccccttg ttctattacc
     7501 tcgttggcga acgattggat ttcgccgttt ccgtaatcaa tgcttgtatg cgcaacaatt
     7561 tcttgttctt gggccgaaat ctcctgctct tggattggaa cttcttgcgc gggatccccc
     7621 cccataaacg caaatcctag cgccgcgccg gcaattaaaa ctccgcccag aatgagaaaa
     7681 aatgatttgt tcatataact aaaaatctcc caggagtagg agaaacgcgc ttgccaaaat
     7741 ttaatactta aattcgactg cgagaatccg ccccctccct cgaggaacga taattcgttt
     7801 gtttattatg ggataatcgc tagtcgaccg gacttatccg ttgcgccatt tggcggacgg
     7861 cttcaccgtt gcggggcagc gtgggcgttt cacccacttc ctctaacgaa tatcattgtc
     7921 gacgaactta ctgtataatc ctttttacct cgccgtaatg acagcgtcaa gcataccaca
     7981 tctagtcccc tgtggatttc tttttgcaaa ccgagcacga ccctttgttc tttatacacg
     8041 cgatcgcgcg cataaagaac aaccaacctt atcgttttaa ctctgctata gcataactaa
     8101 aacgatagtt ttcaatgcat ccaaccgcgg ggaaaagaaa agccccggct gcttgtagac
     8161 ggggctcaaa taaggtgcgg gtgtagagga ttattggttt tcggggcaaa gtcgacagtg
     8221 cattggagcc ctgcagatat tactaagttc ctggatacgc ttctccagcc attctcgaag
     8281 atcggccatc gaaagcgtag atggggcgta tctctcaagc gaacctcgta cactcggtga
     8341 aagctcatca atagtttcaa gaggcttccc ttcctcttcg aacagaaaga ttatttttcg
     8401 cgcaagacat aactcccccc actccatagg tacttctaaa ggaacctctt gtcttgttgt
     8461 cattactcaa ctccttttga acacggttta cttcacccta ccccatctac ggggaaaaga
     8521 aaagccccgg ctgctataaa ccagggctcc ggctaaccct aagggtcgcg agaacgtttg
     8581 ctaaaggaac ggtgtcccca tatatccggg gataagaacg aattcaccag acgatgtgcg
     8641 gctaagatac tttcacgtcg ggcacgcccg gcggtaaaac cttttacaaa cgctattgag
     8701 gaattcccgt cgaccaactc gcaaaaggta ctaatgggca acgtaaccgt accctccgtc
     8761 tgcgaggtaa caatcccaac cacaaaccct gcggcttcgt ttgtcgaggg acgccagtcg
     8821 cgaactatct cgtggtccga ccctggcaaa ttgtgggctt caacgtaagc ccttgcattg
     8881 ttggattccg gaaatacctg gactacgatt tttgccggcg ctaccatgcc ttttcctcct
     8941 cattggaccg tgcctatata ttagctgtaa gaccgtagaa atcaagcttg cggcataacc
     9001 ttttttactt tttgattcat gaggaatttt tgaattgcga gcacacacca agtaagagta
     9061 agcaggcctg cagaaacagc agtcagccac aagccaaggc ttgcgtatgt aaatacaaaa
     9121 acagtaagca ctgtcgccgt agttatactg ctcgagaacg ccggtttgtc cttgctaaac
     9181 accgagggca agagcgcaaa aacaaaaacc catgacccga ttgaaaatac aatatcctgc
     9241 cacgccataa gaagattgta acacaaaacc cccaaaccca ggtaaataca agagatgttt
     9301 aaatgagata catcaaggct tggccatgga tgcaaggtgg cgttcgtcga gccgtgcgat
     9361 aaaaaagttc gcaacaatcc caaacaaaat aaccgaagcg tacgccccca taagctgggt
     9421 gtaagaaaac gccgtgcgcg ggaaaaaatc aataaacgta atcgtcatcc acgacgtaat
     9481 ggaaacggcg cccgcaacgc ccgcaatgta acgccatgtg cccgcgagca ggcggccttc
     9541 tgcaaggccg ttaagcactt tccgggtaag cggacctgcc ataaacgcgc cgtttataat
     9601 aagaatgccc accacggttt gcgccataaa gaagcgcgcg gaaaactcta ctgccgtgtc
     9661 gaataccaaa aacacgctca aaaagtccag gccaaaccca ataaagataa ttttgctaat
     9721 agccggaaaa aacggaaaca aatgccgctg gagcgaaagc gcagtacgcg tggaaagaaa
     9781 caaaaaatcg agtgtaagca ccgtgcccag cgtaaaaata gaatggaacc cgtggaaata
     9841 gtagaataac tgctcgccgc ccaaagcgct gccggtccac gcgtataacg aaataaggac
     9901 agacacaaca agaaactgcg cgccgtagaa ccagagcaaa aagcgcgcga aaacttcctt
     9961 tcgaaatttg cggcacacaa gcacgcccag ggtaaacaaa atccacccca cgtatagggg
    10021 ggcggttagc aaaaacgcgc tgcggtactc tgtgcccgaa gggaacagcg taatgccctc
    10081 atgcgccaaa accggccaga tattccacag cacaaaccac gaaaaagcag ccgcgccaaa
    10141 cgcaaccgaa aacgccagca aaagccggtt tgccgcccac tcaagcgcct ttttcttctc
    10201 tccctcactt ttcctggcac gccaaagaaa tacaagcgcc cagagcgaag tagcccccgc
    10261 taaagccaag ccggcctggc gcgtaaagtt aaacaattcg tataattcca ttccaaacat
    10321 agaaaaatct agactagctg tattttacaa acaacagaag tgccaccacg taaaaaagtt
    10381 tggcgaaatg ccaaagcgta aagtcctgcg cctggtacac cacaaaagcg ctcgcaagga
    10441 ataaagcacc tgccagcgcg aacagcaatt cgcttatatg attccccata tgctatcgca
    10501 caattgttat gctgtcgact atgctttgaa aaaccgcttg gtcgagttcg ggatttttcg
    10561 caacactgta gtagcgggta cggccttctt tgccgcgaaa atcaatcgca gtgtgccttt
    10621 gttgccgcca cgcgggctgg ccctggaacg cgcttacgcc ctgcttcttt tcgatatcgt
    10681 actgctttgc cgtgtaattc tgctgcccca cctgggtatc cgccacttcg tgaatggtta
    10741 ccgtcgaaag cgtaagaaag ctctcggcat caaaataggt aaaagtaacc tgcgcgcgtt
    10801 ctcgtgccgg cccttcgccc gacaactcaa acaagcttat cagatgcgca gtatcgtcgt
    10861 attccacctc ccatccctcg ggtatggcaa actccaaggc aaagtcgctc cctcccaaat
    10921 cttcctgcgg gttatacacg cgagcagggc tttgagtatc taaaccgtaa aaggcaaaga
    10981 aatcgtgggg attaagccag ttttccagat tttctggcga aggctcatac ccttgaatgc
    11041 gctgctctgt cccctcgtag agagcaaaat gaagatgcgt gcgctcgccg tcggtttctt
    11101 cggtttcgcc ctctcctaaa taagccactt gctggcccct ggaaacctgc tctccgtctg
    11161 caatctgaat ggagccctga tcaagatggc cataaatcgc agtgtatgtt ttctcttcaa
    11221 tatcatgctg gatgaccatc acgcctccat acccccgcac ccaatccccc cgttgcacaa
    11281 taccgtctgc aatggcaaac accggtactt gcgcgtctgc atcggtgtat tcaatatcgt
    11341 ctgccacatg aaaaccctcg agcgtaacct gaccttcttc gccaaacgcg tttactgttc
    11401 ttctttgggc atactcctct ataggaaaaa caaccggcga ttgttcctgc tccattcccg
    11461 gggcttcttg cgactgctcg ggtggagcgt cgttaaacga tttccaaaga taggctcctc
    11521 cgacaattcc agcaacggca agagacaaaa caacaaaagc ttttatcata attcagtttt
    11581 ttagtcttat tgtttgggaa acatgcgggg ttttagaagg aaagctgcgg caatgccgca
    11641 caaaattatg gcaaggtata tggcaagaat aagccagtag ctatacggaa tgcctcgcaa
    11701 agcgcccaga ataatggcag aagaccacga aaccatggac actactccac ttaccagtat
    11761 aaaaggattt tgccggacaa actcggctac atttatatcg cctattttgg tggcagcttt
    11821 cagtttggga ataaagttta ggtgcagcac aatgccgttt gcaataataa tagcaaccac
    11881 ggtcatttta agctgaaact tactggaagc catatagcct tccgggttta ggaaaaacag
    11941 caaagtgccg gaaataacca aaacaccaag tcctgcccat atcattttac ttcccagctc
    12001 cataaaacca aattccgttt tcgtaagctg gccgtctttt atggacttga aaaatataag
    12061 gtcgctggca aaagcgccgc cagccccaag ggcaatgcca aacaaatgaa aaataaggta
    12121 tatggtttta atttcaataa aatccagtat catataagct ctttatacac ttttaagtgg
    12181 tcgagcacaa agcgcggcat taaaaagcgc ttgcgcacgc tttcttttgc cgcttttccc
    12241 acgcgcgccc tttcttttgg gtgcgaaagc aaaaagtcca tatacattgc caggtctttt
    12301 gtgttttcgg cgataaatcc gtttttgcca tgcgtaattt gcaggcgcac tcccgaagcc
    12361 ggcccgccca ccaccggctt ttctttccac atggcttccg taaccgttaa gccaaaccct
    12421 tctttgattg aattttgcac cactatgtca gcggcgtttt gagccatcat ggtaaactcc
    12481 gcgatatgtg gaataccctt ggcagaaaag aaaagagaaa tgtgtgggtt gtcgcccacc
    12541 atgcctttta cctcctcata tactttcttt gaatcggggt tgtcttgcgc ctcctcgatt
    12601 cccacaagcg ccaggtaaac atctgcgtgg ctttcttgca ctatgcgaaa cgcttcaaca
    12661 acgccttgcg ggtttttcca gttatcaaac ctggaaacct ggacaataaa ccgacctgtt
    12721 cccggtacgc caaaaggaac aagatacgcg cgcgcgtctt tttgcggcac tgtgctttgt
    12781 ttaagcgata agggatcaat ggccggcgct attacatgaa tgtttgtttt ctccgcaaac
    12841 tccggataca cgaaatcttt gttgctaaaa atcatggcgt cgtatcctct catcgcttcc
    12901 ataatttggt cggccaccgg tttgtaggca ctcgaggtgt ccgcgtggct cacaaacact
    12961 ttgggagtag aacgcgcgag ataaaatccc gcatacgccg gttgccaatc gtgaaccaca
    13021 agaatatcgc acgctatgct ttctaactct ttggtaattt cacgattcgc gttttcgtat
    13081 acatcccatt cttccttttt tatttccacc gcctgaccct gcaacccgtt tcgtatttta
    13141 tcggtaatct caaaaaaacg ttttcccggc tggtcagcgt caatggcata ccactccgcc
    13201 cgcagtccga atacgttcaa gtacggaatg aaactttgaa gaatttccgc cacgcccccg
    13261 ccctgacccg tggcgtttac gtgcacaatt tttttgccct tgagagcttg tacgtactcc
    13321 agtatgcgct gctgctgcgc cattggagca aacttgagaa aatcttttgc gcgaaccttt
    13381 tggtcgaggg aaactttttt cagcatacac gctattcctt atgcgtaatc atatgcttaa
    13441 tagcgaaagc caaactcccg cctactttct tgcctggatt aaaaatattg tccgggtcga
    13501 aaatatcttt tacttcttca aacagtttaa ccactcgggg gccgtacata tctttgagat
    13561 acggagtgcg aatcagccca tcgttgtgct cgcccgtaat agaccccctg tatcttacta
    13621 ccagcttatg gactttttgc atgacttcgg gaatcatggc ccttacatcg ggtttcgaaa
    13681 gatccactaa gggaataaca tgaaagtttc cgtcgcccgc gtgcccggca atcgtgtaca
    13741 ccatttgctt tcgataaggc ttgagaatcg catgtaactg cggcagaaat tcgggcaagt
    13801 gctccggttt tacgaccacg tcgtcgataa agggcgcggc aaacagcccc tgggcatggc
    13861 tgtgcagcaa gctaaaactt ttgcgccgaa tggtaaaata tttttcgctt tctttttggg
    13921 tttttgccac gcgcgtggga aagccaaagc gccttatgag cagctgcgtt tcctttgcgg
    13981 ttttttgcac ttcctctttg gaatcgcccg taaattcggc aagcaatatg agcttgggca
    14041 atccgcccaa catcattttg aactcgggca aaaactgcca tgctaaagaa agcatgtttt
    14101 ttgcgcccaa aagctttacg aaatcgggca aaaaccgaag agcaagcttt aatgtttggt
    14161 cgtcgtaggc ttcaaaagtt tccggtttct tttcgagcac tatatcaaca agctgcccca
    14221 aagcctttac atctttcatg aaaataacca ctagggcgga atgctctttt ggcttaataa
    14281 gcttgagttt tacctgcgtg gtaatgccca aggtgccttg cgcgcccacc agaagtttgg
    14341 gaatgttaaa cgtgctccca ttccacacgt tccacaagta ataccctgcg gaattcttgg
    14401 aaacccttgg tttcgctttc gccaaaagct ctgcgttgct gcttacgagc ttaaaaagtt
    14461 tccggtagat gtcgccctca aagcccttca gttttaactt gcgggaaagc tcggccctgt
    14521 cgatgggacg aaacgtgtac tctttgccgt cgaccagaac catttgatac tccagcacgt
    14581 agttttccgt tttcccgtat accaagttgt tctctccccc cgaattattc gagacaattc
    14641 cgcctattgc gcaaatgtcg cgagaggcgg gaaacgaagg aaacaaaagc ccctttttga
    14701 ggcttgcctt ttcaaaatcg cggtagaaca ttccgggctg cactaaggca taatcttttc
    14761 cgattttcag taacttgttc atgtgctttg aaaagtcgag aatgatagat tcgttcagag
    14821 ggccgcctcc catgtcggtt ccgccagaac gcgcggtaac cgagagtttt tgcttggggt
    14881 atttgtttac aaaaagcaca acattcttta cgtcttctgc atttttggga aatacgacta
    14941 cctgcgggcg cacttcaaaa atgctgtaat cccggctgta tttttttaac gtttcctcgt
    15001 cgtccagtac ctcgcctttc accaattttt gcagctcatc tcgtaaagtc attatgctaa
    15061 attagtaata aagattgata ataaaacggc aaagagacaa agcaaaacaa tgcccataag
    15121 tttgttaatc tttctcttgc ttatgataca gctaatacaa agccctactc ctaaaccaga
    15181 aatcaaaagc atgaaactta tcagttcgac agctcccctt tcaaaaccag cactaaaata
    15241 actaagaacg aaaagggtta cggataagca aagtgcagca agatagcttt cccaacagac
    15301 cccttcattc ggatttttcc aagatcgttg tatcggcaca agactaagag ccaagattac
    15361 aagaggcgcg agcaaagaag cgaagaaaaa gtatacgcta actacttcaa aaaaggagac
    15421 tagcgccata tgaacaggaa aaggaatatc aaaaagaaac tcagagtata aagcaacaaa
    15481 gatgccccag gaaacggcag ggatgagcaa gaaaagcact aaagcactaa aatagagctt
    15541 ttttgaagct atctcaccct tcaactcttc aaaaacgcct cttgatggcg tttctcgtac
    15601 tctccttttt atcgaacgcc accaaacaaa cacgaaaaat attccggcga caaacaataa
    15661 ggcaaatttt gaggtttcaa gagttcgttc ctgtacagat aacggagaag gtgtaccaac
    15721 ttcagttgga cagacaaacg ttgtgccatt gcaatagcca gcattcccag gatttatgta
    15781 tgtaattata agtgtctgat tttcaccttg tgtgctatca atacagacat agccatcctg
    15841 cgaaggtaaa actgcatagg cgcaatactc ttcattgccg ctatgaataa ctggcttcct
    15901 atcaaactgc gcctctacat cttcacagag aatttgcatc tcacggccgt agtcaccttg
    15961 agaagcacac tgaagacttt cgtagccgcc attcccctcg ttataaatca actcggctat
    16021 ccacttcatc tgcacaaaac ttgcttgcac tcgcgcatct cgactactac tccttgcctc
    16081 caaagaacta gggataacta cgctcacgcc caacactagg agaatcaaca cacaaaacca
    16141 tttcctgaaa tatatcgttt ccttcattat tccattgtac cttatcgcca ccaccatgca
    16201 attgtggaga actacaaaaa agtaaaacgg agtattggta cttgaattac gcgatcgcgt
    16261 atttacttga ccagtcagta tggctactct acaataaaaa tatggaaatg ggtccgacga
    16321 aaaagaaaat tctgcttttg ctcttaggcg gtgtattact tggctttgct tactccccct
    16381 atggacaaag gaagatttac agagagataa gcaaggagtg ggaaaaaatt gaccgagaaa
    16441 aattccggaa aaacatttcc gcgcttcata gatcaaagta cgtaacagta aagcaactac
    16501 ccgatggaag ttatacctat gtactttccg ccaaggggaa gtcaaaagcg cttacgtacc
    16561 agttccaaac aataaggata cccaaacaga agtgggataa aaaatggagg ctggtagcat
    16621 tcgatatccc cgaagattac aagaaagcaa gagatgcgct ccgcagaaag ctccgacaac
    16681 taggatttca agaattacag aagagcgttt ttatacttcc ttacgaatgc cacaaagaaa
    16741 tagagtttat cgtagagttt tttggcgtac acaaatatgt gcgatatgga gttttagaat
    16801 cgatagacaa tagcgctaaa ttgagaaagt catttggtat ctaaaatacg cgatcgcgta
    16861 ctttaaatac cagaattatt actatctgtt gttgcctgtt atttatgata cttaatatcg
    16921 acaaaccggc aggggttaca tctcacgatg tggtaaacca ggtgcgaaag atcacgggtg
    16981 agtcgcgcgt ggggcacgcg ggcaccctag accctttcgc taccggagtt ttagtagtgg
    17041 ccgtgggcag agaggacaca aaaaagcttg gcccaataaa cgaaaattca gaaaaagaat
    17101 atgaagcctt actggaactc ggaaaaacga gcagtacggg agatcccgat ggaactattc
    17161 aagaaaccct tgatcctgaa aaagtttccg ccattacgcg tgtacaaatt gaacacgtgt
    17221 tgcaaacgta cacc
//
LOCUS       DASRUP010000007        14704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1896480, whole genome shotgun sequence.
ACCESSION   DASRUP010000007 DASRUP010000000
VERSION     DASRUP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896480"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..629
                     /gene="recR"
                     /locus_tag="VFE94_00350"
     CDS             12..629
                     /gene="recR"
                     /locus_tag="VFE94_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZX49588.1"
                     /translation="MQKLIEALSRFPTVGPRTAGRFVFYLLKAPDQEIEELAQAIRNL
                     KKEIKLCGFCFNPYQATEDENKSGLCGICRDQSRDHAIICVVEKESDLEALERTKNYK
                     GLYFILGEAMNMLKKDGIKELRRKELEERLESPEKFGLSGVKFQEVIIATNPTTEGEA
                     TALYVERFLAQMGVKTTRLARGLPVGGELEYADEETLRSALEGRR"
     gene            626..1273
                     /locus_tag="VFE94_00355"
     CDS             626..1273
                     /locus_tag="VFE94_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49589.1"
                     /translation="MIPFYENIFPAALFWFLVAMLFAAIEIEIEGKHGWAEKTSTWFR
                     TKGLAARLYGLAMGGKPLTGYHLFMFFLPLLVVHSHFVMGVPWSVEAELLALALYFVW
                     MPTWDFLWFVLNPYYGVKKFKKQKVWWHARSHWLFNITPLDYLFGWGLSVVLAVAATL
                     VADDLRLVFWHLHFMVWFFFFTVLTILLVAPLYKRWHAKMRQRDDREASGIFHAE"
     gene            1347..2045
                     /locus_tag="VFE94_00360"
     CDS             1347..2045
                     /locus_tag="VFE94_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49590.1"
                     /translation="MTESNALYNDLLSRARQALPFVSSHPREPGRAEAAILLAEESGQ
                     EHDLNIAKRLVDELAEPEERLMGLEDIAKITQQSEDRLAALQADDAYEGTWQIDKEFT
                     PGNISFSRSILVWFSPYPITQEAISSERNEARAELLQSHVSQLVDQGRLEDALFVAES
                     LRTDALIEDGEARERFWGRCVGAVAVGYARAGNFSQANDFAELIVFLEDEYIELLVEI
                     ARTLRTTKEDHPNG"
     gene            complement(2060..2380)
                     /gene="rplU"
                     /locus_tag="VFE94_00365"
     CDS             complement(2060..2380)
                     /gene="rplU"
                     /locus_tag="VFE94_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZX49591.1"
                     /translation="MAEMAVIKTGGKQYVVTPGKKLKIEKLPVQEGSDVVFEEVLLLE
                     GQKGLEIGAPLVQGAKVQGKILKQAKAKKVVIFKFKAKKREKKKRGHRQPYSEVQITS
                     IEAK"
     gene            complement(2416..3453)
                     /locus_tag="VFE94_00370"
     CDS             complement(2416..3453)
                     /locus_tag="VFE94_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZX49592.1"
                     /translation="MQDTIYILIIAGLVVAVFFFWRSGQKQQGAGGQEIFMIQGQIAE
                     LRQAVDDKLGTSATMFQQQYSESAKLIQAVTQELTRVSEGQKQVMNITEQLKSLQDIL
                     KNPKQRGVLGEYYLESLLKNVMPPGSYQMQYGFPNGTIVDAAVFVKDKVIPVDSKFSL
                     ENYNRIVEATDPAEKERLQKVFVQDLKNRIVETAKYIQPEQGTTDFAFMFIPHEAIYY
                     DLLVNTIGAVGDDTENLVQRAAGRYRVIIVSPTSFLAYLQTVLQGLKAMQIEESAIKI
                     IKRVEDLGRHLKTYEEYHNKLGNSLGTSVNTYNAAGKELGKIDKDVLRITEQAPGLDP
                     LQLEKPNRDED"
     gene            complement(3478..4467)
                     /locus_tag="VFE94_00375"
     CDS             complement(3478..4467)
                     /locus_tag="VFE94_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HZX49593.1"
                     /translation="MLFHFLLFLFSGFLLLWSSNWLVSSVTHIARYLRWKEFVIAFFV
                     MAVVASFPNLFVGIIAAFNDAPELSFGDVVGNSVVDLTLVAALAVFFGRGLRGDGPLV
                     QRSSLITAAVAILPLLLILDHQLGRGDAVVLLLTFFFYSIWLFSKRGEYTHVFDHTDP
                     AQTKIPPIARFRAFFINILKALVGILILLASAQGVVNAVLFFAEAFDLPLAFIGILVL
                     GFGSALPEIYFTIAAARHANSSIILGDLMGAVIVMSTMVLGIVALIQPIRIEDFSPFA
                     LARVFLVVSALLFLIFLRTDRRITAKEGVFLLLLYIAFVAGEVFIRLYGNLPE"
     gene            complement(4474..7125)
                     /locus_tag="VFE94_00380"
     CDS             complement(4474..7125)
                     /locus_tag="VFE94_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZX49594.1"
                     /translation="MSLEDIFRTNWHAVSAREAANILESDLERGLSEQTVARRREFFG
                     YNKLPEEKPPSKILIFSKQFKNPFIIILVAAGLVTLFFREYTDTIVIFGTVLINTVIG
                     YAQENRATNALAKLKQILKHKAWVVRAAQEKEIPQQDVVPGDILVLNPGSKVPADGRL
                     LSSWNLRVNEAVLTGEWIAASKKNTVLDEKTPLADRDNMVYMGSVVEEGNGKAAVTAI
                     GLQTELGKISSLVSSVQETRTPYQEKLARFSWLIGIGVSLLAAIIFLQGILLGEELFH
                     MFELAVAIAVSAIPEGLPIAMTVVLAIGMQRILKAKGLVRSLPAAETLGSTTVIATDK
                     TLTLTEGKMQVEEIVPLRFADREQTLLAGMLANEAFVENPAEMLEKQIIRGRPTDRAL
                     LQAGMEAGLSKTKLEENLPVILRIPFESATRYVASFHRTPEGANLYVSGAPETLLSLS
                     QVSKDEKAQALATLAELTQKGLRVVSCATRQIPQDQLAGKSQEQLSHFFKSQIKDLTF
                     IGFVALKDPIRKGVKEAIEAAKGAGIQTVIVTGDHALTARAIAAELGLPAQHDNIMEG
                     RHLDALGERELQKLLPKISVFARVEPSHKLRIIEAWQEKGAIIAMTGDGVNDAPALKK
                     ANIGLALGSGTDVAKETADLVLLGDNFSIIPAAIKEGRVILDNIRKIITFMVSGTFTE
                     TILIGSTLVLGAPFLPVTALQILWINLVEGTFPSIALTLEKAEKDIMRRRPPKPNTSL
                     LTGEMKTLIFVISVITDFLLIGLFYWLLGNSTYSPQHIQTVLFVGLGIDSLLYVFSCK
                     SLRKNIWQYNPFSNLWLVASVLFSFILLFAVIYIPLLQRLFETRPLNLFDWTLLAALG
                     IINIVLIEAGKWVFIQRDKKADSNGEI"
     gene            complement(7208..8113)
                     /locus_tag="VFE94_00385"
     CDS             complement(7208..8113)
                     /locus_tag="VFE94_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49595.1"
                     /translation="MKRFLQYILGSLILISVASTGTQMVFAQEERVQTHKERVEELRA
                     KAESARERALESASRKREERQEEKIHIKLQENTQSANPDTENFNAKVEQFRQAREQFH
                     QNPSSVAMRKTMLLHVVDAFRAHTQLLKEQVDRFPIIHEDLKNSLLAEIEADQAALEE
                     FRLEIQAAKTIEELKALAEQLGKHRKEVTEQKVRKLMLLAHIGVFEYRILDVAAARAA
                     KIEEALDALEARGQSTTESQSLLAAANAKIQGATNALEELKETIIANDIDRVQLRDAQ
                     KALRDATHAVREAYQLFRDIARETR"
     gene            complement(8121..8699)
                     /locus_tag="VFE94_00390"
     CDS             complement(8121..8699)
                     /locus_tag="VFE94_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49596.1"
                     /translation="MTPEDSEQLLLSVLELKDQGKSTAEILAFFPEHQDEVKQTLAAL
                     EALQRQHKLQPRKDLLQTILWQLPPVTKNEATRSSLTERIFSPWKITFAASAFALVFL
                     LFWGYTQPAPQGLALADINEEAEGIEQLLTQSDWYFEEQQTMEDLNDFDAVALEAEAA
                     KEEQDIDSATELDAFFEEEMKVEEVGDALEDF"
     gene            complement(8696..9256)
                     /locus_tag="VFE94_00395"
     CDS             complement(8696..9256)
                     /locus_tag="VFE94_00395"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX49597.1"
                     /translation="MNNPEEFEELVQQAKSGNENAFSHLYEYYLTPIFRFVYFRVRSQ
                     EDAEDLTQHVFLKAWSALPEFQEEGRSFSAWLYRIARNSTIDYWRKRKDIPLDPTSKT
                     LHNKESKTENPARLAEKGDTARMIREALHILNEDQQTIIELKFIEELSNKEVAAVTGK
                     SEGAIRQVQCRALKILRSHFQENNLS"
     gene            complement(9319..9391)
                     /locus_tag="VFE94_00400"
     tRNA            complement(9319..9391)
                     /locus_tag="VFE94_00400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9355..9357),aa:Glu,seq:ctc)
     gene            complement(9422..11110)
                     /locus_tag="VFE94_00405"
     CDS             complement(9422..11110)
                     /locus_tag="VFE94_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZX49598.1"
                     /translation="MRNHSHTQEGWRRTLLLLVIVCMGIGLLSRLAYLQIFEYGFYKA
                     LAQGQQNLSSYGRGVRGDIFIQDRLGNLHLLATNQKLPYVFASPPEVQDAQDEAQKLA
                     DILSIPSEEILPKLQRKESLYEPIKKRITDEEKTRMAELNLRGIYLGEENVRSYPLGS
                     LASHVAGFVNTEGKGQYGVEGYYNDVLGGKEGLLKSSWNPATYFLSNRETVEDGSDIV
                     LTLDYNIQAVAEELLQKVRQELNVEKASIIVMDPADGKLLALANIPSFDPNEYSKVED
                     LEVFQNSAIQKLFEPGSIFKPITMAGALNEGAVTPSTTYVDYGIVQIGGSKVYNYDQR
                     TWGERSMTEVLEYSINTGVVFAERKLGHANFLDYLKEFGIFQATNIGLAGEVFSRNTE
                     FQKGYEINFATAAFGQGIEMTPLQIVRAFSAIANSGVPAQPHIVLEEKPKEGALPILS
                     SRTSSQLTAMLTSVVENGYAKTAQIPGYYVAGKTGTAQISWAALGVAKGGYSDQTIQS
                     FIGYAPAFNPRFLILVKLDNPAAKTAEYSAVPIFQDLAKYIIDYYQIPPDYEVE"
     gene            complement(11128..11427)
                     /locus_tag="VFE94_00410"
     CDS             complement(11128..11427)
                     /locus_tag="VFE94_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49599.1"
                     /translation="MSTLYHSKLFWFLPLVIAGLLVGMYVFQLNALTALAYHIADQEQ
                     LATQLKQDVTQLEIRARHTFSLNDLEELAQNMQFEKITNISYLKIPTGGVAANQP"
     gene            complement(11405..12286)
                     /gene="rsmH"
                     /locus_tag="VFE94_00415"
     CDS             complement(11405..12286)
                     /gene="rsmH"
                     /locus_tag="VFE94_00415"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZX49600.1"
                     /translation="MHTPVLLEKVLELLNPQPNQNFIDCTAGAGGHTFAILELAAPKG
                     KVLAIDWDPNAISLLKPQNRLLAREGNYADIAEIARQEKFHPVHGILFDLGFSLNQIE
                     KSQRGFSFMRNEPLDMRYNQNNPDTAEKILNYYSRTQLEHTFKEYGEEQYAKQIAAEI
                     IEVRAQKPIRTTKQLVEVIERATPKSYRHKKIHAATKTFQALRIEVNDELGNLQRGLS
                     EAVEILEPGGKIAVLSFHSLEDRIAKNFLKQNQSLSLITKKPAVATAQEVKLNPRARS
                     AKLRVAVKQPHVNSLSQ"
     gene            complement(12291..12722)
                     /gene="mraZ"
                     /locus_tag="VFE94_00420"
     CDS             complement(12291..12722)
                     /gene="mraZ"
                     /locus_tag="VFE94_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZX49601.1"
                     /translation="MFLGEYTYSIDEKKRLAIPTKFRQVLGKKAVITRGLDRCLFLYS
                     FKEWQQLADKLSKLPLAQADARGFARLMLAGAMEGDLDKLGRVLVPDYLKEYASLNKK
                     VVIAGVYNRIEIWDEGKWLAYKTRIEKEVGDMAERLKELGI"
     gene            complement(13237..14364)
                     /locus_tag="VFE94_00425"
     CDS             complement(13237..14364)
                     /locus_tag="VFE94_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49602.1"
                     /translation="MLEAKQKIFLIAIASLLVGVTSVQALLVTHIWDPSWNPYRPSPE
                     QVLAQMVQKSKELNTFHLVQNFHATGKTSKGNFDFDFRFASDIDQSSLDALKAHSSFS
                     IDLSSNAHLFTAQPTQTPSLILDHLALEGEVISIDGTSYVRLSSLSLPESFETFFDLF
                     YFGLDPNQLTNQWIKIDQKELLAFSGISPEMAAQELQEQTQLQIELQEILLSPQFYVI
                     QQELPDEEVDGRKMHHYVLEIKVAEMIDAIFAKIFEQEDFQQDQFAAEIAKGAIKGAV
                     ETFIEKIGPITSDMWIDKQTLYLHKATIEKTLDVKKIDQTLEGTSSFEYEMTYSDFNK
                     SVAISAPAQSTSLQELFETLLEPLILQQYYEQSLFNPTLQQ"
     gene            complement(14372..>14704)
                     /locus_tag="VFE94_00430"
     CDS             complement(14372..>14704)
                     /locus_tag="VFE94_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49603.1"
                     /translation="GIYRPEFINRFDGVILFTPLSKQHLMKIAGLMLQKLQSNLREKG
                     IEFVITLPLKERIAELGYNPAFGARNMRRVLQDKVENAVAVAILSNTLRRGDRVTVDP
                     GTFAVNHV"
BASE COUNT         3509 a         3441 c         3698 g         4056 t
ORIGIN      
        1 atcctaaagc aattcaaaaa ctcattgaag cgttgtcccg cttccctacg gttggccccc
       61 gaacagccgg ccgctttgtt ttttatttgc tcaaggcgcc agaccaggaa atcgaagaat
      121 tggcgcaggc cattcgcaac ctaaagaagg aaattaaact gtgcggtttt tgttttaacc
      181 cctaccaggc gacagaagat gaaaacaagt ctggtttatg cggaatttgc cgcgaccagt
      241 cgagagacca tgccatcatt tgcgttgtcg aaaaagaaag cgacctggag gctttggaaa
      301 ggacaaaaaa ttataagggc ctgtatttta ttttgggtga ggcgatgaac atgctcaaaa
      361 aagatggtat aaaggaacta aggcgaaaag aactggagga gaggttggaa tcgcccgaaa
      421 aatttggttt gtcaggcgtg aaattccagg aagtaattat tgccaccaat cctaccaccg
      481 aaggcgaggc taccgcgttg tatgtggaac gtttcttggc gcagatgggt gtaaaaacca
      541 cgaggctggc gagagggttg ccggtgggcg gcgagctgga gtatgcagac gaggagacgc
      601 tgcgttcagc attggaagga agaagatgat accattttac gaaaacatat ttcccgctgc
      661 cctattctgg tttttggtag ccatgctttt tgccgccatc gaaattgaaa tcgaaggcaa
      721 gcacggctgg gcggagaaaa cctccacatg gtttcgtacc aaaggcctag cggcaaggct
      781 gtatggtttg gccatgggtg gaaaacctct taccggatac catttattta tgttttttct
      841 gcctttactg gtggtccact cccattttgt catgggcgtg ccgtggtctg tggaggcaga
      901 actcctggcg cttgccctgt attttgtgtg gatgccaacg tgggatttct tgtggtttgt
      961 gttaaatccg tactacggcg taaaaaagtt taaaaaacaa aaggtatggt ggcatgccag
     1021 gtctcactgg ctgtttaaca ttacgccgtt ggactatttg tttggctggg gtttgtcggt
     1081 ggtgcttgca gttgctgcca ccttagttgc cgacgacttg aggttggtat tctggcattt
     1141 gcattttatg gtttggtttt tcttttttac cgtgcttacc attttactcg tggcaccctt
     1201 atacaaacgc tggcatgcaa aaatgcgcca gcgcgacgac cgcgaggcct cgggcatctt
     1261 ccacgcagag tagacccttg ccaaactgtg ctactttgtg tatgtataga atgcttgaga
     1321 gttacactta gattgcgagg agagaaatga cagagtcgaa tgcactttat aatgacctgc
     1381 tcagcagggc gcggcaagcc ctaccttttg tttctagcca tccgagagag ccagggcgtg
     1441 ctgaagctgc gatccttctt gcggaggaat ccggtcagga gcatgacttg aatattgcga
     1501 agcggcttgt cgacgaacta gctgaacccg aggaacgtct tatggggttg gaggatattg
     1561 ctaaaattac ccagcagagc gaagatcgct tagcggccct gcaggcagat gatgcttacg
     1621 agggaacatg gcaaatcgat aaagaattca cgcctgggaa tataagtttc tcacgaagta
     1681 tccttgtttg gttttcacct tatcctatca cgcaagaggc gatatcgagc gagcgtaacg
     1741 aggcgagggc agaacttctg caatcgcacg tttcgcaact ggtagaccaa ggaaggcttg
     1801 aggatgcgtt gtttgtcgca gaaagccttc gtaccgacgc attgatcgag gatggtgagg
     1861 cgcgggagcg tttctggggc agatgcgttg gggctgtcgc cgttgggtac gccagagcgg
     1921 gaaacttctc gcaggcgaac gattttgccg agctgatagt ttttctagaa gatgagtata
     1981 ttgagttgtt ggtggaaatt gcaagaacct tgcggacaac taaagaggac caccctaatg
     2041 ggtagccctc tttttatttc tatttcgctt ctatcgaggt aatctgtact tcagagtaag
     2101 gctggcggtg gcctcttttt ttcttttcgc gctttttggc cttgaattta aaaatcacca
     2161 cctttttggc ttttgcctgt ttgagaatct tgccttgcac tttggcgccc tgcaccaaag
     2221 gcgcgccaat ctccaggccc ttctgtccct ccaaaagcaa gacttcttca aataccacat
     2281 cgctcccctc ttgcacaggg agtttttcga ttttgagctt tttgccaggg gtaactacgt
     2341 actgtttgcc tccggttttg ataactgcca tttctgccat gttgctttga agcatacctc
     2401 aaattcaaca aaaggtcaat cctcgtcccg gtttggcttt tcgagctgca aggggtcgag
     2461 cccgggggct tgctcggtaa tgcggagaac gtctttgtct attttgccga gttccttgcc
     2521 tgcggcgtta taggtgttta cgcttgtgcc cagagagttc cccagtttgt tgtggtattc
     2581 ttcgtaggtt tttaaatgcc tgcccaaatc ttccacgcgc ttgatgattt ttatggcaga
     2641 ctcttcaatt tgcatggctt ttaacccctg caacacggtt tgcaaatatg ccaaaaagga
     2701 cgtgggcgaa acaataataa cccggtatct gccggctgcc cgctgcacca ggttttcggt
     2761 atcgtctcca acggcgccaa tcgtgtttac cagcaggtcg taataaatgg cctcgtgtgg
     2821 aataaacata aaagcaaaat cggtagtacc ttgttccggc tgaatatact ttgcggtttc
     2881 tacaatgcgg tttttcaaat cctgcacaaa gactttttgc aggcgttctt tctcggcagg
     2941 gtctgtggct tctacaatgc ggttgtaatt ttccaaagaa aacttggagt ccacgggaat
     3001 caccttgtct tttacaaaaa ccgcagcatc cacaatggtg ccgttgggaa aaccgtactg
     3061 catttggtag gatcccgggg gcattacgtt tttgagcagg ctttccaaat agtactctcc
     3121 cagcactccg cgctgctttg gattttttaa gatgtcctga aggcttttga gctgctcggt
     3181 aatgttcatt acctgttttt ggccttctga tacacgcgtt aattcctggg taaccgcctg
     3241 aatgagcttg gcagattccg aatattgctg ctggaacatg gtggcagagg ttcccagctt
     3301 gtcgtctacc gcttggcgga gctcggcgat ctggccttga atcatgaaga tttcctggcc
     3361 tccagcccct tgctgttttt ggcccgagcg ccaaaagaaa aacaccgcga cgactaaacc
     3421 cgcaataata aggatataaa tggtgtcttg catgcctcta gcatagcata ccaggggcta
     3481 ctcggggagg ttgccgtaca ggcggataaa aacttccccg gccacaaacg cgatgtacaa
     3541 aagcagcaaa aacacgcctt cttttgctgt aattctgcgg tcggtgcgca ggaaaatcaa
     3601 aaacaggaga gccgaaacaa caagaaaaac gcgtgcaagg gcaaacggcg agaagtcctc
     3661 gatgcgtata ggctgtatga gagccacaat gccaagcacc atcgtcgaca ttacgattac
     3721 ggcccccatt aaatcgccca aaataatgct gctgtttgcg tggcgggccg cggcaatcgt
     3781 gaagtaaatt tcaggcagcg cacttccaaa accaagcacc aaaattccaa tgaaagcaag
     3841 agggagatca aatgcttctg caaaaaataa gactgcgttt acgactcctt gggcggaagc
     3901 cagcagaatg aggatgccga caagagcctt taggatattt ataaaaaatg cgcgaaatct
     3961 ggcaatgggc ggtattttgg tttgggcggg atctgtgtgg tcgaaaacat gagtgtactc
     4021 cccgcgtttg gagaaaagcc aaatactata aaagaaaaat gtaagcaaaa gtactacagc
     4081 gtctcccctg cccagttggt ggtctaaaat gagcagcagg ggaagaatgg caacagcagc
     4141 ggtaataagg gaggatcgtt gcaccaacgg gccgtctccg cgcaagcccc ggccgaaaaa
     4201 aacggcaaga gcggcaacca gcgtaaggtc taccacgctg tttcccacca cgtctccaaa
     4261 cgagagctcc ggcgcgtcgt tgaaggcggc aataattccc acaaacaagt tgggaaagga
     4321 agccaccacg gccatcacga aaaacgcaat gacaaattcc ttccaccgca aatatcttgc
     4381 aatgtgggta acgctgctca caagccagtt tgacgaccat aagagcaaga atcccgaaaa
     4441 gagaaatagc aaaaagtgaa atagcataaa ggtttatatc tcgccgtttg agtcagcttt
     4501 tttatcgcgc tgaataaata cccacttgcc agcctcaatg agaactatat ttattattcc
     4561 aagagcagcc aaaagagtcc agtcaaaaag gttgagaggg cgagtttcaa aaaggcgttg
     4621 caacaggggg atataaatta ctgcaaacaa aaggataaaa ctaaacaata cgcttgccac
     4681 gagccagagg ttggaaaacg ggttgtattg ccatatgttt tttcgcaaac ttttgcaaga
     4741 gaatacgtac aacagagagt ctatgcccaa gcccacaaat agtaccgtct gaatatgctg
     4801 ggggctatag gtgctgttgc ccaaaagcca gtaaaaaagg ccgataagca aaaaatcggt
     4861 aatcacgcta atcacgaaaa taagcgtttt catctctccg gtaagaagcg aagtatttgg
     4921 cttgggcggg cgccgccgca tgatgtcttt ttccgctttt tcaagggtaa gggctatgct
     4981 gggaaaagta ccctccacaa ggttaatcca gagaatttgc agggcggtaa ccggtaaaaa
     5041 gggcgctccc agcaccaatg tgcttccgat aagaatagtt tccgtgaagg tgccagaaac
     5101 cataaaggta ataattttcc ggatgttatc caaaatgacc cgtccttcct taatggctgc
     5161 gggaataata gaaaagttgt cgcccagcag caccaagtcg gcggtttctt tggcgacatc
     5221 ggtgccagac cccagagcca acccaatatt tgctttcttg agcgcgggag cgtcgtttac
     5281 gccatccccg gtcatggcga ttattgcgcc tttttcctgc caggcttcga taatgcgaag
     5341 tttgtggcta ggttccaccc ttgcaaacac agaaattttc ggcagaagtt tttgcagttc
     5401 tctttcgccc agcgcgtcca aatgcctgcc ttccatgata ttgtcgtgtt gggcgggaag
     5461 cccgagctct gcggcaattg cgcgggctgt cagggcgtgg tcccctgtta cgattacggt
     5521 ttgaatgccg gctccttttg ccgcctcaat cgcttctttt acgccttttc gaatggggtc
     5581 tttcaaagcc acaaacccta taaaagttag atctttgatt tggcttttga aaaagtggga
     5641 aagttgttcc tgcgacttgc cagcaagctg gtcttgcggg atttggcgag tggcgcagct
     5701 caccacgcgc aatccttttt gggtaagctc ggcaagggtc gccagggctt gagctttttc
     5761 gtctttcgag acttgagaga gcgaaagcaa tgtttccggt gctcctgaaa cgtaaaggtt
     5821 tgctccctct ggcgtccgat ggaagcttgc cacgtatctg gtggccgact caaaaggaat
     5881 gcgcaagata acgggaaggt tttcttcgag ttttgttttg gaaaggccgg cttccatgcc
     5941 cgcctgcaaa agggcccggt cggtagggcg cccgcggatt atttgttttt ccagcatttc
     6001 ggctggattt tctacaaatg cttcgtttgc aagcatacct gctaaaaggg tttgttcgcg
     6061 gtcggcgaag cgaagcggca cgatttcttc tacctgcatt ttgccttcgg tgagcgtaag
     6121 ggttttgtcg gttgcaataa cggtggttga gcccagcgtt tctgccgcag gcaagctgcg
     6181 caccagcccc tttgctttga gtatgcgttg catcccaatg gcgaggacga ccgtcattgc
     6241 aatgggcaag ccttccggaa tggcggaaac agctatagct acggcgagtt caaacatgtg
     6301 aaagagctcc tcacccaaaa gaatcccctg caggaaaatg atagccgcca gcaggcttac
     6361 cccgattcca ataagccagg aaaaccgtgc aagcttttcc tggtaaggcg tgcgcgtttc
     6421 ctgaacactg cttaccaaag agctgatttt gccgagttcc gtttgcagcc cgatagctgt
     6481 taccgcggct tttccgtttc cctcctcgac tacgctgccc atatacacca tattgtcgcg
     6541 gtctgccaaa ggcgtttttt cgtcgagcac ggtatttttt ttggaagccg caatccactc
     6601 tcccgtgaga accgcttcgt tcacgcgcag attccagctg ctcaaaagcc tgccgtcggc
     6661 aggcactttg ctgccgggat tcagtacgag aatatcgcct ggcaccacgt cttgctgggg
     6721 aatttctttt tcctgggcgg cacgtacaac ccaagcctta tgcttaagaa tttgcttgag
     6781 ttttgccaac gcattggtgg cgcggttttc ctgcgcatat ccgattaccg tattaatgag
     6841 cacggtgccg aaaataacga tggtgtcggt atattcccgg aaaaacagcg taaccaaacc
     6901 ggcagccacc agaataatga taaatgggtt tttgaactgc ttggaaaaga tgaggatttt
     6961 ggaaggcggt ttttcctcgg gaagtttgtt gtatccaaaa aattcccggc gcctggccac
     7021 cgtttgctcc gacagtcccc tttcgaggtc ggattccaga atgtttgcgg cttccctggc
     7081 ggaaactgca tgccaattgg tacgaaaaat gtcttctaac gacatttgtt tattataaga
     7141 ctaatcctgt caaaacaaaa acccagcgat gctgggtttt tgtcggatgt gtatattttt
     7201 gtaaatccta tcgtgtttct ctggcaatat cgcgaaagag ttggtaggct tcgcgcaccg
     7261 catgcgtcgc gtcgcgcagt gctttttgtg cgtctcgaag ctgcacccgg tcaatgtcat
     7321 tggcaataat agtttctttc agttcttcca aagcgtttgt cgctccctgg attttagcgt
     7381 ttgcggcggc aagaaggctt tgcgattcag tagtactttg gccgcgtgct tctaaagcgt
     7441 cgagcgcttc ttcaattttt gcggcgcgcg cagcagctac gtccaaaatg cggtactcaa
     7501 atacgccaat gtgcgccaaa agcatgagtt tccgcacttt ttgctcggta acttctttcc
     7561 ggtgtttgcc aagttgctct gccaaagctt tcagttcttc tatggtttta gcggcttgga
     7621 tttcaaggcg aaactcctca agcgccgcct ggtcggcctc gatttctgcc aaaagagaat
     7681 ttttgagatc ctcatggatt atggggaaac ggtccacctg ctctttgaga agttgcgtat
     7741 gcgccctgaa agcatctacc acatggagca gcatggtttt gcgcattgca acactcgaag
     7801 gattctggtg gaattgttcc cgcgcctggc ggaactgttc cacttttgcg ttaaagtttt
     7861 cggtgtcggg gtttgcgctt tgcgtgtttt cttgcagctt gatatggatt ttttcttcct
     7921 gcctctcttc cctttttctc gaggccgatt ccaaagcacg ctctctcgca gattctgcct
     7981 ttgcccgcag ttcctctacc cgctctttgt gggtttggac gcgctcttcc tgcgcaaaaa
     8041 ccatttgtgt tcccgtgctt gccaccgata taagtatcaa gctgcccaag atgtattgga
     8101 gaaacctttt catagtaagc ctaaaaatcc tcgagcgcat cccccacttc ctctactttc
     8161 atttcttctt cgaaaaaggc gtcaagttct gtagcagagt caatatcctg ctcctccttg
     8221 gcggcttccg cctcaagcgc gacggcgtca aaatcgttta gatcttccat cgtttgctgc
     8281 tcttcaaagt accaatcgct ttgggtgagc agctgttcga tgccctcggc ttcctcgtta
     8341 atgtcggcaa gtgcgaggcc ctggggagca ggctgcgtat atccccaaaa gagcaggaag
     8401 acgagagcga acgcgcttgc agcgaaagta attttccaag ggctaaaaat gcgctcggta
     8461 agggaggaac gcgtcgcctc attctttgtg acaggaggaa gctgccagag aattgtttgc
     8521 aagaggtctt ttcttggctg aagcttgtgt tgtcgctgca aagcctccaa tgccgccaag
     8581 gtttgtttta cctcgtcttg gtgctcggga aagaaagcta aaatttcagc ggtgcttttc
     8641 ccctggtctt tcagttctag gacggataaa agaagttgtt cggagtcttc gggtgtcatg
     8701 atagattgtt ttcttgaaaa tggctgcgca ggattttcag cgcacggcac tgcacttgcc
     8761 tgatggcgcc ttccgatttt ccggtaacgg ccgccacctc tttgttcgaa agctcttcaa
     8821 tgaatttcag ttcgataatg gtttgctggt cttcatttaa aatatgcagg gcttcccgaa
     8881 tcatgcgagc tgtatctccc ttttcggcaa ggcgcgccgg gttttcggtc ttgctttctt
     8941 tattatggag agttttcgag gtgggatcaa gaggaatgtc ttttcgcttg cgccagtaat
     9001 caatggtgct attccgggca attcggtaga gccatgcgga aaatgatctt ccctcttctt
     9061 ggaattccgg aagcgccgac caggccttga gaaagacatg ctgggtaagg tcttcggcat
     9121 cctcctggct ccgtacgcga aagtacacaa accgaaaaat cggagtgaga tagtattcgt
     9181 agagatgaga gaatgcattc tcgtttccac tttttgcctg ttggaccaat tcctcaaact
     9241 cctcaggatt attcattagc ctggtaggtt aagtttataa cgtatgaaga cgggaattgt
     9301 tacgctttct tacaggggcg gtcccaacgg gagtcgaacc cgtgttacca ggatgagaac
     9361 ctggcgtcct aggccactag acgatgggac caacacaagg tctatatcac agattttcgc
     9421 tttattcaac ctcgtagtcg gggggaatct ggtagtaatc gataatatac ttggcgaggt
     9481 cttggaagat gggaactgcc gaatattccg cggtttttgc ggcggggttg tcgagcttta
     9541 ccaaaatgag aaaacgagga ttgaaagcgg gcgcataccc gataaacgat tggatggttt
     9601 ggtcagaata ccctcctttt gctaccccta aggcggccca ggatatttgg gcggtacccg
     9661 ttttccctgc cacgtagtaa ccggggattt gcgcggtttt cgcgtacccg ttttctacca
     9721 cgctggtgag cattgccgtg agctgcgagg aagtacggga agaaagaatg ggcaaagccc
     9781 cttctttggg cttttcctcc agcacaatat ggggttgtgc gggtacgccg ctgtttgcta
     9841 tggcggaaaa agcgcgcact atctgcaggg gcgtcatttc aatcccctgc ccaaacgccg
     9901 cggtggcaaa gttgatctcg tatccctttt ggaactccgt attgcgggaa aacacttcgc
     9961 ccgcaaggcc tatattggtt gcttggaaaa tgccaaactc ttttagataa tcaaggaagt
    10021 tggcgtgtcc aagcttgcgt tcggcgaata ccacgccggt gttaatggag tattccaaca
    10081 cctcggtcat gcttctttct ccccatgtgc gctggtcgta gttgtacacc ttgctccccc
    10141 ctatttgcac aatgccgtag tcaacatagg tggtgctcgg ggttacggcg ccctcgttta
    10201 aggctccggc catagttatg ggcttaaaaa tggagccagg ctcaaagagt ttttggatcg
    10261 cggagttttg aaacacttca aggtcttcca ctttcgaata ttcattcggg tcgaacgaag
    10321 ggatattggc gagcgccaag agctttccgt cggcagggtc cataacaata atactggctt
    10381 tttccacgtt gagctcctgt cggactttct gtaaaagttc ttctgccact gcctgaatgt
    10441 tgtagtcaag cgtaaggaca atatcggaac catcttccac ggtttctctg ttgctcaaga
    10501 aataggttgc cgggttccaa gagcttttga gcaggccttc tttccccccg aggacgtcgt
    10561 tgtagtagcc ttcgactccg tactgccctt tcccttcggt atttacaaaa cctgccacgt
    10621 gcgaggccaa agaaccaagg gggtaagacc ttacattttc ttctcccaaa taaatgcccc
    10681 gaaggttgag ttctgccatg cgggttttct cttcgtcggt gatgcgtttt ttaatgggtt
    10741 catacaggct ttctttgcgc tggagcttgg gcaaaatctc ttcgctaggt atcgaaagta
    10801 tatctgcgag tttttgcgcc tcgtcctgcg catcctgcac ttcaggagga gacgcaaaca
    10861 cataaggcag tttctggttc gtagccagta agtgaaggtt gcctaagcga tcctggataa
    10921 aaatgtctcc ccgcacgcct cgtccataag agctcaaatt ttgctggccc tgggcgagcg
    10981 ctttataaaa cccgtattca aaaatttgca ggtaagccaa acgcgaaaga agccctatgc
    11041 ccatacaaac aatcacaagg agcaaaagag tacgccgcca cccttcttga gtatgggaat
    11101 gatttcgcat ggggtatgga aaaaggccta tggctggttt gcagctacgc ctcccgtggg
    11161 aatcttgaga tatgaaatgt tggtgatttt ttcaaattgc atattctgcg caagttcttc
    11221 caagtcgttg agcgaaaagg tgtggcgagc gcgaatttcc agttgggtaa cgtcctgctt
    11281 gagttgggtg gcaagttgct cttggtctgc aatatggtag gcaagcgcag tgagcgcgtt
    11341 gagttgaaaa acgtacatcc ccacaagaag gccggctatg accaggggaa gaaaccagaa
    11401 tagtttactg tgataaagag ttgacatgag gttgtttaac ggctacgcgc agttttgccg
    11461 atctcgcccg agggttcagc ttgacttcct gggctgtggc tactgctggt ttttttgtaa
    11521 taaggcttag tgattggttt tgtttcaaga agttctttgc gatgcggtct tccagggaat
    11581 gaaaagaaag tactgcgatt ttgcctccgg gctccaggat ttcgactgct tcagaaaggc
    11641 ctctttgcag attgccgagc tcgtcgttta cctcaatgcg caatgcctgg aatgttttgg
    11701 tagctgcatg gatttttttg tgccggtagc ttttcggcgt ggcccgctcg ataacctcca
    11761 ccagttgttt cgtcgtgcga atcggttttt gtgcccgaac ttcaattatt tctgctgcga
    11821 tttgttttgc atattgttct tcgccgtatt ctttgaaggt gtgttctaac tgggttctcg
    11881 agtagtaatt gaggattttt tctgccgtgt ctgggttgtt ctggttatag cgcatgtcga
    11941 gaggttcatt tcgcataaaa gaaaatcctc tttgcgactt ttctatctgg tttaaagaaa
    12001 atccgagatc gaacagaatt ccgtgcaccg ggtgaaactt ctcttgccgg gcaatttctg
    12061 caatatcggc atagtttcct tctcgcgcca gtaatctatt ttggggtttg agaagcgaga
    12121 tagcgttcgg atcccaatct atggcaagaa cctttccttt cggtgctgcg agttccagta
    12181 tcgcgaaggt atgcccgcct gctcctgccg tgcaatcaat aaaattttgg ttgggttgcg
    12241 gattgagcag ctccaagact ttttccagaa gaaccggagt atgcatgggt tcaaatgcca
    12301 agttctttta atcgttctgc catatccccc acttcttttt caatgcgggt tttgtaagct
    12361 aaccatttgc cttcgtccca aatttcaatg cggttataca cgccagctat caccaccttt
    12421 ttgttcaaag aggcgtattc tttcaggtag tcgggaacga gtactctccc aagcttgtcc
    12481 aaatccccct ccattgctcc tgccagcata agtcttgcaa aaccacgcgc gtctgcctgg
    12541 gctagcggca gtttgcttaa cttatcggcc aattgctgcc actctttaaa agagtacaaa
    12601 aaaagacacc ggtctagccc acgcgtaatt actgcttttt ttccaagtac ttgccggaat
    12661 ttcgtgggga ttgcaaggcg ttttttctca tcgatggaat aggtgtattc gcctaaaaac
    12721 atgaaagcta ttggtgagtt ttccacagaa atgccgagtt ttccccactt ttatccacag
    12781 ttcaccacca atgctccact atataccaca caaggacccc agtcaaaccc cacttatcca
    12841 cagcaataac aataaaaaac agccccaaga aatccggggc cgcttatttt agatgcagcc
    12901 tagtattggc agcatttcgt aggtattggg tctggctggg ttatagggta taaccgccaa
    12961 gcttcctgat aaactcgatc gaactctcct ttcgtgattt ctccatctag gagcttccgg
    13021 cttgcttctt taatcattct gtttctttcc agagtgctca tttccagctc ctttaagaca
    13081 gtctgcttct atgctaattc tgtaaacaaa aaagtcaagg agaaagtatc acatcctaat
    13141 ttccacagtc cccttgttca agcgatcgcg tgaacaaggg acaaatcctt tgcatacctt
    13201 aaaagcagcc tttcgacttg cgtcagggcc gtttttttat tgctgtagag tgggattaaa
    13261 aagagattgt tcgtagtact gttgaaggat gagaggttcg aggagtgttt caaaaagttc
    13321 ctgaagagaa gtgctttggg cgggagcaga aatcgcaact gacttgttaa agtcagaata
    13381 cgtcatttca tactcaaagg agctagtgcc ctcaagggtt tggtcgattt ttttcacatc
    13441 taatgttttt tcgatggttg ccttatggag atagagcgtt tgtttgtcga tccacatatc
    13501 agaggtaatg ggccctattt tttcgatgaa tgtttccaca gcccctttga tagcaccttt
    13561 tgcaatttcc gcagcgaatt ggtcttgttg aaaatcttct tgctcaaaga ttttggcgaa
    13621 tatggcgtct atcatttccg ccaccttaat ttccaaaaca taatgatgca tttttctgcc
    13681 gtctacttct tcgtcgggaa gttcttgctg gattacatag aactggggtg acaagagtat
    13741 ttcctgcaat tctatttgta gttgagtttg ttcttggagc tcttgtgcgg ccatttctgg
    13801 ggaaattccg gaaaacgcca gcagttcttt ttggtcgatt ttgatccatt ggttggtcag
    13861 ttggttcgga tcaagaccaa aatagaaaag atcaaagaat gtttcaaaag attccggcaa
    13921 ggataaagag gaaagacgaa catatgaggt accatctatg cttatcacct ctccttctaa
    13981 ggcaagatga tccaagataa gagagggcgt ttgtgttggt tgcgcagtaa ataagtgagc
    14041 gttactgcta aggtcaatgc taaaagaaga atgagctttg agtgcgtcga ggctagattg
    14101 gtcaatatca ctggcaaagc gaaagtcaaa atcaaagttt cctttacttg tctttcctgt
    14161 cgcgtggaag ttctgtacaa gatgaaaggt gttcagctct ttcgattttt gcaccatttg
    14221 cgcgagtact tgctcgggtg aggggcggta cgggttccac gaagggtccc atatgtgggt
    14281 tacaagtaaa gcctgaaccg atgtgactcc aacaagcaag ctggcgatgg caatgaggaa
    14341 aattttttgt ttggcctcga gcataactac attatacgtg gtttacggca aatgttccag
    14401 ggtctaccgt aacgcgatcg ccccggcgaa gggtgttcga caaaatggca accgcaacgg
    14461 cgttttccac cttgtcttgc agcacgcggc gcatgttgcg ggcgccaaac gcggggttgt
    14521 atccaagctc tgctattctt tctttgaggg gtagggttat aacaaactca ataccctttt
    14581 cccgcaggtt gctttgcagt ttttgcagca tgagtcccgc gattttcata agatgttgtt
    14641 tgctcaaagg cgtgaacaga ataacgccgt cgaaccggtt aatgaactcg gggcggtaaa
    14701 tccc
//
LOCUS       DASRUP010000008         7618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1406883, whole genome shotgun sequence.
ACCESSION   DASRUP010000008 DASRUP010000000
VERSION     DASRUP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406883"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VFE94_00435"
     CDS             <1..1022
                     /locus_tag="VFE94_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49604.1"
                     /translation="GGASGRFHCGRFGGGFISSDYLSGGGGGGGGSSYVDSAFEVSGS
                     TTHTQGCATCRGAGAVAISYVGLPLPGSTAQDEEFFNIDLTAPTVTNFTASDGTTTVN
                     ASNDPLVAMSSPVTLEWGAADAPGGAGLGAQELWQAAYIAGLCDENDISRCSWVQRFT
                     NATSPATDTPPLGKWWYSVRAYDQSLPSGNCITDQGAHCGGTPGPDRLDPRTAIGPIL
                     IEMPTIGGLVPCGRQTDSIDTPWNETENCEFRHFFLLARILVDFTLWRAVPLIIAVLL
                     LATGAIFYFSFGGPGAMARVRQIWRAVLLGSLILIFSWLFLNFLLGIIGFDINVFGRW
                     YDVQL"
     gene            1206..1754
                     /locus_tag="VFE94_00440"
     CDS             1206..1754
                     /locus_tag="VFE94_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49605.1"
                     /translation="MKISRIGQFLVLLVVLGTLVACAEASTADQENGDVSLAPGPIPA
                     TIDGCRRAVAALANHGTSTYDINLPVVDSQYRSFLAFVQEKRGIMPQISGWGIENSYV
                     RPDGVAVVQYKVLGLYNTPDLWVQEQVAVVTFECVTRDITNPNFFPEVYALNGVDFRD
                     FMESHNFPKPYYATLLGNMSWQ"
     gene            1834..5568
                     /locus_tag="VFE94_00445"
     CDS             1834..5568
                     /locus_tag="VFE94_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HZX49606.1"
                     /translation="MHLAHKKILIVFLVAVVLGGFLLSPAPAYAQPQCFILGVVCFFV
                     NWIGETVTDTIVTGIAKIFLVVTTTIPAAIFFLVAGITNWIIAFLIQIPVSPGNSASP
                     MFVQQGWEFTRQFVNIFFLVILVFIGLATILRFREYELQRTLPRLIVVALLVNFSGVL
                     VGFVTDVGNIGTITFTQSIGNVFGDTKSGQHFAGVQETAMAKIDALRSAPNGDSLQVW
                     GTVFALAFALWIFYLFASVMFGILAITFFVRQAILWLLVILAPLAFLAAVLPATRGWW
                     NQWLKALMQWAFIGVPLAFFMWLAQNILVNPVFPAGTSPQLQGNTNVFQDIIAAMITP
                     FTAMIILGLGVLLSYGLAGRMSGAVSGGITKQVRRLPRRTWDSRTVQRAQGKLAGAIE
                     RSFGDPNRRMSREMKERADQSTGAGKLGWGAAAAATNVTSRIPMFGFMRRKLIETEHA
                     KRRLPLPQGFRDWDPRLQEDWIKTHGSDEDAIQGIAQIAEAGNFRYMSEDMQKRILEI
                     ADRVDKMGDRTFQSELYAIRRANPENHEFWHDLSEEMKEEGVNPLDQERLQRIVEASL
                     KATPRKEDFTKDDFAPALSLSPNVDPRHFAEAVNDLRKKINTEKDRLENALNEEGETG
                     KEGLTIDLALKYRLITNEEVQADRAAALATADRELKNKFAQEGKNFSEEIEKHTESLA
                     ATSVFVGDMRSRFIQNLGNTTDTGVRFGLAANPQHLRQVLEHHGRSALNRILNGSAGI
                     NRRTGTPEGVDDFVLANPQTHNFFDTIIGREFPYAGRDNMRDAWGNPTTSRQRHGDRI
                     RILRLAQEDKNGLVKSYYDILQTQRSAQGTLQRAQSQGAPAADIQNLEDAINQAQADI
                     QSIDDFYQLNTDLNQGLLNKYGIKQMSDPEAEANRRRLTEVRRLLNTTGTASGRRRGG
                     GNNGGNQQGGGRRGRRNPPSGGGGPRRIVYNPTNPNGGGGNGGASPTGGGTPTGGGGP
                     NPNTGGGSSAANPSPAASSNPPAGEGRGYQNAAEQREDGQAGSTGTNATSAPRWLTEG
                     IEKVDQGSIPLTIASRLEKYLQDNGVSQQEINAMDPKTAWQRAKEIRGGSPGPRGPAP
                     QGAAQPLLDLGGGVQVLDMFEKAGRRVFVVKMPDGSVRPFYRSTGQGAPGQKLEGDIN
                     PFGGWLEKGQQVFGAQRMPRDWFIKGGPGIGGDLAFVEEGDDKRLHPADPQLRAIAEQ
                     IHKQEALWGEQTVDEIYTPERANERLRKYGAIMKDDYIGG"
     gene            complement(5675..6334)
                     /locus_tag="VFE94_00450"
     CDS             complement(5675..6334)
                     /locus_tag="VFE94_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49607.1"
                     /translation="MWEFNVQNSYSLGFWGFLAVGMLVGLVIWAFKRGGAQQPPAAPP
                     TPPAPLAPAPPTVGQQNAFQSLAQAQARILQNSTYLLESAELSVQTALTNKRADERQK
                     YADDIEQGRSGTNGGAQTGGGGTQRGTSYTLTVNGAPVGQGQTRVDIAGGYVNLTPAP
                     GTQGYAPNTRVTLTVVPDALGATASFHGAGQRGPLEGVVTLRNNRNVTVTIVPPTNPS
                     P"
     gene            6418..7020
                     /locus_tag="VFE94_00455"
     CDS             6418..7020
                     /locus_tag="VFE94_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49608.1"
                     /translation="MSPVQKPYTEEQFWRMYEKLPPDVKDALWGTETGDAIYDVCQKN
                     GAQEQHADVLQLCTNVFLGLMLPQELEKALVEKAGLDQTTATNIAAQLNGLIFYPVKA
                     GLEQLHQQVGKTDKQGSPVGIPTPRHAGRIPAASPYTVAPEEQTAEPQPEPQQETPAS
                     SPVKRRSVPRRQAGAPPPSAPPGPDVGPEPSEDPYRETAE"
     gene            7023..7439
                     /locus_tag="VFE94_00460"
     CDS             7023..7439
                     /locus_tag="VFE94_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49609.1"
                     /translation="MAQYQTPQFIEREPKVIGPLTFKRAAYLGFPLAIIFILWFLIAE
                     NYLMIFVLISVVLEGAGVVLAFFKVEGKSVPMLLTDAFFFVTRPKTFVWQRNNAKLDF
                     HEREYVNPLGSQGLRKDELSRQSRVADLATKVQTRK"
BASE COUNT         1950 a         1896 c         2002 g         1770 t
ORIGIN      
        1 gcgggggtgc gagcggaaga tttcattgcg gccggttcgg tggaggtttt attagtagtg
       61 actacctgtc tggcggaggt ggaggtggcg ggggttcgag ctatgtggat tctgcttttg
      121 aagtgtcggg ttctactacg catacccaag gctgcgcgac gtgccggggc gcgggagccg
      181 ttgccattag ctatgttggc cttcctttgc cagggtcaac cgcccaggat gaagagtttt
      241 ttaacatcga ccttaccgcc ccaaccgtaa caaactttac cgcttcagat gggacaacaa
      301 cggtaaatgc aagcaacgat cctttggtgg caatgtccag ccctgtgaca ttggagtggg
      361 gcgcggcaga cgcgccggga ggagcggggc ttggcgccca agaactttgg caagcggcct
      421 acattgccgg cctttgcgac gaaaacgaca taagcagatg ctcgtgggta cagcgattta
      481 ccaacgctac ttcgcctgcc accgacactc ctcctttggg caaatggtgg tatagcgttc
      541 gagcttacga ccagtcattg ccttccggca actgcattac agaccagggg gcgcactgcg
      601 gcggaacgcc ggggccagac aggctcgacc ctagaaccgc tattggcccc attcttattg
      661 aaatgcccac tattggcgga ctggtacctt gcggccgcca gaccgactct atcgacactc
      721 cgtggaacga aaccgagaac tgcgaattcc gccatttctt tttgctggcg cgcattctgg
      781 ttgactttac gttgtggcgc gccgtgccgt tgattatcgc ggtgttactt ttggccaccg
      841 gcgccatatt ttacttttcg tttggcgggc caggcgctat ggctcgcgta cgccaaattt
      901 ggcgcgccgt gcttttgggc agccttatcc ttattttctc gtggctgttt ttgaacttcc
      961 tactcggcat tatcggtttt gatattaacg tttttggcag gtggtacgac gtgcagctct
     1021 agaatgtcgc tcgagagttt tccacacccc tttgctttat tttctaaaga aaaatctcct
     1081 ttttgcttta catgcttgac atttttcccc gaatgtgcgt aagataaata tagaagtggg
     1141 gggtttctct ttacaaaacc tcttacgcct aaaattagct caactacgtc gtcaaggagg
     1201 acgaaatgaa gatatcgcgg ataggccagt ttttggtcct tctggtggta ctgggcactc
     1261 tcgtcgcttg cgccgaggca agcacagccg accaagagaa cggagatgtt tcactagcgc
     1321 caggccccat acccgcaaca atagacggct gcagaagggc ggttgccgct cttgcaaacc
     1381 acggaacatc aacctacgac ataaacttgc ctgtcgtaga tagccaatac cggtctttcc
     1441 tcgctttcgt gcaggaaaaa cgggggataa tgcctcagat tagtggctgg ggaatcgaga
     1501 attcgtatgt gcggccggac ggagttgccg tagtccagta caaggtgctg ggactataca
     1561 acactccaga cctatgggtg caagaacaag tcgcggttgt cacattcgag tgtgtcacgc
     1621 gggatatcac caaccctaac tttttcccgg aggtgtatgc cctcaacgga gtagacttta
     1681 gggatttcat ggaaagtcac aactttccca aaccctatta cgcgaccctg ctgggaaata
     1741 tgtcctggca gtaaaaggag aatcaacgct ccttttttta ttttgtccac acttttgctt
     1801 gtgtttcttt ttcgctctgc atataataag ctaatgcatt tggcgcacaa aaaaatccta
     1861 atcgtttttc ttgtcgctgt tgttttggga ggttttcttt tgtcccctgc gccagcctat
     1921 gcgcagccgc agtgctttat tttgggtgtt gtctgcttct tcgttaactg gatcggggaa
     1981 acagtaaccg acacgattgt aacaggcatt gcaaaaatat ttctcgttgt tactaccact
     2041 atacctgcgg caattttctt tttggttgca ggcattacca actggattat tgcgtttctc
     2101 atacaaattc ccgtatcgcc gggtaattcc gcctccccta tgtttgtcca gcaaggatgg
     2161 gaattcacgc gccagtttgt aaacatattt ttcctggtca ttttggtatt tattgggctg
     2221 gctactattt tgcgcttccg ggagtacgaa ttgcaacgca ctctgcctcg gcttatagtt
     2281 gttgctttat tggtaaactt tagcggcgtg cttgtgggct ttgtaacaga tgtgggcaac
     2341 attggcacca taacatttac ccagtctata ggaaatgtgt ttggcgacac gaaaagcggc
     2401 cagcactttg ccggtgtgca agaaaccgca atggccaaaa ttgacgcact tcgtagcgct
     2461 cccaatggtg actcgttgca ggtatggggc acggtattcg cacttgcttt tgcattgtgg
     2521 attttttacc tttttgccag tgttatgttt ggcatacttg caattacctt ttttgtgcga
     2581 caggccatac tttggctatt ggtaattcta gctcccctgg cgtttcttgc cgccgttctg
     2641 cccgcaaccc gcggctggtg gaaccagtgg cttaaagccc ttatgcagtg ggcgtttatt
     2701 ggcgtgcccc tggcgttttt catgtggctt gcgcaaaata ttctggtaaa tcccgtgttt
     2761 cctgctggaa caagcccgca gctccagggg aatacaaatg tttttcagga cataatcgca
     2821 gcaatgataa cgcccttcac cgcaatgatt attttggggc ttggagttct cttaagctac
     2881 ggcctggccg ggcgaatgag cggagcggta tcgggcggta ttacaaagca ggtgcgcagg
     2941 ctgccaagaa gaacatggga ttcccgcaca gtccagcgag cgcaaggcaa gcttgccggc
     3001 gccatagaac gctcgtttgg agacccgaac agaagaatgt cgcgcgaaat gaaagaacgg
     3061 gctgaccaga gtacaggcgc cggcaagctc ggctggggag ccgcagctgc cgcaacaaac
     3121 gttacctcgc gcatacccat gtttggattt atgcggcgca aactcattga aaccgaacac
     3181 gccaaacgcc ggcttcctct tccgcaaggc tttcgtgatt gggaccctcg ccttcaggaa
     3241 gactggataa aaactcatgg cagcgacgaa gacgcaattc agggcattgc tcaaattgcc
     3301 gaagccggca acttccgcta catgtctgaa gacatgcaaa aacgcatcct cgaaattgcg
     3361 gacagagttg acaagatggg cgacagaacg ttccaaagcg agctctatgc cattcgccgg
     3421 gcaaatccag aaaatcacga gttctggcac gaccttagcg aggaaatgaa ggaagaagga
     3481 gttaaccctc tcgaccaaga aagactacaa aggattgtcg aagcaagcct gaaagcaacc
     3541 ccgcgcaaag aagactttac aaaagacgac tttgcgcccg cacttagcct tagcccaaat
     3601 gtagaccccc gacacttcgc agaagctgtt aacgaccttc gaaagaagat caacacagag
     3661 aaagacagac tggaaaacgc gttaaatgaa gagggcgaga ccggcaaaga gggccttacc
     3721 attgatttgg cgctcaagta cagacttatt accaatgaag aagtacaagc ggaccgcgca
     3781 gctgctcttg ctactgccga cagagagctt aaaaacaagt ttgcacaaga aggtaagaac
     3841 tttagcgaag aaatagaaaa acacacagaa agccttgccg ccacttcagt gtttgtgggc
     3901 gatatgcgct cgcggtttat tcaaaacctc gggaatacta ctgatacggg cgtgcgcttt
     3961 ggcctagctg ccaaccccca gcacctgcgc caagtgttag agcaccatgg ccgctcggcc
     4021 cttaaccgta ttctcaacgg atcggcaggc attaaccgaa ggacgggaac gccagaaggg
     4081 gttgatgact ttgtgcttgc aaaccctcaa acccacaact tcttcgacac cataataggg
     4141 cgagaattcc cctacgcagg aagagataac atgcgtgatg catggggcaa cccaactacc
     4201 agccgacaac gacatggaga tcgaataaga attctgcggc ttgcacaaga agataagaac
     4261 ggcttggtaa agtcgtacta tgacattctc caaacccaga gaagcgcgca aggaactctt
     4321 caaagagccc aatcgcaagg cgccccagct gcagacatac aaaacctcga agacgcaatc
     4381 aaccaagcac aagcagacat acagtcgatt gatgacttct accaactaaa caccgacctt
     4441 aaccaaggac tattaaacaa gtacggtata aaacaaatgt ctgatccgga ggccgaggcc
     4501 aacagaagaa ggcttacaga agtacgccga ctactaaata ctactggaac ggcttctggg
     4561 cgcagaaggg gaggtggaaa caatggagga aaccagcaag gaggcggaag acgtgggcga
     4621 agaaacccgc cgagtggcgg aggtggacca agacgcatcg tctacaatcc aacgaacccg
     4681 aacggaggcg gaggtaatgg cggcgcctca cccacaggag gtggaacgcc cactggggga
     4741 ggagggccaa accctaatac gggtggtggg agctctgctg caaacccatc acccgcagcg
     4801 agcagcaacc cgccggcggg agagggtaga gggtatcaaa atgcagcaga gcagagagag
     4861 gacggacaag ctggctcaac agggacaaat gcaacaagcg ctcctcgttg gttaacagag
     4921 ggtatcgaaa aagtagacca aggaagtatt ccgttaacaa ttgcgtcaag gcttgagaag
     4981 tatttgcaag acaacggcgt ttcccagcaa gaaataaatg caatggatcc gaaaactgcc
     5041 tggcaacgag caaaagaaat ccggggaggt tcgcccggtc cgcgtgggcc agcgccgcaa
     5101 ggagcggctc aacccctgct tgatcttggg ggcggggtac aggtacttga tatgtttgag
     5161 aaggctggca gacgtgtatt tgttgtaaag atgccagatg ggtctgttcg ccctttttat
     5221 agatcgactg ggcagggagc gcctggtcaa aaactggaag gtgatattaa tccttttgga
     5281 ggttggcttg aaaaaggaca gcaagttttc ggagcacaaa gaatgccaag agattggttt
     5341 attaaagggg gtcctggtat tggtggagac ttggcatttg tagaagaagg tgatgacaag
     5401 agattacatc cggcagatcc acagctccga gcgattgcgg aacagataca caaacaagaa
     5461 gctttgtggg gggaacaaac cgtagatgaa atatataccc ccgaaagggc taatgaacgc
     5521 ttaagaaaat atggggcaat tatgaaagat gattatattg gcggttagaa aattggatat
     5581 gaaaattagc agaattgtaa agctcttaaa aaataaaacg agggagaagc gaatcggcac
     5641 atagtgcttt tcgctcctcc cttactggag tctgttacgg cgaaggattg gtagggggta
     5701 cgattgttac ggtaacattt ctgttgtttc tcaacgtaac aactccttca agaggcccac
     5761 gctggcctgc tccgtggaag cttgcggtgg ccccaagagc gtccggaact actgttaagg
     5821 ttacgcgcgt gtttggcgca tagccttgag tccccggggc gggcgtaagg ttaacgtagc
     5881 ctcccgcgat atccactcgc gtttgccctt ggcccaccgg agccccgtta accgtaagcg
     5941 tatagctggt tccgcgctgg gtccctccgc cgccagtttg ggcgccaccg tttgtaccac
     6001 tccgcccttg ctcaatgtcg tcggcgtact tctggcgttc atccgcgcgc ttattcgtaa
     6061 gcgcagtttg aacgcttagc tcggccgatt caaggagata cgtagaattt tgtagtattc
     6121 tggcctgtgc ttgggcaaga ctttgaaacg cattttgctg cccgacggtc ggaggcgcag
     6181 gcgctagtgg ggcaggtggc gtgggcggag cggcaggagg ttgttgagct ccgcctcgct
     6241 tgaatgccca aatcaccaaa cccaccagca tccccacggc caagaatccc cagaaaccca
     6301 aactgtagct gttttgtacg ttaaactccc acattccttc tttactcctt atgtactgtc
     6361 ttacgtgcta aaattgtgta tatattatag atcataagtt taaaaatgtc aaggtctatg
     6421 tctcctgttc aaaaacccta taccgaagaa caattctggc gcatgtatga aaagttgccg
     6481 cccgacgtga aagacgccct atggggcacg gaaaccggcg acgccattta cgacgtatgc
     6541 caaaaaaacg gcgcccaaga gcagcacgcc gacgtgttgc agctttgcac caatgtattc
     6601 cttgggttga tgctgcccca agaattagaa aaagcgcttg tggaaaaagc cgggctcgac
     6661 cagactactg ccaccaatat tgccgcacag cttaacggac tcatttttta tccggtaaaa
     6721 gcggggctgg aacagcttca ccagcaagtg ggaaaaacag acaagcaggg ctcgccagtg
     6781 ggaattccca cgccaagaca cgctggccgg attcccgctg cctccccgta cacggttgcg
     6841 ccagaggagc aaacggcaga accgcaacca gaaccccagc aagaaactcc tgcttcgtcc
     6901 ccggtgaaaa ggcgatctgt tccaagaaga caagcggggg ctccgccccc gtcagcgccg
     6961 ccggggccgg atgtcgggcc tgaaccctcg gaagacccgt accgtgaaac ggcagaataa
     7021 ccatggcgca atatcaaacc ccccaattta ttgagcgaga accaaaagtt attggcccct
     7081 taacatttaa aagggcggcg taccttggtt ttcccctcgc catcattttt attctctggt
     7141 ttcttatcgc agaaaattat ttgatgattt ttgttcttat ctcggttgtt ttggaagggg
     7201 caggagttgt acttgctttt tttaaggtag agggcaaatc tgtgcccatg cttttgacgg
     7261 atgccttctt tttcgttacc cggccaaaaa cgtttgtgtg gcagcgcaac aacgcaaagc
     7321 ttgatttcca tgaaagagaa tacgtaaatc cgctgggcag ccagggcctg cgcaaagacg
     7381 aactctcccg ccaaagcaga gtggcagatt tggccacaaa ggtacaaaca aggaaataaa
     7441 tctggtataa tcgatatatg gcaggttcta cacaggaatt tttagagttt caggaaatac
     7501 gggagggcac tattgttatg aacgaccgct ccatacgcgg agtgcttttg gtatcttcaa
     7561 ccaactttgc cctaaagtcc gcagaagaac aagacgccat tattttccag tttcaaag
//
LOCUS       DASRUP010000009         5215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1245255, whole genome shotgun sequence.
ACCESSION   DASRUP010000009 DASRUP010000000
VERSION     DASRUP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245255"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFE94_00465"
     CDS             <1..381
                     /locus_tag="VFE94_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49610.1"
                     /translation="YDRNIMSRRIAKKEGGLSRVYFEVVSKLSVKIRTTEQYWNIITK
                     IKHPVMRGKERVVQKALQEPNIIRRSRVDKNVYLFYRKYQKHHLVVVVRHLNKREGFI
                     ITTYITDIIKEGIQVWPKQSKTIK"
     gene            378..593
                     /locus_tag="VFE94_00470"
     CDS             378..593
                     /locus_tag="VFE94_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HZX49611.1"
                     /translation="METIRVYYDSVGNTLNVWFADPRKEFMSEESGDEVILNKDRNGK
                     VIGFEKLNYLRKKIDSLKKLPVEVAIS"
     gene            596..1706
                     /gene="prfB"
                     /locus_tag="VFE94_00475"
     CDS             join(596..649,651..1706)
                     /gene="prfB"
                     /locus_tag="VFE94_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152965538.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZX49612.1"
                     /translation="MADMQTQLEELEGKLRYLRTVFDLPEKKDKIVSLQKESEKPEFW
                     QDGETSSRLSQELAELREIVQEFERLQKDFEDLKELAKLSQRDEDAAAELPAILESLQ
                     KRIAKQETQTFLSGKHDRANAIFTITAGAGGQDAADWATMLQRMYERYCERKGWKVSI
                     LHQSFGDPGPEGRIGTKNVSLEVRGAYAYGLLKRETGVHRLVRISPFSAKSLRHTSFA
                     AVEVLPEIDVQKERGIELKPDDLHIDFFRASGPGGQNVNKRETAVRITHIPTGIAVAS
                     QAERSQQQNKEKALQVLASRLYQMAEQERAKEVAKLQGQQAAIEWGSQIRSYVLHPYR
                     MVKDLRTGLEVSNTEAVLSGDLDEFIEAQIRIGND"
     gene            1699..2382
                     /gene="ftsE"
                     /locus_tag="VFE94_00480"
     CDS             1699..2382
                     /gene="ftsE"
                     /locus_tag="VFE94_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZX49613.1"
                     /translation="MISYREVTKIYPDKSIALRDISFDIAKGEFVSLVGKSGAGKTTL
                     LRLLLAEERPTQGQIVFDNLDVHQIKRSKLPELRRSIGMIFQDYRLLDSKTAAENIAY
                     VMEVMGLDEATIERDVKEVLEIVGLTERADHYPTQLSGGEKQRVAIARALIHRPRVIV
                     ADEPTGNLDPYHTREVINLLVKINELGTTVMLATHNREVVNRLAKRVLTFSEGRLVRD
                     EENGKFIVL"
     gene            2386..3291
                     /locus_tag="VFE94_00485"
     CDS             2386..3291
                     /locus_tag="VFE94_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZX49614.1"
                     /translation="MFLGVKRIVKSGTEKFLRDRSSTGAALVVMVIVLFVVSTAYILQ
                     GVASFLVASLEESVDVSAYFYDAVSEEQIFQVQEQLKTLAEVKEVEYVSKDEALSRFV
                     ELHRDDEEILEPLQALGRNPLRASLNIKAHEPGQYGAIAEFLSHTSFSEMLADVDFSE
                     RAPLIERLGAITAGVRATILAVIIALGAIGALVAFNTIRLAIYSSREEIEVMRLVGAS
                     NWFIRGPFLIQGVLVGVAASCITMLFALGVAFGISGQLEAFSGFNVTQFLLGNFFMLL
                     LLQLIVGIGLGIISSIVAIRRYLQV"
     gene            3401..4336
                     /gene="fabD"
                     /locus_tag="VFE94_00490"
     CDS             3401..4336
                     /gene="fabD"
                     /locus_tag="VFE94_00490"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZX49615.1"
                     /translation="MFPGQGSQSVGMGLEIYKASKAAREVFEEADDSLGFPISRLIFN
                     GPEEELQDTVNSQPAIMVVSIACWKAWEEFLGTKKKKKQQGKPSPVSVAGHSLGEYTS
                     LVVVGALPFAEALWLVRERGRLMRQASIEHPGAMAAILGLDEFALEHICAETGVEMAN
                     VNTDDQIVISGDRLAVARAMDLASIRGAKKTISLHVSGAFHSSLMSQAREGLAAAVEA
                     VNFYDPHVPVIGNYTGTPLTSADQLRDELLEGLCHCVQWRNSVRYMASAGVTHFVEFG
                     PSKVLSSLIKRIDGDVEAVTLSEPASMEKFAKELR"
     gene            4401..4476
                     /locus_tag="VFE94_00495"
     tRNA            4401..4476
                     /locus_tag="VFE94_00495"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4434..4436,aa:Gln,seq:ctg)
     gene            4618..4875
                     /locus_tag="VFE94_00500"
     CDS             4618..4875
                     /locus_tag="VFE94_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZX49616.1"
                     /translation="MYYVYLLENQDDKSWYTGFTSDLRQRVKEHKEGKGGRTTALKRH
                     WKLIYYEAYLNKNDALGREKFLKSGSGRRYLKKQLKHYLSI"
     gene            4919..>5215
                     /locus_tag="VFE94_00505"
     CDS             4919..>5215
                     /locus_tag="VFE94_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HZX49617.1"
                     /translation="MPHDNGEKAMQFAIKYERRRTGETPDDVSREGRGYDLESKDRLI
                     EVKGTDHVNGGLVISLYKKLLTKLGKDIVKYHIYLIYDMRREPKLKILTPEFIFA"
BASE COUNT         1451 a         1114 c         1404 g         1246 t
ORIGIN      
        1 tatgatagaa atattatgag ccgtaggatt gcgaagaaag aaggaggttt gtcgagagta
       61 tattttgagg tagtttcgaa acttagtgta aagataagga cgacagagca gtactggaac
      121 attatcacga aaatcaagca tccagttatg cgaggaaaag agcgtgtggt tcagaaggca
      181 ctgcaagagc cgaacattat ccggaggagc agggttgata aaaatgtcta tttattctat
      241 agaaaatatc agaagcatca ccttgtcgtg gtcgtgcggc atctaaacaa gagagaaggt
      301 tttattatca caacatatat aactgatatc attaaagaag gaatacaagt atggccaaaa
      361 caatcaaaaa caatcaaata gagacgattc gggtatatta tgacagcgta gggaatacgt
      421 tgaatgtttg gtttgctgat cctcgcaagg agtttatgtc agaagaaagc ggagatgagg
      481 taattttgaa taaagacagg aacgggaaag taattggatt cgagaagctg aattatttgc
      541 gcaaaaagat tgattcttta aaaaaacttc ctgtggaggt ggcgatttca taactatggc
      601 agacatgcag acgcagctgg aagaattaga aggcaaactc cgctatttgg cggactgtct
      661 ttgacttacc tgaaaagaaa gacaaaatag tttctttgca aaaagagtcc gagaaaccgg
      721 aattttggca ggacggagaa acttcctcgc gtctttcgca agagcttgca gaacttcgag
      781 agatagtaca ggaattcgag cggctccaaa aagactttga ggatttgaaa gaattagcca
      841 aactttcgca acgcgacgaa gacgcggccg cggaactccc cgcgatactg gaatcgctgc
      901 aaaagcgcat tgcaaaacag gaaacgcaga cattcttgtc tggcaaacac gaccgggcca
      961 atgccatttt caccattacg gcgggagccg gcgggcaaga cgccgcagac tgggccacca
     1021 tgctgcaaag aatgtacgaa cgctattgcg aaaggaaagg ctggaaggtg agtattctcc
     1081 accagtcgtt tggagatccg ggtcccgaag gccgaatcgg caccaaaaac gtatcactcg
     1141 aagttcgggg cgcgtatgcc tacgggttgc tgaaacggga aacgggcgtg catcgtttag
     1201 tacgcatatc gccgttttcc gccaaaagcc tgcgccacac gtcgtttgcc gccgtagagg
     1261 tgctcccgga aattgatgta caaaaagaaa gaggcattga actcaagccc gacgacttgc
     1321 acatcgactt tttccgtgct tcagggcctg gcggccagaa cgtaaacaag cgggaaaccg
     1381 cggttcgcat aacgcacatt cccacaggaa ttgcagtggc gtcgcaggcc gaacgctccc
     1441 agcagcaaaa caaagagaaa gcgttgcaag tgctggcttc caggttgtat caaatggcag
     1501 aacaggaacg cgccaaagaa gtggcaaaat tgcagggcca gcaagccgct atcgagtggg
     1561 ggagccagat acgctcttac gtgctgcatc cttaccgtat ggtaaaagac ttgcgaacgg
     1621 ggcttgaggt atcgaacacc gaagccgttc tctcgggcga cctcgacgaa tttattgaag
     1681 ctcaaatacg cataggaaat gattagctac agggaagtta caaaaattta tcccgacaaa
     1741 agcatcgcct tgcgcgatat atcgtttgat atcgcaaagg gagaatttgt ttcgctggtg
     1801 ggaaagtccg gagccggcaa aaccacgctg cttcggcttt tgctggccga agagcgcccc
     1861 acgcaagggc agattgtttt tgataatctt gatgtccacc aaatcaaaag atcaaaattg
     1921 cccgagttgc ggagaagcat tggcatgata tttcaggatt accggctttt ggattcaaaa
     1981 acagcggcag aaaatatcgc ctacgtaatg gaggttatgg ggttggacga ggcaaccatt
     2041 gagcgcgacg taaaggaggt gttggaaatt gtggggctca ccgaaagagc cgatcattat
     2101 ccgacacagc tctcgggcgg agagaaacag cgggttgcca tagcccgcgc gcttattcat
     2161 cgccccaggg ttatcgttgc cgacgagcct acaggaaatt tagacccgta tcataccaga
     2221 gaagttatta acctgctggt aaaaatcaac gagcttggca ccacggtgat gctggcgacg
     2281 cataacaggg aggtggtaaa tcgcctggcc aagcgggtgc ttacgttttc tgaaggcagg
     2341 ctcgtgcgcg acgaagaaaa cggtaaattt attgtattgt aacgtatgtt tttaggagta
     2401 aagcgcattg taaaatccgg aacagaaaag tttctgcgcg accgcagctc taccggagcc
     2461 gcattggtgg taatggtcat tgtgcttttt gtggtaagca cagcgtacat tctgcagggc
     2521 gtggctagtt ttttggttgc ttcgctcgaa gaaagcgtag acgtaagcgc ttacttttac
     2581 gatgcggttt cagaagaaca gattttccag gtacaagagc agttaaagac ccttgcggaa
     2641 gtaaaagaag tggagtacgt gtcgaaagac gaggcgctct cgcgttttgt ggaactgcac
     2701 cgagacgacg aagaaatcct ggagcctttg caggctttgg gcagaaaccc tttgcgcgcc
     2761 tcgctgaata tcaaggccca cgagcctggc caatacggag ccattgcaga atttctgtcg
     2821 catacctctt tttctgaaat gctcgccgat gtggatttta gcgagcgagc ccctcttatc
     2881 gagcgtttgg gagcgataac agccggtgtg cgggccacca tactggccgt aattattgcg
     2941 cttggcgcaa ttggagcgct ggttgctttt aacactattc ggcttgccat ttacagctcc
     3001 cgggaagaaa tagaagtaat gcgtttggtg ggagcttcca actggtttat caggggcccg
     3061 tttcttatcc agggggttct tgtgggcgtg gcggcaagct gcataaccat gctgtttgcg
     3121 ctgggagttg cctttggaat ttccggccag ctcgaggcgt tttccggatt taacgtaacg
     3181 caatttcttt tggggaactt ttttatgctg cttttgctac agcttatagt gggtattggc
     3241 ctgggcatta tttcttctat tgttgccatt cgccgctacc tgcaggtgta aaaaaacaga
     3301 tcctatgcta tactcataga tagttcgttg accctttaca gggaatctta ataaggaggc
     3361 ttgcgaagtg caaagtcaag acgcggtcaa gtgctcttat gtgttccctg gacagggttc
     3421 acagagcgta gggatgggtt tggaaatata caaggcatca aaggctgctc gggaagtttt
     3481 cgaggaagcc gacgatagcc tcggttttcc aatatctcga ctaatcttta acggtccgga
     3541 ggaagaactc caggataccg tcaattccca acccgccatt atggtggtga gcattgcctg
     3601 ctggaaggcg tgggaagaat tcctgggaac aaagaaaaaa aagaagcagc agggaaagcc
     3661 ctcccccgtg tcggtagcag gacatagctt aggagaatat acctcgctcg ttgttgtcgg
     3721 cgctctgcct tttgcagaag ccctttggct cgtgcgggaa cgcggccgtc tgatgcgtca
     3781 ggcatccata gagcatccgg gggcaatggc cgccatcctg ggactggacg aattcgccct
     3841 ggaacacatc tgcgccgaaa ccggcgtgga gatggccaat gttaataccg acgaccagat
     3901 agttattagc ggagaccgat tagccgtagc tcgcgctatg gacttggctt caattcgggg
     3961 agccaagaag accatttctc tgcacgtgag cggggcgttt cattcttcgt taatgtcgca
     4021 agcccgcgag ggtcttgcgg ctgcagtgga agctgtaaat ttctacgacc cacatgttcc
     4081 tgttattggc aactatacag gtacaccttt gacctctgcg gatcagctcc gcgacgaatt
     4141 gctcgaaggg ttgtgccact gcgtacaatg gcgtaactcg gtacggtaca tggcgagtgc
     4201 tggcgtaact cattttgtgg agtttggacc aagcaaggtt ttatccagct tgataaagcg
     4261 gattgacggc gacgtcgaag ctgttacctt gtcggagccg gcttccatgg aaaagtttgc
     4321 aaaggaattg cgatagaagt gcctctttag ctaggggcac ctttcttttt tgtcaaaaat
     4381 gtgttaaaat atgcgcaaat tgccgggtgg ccaaattggt aaggcatcgc gctctggacg
     4441 cgaaaactct aggttcgagc cctagcccgg cagccacgta aaattcgctc gctatgctcg
     4501 ctcatcacgt ggcaggccgc gaagaagtat aatagtagta ggctaattag tatttaagaa
     4561 ttttacgtga gccccgtggt gatttcggag aaatcacttt acggggccga gccgtgtatg
     4621 tattacgtct acctccttga aaatcaggat gataaaagtt ggtatactgg ttttacttcg
     4681 gatttgagac aacgtgtgaa agaacacaaa gaaggaaaag gaggaaggac aaccgctcta
     4741 aagagacact ggaaacttat ttattacgag gcatacctca ataagaacga tgcgcttgga
     4801 cgagaaaagt ttctcaaaag cggatctggc aggcgatacc tcaaaaagca actgaaacat
     4861 tatttgagca tttgattgag atagaggagc atgcaaaaat taattctcaa gaaaatttat
     4921 gcctcacgat aatggagaga aggcaatgca gtttgcaata aaatatgagc ggcgcagaac
     4981 cggggaaaca cccgatgacg tttctcgtga aggccgtgga tatgatcttg aatctaaaga
     5041 tagattgata gaggtgaagg ggactgacca tgttaatggc ggattggtta tttcgcttta
     5101 taagaaactt ctgaccaaac tgggcaaaga tatagtcaaa taccacattt atcttatcta
     5161 tgatatgcgt agagagccca agctcaaaat tcttacgcca gagtttatct ttgca
//
LOCUS       DASRUP010000010         9042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_444227, whole genome shotgun sequence.
ACCESSION   DASRUP010000010 DASRUP010000000
VERSION     DASRUP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444227"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFE94_00510"
     CDS             complement(<1..378)
                     /locus_tag="VFE94_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZX49618.1"
                     /translation="MAHKVHPKAFRIRQTADWFSRWFQKGTYAEALREDFLIRSYLEK
                     RLKDAGIEEIEIERFAGKVTIFLNTSRPGLIIGRGGSGIEELKREIEKIIRKDKQSRY
                     RTPKETGKREMKVEIREIKNPWDS"
     gene            complement(381..893)
                     /gene="rplV"
                     /locus_tag="VFE94_00515"
     CDS             complement(381..893)
                     /gene="rplV"
                     /locus_tag="VFE94_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZX49619.1"
                     /translation="MQSTATLQYIRIAPRKVRLVADRIRGKRVGEARLILRVTARSAA
                     EPLQKLVKSAVVSARNNFQIEESNLYIAKIAVDEGPKLKRFRPRARGRAYPIQKKTSH
                     VTLVLEEIVPSEQDQKTKATKLKGAVSQAGEGGSTTKKSTKARFRPEREQARAKSGGM
                     GKRFFRRKAI"
     gene            complement(898..1269)
                     /gene="rpsS"
                     /locus_tag="VFE94_00520"
     CDS             complement(898..1269)
                     /gene="rpsS"
                     /locus_tag="VFE94_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZX49620.1"
                     /translation="MARSLRKGPYIDEKLMKKIAGMKAGDKQAVKTWSRGATITPEMV
                     GFMFGVHNGKNFIQVAVQEDMVGHKLGEFSPTTKFVRHGGKMQKEIEQKQDEGKQPAA
                     GAPAAPLVKPAGKAAPKGVKK"
     gene            complement(1273..2061)
                     /gene="rplB"
                     /locus_tag="VFE94_00525"
     CDS             complement(1273..2061)
                     /gene="rplB"
                     /locus_tag="VFE94_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZX49621.1"
                     /translation="MKRGIFTKKKPEKSLLLHLQKHGGRGNTGRITMRHQGGGARKLY
                     RLVDFGQEQLGKTGKVQAIEYDPNRNAFLMLVEYANGAKQYAIAPHGIQAGDEITCDE
                     KTEVKMGNRMRLKNIPPGELVHNIELIPGMGGKIVRSAGTFARIDAHEGKYTQLMFPS
                     SEVRKVLSESFASLGMVSYPEFRYTRMPNAGRNRLKGRRPHVKGTAMNPVDHPHGGGE
                     GSTPVGMKYPKTPWGKPALGVKTRKSKKWTSRFIVQRRKKKKKK"
     gene            complement(2061..2432)
                     /gene="rplW"
                     /locus_tag="VFE94_00530"
     CDS             complement(2061..2432)
                     /gene="rplW"
                     /locus_tag="VFE94_00530"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZX49622.1"
                     /translation="MAALKIFKKDKREIKPEATAQPAAQPKAAVESTGDQIVRPHITE
                     KATDLGAKNQYVFVVAPGAAKNEIARSVEKAYGVKVEEVRVVNVKAKKMRLGRTKGIK
                     KGYKKAIVQVKKGQSIEILPT"
     gene            complement(2434..3063)
                     /gene="rplD"
                     /locus_tag="VFE94_00535"
     CDS             complement(2434..3063)
                     /gene="rplD"
                     /locus_tag="VFE94_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZX49623.1"
                     /translation="MKVKTYNAEGKEAGTMELPEKIFGVELNPDLVHQVMVSQQGNRR
                     QVSAHTKTRGEVSGGGRKPWPQKHTGNARHGSIRSPIWTGGGVSHGPRADRNYTKKIN
                     KKMSKKALFMVLSEKVRNDMLFMLEDMQLEGAKTKALGMLLRKLPCENKNTLIALPEM
                     NQAVIRAGNNIPSARTIQARELNVLDLLNAKYLVMPKESIEVIEKTFVT"
     gene            complement(3068..3652)
                     /gene="rplC"
                     /locus_tag="VFE94_00540"
     CDS             complement(3068..3652)
                     /gene="rplC"
                     /locus_tag="VFE94_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZX49624.1"
                     /translation="MKAILGKKIGMTQIFADEKVIPVTLVEAGPCKVTQVRTKEKDGY
                     EAIQLGYGELTKQKKITKTRKQKPFSHLKEFSGQSGVLGDVVDVSIFEEGDKVQVAGI
                     SKGKGFQGAVKRHGFSGRNASHGVKHEQRTLGSVGMSFPERVIKGRKMPGRMGVDRVT
                     VKNLKIARIDKENNILAIRGAIPGRRGTLLEIRG"
     gene            complement(3762..4112)
                     /gene="rpsJ"
                     /locus_tag="VFE94_00545"
     CDS             complement(3762..4112)
                     /gene="rpsJ"
                     /locus_tag="VFE94_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZX49625.1"
                     /translation="MPKTATRQPAEEKAAAQPKLRIKISAYDHKVIDASCKQIIDTVL
                     RQGCELVGPVPLPTEIRKYTVNRSPFVHKDAREQFEMRTHKRFIDILSPNPKVIDALT
                     NLTLPAGVDIEIKM"
     gene            complement(4120..5313)
                     /gene="tuf"
                     /locus_tag="VFE94_00550"
     CDS             complement(4120..5313)
                     /gene="tuf"
                     /locus_tag="VFE94_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZX49626.1"
                     /translation="MAGKFERTKPHVNVGTIGHVDHGKTTLSAAILHVLKLKGFVASE
                     KSVDQIDSAPEEKQRGITISLAHLEYETAKRHYAHIDAPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHILLARQVGLPYIVVFLNKVDMVDDPELIDLVESEVRELLKK
                     YDYPGDTVPVIRGSALKALEAGSPDDEAIKPVLDLMAAVDENVPEPVREIEKPFLMAI
                     EDVFSIEGRGTVATGRIERGLIKPNDEIEIVGVKATSKTVAVSVEMFNKMLDEGRAGD
                     NVGILLRGLKKEDIERGQVLAKPGSVTPHTEFDAEVYILTKEEGGRHTPFFKGYKPQF
                     YFRTTDVTGEVELPEGTEMVMPGDTISFKVKLLVPIAMEEQQRFAIREGGKTVGAGVV
                     TKISK"
     gene            complement(5358..5630)
                     /locus_tag="VFE94_00555"
     CDS             complement(5358..5630)
                     /locus_tag="VFE94_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49627.1"
                     /translation="MDLNEIKELIQKDGGKIIIVENDKPQVVVMSFEEYKANGRRTAS
                     SGPPPAPRPQPEQPFDAAQGKPAPVQMSPEPPRPQNGELSIDDLPL"
     gene            complement(5640..7745)
                     /gene="fusA"
                     /locus_tag="VFE94_00560"
     CDS             complement(5640..7745)
                     /gene="fusA"
                     /locus_tag="VFE94_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZX49628.1"
                     /translation="MAREYPVEKYRNIGIIAHIDAGKTTSSERILFYTGISHKIGEVH
                     EGAAIMDWMVQERERGITITSAATTCFWVPTYLQNEQANKYRINIIDTPGHIDFTAEV
                     QRSLRVLDGAVVIFDGVAGVEPQSETVWRQADKFGVPRICFINKLDRTGASFEKSLGS
                     IVKKLTKNAVALQIPMGEENAMKGVIDLLEMKAVYFDGDFGQTIRKEEIPAELQERAK
                     EWREKAVEKIAAEDEALMNKYLGAQEVTLPELKKTLRKASLQGKLVPVFVGSALKNMG
                     VQLVLDGVIDYLPSPVDVPAIEGVDPKTGESIPRKPADTEPFAALAFKVASDPYVGTL
                     TYFRVYSGTLQKGSYVLNTTTGEKERISRILRMHAAEREEVDELYAGDIAATVGLKAT
                     STGHTLCAENAPIALEQISFPEPVISIRIEPKTKADQEKMGLALRKLAGEDPTFRVTS
                     DLETGETIISGMGELHLEIIVDRMKREFGVGASVGRPQVAYKETIRKDAVAETKYVKQ
                     SGGRGQYGHVLLRVEPKGEGEGFEFVDAIKGGAIPKEYIPAVEKGVREAMDKGVLAGY
                     RLVDAKVTLKDGSYHEVDSSELAFKIAGSMAFQEAARKANLVLLEPVMHVEVTIPSDF
                     LGDVIGDLSSRRGRIEETDDLLDMKVIQAKVPLSEMFGYATNLRSLTEGRGTFTMEFE
                     RYEEVPANITQEVIEGKRR"
     gene            complement(7751..8218)
                     /gene="rpsG"
                     /locus_tag="VFE94_00565"
     CDS             complement(7751..8218)
                     /gene="rpsG"
                     /locus_tag="VFE94_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZX49629.1"
                     /translation="MPRGNFRKPAVDPDSVYGDVMVSKFVNQVMERGKKSAARKIVYG
                     AFDLVKTQSKQDPLEVFQKAIENSSPLLQVRSRRVGGATYQVPMEVKADKRMSLAMKW
                     ILQAAKSKKGKPMKEKLAEELLAAYKNEGSAVKKKEDMHRMAEANRAFAHFAW"
     gene            complement(8222..8632)
                     /gene="rpsL"
                     /locus_tag="VFE94_00570"
     CDS             complement(8222..8632)
                     /gene="rpsL"
                     /locus_tag="VFE94_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZX49630.1"
                     /translation="MPTIRQLIKKPRKKAGKKDKTPALSFGFRSQKNRIVRYPSSFKR
                     GVCVRVFTTTPKKPNSALRKVARVRLSNGMEVTAYIPGVGHKLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGVLDAGGVEGRKQERSKYGTKRGK"
     gene            complement(8674..>9042)
                     /locus_tag="VFE94_00575"
     CDS             complement(8674..>9042)
                     /locus_tag="VFE94_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZX49631.1"
                     /translation="RDQKFGSAKVSFAGPAANLAIALVFGLLLRFFSGPIAALSPELI
                     GMLVIIVQLNLILAIFNLIPIPPLDGSHILFDFLPASFDGLKRFLMQYGFFALIFFAF
                     FIFPMFIPVVHWLFRFLVGF"
BASE COUNT         1923 a         2473 c         1931 g         2715 t
ORIGIN      
        1 cgaatcccag ggattcttga tttccctaat ctccaccttc atctcgcgct ttccggtttc
       61 tttgggtgtc cggtatctgc tttgtttgtc tttccggata attttttcga tttccctttt
      121 caattcttca atgccagaac cccctcttcc aataatcaaa cccgggcggg acgtattcaa
      181 aaaaatcgtt accttccctg caaaacgctc gatttcaatc tcttcaatgc cggcatcctt
      241 caagcgcttt tccaaatagg aacgaataag aaaatcctcg cgcagggctt ctgcgtacgt
      301 acccttttga aaccaacgtg aaaaccaatc tgccgtttgt cgaattcgaa atgcttttgg
      361 atggactttg tgtgccatac ttatattgct tttcttctaa aaaacctttt tcccatgccg
      421 cccgacttcg cgcgtgcctg ctctctttcc ggtcgaaatc tcgctttcgt agattttttt
      481 gtggtgctcc ccccttcgcc tgcctgtgaa actgcgccct ttaacttcgt tgctttcgtt
      541 ttttgatcct gctcactcgg cacgatttct tcgagcacca gcgtaacgtg ggaagttttc
      601 ttttgtatgg ggtatgccct tccgcgggcg cgtggccgaa aacgcttaag ttttggccct
      661 tcgtctacgg cgatttttgc tatatataaa tttgactcct ctatttgaaa attgttgcgg
      721 gctgaaacca cagccgactt caccaatttt tgcaaaggct ctgccgcgct tctggcggta
      781 acgcgtaaaa tgagccgggc ttctcccact ctctttccgc gaatccgatc ggccactaat
      841 ctgacttttc ggggcgctat tctaatatat tgaagtgtag ctgtgctttg catatgccta
      901 tttctttact ccttttggcg cagccttccc cgccggtttc acaagaggcg cagccggagc
      961 tcccgccgcg ggctgctttc cctcgtcctg tttttgttct atttcttttt gcatctttcc
     1021 gccatggcga acaaatttcg tggtaggaga aaattctccc agcttatggc ctaccatgtc
     1081 ttcctgcacc gccacttgaa taaagttttt cccgttgtgc acgccaaaca taaacccaac
     1141 catctcgggt gtaatcgtag ctccacgcga ccaggttttc accgcctgct tgtcgccggc
     1201 tttcattccc gcaatctttt tcatcagttt ctcatcaata tagggtcctt ttcgtaaact
     1261 tcgtgccata tgttatttct ttttcttctt ctttcttcgt tgcactataa atctgcttgt
     1321 ccactttttc gattttcttg ttttcactcc caaagccggc ttgccccacg gggttttggg
     1381 atatttcatt cccaccggag tgcttccttc acctcctcca tgcggatggt ctaccggatt
     1441 cattgccgtg ccttttacgt gcggccttct gcctttcaac ctgttgcgcc ccgcgtttgg
     1501 cattcttgtg tagcgaaatt caggatacga caccattccc aaagaagcaa acgattcaga
     1561 caatactttt cgcacctcgc tcgaagggaa catgagctgc gtgtattttc cttcgtgggc
     1621 atctattctt gcgaaagttc ccgcagatct cacaattttg ccgcccatgc cggggatcag
     1681 ctcaatgttg tgcaccaact cgcccggagg aatattcttg agccgcatgc ggtttcccat
     1741 ttttacctcg gttttttcgt cgcaggtgat ttcgtccccc gcttgaatgc catgaggagc
     1801 aatcgcgtat tgcttggctc cattggcata ttccaccagc attaaaaacg catttctatt
     1861 ggggtcgtat tctatcgcct gcaccttgcc ggttttcccc aattgttcct ggccgaaatc
     1921 aaccagccgg tagagctttc gggctcctcc accctgatgg cgcatggtaa tgcggcctgt
     1981 attgcctcgc ccgccgtgct tttgcaaatg cagcagcagg cttttttccg gcttcttttt
     2041 tgtaaaaatt ccgcgtttca ttatgtcggt aatatttcaa tgctttgccc cttcttaacc
     2101 tgcacaatcg ccttcttgta tcctttcttg attccctttg ttcttcccaa acgcattttc
     2161 tttgctttta cattcaccac tcttacctct tctaccttta ctccgtatgc tttttccaca
     2221 gaacgggcga tttcgttttt agccgcgcca ggagctacca caaacacata ctggtttttt
     2281 gctcccaaat cagttgcttt ttcagtaatg tgcgggcgta ctatctggtc tcccgtgctt
     2341 tccaccgcag cttttggctg cgcggcaggt tgcgcggttg cttccggctt aatttctctt
     2401 ttgtcttttt taaaaatctt tagcgcagcc atactatgtt acaaatgtct tttctataac
     2461 ctcaatcgat tcctttggca tcaccagata tttggcgttg agcaaatcca acacattcag
     2521 ttctcttgct tgtatggttc tggccgaagg aatgttattt cccgcgcgga taaccgcctg
     2581 attcatttct ggcagcgcga ttaaggtgtt cttgttctcg cagggcaatt ttcgcaacaa
     2641 catgcccaaa gccttggttt tagcaccttc caattgcata tcttccagca tgaagagcat
     2701 atcgtttctg actttttcag aaagcaccat aaacaaagct ttcttgctca tttttttatt
     2761 aattttcttg gtgtagttac ggtctgctct tggcccgtgc gacacgccgc cccccgtcca
     2821 aatgggtgat cttatagagc catggcgcgc gtttcccgtg tgtttttggg gccagggttt
     2881 tcttccgccc ccggaaactt cgcctctggt tttggtatga gctgaaacct gcctccggtt
     2941 tccctgctgt gaaaccatta cctgatgcac caaatcaggg tttagctcaa ccccaaaaat
     3001 cttttccggc aattccatgg ttcccgcctc tttgccttct gcgttgtatg tttttacctt
     3061 cataaaacta tccgcggatt tccaaaaggg tgccgcggcg gcccgggatg gctccccgta
     3121 tggccaaaat attattctct ttatcaattc tcgcaatttt caaatttttt accgttaccc
     3181 tatctacgcc cattctaccc ggcatttttc ttcctttaat aacgcgctct ggaaacgaca
     3241 ttcctaccga gcccagggtg cgctgttcgt gctttactcc atgactggcg tttctgccgg
     3301 aaaatccatg ccgctttacc gctccctgga atccctttcc cttggaaatg cccgcaacct
     3361 gcaccttgtc cccttcttca aatatagaca cgtctaccac atctcccagg acgccgcttt
     3421 gcccggaaaa ctctttcaaa tgggaaaagg gtttctgttt tcgtgttttg gtaattttct
     3481 tttgtttggt taattccccg tatcctaact gtattgcttc gtatccgtct ttctccttgg
     3541 ttcttacttg ggtaactttg caaggcccgg cctcaaccaa ggtaacaggt attacctttt
     3601 cgtcggcgaa aatctgcgtc atgccaattt tttttcctaa tattgctttc ataaaaacaa
     3661 ccccgactaa cccggggccg taaagccttg ttttcagggt cggtccctta gattttaagg
     3721 cgtgaataga acactattca tcaaataacg ttataccaca actacatttt aatttcaata
     3781 tccacccccg caggcaacgt caggttggtt aaagcgtcaa ttactttcgg gtttggactc
     3841 aaaatgtcta tgaagcgctt gtgcgtgcgc atctcaaact gttctctggc gtctttatgc
     3901 acaaacggcg aacggtttac cgtatatttc cggatctctg tgggtaacgg caccggcccc
     3961 accagttcac atccttgtcg caacaccgta tcaataattt gtttgcaaga agcgtctatg
     4021 accttgtggt cgtaggcgga aattttaatc ctcaactttg gctgtgctgc tgctttttct
     4081 tccgccggtt ggcgggttgc ggtctttggc atgtgctttt tatttgctga tcttggtcac
     4141 caccccggcg cccacggtct ttccgccctc gcgaatggca aagcgctgct gttcttccat
     4201 tgcaataggt accaacagtt ttaccttaaa gctaatggtg tcgcccggca ttaccatttc
     4261 ggttccttcg ggcaactcca cctcgcctgt cacatcggtg gtacggaagt aaaactgggg
     4321 cttatacccc ttaaagaatg gggtgtggcg cccgccttcc tctttggtta aaatatacac
     4381 ttcagcgtcg aattcagtgt gaggagttac ggaacccggc tttgccaata cctggcctct
     4441 ttcgatatct tctttcttta agccccggag caaaatccct acgttgtccc ctgcccttcc
     4501 ctcgtcgagc attttgttaa acatctccac gcttactgct acggttttgg aagttgcttt
     4561 cacgccgaca atttctattt cgtcgttggg cttaatgagt cctctttcaa ttcttccggt
     4621 tgccacggtt cctcttcctt caatggaaaa cacgtcttca atagccatca agaacggctt
     4681 ctcgatttcg cgaacgggct ctggtacgtt ttcatccact gctgccatca aatccaaaac
     4741 cggtttaatg gcttcgtcgt ctggcgaacc tgcctccaaa gctttcaggg cggacccacg
     4801 aataacaggg accgtatcac cggggtaatc gtatttcttg agcaattccc gaacttcaga
     4861 ctccaccagg tcaatcaatt cagggtcgtc caccatatct accttgttca aaaacaccac
     4921 aatataaggc aagcctacct ggcgggcaag caaaatatgc tctctggttt gaggcatggg
     4981 gccgtctggg gcagacacca ccaaaatggc gccgtccatt tgggcggctc ccgtaatcat
     5041 gtttttaatg tagtctgcgt gtccgggggc gtcgatgtgc gcatagtggc gctttgctgt
     5101 ttcgtattcc aagtgcgcaa gagaaatggt aattcctctc tgcttttcct cgggagcaga
     5161 atcgatttgg tcgacgctct tttctgatgc tacaaaaccc ttcaacttta atacgtgaag
     5221 aattgcagcc gacaacgtag ttttcccgtg gtcaacgtgg ccgatagtac cgacgttcac
     5281 atggggcttt gttcgttcga attttcctgc catagtgcaa tgagttaacg atattagtta
     5341 gtttattatt tatataccta aagtggcaaa tcgtcaatac ttagctcgcc attctgcggc
     5401 ctcggaggtt ccggagacat ttgcaccggg gcgggcttgc cctgagccgc gtcgaagggc
     5461 tgctcgggct ggggcctggg agccggcggt ggccccgatg atgccgtcct tctcccgttt
     5521 gctttatatt cttcaaacga cattaccact acctggggct tgtcgttctc tacgataata
     5581 atttttcccc cgtccttctg tataagttct ttaatttcat tcagatccat aggtgtatct
     5641 taacggcgtt ttccctcgat tacttcctgc gtaatgttcg caggaacctc ttcatatcgt
     5701 tcaaattcca tggtaaaggt tccgcggcct tccgtcaagg aacgaaggtt tgtggcatac
     5761 ccgaacattt ccgacaacgg aactttggcc tgaataacct tcatgtccaa aaggtcgtcg
     5821 gtttcctcaa tgcgacctct gcgcgaagaa aggtcgccga ttacgtctcc cagaaagtca
     5881 gaaggaatag taacctcgac atgcataaca ggttccagca gcaccaaatt cgcttttctg
     5941 gccgcttcct gaaaggccat ggaacccgct attttgaacg caagctccga agagtctacc
     6001 tcgtggtagg aaccgtcttt caatgttact ttggcgtcta cgagccgata cccggcaagc
     6061 actcccttgt ccattgcttc ccgtaccccc ttttctaccg caggaatgta ttctttggga
     6121 atggcacctc ctttaatcgc gtctacgaat tcaaagcctt ctccttctcc tttaggctca
     6181 acgcgcaaaa gcacatggcc atactgcccg cggcctccgg attgttttac atacttggtt
     6241 tcagcgacgg catctttgcg gatggtttct ttatacgcca cctgaggcct tcccacgctt
     6301 gcccccacgc cgaattcccg cttcatgcgg tctacaataa tctccaaatg aagctccccc
     6361 atgccggaaa taatggtctc cccggtttcc aaatcggaag tcacgcgaaa cgtggggtcc
     6421 tcacccgcta atttgcgcaa agccaagccc attttctcct ggtcggcttt cgttttgggt
     6481 tcaatgcgaa tagaaataac gggctcggga aaagaaatct gttctagcgc aataggggcg
     6541 ttttctgcgc aaagcgtatg cccggtgctg gtggccttca aacccaccgt ggctgcaatg
     6601 tcgcccgcat acaactcgtc tacttcttct cgttcagccg cgtgcatgcg caatatgcgg
     6661 ctaatacgct ctttctctcc ggtagttgtg ttcaatacat aagacccttt ttgcaaggtg
     6721 ccagagtaca cgcgaaagta ggttaatgtc ccaacatagg ggtcggacgc cactttaaaa
     6781 gcaagtgccg caaagggttc ggtatctgca ggctttcggg gaatggactc tcctgttttg
     6841 ggatcaacac cttcaatggc gggcacgtcc accgggcttg gcaaataatc aatgacgccg
     6901 tcgagcacga gctgcacacc catatttttc agggcagaac ccacaaatac cggcaccagc
     6961 ttgccttgga gagatgcctt gcgtaaggtt ttcttaagtt ccggcaacgt cacctcctgg
     7021 gcgcccaaat atttgttcat taaggcttca tcttccgcag caattttctc caccgctttc
     7081 tcgcgccatt cttttgccct ttcctgcagt tctgccggaa tttcttcttt tcttatcgtt
     7141 tggccgaaat ctccgtcgaa gtacacggct ttcatttcca ataaatcaat tactcctttc
     7201 atggcgtttt cctctcccat aggaatttgc agagcaaccg cgtttttggt taatttcttt
     7261 acaatagaac ccaggctttt ttcaaaagaa gcgccggtgc ggtcgagctt gttgataaaa
     7321 caaatgcggg gaacaccaaa cttgtctgcc tggcgccata cggtttccga ttgcggctcc
     7381 accccggcca cgccgtcgaa aataacaacc gctccatcca atacgcgaag cgatcgctgt
     7441 acctctgcgg taaaatcaat gtggcctgga gtatctataa tattaatacg gtatttgttt
     7501 gcctgttcgt tttgcaaata ggtgggtacc cagaaacacg tggtggcggc ggaggttatg
     7561 gtaattccgc gttctctttc ttgcaccatc cagtccataa ttgcggcccc ttcgtgcact
     7621 tcgccgattt tgtgcgaaat tcccgtgtaa aacaaaatgc gctcagacga cgtggtcttc
     7681 ccggcatcaa tatgggcaat aatcccgata tttcgatatt tttcaacagg atactcgcga
     7741 gccatatatt ttaccaggcg aaatgagcga atgcgcggtt ggcttcggcc atgcggtgca
     7801 tgtcttcttt cttttttact gcagaaccct catttttata tgccgcaagc agctcttctg
     7861 ccaatttttc tttcatgggc ttgcctttct ttgactttgc cgcttgcaaa atccatttca
     7921 tggccaggct catgcgctta tcagccttta cttccatggg cacctgatag gtggctcctc
     7981 ccacccttcg ggagcgaacc tgcagcaagg gcgagctgtt ttcgattgct ttttgaaaca
     8041 cttcgagggg atcttgttta gactgggttt ttaccaaatc aaaagcgccg tacacgattt
     8101 ttcgtgcggc cgattttttg ccccgttcca ttacctggtt cacaaacttg gaaaccataa
     8161 cgtctccata cacggaatcg gggtcaactg ctggttttcg gaaatttcct cgcggcatat
     8221 gttattttcc tcttttggtt ccgtatttgg aacgctcttg ctttcggcct tctacgcctc
     8281 ccgcgtccag tacgccgcgc acaatgtggt atcttacgcc tggcaaatcc tttactcttc
     8341 cgccgcgcaa aaggacaacc gagtgctcct gcaacttatg tcccaccccc gggatatagg
     8401 cggttacttc catgccgttg gacaacctta ctcttgccac tttccgcaag gcggagtttg
     8461 gctttttagg cgtagtggta aacactctca cgcacactcc ccttttaaac gaagaaggat
     8521 agcgcacaat acggtttttc tgcgagcgaa aaccaaaact caaagcaggc gttttgtctt
     8581 tcttgcccgc cttttttctt ggttttttga taagctgccg aatagttggc atgtcgcaaa
     8641 taatgtatca aattaactct tttttgtcaa tcgctaaaac cctacgagaa accggaaaag
     8701 ccagtgcacc accggaataa acatggggaa aataaaaaat gcaaagaata tgagcgcaaa
     8761 gaatccgtac tgcatgagaa agcgctttag tccgtcgaaa gacgcaggca gaaaatcaaa
     8821 aagaatatga gaaccgtcta agggcggaat gggtatcagg ttaaaaatgg caagaatcaa
     8881 attgagctgc acgataatca ccagcatgcc aatgagctct ggggacaggg cagcaatggg
     8941 gccgctgaaa aaccgcagca aaagcccgaa cacaagggca atggcaaggt ttgccgcagg
     9001 gcccgcaaag cttactttgg cggaaccaaa cttctgatcg cg
//
LOCUS       DASRUP010000011         3722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_836289, whole genome shotgun sequence.
ACCESSION   DASRUP010000011 DASRUP010000000
VERSION     DASRUP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836289"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFE94_00580"
     CDS             complement(<1..188)
                     /locus_tag="VFE94_00580"
                     /inference="COORDINATES: protein motif:HMM:NF028434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZX49632.1"
                     /translation="MKKVLLVVSEKGYTWDEVIYPYLEFQKKGWQVAFATPVGGTPKV
                     DPVSIIVRPVLQMFGYGTA"
     gene            complement(188..454)
                     /locus_tag="VFE94_00585"
     CDS             complement(188..454)
                     /locus_tag="VFE94_00585"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZX49633.1"
                     /translation="MLGRVARGQAKNHMAVYLMNVYGNKENEQWFGEELEDWEKQHGK
                     RVDMGKSCVRFKKLEDLPVELIAKAIVKTPVHKFIVMYEESRKR"
     gene            604..2391
                     /locus_tag="VFE94_00590"
     CDS             604..2391
                     /locus_tag="VFE94_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HZX49634.1"
                     /translation="MRLGTTFTQLFSLDLRALAALRVGLAFLILTDLLNRVRYLTAHY
                     TDQGVFPGAATDQFYQNPFFFSFHSLNGSWEFQAFLFLLAGVFACMLLVGFHTRLATV
                     LSWMLLASLQTHNPLILQGGDILLRLTLFWAIFLPLGARYSVDSIRKPAFLETNIFSS
                     VASAAYILQIAFVYFFTALLKSGSQWVLEGSAVYYTLSIDQLVSPFGNFLLSFPVLLQ
                     YLTFSILALEFLAPFLLVAPFKTYQFRLAAALALIAFHVGLATAMHLGPFPWIGIIST
                     LALLPPPFWNFLSRKRIANKKQNLVLYYDGECGFCQRTVQYIKTFFLIPKLQILPASQ
                     EESILRDMLRYNSWVVVDRQGKRHYKFDGVTVVVKACPFLWFFAPLLRIPFIFRAGTN
                     IYEYIASHRKTQCFPFSHPSPAPNQTVKSRIAIALSGSIVFALIIYVFLWNLGNMFPS
                     AYFLPPESKNLAYSLRLDQLWNMFSPFPYTDDGWYVVVGTLKNGTTTDLFQKVSPVSW
                     EKPRVVADTYDGERWRKYMMNLWYQALAPYRVYYAQYLCHSWNKTHNSQEQLESVEVT
                     YMLERTLPGFQYSKPQPVSLLQYQCLLPV"
     gene            complement(2437..2859)
                     /locus_tag="VFE94_00595"
     CDS             complement(2437..2859)
                     /locus_tag="VFE94_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49635.1"
                     /translation="MMLNKEAIINIKRPVLTWLAIGAGAIAVLLLAWWALSGGGFDLD
                     NRFPLGSEQGREQTRQEGTATSEAELNAALEQAKQRLAVLQIRMETEQVSDEMIEEVQ
                     DIRVTLAAAYEDANAEAKEKWRKLDEGLEDLEQNLRKS"
     gene            complement(2921..>3722)
                     /locus_tag="VFE94_00600"
     CDS             complement(2921..>3722)
                     /locus_tag="VFE94_00600"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX49636.1"
                     /translation="TRELALQIDQEFRLFASQLSLYSTVCVGGESIGMQIRQLRRQNH
                     FIIGTPGRVIDLIKRNVISLNTIHTVVLDEADRMLDMGFIADIRFILAGIRKDRHTML
                     FSATMSPRIKGLISDFLRDPVMVSVKTHETPNNIHQDVVSCKNENKIDLLSKFLADQE
                     FKKVLVFGRTKHGVERLCKELSRRGVKVDSIHSNKSHGQRQYALRNFKENRLQALVAT
                     DIAARGLDIEGVTHVINYELPATYEDYVHRIGRTGRGANKGKALTFVD"
BASE COUNT          848 a         1021 c          744 g         1109 t
ORIGIN      
        1 gccgtgccgt atccaaacat ttgcaacacc ggccgcacta taatacttac agggtctact
       61 tttggcgtgc cccccacggg cgtggcaaaa gcaacctgcc accccttttt ttgaaactcc
      121 aggtagggat atataacctc gtcccacgta tatccttttt cacttaccac aagcaaaact
      181 tttttcatta tcgtttacgg gattcttcgt acatgacaat aaacttatga acaggtgttt
      241 tgactattgc ttttgcaata agctcaacgg gcaagtcttc taacttttta aatcgcacgc
      301 acgattttcc catatctact cttttcccat gctgcttttc ccagtcttcc agttcttcgc
      361 cgaaccattg ctcgttttcc ttattaccat acacattcat taagtacact gccatatggt
      421 ttttcgcctg tccgcgggca accctgccaa gtattctgta accgttgcag ctttactctg
      481 taccatatag cttgattatc tcttaaaaaa gctttttgtg aaatacccta ttacttcttt
      541 cattggccgg catccatatc ttctgaaaaa tctgatatgc tataactaat tattaaaaat
      601 accatgagac tcggaacaac attcacccag ctattcagtc tagaccttcg cgcccttgcc
      661 gctctgcggg ttggtttggc ttttcttatt cttaccgatc tactcaatcg cgtacgctac
      721 ttaaccgccc actacactga tcaaggggtg ttcccaggcg ccgcgacaga tcagttctac
      781 caaaaccctt tctttttttc cttccattca ctcaatgggt cgtgggaatt ccaggctttt
      841 ttgttcctgc ttgccggcgt gtttgcctgc atgcttttgg tcggatttca tacgcgcctt
      901 gccactgtac tttcctggat gcttctcgct tctctccaaa ctcacaaccc gcttatcctg
      961 caaggtgggg atattctttt gcgacttacc cttttctggg ctatatttct ccctttggga
     1021 gctcgctatt ccgtagacag catacgaaaa cctgcatttc tagaaaccaa tattttttct
     1081 tccgtagctt ccgcggccta catactacaa attgcatttg tatacttttt taccgccctt
     1141 ttaaaaagtg gcagccagtg ggttctggaa ggaagcgcag tatactatac ccttagcatc
     1201 gaccagcttg tttccccttt tggtaacttt cttctttctt ttccggttct cttgcaatac
     1261 ctcacttttt ccattctcgc tcttgagttt ctagctccct ttctcctggt tgctccgttt
     1321 aaaacatatc aatttcgcct ggcggccgcg cttgctctta tcgcattcca cgttgggctg
     1381 gcaacagcaa tgcatctcgg accgtttccg tggataggga taatatccac acttgcgctc
     1441 ttgccgcccc cattctggaa cttcctgtct cgaaaacgta tcgcaaacaa aaaacaaaac
     1501 ctcgttctct actacgacgg agaatgcggg ttttgccaac gaacagtcca atacataaaa
     1561 actttttttc ttattcccaa gctccagatt ttgccggcca gccaagaaga atctatattg
     1621 cgcgacatgc tgcgctataa ctcgtgggta gtggttgacc gacaaggcaa acgccactac
     1681 aaattcgatg gggttactgt tgtcgtaaaa gcctgccctt tcctttggtt ttttgcccca
     1741 ctccttcgca ttccctttat ttttcgcgca ggcacaaata tctatgaata tatcgcctct
     1801 caccgaaaaa cccagtgttt tcccttttct cacccatctc ctgcgccaaa ccaaacagta
     1861 aaaagccgga tcgctatcgc tctttccggc tcaatcgtct ttgcgcttat tatatatgtt
     1921 tttctttgga accttggcaa tatgttccct tcggcatact ttttaccgcc agagtcgaaa
     1981 aaccttgctt attccctgcg gctcgaccag ttatggaaca tgttctctcc ctttccttac
     2041 acagacgacg gctggtatgt ggtagttggg actctcaaaa acggaacaac cactgatctt
     2101 tttcaaaagg tctctccggt ttcctgggaa aaaccacgcg tggtagctga tacctacgac
     2161 ggggaacgct ggcgcaaata catgatgaac ctttggtatc aagcgcttgc gccctaccgg
     2221 gtatactacg cccaatatct ttgccattcg tggaacaaaa ctcacaactc tcaagaacag
     2281 cttgaaagcg tagaagtcac gtatatgctt gagcgcacac tgcctggctt tcaatactca
     2341 aaaccacaac cagtatcgct tttgcagtac caatgccttt taccagtatg acgttccaag
     2401 cggtttcttg ccgcctcaac cactatccca ttatactcaa ctcttgcgca ggttctgttc
     2461 caaatcttcg agcccctcgt caagtttccg ccatttttct ttggcctcgg cgtttgcgtc
     2521 ttcataggca gccgccagag taaccctaat atcctgtacc tcctctatca tttcgtctga
     2581 aacttgctct gtttccattc ttatctgaag aacggcaagc cgttgctttg cctgctctaa
     2641 agcggcgttc agttctgctt cgcttgttgc ggttccctct tgcctggttt gctcccggcc
     2701 ctgctctgaa cccaggggga accgattgtc caggtcaaaa ccgcccccgc tcaaagccca
     2761 ccatgcgagc aaaagaaccg caattgctcc ggctccaatg gccagccacg ttaaaacagg
     2821 cctttttata ttgattattg cttctttgtt taacatcata cttatagaaa agacgaacaa
     2881 aaaaccaatt tgtaacagtc ctatcttacc actcaaaaaa ctaatctaca aacgtaaggg
     2941 ctttcccttt gtttgcgccg cggccggtgc gaccgatgcg gtgcacatag tcttcgtagg
     3001 tggcgggcag ctcgtaattg ataacgtggg ttacgccttc aatgtcgagg ccgcgcgcgg
     3061 caatatcagt ggcaacaagc gcctgcaagc ggttttcttt aaagttgcgg agcgcgtatt
     3121 gccgctggcc gtgcgatttg ttgctgtgaa tagaatccac ttttacgccc cggcgagaaa
     3181 gttccttgca caggcgttcc acgccgtgct ttgtccggcc gaacaccagc acttttttaa
     3241 attcctggtc tgccaaaaat ttggaaagca ggtcaatttt gttttcgttt ttgcagctta
     3301 ccacgtcctg atggatgttg tttggcgttt cgtgcgtttt taccgaaacc atcacggggt
     3361 cgcgcaaaaa gtcgcttatt aaacctttaa tgcggggcga catggttgcg gaaaacagca
     3421 tggtatgccg gtctttacgg atgccagcca gaataaaccg gatgtcggcg ataaatccca
     3481 tgtccaacat gcggtctgcc tcgtcgagca ctacggtatg tatcgtgttt aacgaaatta
     3541 cgttgcgctt gataaggtca ataacgcgtc cgggagtccc gataataaaa tggttttggc
     3601 gccggagctg gcggatttgc atgccaatgc tttcccctcc cacgcatacg gtggaataca
     3661 aagaaagctg gctcgcgaac aagcgaaact cctggtcgat ttgcaaggca agctcgcggg
     3721 tg
//
LOCUS       DASRUP010000012         6843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_850382, whole genome shotgun sequence.
ACCESSION   DASRUP010000012 DASRUP010000000
VERSION     DASRUP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850382"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..954)
                     /locus_tag="VFE94_00605"
     CDS             complement(160..954)
                     /locus_tag="VFE94_00605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZX49637.1"
                     /translation="MRRSLSEFASLGAIKHTIYIPSVVQQIANWWPFLLNYIGVKNYG
                     AVYRLRNGIEIETGEGVDAATIAVVFVKKAYGDAKDNTTIIDIGANIGVYAVYAAGTS
                     KNTKVYAYEPLPRSYSLLLKNMQRNRLQKFIFPFRVGVAGSRGRRQLFLANGSPFHSF
                     YRTQEGKGHVEIGCITLQEIFDENNIEQCDVLKIDCEGAEFEILLNTPLTYLAKIQEI
                     RMECHDVGGGELQRARLLAYLKAGGFRLVRFTQDTPTSSTWWLQKA"
     gene            complement(1020..1526)
                     /locus_tag="VFE94_00610"
     CDS             complement(1020..1526)
                     /locus_tag="VFE94_00610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX49638.1"
                     /translation="MKVLDLGCGDRKKVIEGAQVIGIDKIAMPGVDAVHDLNVFPYPF
                     GNNEFDQIICDDVLEHLDDIVAVVEELHRITKPGGEIIISVPHFSSDNYYTDITHKHP
                     FSSRSFDFFDPRFQNNVHQFYSKARFRVEKKYIGFSEVLPSGKKHFPNVWKILGVEYL
                     ANRFPRHY"
     gene            complement(1550..2863)
                     /locus_tag="VFE94_00615"
     CDS             complement(1550..2863)
                     /locus_tag="VFE94_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZX49639.1"
                     /translation="MNYFKLGDIIWQRVFGYPMGPEAKRFWQNVSFVSVGTLGASVLG
                     FFVMIVAGRILGPFEYGKANLVFSIGSVLLLVMTLGFPYALVKNIVQEREVKRIVPSL
                     FVITIAWAFAMTLVLLLVSHPLSRLLRVDLLVFLFAIAFALTHVFVVVSKSLFQGLQQ
                     FGRFAAFHVLSALFFVLFFFGFANLGEDKSFTSLIFAEILRWLFPVLIGFFLLRSLIF
                     PMRIHWKNTKTLLSFSIWVFLGLTPGYLFGYIDRLMIHYFLGSTAVGFYSAYVLSSTV
                     MLERGLGVFLAVFFPYSSAAPHKKILFRKINRFMAFLAVPATGLLYGIIAFALLLFGP
                     EYEFNVVLILLFSVNGFLFFVSSIYSWLLFSMEIKTVRFGAFIPWAGLVINVFCNTLL
                     IPVIGLYGAVVATIAAFLFVIVSTNIYFATMKKEVPEQKHVETSR"
     gene            complement(2872..4941)
                     /locus_tag="VFE94_00620"
     CDS             complement(2872..4941)
                     /locus_tag="VFE94_00620"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZX49640.1"
                     /translation="MFLVQQALEILFKILVGVSLLLPLLAGSAYTVPALGPKVFFFQP
                     IVVALAGIYALLSLVDFQKYRIRLNILSWAILLWGASLLVSTLFGVDWYRSLWSTSLR
                     LTGVVFLLHCIAYYFLLGQVLQKEDWRWVLRVFLGGGILAILFALIFEVGFPQALYQK
                     GEEVLRAASTLGNAAFFGGFAMALVFVAYFLLAQEKQGRWFSFALAGAVVSLFGVLAS
                     GTRGAFLGLACGLLFLCVSLAVLWKRAGKEGRKHIFGTIGFMLLPLFIFGGVSLVFVV
                     QNAFSVADVPAVERFVGQGATATLHTRLLAWNVAFEAWKEKPLFGWGPGNFAYAFHAY
                     YPPEILQYGEPETWFDVAHNVLLEALATQGITGLFALLGIYGAAAFLLWQGYRQKQIE
                     PAVVGVAGAFLVAHLVFGLFLFDIPTSYISLFLVLAFMGSQIHLAKEAAQKPKRLGKK
                     VFLAGAIVLVTTALLAGYLVPVGVDRLFAKAKDNLPDNVPQAIAWYERAERFPSPYTR
                     STKAYVLNQTMPVLYTLVEQGNKKDAATLFLFAEKELQELQRQEPLEVRHGLLFGHIY
                     FAGAELFQEQEVLGKAERVWGEALLKSPERQEFQYWLALAKFRLGKFQEGFLLLEGVV
                     SSNADIQKGWMLLLCVNRETTPFKEVQKQMKNYLERGTSSENEREIIRFLLEPECQKG
                     VSSFPSK"
     gene            complement(4946..5027)
                     /locus_tag="VFE94_00625"
     tRNA            complement(4946..5027)
                     /locus_tag="VFE94_00625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4991..4993),aa:Leu,seq:tag)
     gene            complement(5177..6688)
                     /locus_tag="VFE94_00630"
     CDS             complement(5177..6688)
                     /locus_tag="VFE94_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49641.1"
                     /translation="MKTLFLLLLVICALGIFGCTTTTTTTRRWVPAADAAVATPTPQP
                     APTATPVPPPSPESISTVGFEKIWEQDFEDEHIPSPGYGEPYKAIAPFAGGMVEWGSI
                     HHFDSSLRQSALGAKSIDLNGVAGPGAWAWEFPTIPGEPFSVSYRLAGNTANGPLEKR
                     GRVEIIQIDSEKVEPFSFDIRGKSLFNMGWVERELPFIARGERTIVRFVSDTPGLFGP
                     SIDSVQVKGPKHVAEELRGKYAPIETPVATPVPGVAPDATPMPTPTAIPTPTLIPTPT
                     PQPVAPGTQGIMLYRNNSKRVSGNLPPKPHTDEQVKVCHQFEENGVAVNKTHGLGDRV
                     VRGGQMFPDCTYFAFDGGEAFESEFFASFDAHDKCFDAVSRNVYRRRQATGEEEWMGK
                     QVLVSGNCDNETGFEVANPYTLFGNPVYRPEEGTITVTLIKAVDDNQSYTVFSSLVCP
                     FGYTAAYRTREEGEFITDVGSSFETDASSLELEAKLTGLSTPSNIRQCALSLK"
BASE COUNT         1988 a         1815 c         1458 g         1582 t
ORIGIN      
        1 cgccgttcac ggaatccaca actcccttgc ccttgggctg gcataccaac acctcaactt
       61 gcgcctgctc gtaatcatgg aacccttctg ccaaatcttg cacgtgccgt tccacccccc
      121 caatccacgg gtaatataac ttactcacct gaagaatctt catgcttttt gtaaccacca
      181 tgtacttgaa gttggagtgt cttgcgtaaa tcgcactaac cggaatcccc ctgctttcag
      241 gtatgcaagc aagcgcgcac gctgcaattc gccaccccca acatcgtggc actccatgcg
      301 aatttcctga atcttcgcca agtatgtcaa aggagtgttg agaagaattt cgaactccgc
      361 accttcgcaa tcaatcttaa ggacatcgca ttgttcaatg ttattctcgt cgaaaatttc
      421 ctgcagtgtt atgcatccaa tctcaacatg ccctttaccc tcttgggttc ggtaaaaaga
      481 gtgaaaagga gaaccatttg ctaaaaagag ttgccttcgt cccctcgagc ccgccacacc
      541 cacgcgaaaa ggaaaaatga acttctgcag tctgttccgt tgcatattct taagaagcag
      601 gctataactg cgcggcaacg gttcgtatgc atacactttt gtgttttttg acgtccctgc
      661 cgcataaacc gcatatacac caatattggc gccaatatcg ataatggttg tattgtcttt
      721 cgcgtctcca taagcttttt tcacgaaaac gacagctatt gttgcagcat cgacaccttc
      781 tccggtttcg atttcaatgc cgttccgcaa ccggtagacc gccccgtaat ttttaacccc
      841 aatgtagttt aaaagaaaag gccaccaatt cgcaatttgc tgaactaccg aaggaatgta
      901 tattgtatgc tttatagcgc caagagacgc aaattcgctt aagctccttc tcataatcat
      961 ttggtcaccc gaagctcaaa gtaaagttcg gctgcaggaa caatgaaagc aaagagtcgt
     1021 tagtagtgcc gcgggaagcg gtttgcaaga tattcaactc cgagaatttt ccatacattg
     1081 ggaaaatgct ttttccccga tggcaatact tcagaaaatc ctatatattt cttctcgacc
     1141 cgaaaccgtg ccttcgaata aaactgatgc acattgttct gaaatcgcgg gtcaaaaaaa
     1201 tcaaaactgc gcgaagaaaa cggatgcttg tgggtaatat cggtgtagta gttatcagaa
     1261 ctaaaatgcg gcaccgagat gataatttct cccccaggtt ttgtaatgcg atggagttct
     1321 tccactaccg ccacaatatc gtctaagtgt tccagcacat cgtcgcaaat aatttggtcg
     1381 aattcgttat ttccgaacgg gtagggaaaa acattcaggt cgtgcaccgc atctactcca
     1441 ggcatagcaa tcttgtcgat gccgataact tgcgccccct caatgacttt tttcctatcg
     1501 ccgcagccaa gatcaagtac cttcatagaa tatcaccata gtagtatagt caacgtgaag
     1561 tttcaacatg cttttgttct ggaacttcct tcttcatggt agcaaaatag atgtttgttg
     1621 acacgataac aaaaagaaat gccgcaatcg tagcaacaac agctccgtat aagcctatga
     1681 ctggtataag caaagtattg cagaatacat ttatcaccaa accagcccac ggaatgaaag
     1741 caccaaaacg tactgtcttg atctccatag aaaacaaaag ccagctataa atactactta
     1801 caaagaaaag aaaaccgttg actgaaaaca ggagaatgag taccacgtta aattcatatt
     1861 ctggaccgaa aagtaacaat gcaaaagcga taataccata taacaatccc gtggctggca
     1921 ccgcaagaaa agccataaat ctattaattt ttcgaaaaag aattttttta tggggagctg
     1981 cagagctata cgggaaaaaa actgcaagaa aaacaccgag ccccctttct agcataactg
     2041 tagaagacaa aacatacgcg ctgtagaaac ctacagctgt agagcctaag aaataatgga
     2101 tcatcagcct atcgatgtat ccaaaaagat accccggggt aaggccaaga aaaacccaga
     2161 tactaaaaga caagagggtc tttgtattct tccaatgaat acgcatgggg aatatcaaag
     2221 aacggaggag gaaaaatccg atgagtaccg ggaatagcca tcgcaggatt tctgcaaaga
     2281 taagcgaggt aaagcttttg tcttcgccca agttcgcaaa accaaaaaag aaaagcacaa
     2341 aaaaaagagc agacaaaaca tgaaaggcag caaatcttcc aaattgttgg agtccttgaa
     2401 aaagcgactt ggacacaaca acgaaaacat gggtaagtgc aaacgcaata gcaaacagaa
     2461 aaaccaggag atcgactcgc agtagtcgtg aaagggggtg agatacgagg agcaatacca
     2521 aggtcattgc aaaggcccaa gcaatagtta tcacaaaaag agatggaacg atacgcttaa
     2581 cttctctttc ctgaacaata ttctttacaa gggcgtaggg aaaaccgagc gtcatcacta
     2641 gaagaagaac gctgcctata gaaaacacaa gatttgcttt cccatactca aaggggccca
     2701 aaatccttcc ggccacgatc attacaaaaa aaccaaggac gctcgcccct aacgtaccta
     2761 ccgacacgaa agacacattt tgccaaaagc gctttgcttc agggcccatt ggatatccaa
     2821 acactctctg ccatatgata tctccaagtt tgaaatagtt cataaaaaaa ttcatttact
     2881 aggaaaagag gaaacgcctt tttggcactc gggctccagg agaaatcgaa ttatctccct
     2941 ctcgttttcc gaggaagttc ctctttctaa gtaattcttc atttgctttt gtacctcttt
     3001 aaaaggcgta gtttctctat tgacgcacaa aagaagcatc cacccttttt ggatatccgc
     3061 atttgaagaa accacacctt caagcagcaa aaacccctcc tggaattttc cgaggcggaa
     3121 ctttgccaag gccagccaat attggaactc ttgccgttcg ggcgatttga ggagcgcttc
     3181 tccccaaaca cgctctgctt tccccagcac ttcttgttct tggaaaagct cggcccccgc
     3241 aaagtaaata tgcccaaaca aaaggccgtg ccgcacttcc aagggctctt gcctttggag
     3301 ttcctgcaat tccttttctg caaatagaaa gagtgtggcc gcatcttttt tgttgccttg
     3361 ctcaaccaac gtatacagca caggcatagt ctggttgaga acgtaggctt ttgtgctcct
     3421 cgtgtaaggc gaaggaaatc tctctgctct ttcataccac gcaatggctt ggggcacgtt
     3481 gtctggaaga ttgtcttttg ctttcgcaaa aagccggtcg accccgacag gaacaagata
     3541 tcccgccaaa agagccgtgg tgacaagcac tattgcgccc gccaggaaaa cttttttgcc
     3601 aaggcgtttt ggtttttgcg cagcttcttt agcgagatgt atttggctgc ccataaacgc
     3661 cagcacaagg aacagcgaaa tataagaggt agggatgtcg aaaagaaaaa ggccgaatac
     3721 cagatgtgcc accaaaaacg cgccggccac gccaaccaca gccggctcaa tttgcttttg
     3781 ccgatagcct tgccataaaa gaaatgccgc cgcgccatat atgccaagca aagcaaacag
     3841 ccctgttatt ccttgcgtgg ccagggcttc caacagcaca ttatgggcaa cgtcaaacca
     3901 ggtttcgggc tctccatact gaagaatttc gggcggataa tacgcgtgaa acgcataagc
     3961 aaaattccca ggcccccacc caaacaaagg tttttctttc caagcttcaa aagctacatt
     4021 ccaagccaaa agccgcgtat ggagagttgc agtggctccc tggcccacaa aacgttctac
     4081 cgcaggcacg tctgccacag aaaacgcatt ctgaacaaca aacacaagac ttacccctcc
     4141 aaaaataaag aggggcagca gcataaaccc tatggtgcca aatatatgct ttctcccttc
     4201 ctttccggct cttttccaca atacagccaa agaaacacac aaaaacaaaa gcccgcacgc
     4261 caagcccaga aacgcccctc ttgtaccgct tgccaacact ccaaacaacg aaactacggc
     4321 gccggccaaa gcaaaactaa accaccgacc ttgtttttct tgcgccagca aaaagtaggc
     4381 aacgaacaca agcgccatgg caaagccgcc aaaaaaggcg gcatttccca atgtagaggc
     4441 cgctcgaagt acttcttccc ccttttggta caaagcctgc ggaaacccaa cctcaaaaat
     4501 gagcgcaaaa agtatggcaa gtattcctcc tccaaggaac acccgaagca cccagcgcca
     4561 gtcttctttt tgcaacacct ggcctaacaa aaaatagtac gcaatgcagt gcaaaagaaa
     4621 aacaacgccg gtaagtcgca gcgatgtact ccacaaactt cggtaccagt ctacgccaaa
     4681 aagcgtggaa acgagaaggc ttgcgcccca caaaagaatc gcccaggaaa gtatgttcaa
     4741 acgaatccgg tatttttgaa aatctaccag cgaaaggagc gcatatattc cggctaaagc
     4801 tacaacaata ggttggaaaa agaacacttt ggggccaagc gcaggaaccg tataggcaga
     4861 acctgccagc agcggcaaca aaaggcttac tccgacaagt attttaaaaa gaatttccaa
     4921 agcctgctgg acaagaaaca taaagtgccg agggtgggac tcgaacccac aagccttgcg
     4981 gcgctagctc ctaaggctag tgcgtttgcc aatttcgcca cctcggcatg cgtttgctgc
     5041 caacctgccc cgtgattcgc gaagcgagtt ttcggggttc cgccacgggc gcgcaaaacc
     5101 aatatgctta agtatgcctt aaaatgcccg aaaaataaag aagaagctcg gcgtaacaag
     5161 tacaccgagc cttacactac ttgaggctta acgcacattg gcgtatattg ctcggggtcg
     5221 aaagccccgt aagctttgcc tctagttcca gactactggc atctgtctcg aaagacgagc
     5281 cgacgtctgt aataaattcc ccttcctccc tggtgcgata tgccgcggta tagccaaacg
     5341 gacagaccag agaagaaaaa accgtgtagc tctggttatc gtctaccgcc tttatgagag
     5401 ttaccgttat ggtaccctct tctggccggt acacaggatt tccaaacaac gtatatggat
     5461 ttgcgacttc gaatcctgtt tcgttgtcgc agttcccgct caccaagacc tgctttccca
     5521 tccactcttc ctcacccgta gcctgccttc tacgatatac attgcgagat accgcgtcga
     5581 agcacttgtc atgtgcgtca aacgaagcaa agaactcgct ttcgaacgcc tctccaccgt
     5641 cgaatgcaaa gtacgtgcag tcgggaaaca tttggccacc ccttacgacc ctgtcgccca
     5701 aaccgtgtgt cttattcacc gccaccccgt tctcctcgaa ctggtggcac accttaacct
     5761 gttcatccgt atgcggtttt ggcggaaggt tccctgatac acgtttactg ttgttccggt
     5821 acagcatgat tccttgtgta cctggcgcta caggctgcgg cgtcggagtc gggataaggg
     5881 ttggcgtggg tatagccgta ggcgtcggca tcggagttgc gtcgggtgct acgccgggaa
     5941 ccggtgttgc caccggcgtt tcgataggcg catacttgcc gcgcaactcc tctgctacgt
     6001 gctttggccc tttaacctgt acgctgtcga tcgaaggccc aaataaaccg ggcgtgtcgc
     6061 tcacaaaacg cacgatagtc ctttcgccac gggcaataaa aggcaattct cgttctaccc
     6121 accccatatt gaaaaggctc ttaccgcgaa tgtcaaagga aaaaggctct accttctcag
     6181 aatctatttg aattatttcc acccttcccc ttttttccag tggcccgttg gcagtgttgc
     6241 cagcaagcct gtacgacacg ctaaaaggct ctcctggaat agtgggaaat tcccacgccc
     6301 acgcccctgg ccctgccacg ccattcagat ctatcgactt tgcacccaat gcggactggc
     6361 gcaaactaga gtcaaaatga tgtatagaac cccattccac cataccgccc gcaaacggcg
     6421 ctatagcctt gtagggctct ccatacccag ggctaggtat gtgctcgtcc tcaaaatcct
     6481 gctcccatat tttttcgaac cccacagtag agatcgactc gggcgatgga ggcggcacag
     6541 gggtggcggt tggagctggc tgtggggtgg gggtagcaac cgccgcatct gccgcaggca
     6601 cccacctccg agtcgtagtg gttgtggttg tgcaaccaaa tattcccaaa gcacagatta
     6661 ccaatagtag taagaacagc gtcttcatac tctccctcaa ctaaaaactg cattcggata
     6721 tgcagtatat attatcacaa atctgttgta tgtcaagctc tttggggtgc cggcgtttcc
     6781 gcgcgcgccc gcaaggccca acgcacaaca aacacagaaa gtgttgccac ggcgattccg
     6841 gct
//
LOCUS       DASRUP010000013        10343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1624314, whole genome shotgun sequence.
ACCESSION   DASRUP010000013 DASRUP010000000
VERSION     DASRUP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624314"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFE94_00635"
     CDS             <1..877
                     /locus_tag="VFE94_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZX49642.1"
                     /translation="GEKARDVKDTSLLMPHGKRGRVVNVKVFSRDEGHKLEPGVMKRI
                     HVEVAELRKVQRGDKLAGRHGNKGVISVVLPEADMPYMEDGTPVDIILNPLGVASRMN
                     IGQILETHLGWAAHELGYMAETPGLAGATEKDIEDELEKAGLPKDGKVQLFDGRDGTA
                     FPHKITVGYIYMMKLIHMVEDKIHMRSIGPYSLITQQPLGGKAQFGGQRFGEMEVWAL
                     EGYGAAHMLQEMITIKSDDVPGRAATYESILRGQPIQTPNLPASFNLLLNELRSLGLQ
                     IEIKGKQKEEKDESR"
     gene            864..4478
                     /gene="rpoC"
                     /locus_tag="VFE94_00640"
     CDS             864..4478
                     /gene="rpoC"
                     /locus_tag="VFE94_00640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZX49643.1"
                     /translation="MRVVDLEAIRIKLASPEDILSWSHGEVTKPETINYRTQRAEKDG
                     LFDERIFGPEKDYECYCGKYRKIRYKGVVCDRCGVEVTKASVRRERMGHIKLATPCSH
                     IWFLKGVPSRMGLLLDIPAQGLEKIIYFAAYVITSVNEEARQKALEEIETEFKQRTKG
                     KTGEDKEKLKAARDRARQEVHSLTPLRIVSEVEYQNLALKYGQAFEAGTGSEALRAIF
                     ENVDLKKLARDLFGQLDNVTPQNRKKILARLRLVHGMQQAGIRPEWMFLTMIPVLPPD
                     LRPMVQLDGGRYASSDLNDLYRRVINRNNRLKYLLEIGAPDVIVRNEKRMLQEAVDAL
                     IDNNMRKGTMTAATTGGKRLLKSLADMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPAL
                     KLHQCGLPKRMALELFKPFVIQKILEKEFAYNVRGASRLIEQETDEVWAILEEVAKGK
                     LVLLNRAPTLHRLGIQAFYPVLVEGEAIHLHPLVCEAFNADFDGDQMAVHVPLSTEAQ
                     EEAKKLMLSSVNLLKPATGNPIASPRQDMVLGCYFLTGIVKEQQVKKQRMLSSKDEAM
                     LAYEQGAIGLQEMVKVRIGAEMVETTVGRMIFNGSLPEDYAFVNEQMTAKKLGAIMGD
                     IVKRYPQQTSEEILDGIKELGFEYATKSGTTWGMTDLVVPLEKKELLAQAEKEIEAID
                     THWHHGLLSKEERSVKVIEVWQRVKSTIEQLVPKTLPEGGSVFAIIDSGARGSWSQPI
                     QMSGMKGLVINPAGRIIELPVKNSFKEGFDVLEYFISTHGARKGTADTALRTSTAGYL
                     TRRLIDVSHDVLIVEEDCKDNKGMVVLKKDAEDLGQAMPLKIGGRVAGEAVGDYVKKG
                     EIIDWKTANKIGEDPNITEIRVRSPLSCKTPRGLCQKCYGWDLGNSKIAEIGSAVGIV
                     AAQSIGEPGTQLTMRTFHTGGVASGGDITQGLPRVEEIFEGRVPGGKAVISEVEGIVE
                     EITQDGVIKIRPTGKDKIIEPKEPPKKKTVRRTQGKKKEQSGEFMEYEIPAKRALWVQ
                     KGSQVVKGQQLCEGNLDLAELFKNTGKEETQAYIAKQVQQIYTSQGASIHDKHIEVIT
                     RQMFSRVRIKTPGDSKFAEAEVLERGTFQEEKERLEKAGKTVPVAVDLLLGISKIALT
                     TESFLSAASFQETSRVLIQAALEGREDRLRGLKENVIIGKLIPAGTGFKPKEKPTAVK
                     "
     gene            4526..6739
                     /locus_tag="VFE94_00645"
     CDS             4526..6739
                     /locus_tag="VFE94_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZX49644.1"
                     /translation="MQEFELWGISLYNRDIVGKKKKKNGGGLFHLPLPSFSVNLPERA
                     QRMVLSIAFFAFALVFVFAFFGRAGAAGNMLFEGAQFLVGRGVFLLPLFLIGTGAVLW
                     GMKKYSTFLAFGALFLVVVSTSGILGTFARSRNLQVAELGGFIGSAVSLPVFAAFGVW
                     VGEIIFFSLVAVGAVILWQLLPHEKVEPSSFATGAQEKLKRIFEPKFQVSKVEPEPKV
                     QSSTIFEAEEQRLKEAEKEKRKEKKAEPTSAFLNYKPPTTDLLEPEKGVPNAGDVKTY
                     SAIIKKTLQNFDIPVEISEVNIGPAVTQYAFKPAEGIKLSRITALQNDLALALAAHPL
                     RIEAPIPGRALVGIEIPNKTRATVRLRSLVETQDFQDSTASLLLALGKDVSGAPIFAD
                     LARMPHLLVAGATGSGKTIGLTNVIISLLYRNPPSNLRLILVDPKRVEFPVYNDLPHL
                     LTPVIFDTQRTLNALKWLIKEMERRFSVFSDARVRDIKGYHAYYEEQIKKGKEDAEEI
                     PYIVTVIDELADLMAARGKELEGYIVRLAQMARATGIHLVLATQRPSVEVITGLIKAN
                     ITARAAFQVASQIDSRTILDSAGAEKLLGQGDMLFVSAEFTKPRRIQGAYVSEKEVKN
                     IVGWIAAESDTVSREEMDGNDLAARAKESMENGFDESDDDLLYEEAKRVVMESGKASA
                     SLLQRRLKVGYARAARLLDILEEKGVVGPGEGAKPREVYGSRAQGQDTQGEWINP"
     gene            6747..7748
                     /gene="recA"
                     /locus_tag="VFE94_00650"
     CDS             6747..7748
                     /gene="recA"
                     /locus_tag="VFE94_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZX49645.1"
                     /translation="MAKKKEPRKYAEDLNEALDEIKQRFGEGAIMKLSEARHVDVDVI
                     PTGSVSVDLALGVGGVPRGRIIEIYGPESAGKTTLALHVMSEAQKKGGVGAFIDAEHA
                     MDPDYAKRIGVNVNDLLISQPDSGEQALQIVETLVRSGEIDVLVIDSVAALTPKTEIA
                     GEMGEFQIGLQARLMSASLRKLSGLVAKTKTSVIFLNQIRMKVGVIYGNPETTPGGLA
                     LKFYSSVRIEVRRAAQIKHGEEIIGSRIRAKIVKNKVAAPFKTAEFDIYYNEGISYEA
                     DLLNAGIREGVVKKAGSWLQFEDAKLGQGMDASRAFLKENPAVGKKIRETLLSQSRK"
     gene            complement(7725..7922)
                     /gene="rpsR"
                     /locus_tag="VFE94_00655"
     CDS             complement(7725..7922)
                     /gene="rpsR"
                     /locus_tag="VFE94_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZX49646.1"
                     /translation="MECYFCKKNIKEVDFKETVMLRRFVSGLGKIRKRTRTGLCATHQ
                     RKISHAVKRARHMGLLSALAK"
     gene            complement(7924..8373)
                     /locus_tag="VFE94_00660"
     CDS             complement(7924..8373)
                     /locus_tag="VFE94_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZX49647.1"
                     /translation="MNFNKAIIIGNVVADPESRTTPSGQQVCSLRVATNRTWTDKAGA
                     KQQKAEFHNVVLWARLAEIASQYLKKGSLVLIEGRLQTRSWDDQTGNKRYRTEIIAEN
                     MQLGPRGFNQGGGEEQRPPAKEQEQQPKDEIPIIEEDEEIDVKDIPF"
     gene            complement(8370..8813)
                     /gene="rpsF"
                     /locus_tag="VFE94_00665"
     CDS             complement(8370..8813)
                     /gene="rpsF"
                     /locus_tag="VFE94_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZX49648.1"
                     /translation="MRNYQLTLIFSPILSEQEANDQMQQLASLVQEKGGILGEQQMLG
                     KRPLLAPIKSQKEGFLASLAFTVSPEYLGELEAQYKGNSRVLRSLLAQRVRKSAKAGR
                     VPQLKQQTETEGTQETQQQREPRKEEKIDLKDIDEKLEEIFKESK"
     gene            8871..>10343
                     /locus_tag="VFE94_00670"
     CDS             8871..>10343
                     /locus_tag="VFE94_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZX49649.1"
                     /translation="MHIPETIFREYDIRGIAGKEFSPQEIAKFEKWYGTFPGITLTVE
                     SAKAIGKAYGTLIRRQQGKKVVVGYELRPFARQLDEAFIAGVLTTGCDVLDAGVSLTP
                     FVYFAVAHFDLDGGVNITGSHNVYFYNGFKMMKKGVWPLFGKELQEMRELAEKEDFVQ
                     DKEGELGKLEVYEAYEKYFTEHIKLARPLKVVVGTGNGSAGMFAPQLFRKLGCQVVEL
                     YTEPDATFPNHDPDPEAPQNMKDLAEKVRQEKADFGIGLDADGDRAGFVDEKGEFVSA
                     DLALLLFAKDALSRNPGKKILYDVKSSKLLEELIPGYGGVPLMHRTGHAPIKETLRKD
                     PDIIFGGEVSGHFYFVEQYFKIDDGLFAAGKMLELASRHDGPFSSLFAEFPYRVRTPE
                     IKLGCSDEKKFEIVDQITQTLKAKYPVTLIDGIRLAISDTGWGLVRASNTSPYLTVRV
                     EGKTKEEVLQIKNILARELEKYPEVQDTLDTEHVASLSGKL"
BASE COUNT         2722 a         2321 c         2881 g         2419 t
ORIGIN      
        1 tggcgaaaag gcgcgcgacg taaaagatac ttctttgtta atgccccatg ggaaacgcgg
       61 gcgcgtcgta aacgtgaagg tgttttcgag agatgaaggc cataagttgg aacccggcgt
      121 aatgaagcgc attcacgtgg aggtggcaga gcttcgcaaa gtgcagcgcg gagacaagct
      181 ggccgggcgc cacggaaaca aaggagtaat ttcggtggta ttgcccgaag ccgacatgcc
      241 ttatatggaa gacggcactc ccgttgacat tatcttaaac cccctgggcg tggcttcccg
      301 tatgaacatc gggcagattt tggagactca cttgggctgg gctgcccacg agttggggta
      361 tatggcagaa actccgggtt tggcgggagc taccgaaaaa gacattgaag acgagctgga
      421 aaaagcggga ctgcccaaag acggcaaagt gcagcttttc gacggaagag acggaaccgc
      481 atttccccac aaaattaccg tgggttacat ctacatgatg aaattgattc acatggtgga
      541 agacaagatc cacatgcgtt ccattgggcc atactccttg attacccagc agcctttggg
      601 ggggaaagcg cagtttggag gccagcggtt cggggaaatg gaagtgtggg cattggaagg
      661 atatggcgcg gcccacatgt tgcaagaaat gattacgatt aagtcagacg acgtgccggg
      721 aagagccgct acctacgaat cgatcctgcg ggggcagccc attcaaacgc ctaatttgcc
      781 cgcatccttt aacctgctgt taaacgaatt gcgttcgtta ggcttgcaaa ttgaaataaa
      841 aggaaaacaa aaagaagaaa aagatgagag tcgttgattt agaagccatt cgcataaagt
      901 tagcttcccc cgaggacatt ttatcgtggt ctcacgggga ggtaacgaag cccgaaacca
      961 ttaactaccg cacccagcgt gcggaaaaag atggcctttt tgacgagcgc atttttggcc
     1021 ccgaaaaaga ttacgagtgc tactgcggga aataccgcaa aatccgctat aaaggcgtgg
     1081 tgtgcgaccg ttgcggagta gaggtaacaa aggcctcggt caggcgagag cgcatggggc
     1141 atattaaact tgccactccc tgctctcaca tttggttttt gaaaggcgtg ccttcgcgca
     1201 tgggtttgct tttagatatt ccggcccagg ggttagagaa aattatatat tttgcggcgt
     1261 atgttattac cagtgtgaac gaagaggcgc ggcaaaaagc gcttgaagaa atcgaaacag
     1321 agtttaagca gcgcaccaaa gggaaaacag gagaagacaa agaaaagctc aaagcggccc
     1381 gcgatcgcgc caggcaggag gtacattcgt taactccttt gcgcattgta agcgaagtgg
     1441 agtaccagaa tcttgcctta aagtacgggc aggcgtttga agccggcaca gggtctgaag
     1501 ccctgcgggc gatttttgaa aacgtggact taaaaaagct cgcgcgcgat ttatttgggc
     1561 agttggacaa cgtaactccc cagaatcgaa aaaagattct ggctcgcttg cgtttagtcc
     1621 atggaatgca gcaggcgggc attcgccccg aatggatgtt tcttacgatg attccggtgc
     1681 tgccccccga cctgcgcccc atggtgcagt tagacggagg ccggtacgct tcttcggact
     1741 taaacgattt gtaccgaagg gtgattaata ggaacaatcg tttgaaatat ctgttggaaa
     1801 tcggggctcc cgacgttatc gtgcgcaacg aaaaacgcat gttgcaggaa gctgtcgatg
     1861 ccttaatcga caacaacatg agaaaaggca ccatgacggc cgccaccacc ggaggcaaaa
     1921 ggctgttaaa gtcgctggcc gacatgctca aaggaaagca aggacgtttc cgccagaacc
     1981 ttttgggaaa acgcgtggac tattccgggc gttccgtaat cgtagtgggg cctgctttga
     2041 agcttcacca gtgcggtttg cccaagcgca tggccttgga attgtttaag ccttttgtta
     2101 tccaaaaaat cctggaaaaa gaatttgcct acaacgtaag aggagcgtcg cgccttatcg
     2161 aacaggaaac cgatgaggta tgggcaattt tggaagaagt ggcaaaaggc aagctcgtgc
     2221 ttttaaaccg cgctcccacg cttcaccgtt tgggcattca ggcgttttat ccggttttag
     2281 tggagggcga ggcaatccac ttgcatccct tggtgtgcga ggcttttaac gcggactttg
     2341 acggcgacca aatggcagtg cacgtgccgc tttctacaga agcgcaggaa gaagcaaaga
     2401 agcttatgct ttcttcggta aacctgttaa agcctgccac gggaaatcct attgcgagcc
     2461 ccaggcaaga catggtactg ggctgttatt tccttacggg cattgtaaaa gagcagcagg
     2521 taaagaagca gagaatgttg agctcgaaag atgaggcaat gttagcgtat gagcaaggag
     2581 ccattggact tcaggaaatg gtgaaggtgc gcattggcgc agaaatggta gagacaaccg
     2641 tggggcgcat gatattcaac ggttccctgc ccgaggatta cgcgttcgta aacgaacaaa
     2701 tgactgccaa aaagctgggg gctattatgg gcgacattgt aaagcgatat ccccagcaaa
     2761 cgtccgaaga gatcttagac ggaatcaaag agcttggatt cgagtacgca acgaaatccg
     2821 gcaccacatg gggaatgacg gatttggtgg tgcctttaga gaaaaaagaa ctgctggcac
     2881 aggcggagaa agaaattgag gccattgata ctcactggca ccatgggttg ctttcgaaag
     2941 aagagcgatc ggtaaaagtt attgaagttt ggcagcgcgt aaagtccacc atcgagcagt
     3001 tggttcccaa aacgctgccc gaagggggtt cggtgtttgc tattattgat tccggggcaa
     3061 gaggttcgtg gtcccaaccc attcaaatgt cgggcatgaa agggctggtg attaatccgg
     3121 cagggcgcat tattgagttg cctgtaaaga attcttttaa agaaggattc gacgtgctgg
     3181 aatactttat ttctacacat ggagcaagaa agggtactgc cgacaccgcc ttgcgtactt
     3241 ctacggcagg atatcttacg cgccggctta tcgacgtttc ccacgacgtg ctcattgtgg
     3301 aagaggattg taaggacaat aaaggaatgg ttgtactcaa gaaagacgca gaagacctcg
     3361 gccaggctat gcctttgaaa atcggaggcc gcgtggctgg ggaggctgtg ggggattacg
     3421 taaagaaagg agaaataatc gactggaaga ctgccaataa aataggggaa gacccaaaca
     3481 ttaccgaaat ccgggttcgt tctcctcttt cctgcaaaac tccgcgcgga ctgtgccaaa
     3541 agtgctatgg gtgggatttg ggcaacagca agatagccga aatcggatct gccgtgggta
     3601 ttgtggctgc ccagtctatc ggggagccgg gaacccagct taccatgcgt acgttccata
     3661 ccggaggcgt ggcttccggc ggcgacatta cccagggttt gcctcgcgtg gaagaaattt
     3721 tcgaaggacg cgtgcccgga ggaaaggcgg ttatctctga agtcgaggga atcgtggaag
     3781 agattactca agacggcgta ataaaaattc gccccaccgg gaaagataag attatagaac
     3841 ccaaagaacc tcccaagaaa aagaccgttc gacgaacgca gggcaagaaa aaagagcagt
     3901 ctggtgaatt tatggaatat gaaattcctg ccaagcgcgc cttgtgggtg caaaagggca
     3961 gccaggtggt gaaaggccag cagttgtgcg aaggaaacct tgatctcgcc gagttgttta
     4021 agaataccgg caaagaagaa acgcaggcat atatcgcaaa gcaagtgcag cagatttata
     4081 cttcccaggg agcttccatt cacgacaagc acatcgaggt tattacccgc cagatgtttt
     4141 cgagagtgcg cataaaaacg ccgggcgaca gcaaatttgc agaagccgaa gtgttggagc
     4201 gcggcacgtt ccaggaagaa aaagagcgtt tggaaaaagc gggcaaaact gttcccgttg
     4261 ccgtagacct actgcttggg atttccaaaa tcgccttaac caccgaaagc ttcctttcgg
     4321 cggcttcttt ccaggaaact tcccgcgtgt taattcaggc ggctctcgaa ggcagggaag
     4381 accggctccg tgggctcaag gaaaacgtca taatcggcaa actcattccc gccggcaccg
     4441 gctttaaacc aaaagaaaaa cctaccgcag tgaaataaaa aagcaagcgt aaaaaccgcc
     4501 tcggaggcgg tttttggtgg aggttttgca ggaatttgaa ctgtggggaa taagcctgta
     4561 taatagggat attgtgggca agaaaaagaa gaaaaacgga ggaggattgt ttcaccttcc
     4621 cctgccaagc ttttcggtaa accttcccga gcgcgcccag cgcatggttt tgagtattgc
     4681 atttttcgcc tttgccctgg tgtttgtgtt tgcttttttt ggaagggcgg gagcggcggg
     4741 caatatgctt tttgaggggg cgcagtttct tgtggggcgg ggcgtattcc tcctgccttt
     4801 gtttcttatc ggaacgggag cggtgttgtg gggtatgaag aaatacagca cttttcttgc
     4861 ttttggcgct ctctttttgg tagtggtgag cacgagcggc atattgggaa catttgcgcg
     4921 aagccggaat ttgcaggtag ctgaactcgg cggcttcatt gggtctgccg tgtcgctgcc
     4981 ggtgtttgcg gcattcggcg tttgggttgg cgaaatcatt ttcttttctt tggtggcggt
     5041 gggggcggta atactatggc aattgctccc tcatgagaaa gtagaacctt cttcgtttgc
     5101 tacaggcgcc caggagaagc tcaaaaggat tttcgagccc aaattccagg tttcgaaagt
     5161 agagcccgag cccaaggtgc agtcgtccac gatctttgag gcagaggagc aaaggctaaa
     5221 agaagcagaa aaagaaaaaa gaaaagaaaa aaaagcagaa ccaacttccg cctttttaaa
     5281 ctataagcct cccactaccg atcttttaga gccggaaaaa ggagtgccca acgccggaga
     5341 tgtaaaaaca tattctgcga ttattaagaa aaccctgcag aactttgata ttcccgtgga
     5401 aatttcggaa gtgaacattg gccccgcggt aacccaatac gcgtttaaac ccgcagaagg
     5461 gattaagctt tcgcgcatta cggccctgca aaacgatttg gctttggccc tggctgctca
     5521 ccctttgcgt atcgaggcgc ccataccagg tcgcgccctt gtcggaattg aaattcccaa
     5581 taaaacaaga gcaacggtac gcctccggag tttggtggag acgcaggatt ttcaagattc
     5641 tacagcttcc ctgcttctgg ccctgggtaa agacgtttcc ggagctccta tttttgctga
     5701 tctcgcgcgc atgccgcatt tgctggtggc aggcgccacc ggttcaggaa agaccatagg
     5761 cctaaccaat gttattatca gtttgttata taggaatccg ccttcgaacc tgcgcctcat
     5821 attggtggat cccaagcgcg tggagtttcc cgtgtacaac gatttgccgc atttgttaac
     5881 gcctgttatt ttcgacacgc agaggactct caacgcctta aaatggctca taaaggaaat
     5941 ggaacggagg ttctcggtgt tttcagacgc cagggttcgc gacattaaag gataccatgc
     6001 gtattacgaa gaacaaataa aaaagggcaa agaggacgcg gaagaaattc cgtatattgt
     6061 aacggtaatt gacgagcttg ccgatttaat ggctgcccgc gggaaagagc tggaaggata
     6121 tattgtgcgt ttggcccaaa tggcgagagc aacggggatt catttggtgc tggcaacgca
     6181 gaggccttcc gtagaggtta ttaccggttt aattaaggcg aacattacgg cgcgtgctgc
     6241 ctttcaggtt gcctcgcaaa tcgactctcg cacgattttg gattctgcgg gagcagagaa
     6301 gctgttgggg caaggagata tgttgttcgt ttccgcggaa tttaccaaac ccaggcgtat
     6361 tcagggcgca tatgtttcag aaaaagaggt aaagaacata gtgggttgga ttgccgcaga
     6421 gtcagacacg gtaagcagag aagaaatgga cggaaacgat ttggcagctc gcgccaaaga
     6481 gagtatggaa aacggttttg acgaatcgga cgacgacctt ttgtacgaag aggcaaagcg
     6541 ggtggtcatg gaatcgggga aagcctcggc ttctctactg cagcggcgcc tcaaggtggg
     6601 atatgccaga gcggcaagat tgctcgatat tttggaggaa aaaggagtgg taggccccgg
     6661 agagggagcg aaaccccggg aggtatacgg aagccgcgca caaggtcaag acacccaagg
     6721 agaatggata aacccttaaa aaacatatgg caaagaaaaa ggaaccaaga aaatacgcgg
     6781 aggatttgaa cgaagcctta gacgaaatta aacagcgctt tggagaggga gcgattatga
     6841 agctttcgga agcaaggcac gttgatgtgg acgtaatccc caccggctct gtttctgttg
     6901 atttggcgct gggggtaggg ggagttcccc ggggaagaat tattgaaatt tacggcccgg
     6961 aatccgccgg gaaaactacg ctggctctgc acgttatgtc ggaagcccag aaaaaggggg
     7021 gagtgggcgc ctttatcgat gctgaacacg ccatggaccc cgattacgcc aagcgcatcg
     7081 gcgtgaacgt aaacgatttg ctgatctccc agccggactc cggagaacag gctttgcaaa
     7141 tcgtggaaac gctggtgcgt tctggggaaa tagacgtgtt ggtaatagac tcggtggcgg
     7201 ccttaacccc caaaaccgag atagccggcg aaatgggcga gtttcaaatc ggcctgcagg
     7261 cgcggttaat gtcagcttct ttgcgcaagc tttccgggct ggtggcaaaa acaaaaacgt
     7321 cggtgatctt tttaaaccaa atccgcatga aagtaggcgt gatctacggc aacccggaaa
     7381 ccacgccagg cgggcttgct ctcaagtttt actcttctgt gcgcatcgaa gtaagaaggg
     7441 cggcgcaaat taagcacgga gaagagatta taggttcccg gattcgggcg aaaatcgtga
     7501 aaaacaaagt ggcagctccg tttaagaccg ccgagtttga tatttattac aacgaaggaa
     7561 tttcttatga agcagacctg ctcaacgcgg gaatacggga aggagtggta aagaaggccg
     7621 gaagctggct ccagtttgag gacgcaaaat tagggcaggg catggacgcc tcgagagcgt
     7681 ttctgaaaga gaatcccgca gtgggaaaga aaattcggga aactttactt agccaaagca
     7741 gaaagtaagc ccatgtgcct tgcgcgcttt accgcgtgag agatttttcg ctggtgcgtg
     7801 gcgcacaaac ccgttctcgt gcgttttctg atttttccca ggccggatac aaaacggcga
     7861 agcattaccg tttctttaaa atccacctct ttgatattct tcttgcaaaa atagcactcc
     7921 atactagaat ggtatgtctt taacatcaat ttcttcgtct tcttcgatta taggaatttc
     7981 gtctttgggc tgctgttcct gttctttggc cggaggcctt tgttcttcgc ctcctccctg
     8041 gttaaatcct cgtggtccta attgcatatt ttctgcgatt atttcggtgc ggtagcgctt
     8101 gttgcccgtt tgatcatccc aactgcgtgt ttggagcctg ccttcgatta acaccagact
     8161 tcctttttta aggtattggg aggcaatttc tgccaatctt gcccagagca ccacgttgtg
     8221 gaactctgct ttttgctgtt tggctcccgc cttgtctgtc cacgtgcggt tggtagcaac
     8281 gcgaagcgag catacttgct ggcccgacgg cgtggttcgg gattcgggat ctgcaactac
     8341 gttgccgata ataatggctt tattgaaatt catttcgact ctttaaagat ttcctccagt
     8401 ttctcgtcga tgtctttcaa atcgattttt tcttctttgc gcggttccct ctgttgttgc
     8461 gtttcctgcg taccttctgt ctctgtttgc tgttttaact ggggcactct tccggctttt
     8521 gccgattttc tgacgcgctg ggccaaaaga gaccgaagaa cccggctgtt gcctttgtat
     8581 tgcgcctcga gttctcctaa gtattcggga gaaaccgtga aggcgaggct tgccagaaac
     8641 ccttcttttt ggcttttaat gggagctaaa agagggcgtt tccccagcat ttgctgttcg
     8701 cccaggattc ctcctttttc ctgcaccaga gaagctagtt gctgcatctg gtcgtttgcc
     8761 tcctgttctg acaaaatcgg ggagaaaatg agggtaagtt ggtagttcct catggtatgc
     8821 acagtgtagc atattgccag aaatggtcaa gccatggtat ggagtaaact atgcatatcc
     8881 cagagacgat atttcgcgag tacgacatcc ggggaatagc cgggaaagag ttttcgcccc
     8941 aggaaatcgc caagttcgag aagtggtatg gcacatttcc cggcattacc ctgactgtcg
     9001 agtctgccaa agcaattgga aaggcgtacg gaacccttat tcggaggcag cagggaaaga
     9061 aagtggtggt aggatacgaa ctgcggccgt ttgccaggca actcgacgaa gcatttatag
     9121 caggggtttt gaccaccggg tgcgacgttt tggacgccgg cgtgtcgctt accccttttg
     9181 tctattttgc cgtggcgcat tttgacctgg acgggggcgt gaacattaca ggcagccaca
     9241 acgtatattt ttacaatggt tttaaaatga tgaaaaaagg ggtctggcct ttgttcggaa
     9301 aggaattgca ggaaatgcga gagctcgcag aaaaagaaga ctttgtgcag gacaaagaag
     9361 gggagctggg aaaactggaa gtgtatgagg cctatgaaaa gtacttcaca gaacatataa
     9421 agttggcgcg gcctctcaaa gtcgtggtgg gcacgggaaa cgggtctgct ggcatgtttg
     9481 ccccgcaact gtttcgcaag cttggatgcc aagtcgtaga gctttacacc gagccagacg
     9541 ccacttttcc caatcacgat ccagatcccg aagctccgca aaacatgaaa gatttggcgg
     9601 aaaaggtgcg gcaggaaaaa gcggattttg gcataggctt ggatgcagac ggggaccgcg
     9661 ccgggtttgt ggatgaaaag ggagaattcg tttcagccga tttggcactg ttgcttttcg
     9721 cgaaagacgc gctttcacgg aatcccggca aaaaaattct ctacgacgta aagtcctcaa
     9781 aacttttgga agagcttatt cccgggtacg gcggtgttcc tcttatgcat cgtaccggcc
     9841 acgcccccat aaaagaaacg cttcgcaaag accccgacat tatttttggg ggagaagtat
     9901 cgggacattt ctatttcgta gaacagtatt ttaaaattga cgacgggctt tttgcggcag
     9961 gaaaaatgct ggagttagcc tcccgccacg atggtccttt ttcttctctg tttgcggaat
    10021 ttccctacag agtgcgcacg cccgagatta aactcgggtg ttccgacgaa aaaaaatttg
    10081 agattgtgga tcagattacc caaacgctta aggcaaagta ccccgtcacg ctcatcgacg
    10141 gcatccgcct ggcaataagc gatacgggct ggggtctggt gcgggcttcc aacacttccc
    10201 cctaccttac ggtgcgtgta gaaggaaaaa caaaagaaga ggtcttacaa atcaaaaaca
    10261 ttcttgcccg tgagcttgaa aaatatcccg aggtacaaga cacgctcgat acagagcatg
    10321 tagcaagctt gagtggaaaa ctc
//
LOCUS       DASRUP010000014        11872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1183599, whole genome shotgun sequence.
ACCESSION   DASRUP010000014 DASRUP010000000
VERSION     DASRUP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183599"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFE94_00675"
     CDS             complement(<1..188)
                     /locus_tag="VFE94_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZX49650.1"
                     /translation="MSGHSHWAGIKHKKGLADAKRSQVFSRLAKELTVAAKEGGADQE
                     TNPRLRTVTEKAREANMPS"
     gene            complement(200..1174)
                     /gene="ruvB"
                     /locus_tag="VFE94_00680"
     CDS             complement(200..1174)
                     /gene="ruvB"
                     /locus_tag="VFE94_00680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZX49651.1"
                     /translation="MAENFINNLRPRLWGEYIGQEKIKNSLEVIIQAAKKRSDPLEHL
                     LFYGNPGLGKTTLAHVVAAEMQASLKTCSGPTLEKAGDLASLLTNLEDGDILFLDECH
                     RVQKAVMEMLYSALEDFQLHLVMGKGPMARTMDLDLPRFTLIGATTRVALLPAPFRNR
                     FGAIFQLQFYTLHDMEKIVQRSSSILEIPIAPAALSLIASRSRYTPRIANRILKRVRD
                     FATIEGSGIVTEQNAKKALEFLEIDELGLEPADRKLLETIIKQFGGGPAGIQALAAAN
                     AEEEDTILDLYEPYLLQLGLLERTPRGRMATPFAYRHLRIPTKQPELL"
     gene            complement(1368..2696)
                     /locus_tag="VFE94_00685"
     CDS             complement(1368..2696)
                     /locus_tag="VFE94_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZX49652.1"
                     /translation="MPSSHNTPPSENPPEAREELTGLIHINPSLLEETRQSVRNLEQL
                     REKLQQLQQPTGITPFFHTLLVAAISLDTSDIHVEPEEGKAQLRLRIDGMLHDLMEIP
                     FPLYENLLSRIKLVSEVKLNVADKPQDGRFSFELTQGELIEVRTATLPAEYGESLVLR
                     VLNPRSLITIEELGLKGSMLQLLKQELFKPNGMIIATGPTGSGKTTTLYAFLKEIVKP
                     EIKIVTIEDPIEYHLEGISQTQVNPEKGYDFASGLEAIVRQDPDIILVGEIRDKETAQ
                     IAIQAALTGHLVLSTLHTNDAPGTVSRLISLGAQAVNIGTASNVILGQRLVRKVCPHC
                     SVLTAPTEQELEKIKKALPSHDISSLQISKVKGCAQCNNAGYKGRIGIFEVILIDPAM
                     EEFILTNPSTAALRKFAITQGMVTMHQDGILKVLEGITTIDEVERVTGNI"
     gene            complement(2680..3495)
                     /locus_tag="VFE94_00690"
     CDS             complement(2680..3495)
                     /locus_tag="VFE94_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49653.1"
                     /translation="MEPTALLSHSKNILILLSKEATLGDTHLSLVLLAGLKKLGKTAT
                     VQQGNGLRENLFSPSVDHKKTFVVSLKGISPWISKIQYEKSEKDVKLYFTLQDGEVSP
                     EALALQVQNQTDLTIIVGEKSQKNNLFPYAGASFLWAEEARDALFELLTTHENGEHKM
                     FGHILGKLQFAERLDLYLTSLAPEDFAESRVEQKTLVALLEELQQTFSEQASYLFLFQ
                     AFPSTLPQGLAWTKNPAVKKLLHGAGQTQEKGNWILLSLPNTSIHQLTYALLS"
     gene            3532..3972
                     /gene="tsaE"
                     /locus_tag="VFE94_00695"
     CDS             3532..3972
                     /gene="tsaE"
                     /locus_tag="VFE94_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZX49654.1"
                     /translation="MSFFSKDPIYTHSTLETKALGALVAKSFLQGTKETNRAVVLALE
                     GELGSGKTTFVQGFAKALGIQENVLSPTFVIVREHGKLYHIDCYRLSSGEELKALGWD
                     KMVSHPGSFVLVEWPENIRELLPPYTKTISFATLEGEERVISFR"
     gene            3976..6222
                     /locus_tag="VFE94_00700"
     CDS             3976..6222
                     /locus_tag="VFE94_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HZX49655.1"
                     /translation="MKPKTLLLIDANSLLHRAFHALPPLSTPKGEMVHAVYGFLSIMF
                     KAIKEFQPSYIAAAFDVAAPTKRHKKFTQYKATRVKAPDELYSQFPLAKEALAAFHIP
                     VYEKEGYEADDIIGTLAGKASGMRSEQPIETIIVSGDLDTLQLVDKNTKVYTMRKGLQ
                     ETALWGQEAIAERFGGLKPAQLLDYKGLRGDPSDNIPGVAGVGEKTALQLIGEFGSLE
                     ALYNALEKKTSGAQTIKPKLKEKLLSEKEQAFLSKELVTIEKEAPVDFHLEDLAWEDY
                     DQEAAAELLLGWDFRSLVQRMPGERREGLKEGRGSQRDMFRESVESKIEKLHEEQVLS
                     NEVYELEKRLTPVLRNMEEKGLKIDKPYFAKLGQEMQKEIEVYAQRIHEMARKNFNVN
                     STQQLSEVLFGLPPDGLGISLKGLKKTPKGVVSTASPELEKLQEDHPVIREVLSYREL
                     QKLYTTYVKPLPLLADSEGRIHTHFDQLGAATGRVSSSEPNLQNIPNQGDWGRRIRRG
                     FIAKEGYKLVSFDYSQMELRIAAHIAGDKRMQQFFREGADIHRMTASTVFGMAPGEVT
                     DQMRFRAKALNFGVLYGMGVQGFAKSAGIPTQEARDFIENYFVRFPQLHEYMEHMKEF
                     AREHGYVETIMGRRRYIPEIHSIAPQLRSAAERMAINHPIQGSLADIVKMAMVRIEEQ
                     LPDPQGKRSMLLQVHDELLFEIADGIMEEIIGPIRRIMEQAGKLDVPVRVDVKKGKNW
                     NDVEKVLA"
     gene            6253..6642
                     /locus_tag="VFE94_00705"
     CDS             6253..6642
                     /locus_tag="VFE94_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX49656.1"
                     /translation="MKKVLVIEDEALIADLLSRRLQEKGYYAFLAEDGEKGLQFIRKE
                     APDLVLLDIVLPRLNGFEVLEELQKEGLLSRIPVIIISNSGQPAEIERAKEAGVVDWL
                     IKTEFDPQEVIEKVEKQIGPGDLAMQT"
     gene            6652..7023
                     /locus_tag="VFE94_00710"
     CDS             6652..7023
                     /locus_tag="VFE94_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX49657.1"
                     /translation="MSKILLIEDDKFLRELIIQKLGKEGYEVEEAPEGEGGLTKMKSG
                     KPDLVLLDLILPGIDGFEVLSKMKEDPELQGIPVIILSNLGQRDDVQKGIDLGAKDYL
                     IKAHFTPGEIVEKIKATLSAK"
     gene            7020..7973
                     /gene="holA"
                     /locus_tag="VFE94_00715"
     CDS             7020..7973
                     /gene="holA"
                     /locus_tag="VFE94_00715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZX49658.1"
                     /translation="MILFLYGQDTYRSRQKAAEIVKEYQKVRPRAVHVRAIDCSKADA
                     EDLKNEFDTISLFEQKKLLLLRDLFSNAEVVRFFDERKKQLLESSHHIVVVLQQAELK
                     DKTKNPLYQWLKKHATCQEFSPLPPAKLLLWIQREFTRYGVACSSRAAELLALRAGND
                     LWTLSQEVRKIAAFKKSEAKPAVKESDISLLVEGKAQADVFATIDALASRNKKQAFDL
                     LLRHLQKGDSAYYLFSMMHYQFRVLLEIRDMLDRQLSFAQMVSESKLHPYVLKKGVGV
                     AQNFSSPQLKNIYQKLFEVDFAMKTGKVESEGAFDVFVASL"
     gene            complement(7963..8238)
                     /gene="rpsT"
                     /locus_tag="VFE94_00720"
     CDS             complement(7963..8238)
                     /gene="rpsT"
                     /locus_tag="VFE94_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZX49659.1"
                     /translation="MPITKAARKALRQNPKRKARNLKKKNKVKTLTKQVRKLALAKTQ
                     QEAQQLLSQVYKALDKAAKTGVLKKNAASRKKSRITKFLLKQDSPKG"
     gene            complement(8270..10189)
                     /gene="uvrB"
                     /locus_tag="VFE94_00725"
                     /pseudo
     CDS             complement(8270..10189)
                     /gene="uvrB"
                     /locus_tag="VFE94_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
     gene            10199..10768
                     /gene="ruvA"
                     /locus_tag="VFE94_00730"
     CDS             10199..10768
                     /gene="ruvA"
                     /locus_tag="VFE94_00730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZX49660.1"
                     /translation="MISYLKGNILRKDSDYIILQTAGVGYKVHVPEVVFMQCKEGQQD
                     FEVFCALQIRKDETLELYGVPTFEALKLFEAFRDVSGIGPKAAMVLSSLGKPEDIKKA
                     IDQRNTLYFKGIKGIGEKKVQKLILELTGKLGSFESGKKSFGDPIVDSLVNLGFSKAE
                     AKEALLQLPADVTDPEEKIKAALKLLGRR"
     gene            11164..11661
                     /locus_tag="VFE94_00735"
     CDS             11164..11661
                     /locus_tag="VFE94_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49661.1"
                     /translation="MKLPRGPTLLRLLIMALALLLVLPAVASAKESSADAAGSTITLR
                     FSEATTSEGGTTTVKVAVVEEEGWVVSEQTLDLGRSGHQLTLLVDGTSVVVSTHVRGV
                     NPLRSNELAAKMLTIGLLTTQGPELSPIGISAESRATFEGIEVQGGSVAMSAQLVGTC
                     VPCHR"
BASE COUNT         3023 a         2591 c         3153 g         3105 t
ORIGIN      
        1 gaaggcatat tggcttcgcg cgccttttcg gttacggtgc gcagcctcgg gttcgtttcc
       61 tggtctgctc caccctcttt tgcggccacc gtgagctctt ttgccaaacg ggaaaagacc
      121 tgactccgct ttgcgtcggc caagcccttt ttatgtttta tgcccgccca atgggaatgt
      181 cccgacataa tatagaaaat taaagcaatt ctggttgttt ggtaggtatt cgtaagtgtc
      241 gataagcaaa cggggttgcc atgcgccccc tgggggtgcg ctccaaaagt ccaagctgca
      301 gaaggtaggg ttcgtagagg tctaaaatag tgtcttcctc ttctgcgttt gccgcagcta
      361 aagcctgaat tcctgcgggg ccgccgccga actgtttgat aattgtttcc agcagttttc
      421 tgtctgcggg ctctaacccc agctcgtcaa tttcgaggaa ctcgagcgcc ttttttgcgt
      481 tttgttcggt aacaataccg gaaccctcga tggttgcaaa gtctctcact ctttttaaaa
      541 tcctgtttgc aattcgggga gtatagcggg atcttgaggc gataagacta agcgcggcag
      601 gcgcaatggg tatttccaag atagaagagg agcgctggac aatcttctcc atatcatgca
      661 gggtgtaaaa ctgcaactgg aagattgccc caaaacggtt tcggaagggg gcgggcaaaa
      721 gagcaaccct ggtggtagcg ccaataaggg taaagcgggg aaggtccaaa tccatggttc
      781 gcgccatggg tccttttccc attacgaggt gcagctgaaa gtcttcgagt gcagagtaga
      841 gcatttccat taccgctttt tgcacgcggt ggcactcgtc taaaaaaaga atatccccgt
      901 cttcaaggtt ggtaaggaga gaagccaagt ccccggcttt ttcaagagta gggcctgagc
      961 aggttttcaa agaggcctgc atttctgcgg caaccacatg ggccaatgtg gttttcccca
     1021 agcccgggtt tccgtagaaa aggagatgtt ctaaggggtc gctccgcttt tttgcagctt
     1081 gaataataac ctcgaggctg ttttttattt tttcttgtcc tatatactcg ccccacaagc
     1141 gggggcgaag gttgtttatg aaattctctg ccatagaata gggcccttga caaatatttt
     1201 aggatggtct ataatcatgg acaataggac atgtcctatt tcctaacccc ccaaagctgg
     1261 ggacctggaa ttattttatg acgggtagct cgggtaatgc tgagctattc ctctggatat
     1321 ttccaaattt gcgttccgaa caccaaatgg tccccggctt tagggggtta tatatttccc
     1381 gttactcgct cgacttcatc aatcgtggtg ataccctcga gcaccttgag tattccgtcc
     1441 tggtgcatgg taaccatacc ttgcgtaatg gcgaatttcc gaagagcggc ggtagaagga
     1501 ttggtaagaa taaactcttc cattgcaggg tctatcagga taacttcgaa gattccaata
     1561 cgccctttgt agccggcatt attacactgc gcgcatcctt tgactttgga gatttggaga
     1621 gatgaaatgt cgtgggaagg gagggctttt ttgatttttt ctaactcttg ctctgtgggg
     1681 gcggttaaaa ccgagcaatg cggacagact tttctcacta ggcgctgtcc tagtatgacg
     1741 ttggaggctg tcccaatgtt taccgcctgc gcgcccaaac ttatgaggcg ggaaacggtg
     1801 cctggcgcgt cgttcgtgtg aagtgtggag agtacgaggt gtccggttaa ggcagcttga
     1861 atcgcaattt gagccgtttc tttatccctg atttctccca ccaaaatgat atccgggtct
     1921 tggcgtacga tagcttccag gccggaagca aaatcgtatc ctttttccgg gtttacctgt
     1981 gtttgggaaa ttccttcgag atgatattcg atagggtctt cgatggtgac gattttgatt
     2041 tccggcttta cgatttcctt gagaaaagcg tataaggtgg tcgttttccc agaacccgta
     2101 ggccctgttg caataatcat tccattgggc ttaaagagtt cttgtttaag cagttgcagc
     2161 atggatcctt tcaggccgag ctcctcaatg gtaataaggc ttcgcgggtt gagtacgcga
     2221 agcaccagcg actcaccata ctctgcagga agggttgcag tacggacttc aataagttct
     2281 ccttgggtca gctcaaaaga gaaccggccg tcttgaggct tgtctgccac attgagctta
     2341 acttccgata ccaatttgat acgagaaaga aggttttcgt agagaggaaa agggatttcc
     2401 atgaggtcgt gtagcatgcc gtcaatacga agacggagtt gcgcctttcc ttcctcgggt
     2461 tccacgtgga tatcagaggt atctaaagag attgcggcca caaggagggt atgaaaaaaa
     2521 ggagttatac ccgttggttg ttggagctgc tggagcttct ctcggagttg ctctaagttt
     2581 cggacgctct ggcgagtttc ctcgagaagg gagggattga tgtggattaa gcccgtcagt
     2641 tcttcgcgtg cttctggcgg gttttccgat gggggtgtgt tatgagagga gggcataggt
     2701 aagttggtgt atggaagtgt taggaagaga aagtaaaatc cagtttccct tttcttgggt
     2761 ttgtcctgct ccgtggagca gttttttgac tgcggggttt ttggtccacg caaggccttg
     2821 ggggagtgtt gaaggaaaag cttggaataa aaagaggtaa gatgcctgtt ctgaaaaagt
     2881 ctgctggagt tcttccaaaa gggcaacaag agttttctgc tccaccctgc tctctgcgaa
     2941 atcttctggg gcaagagaag taaggtaaag gtcaagtcgc tctgcaaact gcagcttgcc
     3001 gagaatgtgt ccgaacattt tgtgctctcc gttttcatgg gtggttaaga gttcaaagag
     3061 tgcgtctctt gcttcttctg cccaaagaaa agaagcaccc gcgtagggaa acaggttgtt
     3121 tttttgggac ttctccccta caatgatggt aagatccgtt tggttctgga cctgcagggc
     3181 aagagcttca ggcgaaactt ctccatcttg caacgtgaag tagagcttta catctttttc
     3241 gcttttttca tactggattt tcgatatcca gggggagata ccctttaaag aaacaacaaa
     3301 tgttttcttg tggtctacag aaggggaaaa gagattttcc cggaggccgt ttccctgctg
     3361 gactgttgct gtttttccga gttttttgag accggcgaga agaacgaggg aaagatgagt
     3421 atctcctaaa gttgcctctt tggaaaggag aataagaata ttttttgagt gcgaaagaag
     3481 tgctgttggc tccatgtatt ttaaaattag ctgatactgc cttaaaagtc aatgagtttt
     3541 ttttcgaaag atcctatata tacccactct accttggaaa caaaggcttt gggcgcactg
     3601 gttgcaaagt cgttcctcca aggaactaaa gaaactaatc gtgcggttgt gctggcgctt
     3661 gagggagagc tggggtctgg caaaaccacg ttcgttcagg ggtttgccaa agcacttggc
     3721 atacaagaaa acgtattgag tccaacgttt gtgattgtga gagagcatgg aaagctctac
     3781 catattgact gctaccgttt atcgtcagga gaagaattga aagcgctggg gtgggacaag
     3841 atggtgtccc acccaggttc ttttgtactg gtagagtggc cagagaacat acgagaacta
     3901 ttgcctcctt acaccaaaac gatttctttt gcgacattgg agggtgaaga aagagtgatt
     3961 tcgtttcgct aacccatgaa acccaaaacg cttctcctta ttgacgccaa ctctctttta
     4021 cacagggcgt tccacgccct gccgcctttg agcacgccaa aaggggaaat ggtgcatgcg
     4081 gtgtacggat ttctgtccat tatgtttaaa gcgatcaagg agtttcagcc gtcttatatt
     4141 gccgccgcgt tcgatgtggc ggctcccacg aaaaggcata agaagttcac gcagtacaag
     4201 gctacgagag taaaggctcc agacgagctc tatagccagt tccctttggc gaaagaagct
     4261 cttgcagcgt tccacatccc tgtgtacgaa aaagaagggt acgaggcaga cgacattata
     4321 ggtacgctgg ccggcaaagc ctctggtatg cgctctgaac aacccattga aacaatcatt
     4381 gtgagcggag acttggatac tctgcagctt gtggataaaa acacgaaagt ctatacgatg
     4441 agaaaggggt tgcaagagac ggcgttgtgg ggacaggaag ccattgcaga aaggtttggg
     4501 gggctaaaac ccgcccagtt gcttgactac aaggggttgc gaggcgatcc ttccgataat
     4561 attcccggag tcgcgggggt tggcgaaaaa acagcgttgc agcttatagg agagtttggc
     4621 tcgctcgaag ctttgtacaa cgccttagag aagaaaacat ctggtgcaca aacaataaaa
     4681 cccaagctta aagaaaaact cctttctgaa aaagaacagg cgtttttgag taaagagtta
     4741 gtgactattg aaaaagaagc gcccgtggat tttcacttag aagacttggc ttgggaggac
     4801 tacgaccaag aggcggcggc ggaattactt ttgggctggg attttcggtc tctggtccaa
     4861 agaatgccgg gtgagcgcag agaggggttg aaagaaggaa ggggctctca acgggatatg
     4921 ttcagagaga gcgtggagag caaaatcgaa aagttgcatg aggagcaggt tctttctaac
     4981 gaggtgtacg aactggaaaa aagacttacc cctgtgttgc ggaacatgga ggaaaagggc
     5041 ctgaaaatag acaaacctta ctttgcaaag ctggggcaag agatgcaaaa ggagatcgag
     5101 gtgtatgccc agagaattca tgagatggcc cgtaaaaact tcaacgtgaa ctctacccag
     5161 cagctttcag aagtgttgtt cggcttgccg cccgatggat tgggtatttc tctgaaaggg
     5221 ttaaagaaaa ctccaaaggg cgtagtgtct accgcttccc ccgaattgga aaaactccag
     5281 gaagatcacc cggtgattcg ggaggttttg tcttaccggg aattgcagaa gctttatact
     5341 acctacgtga aacctcttcc ccttttggcc gactctgaag gccggatcca tacgcatttc
     5401 gaccagctgg gagcggcaac gggcagggta tcttcgtctg aacccaacct gcagaacatt
     5461 cccaaccagg gagactgggg taggcggata cgcagagggt ttattgcgaa agaagggtac
     5521 aaactcgttt cttttgatta ctcgcagatg gagttgcgca ttgccgctca cattgcaggc
     5581 gacaagcgca tgcagcagtt ttttcgagag ggagcagaca ttcaccgtat gaccgcctcg
     5641 acggtatttg gcatggcccc cggagaggta accgaccaga tgcgctttcg cgcgaaagcg
     5701 ctgaactttg gcgtgttgta cggaatgggg gttcaaggat ttgccaaatc ggcgggaatc
     5761 ccaacccaag aggcccggga tttcattgag aattactttg tgcgttttcc ccaattgcat
     5821 gaatatatgg agcacatgaa agaatttgca cgtgaacatg gatatgtgga aacaataatg
     5881 gggagaagaa gatacatccc ggagatccac tctatagctc cgcaattgcg ctcggcagca
     5941 gaacgcatgg ctattaacca ccccattcag ggaagcctgg ccgatatagt taaaatggca
     6001 atggtaagga ttgaggagca acttcccgat ccccaaggga aacggagtat gctcttgcag
     6061 gttcacgacg aattgttgtt tgaaattgca gatggtataa tggaagaaat tatcgggcct
     6121 atcaggcgta ttatggagca ggcaggaaag ttggatgttc ctgtgcgagt cgatgtaaaa
     6181 aaggggaaga attggaacga tgtggaaaaa gttcttgcct aattcccgca gaaatggtga
     6241 gatagagtaa tcatgaagaa agttttggtt atcgaggacg aagcgcttat tgccgatctg
     6301 ttgtcgcggc ggttgcaaga gaaggggtat tacgcgtttc ttgcagaaga cggagaaaaa
     6361 ggacttcagt ttatcagaaa agaagcgccc gacctggtac ttttagatat tgtactgccg
     6421 cggctcaacg ggtttgaggt cttggaagaa ctgcaaaaag aggggctgct ttcgcggatt
     6481 ccggtaatta tcatttccaa ttccggacag cctgcagaga ttgaacgcgc caaagaagcg
     6541 ggcgtagtag actggcttat aaaaacagag tttgaccccc aggaagtaat tgaaaaggtg
     6601 gaaaaacaaa tcgggcccgg cgacctcgca atgcaaacat aaccatcata tatgtcaaaa
     6661 attctactca tcgaagacga caagttttta cgagagctga ttattcaaaa gctcgggaaa
     6721 gaaggctacg aagtggagga ggctcccgaa ggagagggag gccttacaaa gatgaaaagc
     6781 gggaagccag atttggtgtt gctcgactta attctccccg gcatcgacgg attcgaagtg
     6841 ttaagcaaaa tgaaagaaga tcccgagctg caaggcattc ccgtaattat tctttccaac
     6901 ctcggccagc gggacgacgt gcaaaaggga atcgacctgg gagcaaaaga ctaccttatc
     6961 aaggctcact ttactcccgg ggagattgtg gagaaaatca aagccacgtt gagcgccaaa
     7021 tgatcctgtt tttatacggt caggatactt atcgttcgcg acagaaggcg gcagaaattg
     7081 tgaaggaata tcaaaaagtc cggcctagag cagttcatgt gcgcgccata gactgctcaa
     7141 aagcggatgc agaagatctc aaaaacgaat tcgacacgat ttccctcttt gagcaaaaga
     7201 aattactctt gctgcgcgat cttttttcga acgccgaagt tgtccggttt ttcgacgaga
     7261 ggaaaaaaca actccttgaa agcagccacc atatagtggt tgttttgcag caggcggaac
     7321 tgaaagacaa aacaaagaac ccgctgtatc agtggcttaa aaaacacgcc acgtgccagg
     7381 agttttcgcc tttgcccccg gcaaaacttc ttttatggat tcagcgggaa ttcacgcgtt
     7441 acggcgtggc gtgcagttct cgggcagcag agctgcttgc gttgcgcgca ggaaacgacc
     7501 tgtggactct ttctcaagaa gtccggaaaa tagcggcgtt caaaaaatcc gaggccaaac
     7561 ctgcagtcaa ggagtctgac ataagccttt tggtggaggg caaagcccag gcagacgtgt
     7621 ttgccaccat agacgcttta gcttcgcgca acaaaaaaca ggcgttcgac ctgttgttgc
     7681 gccacctcca aaaaggggac tctgcgtact acctgttttc catgatgcat tatcaattca
     7741 gggtattgct ggaaattcgc gatatgctcg accgccaatt gtcttttgcg cagatggttt
     7801 ctgaaagcaa gctgcacccc tacgtattga aaaaaggggt tggggtggct caaaactttt
     7861 cttcccccca actcaaaaat atatatcaaa agctctttga ggtggacttt gccatgaaaa
     7921 ccggaaaagt ggaatccgaa ggcgctttcg atgtttttgt cgctagcctt taggggaatc
     7981 ctgttttaag agaaacttgg tgatccggga cttttttctt gaggccgcgt tctttttgag
     8041 aacgcctgtt tttgccgctt tgtctaaggc tttgtatacc tgcgaaagaa gctgttgggc
     8101 ttcctgctgc gttttggcca aagccaattt ccgcacctgc ttggtaaggg tctttacctt
     8161 attttttttc tttaaattcc gcgcctttct ttttgggttt tgtcgaagcg ccttgcgcgc
     8221 cgctttcgtg attggcatat ggcgatactg taccaccttc tatggatttt atcaagtccc
     8281 gaatctgggc ggctctttca aagtccaggt tttgggcggc ttgtttcatc tctttctcca
     8341 agagctttat gtcttgcacc gcgccaaact cggccgccgc tatctcttgt ttgctggcaa
     8401 aaccccagct tcgaatgtct tttacgatag cggttggcgt aatgttgtgt tctttgttgt
     8461 agttttcctg gattttgcgg cggcgttcca cctcttttat ggcctgttgc atagaaaatg
     8521 tagttttgtc tgcgtataaa atggctttgc cttctggatg gcgcgccgcc ctccccatag
     8581 tttgaataag cgtggtttgg ttgcgcaaaa atccctcttt gtccgcgtcg agaatggcaa
     8641 ctaaggcaac ttcgggcaaa tccaagcctt cgcgcagcag gttaatgcct acgataacgt
     8701 cgtacttgcc gcggcggaaa tcttccaaaa ttccggggcg atcgagcgtt tttacttcag
     8761 aatggagcca ctgcacggcg atgttccgct gcgccaagta atcggtaatg tcttctgaaa
     8821 ggcgcttggt taaggtaata agcaatacgc gagaaccttt ttgcttgtat ttttcaattt
     8881 ctaagataac atcttggatt tggttggtgg ttggcctaat ttcaattgcg ggttccaaaa
     8941 gcccggtggg ccggataatt tgctctgcga tatttttgcg gcctgcttct tctgtttcaa
     9001 agtcgccggg agtggcggaa atatatatgg tctggggagc gcgcttaaaa acttcgtcaa
     9061 acgtaagcgg gcggttgtcc acggcggaag gcaagcgaaa accaaactct acaagcgatt
     9121 gcttgcgcgc tttatcttgc accgacattg cccgcaactg ggataaggta aggtggctct
     9181 cgtcgataaa caccaaaaag tcttttccaa aatagtcgag cagcgtatag ggcggctcgc
     9241 cgggttggcg aaactccatg tgcgaagaat agttttcaat gccgtggcaa tagccggatt
     9301 cctcgatcat ctccaagtca tagttggtgc gttgctccaa ccgctgggct tccaccagtt
     9361 tgttttgcgc cttgagttcg tgtagccggt cttgcagttc tagccgtata ttttgcattg
     9421 cgattttaag cttgtcttct ggggtaaccc aaaactttgc ggggaaaaac cggtattttt
     9481 ctatgggaga atacgaggga gctacgctgt ttgcagaaac agaaaggctt tctatggtat
     9541 cgccaaccag ctccaaccgg ataagttctt ttcccgtaac cacgaagact tcaatagttt
     9601 ccccccgaat gcgaaaggtg cccggcttaa aatcaatatc gtttctttgg tactgtaagg
     9661 cggtaaggtg cgacaataat tttctgcggc cgatcacttg gccgacccgc aaatccaagg
     9721 caatgttttg gtaatcttcg ggcgagccaa tgttgtaaat gcaagaaaca gaagccacca
     9781 caatggcgtc tttgcgcaaa agtacatctt gcgctgcctc atgtcgcaag cggtctaagg
     9841 tgtcgttgat tttggcgtct ttgtctatgt aggtgtcgga ttgtggaata tatgcttcgg
     9901 gttggtaata gtcgtaatac gatacaaaat agtgcacgcc gttttcggga aaaaattcct
     9961 tgagctcctg gtacagctgg gcggccaggg ttttgtttgg cgaaattacc agggcgggct
    10021 tttgcgtgtt ggcaataaga tttgccatgg taaatgtttt gccgcttccc gtaacgccca
    10081 aaagcacctg gtgtttttcg ccagattgta tgccgcgcgt aagctgggca atggcttggg
    10141 gctggtcgcc cgtaggcttg aattttgaaa ccagtttaaa ttgcactata gtaaaggtat
    10201 gattagttat cttaagggaa atattttacg aaaggactct gattatatca tactccaaac
    10261 cgcaggggta gggtataagg tacatgttcc agaagtcgtg tttatgcagt gtaaagaagg
    10321 ccagcaggat tttgaggtgt tttgcgcgtt gcaaatccgc aaagatgaaa ccttggaact
    10381 gtacggcgtg cccacgttcg aagcgctcaa actattcgaa gcgtttcgcg acgtttctgg
    10441 cattgggcca aaagccgcaa tggtgctttc gtctctcggt aagccagaag acattaaaaa
    10501 agccatagac cagcgaaaca ctttgtattt taagggcatt aagggcattg gcgaaaaaaa
    10561 ggtgcaaaaa ctgattttgg aacttaccgg caagttgggc agttttgaaa gcggaaaaaa
    10621 gtcttttggc gaccccattg ttgactcttt ggtaaattta gggttttcca aagcagaagc
    10681 aaaagaagcc ctgctgcagc tgcccgcaga tgttaccgac cccgaagaaa aaataaaagc
    10741 cgccctaaaa ctgctcggcc gccgctaaca ttgcacaatt tgacagatgt ggtattatgg
    10801 gcgtggcaca aatctatcca aatttgggga agaaaggagg ttttatggct aaaaagccgt
    10861 accagccacc ccggctacgc cactatggcg gtctgccgga gttgacgcgg ggaatgccgc
    10921 atcctggagc cggaccccag gacaataacg gcaagatccc ggggggaggg gatgacctag
    10981 cctccgccat cctgtttgtt cccgggagtc cctttgttgt gcagccccgg taggcggcac
    11041 gaccagcaag gggcatacgc atgcgtgtgc ccctttatct ttgcccgcaa aattgacgaa
    11101 agtttttgaa caaatcatac taaaagtagc ccttaaaggg tagtttgttt caggaggtgg
    11161 aagatgaaac ttccaagagg tccaactttg ttgagactgc ttattatggc actggcgctg
    11221 cttttggttc ttccggcagt tgctagtgct aaggagagtt ctgcggacgc tgctggttct
    11281 acgattacac ttcgttttag tgaagccaca accagcgaag gcggtactac aactgttaaa
    11341 gttgcggttg tcgaagagga aggatgggtt gtttctgaac aaactctcga ccttgggaga
    11401 agcgggcatc agctaaccct tctagtagac ggaacctcag tagttgtgag cacccatgtc
    11461 agaggggtta atcctcttag gtcaaacgag cttgctgcca aaatgcttac aataggatta
    11521 ttgacaacac aagggccgga acttagcccc ataggcataa gcgcagaaag tcgtgcgact
    11581 ttcgagggaa ttgaagtgca aggaggttca gtggccatgt cggcccagct tgtaggcacc
    11641 tgtgtgccat gccaccggta agtagtcctg cccttggact gcctgcggca cagggatcgc
    11701 gaagcgaccc tgcgcccttt atttttaggc aaaacattgt ttaatgtatt gagtttagcg
    11761 ctacaaggaa atatgatata ctaaaaatac taacggccgt gagtatttta cgtatatgtc
    11821 gaaatacaaa tactatctta aaaagccgaa aagcgaaatt acgaaagatg tt
//
LOCUS       DASRUP010000015         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2191045, whole genome shotgun sequence.
ACCESSION   DASRUP010000015 DASRUP010000000
VERSION     DASRUP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191045"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFE94_00740"
     CDS             complement(<1..291)
                     /locus_tag="VFE94_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZX49662.1"
                     /translation="MLQVYNTLSRKKELLKPRKGKKLNVFVCGVTTYDFSHIGHARTY
                     IAFDMIAKYLRHKEYDVFYLQNVTDIDDKIIKRAKEEGQSPLELARRFEKEYM"
     gene            390..767
                     /locus_tag="VFE94_00745"
     CDS             390..767
                     /locus_tag="VFE94_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49663.1"
                     /translation="MKKVDRDVLIIGFIKGIPGENPASVTALATDLYPELKDTSGIYN
                     NPKTVALRDSLKKGGSLYNAVDDYYTAKRRGRPVPTRFTEADVEILEALWQKYRSFHT
                     FRSVHEDLMTGGIHRHSAAKEKR"
     gene            826..1443
                     /locus_tag="VFE94_00750"
     CDS             826..1443
                     /locus_tag="VFE94_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZX49664.1"
                     /translation="MKKDILVAAIIGFLVALLLLVIKLTGNIDRLNRLPYVEYSLVAF
                     PLLAALGIAVASALGKRVRIVFQGAKFLLVGVLNTFLDLGVLNILTAATGVTGGILFA
                     VFKGFTFLLAVANSYLWNKYWTFSSRKGTSQDFVAQGKEFAQFLVISGVGFFLNVGTA
                     SAVVNFIEPQFGLSERAWPTAGALAGTLVVLTWNFLGYKLIVFKR"
     gene            1450..2562
                     /gene="dnaX"
                     /locus_tag="VFE94_00755"
     CDS             1450..2562
                     /gene="dnaX"
                     /locus_tag="VFE94_00755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZX49665.1"
                     /translation="MSNIALYRKYRPKSFAEVVGQEHIVQTLKNAVAGDLLSHAYLFS
                     GPRGSGKTSIARLLAKAVNCKNPKEGEPCNKCDSCQEINAGRAIDLIEIDAASNRGID
                     EMRELREGIAFAPSRLKYKVFIIDEAHQLTKEAANALLKTLEEPPAHAIFVLATTEAH
                     KMISTIASRCQRFDFRKLTVPEMVKKLQEIVKAENAKIDKEALQLIALNSGGAMRDAI
                     GLLDKVLTFHSVLDTKEGIGADAIKDLLGLVDMNLVGDFMQLLAEKKPGEAIDFLNKN
                     LESGMDPQEFAKNAVRYLRHTLVLKINPQLDKMFESELSREQLDRMKQQAEKFEEKAL
                     VQIVEHFMEAEQKMKYSSIIQLPLELAIVESTRVGD"
     gene            2598..2855
                     /locus_tag="VFE94_00760"
     CDS             2598..2855
                     /locus_tag="VFE94_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZX49666.1"
                     /translation="MERTQIYLSKSQVKKLKELAQKKKTTFSGLVRDAVDVQYGVEQP
                     AKWTKKEETLLDIAARVNALGEKGPGDLATNLDEYLYGGKK"
     gene            2839..3273
                     /locus_tag="VFE94_00765"
     CDS             2839..3273
                     /locus_tag="VFE94_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZX49667.1"
                     /translation="MEERNKVFADSNYFVALFNTDDSLYEEARHIAQKISSDATQLAI
                     SNFIFLEVVSILSQRKGKAAAIQAGQDILSHPSLINVVHVDETLQQAAWDIFQSIEHK
                     NISFVDCSIIAAIKTEGIKKLLTFDKTDFKKLQKKYRFSFYE"
     gene            complement(3283..3353)
                     /locus_tag="VFE94_00770"
     tRNA            complement(3283..3353)
                     /locus_tag="VFE94_00770"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3319..3321),aa:Gln,seq:ttg)
     gene            3426..>3648
                     /locus_tag="VFE94_00775"
     CDS             3426..>3648
                     /locus_tag="VFE94_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZX49668.1"
                     /translation="MYFVYIIECQDNTLYTGITTNVQRRFKEHSLGKGGAYTRSKKPK
                     KLLYTETHPNRSSALKRESEIKSWPRAKKL"
BASE COUNT         1071 a          825 c          834 g          918 t
ORIGIN      
        1 catatactct ttctcgaacc gccgcgccag ctcgaggggg gactgtccct cctctttggc
       61 gcgctttata attttgtcgt ctatgtcggt aacattttgc aagtaaaaca catcatattc
      121 tttgtgccgc aggtattttg cgatcatatc aaaagcaata taggtgcggg catgccctat
      181 atgcgaaaag tcgtaggtgg taacgccaca tacgaacacg ttcagctttt taccttttcg
      241 gggcttcagc agctcctttt tcctggaaag cgtgttgtaa acctgtagca taagtaaaag
      301 tattgtaaca tgcccctgcc ctttgcgcac gtctgtacag ttttaaaatt tatggtatta
      361 taaacaaaac tccacttgcg aggtttgtta tgaaaaaggt ggatagagat gttcttataa
      421 tcggctttat aaagggaatc ccaggggaaa acccggcttc cgttacagcc cttgcaactg
      481 atctgtaccc tgaactcaaa gacacctcgg ggatatataa caatccgaaa acagtagcgc
      541 tgcgagatag cttgaaaaaa ggcgggagcc tttacaatgc tgtagacgac tactacacgg
      601 caaaaagacg cggccgccca gtgccaacta gattcacgga agcagatgtc gaaatcctgg
      661 aggcgctctg gcaaaaatac cggagtttcc ataccttccg cagtgtacat gaagacctga
      721 tgacgggcgg tatacaccgg cattcggcag ccaaagaaaa aaggtagata ccacgaaatt
      781 tctacctttt tattttggct ggcatttgtg ctacaatgca aaagcatgaa aaaagatatt
      841 ctcgttgctg ccattattgg gtttttggta gcccttttgc ttctggtaat aaaactcacc
      901 gggaacatcg accggctaaa ccgcttgcct tatgttgaat acagcctggt ggctttccct
      961 ttgcttgcgg ccttaggcat tgcggtagcc tctgccttgg gaaaacgcgt tcgcatagtc
     1021 tttcaggggg caaagttttt gctggtaggc gtccttaaca cctttttgga ccttggggtt
     1081 ttaaacattc ttactgcggc caccggagtt accggcggta ttttgtttgc tgtgttcaaa
     1141 ggattcacat ttctgcttgc ggtagccaac agctacctct ggaacaaata ctggactttt
     1201 tcttccagaa agggcacttc gcaagacttt gtagcacagg gcaaagagtt tgcgcagttt
     1261 ttggttatca gcggggttgg gtttttctta aacgtgggaa ctgcctcggc cgtggtaaat
     1321 ttcatagagc cgcaatttgg cttgtctgaa agagcgtggc ctactgcggg agccttggca
     1381 ggaacattag tggtgctcac ctggaacttt ttgggataca aactcatcgt ctttaaacga
     1441 tgaacataca tgagtaacat tgctttatac cgcaagtata ggcccaaatc ttttgccgag
     1501 gttgtaggcc aagaacacat tgtgcagaca ttaaaaaacg cggtagctgg cgatttgctt
     1561 tctcatgcgt atttgttttc aggaccaaga ggctctggca aaaccagcat tgcgcgcttg
     1621 ctggcaaaag cggtaaactg caaaaatccc aaagaaggcg agccctgcaa taaatgcgat
     1681 tcatgccagg aaattaatgc gggccgcgcc atcgacttaa tcgagattga tgcagccagc
     1741 aataggggaa tcgacgaaat gcgtgaactg cgagagggca ttgcgtttgc gccctcgcgg
     1801 ctcaaataca aagtatttat tatcgacgag gcgcaccagc ttaccaaaga agccgccaac
     1861 gccttgctca aaacgttaga agagcctccg gcccatgcta tttttgtgct ggctaccacc
     1921 gaagcccata aaatgatttc caccatagct tcgagatgcc agcgttttga ttttcgaaaa
     1981 ctcaccgtac cagaaatggt aaaaaagctg caagaaattg taaaagcaga aaatgccaaa
     2041 atcgacaaag aagccctgca gcttattgct ttaaacagtg ggggagccat gcgcgacgcc
     2101 attggcctgc ttgacaaggt tttgactttt cactctgttc ttgatacaaa agaggggatt
     2161 ggagcagacg caataaagga tttgcttggc ctggtagaca tgaacttagt gggcgacttt
     2221 atgcagctgt tggcagaaaa aaagccgggt gaggctatag actttttaaa caagaatctg
     2281 gaaagcggca tggacccgca ggaatttgcc aaaaacgcgg tgcgctattt gcgccatact
     2341 ttagtactca aaataaatcc gcagctcgac aaaatgtttg aaagcgagct aagccgagaa
     2401 cagttagacc gcatgaagca gcaggctgaa aaattcgagg agaaagcgct cgtgcagatt
     2461 gttgaacact tcatggaagc cgagcaaaaa atgaaatact ccagcattat ccagctcccc
     2521 cttgaactcg ccatcgtgga atccacgaga gtgggggatt gacatgcata tacaccatgg
     2581 tgtatactga aattactatg gaacgaactc aaatttatct ttccaaaagc caggtcaaga
     2641 agcttaaaga gcttgcccaa aagaaaaaaa ccacattctc cggactcgta agagacgcgg
     2701 ttgatgttca atatggggtc gagcaacccg ccaaatggac caaaaaggaa gaaacgttac
     2761 ttgatattgc agctcgcgtg aacgcactcg gagaaaaagg cccaggcgat cttgctacca
     2821 atctcgacga atatttatat ggaggaaaga aataaagtat ttgccgactc caactatttt
     2881 gttgctctct ttaatacgga cgattcgctt tacgaagaag ctcgtcacat tgcgcaaaaa
     2941 atctccagcg acgcaacgca gttggcaatt tcaaacttta tctttctcga ggtagtcagt
     3001 attttatccc aaagaaaggg aaaggccgct gcgatccaag cggggcaaga cattctctca
     3061 cacccctcct taatcaacgt ggtgcatgtt gatgaaacgc tccaacaggc agcttgggat
     3121 atattccaaa gtattgaaca taagaacatt agttttgttg attgcagtat tattgcagcg
     3181 ataaaaacgg aaggaattaa aaaacttcta acgttcgaca aaaccgattt taaaaaactg
     3241 caaaagaaat accgattttc tttttacgaa taggttttaa tgctgcggga cttggattcg
     3301 aaccaagata ctcgcctcca aagggcgatg tcctaccatt agacgatccc gcaaaattcg
     3361 cgatacgatt taaactatac cacaattttc ccataaaatc cagcccttat gcggctggta
     3421 cccttatgta ttttgtttac attatagaat gtcaagataa tactttgtac acaggcatta
     3481 caaccaatgt gcagcgcaga tttaaggagc atagcctcgg aaagggcggc gcctacaccc
     3541 gctccaaaaa accaaagaaa cttttgtaca ccgaaaccca tccaaacaga agtagtgctt
     3601 taaaacgcga aagcgaaata aagtcctggc cccgcgccaa aaaactca
//
LOCUS       DASRUP010000016         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_198442, whole genome shotgun sequence.
ACCESSION   DASRUP010000016 DASRUP010000000
VERSION     DASRUP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198442"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..553
                     /locus_tag="VFE94_00780"
     CDS             95..553
                     /locus_tag="VFE94_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZX49669.1"
                     /translation="MKPFFVVLDNIRSAENVGSIFRTADALVVDKIFLCGITPTPAHE
                     KVGKTALGAEKTVVWEYHKQTWRLLEKLKEQGVFIAALEQTKKSVSLEKFKPKFPLAL
                     VLGHEVKGVSPSVLRLANAIVEIPMLGQKESLNVAVAFGIAGYEINKVRL"
     gene            559..630
                     /locus_tag="VFE94_00785"
     tRNA            559..630
                     /locus_tag="VFE94_00785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:592..594,aa:Arg,seq:ccg)
     gene            632..1768
                     /locus_tag="VFE94_00790"
     CDS             632..1768
                     /locus_tag="VFE94_00790"
                     /inference="COORDINATES: protein motif:HMM:NF015197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HZX49670.1"
                     /translation="MNNRFLLALATLVGTIIGAGIFGLPYVISKSGVLPGLFYFVVLG
                     GVVLLLHLMFGEIALRTKEKRRLIGYAEVYLGGWAKKVATISTVVGIVGALLAYIILA
                     GDFLTLLLSPMLGGNALVYSLAFWLFFSLLVLRGIQTIARVELLMNGALFAAIALIFL
                     FALPHIQLENFPVFDAAYLFLPYGVVFFAFTGLSAIPEIAELFKGRNEKRRLDNLIVY
                     SSLLVGLVNLLFVIAIVGVTGGQTSSNALGGVVPIVGTGIGTLGVFFGLLAVATSFLV
                     LGNYLKNSLRYDFRLPYAFSVAIALGAPLVLFLLGLREFILVIGIIGAFMAVLDGGLV
                     VLIHRKAARQGNRTPEYRLRIPQAVAWAVLVFLLAGALAEILIL"
     gene            1901..2191
                     /locus_tag="VFE94_00795"
     CDS             1901..2191
                     /locus_tag="VFE94_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZX49671.1"
                     /translation="MAEKARTHIFVSGRVQGVLYRRGAQKKAQALGITGFAHNLADGR
                     VELMAEGDKEGIEQLMKWCKEGTPMAKVDRLDSQFEDYKGEFADFEIREFGF"
     gene            2195..2722
                     /locus_tag="VFE94_00800"
     CDS             2195..2722
                     /locus_tag="VFE94_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZX49672.1"
                     /translation="MKAFWKRFRQGAYIGDFVYGANDGIVTTFAVVAGAAGALLSPGV
                     IIILGFANLLADGFSMGASNALAIRAEHELAKKERDLVTPFQHGLVTFVAFLVAGLMP
                     LLPYLFSFSSGQQFFISSALAGFAFFCVGAARTLITKGNSLKAGFEVLVIGGIAAFVA
                     YGMGWAIKTAFGIVI"
     gene            2723..3460
                     /locus_tag="VFE94_00805"
     CDS             2723..3460
                     /locus_tag="VFE94_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49673.1"
                     /translation="MTADFFKQKFGYEIDGVWFPRVTSITSPFQKKWFGSFGAAAWGT
                     SMHETIEALLKGERVLFDPKAATAIQTFQEWQKVYPLRILDPQKDIERRVVNLEQGYA
                     GTIDMVAEVDGVFGIIDLKTSTSILREHAIQTAAYFHAYNATKGVQRACETRWILRID
                     QYQECRGCFAKSREKYGAARISGGKEVCNHQWAELKGEIEFKELPGADKDFEDFLSMK
                     ERWEWANKEWLDKIPNYEKNIRQYTLL"
     gene            3605..>3840
                     /locus_tag="VFE94_00810"
     CDS             3605..>3840
                     /locus_tag="VFE94_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49674.1"
                     /translation="MKYSHSFPLAVFVAACIAGTLSGGFLLQPRVSAAQAIPVGSSFH
                     YRFLMPGILNEAGDMEDTWSPYWWVNSGAHLILE"
BASE COUNT          906 a          770 c         1050 g         1114 t
ORIGIN      
        1 cttcggattc tattggcatg aactttattc tcgacgacat cgaaaagcag ggtgtggaaa
       61 ttgttccttg ctcggggctt tttcgtgtgt ctcgatgaaa ccgtttttcg ttgttttaga
      121 caacattcga agcgcagaaa atgtcggctc gatttttcgc acggcagacg ccttagtcgt
      181 agataaaatc tttctctgcg gcattacgcc tactccagcg cacgaaaaag tggggaaaac
      241 cgctttgggc gctgaaaaaa ccgtggtatg ggaataccat aagcaaactt ggcgtttgct
      301 cgagaagctc aaggaacagg gtgtttttat tgcagcttta gaacaaacaa aaaagagcgt
      361 gtcgttagaa aagtttaagc caaaatttcc tttagctttg gtgcttggcc acgaggtaaa
      421 aggcgtgtcg ccttctgtgt tgcgcctggc caatgctata gtagaaatac ccatgctggg
      481 gcaaaaagaa tcgctcaacg tggccgtagc attcggcatc gcaggttatg agataaataa
      541 ggtacgatta taaatgaagc cctcgtagtt taacggatag aacgcgagat tccggatctt
      601 gtaatagggg ttcgattcct ctcgagggca catgaacaac aggtttttgt tggcgctggc
      661 aacgctggtg ggcacgatta tcggggcggg gatttttggg ttgccgtatg taatatcaaa
      721 aagcggggtt ttgccagggc ttttttattt tgtggtgctg ggaggagtag tgttgctttt
      781 gcacttaatg tttggcgaaa tcgcgctgcg cacgaaagaa aaacgccgct tgataggtta
      841 cgctgaagta taccttggtg gctgggcaaa aaaagtagcg accatttcta cggttgtggg
      901 aattgtcggc gcgttacttg cctatattat tttggcagga gattttctta ctcttttgct
      961 ttcccctatg ttgggaggaa acgcgctggt gtattctctg gcgttttggc ttttcttttc
     1021 tttgctagtg cttcggggaa ttcagactat cgcgcgagtg gagctgctca tgaatggagc
     1081 gctttttgcc gcaattgcct tgattttttt gtttgctttg cctcatattc agcttgagaa
     1141 ttttcctgtc ttcgacgctg cctatctgtt tcttccttat ggcgtggtat tttttgcctt
     1201 tacaggattg tctgcaattc cggaaatcgc ggaattgttt aaaggcagga atgaaaagag
     1261 aaggttggac aatcttattg tgtactcgtc gttgctggtg ggtttggtga accttttatt
     1321 cgtaattgcc atagtagggg ttacaggcgg gcaaacgtcg agcaacgctt tgggcggggt
     1381 cgtgccgatt gtagggacag gaataggaac gctgggggta tttttcggct tgttggcggt
     1441 tgccacctct tttttggtgc tggggaatta ccttaaaaac tctcttcgct acgattttcg
     1501 cttgccctat gcgttttctg tggcgattgc tttgggagcg ccgcttgttc tattcttgct
     1561 gggactccgc gaatttattt tggtaatcgg tatcatagga gcctttatgg ctgtgctcga
     1621 cggaggtctc gttgtgctta ttcaccggaa agccgcaagg cagggaaatc gcacccccga
     1681 gtaccgtttg cgtattccgc aagctgttgc ttgggccgtt ttagtttttc ttttagctgg
     1741 cgctttagct gaaattttga tactataata gaactatgct ggacattatt ctttcgattg
     1801 tggtggttgg cgctattatt gtgtttattc tttgggcgtt tagcgccctc gggctccctt
     1861 tgttatcaga tattccaggc ctgtttcagt aaatgttcat atggcagaaa aggctcgcac
     1921 acacattttt gtttcaggaa gggtgcaggg cgttctctat cgaagaggcg cgcagaaaaa
     1981 agcgcaagcg cttggtataa cgggctttgc gcataacctg gcagacggca gagtagagct
     2041 tatggcagag ggcgacaaag aaggcattga gcaacttatg aaatggtgca aagaaggtac
     2101 tcccatggca aaagtagacc gcctcgactc gcaatttgaa gattataagg gagaattcgc
     2161 ggattttgaa atccgtgaat ttggatttta atctatgaaa gcgttttgga aaaggtttcg
     2221 gcaaggcgcg tatatcggcg actttgtcta tggggcaaac gacggtattg ttaccacatt
     2281 tgccgtagtt gcgggagccg cgggagcgct tctctcaccc ggcgtaatta ttattttggg
     2341 gtttgcgaac ctgcttgccg acggcttttc tatgggagct tctaacgccc ttgccattcg
     2401 tgcagaacac gagctcgcaa aaaaagaacg cgatctcgtc acgcctttcc agcacggcct
     2461 ggtaacattt gtagcctttc tagttgccgg gcttatgccg cttctccctt acctatttag
     2521 cttctcttcg gggcagcagt tttttatatc gagcgcgctt gcaggattcg cctttttttg
     2581 cgtaggggca gcgcgtacgc tcattaccaa gggaaactct ttaaaggcag ggtttgaagt
     2641 attggttatc ggcggcatag cggcttttgt ggcctatgga atgggctggg caataaaaac
     2701 agcgtttgga atagtaatat aaatgacggc agattttttt aagcaaaaat tcgggtatga
     2761 aatagacggc gtatggtttc cgcgcgtaac ttcgattact tcgcctttcc aaaagaagtg
     2821 gtttggttct ttcggggcgg cagcatgggg tacttctatg catgaaacta ttgaggctct
     2881 cttgaagggc gagcgcgtgt tgtttgatcc caaagctgcc accgccattc aaactttcca
     2941 ggaatggcaa aaggtctatc ctttacggat tctcgacccc cagaaagata ttgagcggcg
     3001 cgtggtaaac ctggaacaag ggtatgcagg aaccattgat atggtggcag aggtcgatgg
     3061 agtatttggc ataattgact tgaaaacgag cacctccatt ttgcgggaac acgccattca
     3121 gactgcagca tattttcatg cgtataatgc caccaagggg gtgcaaagag cttgcgagac
     3181 aagatggatt ctccgcattg atcagtacca agagtgcagg gggtgttttg caaagtctcg
     3241 cgaaaaatac ggggcagcac gcataagcgg aggcaaggag gtttgcaacc atcaatgggc
     3301 agagttaaaa ggggaaattg aatttaagga gttgccgggc gcagacaaag actttgagga
     3361 tttcctgagc atgaaagagc gctgggaatg ggcaaacaaa gaatggctgg ataagattcc
     3421 caactacgaa aagaacattc gtcaatacac gttgttgtaa aagagggggt tgtggaaaac
     3481 cctgtggaaa aacctgggga aaacttttat gggatatggt tgacatctaa ggctcggctg
     3541 ataaaataga acacgatgtt tccgaaaatt gcgtgcctag aacgcaattc gagggagtcc
     3601 cgtaatgaag tattcgcata gttttccatt agcggtgttt gtcgccgcct gtatcgcagg
     3661 gactctctcg ggaggatttc tcttgcagcc tagagtgtct gcagctcaag ccatccctgt
     3721 tgggtcttct tttcattatc gttttcttat gcctggaatt cttaacgagg caggggatat
     3781 ggaagatacc tggagcccgt attggtgggt taattcgggt gcgcatctta tcctcgaaaa
//
LOCUS       DASRUP010000017         6528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_724083, whole genome shotgun sequence.
ACCESSION   DASRUP010000017 DASRUP010000000
VERSION     DASRUP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724083"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(533..1414)
                     /locus_tag="VFE94_00815"
     CDS             complement(533..1414)
                     /locus_tag="VFE94_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZX49675.1"
                     /translation="MVHQKILIAMVGLLIALAGGVFVLLSQGNPPAGGEIAKKALVAS
                     VQESLSQENSQGGAQEESEVFEVKLQTEELGPEPSPVPKLAPSPTPQNPAPAPTLLPE
                     ADAQAETPAPQPSPVPVPTTTPETPSPSPSSPAPQPQPQPQPAPSPQPAGKININTAG
                     LEELDHITGVGPAIAQRIIGYRTNVSLFYVIEDIKNVSGIGDVTFEKMKDEITVGDIG
                     PAPIPQSSPPPPAPSPAPQPSGHTFYTSSYHSSKLYYCDTDSVWESLSATYLESYPSE
                     EAVLAVYPEKTLHEPCE"
     gene            complement(1434..2144)
                     /locus_tag="VFE94_00820"
     CDS             complement(1434..2144)
                     /locus_tag="VFE94_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZX49676.1"
                     /translation="MKRLGLTILVGCSLFCLGATGGIWAQAFLMPALASSDTFAAVPF
                     VKEWSARTIIIREVQEMLITRDQAAQQVAQRGQSLVVSVQTGSGLILTSDGLVLTLAE
                     LVPAQNSVSVVLESADNPLAAQVLKRDTAANLALLKIDRNNLSTVGFESSAALGQDLV
                     MVARVVEGGELIAITNQGTVRTVENDIIRTNMFDKASLKGSALFDLEGNIVGVVNIEQ
                     GGRVVAIPASAIRAFTGF"
     gene            complement(2152..4872)
                     /locus_tag="VFE94_00825"
     CDS             complement(2152..4872)
                     /locus_tag="VFE94_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX49677.1"
                     /translation="MMQGPGNFTHKAQEAILQAQNIAQERGQQQVDALHLLFSLLLQE
                     DSVVITLLQKVGADIDGLKRKTELALTRLPVSASPTPFGQFYLTQDLGKALERARQES
                     VKMGDEYISVEHLFLALLDTDSKAKEILERTSLLPMEGGDAAVLEFGHLTYEAALKIL
                     AQIRGGARITDPSPESKYQVIEKYARNLTRLAQQGKLDPVIGRENEIRRLMQVISRRT
                     KNNPVLIGEAGVGKTAVVEGLAQKIIKGDVPESLKEKEIIALDLGALVAGTKYRGEFE
                     DRIKALLKELQRAGGKYLLFIDELHTLVGAGAAEGAIDASNLLKPALARGELRAIGAT
                     TLKEYQKYIEKDPALERRFQPIYVAEPSIEDAILILRGIKEKYELHHGVKIKDSALVA
                     AANLAARYVTDRFLPDKAVDLIDEAMSSLRLDMESEPVDLESLKREIQKLEIEREAFK
                     GDKSEERQMKVANRQLADLQEKAKAIEAKWVAEKEVVNKIKEFKKAVEEARFQVEREQ
                     TAGNLQRVAELKYGELPELLKKLRAEERKLSRYQKSHPILKEEVGEEDVARVISRWTG
                     IPVTRLIEEEARKLEKMEDVLKKRIIGQEEAIGAISNAIRRSRAGISEENRPLGSFMF
                     LGPTGVGKTETAKALAGFLFNDENALVRLDMSEYMERHTVSRMIGSPPGYVGYEEGGQ
                     LTEKIRRRPYSVVLLDEIEKAHPEVFNMLLQILEDGRLTDAKGRVASFKNAIIIMTSN
                     IGSQYIAEMSPLGFFGGRSPEEEKKASVRDRVMVALKEQFRPEFLNRVDEVIIFDYLE
                     KEQIKKIVELEMAKVSQRLRERGIELEVAEQAKGVLAERGFDSNLGARPLKRVIQKLV
                     LNPLALKIVSGEITAGGKARIDVEEGEIVVQGMYVMPHLKKEKLAKAEAK"
     gene            complement(4913..6181)
                     /locus_tag="VFE94_00830"
     CDS             complement(4913..6181)
                     /locus_tag="VFE94_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZX49678.1"
                     /translation="MEFQSPDYPQNATIQRPLWGVVFVSAVVGFLAGGAAGGYAYWQV
                     TQQLEEAGIAVSSTDSNGSAVQVDQELKVIDVVKEVSPAVVSVILTQDVPVYERVLRD
                     PFEDLFGGPSPFSIPELQQRGTERREIGGGSGFLVSADGMVATNRHVVSQDNVEYTVF
                     LRDGTSYDATVLATDPLQDLAFLKIERAEPFPFVKLGDSDALEIGQSVVAIGNALGEF
                     QNTVSVGVISGLGRTITASDGGSLVETIEDVIQTDAAINKGNSGGPLLNLSGEVVGVN
                     TATVLQAQSIGFAIPIEGVQRDLYQMANGGEIEYPFLGVRYVPVTKQLQEEESLPVDY
                     GVFVTSGSSQPAVTPDSAAQKAGLKEGDVIVEIDGQRVMQENSLGKILRQYAKDRQPP
                     IYRPGDTIALKVFRDGAEITLSATLGERPE"
     gene            complement(6192..>6528)
                     /locus_tag="VFE94_00835"
     CDS             complement(6192..>6528)
                     /locus_tag="VFE94_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZX49679.1"
                     /translation="IKGVLRRIPDSKLTGSAERRLANNAHFRFRGVEGRDVVVLLDQK
                     GIAVSTGSACSEKTQEPSHVLLALGLSSRDALSAVRLSLGKPTRLEDIEKTIKVLAWS
                     VASLRKAKV"
BASE COUNT         1508 a         1795 c         1486 g         1739 t
ORIGIN      
        1 cttttcgtct gcgggtaagg catccgccac tcgatgagcc gcaaaagcaa gacgcacaaa
       61 aaagtttcgg ttcattactt gaagatcact tttccacagt atatcagaag caagggggtg
      121 ggtcaacgaa acccttgaca aagctctaaa gatatggtat atagaaccca gctcgagagg
      181 agtcgcagtt cttaaatttt tagcagagag gtaatcatgg aagtaatgcc ttttgcggta
      241 tcctataaca cgcccgcacc acttggagga cctggaatcc gacatcttct aagacatgat
      301 agtcttgaag aaccgattaa cattacggga ttgggggatg ttgttaatgt tgtccccacc
      361 tcgaccccta ccaaccttca agagatagaa aatctcaaca agcaaggcct cgagatggct
      421 ccgaaaggca atctcgttat tatctatcgc ccttacttct aatcgcacag cctctcttga
      481 gcaaaacaaa acccctgact ttttaagcca ggggtttaac tatttacagg tattattcac
      541 agggttcgtg aagggttttt tctggataca ccgccaaaac tgcttcttca gatggatacg
      601 attcaagata ggttgcagaa agactttccc acacagagtc agtatcgcaa tagtataatt
      661 tagaagagtg atagctgctt gtataaaacg tatggccaga gggttgtggg gcaggagatg
      721 gagctggtgg tggaggtgac gactgtggaa tcggcgcggg ccctatatcc cccactgtaa
      781 tctcatcttt cattttctcg aacgttacgt ctccaatgcc gcttacattt tttatatctt
      841 caatcacata aaacaagctt acgttggttc ggtagccaat aattcgctgg gcaattgccg
      901 ggcctacacc tgtaatatga tctaactctt ccaaacctgc cgtatttatg tttattttcc
      961 ccgccggttg aggtgagggc gcaggctgcg gctgcggctg cggttggggc gcggggcttg
     1021 atggagaggg tgatggggtt tcgggcgtcg tcgtaggtac cgggacaggc gatggctgtg
     1081 gtgctggagt ttccgcctgc gcatcagcct caggcagaag cgttggcgca ggggcaggat
     1141 tttgcggtgt tggcgaaggt gctagtttag gcaccgggct cggttcgggg cctaactcct
     1201 ctgtttgaag cttgacttca aacacctcac tttcttcttg tgctccccct tgtgaattct
     1261 cttggctcaa actttcctga acactcgcca ccaaagcctt ttttgcgatt tctccgccag
     1321 ctggcggatt cccctgactt aacaaaacaa aaactccccc tgctaaagca ataaggagcc
     1381 caaccattgc aataagtatc ttctgatgca ccattcctta ctttatcaca ttttcaaaac
     1441 ccggtgaagg cgcggatggc agaggctggg atggcgacta cccttccgcc ttgctcaata
     1501 tttacaacgc ccacaatatt cccctccaag tcaaaaagcg cgctgccctt gaggcttgct
     1561 ttgtcaaaca tgttagtacg aataatgtcg ttttctacag tgcgaaccgt accctggttc
     1621 gtaatggcaa taagttcgcc cccttccacc acgcgagcca ccattacaag gtcctgaccc
     1681 aaagcagccg aagattcaaa accaaccgta gacaagttgt tacggtctat tttgagcaag
     1741 gcaaggtttg ccgctgtgtc tctttttaat acctgggctg ccaaaggatt gtctgcgctc
     1801 tcaagaacta ccgacacgct gttttgagcg ggcacaagct ccgctaacgt aagcacaagc
     1861 ccgtctgaag taaggatcaa gccagaaccg gtttgcacgc ttaccaccag actttggcct
     1921 ctctgcgcca cctgttgcgc tgcctggtct ctcgtaatga gcatttcctg aacttcacgg
     1981 ataatgatag tgcgcgctga ccattctttt acaaagggca ccgctgcaaa cgtatcgctc
     2041 gaagctaacg caggcatcaa aaaagcctgg gcccaaattc cgcccgtggc gcccaggcag
     2101 aacaaactac acccaaccag aattgttagt cccaaccgtt tcatgaatgc attattttgc
     2161 ttctgccttt gcaagctttt ccttcttgag atgcggcatg acatacatgc cctgaaccac
     2221 gatctctccc tcttccacat caatgcgcgc ttttccgcct gccgtaattt ctccggaaac
     2281 aatctttaac gccaagggat ttagcacaag cttttggata actcttttga gcggtctggc
     2341 gcccaggttg gaatcgaacc cgcgttcggc taacacgccc tttgcctgct ctgccacctc
     2401 aagttcaatg cctcgttccc ttaagcgctg ggaaaccttg gccatttcca gctccacaat
     2461 tttcttaatc tgctcttttt cgagataatc aaagatgata acctcgtcga cccggttgag
     2521 aaattcgggg cggaattgct cttttagcgc aaccataacc cggtctctca cggaagcttt
     2581 cttctcctct tcggggcttc tcccgccaaa aaatcccaat ggactcattt ctgcaatata
     2641 ctgggatccg atattcgaag tcataatgat aatggcgttt ttaaacgaag ctactcttcc
     2701 tttggcgtcc gtaagcctgc cgtcttccaa aatctgcagc agcatattaa acacttcggg
     2761 gtgcgccttt tcgatttcgt cgagcagcac cacgctgtag gggcgccgcc tgattttctc
     2821 ggtaagctgg ccgccctcct cgtaccccac atacccggga ggagaaccta tcatgcggga
     2881 aaccgtgtgg cgctccatat attctgacat gtccaaacga acaagagcgt tctcgtcgtt
     2941 gaacaaaaac cctgccaagg cctttgcggt ttcggttttt cccacaccgg tggggcccaa
     3001 aaacataaag gatcccagag gccggttttc ttctgaaatg ccggcccggc tgcgccgaat
     3061 ggcgttggaa attgctccaa tagcctcttc ctggccaatg atgcgctttt ttaacacatc
     3121 ttccattttt tccagtttcc tggcttcctc ctcgataagg cgggttacgg gaatgccagt
     3181 ccacctcgaa atcacgcgcg ccacgtcttc ttcgcccacc tcctctttta agatggggtg
     3241 gctcttttgg tatcgcgaaa gctttcgttc ttctgcgcgc agttttttca gaagttccgg
     3301 caattcgccg tactttaatt ctgcaacgcg ttgaaggttt cctgctgttt gctcccgctc
     3361 gacttgaaaa cgggcctcct ctaccgcctt tttaaattct ttgattttgt ttaccacctc
     3421 tttttccgcc acccatttgg cctcaatggc ttttgccttt tcctgcaaat ctgcgagctg
     3481 gcgattggct actttcattt gcctttcttc gcttttatca cccttaaaag cctccctctc
     3541 gatttcgagt ttttgaattt ctctttttaa agactccagg tccactggtt cagactccat
     3601 gtcgagccgc aaagaactca ttgcttcgtc aataaggtct accgctttgt ctggcaagaa
     3661 acggtcggtt acgtaacgcg cggccaggtt ggcagctgcc actaaggcgg aatctttaat
     3721 tttcacgcca tgatgaagtt cgtacttttc cttaatgccg cgcaaaatca aaatggcgtc
     3781 ttcaatgctg ggttcagcca cgtaaatggg ctggaacctg cgctctaaag ccggatcttt
     3841 ttcgatatac ttttgatatt ccttgagtgt ggttgctcca atggcccgca gttctcctct
     3901 agccaaggca ggcttcaaaa ggtttgaagc gtcgatagcg ccttctgcgg ctcccgcacc
     3961 cactaacgtg tgcaactcgt cgataaacag caaatacttt cctcctgccc gttggagctc
     4021 ttttaacaaa gctttaatgc ggtcttcaaa ttctccgcgg tacttggtgc cggcaaccag
     4081 agcgcccaag tccaaagcga taatttcttt ttctttcaag ctctctggca cgtctccttt
     4141 tatgattttt tgcgccagac cctctaccac cgctgtcttt cctacgccgg cttccccgat
     4201 gagcaccgga ttatttttgg ttcggcggga aattacctgc attaagcggc gaatttcgtt
     4261 ttctctgccg attaccgggt ctaacttgcc ctgctgcgcc aaacgcgtta agttccgggc
     4321 gtatttttct ataacctgat atttggattc cggggaagga tcggtaatgc gggctccgcc
     4381 gcgaatttgg gctaaaattt tgagcgccgc ttcgtacgtt aaatgcccga attcaagaac
     4441 agcagcatcc cctccttcca tcggaagcag agaggttcgt tccaaaatct cttttgcttt
     4501 ggaatccgta tcgagcaggg ccaaaaagag gtgttccacc gaaatatact cgtcgcccat
     4561 tttcaccgat tcctggcgtg cgcgctccaa ggccttgccc aaatcctggg taaggtaaaa
     4621 ctgcccaaaa ggggttgggg aggcggatac gggcaaacgg gtaagtgcca gttcggtttt
     4681 tcgctttaag ccatcaatat cagctcccac tttttgcagc aacgtgataa ccacgctgtc
     4741 ttcctgcaag agaagcgaaa aaagcaggtg cagcgcgtct acctgctgct ggccgcgctc
     4801 ttgcgcgatg ttttgcgctt gcaggattgc ctcctgcgct ttgtgggtaa agttaccagg
     4861 tccttgcatc atatggttat gttataacac gaaaattgtt tacaagcaag ttttattcgg
     4921 ggcgttcacc aagagtcgca gacaacgtaa tttcagcgcc gtctctgaaa accttaagag
     4981 caatggtgtc gcccggtctg tatatgggcg gttgccggtc ttttgcgtat tgcctaagaa
     5041 ttttgccgag tgagttttct tgcatgacgc gctgcccatc tatttcaaca ataacatctc
     5101 cctcttttaa acctgctttt tgcgccgcgg aatctggcgt aacggcaggt tgcgaacttc
     5161 cgcttgtcac aaacacaccg taatcaaccg gcaggctttc ttcttcctgc aactgcttgg
     5221 ttacgggtac ataccgcaca ccgagaaaag gatactcaat ttccccgcca tttgccattt
     5281 gataaaggtc tcgctgcacc ccctctatgg gaatggcaaa cccaatgctt tgggcttgca
     5341 acacggtagc ggtgtttacc cccactactt cgcccgacaa gttcagcaat ggcccccccg
     5401 aattcccttt gttaatggca gcatcagttt ggataacgtc ttcaatagtt tccaccaaac
     5461 tgcccccgtc agaagccgta atggtacgcc ccaaaccgga aatcacgcca acagaaaccg
     5521 tattctggaa ttctcccagc gcgttcccaa tggcaacaac gctctggcca atttcgaggg
     5581 catctgaatc tcccagcttc acaaacggga aaggttccgc tctctcgatt ttcagaaagg
     5641 ccaaatcctg cagcgggtcg gtagccaaaa ctgtagcgtc atacgaagtt ccatcgcgca
     5701 aaaataccgt atattcgaca ttgtcttgcg agactacgtg ccggttggta gcaaccattc
     5761 catcggcaga aaccaaaaac cctgaaccgc cgccgatttc gcgcctttcc gtgcctcgtt
     5821 gctggagctc gggaatcgaa aagggcgagg gcccaccgaa caaatcttca aaggggtcgc
     5881 gcagcacgcg ctcatatacg ggtacgtctt gtgtgagaat gacgcttacc acggcaggag
     5941 aaacttcctt caccacgtcg ataaccttga gttcttgatc aacctggaca gcagaaccgt
     6001 tgctgtcggt tgaggaaact gctattcctg cttcctcgag ttgctgggtc acttgccagt
     6061 aggcataccc gccagctgcc ccgcccgcca gaaagccaac tacggcagac acgaacacga
     6121 ccccccaaag aggtctctgg attgtggcat tttgaggata atcgggagat tgaaactcca
     6181 ttcttgtttt attatacctt agcttttcgc aaacttgcaa cgctccaagc taataccttt
     6241 attgttttct caatgtcttc caagcgcgtg ggcttgccca aactcaagcg cacggcagaa
     6301 agagcatctc tcgaactcaa ccccagtgct agcagcacat gcgaaggttc ctgtgttttt
     6361 tcagagcacg cagaaccggt agaaacggca atcccttttt ggtcgagcag caccaccacg
     6421 tcgcggccct ctaccccgcg aaaccgaaag tgcgcattgt ttgccaaacg ccgttctgca
     6481 gaaccagtaa gcttgctgtc gggaatcctt cgcaatactc ctttgatg
//
LOCUS       DASRUP010000018         7456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1151350, whole genome shotgun sequence.
ACCESSION   DASRUP010000018 DASRUP010000000
VERSION     DASRUP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151350"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFE94_00840"
     CDS             <1..666
                     /locus_tag="VFE94_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HZX49680.1"
                     /translation="ARAEALAQFGYTPTIWETPHYISDANTYLTAKNSGFTFFTESDT
                     KLFPNQRGYLNKSNGNLWNIPETAFDFPEDAASIAALEPVKKDHILPRLARLNAPYYI
                     FYHNNLDAQLNSLSNIVAKANTYNMWFPGMEEFGNFWLQREQAALATIHDTQLQKISA
                     TVSNSFEGLTLAVRLPDGFHAADVAINGTPSVAAQKTVDGVTFLYPVLPGGSSASADI
                     SYR"
     gene            complement(667..1716)
                     /locus_tag="VFE94_00845"
     CDS             complement(667..1716)
                     /locus_tag="VFE94_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZX49681.1"
                     /translation="MRKFVTPFAAILAMGLFLVGYALYESSSGLGVKSTKLLPPANGI
                     YHGAFPDFGPEEDIVSAKRIQDFEALVGKGIAFAYFSNNWLGGIHFPQKEVQAIASTG
                     ATPFIRMMPRSDWYEGQPEPVYSLQKIADGVFDPELQKWAAEAKETKIPLLVEFGTEV
                     NGDWFSWNARWNGREQGAAVFQQAYRHIINLFREENVTTITWVFHVDAQGEPDEPWNS
                     MAAYYPGDEYIDWIGMSVYGAQEPGDDWQTFQEVFDEAYAEFAAISATKPLALLEFGA
                     HENNKKPAWIAQALESISSGRYPRVKAISYWHSNWENDDGTTSRLRLDSSKESLQAYQ
                     QAIQNPLFVTEPVFE"
     gene            complement(1722..3761)
                     /locus_tag="VFE94_00850"
     CDS             complement(1722..3761)
                     /locus_tag="VFE94_00850"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HZX49682.1"
                     /translation="MIYTCPMHPEVVAREPGKCAKCGMDLVPMGDAEHDEPHSAKASR
                     DAHGGHHAGMEEQFKRRFFAAVPLTLIVLALSPKIQEWFGFEIAFPGINLVLFGLASV
                     IALWAGWPFYQMAKAELQNKAPAMMTLVSFAVLAGYFFSVAATFLFEGESLYWEISTL
                     VLAFLFGHWMEMRAVRGATGALAELAKLIPPTAHKVVDRGTQDVGTGELQVGELVLVR
                     PGEKIPIDGVVKEGESSVNESMVTGESKPVAKKAGSEVIGGTINNDGSLTVEVSKTGE
                     ETAISQIMELIRQAQETKPPVQKLADRAASWLFYIAVLVGSGTFLFWLFVSPQGAIPA
                     IFAGTLAISVVVIACPHALGLAIPTVTTITSSLAAKNGILIRNMKAAEIARKLTYVVF
                     DKTGTLTTGEFAVQEVSSGQILQLAASVEALSEHPIARAIVRKAKENNMQLLRVEDFA
                     RIPGKGVRGKVEGKEILILQAKKEQGRHTIVEIFQNNKKLGAISLADEIRQESFEAIG
                     ALKEMGIKVAMITGDAENVAKSVAKELGIDTYFARVLPEDKVNKIKELQQKGEIVAMV
                     GDGVNDAASLMQAHVGIAIGAGTDVAIESAEIVLVKDNPKDVVKAIKLSQKTNSKMKQ
                     NLAWAAGYNIVAIPMAAGILFPFGILLRPEWSALLMSASSVIVVANALLLKRVKL"
     gene            complement(3766..4440)
                     /locus_tag="VFE94_00855"
     CDS             complement(3766..4440)
                     /locus_tag="VFE94_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZX49683.1"
                     /translation="MKYIIILVLGFVFAVPSMSLAQGMMDIRGDAVDDNHTAREEAEG
                     KAIWEKLQAEEISCNDLSDDNFGALGEYFMGLMTGTSHEAMNAMMIQMMGEEGEEQVH
                     VAMGKRLSGCDTSATFSPQGMSFMPMMNMMGGGWSAPFATNQSFNSMMGFGFTPLGAF
                     GWIFMILWWVLIVVAIVALVRWLMRQSSDTERKGEKTALEILKQRYAKGEIDKQEFEE
                     KKNDIG"
     gene            complement(4463..4675)
                     /locus_tag="VFE94_00860"
     CDS             complement(4463..4675)
                     /locus_tag="VFE94_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49684.1"
                     /translation="MKKSSLMHWTSIVTGIWGALALLAAWVAGEGGTAFGFSQSHLFN
                     DAIVLQLIAISAGVCALYRRQLEKEG"
     gene            complement(4719..5663)
                     /locus_tag="VFE94_00865"
     CDS             complement(4719..5663)
                     /locus_tag="VFE94_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49685.1"
                     /translation="MKNKLIIGLFTGAVAVAVLVFLYSTSDNGIPGISSANVYCSAYG
                     TLTSERPIQSHRSYCIKVSGEASNYDPNAPYVFAYSIVDDEGNVLKEFETAHEKIMHF
                     IVVRKDLANFAHVHPEFNATTGEFTLVDLTFPFDGEYRLFADFTPTTSQIGSDGARLP
                     VTVYHDVEVGNLDNYKPQSLGDSTSIKTVDDYQVALSSDAALVTGWESLLMFTIEQAG
                     QPVTDLEQYLGSLGHAVILREGDFQFIHTHPVDDPMASQTGYAHFMVSFAEAGKYKVF
                     MQFQHQGKIMTSDFIVNVAQGEDSSNEMEGMDMPGMNH"
     gene            complement(5653..5940)
                     /locus_tag="VFE94_00870"
     CDS             complement(5653..5940)
                     /locus_tag="VFE94_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZX49686.1"
                     /translation="MKQDIKKKAIRRLKIVEGQIRGLQRMVQEEKYCIDIINQSSAVK
                     EALSGVEDLMLENHLSTHVVEQMKSGQHAKATHEVMSVYKLSKKRTHHHEK"
     gene            complement(6041..6310)
                     /locus_tag="VFE94_00875"
     CDS             complement(6041..6310)
                     /locus_tag="VFE94_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49687.1"
                     /translation="MVLTKEDLQDIKGVVVEVVEEKVGGIIEKKIPAIVERAIEPYFQ
                     AIQKDFTNVYERFDRIEVLIHNDYKQRIEKLENQVKELRDALAMK"
     gene            complement(6362..6844)
                     /locus_tag="VFE94_00880"
     CDS             complement(6362..6844)
                     /locus_tag="VFE94_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HZX49688.1"
                     /translation="MTIYEKSSLFLLRIGLGWLFLYAGITKVLDPNWSAAGYLNNAKT
                     FPEFYAWLASPPILPFINIVNEWGLTLLGISLLLGVFVRLSSIGGAALMLLYYFPVVE
                     MKAFEFFPTLTVPYIGEHAVLVDEHIVYVLALVALAAFAAGRTWGLDNMVSKNKSWLG
                     "
     gene            complement(6855..>7456)
                     /locus_tag="VFE94_00885"
     CDS             complement(6855..>7456)
                     /locus_tag="VFE94_00885"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HZX49689.1"
                     /translation="KHAIMELGPAGFFFEDYVAAVLREYGYKTTVGTIMKGHCVSHEI
                     DILAEKEGVHHIMELKYHNRRGLKTDVKVAMYSYARFLDVAAAHKKFEATKHKAWVVT
                     NTKFTKSAIKYADCMGIELLGWRYPKQGSLEQLIEQKSLYPITVLPSANRYVREQFAT
                     QKLMFVRDLLHHSAQDLQKKFALNPAISKRLLQEAYSLV"
BASE COUNT         1926 a         1946 c         1549 g         2035 t
ORIGIN      
        1 gcacgggcgg aagccctcgc gcagtttggg tacacgccaa ccatctggga aactccgcac
       61 tatatttcgg acgcaaatac gtacctgacc gcaaaaaact ccgggtttac tttctttacc
      121 gaatctgaca ccaaactgtt cccgaaccag cgcgggtact taaataagtc caatggaaac
      181 ctgtggaata tcccggaaac cgcgtttgac tttcccgagg acgcagccag tattgcagct
      241 ttagagccag ttaaaaaaga ccacattttg ccgcgattag cgcgcttaaa cgctccttac
      301 tacatttttt accacaataa tctggatgcg cagttaaact ccctgtcgaa cattgtggca
      361 aaggcaaaca cgtacaacat gtggttcccc ggaatggaag agtttggcaa tttctggctg
      421 caaagagagc aggctgcgtt agccaccatt cacgatacgc aattgcagaa aatttcagca
      481 acggtttcca attcgtttga gggacttacc ctggccgtac gcttgccaga cgggtttcac
      541 gccgcagatg ttgcaattaa cggaactcca tctgtagcgg cgcaaaaaac agtagatggc
      601 gttacattcc tttacccggt attgccagga ggatcaagcg cgagcgcaga tatctcttac
      661 cgctaattac tcaaataccg gctctgttac aaacaacgga ttttgtattg cttgttgata
      721 cgcttgcagt gattctttag aagagtcaag gcgcaggcgc gaggtagtgc cgtcgtcgtt
      781 ttcccaattg gaatgccaat aagaaatggc ttttacgcga ggataacggc cgcttgagat
      841 gctctccaag gcttgtgcaa tccaggctgg ctttttgttg ttttcatgcg cgccgaattc
      901 caaaagcgcg agcggtttgg tggcggaaat tgcggcaaat tccgcgtatg cttcgtcgaa
      961 cacttcttgg aatgtttgcc agtcgtcgcc cggttcttgc gcgccgtaca cgctcatgcc
     1021 aatccagtca atgtactcgt cgcccggata atacgcggcc atgctgttcc acggctcgtc
     1081 aggttcccct tgcgcatcca catgaaacac ccatgtaatg gtggttacgt tttcttcacg
     1141 gaacaggttt ataatgtggc gatatgcctg ttggaaaact gcggcaccct gttcccttcc
     1201 gttccacctg gcattccacg aaaaccaatc gccgtttacc tcggtgccga attccaccaa
     1261 aagcggaatt ttcgtttctt tggcctctgc tgcccatttt tgcagttcgg ggtcaaacac
     1321 accatccgca atcttttgaa ggctgtacac gggctctggc tgcccctcat accaatctga
     1381 acggggcatc atgcggataa atggcgtggc ccccgtagaa gctatagcct gcacctcttt
     1441 ttgcggaaag tggatacctc caagccagtt gttagaaaaa taggcaaaag caatcccttt
     1501 gcccaccagc gcttcaaaat cctggatgcg tttggcagac acaatatctt cttccggccc
     1561 aaaatcggga aacgcgccgt gatatattcc gtttgccggc ggcagcaatt ttgtgctttt
     1621 cacccctaga cctgacgacg actcgtacaa agcataccca accagaaata accccatagc
     1681 gaggattgca gcaaaaggag taacaaactt tcgcataaaa tttagagttt gacgcgtttg
     1741 agcaaaagag cgtttgctac cacaataacc gaactggcgg acattaacaa ggcagaccat
     1801 tcgggccgca aaagaatgcc gaaagggaaa aggatgccgg ctgccatggg aatggcaaca
     1861 atattgtatc cggcggccca tgccaaattt tgcttcattt tgctgtttgt tttttgtgag
     1921 agttttattg cttttaccac atcttttgga ttgtctttga ccagcacaat ttcggcagac
     1981 tctatggcaa catcggttcc agctccaatg gcaatgccca cgtgggcttg cattaaggaa
     2041 gctgcatcat ttacgccgtc ccctaccatg gccacaattt cgcctttttg ttgcaattct
     2101 tttattttgt ttactttatc ttccggcagt acccttgcga aatacgtgtc aattccaagt
     2161 tcttttgcta cagattttgc cacgttttcc gcgtctcccg taatcattgc tactttaatg
     2221 cccatttcct tcaaggcacc aatagcctcg aacgattctt gtcgtatttc gtctgccagg
     2281 ctaatggcgc caagtttttt gttattctga aatatttcaa cgatggtatg cctgccctgc
     2341 tcttttttcg cttgcaatat tagaatttct tttccttcta cttttcctcg cactcctttt
     2401 ccggggatac gcgcaaaatc ctcaacgcgc agaagttgca tattgttttc ttttgcctta
     2461 cggacaattg cccttgcgat tgggtgttca gacaacgcct ccacggaagc ggcaagttgc
     2521 aaaatttggc cagacgacac ttcttgaacg gcgaattctc ccgtagttaa tgtgccggtt
     2581 ttgtcgaaca ccacgtaggt aagtttgcgc gcgatttccg cggctttcat gttgcgaatt
     2641 aaaataccgt ttttcgcggc caatgagctg gtaatggtgg ttaccgtggg aatagccaag
     2701 cctaacgcgt gcggacacgc gattaccaca acgcttatgg ccaaagttcc ggcaaatatt
     2761 gcgggaatcg ccccttgcgg gcttacaaaa agccaaaaca gaaacgtgcc gcttcccacc
     2821 aaaaccgcaa tataaaagag ccagcttgct gcccggtctg ccaatttttg caccggaggc
     2881 tttgtttcct gcgcctgccg gattaattcc atgatttggg aaatcgccgt ttcctcgcca
     2941 gtcttgctca cttccacggt aagggaacca tcattgttta tggtgccgcc gataacttcg
     3001 cttcccgctt ttttcgcaac cggttttgac tcccctgtta ccatagactc atttaccgaa
     3061 ctctctcctt cctttaccac gccgtctata ggaattttct cgccggggcg aaccaatacc
     3121 agttcgccaa cctgtaattc tccggtacct acatcctgcg tgcccctatc aacaacctta
     3181 tgggctgtag gcggaataag ctttgccagt tccgccaaag ctccggtagc tcccctaacc
     3241 gcgcgcattt ccatccagtg gccaaacaaa aacgcaagca ccaaagtaga aatttcccaa
     3301 tacaaagact cgccttcaaa caaaaatgta gcagccacgg aaaaaaagta gccggcaagc
     3361 acggcaaagc ttaccaaagt catcatggct ggcgccttgt tttgcaactc ggcttttgcc
     3421 atttggtaaa acggccagcc tgcccatagt gcgattaccg aggccaaacc aaaaagaaca
     3481 agattgattc cgggaaatgc aatttcaaag ccaaaccatt cctggatttt aggcgaaagt
     3541 gccagcacaa tcagcgttaa aggcaccgcc gcaaaaaacc gccgcttaaa ctgctcttcc
     3601 atacccgcgt gatggccgcc atgagcgtcc cgcgaagctt tagcggagtg gggttcatca
     3661 tgctctgcat cccccatggg caccaaatcc attccgcact tcgcgcattt ccccggctcc
     3721 cttgccacca cctccgggtg cattggacat gtatagatca tttatttatc caatatcgtt
     3781 tttcttctcc tcaaattctt gtttgtctat ttctcctttg gcgtagcgtt gtttaagaat
     3841 ctccaaagcg gttttttctc cttttctttc cgtatccgag gactgccgca taagccatct
     3901 tacgagagcg acaatggcaa caacaattaa cacccaccaa agaatcataa agatccaccc
     3961 aaacgcgccc aaaggcgtaa atccaaatcc catcatagaa ttaaacgatt gatttgtagc
     4021 gaagggagca gaccaccctc cccccatcat attcatcatg ggcataaaac tcatgccctg
     4081 cggagagaac gttgccgatg tatcacaacc agagaggcgc ttacccattg caacatgaac
     4141 ctgttcttct ccttcctcgc ccatcatttg tatcatcatg gcattcattg cttcatgcga
     4201 ggttcccgtc attaagccca tgaaatactc gccgagagcc ccaaagttgt cgtcggacaa
     4261 atcattacag cttatttctt ctgcctggag tttttcccaa atcgcttttc cttcggcctc
     4321 ctctcttgcg gtatgattat cgtccacggc atcacctcgt atatccatca ttccttgtgc
     4381 caaactcatg cttggcaccg caaacacaaa acctaaaacg agtattatta tgtatttcat
     4441 aaaaaattat gtttgctaat tcttaacctt ctttttccaa ctgccggcga tataacgcac
     4501 aaaccccggc agaaatcgca ataagctgaa gtactatagc gtcgttaaaa aggtgggatt
     4561 gagaaaagcc gaatgcagtc cctccttctc ctgcaaccca tgcggcaagc aaggcaagtg
     4621 cgccccaaat ccctgtaacg atagatgtcc aatgcataag tgatgatttt ttcataacag
     4681 aaagaatgta ttaatgtatt atatataact tcggcctttt agtgattcat accaggcatg
     4741 tccatacctt ccatttcgtt agaagaatcc tcgccttgag ctacatttac tataaagtct
     4801 gaagtcatga tttttccctg gtgctgaaac tgcataaaaa ctttgtattt gcctgcctct
     4861 gcgaatgaaa ccataaagtg cgcgtaccct gtttggctgg ccatcggatc gtccactgga
     4921 tgtgtgtgta taaactgaaa atctccctcg cgaagaataa cggcgtggcc aagagaaccc
     4981 aaatactgct caagatcagt caccggctgg ccagcctgct ctatggtgaa cattagtaaa
     5041 gattcccagc ccgtcacaag agctgcatcc gaagaaagag ctacttgata atcatctaca
     5101 gtttttatgc tggtgctgtc gccaagggat tgtggtttat aattatccaa attgcccacc
     5161 tctacgtcgt ggtatacggt aacgggcagc cttgcgccgt cggaaccaat ttgacttgtt
     5221 gttggcgtaa aatctgcgaa aagccggtat tccccatcaa acggaaaggt aagatctaca
     5281 agcgtaaatt cgccggtcgt agcattaaat tccggatgca catgcgcaaa gttagcaagg
     5341 tcttttcgca cgacaataaa gtgcataatt ttctcgtgcg cggtttcaaa ttcctttagc
     5401 acatttcctt cgtcgtcaac aatggagtac gcaaacacat atggagcatt ggggtcgtaa
     5461 ttgctcgcct cgcccgagac ttttatgcag taactcctat ggctttgtat agggcgctcg
     5521 cttgtgagtg ttccgtaggc cgaacagtat acgttggcac ttgatatgcc cggaataccg
     5581 ttatcgctag tactgtaaag aaatacgaga accgctacag caactgcccc cgtaaatagg
     5641 ccaataataa gtttattttt catggtggtg cgtccttttc tttgataatt tatataccga
     5701 cattacctcg tgtgttgcct tggcgtgctg tcctgatttc atttgctcaa caacatgcgt
     5761 ggaaaggtgg ttctccaaca taaggtcttc cacgccagac aacgcctctt ttacggcaga
     5821 ggattgatta ataatgtcaa tgcagtattt ttcttcttgg accattcttt gcaaaccgcg
     5881 aatttgccct tctacaatct tgagtcttcg gattgccttt ttcttaatat cctgcttcat
     5941 gaatccacta tacccccagg gggtatagag tgtcaagtga aaaaaacgaa aaactaaaaa
     6001 accgcttaaa agcggttccc cgcataacag acaaaccaaa ctatttcatg gcgagggcgt
     6061 cgcgcagctc ctttacctga ttttcgagtt tttcaattct ttgtttgtag tcattatgaa
     6121 ttaaaacctc tatacgatca aaccgttcat aaacattagt aaaatctttt tgaatagctt
     6181 gaaagtaagg ctcgatcgcc ctttcaacta tcgctggaat ttttttttca attatgccgc
     6241 caaccttctc ttcaaccact tccacaacca ctccctttat atcttgtaag tcttcttttg
     6301 ttaaaaccat atttcaatta taaacttcca aatggcactg tcaaatcatc gccacaaatt
     6361 cctatccgag ccaggatttg tttttggaaa ccatgttgtc caaaccccag gtgcgccctg
     6421 ccgcaaaggc tgcgagcgcc actaaagcta acacatacac aatatgttca tctaccaaca
     6481 cggcatgctc gccaatatac gggaccgtaa gtgttggaaa aaactcaaac gctttcattt
     6541 ccaccaccgg gaagtaatac agcagcatta aagcggcgcc tccaattgag cttaaacgca
     6601 caaacactcc caacaaaagc gaaattccca aaagcgtaag cccccactcg tttacaatgt
     6661 taataaaagg aagaattggg ggcgatgcca gccacgcata aaattcagga aacgtttttg
     6721 cgttgtttaa gtacccggcc gccgaccagt tcggatccaa cacttttgtt atgcccgcgt
     6781 acaaaaacag ccaccccaac ccaatccgca ataaaaataa agagcttttt tcgtatattg
     6841 tcatataccc attattatac caaactatat gcctcctgta gaaggcgttt ggatatggcg
     6901 gggtttaggg cgaacttttt ttgcaggtct tgggcagaat ggtggagtaa atcgcgcaca
     6961 aacatgagtt tttgtgtggc gaattgctcg cggacgtacc ggttggccga aggcaacacg
     7021 gttatggggt acaacgactt ttgctcaatt aactgttcca aactcccctg ctttgggtat
     7081 ctccagccca aaagttcaat ccccatgcaa tctgcgtatt ttatggcgct tttggtaaat
     7141 ttggtgttgg tcaccaccca ggccttgtgt tttgtagctt caaatttttt gtgggctgca
     7201 gcaacatcta gaaatctggc gtacgaatac attgccactt taacatcggt ttttaagccg
     7261 cggcggttat ggtattttag ctccattatg tggtgtacgc cctccttttc tgccaaaata
     7321 tcaatttcgt gcgaaacgca atggcccttc ataattgttc ccacggtagt tttgtagcca
     7381 tactcacgca acacggcagc cacgtaatct tcaaagaaaa aaccggcagg ccccagctcc
     7441 ataatggcgt gcttta
//
LOCUS       DASRUP010000019         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1157334, whole genome shotgun sequence.
ACCESSION   DASRUP010000019 DASRUP010000000
VERSION     DASRUP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157334"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1002
                     /locus_tag="VFE94_00890"
     CDS             40..1002
                     /locus_tag="VFE94_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HZX49690.1"
                     /translation="MPFALLGFLLGLPLLLKGADLLVDGGSSLAKRLGISSLVIGLTV
                     VAFGTSMPELLTNIFASLSGATDIAIGNVVGSNIANILLVLGAAAVIFPLSVTKGTVW
                     KEIPFALLAVVVLAFMANDMLLEGKLASEIGFIDGWVLLAFFGVFFAYVLAISKNGNG
                     HAEQNGIQQMSLKKAVIFCVLGIAGLAVGGKFVVDGAVAFAAMFHVSQALIGLTIVAV
                     GTSLPELLTSVVAARKKNSDIAIGNVVGSNIFNVFWILGISAIIKPLPFSPVLNVDIA
                     MVLLATFLLFIVMFIGKRHTLEKWQGAAFLVLYGLYIGYLIFRG"
     gene            1132..1488
                     /locus_tag="VFE94_00895"
     CDS             1132..1488
                     /locus_tag="VFE94_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49691.1"
                     /translation="MPDKRTYADRAEYLKQAVTKRRRKLRAMTVEYKGGKCILCGYKK
                     CHWAFDLHHVNGAEKGFGLSARGLTRSWEKIKQEANKCILVCANCHSEIHAGIVQLPR
                     EILVDKRGENGEALRG"
     gene            2126..3022
                     /locus_tag="VFE94_00900"
     CDS             2126..3022
                     /locus_tag="VFE94_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZX49692.1"
                     /translation="MNWIFLALLAPLFWIFSNYVDKYSLERYVKGIFDFLFFSTLTAW
                     LFTPALVFLFGFPTLTWFSVVPILLGMALIYSYGFYGKALQEGETSRLVILFQLVPVL
                     TLILAFLFFEQTISSKEFTAFFFVLMGAVIISVERFGVRAIFTKGAKWIMVDIVMWSV
                     IFLIADFMLTKMSFTDFLILDTFGTALAGLPLLLIPAMRKQIVYGIKTAVPQKYRWFV
                     LNNFFDFFGQMSLKGALSLSSSAGLVTVIIQVQSFYGIVLGYILTMVLPSIFREDTSK
                     KALGKKIIGALIMFLGVALLLL"
     gene            3227..3412
                     /locus_tag="VFE94_00905"
     CDS             3227..3412
                     /locus_tag="VFE94_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49693.1"
                     /translation="MELWQEQLKNMFTLPNGDAAIEAFDDTKKIVALSPEAHAEIARN
                     IEAVLQSSGINYEVKQL"
BASE COUNT         1025 a          853 c         1020 g         1230 t
ORIGIN      
        1 agcttaaaga aactcaaggg gtactaatag ttagcactta tgccttttgc gttgctcggt
       61 tttttattgg gactgcccct tcttttaaaa ggggctgatt tgctggtgga cggcggttct
      121 tctttggcaa agcgtttggg aatttcttct ttggtaatcg ggcttacggt ggttgctttt
      181 ggcacctcta tgccagagct cttaaccaac atattcgcca gcctttcggg cgccacagac
      241 attgccattg gtaacgtggt ggggtctaac attgcgaata tccttttggt gctgggcgcg
      301 gctgccgtta tatttccgct ttccgtaacc aaaggcacgg tatggaaaga aattcctttt
      361 gccttgcttg ccgtggtggt gcttgccttt atggcaaacg acatgctgtt agaaggcaag
      421 ctggcgtcgg aaataggttt tattgacggc tgggtcctgc tggcgttttt tggcgtgttt
      481 ttcgcatacg tgcttgccat ttccaaaaac ggaaacgggc acgcagagca aaatggcata
      541 cagcaaatga gcctaaaaaa ggccgtaatt ttttgcgtgt tgggcattgc ggggttggct
      601 gttggaggaa agtttgtggt ggacggcgca gtcgcgtttg ccgcaatgtt tcatgtaagc
      661 caggcattaa ttggactgac cattgtggcc gtgggcacaa gcctgcccga gttgcttacg
      721 tcggtggtgg cagcccgcaa gaagaactcg gatattgcca ttggcaacgt ggtgggcagc
      781 aacattttta acgtgttttg gattttggga atttctgcca ttattaagcc tttgccgttt
      841 tctccagtac tcaacgttga tatcgctatg gtgctgcttg ccacgtttct tctgtttatt
      901 gtgatgttta taggcaagcg ccatacgtta gaaaaatggc aaggcgccgc cttccttgtg
      961 ctctacggcc tttatattgg gtaccttatc ttccgcggct agcttgtgct tccgcttgag
     1021 ttttcctcaa aagttggtat actggtagtc gagctccata ttccgcggta gctcagttgg
     1081 tagagcaact cgctgtacgt ttacacacgt ttctggtata ctcaaatgta tatgccagac
     1141 aaaagaacat atgccgaccg cgccgagtat cttaaacagg ccgttacaaa acggagacgt
     1201 aaacttcgcg caatgactgt tgagtacaag ggtggtaagt gtatcctttg tggatacaaa
     1261 aagtgtcact gggcatttga cctacatcat gtaaatggtg cagaaaaggg ctttggcctt
     1321 tctgctcgtg gacttacgcg atcgtgggaa aagataaaac aagaagccaa taaatgtatt
     1381 cttgtttgtg caaattgcca tagtgagata cacgccggca tagtgcagct tccaagagaa
     1441 atcttggttg ataaacgagg tgaaaacggt gaagcccttc gcgggtaata ccgtgccaag
     1501 ctccggcgtt atgccagaga aggtgtagag actatcccga aagggagtac cccgagctag
     1561 ccgaggggga agcgcctcgc accctactct atagatagag gggtgatgat atagtccact
     1621 ccagtatgaa aatattgggt tatgtgtaac gagatggtcg taggttcgaa tcctacccgc
     1681 ggagccgcga aaaatgactt caccccgctt attcaagcgg ggtttgtcat ctcgcggcac
     1741 cgccaagaga tattgaagtc attttgagcg agccacgagg taaatctttg atttactttt
     1801 cgtggcaaca gtactaatct gaaataatgt ctttttatta cgtctacgtt ttacaaaatc
     1861 aaacttcagg aagtctgtat atcggtttta cttccgactt acgaaagcgc gtcgcggggc
     1921 atagttcagg cgatacaaaa acgactaaga gagggaatta tcgacttatc tactacgaag
     1981 cataccgcaa taaaatggat gctttgggca gagagaaatt cctcaagggc ggttccggaa
     2041 gaaaatatct taacaaacaa ttaaaacatt attttaaaga gggttagcaa tcgccatttc
     2101 ggcggttttt tggtagagtt acattatgaa ttggattttc ctagcacttc ttgcgcctct
     2161 tttctggata ttttctaatt atgttgacaa gtacagctta gagcgttatg tcaaaggaat
     2221 ttttgacttt ctttttttct caaccctcac tgcttggtta tttacgcctg cgcttgtttt
     2281 tctttttggt tttccaacct taacctggtt ttctgtcgtc cccatcttgt tgggaatggc
     2341 gttgatttac tcttatggtt tttatggaaa agctcttcaa gaaggggaaa cctcacgtct
     2401 tgtaatactt tttcaactgg ttccagtcct tactcttatc cttgcttttt tattttttga
     2461 acaaaccata tcttccaaag aatttaccgc cttctttttt gttctaatgg gggctgtaat
     2521 tatttctgtt gaaaggtttg gagtcagagc tatttttaca aagggagcaa agtggataat
     2581 ggtagacata gttatgtggt ctgtaatctt tctgattgca gactttatgc ttactaaaat
     2641 gtccttcaca gattttctta tcctggatac ttttggtact gctcttgctg gtcttccttt
     2701 gttattgatt cctgcaatga gaaagcagat tgtttatggt attaaaactg cagttccgca
     2761 aaaatacaga tggtttgtcc ttaataattt ttttgacttc tttggccaga tgagcctaaa
     2821 aggggcatta tctctttcgt catctgcggg acttgtaacc gtaatcattc aggtgcaatc
     2881 tttctatgga attgtactag gatatattct tacaatggtg ctgccaagta tcttcagaga
     2941 ggatacttct aaaaaggcgc ttggtaagaa aattataggt gcgctaatta tgtttcttgg
     3001 cgttgcgctt ttgctgctgt agctataaag gttctaacgt catgcagcgg ggggagctca
     3061 gaatccaagc acacctgaaa gggtgtgttt tggtttctga atttgtgact ggattcgaac
     3121 caacgaattc agggcggttt ttttgtccgt cgtgtccgtc cgcgcggctt gaaaaacacc
     3181 cccaaatggg ttatggtggt ggtatctcgg tctaaaggag aggcaaatgg aactatggca
     3241 agagcagctc aaaaatatgt tcactcttcc gaacggcgac gctgccattg aggctttcga
     3301 cgacacgaag aaaatcgtcg cattaagtcc agaggcgcac gcggagatag cccgaaacat
     3361 tgaagccgtt ctccagagta gcggcattaa ctacgaagtc aaacagctct aggctctagc
     3421 ccggctcttt gaagcaacac tccgccccgg aagtttctaa actcccgggg cgcttttatt
     3481 ggtcgcgggg tagtggagta gtaccgtgtg gtggctgtga atctatgaaa ttccgcaaaa
     3541 aggcggcttg aaaagcgggc ccaaataggc tatggttgta gtaccgaatt tttgaatata
     3601 ctttgcctga aaggaggagg gtatggccac agtaactata gcgcatagca gttttcgaaa
     3661 gcgcatagca gttttcgaag aagttccctc cgctttctgg agacaaaaga tgtcttgcgg
     3721 gtgggaatgt tcattcgctt gcaagataag gaggaaaaat accagggtac ctttcttata
     3781 atcgaaaccg ccccttccac cagctggagc ggccagcatg tgcatcttgt tttattcccc
     3841 ttgggtgtcc agcgagaaac gccaaagctc tatactctcc aaggtaaaaa attagctcgc
     3901 ccataatatc ccggcgagct tttttgcttg attttgggtt agttttgtgt gaaagtgaga
     3961 taacggttaa ccgtagcgca aggagagaaa aatgatctgc cccaagtgcg gcacgccaac
     4021 tgcaaaaaaa gatttaggct ggatgcaccc aagagttccc gagcctcaag atagcggtta
     4081 ctgctatgca tgcgacatga tttattacca atgggtcttg gagggcga
//
LOCUS       DASRUP010000020        10746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1062135, whole genome shotgun sequence.
ACCESSION   DASRUP010000020 DASRUP010000000
VERSION     DASRUP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062135"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VFE94_00910"
     CDS             complement(<1..989)
                     /locus_tag="VFE94_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZX49694.1"
                     /translation="MIKPISISLSPNTEPDDVRLAFGLLFQPDKWKKGSAPQELEQKF
                     AARLGVQHACAFNSGRSAFLAILESLGLEKGSEVLLQAFTCNAVPNPVMWAGLQPVYV
                     DCNESDFNIDVEDLERKITSKSRVVVVQHTFGLPADMSKVMEICQKHNLILIEDCAHA
                     LGAKYKGKEVGSFGKAAFFSFSRDKVISSVYGGMAVTSDDSLASKIQAFQEKAGYPAR
                     YWVWQQLAHPVAMNWKILPTYSVFGKYLLVLSQWLHVLSKAVHWKEKRGMKPGYFPKR
                     MPNAQAILALRQLGKLDRFNAHRKEIAQKYYTALTGSTFELPKQFAERENIFLR"
     gene            complement(986..2461)
                     /gene="murF"
                     /locus_tag="VFE94_00915"
     CDS             complement(986..2461)
                     /gene="murF"
                     /locus_tag="VFE94_00915"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZX49695.1"
                     /translation="MVLAFVIFWLIREVKVMLFWLYLWQLKEYHIGRFLAHFQTAKGR
                     RLLLNPWAGAKLILLLVVLFIPNAIFLLIPLYAVEFFLRKAPKPVFTRKIQFLTLVLL
                     GVSLVFAFLGSLVFLLIIDILTPLVVSLVVLGLQPFTVLARNRVIEQAKAKRRKMKNL
                     KVVGITGSYGKTSTKEFLAHILGQKFTVLKTPEHTNSEVGIANTILRSLTTEHDVFVC
                     EMGAYGKGGIQLLADMAKPDIAIVTGVNEQHLATFGSMENLLSAEGGRELAEALPSDG
                     MAFVNQTVKSKVQYENAKCKVVYYGGAEKVKIEKEKVSFEFEGVKFEVPAYGGHNVQN
                     LLGAIEAARKLGMSLQEIAKAAASMPLGLGALKVKKGTNGATIIDSTYSANPDGVIAD
                     LEYLSLYEGKRVLVMPCLIELGPAAKEAHKRIGQKIAQVCDQAIITTKEHFEDVKEGA
                     GQSDKIVYVENAQEILEKIKDANAILLESRLPESLIKAVEK"
     gene            complement(2443..3222)
                     /locus_tag="VFE94_00920"
     CDS             complement(2443..3222)
                     /locus_tag="VFE94_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX49696.1"
                     /translation="METSQEQTIKLKDISVNYKIAGQGPVILILHGWGSSSDSWFGVQ
                     EILVGKGFCVIVPDLPGFGKTQAPATVWGVGEYAEFIHEFARKLGLEKFVLIGHSFGG
                     QTAIQFAVEHPEKTEKLVLVAAAGVRRTPGVFKKLVAAAAKLVSFLLYLVPSQDLRNN
                     IKHAIYMAIRRRDYVRTQGIMRDVFKLVITQDLTAKFSKISVPTLIIWGDKDELTPVQ
                     DAYLMHELIRNSKLEIIGGGKHALNFQMPDKLSEVIWSWLS"
     gene            3281..3478
                     /locus_tag="VFE94_00925"
     CDS             3281..3478
                     /locus_tag="VFE94_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49697.1"
                     /translation="MPKCQKCNVGQLVPLSDYGGQGAAVHYKAWVCINPTCGFNIKIR
                     NGDIYLNEPITPGSMNIPRSR"
     gene            complement(3501..4739)
                     /gene="serS"
                     /locus_tag="VFE94_00930"
     CDS             complement(3501..4739)
                     /gene="serS"
                     /locus_tag="VFE94_00930"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZX49698.1"
                     /translation="MLDIKLIRENPEAVKEGCKKKGIECDIEQILGLDKRKRELLQEI
                     EGLKARQNKLRKEDAEEGRRLKQEIKALEPQLEEVDGGLQSLLTQLPNLPLEDVPVGK
                     DESANVVLRHVGKVPTFDFEPKDHLQLGEELDIIDVQRAAKVVGTRFGYLKGDAVLLE
                     FALVDLAIKTAVKEGFTPVVPPVFLKSEYMHAMGYVDTPEDLAERYFFEKEGLFLVGT
                     SEQSIGAMHANEVFEEKELPKRYVGFSACFREEAGSHGKDTKGIMRVHQFDKAEFVSF
                     TRPEDSRSEHRLLVELEEKLWQALEIPYQVVQLSTGDMGRPHASTIDIEAWMPGQNRY
                     REVSSASNMTDFQARRLNTRYKTKEGKLEFVHMLNATGFPIGRAIIAILENCQQKDGS
                     IKIPKALQQYLGKKEIKSPS"
     gene            complement(4741..6213)
                     /gene="lysS"
                     /locus_tag="VFE94_00935"
     CDS             complement(4741..6213)
                     /gene="lysS"
                     /locus_tag="VFE94_00935"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZX49699.1"
                     /translation="MAAIDEIKKERIKKLERIQKAGDLAYPASVKRTHTAQEAVAGFA
                     KLSKAETEIILVGRIRAIRGHGGSTFLTLEDGSASIQAYVKKDRVGEQAYDSFLETFD
                     IGDFIQVRGILFTTKKGEKTLEAADYKMLAKSLLPLPDKWHGLQDEEERYRKRYLDLL
                     FNKEVKEKFQKRAQVITAVRDFLDKQGFLEVETPVLQHMYGGADAAPFKTHLNAFDLD
                     MYLRISLELPLKRLIVGGFEKVYEMGRVFRNEGVDRQHNPDFTLLEFYWAYADYKDMM
                     KLIEEFVPAIVRKTTGSNEIIYEGKKISFRAPWPRVEYSELFRKHLKLDIDSLNRDAL
                     ANEAKKLGVEIENAMDKAHITDALYKKGIRPKLWEPQFIIHHPAEMKPLAKVRQEDPT
                     KTESFQLLVGGGWELMNGWSEQNDPAAQKKVFEEQESLFRKGLEDAQRMDVDYIEALE
                     YGMPPTAGVGMGIDRLVALLTDAHSLREIIAFPTMKPKEK"
     gene            complement(6297..7310)
                     /locus_tag="VFE94_00940"
     CDS             complement(6297..7310)
                     /locus_tag="VFE94_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49700.1"
                     /translation="MATTSHDKFEQLRSQLLKKEFQVKDEIRRLLEQEGQIEAEEDQV
                     EQQADQEQNEGTRQDLEKKRWEIEDQRRLVKQERWRIEGNLHAVESQLQKVKRWASLQ
                     EQLESSLNEEADIENQVYDVQEQELREEEPGMKTRLEEKLRELENKEAQLEQKRQELE
                     QKEQGIENPAERREVERERWSMEDELHALEKEQWVVERELQGMRQQIQETEERRWTLE
                     EKRRAVEKEKWEIEQELDQIEQEVPELRSRESAIEAPPQEPVEVPEQPAVQAEEPAAE
                     PPAQEPEPLPEQDSQEEPQEKPRLRIEPQESEQFDLDSGDSRTKVVRTMKGDLEKARK
                     QKK"
     gene            complement(7319..7774)
                     /gene="greA"
                     /locus_tag="VFE94_00945"
     CDS             complement(7319..7774)
                     /gene="greA"
                     /locus_tag="VFE94_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008859960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZX49701.1"
                     /translation="MAKFITKEGLEKLKEDLLYLKKTKQKEVAERLQRAIAFGDLSEN
                     FEYADAKDEQAMLQARIAELEEEIREAKVVVAAEKSDRVQIGSVVEVEAGKSRMTFTI
                     VTGKEANPLEGKISAESPIGEALLQKKVGDKAEVEIPSGMVHYKVISID"
     gene            complement(7779..9725)
                     /gene="mrdA"
                     /locus_tag="VFE94_00950"
     CDS             complement(7779..9725)
                     /gene="mrdA"
                     /locus_tag="VFE94_00950"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZX49702.1"
                     /translation="MSGFPFLKSFKGFQVSREGELELQDVFLDRVIVEREIEENIGFE
                     RKLEIPLSRWLLWGVYAVFLVVMTVFFGRTFYMQVFASDELQAKAEQNAVRSSPLGSD
                     RGVVYDRFGKQLVFNKPSFDFVCDQRDLPEERFERERVLRTAAAVLGIPFEDIKATLE
                     ATKEARVLLAENLSHEHLIQLEARAPELPGCEVQKNTIREYAQNLTLAHLLGYTAKIS
                     SEELRQNPDYFVTDQIGKTGLEKTYEKELRGNPGRVLVEKDALGNVVQEKGEIASEPG
                     NSLSLWLDFELQQKIEESLGATLDRIGAKKGVGIALDPNTGGVLSLVSIPGFDNNLFA
                     RGITRAEYQSITADPLNPLFNRAISGTYPTGSTIKPFVASAALEENVIAPNKEILTHG
                     YIDVPNQYDPEIVYRFLDNANHGAVDMKDAIAVSSNVYFYTIGGGFGDQQGLGPRRIK
                     EYLLRFGWGAQTGVDLSGESKGLVPDPEWKQRVKGEGWWDGDTYNFSIGQGNLLATPL
                     QVANSFVAVANGGTLYKPQMVKEVLDPAGESRQIAPQEIRNGIIEEENLEVVRDGMRQ
                     AVKWGSSVLLNQLPVSSAAKTGTAQTGREDAEGRDYLYSWVTVFAPYENPEIVLTIMV
                     EEAKEGSFAVLPVAKEVLEWYFTR"
     gene            complement(9694..10002)
                     /locus_tag="VFE94_00955"
     CDS             complement(9694..10002)
                     /locus_tag="VFE94_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49703.1"
                     /translation="MMRIIQMCLVLYAFALLQMSFFVHFLPEGIAVNLIFPAVLFIAV
                     FERAESSLSIAAAVFGGLLIDAFSETVFGFWPLILVAIVITVKLILENYVRLPIPKKF
                     "
     gene            complement(10007..>10746)
                     /gene="mreC"
                     /locus_tag="VFE94_00960"
     CDS             complement(10007..>10746)
                     /gene="mreC"
                     /locus_tag="VFE94_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZX49704.1"
                     /translation="GIVVVFFVLALNVFATEARGFMVQVSSPFQAALWDSGNSISTFF
                     GGGALQRDNQRLREENLLLQSQIVSLQDTAEENEELRKVLQFELTGEFPLVMGEIIGK
                     TLADGIITIKAGEEKGLRKGMPVITSGKGVVGRVHEVFQGYSRIQVLSAEESKFEAKV
                     AGKEISGVVAGQGDGKIIFDFVPRDADLAQGDLVVTSQLGNIFPENLLVGEVAEVIKT
                     GAGPFQKAVVKPFFHTKTADVVFVVLL"
BASE COUNT         2549 a         2974 c         2135 g         3088 t
ORIGIN      
        1 cgcaaaaaaa tgttttcgcg ttctgcaaat tgctttggca attcaaacgt gctgccagtt
       61 aaagctgtat aatacttttg cgcgatttct tttcggtggg cgttaaacct atcgagcttt
      121 cctagctggc gcaacgccaa aatcgcctgc gcgtttggca ttcttttggg aaagtaaccg
      181 ggtttcattc cccttttctc tttccaatgc acggctttag acaatacatg caaccactgg
      241 gaaagcacca acaaatactt gccgaatacg ctatatgttg gcaaaatctt ccagttcatt
      301 gccacgggat gcgccaattg ctgccaaacc caatacctcg caggataccc tgctttttct
      361 tgaaatgctt gtatttttga ggccaacgaa tcgtcgctgg taaccgccat tcccccgtac
      421 acggaggaaa ttactttgtc tcgggaaaag ctaaaaaaag cggccttgcc aaaacttcct
      481 acttcttttc ctttatactt tgcgcccaag gcatgcgcgc aatcttctat taaaatcaga
      541 ttatgctttt ggcaaatttc cattacttta ctcatatctg cgggcaagcc gaacgtatgc
      601 tgcacaacta ccaccctact ttttgatgtt atcttgcgct ccaagtcttc aacatcgata
      661 ttaaaatcac tttcgttgca atccacatat acaggctgca accccgccca catgacggga
      721 ttgggaactg cgttgcatgt aaacgcctgc agcaaaacct cgctgccttt ctctaaaccc
      781 aaagactcca aaatcgccaa aaacgcagac cgcccgctgt taaacgcaca cgcatgctgt
      841 acgcccaagc gcgcggcaaa cttttgctcc agttcttgcg gcgcgctccc ctttttccat
      901 ttatctggct gaaacaacaa cccaaacgcc agccgtacgt catctggctc cgtattcggt
      961 gataacgata ttgatatggg tttaatcatt tttctacagc tttaataagc gattctggca
     1021 aacggctttc caataaaata gcattggcgt cttttatttt ttctaaaatc tcttgcgcgt
     1081 tttctacata tactattttg tcgctttggc cggcgccctc cttaacgtct tcaaagtgtt
     1141 ctttcgtagt gataatcgcc tggtcgcaaa cctgtgcgat tttctgccca atgcgcttgt
     1201 gcgcttcttt tgccgctggt cctaactcaa tcaaacacgg cataaccagc actcttttgc
     1261 cttcgtataa actcaaatac tccaagtctg caattacgcc gtcggggttt gcagagtaag
     1321 tcgaatcaat aatagtagca ccatttgttc cctttttcac cttcaaagcg cctaatccca
     1381 gaggcatgct ggctgctgct tttgcaattt cttgcaggct cattccgagc tttcttgccg
     1441 cttcaatcgc ccctaaaaga ttttgcacgt tgtgaccgcc atatgctggc acctcaaact
     1501 ttacgccttc gaactcaaac gaaacttttt ccttctcaat cttcaccttt tccgcccctc
     1561 catagtacac aactttgcat ttcgcattct catactgcac tttgctttta acagtttgat
     1621 ttacaaaagc cataccatct gaaggcagag cttccgctaa ctctctcccc ccttctgccg
     1681 acaataagtt ttccatgctg ccaaacgtag ccaagtgctg ttcgtttacc cctgtaacaa
     1741 ttgctatgtc gggctttgcc atgtctgcca gcaactgtat gccgcccttg ccgtaagccc
     1801 ccatttcgca cacaaaaaca tcgtgctccg tggttaaact ccgcaaaatg gtgtttgcga
     1861 tacccacctc tgaattcgta tgttccgggg tttttaatac ggtaaacttt tggcccaaaa
     1921 tatgcgccaa aaattctttg gtagacgttt tcccatagct tccggtaatg ccgactactt
     1981 tgagattctt catttttctt cttttcgctt ttgcctgttc gataaccctg tttctcgcca
     2041 acaccgtaaa gggctgtaac ccaagcacta ccaaagaaac aaccaaaggg gtgagaatat
     2101 cgattatcaa aagaaacacc aaactaccca aaaacgcgaa gaccaaggaa acacccaaaa
     2161 gcacaagagt taaaaactgt atctttcttg tgaatacagg cttgggtgct tttctcaaaa
     2221 agaattccac cgcatataag ggtatcaaca aaaagattgc attggggata aaaagcacca
     2281 ccaggagcaa aataagcttt gcccccgccc aagggttcaa aaggagccgg cgacccttgg
     2341 cagtttggaa atgggccaga aaccggccga tatggtattc ctttaactgc caaaggtaaa
     2401 gccaaaacag catgacttta acttcccgaa tgagccaaaa aattacgaaa gccaagacca
     2461 tatcacttct gataatttat cgggcatttg aaaattgagg gcgtgtttgc cgcctccgat
     2521 gatttcgagt ttggaattgc gaataagttc gtgcattaag taagcgtcct gcacgggggt
     2581 aagctcatcc ttatcgcccc aaataatgag ggtgggcacg ctaatttttg aaaacttcgc
     2641 cgtaaggtct tgcgtaataa cgagcttaaa tacatctcgc ataatgccct gcgtgcgcac
     2701 gtagtcgcgc ctgcgtatgg ccatatatat tgcgtgcttt atattgttgc gcaggtcttg
     2761 cgacggcacc aaatacaaca aaaaacttac caattttgcc gctgcggcca ccagtttttt
     2821 gaaaactccg ggggtacgtc gcacgccggc tgccgcaacc aatacgagct tctctgtttt
     2881 ttccggatgt tccaccgcaa actggattgc ggtttgcccg ccaaaggaat gtccgatcaa
     2941 aacgaatttc tccaaaccta gctttcgcgc aaactcatgt ataaactccg cgtactcccc
     3001 tactccccat actgtagctg gggcttgcgt cttcccaaat cccggcaaat ccggaacaat
     3061 cacgcaaaac cccttcccta caagtatttc ttgtaccccg aaccacgaat ccgaagaact
     3121 cccccacccg tgcaaaatca aaataactgg gccctgacca gcaattttat agtttacaga
     3181 gatatccttt agtttaattg tttgctcttg actagtttcc ataaaatgct aggttggcct
     3241 tagattacga gaagggagga gtaacatgga agactgggta atgccaaagt gtcagaaatg
     3301 caatgtgggc cagctggtgc ctttgtctga ctacggcggt cagggggcgg cggtccacta
     3361 caaagcctgg gtatgtatca accccacgtg cgggttcaac attaagatcc gcaacggtga
     3421 tatctacctg aatgaaccca ttacccctgg cagcatgaat attccccgat cccgttagga
     3481 ggatggtata gcccagcttt tcagctaggg ctttttattt cttttttgcc aaggtactgc
     3541 tgaagcgctt tgggaatttt tatactgcca tccttttgct ggcaattttc caaaattgca
     3601 ataatagctc ggccaatggg aaaccccgtg gcgtttagca tatgcacaaa ctccagtttg
     3661 ccctcctttg ttttataccg cgtattcaac ctcctggcct gaaaatcggt catgttggaa
     3721 gctgaactta cctcgcggta tctattttgc cccggcatcc acgcttcgat atctatggta
     3781 gaagcgtggg gccggcccat atcgccagtg gaaagttgca caacctggta aggaatttcc
     3841 agcgcctgcc acaatttttc ttcaagttca acaagcagcc tatgctcgct tcgagaatct
     3901 tcgggccgcg taaagctcac aaattccgct ttatcgaact ggtgcacgcg cataatgcct
     3961 ttcgtatctt ttccgtgaga accggcttcc tcgcgaaaac aagcggagaa accaacataa
     4021 cgctttggaa gttctttttc ctcgaacact tcatttgcat gcatggcgcc tatcgactgt
     4081 tctgatgtac ccaccaaaaa caacccttct ttttcaaaaa agtatcgctc tgccaaatct
     4141 tctggcgtgt cgacataccc cattgcgtgc atatattcag attttaagaa cactgggggc
     4201 acaaccggag taaacccttc ttttaccgcg gtctttattg caagatccac caaggcaaac
     4261 tcgagtaaaa cggcgtcgcc ttttaagtat ccaaagcgcg tgcccacaac ttttgccgcg
     4321 cgctgcacgt caataatatc gagctcctcg cctaactgca aatggtcttt tggttcaaag
     4381 tcgaatgtag gaactttgcc gacatgtcgc aacactacgt ttgcagactc gtcttttcct
     4441 acaggcacat cttctaaagg caaattcggc agctgggtaa ggagcgactg caatccccca
     4501 tccacctctt ccagttgcgg ctccagcgct tttatttcct gttttagcct tctcccctcc
     4561 tccgcatcct ctttacgcaa cttattctgc cgcgctttta acccctcaat ctcctgcaac
     4621 aattcccgtt ttcttttatc caaccccaaa atctgctcga tgtcgcactc gatccctttc
     4681 tttttgcatc cttcttttac ggcctctgga ttttctctaa tgagtttaat gtctaacata
     4741 ttatttctct tttggtttca tggttggaaa tgcgataatc tctcgcaggc tgtgggcgtc
     4801 tgtaagcaaa gcaactaaac ggtctattcc catgcccacg ccagcggttg gcggcattcc
     4861 gtactctaag gcttcgatgt aatccacatc catgcgctgg gcatcttcca acccttttcg
     4921 aaataagctt tcttgttctt caaagacttt cttttgcgcc gcaggatcgt tctgttcaga
     4981 ccaaccgttc ataagctccc atccaccgcc taccagtagt tgaaaacttt ccgtttttgt
     5041 gggatcttcc tggcgcacct tggctaaagg cttcatttcg gctggatgat ggattataaa
     5101 ttgaggttcc caaagctttg gacgaatacc tttcttgtaa agggcatcgg taatgtgagc
     5161 cttatccatt gcattttcta tttctacgcc aagtttcttt gcctcgtttg ccaaagcatc
     5221 tcgattaaga gaatcaatgt ctagtttaag atgtttcctg aagagctcgg agtactccac
     5281 gcgaggccaa ggagctctaa acgaaatttt cttcccttca tatattatct cgtttgatcc
     5341 tgttgtctta cggactatag cgggaacaaa ttcctcaata agcttcatca tgtctttgta
     5401 gtctgcgtac gcccagtaaa actcaagcaa agtaaaatca ggattatgct ggcgatctac
     5461 gccttcgtta cggaatactc ttcccatttc gtatactttt tcaaaaccac caactatgag
     5521 gcgttttagt ggaagctcca aggatatacg caaatacata tctaggtcaa acgcgtttaa
     5581 atgtgtttta aatggagcgg catccgctcc cccgtacata tgttggagca cgggcgtttc
     5641 cacctccaaa aaaccttgct tgtccaaaaa atcgcgtact gcggtaataa cttgggccct
     5701 tttttggaat ttttccttta cctctttgtt aaacagcaaa tccaaatagc gcttgcgata
     5761 acgctcttct tcatcttgta aaccgtgcca cttgtctggc aagggcaaga gcgactttgc
     5821 cagcattttg taatctgccg cctcaagtgt tttttcacct tttttggtgg taaacaaaat
     5881 gccgcgaacc tggataaagt cgccaatgtc aaatgtttcc agaaaactat catatgcctg
     5941 ctcccctacc ctgtcttttt ttacatacgc ctgtatgctt gccgaaccat cttccagggt
     6001 aagaaatgtc gagccgccgt gcccgcgtat cgcccgaatt ctgccaacca aaataatttc
     6061 tgtttctgcc tttgaaagtt tggcaaaacc agccacagcc tcctgggcag tatgtgtcct
     6121 tttcaccgaa gccggatacg ccaaatcccc cgccttctgt atccgctcca gcttttttat
     6181 tctctctttt ttaatctcat caatcgccgc catattccct tatattaggc tatttttctc
     6241 ttttggtcaa aagaaaacag ccccaagcgt agtcgagggg gctgttttct tcttttctat
     6301 ttcttttgct ttcgggcttt ttcgaggtcg cctttcatcg tacgaacaac cttagttcgt
     6361 gagtcgccag aatccaaatc gaattgttca gactcttgag gctctattct caagcgcggt
     6421 ttttcctgcg gttcctcctg agaatcttgc tctggtaagg gttccggttc ttgtgctgga
     6481 ggctctgctg ctggttcttc cgcctggacg gccggttgtt ctggtacttc cacaggctct
     6541 tgaggaggcg cttcgattgc gctttcgcga gaacggagtt ctggaacctc ctgttcgatt
     6601 tgatctaact cctgctcaat ttcccatttc tctttttcaa cagcgcggcg tttctcttct
     6661 aaagtccagc gcctttcttc ggtttcctgg atttgctggc gcatgccctg gagttcccgt
     6721 tccaccaccc attgctcttt ttcaagcgcg tgcagctcgt cttccatgct ccagcgctcg
     6781 cgctccactt cgcgccgttc tgcggggttc tcaattccct gctctttttg ttccaactcc
     6841 tggcgcttct gctctaactg cgcctctttg ttttctagct cgcggagctt ttcctccaag
     6901 cgggttttca tgccgggctc ctcttcgcgc aactcctgct cctgcacatc gtacacctga
     6961 ttctcaatgt ctgcttcttc gtttaaagaa gactcgagct gttcttgcaa agaggcccat
     7021 cgtttgacct tctgcagttg cgattccacc gcatggagat ttccttcgat tctccagcgc
     7081 tcttgtttta ccagcctgcg ctgatcttca atttcccaac gctttttttc caggtcctgg
     7141 cgggtgcctt cgttttgctc ctgatctgcc tgctgctcta cctggtcttc ttccgcctcg
     7201 atttggcctt cctgttccaa aagtcttctg atttcgtctt tcacctgaaa ctcctttttg
     7261 aggagttggc ttcgcagctg ttcaaacttg tcgtgagagg ttgttgccat aaaattcctt
     7321 agtcaatact tataacttta taatgcacca ttcccgaagg aatttcaacc tcggctttat
     7381 cccccacttt tttttgcagg agagcctcgc caatcggaga ctcggcggaa attttgcctt
     7441 ccaaagggtt tgcctctttc cccgtaacaa tagtaaaggt cattcgcgat ttgcccgcct
     7501 cgacttccac tacggaacct atttgaacgc ggtctgattt ttctgcggcg accaccactt
     7561 tggcttcccg aatctcttct tcgagctctg cgatgcgggc ttgcagcatg gcctgctcat
     7621 ctttggcatc cgcatattca aaattctccg acaaatcgcc gaacgcgatt gcccgctgca
     7681 atctttctgc tacttctttt tgtttggtct ttttgagata gagcaagtct tctttgagtt
     7741 tttctagccc ctctttggta atgaattttg ccatatcgtt atcgggtaaa ataccattct
     7801 aacacttctt ttgcaacagg cagcacagcg aagctcccct cttttgcctc ttcgaccatt
     7861 atggtcaaca caatctccgg attttcatag ggggcgaata cggttaccca gctatacaaa
     7921 taatctcttc cttccgcatc ttctcttccg gtttgcgcgg ttcctgtttt cgccgcagaa
     7981 gaaaccggca gctggtttaa caataccgac gaaccccatt tcaccgcctg gcgcatgcca
     8041 tcgcgcacta cctcaagatt ctcttcttcg attatgccat tgcgaatctc ttggggcgct
     8101 atttggcgcg attctcccgc ggggtctaaa acttctttta ccatttgcgg tttatacaac
     8161 gtgccgccgt ttgccactgc gacaaaggaa tttgccacct gcaaaggcgt tgccaacaga
     8221 ttcccctggc ctatggaaaa gttataggtg tccccatccc accagccttc gccttttacc
     8281 cgctgtttcc attcggggtc tgggaccaaa cccttgctct cccctgaaag atcaacgcct
     8341 gtttgcgccc cccagccaaa tcgcaaaaga tattccttta tgcgtcttgg acccaaaccc
     8401 tgctgatctc caaacccacc gccaatagtg tagaaatata cgttactgga aaccgcaatg
     8461 gcgtctttca tatccaccgc accgtggttt gcgttgtcta aaaagcgata cacaatttcg
     8521 ggatcgtact gattgggaac gtcgatatac ccgtgggtaa gaatttcttt gttgggagct
     8581 atgacgtttt cttccaatgc ggctgaagct acgaaaggct ttatcgtaga accggttgga
     8641 taggtgcccg aaatcgcgcg gttgaacaaa ggattcaagg gatctgccgt aatgctttgg
     8701 tattctgctc gcgtaatgcc gcgggcaaac agattgttgt caaatccagg aatacttacc
     8761 agcgacagca ctcctccggt attggggtcc aaggcaatgc ctactccctt ttttgcccca
     8821 atacgatcaa gtgttgctcc aagcgactct tcaatctttt gctggagctc aaagtcgagc
     8881 cacaaactca agctgttgcc gggctctgaa gcaatttctc ctttttcctg gaccacgttt
     8941 cccaacgcgt ctttctccac gagcactctc ccggggtttc cccggagttc cttctcgtac
     9001 gttttctcca ggccggtctt gccgatttga tcggtaacaa agtaatctgg attctgacga
     9061 agttcttcgg aggaaatttt tgctgtgtaa cccaacaaat gcgctaaggt aagattttgc
     9121 gcatactccc ggatcgtatt tttctgcacc tcgcatcccg gcaattccgg agccctcgct
     9181 tccagctgaa ttaaatgctc gtgcgaaagg ttttctgcca gcagcacgcg cgcctctttt
     9241 gtcgcctcca aggtagcttt tatatcttcg aaaggaattc ccagtacggc agctgccgta
     9301 cgcaagaccc tttctctctc aaaacgttct tcgggcaggt ctctctggtc gcacacaaag
     9361 tcgaaactcg gcttgttgaa caccaattgt ttcccgaagc ggtcgtatac gacaccgcgg
     9421 tccgatccta aaggagaact ccgcacggca ttttgctcgg cttttgcctg aagctcgtcg
     9481 ctcgcaaaaa cctgcatata aaacgttctc ccaaaaaaca ccgtcatcac caccaaaaaa
     9541 actgcgtata ccccccaaag cagccaccgc gacaagggaa tttccaactt cctttcaaac
     9601 ccaatatttt cttcgatttc cctctcaaca atcactctgt ctaaaaatac atcctgcagc
     9661 tccagctctc cttcccgact tacctggaac cccttaaaac tttttaggaa tgggaagcct
     9721 gacataattt tctaaaatga gttttaccgt gataactatt gcaactagaa tcagcggcca
     9781 aaaaccaaaa accgtttccg aaaaagcgtc gatcagcagc ccgccgaaca cggctgcggc
     9841 aatgctcaag gaagattctg cgcgctcgaa tacggcaata aacagcactg caggaaaaat
     9901 aagatttact gctatgccct cgggaagaaa atgcacaaaa aaactcattt gcaaaagtgc
     9961 aaatgcatac aacaccaaac acatttgaat aattcgcatc atgaatttag agcaatacca
    10021 caaagactac gtctgctgtt tttgtatgga aaaaaggctt tacaaccgcc ttctggaaag
    10081 gacctgctcc cgttttgata acttcggcta cctcgcccac caaaaggttt tctgggaata
    10141 tattgcccaa ctggctcgta acaacaaggt ctccctgggc aaggtcggcg tcgcgaggaa
    10201 cgaagtcgaa aataatctta ccgtcgccct ggccggcgac aacgcccgaa atctctttgc
    10261 ctgctacctt ggcctcgaac ttgctttcct ctgccgacaa aacctgaatg cgagaatagc
    10321 cctggaatac ttcgtgcact ctccctacca cgcccttgcc agaggtaatc accggcattc
    10381 ccttacgaag ccctttttcc tctcctgctt ttatggtgat tataccatct gcaagcgttt
    10441 tgccgattat ctctcccatt acaaggggaa actctccggt aagttcaaac tgcaacacct
    10501 tgcgcaactc ttcgttttct tctgccgtgt cctgcagcga cacgatttgg ctttggagca
    10561 aaagattctc ttcccgcaac ctttgattgt ccctctgcaa cgcccctccc ccaaaaaacg
    10621 tggaaatgct gtttcccgag tcccaaagcg ctgcctgaaa gggcgaagac acctgaacca
    10681 taaacccccg ggcctctgtg gcaaatacat tgagagctaa aacaaaaaag accaccacaa
    10741 tcccga
//
LOCUS       DASRUP010000021         9188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1629552, whole genome shotgun sequence.
ACCESSION   DASRUP010000021 DASRUP010000000
VERSION     DASRUP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629552"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFE94_00965"
     CDS             <1..1365
                     /locus_tag="VFE94_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HZX49705.1"
                     /translation="HFPTRFLETPYVKMSRGVTAPPPMELPTQGLNTVGHVLYRGEER
                     MVSFASREDRRRHFYIVGQTGTGKSSMLREMIRQDIENGEGVGVIDPHGELIEDTLAN
                     IPKERVEDVVLFEPFDLEHPMGLNMLEYDSPEQKDFAVQEMIAIFQKLFPPEIIGPMF
                     EHYMRNAMLALMADPKNPGTLVEIPRMFVDAEYMENRLRQVSDLMVRDFWLKEWKQTT
                     GSTKSDMLGYVVSKIGRFIENEMVRNIIGQSRSVFDLNTIMDEKKIFLANLSKGLTGE
                     INSSLLGLILVSKMQMAAMKRARTSAEARNDFYLYIDEFQNFTTDSIATILSEARKYR
                     LNLIIAHQYMPQLTEQIKNAVLGNVGSIASFRVGAEDAEFLEKHFKPEFSQFDLVNLD
                     NFNLLIKLMVQGKVFAPFRMETIKPKEGNRQLAEPIREVAKLKYGRRKMVVEAEIRER
                     ARIS"
     gene            1378..3618
                     /locus_tag="VFE94_00970"
     CDS             1378..3618
                     /locus_tag="VFE94_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49706.1"
                     /translation="MAKNEKPPTPQEWLESSEGQEFAEQLKRDEQGSRERQANDIEGS
                     EGRKEEDKRKSRKRVEKDEASSRERTEREDRESRRRVREDDAKYGRAVPEEAEPEESN
                     KEKFLAFVEQEITALEQDGTIFPEEAAERRRAAVLVGEQQIENPEEDAFELSRMSMPR
                     LREMAALLTKEYPRPERGHALPGMKPALESPKDEESAKDEEGATSPEAVESEPDSEVE
                     RLARELVEARQKKNRKEKGFQAELKRVANEIDDLGNDVRRQIVDRAKQLEEEPESAET
                     GAESAEDIREKFPMEDLEGTRKEKERDRMDRAFESMRSRFYDESKFRIAEEKLEMAER
                     ILEGRQYDIFAQQGKEYLVANKGVFPELQMLGEGQIRELPKERVMNLLYENEAFKTQL
                     GEHAEWKKAQHLRDSFNLSREHDRDLPSHVKLVAYKELLQSGAPPELLREFWEKIEGV
                     NLQDRIQEKVGSQKAYVDEHRFVRQRELVQEKLASILPKDPKKREAMQTRFEIKASHL
                     RELLGVDVDKATVALLADKGFRIDGIKTKPGGFLWLRKKVEFPRENGTKEKKTMEQAK
                     KYVLWHKEMLETEARNRARFQLKEEWEANMAAEEQRLLQELGAQVPEEVIEQSYTRLQ
                     EDLAKDFERRFVKGEIDDSEKGQEIFEHLRKNKIDAADLIADAKDFQERASFDFEEDE
                     ELLYNMAAKYGFGDRKLWEASRAEIAQKMKEQKEKGGMGVFGFLLSAVFESLVAKEKP
                     AAAQNA"
     gene            3615..3833
                     /locus_tag="VFE94_00975"
     CDS             3615..3833
                     /locus_tag="VFE94_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49707.1"
                     /translation="MKTFITDAQLQKLLSEVGLSQETKQSLLVKIPHMNDFARMELVD
                     LFLNLELLNQEKETVKKWVRQFEKTGKA"
     gene            complement(3877..4258)
                     /gene="rnpB"
                     /locus_tag="VFE94_00980"
     ncRNA           complement(3877..4258)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFE94_00980"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4324..5970
                     /gene="murJ"
                     /locus_tag="VFE94_00985"
     CDS             4324..5970
                     /gene="murJ"
                     /locus_tag="VFE94_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZX49708.1"
                     /translation="MTFSQFFNIQTKTIAFAAGLLVLSSLVSRALGIVRNGLLSWRFG
                     AGETTDIYLAAFRIPDFIYGILIMGGISAVFLPMFSEHFHTSREEGWKFTSNVLNVLF
                     LLLLVLSALAFLFAPFLVEFITPGFTTEQQETTASLTRLMLLSPLLLGVSSVFSGMLH
                     YFGKFLAYSLAPILYNVGIIAGIVFLVPLFGIWGLGLGVVTGALLHSAVQVPAVLHSG
                     FKWSPLFDWRDQAMKKMFLLALPRTIAAASLHVNLVVLTALASLIATGSITIFNYAND
                     VQYFPIGLIGVSFAMAAFPSLSRSFAKKNIKDFQEAFATTFRQIFFFVMPVAILLFLL
                     RAQSVRLVYGAGDKFTWDDTRLTAAALGIFAFGVLFQALIPFLARAFFATKDTKAPTV
                     ISLFSVAFNIVLAVFLIRTLAVPGALGDVLVALLKLEGIEDIRVLALPLALVISGMAQ
                     AILLGIFLAKYVREILQKDLYLSLAKTAVASVALGIVTYGILRLYGGVFFLTTYEEVL
                     GQLLLASMGGILAFAACAFLVKSPEMLYFWKALKGKLYGR"
     gene            5960..7735
                     /gene="lepA"
                     /locus_tag="VFE94_00990"
     CDS             5960..7735
                     /gene="lepA"
                     /locus_tag="VFE94_00990"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZX49709.1"
                     /translation="MAGNIRNFVIIAHVDHGKSTLADRFLELTGAVEKRQMKEQFLDQ
                     MDLEREKGITIKLQPIRLKYGEYVLNLIDTPGHADFGYEVSRSLAAVEGAILLVDATK
                     GVQAQTLANLKLAREQGLAIIPALNKVDLASARIEEVSQSIADLLGITESEVFRISAK
                     TGQNVESLLQAVIAKVPAAKEEAEKPLRALVFDSHFDAFKGVVAYVRVVEGKAKTNDP
                     LFFKATAAKAAAKEVGHFSPKEVPDGSLAAGQIGYIATGLKEPDLVRIGDTITLQQTS
                     HAEPLHGYKPSRPVVFVSFFPQNANDFDSLKDAIWKLKLNDPAFEYEPEAHEALGRGF
                     RCGFLGVLHSEIISERLQREYQVPLVISRPSVEFRLIDTRGREMSVKTAADWPEPHMV
                     QLVQEPWVWLEVVCPTNYFSQVIQLLAQLEGQQLETNNLSANSLSVLYETPLREIVID
                     FYDKLKSATKGMASMDYQISDWRPAHLVKLEIHIAGQKEEAFSQVVSREKAYREGKAI
                     AEKLKDLLPPQQFAVPIQAVVGGDVVARETIRARRRDVTAPLYGGDVTRKRKLLEKQK
                     KGKKELAEKGRVHIPSKVFLDVFRS"
     gene            7812..8129
                     /locus_tag="VFE94_00995"
     CDS             7812..8129
                     /locus_tag="VFE94_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HZX49710.1"
                     /translation="MEKWNTKTTEELAQAISALRSKQEAKKFLRDLLTPQEIMEFSRR
                     WQAARMLSESIPYSQIEKETGLSSTTVARVSRWLNKGMGGYKLMIDRLAKTHHGQTLV
                     RRG"
     gene            8406..8924
                     /locus_tag="VFE94_01000"
     CDS             8406..8924
                     /locus_tag="VFE94_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49711.1"
                     /translation="MRAREIPALVARGNYDMGICGSDWVEESGEEKRLYREAPLSPYV
                     DVVLFCGKDDPLRAPTKVITADSILSEYPSLTQRELKETFGYTLEVVPCSGSAESHVP
                     HDYRFGVCVRETGKTLEESHQRVLCVLYTSGITLIANLDWYQQTLKIPHRLDYESLYG
                     AMERTKAFAQRS"
BASE COUNT         2519 a         1924 c         2499 g         2246 t
ORIGIN      
        1 catttcccca cgagattttt ggaaactccg tatgtaaaaa tgagcagggg agtaacagct
       61 cccccgccca tggaactgcc aacccagggc ctcaacaccg tggggcacgt gctgtatcgg
      121 ggagaagagc gcatggtttc gtttgcttcg agggaagacc ggaggcgcca cttttacatt
      181 gtggggcaga cgggtacggg aaagtccagc atgctgcgtg aaatgatccg tcaggatatt
      241 gaaaacggcg aaggcgttgg cgttatcgac cctcacggcg aacttatcga agacacattg
      301 gcaaatattc ccaaagagcg cgtggaagac gtggtgctct ttgagccttt tgacttggaa
      361 catcccatgg ggttaaacat gttagaatac gacagccccg agcaaaaaga ctttgccgtg
      421 caggaaatga tagccatttt tcaaaagttg tttccgcccg agattatcgg ccccatgttt
      481 gagcactaca tgcgaaacgc catgctggcc ttaatggcag accccaaaaa tccgggcacg
      541 ctggtggaaa ttccgcgcat gttcgtggat gctgaatata tggaaaaccg cttgcgccag
      601 gtttcagacc ttatggtgcg ggatttctgg ctgaaagagt ggaagcaaac caccgggtcc
      661 accaagtctg acatgttagg atacgtggtt tccaaaatcg gccggtttat tgaaaacgaa
      721 atggtgcgca atattattgg gcagtcccgt tcggtgtttg atttaaacac gattatggac
      781 gaaaagaaaa ttttcctggc aaatctgtcc aaagggctta ccggggaaat aaacagctcg
      841 ctgcttgggt taatcctggt gtccaagatg caaatggctg ccatgaaacg ggctcggact
      901 tccgccgaag ctcgcaacga tttttacctg tatatcgacg aattccaaaa ctttaccaca
      961 gacagcattg caaccattct ttctgaagcc agaaagtatc gtttaaacct tattattgcc
     1021 caccagtata tgccccagct taccgagcag attaaaaatg cggtgttggg aaatgtgggg
     1081 agcatagctt cgttccgcgt aggagcagaa gacgccgagt ttttggaaaa gcattttaag
     1141 ccggagtttt cgcagtttga cctggtaaat ttggacaact ttaatttgtt gattaaactt
     1201 atggtacagg gcaaagtgtt tgcgcctttc cgcatggaaa ccataaagcc caaagagggc
     1261 aaccgccagc tggcagaacc cattcgtgaa gtagccaagt taaaatacgg aagaagaaaa
     1321 atggtcgtgg aggcggaaat tagagaacgc gcaagaatat cgtaattttc tattgctatg
     1381 gcaaaaaacg aaaaaccgcc gactccccaa gaatggcttg aaagctccga aggccaggag
     1441 tttgccgaac aacttaagag agacgagcaa gggtcgagag agaggcaggc gaacgatata
     1501 gagggatcag agggaagaaa agaggaagat aaaaggaaat ctcggaaaag ggttgaaaaa
     1561 gatgaggctt cgtcgagaga aagaactgaa cgagaggata gagaatcgag aagacgagta
     1621 agagaggacg acgcaaaata cggcagggcc gtgccagaag aagccgagcc ggaagaaagc
     1681 aataaagaga aatttctggc ttttgtagaa caggagatta cagctttaga gcaagacggc
     1741 accatctttc cagaagaggc cgcagaaaga aggcgggcgg cagttttagt cggtgaacag
     1801 caaattgaaa atccagaaga agacgcgttc gagctttcga gaatgagtat gccgcgctta
     1861 agagagatgg ctgctttgct taccaaagaa tatccaaggc cggaacgagg gcatgcgttg
     1921 cctggcatga aacctgcttt ggagagtcct aaggacgaag aatctgcaaa agacgaggaa
     1981 ggcgcaacca gtccagaagc cgttgagagc gaaccagata gtgaagttga aaggttggca
     2041 cgggagctgg tagaagcacg gcaaaagaaa aatagaaaag aaaaaggctt tcaggctgaa
     2101 ctaaagcgag tagcaaacga aattgacgat ttaggtaacg acgtgcgccg ccaaattgtg
     2161 gatcgagcaa agcagcttga ggaagaacca gaatctgcgg aaacgggggc agagagtgcg
     2221 gaagatataa gggaaaagtt tcccatggaa gatttggagg gtacgcggaa agaaaaggaa
     2281 agggatcgta tggacagggc gttcgaaagc atgcgcagca ggttttacga cgagtcgaag
     2341 tttcgcatag cagaagaaaa gctcgaaatg gcagagcgca tcctcgaggg taggcagtat
     2401 gatatttttg cgcagcaagg caaggaatac cttgtggcca acaaaggagt ttttcccgaa
     2461 ttgcaaatgc ttggagaagg gcaaatccgc gaattaccca aggaaagggt aatgaatttg
     2521 ctttatgaga acgaggcatt caaaactcaa ttgggcgaac acgcagaatg gaaaaaagcg
     2581 cagcacttgc gcgattcgtt taacttatcg agagaacacg acagagatct gccttcgcac
     2641 gtaaaactag tggcgtataa agagttgttg caaagtgggg ctccgccgga gcttttgcgc
     2701 gagttttggg aaaaaatcga aggagtaaat ctgcaagacc gcattcagga aaaagtgggc
     2761 agccaaaagg cgtatgtgga cgaacaccgg tttgtgaggc agcgggagtt agttcaagaa
     2821 aagctcgcca gcattcttcc caaagatccc aaaaaacgcg aggccatgca gacaaggttc
     2881 gagataaaag cctcccatct tcgcgagctc ttaggcgttg atgttgataa ggcaacagta
     2941 gcactgcttg ccgataaggg gtttcgtata gacggtatta aaacaaaacc aggaggattt
     3001 ttgtggctac gcaaaaaagt ggaattccct agggaaaacg gcaccaaaga aaagaagacc
     3061 atggagcagg caaagaaata cgttctttgg cataaagaaa tgttagagac ggaagccaga
     3121 aaccgagctc ggttccagct caaggaagaa tgggaagcaa acatggcggc cgaagaacaa
     3181 aggcttttgc aagaattggg tgcccaggtc ccagaagaag taatagagca aagctatact
     3241 cggctgcagg aagatttagc aaaagatttt gaaagaaggt ttgtgaaagg ggaaattgat
     3301 gactctgaaa aggggcagga gatatttgaa cacttgagaa aaaacaagat agacgccgca
     3361 gaccttattg cagatgcaaa ggattttcaa gaacgcgcaa gttttgactt tgaagaagac
     3421 gaagaactac tgtacaacat ggccgcaaaa tatgggtttg gcgatagaaa gttgtgggag
     3481 gcttcgagag cggaaatcgc tcaaaaaatg aaagagcaaa aagaaaaagg gggtatgggt
     3541 gtgttcggtt ttttgctttc cgcggtattt gaatctttgg tagcaaaaga aaaaccagct
     3601 gcagcacaaa acgcatgaaa acgtttataa cagacgcaca actgcaaaag ttactaagcg
     3661 aagttgggtt gagccaagaa actaaacagt cgctgttggt aaaaattccc catatgaacg
     3721 attttgcgcg catggaattg gttgatttgt ttttgaattt ggagctgtta aatcaagaaa
     3781 aggagacggt aaaaaagtgg gtaaggcagt ttgaaaaaac cggcaaggcg taagagcctt
     3841 acaaacaaaa aatccgccga cgaggcgggt ttttcgtgtt ggaggaagcc tataagccga
     3901 attctgtatc ccgccagtcc atggaccggc gaaacgacag tcatctatct agctcctcga
     3961 ttgctctcgg agtcatgcga gctacttttt cacagaagtt ctacatgaat tgcagtcctt
     4021 ctgttttgct cttgcgctcg ggtaaggatt tggccgtttc actcttgcat tgctgcaaag
     4081 ttatccctcg attgctctcg ggacgctcgg cctttcggcc tcgccgtcac tgttcgcacc
     4141 tcttggtttg caccagacgg gcgttacccg ctaccctgct actttctagt cttctagaac
     4201 gtgagtgttc ggactttcct ctccgtgttt aaggcggagc gactgtccag cttcctccaa
     4261 cacgcttatt gtagcaaggc tttccgcatt tgtcaagcat taaaatttgc gtataataga
     4321 gagatgacat tcagtcagtt ttttaatatt caaaccaaaa ccattgcctt tgccgcaggc
     4381 cttttggtgc tttcctcgtt ggtaagcagg gccttgggca ttgtgcgaaa cgggcttttg
     4441 tcgtggcgtt tcggggcagg ggagactacc gacatatacc ttgccgcttt ccgcattccc
     4501 gattttatct acggcatttt aattatgggc ggaatttctg ccgtgttttt gcccatgttt
     4561 tccgaacact tccatacaag cagggaagag gggtggaaat ttaccagcaa cgtgctgaac
     4621 gttttgttcc ttttgctctt ggtactttcg gctctggcgt ttctttttgc gccctttttg
     4681 gttgaattta taacgccggg tttcaccaca gaacaacagg aaactacagc cagtcttacc
     4741 aggctcatgc ttttgagccc gctcttgctc ggagtttctt cggtgttttc cgggatgctg
     4801 cactactttg gaaagtttct cgcttactcg cttgctccca ttttgtacaa tgtgggcatt
     4861 atcgcgggga ttgtgttttt ggtgccgctc tttggcattt gggggttagg cttgggcgta
     4921 gtaacgggag ctttgctgca ttctgcggtg caggtgccgg ccgtgctgca ttctggcttt
     4981 aagtggagcc ccttgtttga ctggcgcgac caggccatga aaaaaatgtt tttgctggcc
     5041 ttgccccgaa cgattgccgc agccagtttg catgtaaacc tggttgttct tacggccctg
     5101 gcgtctttga tagcgacagg aagcattact atttttaact acgcaaacga tgtgcagtat
     5161 tttcctatcg gtttaatcgg ggtttctttc gcaatggcag cgtttccttc gctttcgcgt
     5221 tcttttgcaa agaagaacat aaaagatttt caggaagcct ttgccaccac gttccgtcag
     5281 attttctttt ttgtaatgcc ggtggcgata ctattgtttt tattgcgggc gcagagcgtg
     5341 cggctggtgt atggagccgg cgacaaattc acctgggacg atacaaggct taccgcggcc
     5401 gctcttggca tttttgcctt tggcgtgttg ttccaggccc tcattccctt tttggcgcgc
     5461 gctttttttg ccacaaaaga caccaaagcc cccaccgtca tcagtttgtt ttcggtggcg
     5521 tttaatattg tgctggcggt gttccttata cgaacacttg ccgtccctgg cgcgctgggc
     5581 gacgtgttgg tggcgctttt gaaactcgag ggaatcgagg atattcgcgt gcttgccctg
     5641 cctttggcgt tggtgatttc gggcatggcg caggccatac tgctgggcat atttctggca
     5701 aaatatgtgc gggaaatttt gcaaaaagat ttgtatcttt ccttggccaa aacagcagtt
     5761 gcttctgttg cattggggat tgttacgtat ggaattttgc gtctctacgg gggcgtgttt
     5821 ttcttaacga cgtatgaaga agtcttgggc cagttactat tagcttctat ggggggcata
     5881 ctggcttttg ctgcctgcgc ctttcttgta aagtcgcccg aaatgttgta tttctggaaa
     5941 gcgcttaaag gaaaacttta tggcaggtaa tattcgaaat ttcgttataa tcgcgcatgt
     6001 ggatcatgga aagtcgactt tggccgaccg ctttttggaa ttgaccggcg ccgtagaaaa
     6061 gcggcaaatg aaagagcagt tcttagacca gatggacctg gagcgggaaa aggggattac
     6121 cataaagtta cagcctattc ggttaaagta cggcgaatac gttttgaatc tcatcgacac
     6181 tcccgggcat gcggattttg gctacgaggt ttcgcgctct ttggctgcgg tagagggcgc
     6241 gattttgctg gtggacgcca ccaagggcgt gcaggcgcaa accctggcta atttgaaact
     6301 ggcaagagaa caggggctgg caattattcc cgctttgaat aaagttgatt tagcttcggc
     6361 gagaatcgaa gaagtttccc aatcgattgc cgatctgctt ggcattacag agagcgaggt
     6421 tttccgcatt tcggcaaaaa ccgggcaaaa cgtggagagt ttgctgcagg cggtaatcgc
     6481 aaaagtccct gcggcaaaag aagaagccga aaagcctttg cgcgccctgg tgttcgattc
     6541 gcacttcgac gcttttaaag gtgtggtggc gtatgtacgc gtagtagagg gaaaagcaaa
     6601 aacaaacgac cctttgtttt tcaaggcaac cgcggcgaaa gctgcggcaa aagaggtagg
     6661 gcatttttcg cccaaagaag tgccagacgg cagcctggcg gcaggacaaa ttgggtatat
     6721 tgccaccggc ctcaaagagc cagacttagt gcgtattggc gacacgatca cattgcagca
     6781 gacttcgcac gcagaaccgc tgcatggcta caagccttcg cgtccagtgg tgtttgtaag
     6841 ctttttcccg caaaatgcca atgattttga ttctcttaaa gacgccatat ggaaattaaa
     6901 actcaacgat cctgcattcg agtatgaacc ggaggcacac gaggctttgg gcaggggatt
     6961 caggtgcgga tttttgggag tgctgcactc ggaaattatt tcagagcgct tgcagcgaga
     7021 gtaccaggtg cccttggtaa tttcgcgccc gtctgtggag tttcgcctta tagatacgag
     7081 gggccgcgaa atgtcggtga agaccgcggc agactggccc gagccgcata tggttcagtt
     7141 ggtccaggaa ccctgggtgt ggcttgaggt ggtttgcccg acaaactatt tttcacaggt
     7201 aatccagttg ttggcgcaat tggaaggcca gcaactggaa accaataacc tttctgcaaa
     7261 ctcgctttcg gtgttgtacg aaacaccgct gcgggaaatt gtcatagatt tttacgacaa
     7321 gcttaaaagc gccaccaaag gcatggcttc tatggattac cagatttcag actggaggcc
     7381 ggctcactta gtaaagctgg aaattcacat tgcgggccaa aaagaagaag cgttttcgca
     7441 ggtggtttca cgcgagaaag cgtatcgcga aggaaaagcg attgcagaaa aattaaaaga
     7501 cctcctgccg ccccagcagt ttgccgtgcc cattcaggcg gtagtggggg gagatgttgt
     7561 tgcaagggaa accataaggg caaggcggcg cgacgttacg gctcccttgt atgggggcga
     7621 cgttaccaga aaacgcaagc tgctggaaaa gcagaaaaaa ggcaaaaaag aactcgccga
     7681 aaaagggcga gtgcacatac cttccaaggt attccttgat gtcttccgca gctaggggat
     7741 attgacaggg gtctttggcc ttgctatact ttagctaact aaagtgcttt agtataataa
     7801 agcagaaaag tatggaaaaa tggaatacca aaacaactga agaattggcc caggcgattt
     7861 ctgccttgcg cagcaaacaa gaagcaaaaa agtttttgcg tgatttgctg acgccccaag
     7921 agattatgga atttagcagg cgctggcagg cagcccgcat gttaagcgaa agtataccct
     7981 attcgcaaat cgaaaaagaa acgggtttaa gctcaaccac ggtagctcgt gtttcccgct
     8041 ggctcaataa aggcatgggt ggatacaagc ttatgataga ccgccttgca aaaacgcatc
     8101 atggtcaaac ccttgtgaga aggggatgat tgtgattctt tgacgtttat tttagtcaca
     8161 ctcgctccct tctcaaaagg gagttttctt ttgagaagag ttgatcttta acaaaataag
     8221 gaggaagtag gatgttacga attgcattcc caaaaggacg tgatttgact ctggcgaggg
     8281 gacatgtgga cgatctcttg agagatgttt ttggcggacc tctttgggtg aagagaaggg
     8341 caaaatatat atgggagtgg gcagagaggc caaatgaaga gtccacagtt tgtcgcggcg
     8401 tgtttatgcg ggcgcgggaa attccagcac ttgttgcgag aggaaattac gacatgggga
     8461 tttgtggatc cgattgggta gaggagagcg gagaggaaaa aaggctttac agggaagcgc
     8521 ccctttctcc ctatgttgat gttgtactgt tttgcggtaa agacgatccg cttcgagctc
     8581 ccacgaaggt aattactgct gactcaattc tcagtgaata ccctagcctt acacagagag
     8641 aactgaaaga aacttttggt tatactctgg aagttgtacc ttgttctggt tccgcagaat
     8701 cgcacgttcc acacgactac cgttttggcg tatgtgtgag ggaaacagga aaaactctcg
     8761 aagaaagtca tcagcgtgta ctctgtgtac tttacacatc gggtataacg ctgattgcga
     8821 acctcgattg gtatcaacaa actctcaaga ttccgcatcg gttggattat gagtctcttt
     8881 acggggcaat ggaacgcaca aaggcttttg cgcagcgtag ctaaaatccg cagctcttta
     8941 aaggggcagt ttcgtatgga cgagctgccc cttcactttt tgagggagaa tggctataat
     9001 gcctgtactg ggtgggttag aataagccgg tgccgaatag gggggctaag agaaagccga
     9061 acatagagag cacgatgagc aggacaacga agatccagat tccgcgggca atttgttttg
     9121 attttagttt catttcaaaa tttcactttc cttcgtttcc actatggtag caaaagtttc
     9181 ccaatttg
//
LOCUS       DASRUP010000022         7416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_381727, whole genome shotgun sequence.
ACCESSION   DASRUP010000022 DASRUP010000000
VERSION     DASRUP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381727"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..2076)
                     /gene="topA"
                     /locus_tag="VFE94_01005"
     CDS             complement(31..2076)
                     /gene="topA"
                     /locus_tag="VFE94_01005"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZX49712.1"
                     /translation="MKKLVIVESPTKAKTIQQFLGKDYVVQSSFGHVRDLPKSKLGVD
                     VDKNFEPSYVIPTKARKTVSLLKKEAKKADSVILASDEDREGESIAWHLAQALGLQDP
                     ERIVFHEITKQAIQEALEHPRKIDNNLVEAQQGRRILDRLVGYKLSPFLWKKVARRLS
                     AGRVQSVAVRLVVDREREIEKFKPEEYWSVEALFEKEKKQFKAALSAKDGKAIEKLGI
                     KNKETADTILKDLEGAEYKVAQIEKKETKKNPLPPFTTSTLQRAAASRFHFPARLTMS
                     VAQQLYEQGHITYHRTDSLNLAQSALAAAQEFITKEYGQNYWTGAPRRFKTKSKGAQE
                     AHEAIRPTVPSRQPGSVKLTPQQTKLYKLIWQRFIASQMAPAVFDSTSIDIQAINNQQ
                     STIPVYTFRATGQMQKFEGFLKVYPMKFEETDLPNLEKDEILDLKKLLSEQHFTQPPP
                     RFNEASLIKALESYGIGRPSTYAPILSTIQDRNYVEKDDQRRLRPTEIGLAVNDLLVA
                     HFPQIVDVAFTAQMEEELDEIAEGKKEWQPMLKEFYGPFAANLAKKEKEVAEQKITAE
                     ETDKQCPECGKPLLIRFGRYGKFYACSGFPECKYTASLEENNINVPCPKCGKGQLTAK
                     RTKRKKIFYGCNRYPECDFALWDKPTGEKCQTCNSLMVEKAKAAKCSNKDCETNKKK"
     gene            2216..2983
                     /locus_tag="VFE94_01010"
     CDS             2216..2983
                     /locus_tag="VFE94_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HZX49713.1"
                     /translation="MRYIFVDESRISKGRYQLFGSLWLPREKRDEFERTFWQLWDREF
                     PTRRSELKWTKVSKGKLETYQRFIDFFSEFPMVDFRCVVLDTHAIDYQVYHNNDAELG
                     FYKFLYFFLSRNIEKDYIHRGVRESYQIFVDRRRKENGIEVGRLEDLKRVLKNRLDDT
                     CAEVLSPCVRNVEAVDSKESPEVQIVDMLMGAVGYAWEGFETSPAKLIIVEHIEQMFG
                     LKLNRQTPYLSEKINIWKFRLQDKKKSAPRPTPPERG"
     gene            3212..4627
                     /locus_tag="VFE94_01015"
     CDS             3212..4627
                     /locus_tag="VFE94_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49714.1"
                     /translation="MNFFQDFRTETKLLLAVALAAAVFVVGAILLLQNFSQPDETVYS
                     GSCAWRPDQQDYGPCEVFWGSYFDGEKCIGIGGCGPRPNVPFSLMEACNSACVSIPPQ
                     AKRVFPTQEECEAQTGYRCDFVTCDINCPEGFQKGWSATEVKPQQIDTSGWQTYRNDE
                     FGFEVKYPLDWESIQGDQSGLRNQTDYAYISIDSVPIKTGQEMLDVVIANTGMGECSP
                     PDCRHPGEDEVFMRNFDNHLFYYVFTNLFEGRLGVAYYIENPEGGTAIRFGFSAYTGS
                     QNWPLRGEHTDEEEPDHDILKQILSTFRFMEEEKSIQEIDFASYFSSIPPYSDCDEVA
                     PSLSAVFVNNIEYFDVTDDGREEAIVIASSCYTHTAGPDIVGVYQANLDGEIQPLEFK
                     ESLQSHLIRWNPNKQVLIGFTGAYEAGDRNCCPSKEKIVEYQWDNNIQRFRILSEETR
                     ERVHRNKAFWDDDCYIDTVAC"
     gene            complement(4647..6632)
                     /locus_tag="VFE94_01020"
     CDS             complement(4647..6632)
                     /locus_tag="VFE94_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX49715.1"
                     /translation="MDLSNLPADRQAQKESILITSITPYFLEKGRLWFKENLKKILEA
                     AKTQQFFADNILFLEKDRQYSVSELLRKLDELGYEKVLEVRDMGEFSHQGGMVEFFPI
                     NSSNAIRVEFVGNRVDTIEQLPIFVTNEEQAKEILQKRLKSQRLFSDLKNLKENEYLV
                     HLDHGVAKFTGFEEIGKEKYYVLEYAAEDKLFVPVGLERKLSRYVGFADPHIARLGSP
                     TWQKTKRKAKEEIERLAKELLEIYAKREVSSRPAYEEAQDIEQKLKETFPYEETPDQI
                     QALQDIYEDLAQEEPMDRIVCGDVGFGKTEVALRAMVRAAHNGWQAALLAPTTILVDQ
                     HYKNFEARLKDLPIRLAALSRLQSRKEQEKVLQELKNGNIDIVVGTHRLLSNDVEFKN
                     LGLLVLDDEQRFGVKQKEKFKEMRSSLDVLSLSATPIPRTLYMALSSLKKISTIQTPP
                     EGRLPIQTTVAKRNEKIITEAIQKELARGGQTYYLHNRVGTIEQVKKHLERLVPSATF
                     GVAHGRMREDQLIKVIEDFRAKRFDVLIATTIIENGLDMANVSTIIAEDATKLGLSQA
                     YQIKGRVGRSNIQSYAYFLYGGRLTPKANLRLKALKEAEELGSGYKIAMKDLEIRGAG
                     NILGREQAGTVNTIGLNLYCQMLSESVERMKTARPAP"
     gene            6703..7398
                     /locus_tag="VFE94_01025"
     CDS             6703..7398
                     /locus_tag="VFE94_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX49716.1"
                     /translation="MKAVFIGGDGRTCEIATLAMRLRWPDVSPVTMPTAVEGLETVER
                     ESPDVVLLHPDFDDLTLAKAIQELRGFTNVPLLVLGRKGDEMELVTSLELGADDYIRL
                     PCDLTEIMIRIWALLRRAGGESEGPLRSGHLFVNPATYKVFLGECRVFLTPAEFLLLH
                     LLMKNRGIVMPLQTLERALRHEYVDVNSGLVKKYVQRLRRKLGDNAWEPLWIDNVYGV
                     GYRFVGPPPEKPT"
BASE COUNT         1815 a         1604 c         1794 g         2203 t
ORIGIN      
        1 tattttacca gagaatttgg ggtttgtaaa ctacttcttt ttgttggttt cgcagtcttt
       61 gttggagcat ttggcggctt tggctttttc taccataagc gagttgcagg tttggcattt
      121 ctcgcccgtg ggcttgtccc acaaggcaaa gtcgcattca gggtatcggt tgcagccgta
      181 aaatattttt ttgcgtttgg tgcgtttggc ggtaagctgg cctttgccgc atttggggca
      241 ggggacgttg atattgtttt cttccagcga ggctgtgtat ttgcattcgg gaaagccaga
      301 gcacgcgtaa aacttgccgt acctgccaaa gcgaataagc aggggttttc cgcactccgg
      361 gcattgtttg tcggtttctt ctgccgttat tttttgttcg gcaacttctt tttctttttt
      421 tgctagattt gcggcaaacg ggccgtaaaa ctctttcagc atgggctgcc attctttttt
      481 gccttcggca atttcgtcta gctcctcttc catttgcgcc gtaaaggcaa cgtccacaat
      541 ttggggaaag tgcgcgacaa gcaaatcgtt tacggcaagc ccgatttcgg tgggacgcag
      601 gcggcgctgg tcatcttttt ccacgtagtt gcggtcttga atagtagaaa gaataggggc
      661 gtaggtagaa ggcctgccaa tgccataact ttccaaagcc ttaattaaac ttgcttcgtt
      721 aaagcgcgga ggcggctggg taaagtgttg ttccgataaa agctttttta agtcgagtat
      781 ttcgtctttt tccaaattgg gaaggtcggt ttcctcgaat ttcatggggt atacttttaa
      841 aaaaccttcg aacttttgca tttggcctgt cgcgcgaaat gtataaacag ggattgttga
      901 ttgttgattg ttgattgctt gtatgtctat ggaagttgaa tcgaatacgg ccggcgccat
      961 ttgcgaggcg ataaaccttt gccaaataag tttgtacagc tttgtttgct ggggcgttaa
     1021 cttgacgctg cccggttggc gagagggaac agtgggcctg attgcttcgt gcgcttcttg
     1081 ggcgcctttt gattttgttt tgaaacggcg aggagcccct gtccaatagt tttggccgta
     1141 ttctttggta ataaattctt gtgccgcggc aagcgccgat tgggcaaggt ttaacgaatc
     1201 tgtccggtgg taggtaatgt gcccctgttc atacaactgc tgggcaacac tcatggtaag
     1261 ccgggcagga aagtgaaagc gtgaagccgc ggcgcgctgc aacgtagaag tggtaaacgg
     1321 cggcaaaggg tttttcttgg tttctttttt ctctatttgg gctaccttat attctgcccc
     1381 ttccaagtct tttaaaatag tatcggctgt ttccttgttt ttaattccga gtttttcaat
     1441 ggctttgccg tcttttgcag agagcgctgc tttaaactgt ttcttttctt tttcgaacaa
     1501 tgcttcaacg ctccagtatt cttctggttt aaacttttct atttcgcgct ccctatctac
     1561 caccaaacgc acggctaccg attgcacgcg gccggcagac aacctgcgcg ccaccttttt
     1621 ccaaaggaaa ggggagagtt tgtagccaac cagccggtct aaaatcctcc tgccttgctg
     1681 ggcttccacc aaattgttat caattttgcg ggggtgttcc aacgcttctt gaatagcttg
     1741 tttggtaatt tcatggaaaa caattcgctc tgggtcttgc aaacccaggg cttgggccag
     1801 gtgccaggca atgctttcgc cttcgcggtc ttcatcggaa gcaaggatta cggagtctgc
     1861 cttttttgct tcttttttta gtaaggaaac cgtttttctg gcttttgtgg gaattacgta
     1921 gcttggctca aagtttttgt ctacatcaac ccccagcttg cttttgggta aatcccgcac
     1981 atggccaaaa gaagactgca ccacgtagtc ttttcccaaa aattgctgaa ttgtttttgc
     2041 tttggttggg ctttcaacaa taaccaattt tttcatacaa gtttggagta tactcgaata
     2101 taaaaatttt gcaagtgtat caataaaact gttcttactc tagcatatac tttccagaaa
     2161 acaaaaattc accccctatt gattttaagt aagggaaact ggtaaagttg agggtatgcg
     2221 atatattttt gtagatgaga gtcggatttc aaaggggcga tatcagctgt ttggcagtct
     2281 atggttacca agggaaaaac gagacgaatt tgaaagaacg ttttggcagc tatgggacag
     2341 agagtttccc acgcgaagga gcgagctcaa gtggacgaaa gtgtcgaaag gaaagctgga
     2401 aacgtaccag cggtttattg atttcttttc tgaatttcct atggttgact ttcgctgcgt
     2461 ggttctggat actcatgcaa ttgactacca agtttatcac aacaatgacg cagagcttgg
     2521 tttctacaag tttttgtatt tctttttaag tcgcaatata gagaaagatt atatacaccg
     2581 cggagttagg gaatcttacc agatttttgt cgataggcga agaaaggaga acggcataga
     2641 ggttgggcgt ttggaggatt taaagagagt ccttaagaat aggttagatg atacatgtgc
     2701 tgaagttttg tcgccttgcg tacgaaacgt agaggcggtt gactcaaaag aatcgccaga
     2761 agtgcaaatt gtggatatgc ttatgggtgc cgtaggatat gcgtgggaag gctttgagac
     2821 aagtccggca aagttgataa ttgttgagca tatcgaacag atgtttggct taaagctgaa
     2881 caggcaaact ccgtatcttt cagagaagat aaatatttgg aaattccgtt tgcaagacaa
     2941 aaagaaaagc gccccacgtc ctactccccc tgaaaggggc taggtgtacg ctaggtacac
     3001 cgttcggcgc atggtggcac tcttctttat taacactgta acatagaaaa aggtgtcaat
     3061 caagagtttt ccacaattag ctttttgtgg gtttttgtgt gcatacgtct gtagtataat
     3121 gaagcaatga atcccgttag aaatgtcgct ggtcaaacgg catctatcgg ggcaataaag
     3181 attaggtaac aaaaagagca tttctaacgg gatgaatttc ttccaagact tccgaaccga
     3241 aacaaaactt ttgctcgccg tggctctcgc cgccgccgtt tttgtcgtcg gcgcgatttt
     3301 gctgctgcag aacttttccc aaccagacga aacagtatat tcgggtagtt gtgcgtggag
     3361 gcccgatcaa caggattatg gcccatgtga ggtattttgg ggatcgtatt ttgacggaga
     3421 aaaatgtatt gggatcggtg gatgtggacc acgtcccaac gtaccttttt ccttgatgga
     3481 agcttgcaat tcagcatgtg tttcgattcc cccacaggcc aaaagagtat tcccaactca
     3541 agaagaatgt gaagcgcaaa ccggttatcg ttgcgatttt gtaacttgtg atatcaactg
     3601 tccagaagga tttcaaaagg gctggtctgc aaccgaggtg aaaccccaac aaatcgacac
     3661 ctccgggtgg caaacgtacc gcaacgatga gtttgggttt gaggtgaagt atcctttaga
     3721 ttgggaatca atacaaggtg atcagtctgg tttacggaat caaacggact acgcatatat
     3781 ttctattgat agtgttccga taaaaacagg acaagagatg ctagatgttg ttatcgcaaa
     3841 tacaggaatg ggtgagtgta gtcctcccga ttgtcgtcat cctggagaag atgaagtttt
     3901 tatgagaaat ttcgataatc acttatttta ttacgtgttc accaatctct ttgagggacg
     3961 gcttggagta gcttactata tagagaatcc agagggtggt actgcaataa gatttgggtt
     4021 ttctgcatac acaggatctc aaaattggcc tcttcggggg gagcatactg atgaagaaga
     4081 gccagatcat gatattttaa aacaaatcct ctccacgttc cggtttatgg aggaggaaaa
     4141 gtctattcaa gaaattgatt tcgcttctta cttctcatct ataccgcctt attccgattg
     4201 tgatgaggtt gcgccatctc tttctgccgt gtttgtgaac aatatcgaat acttcgacgt
     4261 tacagatgat ggtagggaag aggcaatcgt tatagcatct tcgtgttata cccatacggc
     4321 aggacctgat atcgtgggag tttatcaagc taatcttgat ggagaaatac agcctcttga
     4381 gtttaaggaa agtttgcaaa gccaccttat ccgctggaac cccaataaac aagttcttat
     4441 tggatttacc ggagcatacg aagcagggga tagaaattgt tgtccatcaa aggaaaaaat
     4501 tgttgagtac cagtgggata acaatattca acggtttagg atactgtcag aagagacgcg
     4561 cgaaagggta catagaaata aagcattttg ggacgacgat tgctatattg atacagtagc
     4621 ttgttagggt tgcctattgg tgagccctat ggggcaggtc ttgcagtttt cattctttcg
     4681 acagattcag ataacatttg gcaatacagg ttgaggccga tggtgtttac ggtgccggct
     4741 tgctcgcggc ctaagatatt gccggcgccg cgaatttcca aatctttcat ggcgattttg
     4801 tagccagagc ccagttcttc tgcttcttta agcgccttta aacgaagatt tgccttgggc
     4861 gttaatctgc cgccgtacag gaaatacgcg tacgactgga tgtttgacct gcccactctt
     4921 ccttttattt ggtaggcttg cgacaaacca agtttagtgg catcttctgc aataatagta
     4981 cttacgtttg ccatatccaa gccattttca ataatggtgg ttgcaattaa aacatcaaac
     5041 cttttggccc gaaaatcttc gataactttg ataagctggt cttctcgcat tctgccgtgg
     5101 gccacgccaa acgtggcgct gggtacgagt ctttctaaat gcttttttac ctgttctatg
     5161 gtgccaactc ggttgtgcaa ataatatgtc tgtccaccgc gggcaagctc tttttggata
     5221 gcttctgtaa taattttctc gtttcttttt gcaacagtgg tttgtatagg cagcctgcct
     5281 tcgggcggcg tttgaatggt gcttattttt ttgagcgaag agagcgccat gtacagggtg
     5341 cgggggattg gggtggcaga aagcgacaag acatccaaag agcttcgcat ttctttgaat
     5401 ttttcctttt gttttacgcc aaaccgttgc tcgtcgtcga gaacaagcaa acccaagttt
     5461 ttaaattcaa cgtcgtttga caaaagccta tgcgtgccaa ctacaatatc aatatttcca
     5521 ttttttaatt cttgcaaaac tttttcttgt tcttttctgc tttgcaaacg ggaaagcgca
     5581 gccaagcgaa taggcaagtc ttttaatctt gcttcaaagt ttttatagtg ctggtctacc
     5641 aatatggtag taggagccaa aagggctgct tgccatccgt tatgcgcagc gcgcaccatg
     5701 gctcgcaaag caacttctgt tttgccaaat cccacgtcgc cgcacacaat gcggtccatg
     5761 ggttcttctt gggcaaggtc ttcatatata tcctgtaagg cctggatttg gtctggcgtt
     5821 tcctcgtatg gaaaggtttc tttgagtttt tgttcaatat cttgtgcttc ttcgtacgcg
     5881 ggccgtgagc tgacctcgcg ctttgcgtaa atctctaaca gttctttggc cagccgctcg
     5941 atttcttctt ttgctttgcg tttggtcttt tgccaagtag gggagccaag gcgcgcaata
     6001 tggggatctg caaaccctac ataacgggaa agcttgcgtt ccaaaccaac gggcacgaat
     6061 agcttgtctt ctgccgcata ttccaaaacg taatactttt ctttgccaat ttcttcaaac
     6121 cccgtaaact ttgccacgcc gtggtctaag tgcaccaaat actcgttttc tttaaggttt
     6181 tttaggtctg aaaaaagcct ttgggatttt aaccgctttt gcaagatttc ttttgcttgt
     6241 tcctcatttg taacaaaaat aggcagttgc tctatagtat ctacgcgatt tccaacaaat
     6301 tccacccgaa tggcgttgct agagttaata ggaaaaaact ctaccatgcc gccttgatgc
     6361 gaaaactcgc ccatgtcgcg aacttccaga actttttcat agcccagctc gtctaacttt
     6421 cgtaaaagct ctgaaaccga atactgccgg tctttttcta aaaacaaaat attgtctgca
     6481 aaaaactgct gggtttttgc ggcctcgagt atttttttaa gattttcttt aaaccaaagt
     6541 cgcccctttt ccagaaagta gggggttatt gatgtaataa gaattgattc cttctgcgcc
     6601 tgtctgtcgg caggcaggtt tgacaaatcc atccgttcat agtatagcaa acctagcaaa
     6661 agaggcaata tgcctcaaat tctaaagtcg tagaaggatc gcatgaaagc agtatttata
     6721 ggcggggacg ggaggacctg tgaaattgcg actctggcca tgcgcctgcg ctggcccgat
     6781 gtctctcccg tcactatgcc caccgcagta gaaggactgg aaaccgtgga gcgcgagtcc
     6841 ccggacgtcg ttctccttca cccagacttt gacgacctta ccctggccaa ggccattcag
     6901 gaactccgcg gatttaccaa tgtacctctc ctggttctgg gccgaaaagg cgacgaaatg
     6961 gagttggtta cctctttgga actgggtgcc gacgactaca tcaggctgcc ctgtgacctt
     7021 accgagatca tgatccgcat ttgggccttg ttgcgccggg cgggcggtga aagcgaaggc
     7081 ccacttagga gcggccacct gtttgtcaac ccggccacct acaaggtttt tttgggggag
     7141 tgccgcgttt tcctcacccc tgccgagttc ctcttgctgc atctcctgat gaaaaaccgg
     7201 ggcatcgtga tgcccctcca gaccttggag cgtgccctcc gccacgaata tgtggatgtg
     7261 aacagcgggt tggtgaaaaa gtacgtccag cgcctccgcc gaaaactggg cgacaacgcc
     7321 tgggaacccc tctggataga caacgtctac ggcgtgggct accgatttgt tggcccacca
     7381 cccgaaaaac ctacctaaaa accagtcggc ggaacc
//
LOCUS       DASRUP010000023         5987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_684175, whole genome shotgun sequence.
ACCESSION   DASRUP010000023 DASRUP010000000
VERSION     DASRUP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684175"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFE94_01030"
     CDS             complement(<1..730)
                     /locus_tag="VFE94_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49717.1"
                     /translation="MLLVRRLFSFVTIFAILGAWMFSGWPQIWNDPAFPPQVKNALAA
                     TKTIEQQINIIDQEFSCGLSSCTSYSPDDNSLGIVHWDAAKYNGETVYFEAVIRNGLS
                     AESVEAALFSSSGSQVTGSEVTITSTSYTRVRSSALTLTDDTDYSVRGKRALGTVFIK
                     SARLIIVQSADPSITDTQTQVEVGNNESSTATSYTDLTDKKIYRYTSGSWSPTPTVYF
                     EASLKAVSTPTNEAFTSAGTTTGWE"
     gene            849..1559
                     /locus_tag="VFE94_01035"
     CDS             849..1559
                     /locus_tag="VFE94_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX49718.1"
                     /translation="MPRKPRQIEIGGIYHVVNRGVEKRIIFRKNQDYSRFVLGLEFFN
                     NEQTVDLWALLARGGTVPPRLQREAKKKPLVELMAFALMPNHYHLIVREIVQGGISLF
                     MRKMGGYSTYFNKQYERVGPLFQSRYKAVPIKTDEQLYTVFVYVHTNPAELKEPLWKD
                     YKVRSLEQTLAWLNEYRWSSYRDYIGKQVFPNVTQREFFINFLGGNKKCREAVEEWVK
                     IKARNAKLGGKIGPEIIE"
     gene            1631..2677
                     /locus_tag="VFE94_01040"
     CDS             1631..2677
                     /locus_tag="VFE94_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pesticidal protein Cry7Aa"
                     /protein_id="HZX49719.1"
                     /translation="MLKVEKHGVLLEPTEHIFERQAVLNPGVWQEGNNIHLFYRAIDE
                     DHCSSVGYARLKGPTQVVERWQKPILAGEYPYESKGMEDPRMVKIGDILHMFYVAHDG
                     KNALTAYATTEDLRHFVKKGIVSPTMSYHEFDLLNDELALKDAYSFFASYYEEGAGSD
                     VLIWHKDVFPFPRKIKGRYALLHRILPDMQIAFFENFNQLQDNAYWWEQLKRLPEHVV
                     LENKHWFETRSIGGGCPPVETKEGWLIIFHTTEERNKGRVYHACAALLDKDNPVKVLA
                     RLHEPLFSPTEEWEKQGFVSNVVFPTGTALFGDTLYIYYGAADKRIAVASVSVQELLK
                     EMKDPTKDHNHAAL"
     gene            2664..4856
                     /locus_tag="VFE94_01045"
     CDS             2664..4856
                     /locus_tag="VFE94_01045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX49720.1"
                     /translation="MQPYKTDNIAYLSTYPPRECGIASFTKDLVEAVQRKYNPALKPK
                     VLAINNDITDIYAYERQVIKQIAAENIEDYIQLAKELNDAPDLKLVHIQHEFGIFGGN
                     WGDYLIPFFQTVEKPVVVTLHTVLPRPNRTVLRLTQLICEHSKAVIVMNELSKEILLA
                     DYGAQESKVHVIPHGIPYVPTPNGQSEKAKLGLQNYLVLSTFGLLSPGKGIEYALRAL
                     PPLTKKYPNLLYLVMGVTHPVVRKHAGEAYRNFLLKEVNRLGLQNHVRFYNKYLAIDE
                     ITSFLRATDVYISPSLDRNQSVSGTLAYALGCGTPVVCTDSLYAKSIVTPNTGILVRP
                     KDAKVITKALRELCADPKLVKEMGKTAFAETRHMTWPNVAFSHFQLYQKLVPLQDEQK
                     LPPLNLAHLAKLTDGFGIIQFAKHTEPDLRYGYAVDDNARAMIVAAMAAKREYPGALD
                     LLKTYLAFVQFVQKPNGSFANLVNAKHKIPHQPESHDAQGRTLWGLGFVCGQEHLPAE
                     LRKTAGKLFQKALGTIVRIESPRAAAFAIIGLYWYLQTKQSVKARNLLEKLAERQLKR
                     FGETASKEWPWFEDSLTYSNSKLSESLLYAYAACKKKAYLSCAEKSLEFLIQTTFAKG
                     YFSSIGQNGWYARSGKRAYFDQQPEDACSMAQTLLVAYQITGKERYLKLAHDAFQWFL
                     GKNHLNQMVYDETTGGCYDGLGQQTINLNQGAESTISYLLARLAIEKP"
     gene            4853..>5987
                     /locus_tag="VFE94_01050"
     CDS             4853..>5987
                     /locus_tag="VFE94_01050"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HZX49721.1"
                     /translation="MTEQAKEKAIEVLEHCAKPTGFYASGLPGGYEATWARDSMVTGL
                     GASFVEKKFQKPFLKSIELLSKNQSALGQIPNAVGSYNTDRRSDVTFNSIDSSLWYLI
                     GHKAYAKAYSDTSLLKKYKKNIEKALLWLRYQDPNEDKLLAQQPTMDWQDAFPHKYGH
                     VINTQALYYGALRMYGKTKEAEHLKKVVNGEIEKYLTMYDENLGYYLPWNWKNHDGDR
                     EQEEWFDTLGNLLAIVTGLATPNIAESILNYIHIKQIDKPFPCRAIDPPMQPGDKEWH
                     SYFFKCDAKEPYAYLNGGVWPFIGGFYVAALVKTQQYAKAQEALEKLALADKQGKQGE
                     WEFNEWLHGQTGEPGPNSAPYQAWTASMYLFAAECVSQKRVPYF"
BASE COUNT         1700 a         1512 c         1417 g         1358 t
ORIGIN      
        1 cctcccagcc agtggtggtg cctgcacttg taaaagcctc attggtgggt gtcgaaactg
       61 cttttagcga tgcttcaaaa tacacggtgg gtgtggggct ccagctgccg cttgtgtagc
      121 gatagatttt tttgtcggtt aagtcggtgt acgatgtcgc ggtgcttgat tcgttgttgc
      181 ccacctccac ttgcgtttgc gtgtcggtaa tgctggggtc ggctgactgc acgataatca
      241 accgcgcgga tttgataaaa accgtcccga gagcgcgctt tcctctaacc gaatagtctg
      301 tatcgtcagt caatgttaag gcagaactcc gcactcgcgt ataactcgta gaggttattg
      361 tcacctcgct tccagtaact tgtgagccac tcgaagaaaa aagggctgct tcaaccgatt
      421 cggccgataa accgttgcga atgacagctt caaaatatac cgtttcgcca ttgtattttg
      481 ccgcgtccca gtgaacaatg cccagagagt tgtcgtcagg actatagcta gtacaagaac
      541 tcaagccaca actaaattcc tggtcgataa tgttgatctg ctgttcaata gttttggtgg
      601 cggccagcgc gttttttacc tgcggcggga atgccggatc gttccaaatc tgcggccacc
      661 cggaaaacat ccacgcgccg agaatcgcaa aaatggtaac aaagctaaaa agtcgccgca
      721 ccaaaagcat attgcattat tgtaccaaaa tttctgtttc ttgcgcaccc ccttatccac
      781 acttatccac aacaaccacg aggagggact gtccctcctc ggcttctgta tggtatagtt
      841 accataatat gccaagaaag cccagacaaa ttgagattgg ggggatctat catgtcgtta
      901 atcggggcgt agagaagcgc atcatatttc gtaaaaacca agattatagc cgttttgttt
      961 tagggttgga attttttaac aacgagcaaa cagttgatct ctgggcactc ctcgcccgag
     1021 gagggactgt ccctcctcgg ttgcaacgag aagccaaaaa gaaacctctt gtagaactta
     1081 tggctttcgc gttaatgcct aatcactatc atcttattgt gcgcgagatc gtgcaaggtg
     1141 gaatctcgct ctttatgcgc aaaatgggcg gatactctac ttactttaac aaacagtacg
     1201 aaagggtagg ccctctcttt caatcccgtt ataaggcagt acccattaaa acagatgagc
     1261 aactttacac ggtgttcgta tatgttcata cgaatccggc tgaactgaaa gaaccgctct
     1321 ggaaagatta taaggtaaga agcctcgaac aaacgctcgc ctggttgaat gagtatcggt
     1381 ggtcaagtta tcgcgattac attggcaaac aggtattccc aaatgtcacg caacgggaat
     1441 ttttcatcaa ttttttgggg ggaaacaaaa agtgccgaga ggcggtagaa gagtgggtta
     1501 agataaaagc gcgaaatgca aaactgggag ggaagatcgg ccccgaaatt atcgaatagc
     1561 ccgaggaggg actgtccctc ctcgggctgg cactcccttt caagaaaaga atctggtaca
     1621 atacgacaac atgctgaaag tggaaaaaca cggggtgctg ctcgaaccca cggaacatat
     1681 attcgaacgc caggcggtct tgaacccggg cgtatggcag gagggaaaca atatccacct
     1741 gttttaccgc gcgatcgacg aagatcattg ctccagtgtt gggtatgcaa ggctaaaagg
     1801 gccaacccaa gtggtagaac gatggcaaaa accaatcctt gcgggagaat acccctatga
     1861 gagcaaaggc atggaagatc cgcgcatggt gaaaatcggc gacatcctgc atatgtttta
     1921 tgtggcgcac gacggtaaaa acgcgcttac ggcctacgct acaaccgaag atttgcggca
     1981 ttttgtaaaa aagggcatcg tgagccccac catgagctat catgagtttg acttgctcaa
     2041 cgacgaactc gccctcaaag acgcctactc gttttttgct tcgtactacg aagaaggagc
     2101 agggtctgat gtgttaatat ggcataaaga tgtattccct tttccgcgga aaataaaagg
     2161 gcgttacgct ttgctgcacc gcattttgcc agacatgcaa attgcctttt ttgaaaactt
     2221 taaccagctg caagacaacg catactggtg ggaacagtta aaacgccttc ccgagcatgt
     2281 ggtgctcgaa aacaagcact ggtttgaaac acgaagcatt ggcgggggat gtccgcccgt
     2341 ggaaacaaaa gaggggtggc ttattatctt tcataccacc gaagaaagaa acaaaggccg
     2401 cgtataccat gcgtgcgccg cgctgctcga caaagacaac cccgtgaaag tattggctcg
     2461 cttgcacgaa cccttattct cgcccacgga agaatgggaa aagcagggct ttgtgtcgaa
     2521 tgtggtattc cccacgggaa ctgctttgtt tggagacacc ctatatatat actacggagc
     2581 ggcagacaag cgcattgctg tagcttccgt aagcgtgcag gaactgctca aagaaatgaa
     2641 agaccccaca aaagatcaca atcatgcagc cttataaaac agacaacatt gcataccttt
     2701 ctacctaccc tccaagagag tgcggtattg cgagttttac aaaagatctg gtggaagcgg
     2761 tgcagcgaaa atacaatccg gcgttaaagc ccaaagtatt ggcaataaac aacgatatta
     2821 ccgacatcta tgcctacgaa aggcaggtca tcaagcaaat cgccgcagaa aatattgaag
     2881 attacattca gcttgccaaa gaactcaacg atgctccgga tttaaagctc gtgcacattc
     2941 agcacgaatt tgggatattt ggcggcaact ggggggatta cctgattccc tttttccaaa
     3001 cagtggaaaa acccgtggta gttactcttc acactgttct tccccgaccc aatcgaactg
     3061 ttctgcggct cacacagctt atttgcgaac atagcaaagc cgttatcgta atgaacgaat
     3121 tgtcaaagga aatactgctg gcagattacg gcgcgcaaga atccaaagtg cacgttattc
     3181 ctcatggcat tccgtatgtg cctactccca acgggcaatc ggaaaaagcg aaactggggc
     3241 ttcaaaatta tctggttctt tctacctttg ggcttttaag cccgggtaaa ggcattgagt
     3301 acgctttgcg ggccctccct ccccttacaa aaaaatatcc caatcttttg tacctggtaa
     3361 tgggagttac gcacccggtg gtacgcaaac atgcgggaga agcataccgc aattttcttt
     3421 taaaagaagt aaatcgtttg ggccttcaaa accacgttcg cttttacaac aagtaccttg
     3481 cgatagatga aatcaccagt tttttgcgcg caaccgatgt gtatatttca cccagtctcg
     3541 accgcaacca atcggtttcg ggcacgcttg cgtatgcctt gggatgcggc actcctgtag
     3601 tttgcacaga ctctttatac gcaaaaagca ttgttacgcc caataccggc attctggtgc
     3661 ggcccaaaga cgcaaaggtc ataaccaagg cactgcgcga actttgtgca gatcccaaac
     3721 tggttaaaga aatgggcaaa accgcgttcg ccgaaaccag gcacatgaca tggcccaacg
     3781 tcgccttcag ccattttcag ctgtaccaaa agctggtacc cttacaagac gaacaaaaac
     3841 tcccgcccct taacctcgcc cacctggcca aacttaccga cggctttgga attattcagt
     3901 ttgcaaaaca caccgagccc gaccttcgct atggatatgc agtggacgat aacgcaaggg
     3961 caatgattgt ggctgcaatg gctgcaaagc gagaataccc gggcgcgctc gaccttctca
     4021 aaacatacct tgcctttgtg cagtttgtgc aaaagccaaa tggttcattt gcaaaccttg
     4081 taaatgcaaa acataaaatc ccccaccagc cggaaagcca cgacgcgcag ggcagaactc
     4141 tctgggggct ggggtttgtt tgcggccagg aacaccttcc cgcagagtta cgcaaaacag
     4201 caggcaaact ttttcaaaaa gcgcttggca ccatagtacg catcgagtcg ccccgagccg
     4261 ctgcatttgc cattattggc ctgtactggt atttgcagac aaagcaaagc gtgaaggcgc
     4321 gaaatttgct cgaaaagctc gccgaaagac agctcaagcg ctttggcgaa accgccagca
     4381 aagagtggcc gtggttcgaa gacagcctta cctattccaa cagcaagctt tcagaatcgc
     4441 ttttgtacgc ctatgccgca tgtaaaaaga aagcgtacct ttcgtgcgcg gaaaaaagcc
     4501 tggagttttt aatccagacc acattcgcca aaggctattt ctcttccata gggcaaaacg
     4561 gctggtatgc gcgctcgggg aaacgcgcgt atttcgacca gcagccggaa gacgcctgtt
     4621 ccatggcgca aacattgctg gtagcctacc agattaccgg aaaagaacgc taccttaaac
     4681 tggcgcacga cgcgttccag tggttcctgg gcaaaaacca cctcaatcaa atggtatacg
     4741 acgaaacaac cgggggatgc tatgatggat taggccagca aaccataaac ttaaaccagg
     4801 gggcagagtc tacgatttcg taccttcttg cccgtttggc aatcgaaaaa ccatgacaga
     4861 acaagcaaaa gaaaaagcaa ttgaggtact cgaacattgc gccaaaccca cgggctttta
     4921 cgcctcgggc cttcctggcg gatacgaagc cacatgggcc agagacagca tggtaactgg
     4981 gctgggcgcc tcgtttgtcg agaaaaaatt ccaaaagcct ttcctcaaaa gtatagaact
     5041 cttgagtaaa aaccagtctg cgctggggca aatccccaat gccgtaggaa gttacaatac
     5101 cgaccggaga agcgacgtta ccttcaactc cattgattct tcgctttggt atcttattgg
     5161 ccacaaggcg tatgcgaaag cctacagcga tacctccctg ctcaaaaagt acaaaaagaa
     5221 tatcgagaaa gctcttcttt ggctgcggta ccaggatcca aacgaagaca agcttttggc
     5281 ccaacagcca accatggact ggcaagacgc ctttccccat aaatatgggc acgtgataaa
     5341 cacgcaggct ttgtattacg gcgcgcttcg catgtacggc aaaaccaaag aagcagaaca
     5401 cctcaaaaaa gtggtaaacg gagaaatcga aaagtatctt accatgtacg acgaaaacct
     5461 cgggtattac cttccctgga actggaaaaa ccacgatggc gaccgcgagc aggaagagtg
     5521 gttcgatacc ttaggaaacc ttttggccat tgtaaccggc cttgccacgc caaacattgc
     5581 agagtctatc ttgaattaca tccacataaa acaaatcgac aagccctttc cctgccgggc
     5641 catcgaccct cctatgcagc caggcgacaa agaatggcac tcttatttct ttaagtgcga
     5701 cgccaaagaa ccttatgcct atctcaacgg aggcgtgtgg ccgtttatcg gcggctttta
     5761 cgtagcagct ctcgtcaaga cgcagcaata cgcaaaagca caagaagcct tagagaagct
     5821 cgcgcttgcc gacaaacaag gcaaacaagg agaatgggaa tttaacgaat ggctgcatgg
     5881 ccaaaccgga gaacccggcc ccaacagcgc gccctaccag gcctggactg ccagcatgta
     5941 cctcttcgcc gccgagtgcg tttctcaaaa acgagtccca tatttct
//
LOCUS       DASRUP010000024         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1950289, whole genome shotgun sequence.
ACCESSION   DASRUP010000024 DASRUP010000000
VERSION     DASRUP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950289"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="pyrB"
                     /locus_tag="VFE94_01055"
     CDS             complement(<1..899)
                     /gene="pyrB"
                     /locus_tag="VFE94_01055"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZX49722.1"
                     /translation="MNQMHHVVAAKQLAEKALLKQLFELADSFAAGKNNNSLKGKVLA
                     TLFYEPSTRTRFSFEAAMLRLGGQVISSENAFEHSSAKKGETLQDSIRIVASYADCII
                     LRHPETGAADKAAAVSSVPIINAGDGSGEHPSQALLDVYTIQKELGRLDNLNIVLVGD
                     FLYGRTVHSLVQLLDIYDNIQLSLVAPLQLALPAKYKRGLKNRIVETTDFQSVLPEAD
                     VVYMTRVQKERFQTKGEYENLKAAYVFGEKELSLLKKEAIIMHPLPRVDEISQAVDAD
                     PRAAYFRQAQNGLYVRMALLHYL"
     gene            complement(841..2118)
                     /gene="pyrC"
                     /locus_tag="VFE94_01060"
     CDS             complement(841..2118)
                     /gene="pyrC"
                     /locus_tag="VFE94_01060"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZX49723.1"
                     /translation="MIKSIEGNFVNFDRSFYGRVELDSKTGLIEKIAEPLGEADLVVE
                     NGLIFPGFVDLHVHVREDPSKKQTYKEDFQTASKAALHGGVVCALDMPNNPEPPVDEK
                     TYLAKKKLAEGKPVDFVLYAGVKPGSTPLSLHVPYKVFMAHSVGDLFFKNFDQLEKTL
                     SLYRGQAVSFHCEDPELISKTKRPPEAEISAIGFALFLIQRYELQGNICHCSTKAGIE
                     KIKEAKRKGVRVTCEVTPHHLYFDETLLDNKNRKWLRVNPPIRTATDRKALLDALREG
                     TIDYLASDHAPHTKHEKDDNQLAGETGSAGMPHLDTYGNVVTWLMAEQDFSAKDIARV
                     CAYNPGQFVNQFTQTKYGKITEGYAGSFTVIDPKRPMVVLEKDLQTKCGWSPFSGVEF
                     PGQVLYTVQKGEIAYESNASRSSSKAAGREGAA"
     gene            complement(2115..3080)
                     /locus_tag="VFE94_01065"
     CDS             complement(2115..3080)
                     /locus_tag="VFE94_01065"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZX49724.1"
                     /translation="MTKDSSGEKQHEQWLQGLQETKEYAFDRPALHTEVFGLQFANPI
                     GLAAGFDKHATALPQLASLGFGFLEVGTVTPKAQPGNPKPRMFRIPEEQAMLNRMGFN
                     SNGAEWLAEKLNSQKLAVPLGINIGKNKDTSNERATEDYVECFRMFAQFAEYIAINVS
                     SPNTPGLRDLQEKAFLQNLLARLPKQFPVFVKIAPELSNAQLQDIVDVVGNREHTGII
                     ATNTLKTEEGGISGKPLRNRSTHVIRELYRISKGEIPIIGVGGIFSADDAYEKIRAGS
                     CLVQLYTGLEYQGPGLIKKLKQGLVELLQRDGFASVGQAVGKDNV"
     gene            complement(3085..>3637)
                     /locus_tag="VFE94_01070"
     CDS             complement(3085..>3637)
                     /locus_tag="VFE94_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX49725.1"
                     /translation="VLFSIVYFREHLSIYQIAGIGLAVIVFVLLTRHNHEERARHADF
                     RAGVILAVIALVTGAGATIISKFAAESFEEFFSFIALSNAINIFLAFGIKNKLQRERE
                     NPKHTNAIIIGVLSGLFNFVGYYAIMHALAAGPLSIIAAVHSMNFVIAILLAVLIYKE
                     ELTKTRLAGIGLTVLALILLRLS"
BASE COUNT          858 a          896 c          868 g         1015 t
ORIGIN      
        1 aaaagatagt gtagtagagc catgcgcaca tacaggccgt tttgggcttg gcggaaatag
       61 gcggcgcggg ggtctgcatc cactgcctga gagatctcgt ctacccgggg caaagggtgc
      121 ataatgattg cttctttttt caacaaacta agttcctttt cgccaaatac atatgcagcc
      181 ttaagatttt catactcccc tttcgtttgg aaacgctctt tctgcacgcg cgtcatgtat
      241 accacatctg cttctggtaa aacactttga aaatcagtgg tctctacgat gcggtttttt
      301 aaaccccttt tatattttgc gggtagggca agctgcagcg gcgccacaag cgaaagctgt
      361 atgttgtcgt atatatccag caactgcacg agtgagtgta ctgttctgcc atacagaaaa
      421 tcccccacca gcacaatgtt gagattgtcg agccgcccga gttctttttg tatggtatat
      481 acgtccagta aagcctggct ggggtgttcg cccgagccgt caccggcatt tataataggc
      541 actgaagaaa cggcagcagc cttgtcggca gctcccgttt cggggtggcg caatataatg
      601 cagtcggcat acgaggctac aatgcgtatg gagtcttgga gcgtctctcc cttttttgcc
      661 gaagaatgct cgaatgcgtt ttccgaactt atcacctggc cgcccaaacg cagcatagct
      721 gcctcaaacg aaaaccgagt tctcgtactc ggttcgtaaa acagcgtggc caaaactttt
      781 ccttttagag aattgttgtt cttcccagcc gcaaagctat ctgccagttc aaataattgt
      841 ttaagcagcg ccttctctgc cagctgcttt gctgctacta cgtgatgcat ttgattcata
      901 agcaatttcg cccttttgaa cagtatagag tacctggccg gggaattcca cccctgaaaa
      961 aggcgaccac ccgcattttg tctgcaaatc tttttccaga accaccattg gcctttttgg
     1021 atcaattacc gtaaaagacc ccgcgtagcc ttcggtaatc ttgccatact ttgtttgtgt
     1081 aaactggttt acaaattggc ctgggttata ggcgcacacg cgggcgatat ctttcgcgct
     1141 aaagtcttgt tctgccataa gccacgtaac aacattgcca tacgtgtcga gatgcggcat
     1201 tccggcgctg cctgtttccc cggcaagctg attgtcgtcc ttttcgtgtt ttgtatgcgg
     1261 ggcatggtct gaagccagat agtcaatggt gccttcgcga agggcgtcga gaagggcttt
     1321 ccggtctgtc gcggtgcgga taggcggatt tacccgcagc cactttcggt ttttgttgtc
     1381 gagcaaagtt tcatcaaaat acaggtggtg gggtgtaact tcgcaggtta ccctaactcc
     1441 ttttctcttt gcttccttga ttttctctat gccggctttt gtggagcagt ggcatatgtt
     1501 tccttgcaat tcgtaccgtt gaataagaaa aagcgcaaaa cctatggcgc ttatttcggc
     1561 ttcgggcggc cgtttggttt ttgaaatgag ctctggatcc tcgcagtgaa aacttaccgc
     1621 ctggccgcgg taaagcgaga gggttttttc gagctggtca aaatttttga aaaacaaatc
     1681 ccctaccgaa tgggccataa aaaccttgta gggaacatgc aaagaaagcg gcgtgctgcc
     1741 aggcttcacg ccggcgtaca aaacaaaatc aacgggtttg ccctctgcga gtttcttctt
     1801 tgcaagatag gttttttcat ccacaggcgg ttcagggttg ttgggcatgt cgagggcgca
     1861 taccacgcct ccgtgcagcg cagccttgga agctgtttgg aagtcttctt tgtaggtttg
     1921 ttttttgctt gggtcttctc tgacgtgaac gtggagatcg acaaagcctg gaaaaataag
     1981 gccgttttcc actaccaaat ccgcctcgcc caggggctct gcaatttttt cgataaggcc
     2041 ggttttgctg tcgagctcca cgcggccgta aaaactgcgg tcgaaattca caaaatttcc
     2101 ctcaatgctt ttaatcatac attgtctttt ccaacggcct ggccaaccga cgcaaagcca
     2161 tcgcgctgca gcaattccac caggccttgc tttagctttt ttataagccc tgggccttgg
     2221 tattccaaac ccgtgtacaa ttgtacgagg caagagccgg cccggatttt ttcatatgca
     2281 tcgtctgcgg aaaaaatgcc gcctactcca attataggta tctcgccctt cgatattcgg
     2341 tacagctcgc ggattacgtg cgtggagcgg ttgcgcaatg gctttccgct aatgcccccc
     2401 tcctcggttt ttagagtgtt ggttgcgata attcctgtgt gctctctgtt tcccacgacg
     2461 tctacaatat cttgaagttg ggcgttcgaa agctccggcg cgatttttac aaagaccgga
     2521 aattgtttgg gcagtctggc caataagttt tgcaaaaaag ccttctcctg taagtcgcga
     2581 agtcctggtg tgtttggcga gcttacattt atggcaatat actctgcaaa ttgagcgaac
     2641 atgcgaaaac attcgacata gtcctcggtt gctctttcgt tgctcgtgtc tttgtttttg
     2701 ccaatgttta tacccaaggg taccgcaagt ttttgcgagt tcagtttttc tgccagccat
     2761 tctgccccgt tgctgttaaa ccccatgcgg ttgagcatcg cttgttcttc tggaatgcga
     2821 aacatgcgag gctttgggtt gccgggttga gcttttggcg ttacagtccc cacctccaaa
     2881 aagccaaatc ctaatgatgc taactgcggc aaggctgtgg cgtgcttgtc gaatcccgcg
     2941 gccaaaccta tgggattggc aaactgcaag ccaaaaactt ctgtgtgtaa agcagggcgg
     3001 tcgaacgcat attctttcgt ttcttggagc ccttgaagcc attgttcgtg ttgtttttcg
     3061 ccagaggaat ccttagtcat acatttatga aagtcgcaac aaaataagcg ccagtaccgt
     3121 aagcccaatg cccgcaaggc gcgttttggt aagctcttct ttataaatga gcactgccag
     3181 caaaatcgca ataacaaagt tcatgctgtg tacggcagca ataatgctca agggccccgc
     3241 ggcaagcgcg tgcataatag cgtagtagcc tacaaaatta aacaaccccg aaagaacgcc
     3301 gataataatt gcgtttgtat gctttggatt ttcgcgttct ctttgcagtt tgttctttat
     3361 gccaaacgcc aaaaaaatgt tgattgcgtt ggataaagca ataaacgaaa agaattcttc
     3421 aaacgactct gcagcaaact tgctaataat agttgccccg gctcccgtta ccaatgcaat
     3481 aacagctaaa atgacaccgg cccgaaaatc agcgtgcctg gcgcgctctt cgtgattatg
     3541 ccgcgtaagc agtacaaaca cgataaccgc aaggccgatg ccggctattt ggtatatcga
     3601 aagatgttcg cggaaatata caatggaaaa cagcaca
//
LOCUS       DASRUP010000025         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1627210, whole genome shotgun sequence.
ACCESSION   DASRUP010000025 DASRUP010000000
VERSION     DASRUP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627210"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /gene="msrA"
                     /locus_tag="VFE94_01075"
     CDS             complement(<1..432)
                     /gene="msrA"
                     /locus_tag="VFE94_01075"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZX49726.1"
                     /translation="MASVQFQTAVFGGGCFWCGEAVLQRLKGVQSVTSGYAGGSMENP
                     SYQQVSSGHSGHVEVIKIEYDPAVLSYDTLLSVFFATHDPTTTNRQGSDIGPQYRSAI
                     FYTSEEQKTETEKFMQKLADDEVFKDPIVTELKPLDNFYQAE"
     gene            complement(438..953)
                     /locus_tag="VFE94_01080"
     CDS             complement(438..953)
                     /locus_tag="VFE94_01080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZX49727.1"
                     /translation="MTKQIRTKRLLLRQYKKGDEESFLANINNPKVSRWTSGIPYPYS
                     KEDLETWQNILAKRQGKTEISFVITRENEVIGGVSLRGIRKGHKARIGYWLGEAYWGQ
                     GFMVEAIYAITKLGFEQFGLHRIEAKVFLPNKGSKRVLEKAGFRLEGLLKKEYLKNGK
                     FIDAYLFAKVK"
     gene            1010..1717
                     /locus_tag="VFE94_01085"
     CDS             1010..1717
                     /locus_tag="VFE94_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZX49728.1"
                     /translation="MIKLVSINIELDKHLDLVENFLKEEKPDVACLQEIYETDLQNLA
                     TKLGMTPLFGQMALMGRFPFGIGILSRVPFESVSRAYYFGDKETAPTHTYNGEPQSFY
                     RLLLSASFQKEGTSAVIGTTHFTWTPNGETDETQKAHLASLLEVLQGIPEIVFCGDLN
                     APRGREIFDTLATSYKDNIPQRYTTSIDKNIHHAGNLQLMVDGLFSTPAYTLKNVRLE
                     QGVSDHLAIVAEISHSS"
     gene            complement(1705..2505)
                     /locus_tag="VFE94_01090"
     CDS             complement(1705..2505)
                     /locus_tag="VFE94_01090"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HZX49729.1"
                     /translation="MKQIFIPIGIIALVAIVAGGALYFYQQQQAPTVEEPGNNNGVAV
                     SNFAECEAAGYPIMESFPRQCRTPQGQNFTEDIGNELEKTDLIRVDNPRPNQEIASPL
                     MIEGEARGTWYFEASFPIRLLDEEGNELAVAIAQAQDEWMTEEFVPFKATLEFDTPET
                     SVGTLVFQKDNPSGLPEHDDELLMPIRFGEARLSRAQDGCVITGCSGQVCADEEQITT
                     CEFRPEYACYNSAVCARQTSGQCGWTQTAELTQCIENAKGSAQQLQEE"
BASE COUNT          615 a          702 c          557 g          722 t
ORIGIN      
        1 ttctgcctgg taaaagttgt cgagaggttt gagctcggta actatgggat ctttgaaaac
       61 ttcgtcgtct gcaagctttt gcataaactt ttctgtttcc gttttttgct cctcactcgt
      121 ataaaaaatg gcggaacgat actgcggccc tatatcactc ccctgccggt tcgtagtggt
      181 cggatcgtgc gtcgcgaaaa ataccgacag caaagtgtcg tatgaaagaa ctgcgggatc
      241 atactctatc ttaattacct ccacatgtcc gctatgcccg ctcgacacct gctggtagct
      301 cggattttcc atacttccac ctgcgtatcc ggaagttaca ctttgcactc ccttcaaacg
      361 ctggagtacc gcctcgccac accaaaaaca cccgccccca aataccgctg tttgaaattg
      421 cacagaagcc atatctatta ttttaccttt gcaaataaat aagcgtcaat aaactttcca
      481 tttttcaagt actctttctt gaggagtcct tcgagcctga accctgcttt ttcaagtacg
      541 cgctttgatc ccttgtttgg caaaaaaact ttggcctcga ttctgtgcaa gccaaactgc
      601 tcaaacccaa gctttgttat tgcgtaaatt gcctcgacca taaagccttg accccaatag
      661 gcttcgccca gccagtaccc tatgcgggct ttgtgacctt tgcgaatccc ccgcagacta
      721 actccgccaa tgacttcgtt ttcgcgcgtg atcacaaacg agatttctgt tttcccctgt
      781 ctctttgcca aaatattctg ccaggtttcg agatcttctt tgctgtaggg atacggaatt
      841 ccactcgtcc agcgcgagac tttgggattg ttgatgttgg ccaaaaaaga ttcctcgtct
      901 ccttttttgt actgcctaag aaggaggcgt ttcgtgcgta tctgctttgt cataacctaa
      961 ccatagcaaa gagaaaaaat atatgctata gtagttgcga cgcattccta tgataaaact
     1021 cgtttctata aatatcgaac tcgacaaaca tctcgacctg gtagagaatt ttctgaaaga
     1081 ggagaagcca gatgtggcgt gcctgcagga aatatacgaa accgatctgc aaaacctggc
     1141 aacaaagctt gggatgactc ccctttttgg ccaaatggct ttgatgggcc gtttcccttt
     1201 tggcattggc atactttccc gggtaccttt tgaaagcgta tcacgggcct attactttgg
     1261 cgacaaggaa accgccccca cccatacata taacggggag ccgcagtctt tttatcgcct
     1321 tctgctctct gccagttttc aaaaggaagg cacaagcgct gttatcggca ccacgcactt
     1381 tacctggacg ccaaatggcg aaaccgacga aacgcaaaag gcgcaccttg cctctctttt
     1441 agaagttctc caaggtattc cggaaatcgt tttctgcgga gacctcaacg ctccccgagg
     1501 aagagaaatt ttcgatacgc tcgccacgag ctacaaagat aatatcccgc agcgctacac
     1561 cacaagtatc gacaaaaaca tccatcatgc aggaaacctg cagctcatgg tagacggcct
     1621 ttttagcacc ccggcctaca ccctcaaaaa tgttcgcttg gagcaaggcg taagcgacca
     1681 cttagcaatt gtcgccgaga tttctcattc ttcctgaagc tgctgcgcag atccttttgc
     1741 gttttcgatg cactgggtaa gttctgcagt ttgcgtccag ccgcattggc ccgatgtctg
     1801 gcgggcgcat acggccgagt tgtagcatgc gtattctggc cgaaattcgc aggtggtaat
     1861 ttgttcctca tctgcacaca cctggccgga acacccggta attacgcagc cgtcttgcgc
     1921 gcggcttaac ctcgcttctc caaagcgaat gggcataagc aattcgtcgt cgtgctctgg
     1981 caggcccgaa ggattatctt tttggaacac aagcgtgccc accgaagtct cgggggtatc
     2041 aaattcgaga gttgccttaa aaggcacaaa ttcttcggtc atccactcgt cttgcgcttg
     2101 cgcaatggct accgcaagct cattcccctc ttcgtcgagc agacgaatcg ggaaagaggc
     2161 ctcgaaatac catgttcctc tcgcttctcc ctcgatcatc aaagggcttg cgatttcctg
     2221 gttagggcgc ggattatcca cacggatcaa atccgtcttt tctaactcgt ttccaatatc
     2281 ttcggtaaag ttctgcccct ggggcgtacg gcattgcctg ggaaaactct ccataatggg
     2341 gtatcccgca gcctcgcatt ctgcgaaatt gctcactgct accccgttgt tgttccccgg
     2401 ctcttctaca gttggcgctt gctgttgctg gtaaaaatag agtgcgccgc cagcaacaat
     2461 tgccaccagc gcgataatgc ctatgggtat aaaaatctgt ttcattttct tcttctggtt
     2521 tccactaatt tatctttgta tcgacgtatt tcttttttta atttttcctt tacctggtct
     2581 atggccaaac gaatat
//
LOCUS       DASRUP010000026        14171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_729254, whole genome shotgun sequence.
ACCESSION   DASRUP010000026 DASRUP010000000
VERSION     DASRUP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729254"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFE94_01095"
     CDS             <1..222
                     /locus_tag="VFE94_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49730.1"
                     /translation="GGDGGEGWNGGMGGDGGMGGEGDNGGDAGNGGDGAEGGDGGDGG
                     GDGLITTGDATSTGRVVNIVNRNMTRVRR"
     gene            303..728
                     /locus_tag="VFE94_01100"
     CDS             303..728
                     /locus_tag="VFE94_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49731.1"
                     /translation="MKFIQNTILFFCLFGVLGTAHATTVQNEISVSASTGGNTAGQET
                     QGKSEVSVFIETTVDGEVVEHIEEHKEDTQGNPVEIRKETSYQDGKVQVQTQSAVEVL
                     PEDNANKNVEEVEPENESKPLFVALLDFIRDIFSNLFFA"
     gene            725..3943
                     /locus_tag="VFE94_01105"
     CDS             725..3943
                     /locus_tag="VFE94_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49732.1"
                     /translation="MKRFNFHVTFVVACLLFVALNLYTVDFPVFAQDAGSEVVQEDGK
                     DGKDGEKGQEGEDGQEGQDGEQATDGQEGTDGKEGEEGQAGEPQDDPPAEDGENGDPA
                     SDGESGDSGEPGDNGDNGGEEENGEDGGDATDGEDATDGEDGGDGEYGENGEDATGGE
                     EDSQGEAQVETGDATSAGQIQNTANSNSVNSKEPGSEELPFCSDKTRKDEGKIEDEGK
                     GSKEDSQDEEAPYCVEENSSGGSGGEMEVENTNAADIQNGAEAGAETGENEASNNAGD
                     ATIDTGDATAVLDLFNLINTNVINSQGLFVLLSNLQQQLLDIDLRDWMPYLQDSTDCD
                     PAQCVRLQELLVKNQNIANIVNELAVYAGTGGNEANDNTGDASIVTGDAYAAANIINI
                     ANTNIINSNYLLFIANNFGNFFGDIVFPNSSSFLQALMASPSQVYSEVEIDNTNNASV
                     SNNVGVDAQTGGNEANGNGTSLVQTGDANAVANVFNQINMNLFGGNSFVALVRVSGNW
                     SGDIFGLPAGISWVQTPGGIMLYSDDLSPLGGSPEGGELGTAAEDSSSETGDQPGEEG
                     KSGKGSKKDHSELSVENENNASITNNVQVYALTGENKASENGGNASIQTGDALAVANI
                     VNIANTNVIGKNWLFAIFNILGDFDGNISFGRPDLWIGARAEIPHDPLLPGDEFTYYL
                     TVANNGDAPANQVRITNVFDDGFLAFGSSLDNTGLDANKVFWDLGTLNPGEGREVSFT
                     ASIRTDIPYGVHEFMNTSAIGAKESDDNNQDNTDSVELAVFHEEPAPPSGGGGNPPNN
                     DSSDGGDGDNNGNDNGGNNSDGDGGNDTDSGNSDSNTDSSSQNNVSPTQRTNANPSDS
                     TKAPNLHVQKNFVGEGVVVPGDSVDYTIVVTNTNGGSAYDAILVDTLEDQDGNVVNEE
                     IWDLGEVFPDEEITLTYTVVFDDETEAGLYTNRAQAVAYEGSVDGKEVSSNIASVGIL
                     VEWESPAPQESILSLANSFQQGSGFALASPQPSGAGEPGQEAPAQANEDPFVFSNEET
                     RPFYASLAGLLNDLSGWLIALASAVALFLLVFLLKRRRSQQA"
     gene            3997..5685
                     /locus_tag="VFE94_01110"
     CDS             3997..5685
                     /locus_tag="VFE94_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZX49733.1"
                     /translation="MKTLSKQLSIFCVISLVVTSGVFFPVVNAQGELREAREVREVRA
                     AREEGVVVVKFHGSDREQRIRLSAGETVEAALQRYRQRQDVAYAEPNYIAEAFGVPND
                     PYYSYQWHLSQIGLEAAWDISTGSGAVVAVIDTGVAQLSDLAGTSFVQGYDFVNNDSN
                     PTDDNGHGTHVAGTIAQSTNNSLGVAGVAYGATLMPIKVLNKSGSGTYADIADAIRYA
                     TDNGARVINMSLGGPSPATYLQDALQYAYGKGVTIVAAAGNDGQNSISYPAAYNDYVI
                     AVGATRFDKARAPYSNYGSGLDVVAPGGDTSVDQNGDGYGDGVLQQTFSSTPNSPGYY
                     FYQGTSMATPHVAGVAALVISNGNAQTPADVREALQSSAEDLGSAGWDSSYGHGLINA
                     ASALAWSSSGSPTPPPSPSPPPPAEIEVFSESFEQGLGSWTQDSQNDWFRSSQRDMEG
                     RYSAEVDGSASNASIISSAIDLQGKTAAKIAFSWYIERGLDSGEYLAFDISTDGGTTW
                     VEKARLRGNQDPENSWRQVQTDLSGISNLKLRFRGTMSGSAEDANVDNVKVTAY"
     gene            5707..6513
                     /locus_tag="VFE94_01115"
     CDS             5707..6513
                     /locus_tag="VFE94_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZX49734.1"
                     /translation="MIEGIVLGTIQGITEWLPISSEAAIILVKTRFFPNGEGLADLIG
                     LAVFLHLGTFLAAAWYFRDDVIMLARSLFLPHKLFQREERFLILRFLIVSTFISGILG
                     YFLIEMVVPRAEEHIELGAKAVTLGIGILLVITGFFLLQAKGKKGQRGVIHLRDRDGV
                     LLGFVQGFTALPGFSRSGLTISTLLLRKFEEISALRLSFLMSLPAVLGGNILLNARGF
                     EITLTAIFGLIFSFLFGILTMHLLFKVAKKVNFGWFVLIFAALVIASVFV"
     gene            6556..6798
                     /locus_tag="VFE94_01120"
     CDS             6556..6798
                     /locus_tag="VFE94_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49735.1"
                     /translation="MKAFIVRPWVPIEETPAQTKGDRKHLKRKIGAQAPPPVADPVEA
                     EKTSSDSYAKIRADYYAGRLKHLNQETLQQVVDAFL"
     gene            6863..7555
                     /locus_tag="VFE94_01125"
     CDS             6863..7555
                     /locus_tag="VFE94_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZX49736.1"
                     /translation="MEKFLVISSHPDDLEFSCGGTVAKLRKDGKQVEYLIVADGSKGS
                     HKVGFDGKKLAQLRQKEQKTAAKTLGVEKVTFLGEVDGVVENTPQLRKKLVKEIRQAK
                     PDVVLSFDPSSLDFENVYLAHRDHRMAAEAAFDALYPAVGSGAFFPELLKEGIRPHQV
                     KEIWFFATSRPNKWVDITATIGLKLQALFCHESQLEDAKKFEEIIRKSAAKEGKQKKI
                     KYAECFRVIDWG"
     gene            7646..7813
                     /locus_tag="VFE94_01130"
     CDS             7646..7813
                     /locus_tag="VFE94_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HZX49737.1"
                     /translation="MATEGYCVKCKAKREMTNEQKTEMPKKGGGTRPAMKGTCPVCGT
                     GMFRILPMSEA"
     gene            7939..8346
                     /locus_tag="VFE94_01135"
     CDS             7939..8346
                     /locus_tag="VFE94_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49738.1"
                     /translation="MISRRKAGQITDYDISEIWKTVLFGIPGLGGLPDLNAVVTAIYP
                     TDTLDVAREKMACSFSTTALTLFQDLFKERVDLNEYSGTTRDFVKKLQGKTHSFEDVE
                     VALEKFATSQGVPFNNHRHVPTLYWKLPSAIPK"
     gene            8403..9248
                     /locus_tag="VFE94_01140"
     CDS             8403..9248
                     /locus_tag="VFE94_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX49739.1"
                     /translation="MKKVGYLGIIGLALANIVIWPYVVEGSNLRVTFFDVGQGDAIFI
                     QTPQGHQMLIDGGPDQKVVEKVAKVLPFWDKSLDLVVLTHADADHITGLVSVLEQYDV
                     GNVLWTGVEKETNIFAAWEQELAQERANIWTADILQTILWSKDRSARFDVVYAAKEAK
                     EINDTSVITKLVFGETSFLFPGDISKNVEQRLLQEKLAIDADILKIPHHGSKSSSSEA
                     FLAAVSPYMAVIQVGGQNSYGHPAFEVLERLFAMGTTTLRTDQNGDIVIEYDSSKGRT
                     NISNY"
     gene            9208..9813
                     /locus_tag="VFE94_01145"
     CDS             9208..9813
                     /locus_tag="VFE94_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011849208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZX49740.1"
                     /translation="MTHPKEERTLVIIKPDGIQRSLVGEIIKRYERVGLKLVGMKMLV
                     PQENKVEQHYLLDPEWKRKVGEKAIESYQKKGVEPPSKNPIEVGDRVVAGLKKYLTAG
                     PVVAMVWEGAHAVEIVRKLTGGTEPRSSDVGTIRGDFVLDSYQMGDADNRAVRNLIHA
                     SGSVQEAEAEIAHWFAKEELVNYKIVQEKILYDVNLDGILE"
     gene            10015..10569
                     /locus_tag="VFE94_01150"
     CDS             10015..10569
                     /locus_tag="VFE94_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HZX49741.1"
                     /translation="MNPIFKHIQNRSLAHVLRFNLKPQHFEESVADHSYFVAYIVSVL
                     CYLLERQGEQVDAKKALEMALVHDMEEIFSGDIVTPLKHYSSEVEEAITKVNKEMIPE
                     VFSGLPEELSAHFISLWTEEGQGQTMEAQVVKVADKLSLLSKCAEEVHVGNEFFQEIY
                     EKGMRFLEEYDKPWWQKIKAQILP"
     gene            complement(10547..10855)
                     /locus_tag="VFE94_01155"
     CDS             complement(10547..10855)
                     /locus_tag="VFE94_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49742.1"
                     /translation="MLGIDMRFQIPLSGKNIHTLMRQAGYAPGFSQSSEEPAFQRPGG
                     NTYPKFHVYAKTENGSARLNLHLDQKRPSYGGTHAHAAEHDGPVVEAEAARIKEVSAP
                     "
     gene            10924..11397
                     /locus_tag="VFE94_01160"
     CDS             10924..11397
                     /locus_tag="VFE94_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZX49743.1"
                     /translation="MHQKIRNFRYPIGGQQGKVVPMVLIFVTLLVAGAGAVMLLKTRN
                     TVIVINEAATAPQERVVTESPQPQAEAPEPVPQVRISQTSVGYLNVRKSGSAQAEKIG
                     EVKPGEVYEFTEVVNNWYHIVHPTLGEAAWVSGQYVTEVEAEAGNSNIFSPPSEI"
     gene            12148..13410
                     /locus_tag="VFE94_01165"
     CDS             12148..13410
                     /locus_tag="VFE94_01165"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HZX49744.1"
                     /translation="MKKILMSLGVIVAVGAIVAGATGAFFSDSETSVGNTFTAGAIDL
                     GIDNESYYNGVLNDGTTWDLSFDLDECEIRNPEYNSDTDQDGDGIDADGDPEFFPCLF
                     FNFDDLKPGDYGEDTISVHITNNEAWLCADVTLTKNDDMSCTEPENDVDAENGACVDP
                     GPGLGDLAQEIDFLWWADDGDNVLEDDETPLPAGKLGSLGVGNTATVPLADSNGNIWT
                     GNPDDPILTDEEDEILYIGKAWCFGDITPAPVPQDGFGDQMTPAGDNEGAGNVGNGTL
                     GQPSDGGYACDGEGVGNEPQTDQAMLNVSFRAVQARHNDDFQCVPQQQEEPAQLTVIK
                     EVIPSTAFDQDLGVGDFDLFVDATAVVSGVANVFGTGAHVVSETAVAGYSSAISGDCD
                     AAGNVTLNPGDNKTCTITNTEVADASTL"
     gene            13503..>14171
                     /locus_tag="VFE94_01170"
     CDS             13503..>14171
                     /locus_tag="VFE94_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HZX49745.1"
                     /translation="MKKILFSLAVVVAVGAVIAGATGAFLSDTETSSGNTFSAGAIDL
                     KIDNESYYNNAFNPGTSWELDDLDGTGHLFFNFLDLKPGDEGEDTISIHVDDNDAWAC
                     MDIALTANEDNGSTEPELDDEDPYTGDVGELAEAINFIWWKDDGDNVLEQGEEDESVI
                     EATLAGLNGFALPLADISGNALFGDEPLEGSQTYYIGKAWCFGSMTINPLPEGDGSPA
                     DRPEN"
BASE COUNT         3869 a         3082 c         3882 g         3338 t
ORIGIN      
        1 ggcggcgacg gcggcgaagg atggaacggc ggcatgggag gtgatggcgg catgggaggt
       61 gaaggcgaca acggtggcga cgctggtaac ggcggcgacg gcgcagaagg tggcgacggc
      121 ggtgatggag gaggcgatgg cctcatcaca accggcgacg caacctcgac tggccgcgtg
      181 gttaacattg ttaaccgcaa catgactcgc gttcgacgct agtctttctg aaggcggaga
      241 gcgcagggct ctccccttcg aatgtgcttt tcaggaaata aaaggcacat gcgagggttt
      301 gtatgaaatt tatacagaac acaatactat ttttttgcct attcggggtt ttgggtactg
      361 cgcatgcgac aaccgtgcag aacgaaattt ctgtttctgc ttcaaccgga gggaatactg
      421 ccggccagga aacccagggg aaaagcgagg taagcgtgtt tatcgaaaca acagtggacg
      481 gcgaggtagt ggagcatatc gaagaacaca aagaagatac ccagggcaat ccggtggaaa
      541 ttcgaaagga aaccagctac caggacggta aagtgcaggt gcaaacccag agcgcagttg
      601 aagtattgcc agaagataat gctaataaaa atgttgaaga ggtcgaaccc gagaatgaat
      661 caaagccgct gtttgttgct ttgctagact tcattcgaga tatattcagt aatttatttt
      721 ttgcatgaaa aggtttaact ttcacgtcac atttgtggta gcatgcttgc tctttgtagc
      781 gcttaattta tataccgttg attttcccgt attcgcgcag gatgcaggta gtgaagtagt
      841 gcaggaagat ggaaaagatg gcaaagacgg cgaaaaaggg caagagggcg aagacggtca
      901 ggaggggcag gatggtgagc aggcgactga tggacaagaa ggcacagacg gtaaagaggg
      961 cgaagaagga caagcaggag agccgcaaga tgatccgcct gcagaggatg gtgagaacgg
     1021 cgacccagcc tcggatggcg aaagtggcga ctctggagaa cccggagaca acggcgacaa
     1081 tggcggcgaa gaagaaaacg gcgaagacgg aggcgatgcc accgacggtg aagacgccac
     1141 agatggagaa gatgggggag atggagagta tggcgagaac ggtgaagatg cgaccggagg
     1201 cgaagaagac tcccagggag aggcgcaagt ggaaacgggc gacgcaactt ctgccggcca
     1261 aatccaaaac acggcaaact caaactcggt aaattccaaa gagcctggca gtgaagaact
     1321 tcctttctgc tccgacaaaa caagaaaaga tgaaggaaag atagaagacg aaggcaaagg
     1381 aagcaaagaa gactcgcaag acgaagaggc accttactgc gttgaagaaa attcttctgg
     1441 cggcagcggg ggcgaaatgg aagtggaaaa caccaacgca gcagacattc aaaatggtgc
     1501 cgaggcggga gctgaaaccg gagaaaacga ggcgagcaac aatgccggag acgccaccat
     1561 cgacacgggc gacgctacgg ccgtactcga tttgttcaac cttataaaca ccaacgttat
     1621 taattcgcag gggcttttcg tgctgctttc caatttgcag cagcagcttc tcgatatcga
     1681 cttgcgagac tggatgccct atttgcagga ttccacagac tgcgatcctg cgcagtgcgt
     1741 acgattgcag gagttgctgg taaaaaacca gaatattgca aacattgtaa atgaattggc
     1801 tgtatatgct ggtacaggag gcaacgaggc aaacgataac accggagacg catcgattgt
     1861 cacaggagac gcgtatgctg cggccaacat cataaatatc gccaacacca acattatcaa
     1921 ctccaattat ctcttattta ttgcaaacaa ctttggaaat ttctttggag acattgtatt
     1981 tccaaactct tcttcgttcc tgcaggcgtt aatggcctct cccagtcagg tgtacagcga
     2041 agtggaaatc gacaacacca acaacgccag cgtatccaat aatgtcggcg tggatgcaca
     2101 aaccggggga aacgaagcca atggaaatgg cacatcgctt gtgcaaacgg gagacgcgaa
     2161 tgccgtggca aacgtgttta accagataaa catgaatttg tttggcggca actcgtttgt
     2221 agctttggta agggtttcgg ggaattggtc gggcgacata ttcgggctgc cggcgggaat
     2281 ttcctgggtg caaacgccgg gcggaattat gctgtactct gacgatttgt cccccttagg
     2341 gggatctccc gaagggggag aacttggaac agcagcggaa gattcttctt ctgaaaccgg
     2401 cgaccaaccc ggagaagaag gcaaaagtgg aaaggggagc aaaaaagatc attccgagct
     2461 gtcggtagaa aacgaaaaca acgcctcgat taccaacaac gtgcaggtat acgcgctcac
     2521 tggcgaaaac aaagcttccg agaacggtgg gaacgccagt atccaaacag gggacgctct
     2581 tgcggttgca aacatcgtaa atattgcaaa caccaacgtt atcgggaaaa actggctatt
     2641 tgccatattc aacattttgg gagatttcga cggcaacatt agtttcggaa ggcccgattt
     2701 gtggattgga gccagagcag aaattcctca cgatcctttg cttccgggcg acgaattcac
     2761 gtattacctt acggtagcaa acaacgggga tgcgcctgca aatcaggtaa ggattaccaa
     2821 tgtgttcgac gacggtttcc ttgctttcgg atcttccctg gacaacacgg gtctcgatgc
     2881 aaacaaggtt ttctgggatt tgggtacgct gaaccccgga gaaggcagag aagtttcgtt
     2941 tacggcttct attcgcaccg atattccata tggcgtgcat gaatttatga atacgagcgc
     3001 cattggggca aaagaatccg acgacaacaa tcaggacaac accgacagcg tagagcttgc
     3061 ggtatttcac gaagaaccgg cgcctccatc gggtggtggc gggaatccgc ctaacaacga
     3121 ttcctcagac ggcggagacg gcgacaataa cggaaacgac aacggaggga acaattccga
     3181 tggagacgga ggaaatgaca cagacagcgg aaactcggat tcgaatacgg attcttcttc
     3241 gcaaaacaac gtttctccca cgcaacggac aaacgcaaat ccaagcgatt cgaccaaggc
     3301 ccctaatctg catgtgcaga aaaactttgt gggcgaggga gtggtcgtgc caggtgactc
     3361 ggtagactac accattgtcg ttaccaacac caacggaggt tcggcatacg acgcaattct
     3421 tgtggatacc ttggaggatc aagatgggaa tgtggtaaac gaggagatat gggatttggg
     3481 cgaggtattc cccgacgaag aaataactct tacctacaca gtcgtgttcg acgacgaaac
     3541 agaggctggg ttgtacacaa acagagcgca ggcggtggct tacgaaggtt ctgtggacgg
     3601 taaagaagta agttcaaaca ttgcgagcgt tggcattctg gtcgaatggg agagtcctgc
     3661 gccccaagag agcattcttt ctttggctaa ttcgttccag caaggcagcg gctttgccct
     3721 ggcttctccg cagccttccg gcgcagggga acccgggcag gaagcccccg cgcaggcaaa
     3781 cgaagacccc tttgtgttct ctaacgagga aaccaggcca ttttacgctt ctttggcagg
     3841 tctgcttaac gatttgagcg gctggcttat agctttagca agcgccgttg ctctcttttt
     3901 gctggtattt ctcttgaaga gaagaaggtc ccagcaggca tagaagggga aaagtccatt
     3961 tgcctgcaaa agaaacttct gctaccataa aatcatatga aaactctttc aaagcagttg
     4021 agcatttttt gtgtcatttc gctggtcgta acttcaggcg ttttcttccc tgtagtgaat
     4081 gcgcaaggag aattgcgcga ggccagagag gtacgtgaag ttcgagccgc aagagaagaa
     4141 ggagtggtag tggtgaaatt tcatgggtcg gatcgtgaac agcgtattag gttgagtgcc
     4201 ggcgaaaccg tggaggcggc tttgcaaagg tatcgccagc gccaagacgt ggcgtatgct
     4261 gaaccaaact atatagccga ggcctttggc gtgccaaacg atccttatta ctcgtaccaa
     4321 tggcacctgt cgcagattgg tttggaagca gcatgggata tttcaactgg ttctggtgca
     4381 gtggtggcgg taattgacac gggagttgca cagctttccg atcttgcagg cacttctttt
     4441 gtacaagggt acgattttgt gaacaacgat tcaaacccca ccgacgacaa cggccatggc
     4501 actcacgtgg caggcacgat tgcgcagtct accaataact ctttgggagt ggcgggtgtg
     4561 gcctatggag caacactcat gcccataaaa gtgcttaaca aaagcgggag tggcacctat
     4621 gccgatattg ccgacgccat acgctatgcc acagataacg gcgccagggt aattaacatg
     4681 tctttgggag gaccttcacc tgcaacatat ctgcaagatg cactgcaata cgcatacggc
     4741 aaaggcgtta cgatagtggc agccgcagga aacgacgggc aaaacagcat ttcataccct
     4801 gctgcttaca atgattacgt aattgcagta ggagcaacac gtttcgataa agccagggct
     4861 ccgtattcaa attatgggtc aggcctcgat gtggtggctc ccggagggga cacctcggtt
     4921 gaccagaacg gcgatggtta tggcgacggc gtgctccagc aaacatttag cagcactccg
     4981 aacagtcccg gctactattt ctaccagggg acttctatgg caactccgca cgtggcagga
     5041 gtggcggcat tggtgatttc aaatggaaat gcgcaaactc ccgcagacgt ccgagaagcg
     5101 ctgcaatcaa gcgcagaaga cctggggagc gcaggctggg attcttcgta tggccatggg
     5161 cttatcaacg cggcctcggc gcttgcatgg agtagcagtg gctctcctac gccaccaccg
     5221 tctccgtccc caccacctcc tgccgaaata gaagtgtttt cagaaagctt tgagcagggt
     5281 ttgggaagct ggacacagga ttcccaaaac gactggttcc gttcttcgca acgcgatatg
     5341 gagggtcgtt attcagcgga agtagacggt tcggcttcta acgcctccat aatatcttct
     5401 gcgatagatt tgcagggaaa aaccgctgca aaaatagctt tttcctggta tatcgaaaga
     5461 ggtcttgatt cgggagaata tcttgcgttc gacatttcaa ccgatggggg aaccacttgg
     5521 gtggaaaagg cgcgtttgcg cggcaaccag gatcccgaaa actcatggcg ccaggttcaa
     5581 accgatttat cgggtatctc aaaccttaag ctgcgctttc gtggaactat gtctggctcc
     5641 gccgaagacg ccaatgtgga caacgtaaaa gttaccgctt actaagggtt gtggtacaat
     5701 aggtgaatga tcgaaggaat tgttttggga accattcagg gaatcaccga atggctgccc
     5761 attagttcgg aagccgccat cattttggta aaaacgcgct tttttcccaa tggggaaggt
     5821 ttagccgacc tcattgggct tgccgtattt ttacatttgg gcacgttttt ggctgctgcc
     5881 tggtatttca gagacgacgt aataatgctt gccagaagcc ttttcctgcc gcacaagctg
     5941 ttccaaagag aagaacgttt tcttatactc cgcttcctta ttgtttccac atttataagc
     6001 ggaattcttg gctatttttt gatagaaatg gtagtgcccc gggccgagga gcacattgag
     6061 cttggagcta aagcagttac tcttggcatt ggcatattgc tggttattac cggatttttc
     6121 ttgctgcagg caaaaggcaa aaaaggccag cggggggtaa tccatttgcg cgacagagac
     6181 ggcgttttgc tcgggtttgt gcaagggttt accgctttgc cagggttttc gcgttccggg
     6241 cttacgattt ctacgctttt gctccgtaag ttcgaagaaa tcagcgcatt acgcttgagc
     6301 tttttaatga gtttgcctgc ggtgctcgga ggaaacattc ttttaaatgc aagaggtttt
     6361 gagataactc ttacagccat atttgggctt atattttcgt ttttgttcgg catactcact
     6421 atgcacttgc tgtttaaagt tgcaaaaaaa gtaaatttcg gctggtttgt tttgatattc
     6481 gccgcccttg tgattgcctc ggtttttgtg tagtattatt cgtaaaacag caggagggat
     6541 gccatgccag aacctatgaa ggcatttatc gtaaggccgt gggtgcctat agaggaaacg
     6601 ccagcccaga ctaaagggga cagaaaacat cttaagcgca aaataggggc gcaagcgccg
     6661 cctcccgtcg cggatcccgt ggaggccgag aagacctcct ccgattctta cgccaagata
     6721 cgggccgact actatgcagg tcggcttaag cacttaaatc aggaaacgct tcaacaagtt
     6781 gtcgatgcgt ttctgtagta gcaggctttg ccaaggtctc tcctcaattg cgaggcgaga
     6841 ctttttatta tatggtgagt gtatggaaaa gtttttggta atttcgtcgc acccggatga
     6901 tttggaattt agctgcggag gcactgttgc caaactgcgc aaagacggaa agcaagtgga
     6961 atatcttatt gttgccgatg gatccaaagg tagccataaa gtggggttcg acggcaaaaa
     7021 actcgcccaa ttgcgccaga aagaacaaaa aacagcggca aagacgcttg gagtagaaaa
     7081 agtaaccttt cttggcgagg tagacggcgt tgtagaaaat accccccagc tccgcaaaaa
     7141 gctggtcaaa gaaattcgcc aggcaaaacc cgatgtggta ttaagtttcg acccctcgag
     7201 cttagacttt gaaaacgtgt atctggcgca tcgcgatcac cgcatggcgg cagaagccgc
     7261 attcgacgcc ttgtaccccg cggtgggtag cggcgccttt ttcccggaac tcctcaaaga
     7321 ggggataagg ccgcatcaag tcaaggaaat atggtttttt gccacctcaa ggccgaacaa
     7381 gtgggtggat attaccgcga ctattggctt aaaactccag gctctttttt gccacgaaag
     7441 ccaacttgaa gatgcgaaaa aatttgagga aataatccgg aaaagtgcag caaaagaagg
     7501 caaacaaaag aaaataaagt acgccgagtg ttttcgagtt atcgactggg gataactggc
     7561 ttgacgcttt attccgcttt aattaagctt aaagaaaggt tgtggatact aactatcaac
     7621 agtaatctct tttttcaatt tttctatggc aaccgaagga tactgcgtta agtgcaaagc
     7681 aaagcgcgaa atgacaaacg aacaaaagac cgagatgccg aaaaagggcg gcggcaccag
     7741 gcccgctatg aaaggaacct gtcctgtttg cggaaccgga atgttcagga ttcttccgat
     7801 gtcggaagcg taaagaagat attcaattat tgcctagccc ccacactttt gtaaaaggtg
     7861 tgggggtttt ggtttattga ctttttctat tttaaggtta aaatacggct agctattttg
     7921 tttaggggga gataacccat gataagccgt agaaaggcag gacaaatcac agactatgat
     7981 atctctgaga tttggaaaac ggttctcttt ggcatacctg ggctaggggg gttgccggat
     8041 ctcaatgcgg tggtaacagc catatacccc accgatactc ttgatgtcgc acgagaaaaa
     8101 atggcgtgct ctttctccac cacagcttta accctgtttc aggatctctt taaggagaga
     8161 gtcgacttaa atgaatattc tggtacgaca cgcgactttg tcaaaaagct gcaaggcaaa
     8221 actcatagct ttgaggatgt cgaggttgcg ctagaaaagt tcgcaacttc ccaaggagta
     8281 ccctttaaca atcataggca tgtgcctacg ttgtactgga agctcccatc cgcgattcct
     8341 aagtagatcc gtggcgaatc ccagtcgcca ctttttattt tacattatgg tagaatgacg
     8401 gaatgaaaaa ggtggggtat ttgggaataa tcgggttggc gctggcaaat atcgtgatat
     8461 ggccgtacgt ggtggagggg agtaatttgc gggttacgtt ttttgacgtg gggcagggcg
     8521 acgccatatt tattcaaacg ccgcaaggcc accaaatgct tatagacggc gggccagacc
     8581 aaaaagttgt ggaaaaagta gccaaagtcc tgcctttttg ggataaaagc cttgacttgg
     8641 tagttcttac gcatgccgat gccgaccata ttaccggctt agtaagcgta ttggaacagt
     8701 atgatgtggg aaatgtactc tggacgggtg tggaaaaaga aaccaatatc tttgctgcct
     8761 gggagcagga gttagcacaa gaaagggcaa atatttggac agcagacatt ctgcaaacaa
     8821 ttctatggtc gaaagatagg tcagccaggt tcgatgtggt atatgcggca aaagaagcaa
     8881 aagaaataaa cgatacttcg gttataacaa agctggtatt tggcgaaacc tcgtttctct
     8941 ttccgggcga catttccaaa aatgtggagc agcggctttt gcaggaaaaa ctcgctatag
     9001 atgctgatat tctgaagatt cctcaccatg gcagtaaatc ctcgagttca gaggcgtttc
     9061 tggccgctgt aagcccttat atggccgtta ttcaggtggg cgggcaaaac agctatggcc
     9121 atccggcttt cgaggtatta gagcggcttt ttgccatggg aacaacaacc ttgcgcaccg
     9181 atcaaaatgg tgatattgta attgaatatg actcatccaa aggaagaacg aacattagta
     9241 attattaaac cagacggcat tcagcgcagc ctggtaggag aaattattaa gcgctatgaa
     9301 cgcgtagggt taaagttggt gggcatgaaa atgcttgtgc cacaggaaaa caaggtagaa
     9361 cagcactatt tgcttgatcc tgaatggaag cgcaaagttg gcgaaaaggc aatcgaaagc
     9421 tatcagaaaa agggcgtgga acctccttcg aaaaatccta tcgaagtggg cgaccgtgtg
     9481 gttgccggcc ttaaaaaata cctcactgca ggcccggtag tggctatggt gtgggagggt
     9541 gcgcatgctg tggaaattgt gcgcaaatta accggtggca ccgagcctcg aagctccgat
     9601 gtggggacca ttcgtggcga ctttgtgctg gactcttacc aaatgggaga tgccgacaac
     9661 agagccgtac ggaaccttat tcatgcttcg ggttccgtgc aggaagccga ggcagagatt
     9721 gcccactggt ttgccaaaga ggaactcgtg aactacaaaa tcgtgcagga aaaaatcctg
     9781 tatgatgtaa accttgacgg catcctggag tagcgtggta taatagagtt gcccaggcta
     9841 tccttcgaat aagatccgca ttacgttttt tatgggagcc ggatatgctt gtgccccgtg
     9901 gggtacaact tcggcaccca cctggattat tccgggtgtc ttattcagag ggagctatgg
     9961 gcacgcaaaa aacgacatcg tgcctgatgg cgttttttgt tttataatgg aattatgaac
    10021 ccgatattca aacatatcca gaacaggtcg cttgcgcatg tattgcggtt taacttaaag
    10081 ccgcagcatt tcgaagaaag cgtggccgac cattcgtatt ttgtggccta cattgtgtct
    10141 gttttgtgct atttgttgga gcggcaaggg gagcaggtag acgctaaaaa agcgcttgaa
    10201 atggcgcttg tgcacgatat ggaagagata ttttcgggcg acattgtcac gcctcttaag
    10261 cattactcct cggaggtgga agaggcaatt accaaagtaa acaaggaaat gattccagag
    10321 gtgttttccg gcctcccaga agagctttct gcccatttta ttagcttatg gaccgaggag
    10381 ggccaggggc aaaccatgga ggctcaagtg gtaaaagttg ccgacaagct gtcattgctg
    10441 tctaaatgcg ccgaagaagt gcatgtgggc aacgagtttt tccaggaaat ttacgagaag
    10501 ggcatgcggt ttttggagga atacgacaaa ccctggtggc agaaaattaa ggcgcagata
    10561 cttccttaat tcgggcggct tcggcttcta ccacagggcc gtcgtgctct gcggcatggg
    10621 cgtgagtgcc cccataactg ggccttttct ggtctaagtg aaggttgagc ctggcgctcc
    10681 cgttttctgt tttggcgtaa acatgaaatt ttggataggt gtttcctcca ggacgttgaa
    10741 acgcaggctc ttcagaagat tgggaaaatc caggagcata ccctgcttgc cgcataagtg
    10801 tgtgtatatt tttccccgat agggggattt ggaaccgcat atcaattcca agcatatcgg
    10861 ataaaagcat ttttcgcaag aaattttggc attttttatg ggtttttggt atactatggg
    10921 tatatgcatc aaaaaatccg caattttcgt tacccgatag ggggccagca ggggaaagtc
    10981 gttccaatgg tactcatttt tgtcacactt ttggttgcgg gggccggagc tgttatgctg
    11041 ctcaaaaccc gcaatacggt aatcgtgata aacgaagcag ccacagcgcc ccaggaaagg
    11101 gttgttaccg aaagtcccca gccccaagca gaagccccgg agccggttcc tcaggtgcgc
    11161 atttcgcaaa cctcggtggg gtacttgaat gtgcgaaaat cgggatctgc gcaggctgaa
    11221 aaaatcggcg aagtgaaacc gggtgaagtg tacgagttca ccgaagtggt aaacaactgg
    11281 tatcatattg tgcacccaac tcttggggaa gccgcctggg tttccggcca gtatgttaca
    11341 gaggtagaag ccgaggcagg gaacagcaac attttctccc cgccctcgga aatttgaccc
    11401 cgtagtacgg ggttgacaag ccataaaaga cttctattat aatagtatag ggtgctatcc
    11461 gaaaaatata caggagggca gagtatggaa tacaggtcag ggggtatggt tctttgcacc
    11521 gagttgctcg gtgcgcatgg cggcactgaa tatgtgctgg cggaaatgca cccggaaaac
    11581 gataagcacg atcggattgc ggtacatttt accggtatgt gcaacatatt ggtaataacg
    11641 ccaagatata cccagataaa gtataacttg aactggcgtt tctggaatcg gcatgtacct
    11701 gcgcacctcg cagacgaccc atgcgacctt tttaagtgga tgttaggcct gtggctaacc
    11761 tttaaaacag gcctcgtgcc taatgggcta gggccagtta gatcactgcc ttaaaccaaa
    11821 tgtcctacta ggacaaagca atgtcccaac tcaaccatgg ttgggacatt ttatttcggc
    11881 gaaaactgcg cctacctttt ccccagaact ggggaaaaag gctgtggata attagtccta
    11941 ttgacgtgtt gacatgacat tatggctgtc ataaggtagc attacggagg agtttggaat
    12001 ccgctttttc tttttgttgc tggcattggt tttaccgctg ttgtggcggg ttggtaacgg
    12061 agaaaaggcg gattctgaac tctacatgtg tgttcggaac ataaaggtcg aactcgtaga
    12121 acattaaaaa ttaatgtcat agaacctatg aaaaaaatct taatgagttt aggtgtcatt
    12181 gttgctgtgg gggcaattgt tgcaggtgca acaggtgctt tttttagcga cagtgaaacc
    12241 tcggtaggca acacgtttac cgccggagca attgatttag gaatcgataa cgagagttac
    12301 tacaacggag tactaaacga cggtacgacg tgggatttga gttttgacct tgatgagtgc
    12361 gaaatccgta atccagagta taattccgat acagatcaag acggtgatgg catagacgcg
    12421 gatggagacc cagaattctt cccatgcctc ttctttaatt tcgacgactt aaagccggga
    12481 gactacggtg aggataccat tagcgtccac ataacaaaca acgaagcatg gctttgtgca
    12541 gatgtaacat tgacaaagaa tgatgatatg tcttgcacag aaccagaaaa cgatgttgat
    12601 gcggaaaacg gcgcttgtgt ggatcctggg ccaggccttg gcgatcttgc gcaggaaatt
    12661 gatttccttt ggtgggcaga cgatggcgat aacgtacttg aagacgacga aacgccgctt
    12721 ccagcaggaa aacttgggag ccttggtgta ggtaacaccg cgacagttcc tcttgcggat
    12781 tcaaatggaa atatttggac cggcaatcca gatgatccaa ttttgaccga tgaagaggac
    12841 gaaattctgt acattgggaa agcatggtgc tttggagata taactccagc gccagtccca
    12901 caagacggtt ttggtgatca aatgacacca gccggtgata acgaaggtgc aggtaatgtg
    12961 ggtaacggaa ctcttgggca accatctgat ggcggatacg cttgtgatgg cgaaggagtg
    13021 gggaacgaac cgcagactga ccaggcaatg ctcaatgttt cattccgagc agtacaggcg
    13081 cgacacaacg atgacttcca gtgcgtacca cagcaacaag aagagccagc tcaacttacg
    13141 gttattaaag aggttattcc tagtactgca ttcgatcaag acttgggcgt aggtgacttt
    13201 gacctgtttg ttgacgcaac tgcagttgta agtggagtgg ctaatgtctt tgggacagga
    13261 gcgcatgttg taagtgagac tgctgtggct gggtatagct ctgccattag tggagactgt
    13321 gatgctgcgg gaaatgttac tctgaatccg ggcgacaaca aaacttgtac gattacgaac
    13381 acagaagtgg ccgatgcgtc aacactatag tctgatgcga ctgtatttct ggggcggggg
    13441 cttggggaag ggtctccgct ccggaaaaca aaagtacgag aaataataag taacctactg
    13501 aaatgaaaaa gattttattc agcttggctg ttgtggtagc tgtaggcgct gtgatcgccg
    13561 gcgcgacagg agcattcttg tctgacacgg aaacttccag tgggaatacg tttagtgccg
    13621 gcgctatcga cttgaaaatc gataacgaaa gctactacaa caatgcgttt aatccaggaa
    13681 ccagttggga actggacgac ttggatggaa cggggcatct tttcttcaac ttcctcgatt
    13741 tgaagccggg tgacgaagga gaagatacca tttccattca cgtggacgac aacgatgcct
    13801 gggcgtgcat ggatattgca ctcacagcca acgaagacaa tggttccaca gagccagagc
    13861 tcgacgacga agacccttat actggtgatg ttggtgagct tgcagaagcc attaacttta
    13921 tttggtggaa ggatgacggc gacaacgtgt tggagcaagg tgaagaagac gaaagtgtca
    13981 ttgaggccac tctcgcaggg ctaaacggat ttgcgctgcc cttggcagac atttccggaa
    14041 atgcattgtt tggcgacgag ccattggaag gcagccaaac atactacatt ggcaaggcat
    14101 ggtgctttgg ctccatgacc ataaatccgt tgccagaggg tgacggcagc cctgccgacc
    14161 gacccgaaaa c
//
LOCUS       DASRUP010000027         8607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1426794, whole genome shotgun sequence.
ACCESSION   DASRUP010000027 DASRUP010000000
VERSION     DASRUP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426794"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFE94_01175"
     CDS             <1..477
                     /locus_tag="VFE94_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZX49746.1"
                     /translation="GQQLSNKAAIPIEQRFIALFPRKRVSAKHLLNLSEEKIRGAGTS
                     WAKVRSLKDLAEKTQSREIAFGKFLQMQDEEVIAELTKIKGIGRWTAEMFLIFTLARP
                     DVFSHGDLGLRRAVERLYSLEDPEQKAVEAIAIRWSPFRSYASRALWHTLDGEQAP"
     gene            842..1285
                     /locus_tag="VFE94_01180"
     CDS             842..1285
                     /locus_tag="VFE94_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZX49747.1"
                     /translation="MTNKERHKIVPAAYIILRDGNKILLQRRLNTGHYDGSYGFVSGH
                     VEENESYSSGIIREALEEAGFKLKPEYIRFAHFMHRKGEDSERADVFFVAGRWDGEVQ
                     NMEPHKCDDLSWFERDNLPENLIPHLRNVLEYIDQNIMYSEHGWE"
     gene            1287..1925
                     /locus_tag="VFE94_01185"
     CDS             1287..1925
                     /locus_tag="VFE94_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HZX49748.1"
                     /translation="MAIFLIILAIILALPFLVIFGFLHIVTESFEAFGLPPGVVIAIL
                     LAMLVGSVANIPLGRRRLVETQDVRFFGLIRQKRYMPQGLSINVGGALIPLFLALYLL
                     QFAPLKETLTACAIMIVICFALARFMPGKGIGLPTLLPALFATLIALILAGDQAAPVA
                     FVSGVFGVIIGADLLHLPRVMRQGQGVMSIGGAGVFDGIFIIAIVAAFLAGL"
     gene            1969..3309
                     /gene="secD"
                     /locus_tag="VFE94_01190"
     CDS             1969..3309
                     /gene="secD"
                     /locus_tag="VFE94_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZX49749.1"
                     /translation="MKRWKTVAVVALIVLAALLAGFFDFPDYINPWLQKVPGGVQVPT
                     IPFRLGLDLQGGVHLVYQADLSKVDPLEYDAAMQGLRDVIERRVNFFGVSEPLVQTEG
                     RGDERRLIVEIAGVINSQEAIDLIGRAPLLEFREPKDNYEEILASNDKVFETGEGELQ
                     EPFQETGLNGSLLQRADVGFEQFAQEPVINLTFNSEGARLFEEITGRHIGRPVAIFLD
                     NQLLSEPIVQSQIAGGQAQITGSYTVEEAQQLVRDLNAGAVPVSIELLSQQQVGPTLG
                     SVSLQQSLRAGMAGLLLVIVFMTFMYRLPGVLASIALVFYGIFLLFIFKVLGFTFTLA
                     GIAGLILSIGMAVDANILIFSRMREELQEGKSFAQSVEEGFKRAWPAIRDGNITTLLI
                     ALILFWFGTSFVQGFALALSIGILVSMFSAVVVTKSFLRLFAGTPLEKVRRLWS"
     gene            3314..4207
                     /gene="secF"
                     /locus_tag="VFE94_01195"
     CDS             3314..4207
                     /gene="secF"
                     /locus_tag="VFE94_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZX49750.1"
                     /translation="MNILRLGKIFLALSVVLVGASIVSLAWFGLELGIEFTGGSLLEV
                     RYSQDRPSNAQIQEWLAPLGVQVSSVQPVGANNVLLRMQDLPEEKHQEVLSALEQGRP
                     QAVVDEQRFESIGPVIGRELTEKSLTLTLVSLLAITLYVTFAFRKVRKPARSWQWSLV
                     ALLALVHDVVIPLGTVAFLGHFQGMQITIPVMVALLTIVGYSINDTVVIFDRIRENLL
                     KKTGDSFAHTVAKSLRQTFSRSFNTSFTTLLAVVAIYFFGGATLQPFALTLIVGMVAG
                     TWSSLFLAPVILVKWAGKGAS"
     gene            4249..5292
                     /locus_tag="VFE94_01200"
     CDS             4249..5292
                     /locus_tag="VFE94_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZX49751.1"
                     /translation="MQFPYQKISITLVSGLPPRTKDVLVRRFGLQEEEPQTLEAIGQS
                     HGITRERVRQIVEDGIRQVKEAAKENREQEKLEAIFELLSDILKDSGYLKRQDLLVEA
                     VQAQKAGNHVVFLLHLGDQFYKHKETEYVHPFWASRKEVVEASPSVLDKILHFLQERK
                     EALHFDELRKAHGEIETEKLNPRTLASLLEVSKLITQAYNGKWGLREWPEVYPKGMRD
                     KAYVVLKNATKPLHFIDVTQLIEQLQESLVDQKKKSVLPQTVHNELIKDPRFILVGRG
                     TYGLSEWGYKPGTVKDVIREILKENGEAMDKEKIIQKTLAQRQVKESTILLNLQDKRA
                     FLRDQGGNYHLVS"
     gene            5347..6675
                     /locus_tag="VFE94_01205"
     CDS             5347..6675
                     /locus_tag="VFE94_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49752.1"
                     /translation="MADAVAQILFPFIQFLWVFVKTWWWVFLPFLLLPHAKYYWQWWR
                     MYTFDVTVRRIVLEVKFPPDVQKPIKAMESVFAVWWQIHDPPNFREKWIDGEYQLGFS
                     IEMVSTEGAPHFYIRLPEESRRIVEAGLYSQFPDAELIEVEDYTRSVPRNVPNKDWKM
                     WGATLKPGKPDIYPFRTYRKFFEETATEIKEETRVDPIAALIEGMAKLGEGEHLWIQF
                     LLSPRAPDETPFVEEGKKEVEKLVHRIPSSGKPATIPVTEDLRAVSATLFTGQPVEPR
                     LIGGVEEEQGLLAPELRLTTGEREVVGAIEEKISKQIFVTTLRFIYLARTEKYLGVTK
                     VLPFTYFQQFNTANLNFMRAIQTTKVYTVRSFFLDERRRYIRRRRLFRVYLSRDNFSW
                     PRSGGTFRLNIEEMASFFHFPGRIAFPSATVPRVEIRKGEAPSQLPWAEE"
     gene            6682..8451
                     /locus_tag="VFE94_01210"
     CDS             6682..8451
                     /locus_tag="VFE94_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HZX49753.1"
                     /translation="MQQQATPINFFGITNFRNIQRKFGIKLDDRRRHFYAIGKTGMGK
                     TEMLKNMAVQDIKSGYGMGFVDPHGEAVEDLLDFVPKERVKDVVYFNPADTRFPLAFN
                     VMEQVDPEKRHMVASGLMSVFKKIWPDVWSARMEYILNNVILALLEYPGSTLLGINRM
                     LSDSDYRAEVIEKVHDPVVKTFWLQEYSRYTQRYEVEATAAIQNKVGQFISNPLIRNI
                     VGQTHSSVDMRELMDSKKILLVNISKGRIGEDNSRLLGGLLITKLQLAAMSRVDIPEE
                     ARNDFFLYIDEFQNFATESFVNILSEARKYRLCLILSHQYITQLDETVEDAVFGNVGT
                     LVTFRVGAEDAEVFEKEFSPEFVQADFVNLGKYTIYVKLMIDGIAGRPFSAQTLPPPP
                     RQPESCRSEIIEFSRDTYGTTQQEVEDRITKWSGVEELAGAPPPPPPAQPQVLYDARC
                     SNCGKDTKVVFPPDGKRPVYCKSCRKKLQQQRPAPPQPVPQPSLPPQVQTVEEAPEKG
                     ESVSLEELKSREPNPLYAQKKQPPRARAPQERKAVEREGLQAALRQALDKVQTKPQAA
                     KAAKEQTSQKGVLEPGQTIYFDQ"
     gene            8454..>8607
                     /gene="pgk"
                     /locus_tag="VFE94_01215"
     CDS             8454..>8607
                     /gene="pgk"
                     /locus_tag="VFE94_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZX49754.1"
                     /translation="MKTLEDLPIQEGSRVLVRLDVNVPITDGIIHDDFRIAESVPTIR
                     YLAQKKA"
BASE COUNT         2154 a         1940 c         2361 g         2152 t
ORIGIN      
        1 ggccaacagc tttccaacaa agctgcgatt cccattgagc aaagatttat tgccttgttc
       61 ccgcgaaaga gagtgagcgc gaagcatctg ttgaacttgt ccgaggagaa gatacggggc
      121 gcgggcactt catgggcaaa agtgcgctct ctgaaagatt tggcagagaa aacgcaaagc
      181 cgggagattg cgtttggcaa gtttttgcaa atgcaagatg aagaggtaat cgccgagctt
      241 accaaaatta agggcattgg ccgatggact gccgaaatgt ttttgatttt tacgttggcc
      301 aggcccgacg tgttttcgca cggggacttg ggtttgcgaa gggcggtgga gagactgtat
      361 agccttgaag accccgagca aaaagcggtg gaggcaattg ccattcgctg gtcgcctttt
      421 cgtagctatg ccagccgcgc cctgtggcac acactcgatg gggagcaagc gccttgacag
      481 ggtaaacgag aattgcaata atgctaccta taaaaggagg tgcagaatgc aacttcgtat
      541 atagcccagc tgggggcctc gtacctagcc gagcaagcta ccgggagtaa gagtggcaaa
      601 aaacctcgcg ttgatggtcc tgacgaagaa ctggcctacg gaatttagcc agctccaaac
      661 gcttgccgta catggtgtcc agaccgagct cggccaaaag ggagggggaa gaggatgata
      721 gatggtgtca agtctttctt tccttccccc aaccttaaaa acattgacga caactttcag
      781 aagaggttgt ttttgttttg ctagaattga aagaaaggcg ggattttgct ataagcaagg
      841 tatgacaaat aaagaaagac acaagattgt tccagcagca tacattatct taagagacgg
      901 aaacaagatt ttgctgcaga ggaggttgaa tacaggccat tatgatggta gttacggatt
      961 tgtctccggg cacgtggaag agaatgaatc ctatagcagt ggtattatta gagaggcttt
     1021 agaagaggca ggatttaagt tgaagccaga gtatattcgt tttgctcatt ttatgcatcg
     1081 gaaaggagaa gatagcgagc gggcagacgt gttttttgtt gcagggcgat gggacggcga
     1141 agtacagaat atggaaccgc ataagtgcga cgatttaagc tggtttgagc gagataatct
     1201 tcccgaaaac cttatcccgc atctccgtaa tgtgctcgaa tacatagacc aaaacatcat
     1261 gtatagcgag catggctggg aatagtatgg caattttttt gattattttg gcgatcattc
     1321 tggcgttgcc ctttctggtg atttttgggt ttcttcatat cgtaaccgag agctttgagg
     1381 cgtttggatt gccgcccggc gtggttattg ccattctttt ggcaatgctg gttggttctg
     1441 tggcgaatat tccattaggg aggcggaggc tggtggaaac gcaggatgta cggttctttg
     1501 gattgattcg ccaaaaacgg tatatgccac aagggctctc cataaacgtg ggaggggcgc
     1561 ttataccttt gtttcttgct ttgtacctgc tgcaattcgc tcctctcaaa gaaacgctta
     1621 cggcttgcgc gattatgatt gttatttgtt ttgcgcttgc gaggtttatg ccgggcaagg
     1681 ggataggctt gcctacgctt ttgcctgccc tttttgccac gttgattgct ttgatcttgg
     1741 ctggcgacca ggcggctccc gtggcatttg tttcgggtgt gtttggcgtg attattgggg
     1801 cagacttgct gcatttgccg cgggtaatgc gccaggggca gggagtaatg tctattgggg
     1861 gagccggcgt gttcgacggc atttttatta tcgccattgt cgccgcattt ttggctgggt
     1921 tatagcgcga cttgaggttt tgtcaacaat atgatagagt gggaggcaat gaaaagatgg
     1981 aagaccgttg cagttgtggc gcttattgtt ctggcggcat tgctcgccgg attttttgac
     2041 tttccagatt acataaaccc atggttgcaa aaggtgccag ggggcgtgca agtgcctacc
     2101 attccttttc gcctcggcct cgatttgcag ggaggcgttc atttggtata ccaggcagac
     2161 ctttccaagg tggatccttt ggagtacgac gctgctatgc agggtttacg cgacgttatt
     2221 gagaggcgcg taaacttttt tggggtatct gaacctttgg tgcaaaccga aggtagggga
     2281 gacgaaaggc gccttatcgt agaaatcgca ggggttataa attctcaaga agcaatagac
     2341 ttaatcggca gggctccgct tttggaattc cgcgagccca aagacaacta cgaggagatt
     2401 ttggcgagca acgataaagt ttttgagaca ggtgagggcg agctgcaaga gcctttccag
     2461 gaaaccggcc taaacggaag cctgctccaa agagcagatg ttggttttga gcaatttgcg
     2521 caggaacccg ttatcaacct tacctttaac agcgaggggg ctcgcctctt tgaagaaatt
     2581 accggccgcc acatagggcg ccccgtagcc atttttctgg acaaccagtt gctttctgag
     2641 cctattgtgc agtcgcaaat tgcaggaggc caagcccaga ttacgggcag ctataccgtt
     2701 gaggaagccc agcaattggt tcgcgattta aatgcgggcg cggtacccgt gtctatcgaa
     2761 ttgctttccc aacagcaggt agggcctact ttgggcagtg tttccttaca gcagagcttg
     2821 cgggcaggca tggcagggct tttgctggtt atcgtgttta tgacttttat gtatcggctt
     2881 cccggagttt tagcgagtat cgccttggtg ttttacggca tattcctttt gtttattttt
     2941 aaggttcttg gatttacgtt taccctggcg ggaattgcgg gactcattct gtccatagga
     3001 atggcggtag acgccaatat ccttattttt tccaggatgc gtgaggagtt gcaggaagga
     3061 aaaagttttg ctcaaagcgt ggaagaaggt tttaaaaggg cgtggccggc cattcgggac
     3121 ggcaacatta ccacactttt aatagcgctt attctgtttt ggtttggcac gtcgtttgtg
     3181 caggggtttg cgttggcgct ttcgattggc attttggtaa gcatgttttc tgcggttgtg
     3241 gtaacaaaaa gttttttgcg gctctttgcc ggcactcctt tggaaaaggt gcgcaggctt
     3301 tggagttaac gctatgaata ttcttcgact gggaaaaatc tttttggctt tatcggtagt
     3361 tctggttggg gcaagtatag tgagtcttgc gtggtttggt ttggagctcg gtattgagtt
     3421 taccggcggc agtttgcttg aggttcgata ttcccaggat cgcccttcca atgcgcaaat
     3481 tcaggaatgg ttggctccct tgggagtgca ggtgagctcg gtgcagcccg taggggcaaa
     3541 caacgtgctc ttgcgcatgc aagatcttcc agaagaaaag caccaggagg tgctctctgc
     3601 gctggagcaa ggcaggcctc aagcagtcgt agatgagcag cgttttgagt ctatagggcc
     3661 ggttatcgga agagagctta ccgaaaagtc gcttacgctt acgctggttt ctttgctggc
     3721 aattaccttg tatgtgactt tcgcgttccg caaggtgcga aagccggccc gctcgtggca
     3781 gtggagcctg gtggcgctct tggcccttgt gcacgatgtt gttattccct tgggcacggt
     3841 tgcttttctt ggccatttcc agggcatgca aattaccatt cctgtcatgg tggcgctcct
     3901 taccatcgta ggatattcca ttaacgacac cgtggtgata ttcgaccgca tccgggaaaa
     3961 ccttttgaaa aaaacagggg atagttttgc tcatacggtg gccaaaagct tgcgccagac
     4021 gttttcgcgc tcgtttaaca cctcttttac cacgctgctg gcggtagtcg ccatttactt
     4081 ctttgggggc gccaccctgc agccttttgc tcttacgctt atcgtgggta tggtggcggg
     4141 cacctggtct tccctgtttt tggctccggt aatcctggtg aaatgggccg gaaagggggc
     4201 ctcttgacag gggttgacat attaacataa atttggttta atctataaat gcaattcccg
     4261 tatcaaaaaa tcagcataac cttagtttca ggtttgcccc cccgcaccaa ggacgttctg
     4321 gtgaggcgat ttgggctcca agaagaggaa ccccaaaccc ttgaggctat cggccagagc
     4381 cacgggatta caagggaacg ggtgcgccaa atcgtggaag acggcattcg ccaggtaaaa
     4441 gaggctgcca aagagaatcg cgagcaagaa aagcttgagg caatttttga gcttttgtcc
     4501 gacattctta aagactccgg gtatctcaag cgccaggatc ttttggttga agctgtccag
     4561 gcccaaaaag caggcaacca tgtggtgttc cttttgcacc tgggcgacca gttttacaag
     4621 cacaaggaaa ccgaatatgt gcatcctttc tgggcaagca ggaaagaagt ggtcgaagca
     4681 agtccttcgg tgttagacaa gattttacac ttcctgcagg aacgcaaaga agctctgcat
     4741 ttcgacgaat tgagaaaggc gcatggcgaa atcgagaccg aaaagttgaa ccctcgcaca
     4801 ctggcttcgc tactagaagt ttccaagctc attacgcaag cctacaacgg aaaatggggg
     4861 ttgcgagaat ggcctgaagt ataccctaag ggtatgcgcg acaaagcgta tgtggtgtta
     4921 aagaacgcca ccaagccctt gcattttatc gacgttaccc agttaatcga gcagctccag
     4981 gagtccttag tcgaccagaa gaaaaagtcg gtgttgcccc aaaccgtgca caacgagctt
     5041 atcaaagacc cgcgctttat tttggtggga aggggaactt acgggctttc tgaatgggga
     5101 tataagccgg gaaccgtaaa agatgtgatt cgcgagatcc tgaaagaaaa cggcgaggcg
     5161 atggacaagg aaaagattat ccaaaaaacg ctggcgcaac gccaggtgaa agaaagcacc
     5221 atcttgctga acttgcagga taaaagagca ttcctgcggg accagggggg gaactaccac
     5281 cttgtttcgt aaaaaagcca cgtatagtat aatagagaaa gactgaaacg tggctttttg
     5341 ttttctatgg cagacgctgt agctcaaatt ctctttccct ttattcagtt tctttgggtg
     5401 tttgtgaaaa catggtggtg ggtgtttttg ccttttttgc ttttgccgca cgcaaagtat
     5461 tattggcagt ggtggcgaat gtacacgttc gacgttaccg tgcggcgtat cgtgctggaa
     5521 gtcaaatttc ctcccgacgt gcaaaaaccc atcaaagcaa tggaaagcgt atttgcggtt
     5581 tggtggcaga tacacgatcc cccgaacttc agggaaaaat ggattgacgg ggaataccag
     5641 cttggttttt ccattgaaat ggtttcaacc gaaggggcgc cgcactttta cataaggctt
     5701 cccgaagaat cgcggagaat tgttgaagcc ggcctttact cccagtttcc cgacgccgag
     5761 ttaatcgaag tcgaggatta caccagatca gttccccgaa atgtgcccaa taaagactgg
     5821 aaaatgtggg gggccactct aaagcccggc aagcccgata tatatccctt tcgcacgtat
     5881 cgcaaatttt tcgaggaaac tgcgaccgaa ataaaagaag agacacgggt agaccctata
     5941 gccgcgctta tagagggaat ggcgaagctt ggcgagggag aacatttgtg gatacagttc
     6001 cttctttcgc cccgtgcccc cgacgaaact ccctttgtgg aggagggcaa aaaggaggtg
     6061 gagaagctcg tgcaccggat tccctcatcg ggcaagccgg caaccattcc cgttaccgaa
     6121 gatttacggg cggtaagcgc tacgcttttt accggccagc cagtagaacc caggctcata
     6181 ggcggagtgg aagaagagca gggtcttttg gctccggagc tccgcttaac taccggagaa
     6241 cgtgaagtgg ttggagctat cgaagaaaaa atatcaaagc agattttcgt aaccaccctt
     6301 cgctttattt atcttgcgag aaccgaaaag tatttgggcg ttaccaaagt gttgccgttt
     6361 acctactttc agcagtttaa tacggccaac ctcaacttta tgcgggccat acaaacgaca
     6421 aaggtatata cggtgcggtc ctttttcctc gacgagcgaa gaaggtatat ccggaggcgc
     6481 cgtttgttcc gtgtgtactt gtcgcgcgat aactttagct ggccgagatc aggaggaact
     6541 ttcaggctta atatcgagga gatggcgtcg ttcttccatt tcccgggaag gattgcattc
     6601 ccgtccgcta ctgttccgcg ggtggaaatc cgaaaggggg aagctccttc ccaacttccc
     6661 tgggcagaag agtaatacgt tatgcaacag caagcaaccc caatcaactt ttttggaata
     6721 accaacttcc ggaacataca gaggaagttt ggcattaagt tagacgaccg ccgccgccac
     6781 ttttatgcta taggaaaaac cggcatgggt aagaccgaaa tgttaaagaa catggcggtg
     6841 caggatatca aaagcggata cggtatggga tttgtagatc cccacggaga ggcggtggag
     6901 gatcttttgg acttcgtgcc gaaagaaaga gtaaaagacg tggtgtactt taatcctgca
     6961 gacaccaggt ttcctttggc attcaacgta atggagcagg tggacccgga aaagcgccac
     7021 atggtggctt cgggtttaat gtcggtgttc aaaaaaatct ggcccgatgt atggtctgcc
     7081 cgcatggaat acattttaaa caatgtgata ttggcgttgc tggaataccc cggatccacg
     7141 ctcttgggga ttaaccgcat gttgtcagat agcgactatc gggcagaggt aatcgaaaaa
     7201 gtgcatgacc ctgtggtgaa aacgttctgg ctgcaggagt actcaaggta cacccagcgc
     7261 tacgaagtgg aggcaacggc cgccattcaa aacaaagtag ggcagtttat ttcgaacccc
     7321 ttaatacgca atattgtggg ccagacgcat tcctccgtgg acatgcggga gctcatggac
     7381 tccaagaaaa tcctgcttgt gaacatttcc aaaggccgca ttggcgaaga caactcaagg
     7441 cttttggggg gacttttaat taccaagctt cagttagcgg ccatgtcgag ggtggatata
     7501 ccagaagagg ctcgaaacga ctttttcctg tacatcgacg aattccagaa ctttgccacg
     7561 gaaagctttg tgaacatttt gtctgaagcg agaaagtacc gtttatgctt aattttgagc
     7621 caccagtata ttacgcagct cgacgaaacc gtggaagatg ccgtgtttgg caacgtgggc
     7681 accctggtaa cattccgcgt gggcgccgaa gacgcggagg tgtttgaaaa agaattttct
     7741 ccagagttcg ttcaggcgga tttcgtaaac ctcggcaaat ataccattta cgtaaagctt
     7801 atgatcgacg gaatcgcagg ccggccgttt tctgcacaaa cgcttccgcc tccccccagg
     7861 cagcctgaat cctgccggtc agaaattatt gaattttcgc gcgacaccta cggaaccacc
     7921 cagcaggaag tggaagaccg tattaccaaa tggtctggag tggaagagtt ggctggcgcg
     7981 cctcctccgc cacctcctgc gcaaccccag gttttatacg acgcaagatg ttccaattgc
     8041 ggaaaagaca ccaaggtggt attccctcca gacggcaagc gccccgtgta ttgcaaatcc
     8101 tgccgcaaga aactgcagca gcagcgccca gccccgcctc aacccgtgcc gcagccttcc
     8161 ttgccgcccc aggttcagac cgtagaagaa gcgcctgaaa aaggagaatc ggtttccctg
     8221 gaggagttaa aaagcagaga accaaatccg ttgtacgcgc agaaaaaaca gcccccgcgc
     8281 gctcgtgcgc cacaggagag aaaagcagtg gaacgggaag ggctgcaggc tgctttgcgc
     8341 caggctttag acaaagtgca aaccaaaccg caggccgcaa aggcagccaa agaacaaacc
     8401 tcgcaaaagg gcgtacttga accaggccaa actatttact ttgatcaata gcaatgaaga
     8461 cgcttgagga tcttcctatc caagaagggt cgcgcgtgct tgtgcgcctc gacgtaaacg
     8521 tgccgattac agacggaata attcacgacg attttcgcat tgcagaaagc gtgccgacta
     8581 ttcggtattt ggcacaaaag aaggcca
//
LOCUS       DASRUP010000028         3949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_106248, whole genome shotgun sequence.
ACCESSION   DASRUP010000028 DASRUP010000000
VERSION     DASRUP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106248"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2253
                     /locus_tag="VFE94_01220"
     CDS             <1..2253
                     /locus_tag="VFE94_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49755.1"
                     /translation="YALVVLLPLWFLPVTQNVLGYQKQALLIVLVFAGFAAWLAKQVQ
                     EGELRVQKSWLLLAGAGVLFAAGISTIFSLWQYGSFWGWPLDVTDNFLSLFGFFLFAI
                     LLSHVVKDTKQLSYLLSGFVVSGTVATLYALAQMKGIAFLPFEFSGVKTFNTIGSTNS
                     VGIFAAFLLPLAWSLASFASRVWRGVLWAAAFLLFGSLLLLNFEAAWIVMITGLVVLL
                     GFDMWNARKREQFGRISLPMIFILVGFFFLVVNGVGIPGAPERQAEISPTWRGELAIV
                     QGMAGENPLSLAVGSGPGTFVFDYTKFRDASLNDTIFWSARFEKGAAEILDWLVTKGT
                     LGVFALLGMIGLGLFLGIRRLLTEEDSAALALGAGILASFAGVSLSFFLYPANFVFWF
                     TFWILASCLVFLTGKKMHVISFKRGPSYVGFAGSLGLLIVLMAEFGLFIVGAQKYYAE
                     VEYTRGIQTSRQENIEEAIAKVEHATELNASADVYWRDLGQLYLEQANQIVTNQTLPE
                     DVRRQQIEVAVDRATRAVQQATNAAPRNVANWNVRGLIYRNLIGALPEAEKFAVTSYE
                     QALALEPASPFPLTELGRVKILSSQAQEAIEYLEKAITLKSDYAPAHYLIAVAYDQMG
                     NQAEAIRKLEDAKLVTPGDTGLAFQLGVLYYRQQELGKARDEFERAKGVNLRYSNARY
                     MLGLVYDRLDRRQDAIAEFAAVAALNPDNQEVRTILSNLAAGRAALDGIGQPSTPPIQ
                     DNPEEIEQEE"
     gene            2262..>3949
                     /locus_tag="VFE94_01225"
     CDS             2262..>3949
                     /locus_tag="VFE94_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZX49756.1"
                     /translation="MPRKFLIAAIITIAVLALTGGTLALNGYFGGGGDINANDTTRGL
                     VGYWAFEEGSGLTAADSSDQGNNGTLTGVPVWTSGKAGNGGALDFDGSSDFVDVGAGP
                     GTVNTVAFWVNPTTTTEYPVDLNGTAYIWINAGTVTAQGFTSPTIYVDGAESSTVSAG
                     SWQYVAVTTGTSLNASDLDIGRVEGTGNHEGRIDEVRIYNRALSAAEIKFLYNHGGPV
                     IHLKMDEGSGSILFDATGNNNHGKVSGATFAAGQFGTALDFDGSGDAVSLSHFDSASE
                     SSDGAGDENITWSHTVGTGNDRVLVVGVYIRGLGNSPTVSGITYNGTALTKVDDETNG
                     AVRVEQWYLLSPDSGTHNIVASFSAASEGSRITGAVSLHGLVQAAPEATNSTNTSATS
                     VSVDVTTVTDGAIVVDAVGHRNETDQDITPGANQTERMELAPDTGNLMAMSTEVQPSA
                     GATTMSWSGDVSHSWAIIASAFRSSATFNTIADNFTVSAWVNPSSSIATKALIVKNDE
                     MRLVTDASGNLLCQIHNGSSWQTAATSSVALSLGSWQHVLCTYDQTDLKVYING"
BASE COUNT          905 a          889 c         1153 g         1002 t
ORIGIN      
        1 tacgcgctgg tcgtgctgct tcccttgtgg tttttgcctg ttacgcagaa cgtgctgggg
       61 tatcaaaagc aagccttgtt aatagttctt gtattcgccg gctttgccgc gtggctggca
      121 aagcaggtgc aagagggaga attgcgtgtt cagaagagct ggctccttct tgcgggagca
      181 ggcgtgcttt tcgccgcggg aatttccaca atattttcgc tttggcaata cggaagtttt
      241 tggggctggc cgctggatgt gacagataac ttcctttctc tttttgggtt ttttctgttt
      301 gccattcttc tttcgcacgt cgtgaaagac acaaagcaac tctcgtatct gctttccgga
      361 tttgtggttt cggggaccgt agctactctg tatgctttgg cgcaaatgaa gggaattgcg
      421 tttcttccgt ttgagttttc cggagtaaaa acatttaaca ctattggaag cacgaacagt
      481 gttggcattt tcgccgcgtt tttgctgccg ttggcttgga gtctggcttc ttttgcaagc
      541 cgggtatggc ggggagttct ttgggcagcc gcgtttctgc tctttggctc tcttttgctt
      601 cttaattttg aggccgcatg gattgttatg attacaggtt tggttgtgtt gctgggattc
      661 gacatgtgga acgcgcgcaa acgggaacag tttgggagga tctctctacc catgattttt
      721 atcctggtag gtttcttttt tcttgtggta aacggcgtag gaatcccggg agctcccgag
      781 aggcaggcag agatttcacc gacctggcgc ggagaacttg caattgtgca aggaatggca
      841 ggagaaaatc cgttgagtct tgcagtgggc tcggggccgg gaacctttgt gttcgactac
      901 accaaatttc gcgacgcctc gcttaacgat acgatatttt ggagcgcgcg ttttgaaaag
      961 ggcgccgccg aaatattaga ctggctggtt accaaaggaa ccctgggggt ctttgcgctt
     1021 ttaggaatga ttgggttagg cttatttttg gggataaggc gacttttgac ggaagaagac
     1081 agcgctgctc ttgctctcgg cgctggaata ctggcttcct ttgcgggggt ttccttgagc
     1141 ttctttctgt atcctgcaaa ttttgtattc tggtttactt tttggatact ggcctcttgc
     1201 ctggtgtttc tcacaggcaa aaaaatgcac gtaatctcgt ttaaacgagg tccttcttat
     1261 gtggggtttg ccgggtcgtt ggggcttctt attgtattaa tggccgagtt tgggttgttt
     1321 attgtgggag cgcaaaagta ctatgccgaa gtggagtaca ccaggggaat tcagacatct
     1381 cgccaggaga atatagaaga ggcgattgca aaagttgagc atgcaacaga gttgaatgct
     1441 tcggcggacg tatattggcg agaccttggg cagctctatt tggaacaggc aaatcaaatt
     1501 gttaccaacc aaactttgcc cgaggatgtg aggaggcagc aaatagaggt tgcggtggac
     1561 agggcaactc gggcagtgca gcaggcaaca aatgcggcgc cgcgcaatgt ggcaaactgg
     1621 aatgtgcgcg ggttaatata tcgcaattta atcggggcgc tgcccgaagc agaaaagttt
     1681 gccgtgacga gctacgaaca ggcgcttgct ttggaacccg cgtctccgtt tcctttgacc
     1741 gagctgggac gagtaaaaat tttaagttcg caagcacagg aagcaattga gtatctggaa
     1801 aaggctatta cgttgaaatc tgattatgct ccggcccatt acttgattgc agtggcctac
     1861 gaccagatgg gaaaccaggc agaagcaatt cggaagcttg aggatgcaaa actggtgacg
     1921 cctggcgata cgggactggc gtttcagctg ggcgttttgt actaccgtca gcaggagttg
     1981 ggaaaagcgc gtgacgaatt tgagcgggcc aagggcgtaa atctgcggta ttcgaacgcc
     2041 aggtatatgc tggggttggt gtacgaccgg ctggacagaa ggcaggacgc catagccgag
     2101 tttgccgcgg ttgccgcgct taatcccgac aaccaggaag tgcgtacgat tttgtcgaac
     2161 ctggcggcgg gccgagccgc gctcgatgga attggccagc ccagcacgcc gcctattcag
     2221 gataatccag aggaaattga acaggaagaa tgacagttat aatgcctcgt aaatttctca
     2281 tagccgccat aatcactatc gctgtcctcg cgcttacggg gggaactttg gcgttgaacg
     2341 gttatttcgg cggcggggga gatataaacg ccaacgacac cacccgcggc ttggtgggct
     2401 attgggcgtt cgaagaagga agcggattga ctgcggcaga tagttctgat cagggaaaca
     2461 acggcacgct tacgggtgtt cccgtgtgga cctcgggcaa agccggcaat ggaggcgctt
     2521 tggatttcga cggctcaagc gattttgtgg acgttggagc cggccctggc acagtaaaca
     2581 cagtggcctt ctgggtgaat cccacgacta ccacggaata tccggtggac ctcaacggca
     2641 cggcatacat ctggataaac gccggcaccg taacagccca gggttttacc agccctacta
     2701 tatatgttga cggggcggag tcttctacgg tgagcgcggg ttcctggcag tatgtggcag
     2761 taaccactgg aacgtccctt aacgcaagcg acctggatat tggaagagtt gagggaacag
     2821 gcaaccacga ggggagaatt gacgaggtac gcatatacaa ccgcgcgctg tccgccgcag
     2881 aaataaaatt tttgtataac cacggcgggc cggtgattca tttgaaaatg gatgaaggat
     2941 caggttcgat tttgtttgac gcaacaggga acaacaatca tggtaaagtt agcggtgcga
     3001 catttgccgc cgggcagttt ggcaccgccc tggatttcga cggttcaggg gacgccgtaa
     3061 gcctttccca tttcgattcg gcttcagagt cgagcgacgg cgcgggcgat gaaaatatta
     3121 catggtcgca tactgtgggg actggaaatg accgtgtttt ggtagtaggc gtatatattc
     3181 gggggctagg caacagtccc acggtaagcg gcattaccta caacgggacc gctttaacaa
     3241 aagtggacga cgaaacaaac ggcgcggtgc gggtagaaca gtggtatctt ttaagtcctg
     3301 acagcggtac gcataacatt gtggcctcat tttccgctgc ttccgagggc tccaggataa
     3361 caggggcggt gtcgctgcac gggcttgttc aggcagctcc cgaggctaca aattcgacaa
     3421 atacatcagc cacaagcgtg agcgtggatg taacaactgt aactgatggc gcgattgttg
     3481 ttgacgcggt gggacacaga aatgaaacag atcaagacat tacaccgggc gccaaccaaa
     3541 ccgagcgcat ggagcttgct cctgacacgg gtaaccttat ggccatgtct acagaggttc
     3601 agccctcggc gggagctaca accatgtcct ggtcaggcga cgtaagccac agctgggcga
     3661 ttatagcctc tgctttcaga tcatcggcga cttttaacac cattgctgat aattttaccg
     3721 tgagcgcgtg ggtaaatcca tcttcatcca tagccacgaa agccctaata gttaagaatg
     3781 acgaaatgcg tcttgttacg gacgcttcgg gaaatctgtt gtgccagatt cacaacggtt
     3841 cttcgtggca gactgccgcc accagttccg ttgctctttc tttgggcagt tggcagcatg
     3901 ttttgtgtac ttacgaccag acagacttaa aagtgtacat aaatggtat
//
LOCUS       DASRUP010000029         6288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1784942, whole genome shotgun sequence.
ACCESSION   DASRUP010000029 DASRUP010000000
VERSION     DASRUP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784942"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFE94_01230"
     CDS             complement(<1..281)
                     /locus_tag="VFE94_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZX49757.1"
                     /translation="MIKLASINIELDKHLDLVENFLKEEQPDVACLQEIYETDLQNLA
                     TKLGMTPLFGQMALMGRFPFGIGILSRVPFESVSRAYYFGDKETAPTHT"
     gene            338..853
                     /locus_tag="VFE94_01235"
     CDS             338..853
                     /locus_tag="VFE94_01235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZX49758.1"
                     /translation="MAKQIRTKRLLLRQYKKGDEESFLANINNPKVSRWTSGIPYPYS
                     KEDLETGQTILAKKQGKTEISFVITRENEAIGGVSLRGIRKGHKARIGFWLGEAYWGQ
                     GFMVEAIYAITKLGFKEFGLRRIEAKVFLPNKGSKRVLEKAGFRLEGLLEKEYLKNGK
                     FIDAYLFAKVK"
     gene            859..1401
                     /gene="msrA"
                     /locus_tag="VFE94_01240"
     CDS             859..1401
                     /gene="msrA"
                     /locus_tag="VFE94_01240"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZX49759.1"
                     /translation="MASVQFQTAVFGGGCFWCGEAVFQRLKGVQSVTSGYAGGSMENP
                     SYQQVSSGQTGHAEVVKVEYNPAVLSYDTLLSVFFATHDPTTMNRQGSDIGPQYRSAI
                     FYTSEEQKMEAEKFMQKLADDKVFKAPIVTELKPLEAFYEADASHKDYYNRNKEAPYC
                     QVVIDPKIQKLQEKYAHLLK"
     gene            1472..2203
                     /locus_tag="VFE94_01245"
     CDS             1472..2203
                     /locus_tag="VFE94_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49760.1"
                     /translation="MPTAAELKDLYYDRGEFDNCLVAIQNELVRHAQDDPERSELLAL
                     AGWCRYRKREYETARQLWQEAGQVRFAREGLAYLAAYIDKDDLALGELIRELGDSVNA
                     QNAFTIRARDQESTISHEQVMEGVLRFTGDTTVEMANLYHNAGRVFFHKARGREDLII
                     ALGLYDVARARYGIDRNWHHRGALHYWRSRVFEVLLDKRAALEAARDSLYCWTQQVIL
                     DPATESHKRNWENAVNRVRELVAAL"
     gene            2289..2894
                     /locus_tag="VFE94_01250"
     CDS             2289..2894
                     /locus_tag="VFE94_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HZX49761.1"
                     /translation="MKFLLATTNEGKIREYRHLLGKAGVEFVTLAEAGITDKPEETGA
                     TCAENAILKAKFYFEKSGGMPTLADDGGLEIDTLGGEPGVHSRRWPGHEATDGELVQY
                     TLERMEGIALEKRTARLTAVLAVTADGKNVQTFSGTLEGKIVEKPYNPIVAGYPFRSL
                     FQEMQTGRVLAEIPVEQLPNAHREQAIAKALPLLQQLAKNW"
     gene            2909..3271
                     /gene="msrB"
                     /locus_tag="VFE94_01255"
     CDS             2909..3271
                     /gene="msrB"
                     /locus_tag="VFE94_01255"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZX49762.1"
                     /translation="MDKLTPEQYRVMREKGTEAAFSGKYWDSHEKGMYKCASCGKELF
                     SSDTKFDSGTGWPSFTEPANLANIELKEDNSHGMKRTEVLCKNCGAHLGHVFNDGPAG
                     QGGKRYCINSVCLELEKE"
     gene            3268..4428
                     /locus_tag="VFE94_01260"
     CDS             3268..4428
                     /locus_tag="VFE94_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49763.1"
                     /translation="MKKTYLFLIIGASMLAGAGIFLAQGFFDNKEADALLVQDAGSAP
                     EEGIDLLPPRSQEHIEEVENKDTAFEETAEVPAANCTDFYCYEKHYQNMVASGTISAA
                     FVDLKVRYDQDTTVRAFCHPLVHVIGRAATKKYPDVSQAYTKGDHFCWSGYYHGVMEG
                     ILYETAPEDLPGVINSICQSIPGRESYSFDYYNCVHGLGHGVMYVTQSELFEALALCD
                     NLQGGWEKESCYGGVFMENVITDFENHFTKYLKPEEPLYPCTAVPEKNKHACYLMQTS
                     YVLKVNGYNFADAFAQCSTIEEQHKDTCYQSVGRDASGSTVSDVARTKAYCLLGTDER
                     QQTNCVIGAVKDFISYFHSDTQAKQFCNAFGDHTQKVCLDTTANYYQSFQKP"
     gene            4425..4568
                     /locus_tag="VFE94_01265"
     CDS             4425..4568
                     /locus_tag="VFE94_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49764.1"
                     /translation="MRKITTYRTPSSFQYKQKANEAKKTQAKRPTRSQNLAKRNTYKG
                     GKD"
     gene            4629..5960
                     /gene="purD"
                     /locus_tag="VFE94_01270"
     CDS             4629..5960
                     /gene="purD"
                     /locus_tag="VFE94_01270"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZX49765.1"
                     /translation="MIFSSGGKVVADTFNTLVVDYGARGHALGLWLRQSPQCDGLFFT
                     GDNDGMAQAGTILSISDPTDIDAIEKAVVDHNVDLVVGGTERALAAGLTDRLKKRLRP
                     VPTFGPCQNAVFHTEASKGKAYKLMAEAGIPCPETWLFGSQAKALAFAEQHKGEPLVV
                     KADGLGEHGNAVFICDSSEEVVAAIKACMEERRFGVAGDHIVIQRKEEGDGQGDEEMS
                     AFFFVDGKNCSDGMGVRDYKRLRTGDTGPMTGGMGSYAPHRRWTPALGKWIADNIVRP
                     LIQALVRRGIAYRGVLYVGIMFTKEGPKVLEFNCRFGDPEAQVLLPLILNDPYEIICA
                     CINGNLARTPIYYKRTMYYVGAVMASGGYPYAGEYKTGYEITGHDKLKASTRVLHAGT
                     RRVGNKVETAGGRVLTVLGWGRTLPKAAQKVNERLAEVNFRNSTFRLDIGS"
     gene            6012..>6288
                     /locus_tag="VFE94_01275"
     CDS             6012..>6288
                     /locus_tag="VFE94_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49766.1"
                     /translation="MDITQLPVIRFCIRYVKTYKIKSALIGGALLVLAGMGVQTLLQQ
                     VSGEPTLERTPRVSLLNVDEYRLENAVVESVGEVESLSQVELKSQVSE"
BASE COUNT         1713 a         1383 c         1717 g         1475 t
ORIGIN      
        1 tatgtatggg tgggggcggt ttccttgtcg ccaaagtaat aggcccgtga tacgctttca
       61 aaaggtaccc gggaaagtat gccaatgcca aaagggaaac ggcccatcaa agccatttgg
      121 ccaaaaaggg gagtcatgcc aagctttgtt gccaggtttt gcagatcggt ttcgtatatt
      181 tcctgcaggc acgccacatc tggctgctcc tctttcagaa aattctctac caggtcgaga
      241 tgtttgtcga gttcgatgtt tatagaagcg agttttatca taggaatgcg tcgcaactac
      301 tatagcataa cttttttctc tttgctatgg ttaggttatg gcaaagcaaa tacgtaccaa
      361 acgcctgctt ctcaggcagt acaaaaaagg agacgaggaa tcttttttgg ccaacatcaa
      421 caatcccaaa gtttcgcgct ggacgagtgg gattccgtat ccctacagca aagaagatct
      481 cgaaacgggg caaactattt tggcgaagaa acaggggaaa acagaaatct cgtttgtgat
      541 cacgcgcgaa aacgaagcca ttggcggagt tagtctgcgg gggattcgca aaggccacaa
      601 agcccgcata gggttttggt tgggcgaagc ctattggggc caaggcttta tggttgaggc
      661 aatttacgca ataacaaagc ttggatttaa agagtttggc ttgcgaagaa tcgaggccaa
      721 agtttttttg cccaataaag gctcaaagcg cgtacttgaa aaagcagggt tcaggctcga
      781 agggctcctc gaaaaagagt acttgaaaaa cggaaagttt attgacgcgt atttgtttgc
      841 aaaggtaaaa taatagatat ggcttctgtg caatttcaaa cagcggtatt tgggggcggg
      901 tgtttttggt gtggcgaggc agtgttccag cgtttgaagg gagtgcaaag tgtaacttcc
      961 ggatacgcgg gtgggagtat ggaaaatccg agctaccagc aggtgtcgag cggccaaacc
     1021 ggacatgcgg aagtagtaaa agtggagtac aatcctgcag ttctttcata cgacaccttg
     1081 ctgtcggtgt ttttcgcgac gcacgatccg accactatga accggcaggg gagtgatata
     1141 gggccgcagt atcgttctgc cattttttat acgagtgagg agcaaaaaat ggaagcagaa
     1201 aagtttatgc aaaagcttgc agacgacaaa gttttcaaag ctcccattgt tacggaacta
     1261 aagcctttgg aggcttttta cgaggcagat gcttcccaca aagattatta caaccgcaac
     1321 aaagaagctc cctattgtca ggtggttatc gatcccaaaa tccaaaaact ccaggagaaa
     1381 tacgcgcatc tcctaaagta gttgttgttt gacgtagaat taaattcgtt gtacaatatc
     1441 gttacaaact tctgggaatg agggaggggc gatgcccact gcagcagaac tgaaagatct
     1501 ttattacgat cggggagagt tcgacaactg ccttgtagcg atccaaaacg aacttgttcg
     1561 ccacgcacaa gacgacccgg aaagaagcga gcttttagct cttgccggct ggtgtcgtta
     1621 tcgaaaaaga gaatacgaaa ctgccaggca actatggcaa gaggcagggc aagtacgttt
     1681 tgcccgagaa ggattggcat acttagccgc ctatatcgac aaagacgatc ttgctcttgg
     1741 cgaactcatc cgggaactag gtgatagcgt aaatgcgcaa aacgctttta ccattcgagc
     1801 gcgcgaccaa gagagtacca tttcccacga gcaagtaatg gaaggggtgc tgcgattcac
     1861 tggcgatacg acagtggaaa tggcaaacct ttaccacaac gctggacgag tattttttca
     1921 taaagctcgc ggcagagaag atttgataat cgcgcttggg ttatacgatg ttgctagggc
     1981 tcgttacggc attgatcgta attggcacca tcgaggagct ctccactatt ggcgttcgcg
     2041 agttttcgag gttttactcg ataaacgggc agcgctcgag gcggctcgag actccttgta
     2101 ctgttggaca cagcaagtta ttcttgatcc tgccacagaa agtcataaac ggaattggga
     2161 aaatgcggtg aatcgcgtgc gtgaactagt cgcagctctt tagggtacaa aagtatcttg
     2221 gctgacggag aatgcgtact gcaaccgccg gctttttatt ttggctgatt tttggtagga
     2281 tagagtgtat gaaatttttg ctggcgacaa caaacgaggg caaaatccgg gagtataggc
     2341 acttgctggg taaagcgggt gttgagtttg ttacgcttgc ggaagctggc attacagaca
     2401 aacccgaaga aacaggcgcc acgtgcgcgg aaaacgccat actcaaggca aaattttatt
     2461 ttgaaaagtc gggcggcatg cccacgctag ccgacgacgg gggattggaa atcgacaccc
     2521 tgggcgggga gcctggcgtg cattccaggc gctggccggg ccacgaagcc acagacggag
     2581 agcttgtgca atacacgtta gagcgcatgg aaggtattgc cctagaaaaa cgcaccgcca
     2641 gacttacggc tgtgcttgcc gtaaccgcag acggcaagaa tgtgcagacg ttttcaggca
     2701 cgctcgaagg gaaaattgtg gaaaagccgt acaacccgat tgtcgcgggg tacccgtttc
     2761 gctcgttatt tcaggaaatg caaacaggca gagttcttgc ggaaattccc gtagagcagc
     2821 ttcccaacgc gcaccgggaa caggcaatcg caaaagccct gccccttctt caacaacttg
     2881 ccaaaaattg gtagaataaa ggaaacttat ggacaagctt acgccagaac aatatcgcgt
     2941 aatgcgagaa aagggcacgg aagccgcctt ttcgggaaag tattgggatt cgcatgagaa
     3001 aggcatgtac aagtgcgcct cgtgcggaaa agagcttttc tcttctgaca ccaaattcga
     3061 ctcgggtacg ggctggccaa gctttaccga gcctgccaac ttggcgaaca ttgaactcaa
     3121 agaagataac agccacggca tgaaaagaac cgaagtgctg tgcaagaatt gtggcgcgca
     3181 cctgggccac gtatttaacg acggccctgc agggcaggga ggcaaacgct attgtattaa
     3241 ctctgtgtgt ctggaacttg aaaaagaatg aagaaaacgt atctcttttt gattatcgga
     3301 gctagtatgc tggcgggggc cggaattttt ttagctcaag gattttttga caataaggaa
     3361 gcggatgcgt tacttgttca ggacgcgggt tccgcgccag aggaggggat tgatcttttg
     3421 cctccgcgct cgcaagaaca tattgaagaa gtggaaaaca aggatacggc ttttgaggaa
     3481 acagctgagg tgccggcagc taactgcacg gacttttact gctatgaaaa gcattaccaa
     3541 aatatggttg cctcgggcac gatttcggca gcgtttgttg acctgaaagt acggtacgat
     3601 caagatacca ccgtgcgcgc gttttgccac ccgttagtcc atgttatagg ccgggctgcc
     3661 accaagaaat accccgatgt atcacaagcg tatacaaaag gggatcactt ctgctggtct
     3721 ggatactatc acggcgtaat ggaaggcata ttgtacgaaa ccgcgcccga ggatttgcca
     3781 ggagtgatta actctatttg ccagagcatt ccggggagag aaagctattc ttttgactac
     3841 tacaactgcg tgcatgggtt agggcacggc gtaatgtatg ttacgcaaag cgagttgttt
     3901 gaggcattgg cgctgtgcga caacctccag ggcggatggg aaaaagagtc gtgctacggg
     3961 ggcgtgttta tggaaaacgt cataaccgat tttgagaatc attttaccaa atacttaaaa
     4021 ccagaagaac ctttataccc atgtaccgcg gtgcccgaaa aaaacaaaca cgcgtgctac
     4081 ttaatgcaaa cttcatatgt gctcaaagta aatggataca actttgccga tgcttttgcg
     4141 cagtgttcca ccatcgagga gcagcataag gatacctgct accaaagcgt ggggcgcgac
     4201 gcttcgggtt ccacggtaag cgacgttgcg cgcacgaaag catactgctt gctgggtaca
     4261 gatgaaaggc agcaaaccaa ttgcgtaatc ggcgccgtaa aagatttcat ttcgtatttc
     4321 cactccgaca cgcaggcaaa gcaattttgc aacgctttcg gcgatcatac gcaaaaggtc
     4381 tgtttggata ccacagcaaa ttattaccag agttttcaaa agccatgaga aaaatcacca
     4441 cataccgcac gccctcgagt tttcaataca aacaaaaagc aaacgaggcc aaaaaaaccc
     4501 aggcaaagcg ccccacccga tcgcaaaact tagccaagcg caacacatac aagggcggca
     4561 aggactagtt tgccaacccg ctaaaatttg ctatcctacc tatagcacat gcgctttttg
     4621 cgtatgtttt gatttttagc tccggaggga aagtcgtggc agatacattc aatacgttag
     4681 ttgttgacta tggcgcacga ggtcatgccc ttggcctttg gctaaggcaa agtcctcaat
     4741 gcgacggttt gttctttacc ggggataatg atggaatggc gcaagccggt accatcttat
     4801 cgattagcga cccgaccgat atcgacgcta tcgaaaaggc ggtggttgac cataacgttg
     4861 atcttgtggt tggcggtaca gaacgggcgc ttgcagccgg tttaacagat cggcttaaga
     4921 aaaggctacg gccagtccca acctttgggc cgtgccagaa tgctgtgttt cataccgaag
     4981 ccagtaaagg caaggcctat aagctgatgg ctgaagcagg tataccgtgt ccggaaactt
     5041 ggctttttgg tagccaggcg aaagctcttg cgttcgccga gcagcataag ggagagccct
     5101 tggttgtaaa agccgatggg ttaggggagc acggaaatgc tgtgttcatt tgcgactcct
     5161 ccgaagaagt agttgctgct attaaggctt gcatggagga gcgtcgtttc ggcgtagcag
     5221 gagaccacat tgttattcaa cgcaaagaag agggcgacgg ccaaggagat gaggaaatga
     5281 gtgccttttt ctttgtcgac ggcaaaaact gttccgacgg catgggagtt agggactaca
     5341 aacggttgag gactggtgat acaggcccta tgaccggagg catgggcagt tatgctcctc
     5401 atcgtcgctg gactcccgcc ttgggtaagt ggattgccga taacatcgtg cggccattga
     5461 ttcaagcgct ggtgcggcgc ggtattgcgt atcggggcgt tctgtatgtg gggattatgt
     5521 ttaccaagga aggtcccaag gttcttgagt tcaattgccg ttttggtgat cctgaggcac
     5581 aggttttgct cccgcttatc ttgaacgatc catacgagat aatatgcgca tgcatcaacg
     5641 gcaacctggc gcgcacgccc atatactaca agagaaccat gtattacgtt ggggcagtaa
     5701 tggcctcggg tgggtaccct tacgcagggg agtacaagac cgggtacgaa ataacgggcc
     5761 acgataaatt aaaagcaagt acgcgagtgc tgcacgcagg aactcgtcgc gttggcaata
     5821 aggtggagac tgccggagga cgtgtactta cggttctagg ctggggcaga acgctaccca
     5881 aggcagctca aaaagtgaac gaacgactgg ctgaagtgaa cttccgtaac tctacgtttc
     5941 ggttggacat tggaagctag agaggaggtc tttgacctcc ttgttttttt agcgtataat
     6001 atggctggtt tatggacatt actcaattac ctgtaattcg attttgcatt cgatacgtaa
     6061 agacctataa aataaaatct gccttaattg ggggagcttt gctggttttg gcaggcatgg
     6121 gggtgcaaac attgttgcaa caggtaagcg gcgagcccac cctcgagcgc acgccacggg
     6181 taagcctttt gaatgtagac gaataccgat tggaaaacgc ggttgtagag tcggtaggcg
     6241 aggtggagtc gctttcgcag gtggagctca aaagccaggt gtcagaac
//
LOCUS       DASRUP010000030         4456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_858671, whole genome shotgun sequence.
ACCESSION   DASRUP010000030 DASRUP010000000
VERSION     DASRUP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858671"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="tsaD"
                     /locus_tag="VFE94_01280"
     CDS             complement(<1..722)
                     /gene="tsaD"
                     /locus_tag="VFE94_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZX49767.1"
                     /translation="MTILGIETSCDDTGVAVLETTEGKNPQFAIRSNVVASQIDVHQE
                     YGGVFPSLAKREHIKNLPITLEKALKGAKVEKTDAIAVTYGPGLSPCLWTGINFAKDL
                     AKEWNVPLVPVDHMEGHLLVAFLGDENFQFSNPNFQKLFPAVALIVSGGHTQLVLVKD
                     IGKYQMIGETRDDAAGECFDKTARILGLPYPGGPAVAAAAALAGKPAIGIKLPRPMMH
                     TKDYDFSFSGLKTAVLYDYKARP"
     gene            797..871
                     /locus_tag="VFE94_01285"
     tRNA            797..871
                     /locus_tag="VFE94_01285"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:832..834,aa:Glu,seq:ttc)
     gene            878..2311
                     /locus_tag="VFE94_01290"
     CDS             878..2311
                     /locus_tag="VFE94_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZX49768.1"
                     /translation="MEQEAQNQKRSIPRMFFLAGISLVLLAGTFLVWSVLFQSFGSKA
                     MESSIASVKESILPLNLATKPNPTYLNRWAEARKALVEAQENFLEVNFSSNKVIVYKA
                     GVAISEAPIYAKGDPQGWGGTPAGLYSVLDMHKVAYSGIAEVNMPYAIHFYGKYYVHG
                     ETYYTSGIKTGDDVSGGCVQLLESDAAKVFAAAEPAMPVLVIDKENDSYSYEKISSGE
                     PPLVSASSYLVTDLDSGFVFAEKNSQRVSSIASLTKLMTATVVAENIDLRKSVEVTPA
                     MLEAYGETDALLLGERYRVVELFYPLLISSSNDAAEATAGFLGRERTIRMMNEKAEAL
                     LMQDTKFVDPSGLDSGNVSTAQDLFHLARYVVNNRPPIFEITKGEVVRTYGTISFAVQ
                     GLWNKNLFVEDTSFAGGKTGYTKAARHTGIFLFRFRAEQGALRTIAFVVLDSRNDKLD
                     VQRLYGWLERNYNLKPDYEDTQLSNAS"
     gene            2315..3871
                     /locus_tag="VFE94_01295"
     CDS             2315..3871
                     /locus_tag="VFE94_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX49769.1"
                     /translation="MQAAPHLVAAKAADLEDPRDRFAYRALEMFPGIFAWGTLVFAVV
                     GSWLFPAVVAFFVLTFVLYWLLRVMYFSIHLRAGYKRTREHEKTDWLQKLNEVPKARY
                     AIPVSHWQDVYHLVVVPTYKESLEILRGTFLALKNSDYPKDKMIVVLGIEERAGQQGA
                     DRARTIEQEFGNEFFRFLVTVHPQDLPGEIAAKGSNETWAVRQAKKEIIDPLGIPYEH
                     IVMSSLDADTVVFPKYFSCLTYHYLTSKNPLRNSYQPIPLFINNIWQASIPSRLFAFA
                     TTFWFLMNQERPEKLSTFSSHSMPFKTLVEVGYRQVNYAVDDSRIFWQCFLHYDGNYS
                     VQALYYPVAMDANTALTLWGTIRNIYKQQQRWAYGVADIPYFLFGFLKNKKIPLRKKI
                     SLGLDRVESYWSWATASPLIFLLSWLPVLLGGDVFNQTLLSYNLPRVTSWILTVMMAG
                     LVGSAYLSLVFLPPRPPNYGKWRLAWLAVQWVFLPPIMLFFAIPALHAQTRLMLGRYM
                     GFWSTPKFRK"
     gene            complement(3872..>4456)
                     /locus_tag="VFE94_01300"
     CDS             complement(3872..>4456)
                     /locus_tag="VFE94_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZX49770.1"
                     /translation="VKTVKEEYEKLSKEVFPKLRIEMLHGKMPGKEKEQVMRQFKFGK
                     IDLLVSTSVVEVGVDIPNATVMMIEGADRFGLAQLHQFRGRVGRAEHQSYCFLFAESN
                     SFIARKRLKALEESNSGFELAERDLQIRGPGDFAGTKQWGLPDFAMKNLTNLELVEKA
                     RESAKAILEKDLTLKNYPLLQEKVADFRQKLHLE"
BASE COUNT         1032 a         1054 c         1110 g         1260 t
ORIGIN      
        1 ggccgtgcct tgtagtcgta gagcaccgcg gttttcaggc ccgaaaaact aaagtcgtag
       61 tctttggtat gcatcatggg ccgcggcagt ttgataccaa tggctggctt gcccgccaaa
      121 gccgccgcag cggcgacggc ggggccgccg ggatacggca agccaagaat gcgggctgtt
      181 ttgtcaaaac actcgccggc tgcgtcgtcg cgcgtttcgc cgatcatttg gtatttgccg
      241 atatctttta ccagcaccaa ctgcgtgtgc cctcccgaaa ctattaaagc aacggccggg
      301 aaaagttttt gaaaattggg atttgaaaat tgaaaattct cgtcgccaag aaaagcgacg
      361 agcaggtgtc cctccatatg atctacgggc accaatggta cgttccattc ttttgccaaa
      421 tctttggcga aatttatgcc tgtccagagg cagggcgaca agcccgggcc gtatgttacg
      481 gcaattgcgt ctgttttttc tacttttgct cccttgagcg ctttctcgag cgttatgggt
      541 aaatttttta tatgctcccg ttttgcaaga ctggggaata caccaccgta ttcctggtgt
      601 acgtcaattt gcgaagctac tacgtttgag cgtatagcaa attgcgggtt tttgccctct
      661 gtggtttcca aaaccgcaac tccggtgtcg tcgcacgagg tttcaatgcc taaaatcgtc
      721 atagcccttt tctacctctt ttctttacaa atttcaattt ttaagctaaa gtatggaaga
      781 caagcaggtg gtttttggtc ccatcgtcta gtcggtctag gacgccgggt tttcatcccg
      841 gaaatcaggg gttcgaatcc ccttgggacc gcctcgtatg gagcaagagg cgcaaaacca
      901 gaaaagaagc attcctcgca tgttctttct tgcggggatt tctttggtgc ttttagcagg
      961 cacttttttg gtgtggagcg ttttgttcca atcttttggg tccaaagcta tggagtcttc
     1021 gatagcttcg gtaaaagaga gcattctccc gctcaatctc gcaacaaaac caaacccaac
     1081 gtatctgaac cgttgggcag aagcaagaaa agcgttggtt gaggcccagg aaaattttct
     1141 tgaggtgaac ttttccagca ataaggttat cgtgtataaa gctggagtgg caatcagcga
     1201 agctcccatt tacgcaaagg gagatcccca ggggtggggc ggcaccccgg ccggcctcta
     1261 tagcgtgtta gatatgcata aagttgcgta ttcaggcatt gcagaggtaa atatgcctta
     1321 cgccattcat ttttacggaa aatattatgt gcacggcgaa acctattata cgtcgggtat
     1381 aaagaccggc gacgacgttt cgggcggttg cgtgcagttg ctcgagtccg atgcggcaaa
     1441 agtatttgcg gcagcggaac ccgcaatgcc ggttttggtt atcgacaagg aaaacgatag
     1501 ttactcttac gagaaaattt cgtctggtga accgcccttg gtttcagcct cgagctacct
     1561 agtgacagat ttggactccg ggtttgtgtt tgcagaaaaa aactcgcagc gggtttcgtc
     1621 tatcgcttct cttaccaaac tcatgaccgc tacggtggtg gcagaaaata tcgaccttcg
     1681 aaagtcggta gaagtcacgc ctgcaatgct tgaggcgtat ggcgaaaccg acgcgttgct
     1741 ccttggagag cgataccggg tggtggagct gttttatcct ttgttaattt cttcttccaa
     1801 cgatgcagca gaagctacgg ctggattctt gggccgcgaa agaactattc gcatgatgaa
     1861 tgaaaaagcc gaggcgctct taatgcaaga tacaaaattt gtcgaccctt cgggattgga
     1921 ctctggcaac gtttccacag cgcaagactt gtttcacctc gcaagatacg tggtaaacaa
     1981 ccgtcccccc atttttgaaa ttaccaaagg agaagttgtg cgaacgtacg gaaccatttc
     2041 gtttgcggtg caggggttat ggaacaaaaa tttgtttgtt gaggatacga gctttgctgg
     2101 gggcaagaca ggatatacga aagcggcgag gcatacgggc attttccttt ttcgttttcg
     2161 agccgagcaa ggagcactgc gtaccattgc ctttgtagtg ttggattctc gaaacgacaa
     2221 gctcgacgtg caaaggctgt acggttggct cgagagaaac tacaatctca agcccgacta
     2281 cgaagatacc caactttcta acgcttctta aattatgcaa gctgcccctc atcttgtagc
     2341 tgccaaggca gccgatcttg aagatccgcg cgaccgattt gcctatcgcg ctttggaaat
     2401 gtttcccgga atttttgctt ggggtacgct tgtgttcgcc gtggttggtt cctggctgtt
     2461 tcccgcggtc gtcgcttttt ttgttttaac atttgtactc tattggcttt tgcgcgtgat
     2521 gtatttttcg attcatttgc gcgcaggcta taaaagaaca cgggaacacg agaaaaccga
     2581 ctggctgcaa aagctcaatg aggtgccgaa agcaaggtat gccattccgg tttcccattg
     2641 gcaggatgtt taccatttag tggtggttcc cacgtataaa gagtcgctcg aaattctccg
     2701 ggggactttt ttggcgctca aaaattcaga ttatccgaaa gataagatga ttgtggtatt
     2761 gggtatcgaa gaaagggcgg ggcagcaagg ggccgaccgc gcacggacta tagagcaaga
     2821 attcggcaac gagtttttcc gttttttggt tactgtgcat ccccaagact tgccgggcga
     2881 aattgcggcc aaagggtcga acgaaacgtg ggcagtaagg caggcaaaaa aagaaataat
     2941 cgaccctttg ggtattccgt atgagcatat tgttatgagt tccttggatg cagatactgt
     3001 agtgttcccc aaatacttca gttgtctcac ctatcactat cttacttcca aaaacccttt
     3061 acgaaattcg tatcagccca ttcctttgtt catcaacaat atttggcagg cctctattcc
     3121 atccaggctt tttgcttttg ccaccacctt ctggttttta atgaaccagg agcgccccga
     3181 aaagctaagc acgttttctt cgcattccat gcctttcaaa acgcttgtcg aagtcggata
     3241 caggcaggtg aactatgcgg tggacgattc ccgcattttc tggcagtgct ttttgcacta
     3301 cgatgggaat tactcggtgc aggcgttgta ctaccctgtt gccatggacg ccaataccgc
     3361 tcttacgctt tggggaacta tccgtaacat ctacaaacaa cagcagcgtt gggcttatgg
     3421 cgtggcagac attccgtatt ttttattcgg gtttttgaaa aacaaaaaaa ttcctttgcg
     3481 caaaaagatt tccttggggc ttgaccgcgt ggaatcgtac tggtcgtggg ccacagcttc
     3541 tccccttatt tttcttttga gctggttgcc ggtgcttttg ggaggagacg tattcaacca
     3601 aacattgctt tcgtataatc ttccgcgggt tacttcgtgg attcttactg ttatgatggc
     3661 agggctcgta gggtctgcgt atttgagctt ggtatttttg ccgccccggc ctcccaatta
     3721 tgggaaatgg cgattggcct ggctagcagt acagtgggta tttctgccgc ctattatgtt
     3781 gttttttgct atacctgcgc ttcacgccca aacccgttta atgttgggcc gctacatggg
     3841 tttttggtcc acccccaagt ttcgaaagta gctattccaa atggagtttt tggcggaaat
     3901 cggctacctt ttcttgcaag agagggtagt tttttaatgt aaggtctttt tccaaaattg
     3961 ctttggctga ttcgcgggct ttttccacga gctccaggtt ggtaaggttt ttcatggcaa
     4021 agtcgggcaa gccccattgc ttggtgcccg caaagtcgcc gggcccccgg atttgcaaat
     4081 cgcgctctgc caactcaaac ccgctgttgg attcttccaa ggcctttaat cgtttgcgcg
     4141 caataaacga gtttgattct gcaaacaaaa agcaatacga ctggtgttcg gccctcccaa
     4201 cgcggccccg aaactggtgc agctgggcca agccaaatcg gtctgcccct tctatcatca
     4261 ttaccgtggc attgggaata tccacgccaa cctcaacaac cgaagtggaa accagcaaat
     4321 caattttgcc gaatttaaat tgccgcatta cctgctcttt ttctttgcct ggcattttgc
     4381 cgtgcagcat ttctatgcgc agcttgggaa atacctcttt ggaaagcttt tcgtattctt
     4441 cttttaccgt ttttac
//
LOCUS       DASRUP010000031         6776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1625882, whole genome shotgun sequence.
ACCESSION   DASRUP010000031 DASRUP010000000
VERSION     DASRUP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625882"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFE94_01305"
     CDS             complement(<1..1452)
                     /locus_tag="VFE94_01305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZX49771.1"
                     /translation="MAFFSMRHDALTFDELAHIPAGYSYLTQQDYRINPEHPPLAKDI
                     AAFPLIFLDLNFPANSQNWLQDSGAPPWWVQFDLGNEFLYKSGNNPLSIIFWSRLPMI
                     LLTLALGAVLFFWTRKLLGNGAALAALTLFAFSPSFLAHGRLVTTDVAAALGILVAAY
                     FWIEFLKQPTKTKVGKAGFALALAFLFKFGAVLLLPFFAVITVIYALLHKESSGNMRT
                     LGKYVGLSVLVGIVALFLIVPVYQLHIVNYPAERQLRDTVSDLQPGGVTAYEQAIIWM
                     ADKEPFRAFAQFGRGVLMATQRGQFGNTVFFQGEIKVGGFWQYFPLIYLYKVPLAFHV
                     LALFGILGILWSFQKQWYRGIKEMLLTWWIKKHFTFFAFVVFIAGYFLVAMTGNLNIG
                     VRHLLPVFPLLYILVVWGLKELLSHIENNQFKRIAVSFVAGLFVWYIGSSLMAFPNYI
                     PYYNALAGGTENGYKIAVDSNYDWGQDFYRLLDF"
     gene            complement(1518..2432)
                     /locus_tag="VFE94_01310"
     CDS             complement(1518..2432)
                     /locus_tag="VFE94_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZX49772.1"
                     /translation="MHKIIQGPKVTWIDIQNPTQEDIDFLKENNGFHPLVLDELIPPA
                     WRTKVEAFPSYLFFISYFAAWSKEHKHTRPREIDVIVGKNILVTCHYNSIVPLKALFD
                     ACNLYEDKRREYMSQGGGLLLFHAMDAMRQNTLIKIDRISKKLHHIEEQIFLGHERQM
                     LQEISYAKADIINFWRIVHPQGEILESLQKQGKDFFGPDLDPYFSRLLGDWSQSRNTL
                     ETFKESIEALEATNNSLLAEKTNEIVKVLTIVSASVLPLTLLAGLFGMNTEHTPLMGM
                     PGDFWILVGVMVLGVFLLVAYFKHKKWL"
     gene            2502..2930
                     /locus_tag="VFE94_01315"
     CDS             2502..2930
                     /locus_tag="VFE94_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49773.1"
                     /translation="MKAVAVVLAIFFLSVACTATSHVAPAPVPTLAAPSLDPAMIFTQ
                     VPVFVEFRYDVNDPYSAMTAHIMLDNIFKERVVVRLEAKESGSFKMDTFNVVPDKHND
                     VFTWKALQIEPGERITIVGKGRRTGLETPKFAFDIETIRQ"
     gene            3030..3662
                     /locus_tag="VFE94_01320"
     CDS             3030..3662
                     /locus_tag="VFE94_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49774.1"
                     /translation="MVLSERQKDILQKAVFEYIDRAEPVSSGWLEEQYELPFSPATIR
                     AEMMQLTEQGYLEQPHTSSGRVPTDKGYRFFVDELRPAELEDEQPFGFSALALAYYHR
                     TLWKENWERVLREPEFSRAELLGNFTKFISDIEQNSSRIRRVRQLEVYIGKENPFSSV
                     GDFSMILSECDFAQGQHGFVAIVGPKRMPYQKNIGFIHSLIQTWKKKLKR"
     gene            3638..4084
                     /locus_tag="VFE94_01325"
     CDS             3638..4084
                     /locus_tag="VFE94_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZX49775.1"
                     /translation="MEEKIEKITQERDEYLAGWKRAKADFINYKKEEAQRMQVMAGYA
                     YQQLLLMVLPILDNVDRAVKQIPEEEKDNQIYKGFLQIASQWREFLKSQGIEEVETVG
                     KPFDPEMHEAVGEVGGEEPGMVAEEVEKGYIINGKLLRPAKVKITK"
     gene            4091..5908
                     /gene="dnaK"
                     /locus_tag="VFE94_01330"
     CDS             4091..5908
                     /gene="dnaK"
                     /locus_tag="VFE94_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZX49776.1"
                     /translation="MAKILGIDLGTTFSAMAVVEGGEPRIIENREGARTTPSVVALAK
                     NGETLVGVQAKRQQVTNPQNTIFVVKRLIGRRFSDAEVQKDKKLLPYEIREAQDGGVE
                     VKMSDKWLKPAEISAMVLRKLKQDAEDKLGEKVTEAIITVPAYFDDSQRKATKVAGEI
                     AGFDVKRVINEPTAAALAYGLSKNKEQKIIVYDFGGGTFDISILDVSEDTIEVKATGG
                     DTHLGGEDFDQKVVNWLIEQFQKDEGIDLSKDTLALQRLKEGSERAKVELSSAMETEI
                     NLPFITSDSSGPKHLYYKLTRAQLEGMVREYIDKSVELVKQTLQEAKLSPGDIQEIVL
                     VGGQTRMPAMQEAVKALFGKEPNKSINPDEVVAIGAAIQAGIMQGDVKDVLLLDVTPL
                     SLGIETLGGVNTVLISKNTTIPTSKSQVFSTAADNQTSVEVHVLQGERPLAKDSKSLG
                     RFILDGIPPSPRGMPQVEVSFDIDANGILQVSAKDKASGKTQSIRIEGSTGISEQEVE
                     RMKKEAELHATEDKKKQELIEARNAADAIIYTSEKTLADVRDKVKEEDVKETGEKLDA
                     LKKVKESDNIEEIKKATEELSQAIQKVGAAMYQNPNPQA"
     gene            5905..>6776
                     /locus_tag="VFE94_01335"
     CDS             5905..>6776
                     /locus_tag="VFE94_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZX49777.1"
                     /translation="MKDYYKVLGVSRDASFEDIKRAYRKLAHKYHPDIKGGDEEKFKE
                     LNEAYQILSDSDKRSQYDKFGRVFDGQAGADPGFGGFRWAWGTPGQQADFEDSAAGFG
                     FDFEDIGDVFEEFFGGQTRDTRKQEVKKGRDVEVELQIPLEATLRISKEKIFLSKFAV
                     CQRCQGVGAEPNTKVTECFACRGQGEVQQIKRTVFGSFTRVGTCPECAGEGLKPEKPC
                     NVCKGEGRVKSEEQLEVTIPAGVDTNQILRVEGKGDAGRRKGKAGDLYIRIVVKKHPL
                     FERRGDDVVLKKDIA"
BASE COUNT         2006 a         1447 c         1809 g         1514 t
ORIGIN      
        1 aaaatcgagt aggcggtaaa aatcctggcc ccagtcgtag ttggaatcca cggcaatctt
       61 gtaaccgttt tctgtgccgc cggccaaagc attgtagtaa ggaatataat tgggaaacgc
      121 cataagagaa gatcctatat accaaacaaa aagccctgct acgaaactta ccgctattcg
      181 tttgaactgg ttgttctcaa tgtgagaaag cagttcttta agcccccaaa caaccaatat
      241 gtaaagaaga ggaaaaacag gcagcaaatg ccgcacgcca atgtttaagt ttcccgtcat
      301 tgcaaccagg aagtaccccg cgataaatac cacaaacgcg aaaaaggtaa aatgtttttt
      361 tatccaccac gtgagcagca tttcttttat gccgcgatac cactgttttt ggaaactcca
      421 cagaattccc aatatgccga aaagcgcaag tacgtgaaac gcaagaggaa ctttatataa
      481 gtaaataaga ggaaagtatt gccaaaaacc gcctacctta atctcgcctt ggaaaaacac
      541 ggtgttgcca aactgcccgc gttgggtggc cattaaaaca ccgcggccaa actgggcaaa
      601 cgcgcgaaac ggttctttgt ctgccatcca aattattgct tgctcgtatg ccgttactcc
      661 gccaggctgc aagtcggaaa ccgtgtcgcg gagctggcgt tctgcagggt agtttacaat
      721 atgcagctgg taaaccggca caataaggaa aagcgccacg atacccacca aaacagaaag
      781 ccctacatat tttcccagag tgcgcatatt gccagaagac tctttgtgaa gcaaggcata
      841 tataacagta atgacagcaa agaaggggag caacagaaca gcgccgaatt taaacaagaa
      901 tgcgagcgca agcgcaaatc cagcttttcc tacttttgtt ttggttggct gctttagaaa
      961 ttcaatccaa aaataagcgg caaccagtat gcctaaagcc gcggcaacgt cggtggttac
     1021 cagccttcca tgcgccagaa agctgggtga aaacgcgaac aatgtaagcg ccgcaagcgc
     1081 ggcgccgttg cctagcaact ttcttgtcca gaaaaacaaa acagctccca aagccaaagt
     1141 aagcaaaatc atgggaagcc gggaccaaaa tattatggaa aggggattat tacccgattt
     1201 atataaaaat tcgtttccca aatcaaactg cacccaccaa ggcggagcgc cggaatcctg
     1261 tagccagttt tggctatttg cgggaaagtt caaatccaaa aagatcaggg gaaaggcggc
     1321 aatatctttt gcaagcggcg gatgttccgg gtttatccga tagtcttgct gggtaaggta
     1381 agagtagcct gccggaatat gggcgagttc gtcgaacgtt aaagcgtcgt ggcgcatact
     1441 aaagaacgcc ataaaaaaca tagctccgag caaaaatacg gcaaagagat aagtggcagc
     1501 attcgaaaga ggaagtttta cagccatttt ttatgcttga agtatgctac caggagaaac
     1561 acgcctaaca ccataacgcc aactaaaatc caaaagtctc cgggcatgcc cattaacgga
     1621 gtgtgttcgg tgttcatgcc aaacaggcct gccaaaagcg taagcggaag aacgcttgcc
     1681 gacacaatgg ttaagacttt tacgatttca tttgtttttt cggcaagcaa ggagttgttt
     1741 gttgcttcta aggcctcaat agattctttg aaagtttcga gcgtgtttct cgactggctc
     1801 cagtcgccca gcaagcggct aaagtagggg tcgaggtctg gcccaaaaaa gtcttttcct
     1861 tgtttttgta gagactctaa aatttccccc tggggatgca caatgcgcca aaagttaata
     1921 atgtctgctt tggcgtacga gatttcctgg agcatttggc gctcgtggcc caagaaaatt
     1981 tgctcttcaa tatggtgcag ttttttgcta atccggtcta tttttatcag cgtgttttgg
     2041 cgcatggcat ccatggcgtg aaacagcagc aaaccccctc cctggctcat gtattctcgg
     2101 cgcttgtctt cgtataaatt gcaggcgtca aacagggctt ttaagggcac aatggagttg
     2161 tagtggcagg ttaccaaaat gtttttgcct acaataacat cgatttcgcg tggccgggtg
     2221 tgcttgtgtt ctttgctcca agcggcaaag taggaaataa aaaagaggta gcttgggaac
     2281 gcttccactt tggtgcgcca cgcgggagga ataagttcgt ccaacaccag cggatgaaac
     2341 ccgttgtttt ctttcagaaa atcaatgtct tcctgtgtgg ggttttgaat gtctatccac
     2401 gtaacctttg gcccctgaat aatcttatgc attgatatat tgtaccagat atgctatcat
     2461 cggcaatatt cgcacatcat atatataaag gaaggtgaag aatgaaagcg gtagcggtgg
     2521 ttttagcgat ttttttcttg tcggtggctt gtacggcgac aagtcatgta gcccctgcgc
     2581 cggtaccgac tctggcggct ccatctttgg accccgcgat gatttttacc caggtccctg
     2641 tttttgtgga atttcggtat gatgtgaatg atccgtactc ggcgatgacg gctcatatta
     2701 tgctggataa tatttttaag gaaagggttg tggtcaggct cgaagcgaaa gagagtgggt
     2761 cgttcaaaat ggacactttt aacgtggtgc cagataaaca caatgacgtt tttacctgga
     2821 aagctctaca aatagagccg ggagagcgga tcaccatagt tggaaaggga agaaggactg
     2881 gtttggaaac tcctaagttt gcttttgaca tcgaaacgat caggcaataa acggcatacc
     2941 cgaacctccc taagcggtca atcttaaaga gtgtctttgg cagtcgaggt tcttgactgc
     3001 taattttttt acggttataa taggggaata tggtactttc tgaacgacag aaagacattc
     3061 tccaaaaggc ggtttttgag tatatcgaca gggcggaacc cgtaagttca ggctggctgg
     3121 aagagcagta tgaattgccc ttttccccgg ccacaattcg cgcggaaatg atgcagctta
     3181 cggaacaagg gtatttagag cagccgcaca cgtcttccgg gcgcgtgccc acggacaaag
     3241 gctaccgttt ttttgtggat gagctgcggc ctgccgagct ggaagacgaa cagccttttg
     3301 gattttccgc gctggcgctg gcgtattacc accgcacgct ttggaaagaa aactgggagc
     3361 gggtgctgcg ggaacccgaa ttttcgcggg ccgagctgct gggtaatttt accaagttta
     3421 tttcagacat tgagcaaaac agcagccgca tacgcagggt acgccagctt gaggtatata
     3481 taggaaaaga aaatcctttt tcaagcgtgg gggattttag catgatttta agcgagtgcg
     3541 attttgcgca agggcagcat gggtttgtgg ccattgtggg gccaaagcga atgccctacc
     3601 aaaaaaatat cggatttatc cattctttaa tacaaacatg gaagaaaaaa ttgaaaagat
     3661 aacacaagaa cgcgacgaat acctggcagg ctggaagcgc gccaaagcgg atttcataaa
     3721 ctacaagaaa gaagaagcgc aaagaatgca ggtaatggca ggatacgcct accaacaact
     3781 gcttttgatg gttttgccga ttttagataa tgtagatagg gcggtaaaac agattcccga
     3841 ggaagagaaa gataaccaga tatacaaagg gtttttacag attgccagcc agtggcggga
     3901 attcctgaaa agtcagggaa tagaagaggt agaaaccgta ggaaagccct ttgatccgga
     3961 aatgcacgag gcggttgggg aagttggggg agaagaacct ggcatggtgg cagaagaagt
     4021 ggaaaagggg tatataataa atggcaagct tttgcggccg gcaaaggtga aaatcacaaa
     4081 ataaaacatt atggcaaaaa ttttaggaat tgatttgggc acaacctttt ccgccatggc
     4141 agtggtggaa gggggagagc cccgcattat cgaaaaccga gagggcgcca gaaccacgcc
     4201 ttcggttgtt gcgttggcaa aaaacggcga gactttggtt ggcgtacagg caaagcgcca
     4261 gcaggtaacc aatccgcaga acaccatttt tgtggtaaag cggttaatcg ggcgccgatt
     4321 ctctgatgcc gaagtgcaaa aagacaagaa gctgttgccc tatgaaatcc gcgaggcgca
     4381 agacggcggc gtggaggtaa aaatgtccga caaatggtta aaacccgctg aaatttcggc
     4441 catggtgctg cgcaaactaa agcaagacgc cgaagacaag ctgggagaga aagttactga
     4501 agccattatt acggttcccg cgtatttcga cgacagccag agaaaagcaa ccaaagtggc
     4561 aggcgaaatc gcgggatttg atgtaaagcg ggttatcaac gagcccacgg ctgcagcttt
     4621 agcatacggt ttgagcaaaa ataaagagca gaaaatcatt gtgtacgact ttggaggcgg
     4681 cacgttcgac atctccattc tcgacgtgtc ggaagacacg attgaggtaa aagcaaccgg
     4741 aggcgacacg cacctgggag gcgaagactt tgaccagaaa gtggtaaact ggcttattga
     4801 gcagttccaa aaagacgaag gcattgattt gtcgaaagac acgctggctt tgcagcgctt
     4861 aaaagagggc tcagagcgcg ccaaggtaga gctttcttcg gcaatggaaa cggagataaa
     4921 cctgcctttt attacgtcgg actcttcggg gcccaagcat ttgtactaca agcttacacg
     4981 agcacagctg gaaggaatgg tgagagagta catcgacaag tctgttgagt tagtaaagca
     5041 aacgttacaa gaagcgaaac tttcacccgg ggatatccag gaaatagttc ttgtgggggg
     5101 ccagacgaga atgcctgcca tgcaagaagc ggtgaaagcg ctgtttggca aagaaccgaa
     5161 caagtccatt aatccagacg aggtggtggc aattggagct gccatacaag ctggcattat
     5221 gcagggcgat gtgaaagacg tgttgttgct cgacgttacg cctttgtctt tgggcattga
     5281 aaccctgggc ggggtaaaca ccgtattaat ttcaaagaat acaaccattc ccacgagcaa
     5341 aagccaggtg ttttcgactg cagccgacaa ccaaacttcc gtagaggtgc acgtgctgca
     5401 aggcgagcgc ccccttgcca aagactcaaa atcgctcggc agatttatct tagacggaat
     5461 tccgccctca ccccgcggca tgccgcaggt ggaagtaagc tttgacatag acgccaacgg
     5521 aattttgcag gttagcgcga aagacaaggc ttcgggcaaa acgcagtcta tccgcattga
     5581 gggctccaca ggcatttcgg aacaagaagt ggaacgcatg aaaaaggagg cggaactgca
     5641 cgcgacagaa gacaagaaaa agcaggagct tatcgaggca agaaatgccg cagacgccat
     5701 tatctataca tccgaaaaaa cgcttgcaga cgtcagggat aaggtaaaag aggaagatgt
     5761 taaagagact ggggaaaagt tggatgcttt gaaaaaagtg aaggaaagtg ataatataga
     5821 agaaattaag aaggctaccg aagagctctc gcaggcgatt cagaaggtgg gggctgcgat
     5881 gtaccaaaat ccaaatcctc aagcatgaaa gattactata aggttctcgg ggtgtcgcgc
     5941 gacgcctctt tcgaggacat taaaagagca taccgaaaac tcgcccataa gtaccacccg
     6001 gacattaaag gcggcgacga ggagaagttc aaagagctga acgaggcata ccagattctt
     6061 tcggattcag acaaacgctc ccagtacgat aagttcggca gagtattcga cggccaggcc
     6121 ggggcagacc ctggatttgg aggttttagg tgggcatggg gcactcccgg ccagcaggca
     6181 gactttgagg atagcgctgc tggttttggc tttgactttg aagatattgg agacgttttc
     6241 gaggaattct ttggcgggca aacgagagac acaagaaagc aggaagtaaa gaaaggaaga
     6301 gacgtagagg tggaattgca aattccgctc gaggccacct tgagaatcag caaggaaaaa
     6361 atctttcttt cgaaatttgc ggtttgccag aggtgccagg gagtgggggc ggaacctaac
     6421 accaaggtaa cggaatgttt tgcgtgccgc ggccagggag aggtgcagca gattaagcgc
     6481 acggtgtttg gttcgtttac gcgcgtgggc acatgccctg aatgcgcagg cgaaggtttg
     6541 aaaccggaaa aaccctgcaa cgtgtgtaag ggcgaaggca gagtgaaaag cgaagaacaa
     6601 ctcgaagtaa ccattccggc aggcgttgat accaaccaga ttttgcgcgt agaaggaaaa
     6661 ggggatgccg gcagaagaaa aggcaaagcc ggcgacctgt acattcggat tgtggtaaaa
     6721 aagcacccgt tgtttgagcg gaggggagat gatgttgtgc tcaaaaaaga tattgc
//
LOCUS       DASRUP010000032         5975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_519682, whole genome shotgun sequence.
ACCESSION   DASRUP010000032 DASRUP010000000
VERSION     DASRUP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519682"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFE94_01340"
     CDS             <1..827
                     /locus_tag="VFE94_01340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX49778.1"
                     /translation="GEASLLLAHLRGKKVILTYHSDIVRQRAILPFYGPLLKRVLSRA
                     DAIIVTSPNLRDSSPFLRHVKEKCRVIPLGIDIAAFQERRAIPVTFPNPQRKKIVLFV
                     GRLVYYKGLEYLLCAMQKVDALLLIVGEGPLKEKLQNQATTLGVAEKAVFLGRISDEE
                     LKHCYEISDVFVLPSVERTEAFGLVQLEAMLAGLPVVNTSLSTGVPFVSVHGETGLTV
                     PPRDASALAGVLQKILQNPTLAENFGRKSRERVREMFSEKEMIQKIQKLYQEVLAK"
     gene            complement(832..2139)
                     /locus_tag="VFE94_01345"
     CDS             complement(832..2139)
                     /locus_tag="VFE94_01345"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZX49779.1"
                     /translation="MFRAALLVGVDIAILYAVLWSVLWFRFGDPLASGAWGQHVLPFS
                     LLYGLWLLVFHAFGLYNLGLTKNSFAFLFALSRSLGACFFLAVIFFYFTPFFGIAPKT
                     NLFLVTLLAFLFLGLWRLLYNMQARHPYHIQSIIVIGQNAHASELQEHLAQNPQFGYK
                     ISAIIRPEEQELNLLSLSKEHTILAAFDLHAYPKLTQTLFSFVPELWFESLASFYERI
                     TGKVPLSQIDEIWFLHNLRERERSAYETLKRITDILSGLVLGLFALLFLPLVAIALLL
                     ESPGSLFYAQQRVGKNGKVFTLWKFRSMVPNAEENGAAWAQKNDPRVTKAGNILRKTF
                     IDELPQAINILKGDMSLVGPRPERPEFVQSLSEEIPFYQIRHLVKPGITGWAQVNSPY
                     GNSTQGALEKLQYDLYYLKNRSLMLDLDILLKTVNVVFKGGTQ"
     gene            complement(2139..2624)
                     /locus_tag="VFE94_01350"
     CDS             complement(2139..2624)
                     /locus_tag="VFE94_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZX49780.1"
                     /translation="MPQNPNQFDVDEQIDSPTGKEELSPEIKKIAITEEAPDEHVEKF
                     HPDDWFQPEGELAIDVYQTDRDIVIQTAVAGVRTEELDIAIENDVVTIRGVRKNPNEQ
                     EEKEYFHQECFWGPFSRQVFLPEEIDVKNVDAAMKDGVLTLRLPKMEREKSKKVKIAK
                     K"
     gene            complement(2599..3321)
                     /locus_tag="VFE94_01355"
     CDS             complement(2599..3321)
                     /locus_tag="VFE94_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HZX49781.1"
                     /translation="MTYLLLFLFALAPSLIWLAFYLRKDSHPEPNRMVLKIFLAGMLI
                     TIPAIAIETFLEGFFVSFEFSRLGFLLIYFFVGIALVEELLKYVVVRLGVFSNSALDE
                     PLDFMLYMVIAALGFAAFENILLLFKLVQLYPASDIFLVNTVRFLQAIFLHALVSALL
                     GYFLALSFFQSKYRFLLFLAGISCATALHGSFNLYIFTIGDQGLLYLFLPLIPLFALA
                     MFISFGFQQLKKQTYATKPQPI"
     gene            complement(3318..5471)
                     /locus_tag="VFE94_01360"
     CDS             complement(3318..5471)
                     /locus_tag="VFE94_01360"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZX49782.1"
                     /translation="MNDLPKAYNSKEVEDKTYELWEKSGFFNPENCVKAGVTKPDGEV
                     FSIVLPPPNVTGTLHMGHAAMLAIQDILIRYHRMRGHRTLWLPGTDHAALATQSKVEA
                     ELYKKEKKTRHDLGREEFLARVEQFAKESHDTIIHQLRKMGSSLDWSREAYTLDEKRN
                     VAVRTAFKKMYEEGLIYRGARIVNWDPKMQTTVSDDEVEYKEEKGNFYYLKYGPFTIG
                     TARPETKFGDKYVVMHPDDARYQDYTHGQKIELEWINGPITATVIKDGAVDMEFGTGV
                     MTITPWHDATDFDIAERHNLDKEQIIDANGKLLPIAGDFAGMHIAKARPAIVEKLKGK
                     GLLEKIDENYTHRLATNSRGGGVIEPQIKEQWFIDVNKKIPGREVSLKELMRDTVESG
                     KIKILPERFEKIYYHWIDNLRDWCISRQIWYGHRVPVWYKGEKMYVGVEPPEGQEWQQ
                     DPDTLDTWFSSGLWTFSTLGWPEETEDLKVYHPTSVLETGYDILFFWVARMILMTGYL
                     LNDVPFRTVYLHGLVRDDQGRKMSKSLGNIIDPLDMIEKYGADATRLSLIIGAAPGND
                     VKLSEDRVRGYKHFANKVWNASKFVMQNTEDMDLSRQPELTPEDQQSLDELKALGQEI
                     TQDIENYRLYLAAEKLYHYFWHSFADVIIEQAKPVLRGEDEKAKASAQWTLHTVLATC
                     LKLMHPFCPFVTEEVYRHLHTPDKQDTLMVEKWPA"
     gene            complement(5495..>5975)
                     /locus_tag="VFE94_01365"
     CDS             complement(5495..>5975)
                     /locus_tag="VFE94_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49783.1"
                     /translation="AVAAAAALAGKPAIGIKLPRPMMHTKDYDFSFSGLKTAVLYDYK
                     ARPDQERQSKEYVQEMAAEIQQSVIDVSLHKTMKATEEFGAKSIILGGGVSANDELRK
                     QLGQKAKDLGIELLAPPKNLSTDNGLMIALAGYFTYKADSYEKNFEKIEATPNLRIA"
BASE COUNT         1477 a         1354 c         1518 g         1626 t
ORIGIN      
        1 taggtgaagc ctcgctttta ttggctcatt tgcgcggcaa gaaagttatt ctcacatatc
       61 atagcgacat tgttcgtcaa agggcaatac tgccgtttta tggccctttg ttgaaaaggg
      121 ttctttctcg ggcagatgcc ataattgtaa cctctcccaa ccttcgggat tcctcgccgt
      181 ttcttcgaca tgtaaaagag aagtgcagag ttattccgtt ggggattgat attgcggcat
      241 tccaagagag aagagcgata ccagttacgt ttccgaatcc gcagcgcaaa aagattgttc
      301 tttttgtggg caggctggta tattacaagg ggttagaata tcttctctgt gcgatgcaaa
      361 aggttgatgc attgctgctt attgttggag agggcccact taaagaaaag ctgcaaaacc
      421 aagcgacaac ccttggagtc gcagaaaaag ctgtgttctt gggaagaatt tctgacgagg
      481 aacttaagca ctgttatgag atctcggatg tatttgtgct cccttctgta gaacgcaccg
      541 aggcttttgg tttggtgcag ttggaggcaa tgctggctgg tttgcctgtg gtaaatacca
      601 gtctttccac gggggtgcct tttgtaagcg ttcacggcga aaccggactc actgtgccgc
      661 cgagagatgc gagcgctttg gccggagttc tgcaaaagat cctgcaaaat cccactttgg
      721 ccgaaaactt cggaagaaaa agccgagaaa gagtacgcga aatgttctct gaaaaagaga
      781 tgattcaaaa aatacagaaa ttgtaccagg aggtgctggc aaaataaaga tttactgcgt
      841 gccgcccttg aagactacgt tgacggtttt caaaagaata tcgagatcta acattaaaga
      901 acgatttttg aggtagtaca ggtcgtactg cagcttctcc aaagcccctt gggttgaatt
      961 tccgtacggc gagtttacct gcgcccagcc ggtaatcccc ggttttacaa gatggcgaat
     1021 ctggtagaaa ggaatttctt ctgaaaggct ttgtacaaat tcggggcgct cgggccgggg
     1081 cccgacaaga ctcatatctc ctttgagtat attgatagcc tggggaagtt cgtcgataaa
     1141 tgttttgcga agtatgtttc ctgccttggt aacgcgaggg tcattttttt gcgcccaggc
     1201 ggcgccgttt tcttcggcgt ttggcaccat ggagcggaat ttccaaaggg taaacacctt
     1261 tccgttcttg cctacccttt gctgcgcata aaaaaggctg ccggggcttt cgagcaaaag
     1321 ggcaattgca acaagcggaa gaaataaaag agcaaaaagc ccgagcacga ggccagagag
     1381 aatgtcggtt atgcgtttga gcgtttcgta tgcgctgcgt tctctttctc taaggttgtg
     1441 taagaaccag atttcgtcga tttgagatag ggggactttc ccagtgatac gttcataaaa
     1501 agaggctaag ctttcaaacc aaagttcggg tacgaaagaa aagagggttt gggtaagttt
     1561 ggggtatgcg tgcagatcga aagctgcaag aattgtatgt tctttgctaa gagaaagtag
     1621 gttgagctct tgttcttcgg gccgaataat tgcagagatt ttgtagccga attgggggtt
     1681 ttgcgccaag tgttcctgga gttccgaagc gtgagcattt tgcccgataa cgataataga
     1741 ctgtatatgg taggggtgcc gggcttgcat gttgtacagc aagcgccaaa gccccagaaa
     1801 cagaaacgcc agaagagtta ccaaaaaaag gtttgtcttg ggtgcgatgc caaagaacgg
     1861 ggtgaagtaa aaaaagataa ccgcaagaaa aaaacacgcc cccaggcttc gagagagcgc
     1921 gaaaagaaag gcaaagctgt ttttcgtgag gcccaggtta tacaggccaa aagcgtgaaa
     1981 gacgagcagc cacaagccgt acaaaagaga gaaagggaga acgtgctggc cccatgcgcc
     2041 agacgccaaa gggtcgccaa accggaacca caacacgctc cacagcacgg cgtacaggat
     2101 agcaatatct actcccacta gcaacgccgc gcggaacatt attttttggc aatctttacc
     2161 tttttgcttt tttcgcgctc cattttaggg aggcgaagag tgagcacgcc gtctttcatg
     2221 gcagcgtcca cgtttttaac gtcgatttcc tcgggcaaaa atacctgccg ggaaaagggc
     2281 ccccagaagc actcttggtg aaagtactct ttttcttcct gttcgttggg gtttttcctg
     2341 acgccgcgaa tggttaccac atcgttttct atggcaatat cgagctcttc ggttcttacg
     2401 cccgccaccg ccgtctggat aacgatgtcc ctgtcggttt ggtagacgtc aatggcaagt
     2461 tcgccctcgg gctgaaacca gtcgtcgggg tgaaactttt ctacatgttc atcgggagct
     2521 tcctccgtaa tagcaatttt tttaatctcg ggagaaagtt cttctttgcc agtcggggaa
     2581 tcgatttgtt cgtcgacgtc aaattggttg gggttttgtg gcatatgttt gttttttaag
     2641 ttgttgaaat ccaaaggaga tgaacattgc tagggcaaag aggggaataa gaggaaggaa
     2701 taaatacaaa agcccctggt ctcctatagt aaatatatac aaattaaacg atccgtgcaa
     2761 ggcggtggcg caagatatgc ctgccaaaaa cagcagaaag cggtatttgc tttgaaagaa
     2821 agacagcgcc aagaaatacc caagcagggc cgagaccagt gcgtgcagaa agatggcctg
     2881 aagaaaccgc accgtgttta cgagaaaaat gtcggaggca ggatacaatt gcacgagctt
     2941 aaacagcagg aggatgttct cgaaagccgc aaatccaagc gctgcaatta ccatatacag
     3001 cataaagtcg aggggctcgt cgagcgccga attcgaaaat acccccaggc gcactacaac
     3061 atacttgagt aactcttcta caagcgcaat gcccacgaaa aagtatataa gcaaaaatcc
     3121 gaggcgggag aattcgaaag aaacaaaaaa tccttccaaa aacgtttcaa tggcaatggc
     3181 gggaatggta ataagcattc cagccaaaaa aatcttgaga accattcggt tgggttccgg
     3241 gtgggagtct ttgcgaaggt agaaagcgag ccagattaag cttggggcca gggcaaaaag
     3301 gaaaagaagg aggtatgtca tgccggccac ttttctacca tgagcgtgtc ttgcttatcg
     3361 ggcgtatgga ggtggcggta tacttcttcg gttacaaacg ggcaaaatgg atgcatgagt
     3421 tttaagcacg tagccaacac agtatgcaaa gtccactgcg ccgaggcctt tgctttttcg
     3481 tcttcccccc gcagcacagg ctttgcctgt tctattataa catctgcaaa cgaatgccaa
     3541 aagtagtggt atagtttttc tgcggccaag tataagcgat agttttcgat gtcttgggtt
     3601 atttcttggc caagggcttt gagctcatcc aggctttgct ggtcttctgg ggtgagttca
     3661 ggctgtcggg aaaggtccat gtcttcggtg ttttgcataa caaactttga ggcattccat
     3721 actttgttgg caaaatgctt gtagccgcgc accctatctt cggaaagctt tacgtcgttt
     3781 ccgggagcag ctccaataat caaagaaagg cgcgtggcgt ctgccccgta cttttcaatc
     3841 atgtccaaag ggtcgataat gtttcccagg cttttgctca ttttgcggcc ttggtcgtct
     3901 ctaaccaatc catgcaaata caccgtgcgg aacggcacgt cgtttagcaa ataccctgtc
     3961 attaaaatca ttctcgccac ccagaaaaac aaaatgtcgt atccggtttc cagcaccgat
     4021 gtcgggtggt aaacttttaa atcctctgtt tcttctggcc agccaagcgt cgagaacgtc
     4081 cacaaaccag aagaaaacca ggtatcgagc gtatctggat cctgctgcca ctcttggcct
     4141 tcgggcggtt ctaccccaac atacatcttc tcgcccttat accatacggg gactctgtgg
     4201 ccgtaccaaa tttggcggga aatgcaccag tcgcgcaaat tgtctatcca gtggtagtaa
     4261 attttctcaa agcgctcggg gaggattttt atttttcccg attctaccgt gtctcgcatt
     4321 aattctttta agcttacctc gcggcccggg atttttttgt ttacatcaat aaaccactgt
     4381 tctttaattt ggggttcaat aacgccgccc ccgcggctgt tggtggccag gcgatgggta
     4441 tagttctcgt ctattttttc caaaagccct tttcctttga gtttttctac aatggcaggc
     4501 ctggcttttg caatatgcat gcctgcaaag tcgccggcta taggcaagag ctttccgttt
     4561 gcgtcaataa tttgttcttt gtcgagatta tggcgttctg caatatcaaa gtcagtggcg
     4621 tcgtgccagg gggtaatggt cattactccg gtaccaaact ccatgtcgac agccccgtct
     4681 tttattaccg tggcggttat cggtccatta atccattcca attcaatttt ttggccgtgt
     4741 gtgtagtctt ggtatcttgc gtcgtctggg tgcattacca catacttgtc gccaaacttg
     4801 gtttcggggc gcgcagtacc aatggtaaat gggccgtact ttaggtaata aaaatttccc
     4861 ttttcttctt tgtattctac ttcgtcgtca gacacggtgg tttgcatttt tgggtcccag
     4921 tttacaatgc gggctccgcg gtaaattaag ccttcctcat acattttttt aaacgccgtg
     4981 cgtacagcca cgtttcgctt ctcgtcgagc gtgtacgcct cccggctcca gtctaaagaa
     5041 gaacccattt tgcggagctg atgaataata gtgtcgtggc tttctttggc aaactgctcc
     5101 acgcgcgcca aaaattcctc gcggcccaag tcgtggcgag ttttcttttc ttttttgtag
     5161 agttcggctt ctaccttcga ctgcgtggcc aaagccgcgt ggtcagtacc cggcagccac
     5221 aaggttctgt ggcctctcat tctgtggtag cggattaaaa tatcctgtat ggccagcatg
     5281 gcagcatgcc ccatgtgcaa agtgcccgtt acattgggtg gggggagtac gatagaaaac
     5341 acttcgccat ctggtttagt taccccagct tttacgcaat tttccggatt aaaaaaaccg
     5401 ctcttttccc agagttcgta tgtcttgtct tctacctctt ttgagttata cgccttgggc
     5461 aagtcgttca taaacacctg tatggtagca taatttacgc gattcttaaa ttcggggtgg
     5521 cttcgatttt ttcgaagttt ttctcgtagg agtctgcctt gtaggtaaag tagccggcga
     5581 gggcgatcat taagccgttg tcggtggaga gattttttgg aggagcgagt agttctatgc
     5641 ccaaatcttt tgctttttgg ccgagttgtt tgcggagttc gtcgtttgcc gaaacgccac
     5701 cgcccaagat aatacttttt gcgccgaatt cttctgtggc tttcatagtt ttgtggagtg
     5761 agacgtcgat aactgactgc tggatttctg cggccatttc ctggacgtat tctttggatt
     5821 gccgttcttg gtctggccgt gccttgtagt cgtagagcac cgcggttttc aggcccgaaa
     5881 aactaaagtc gtagtctttg gtatgcatca tgggccgcgg cagtttgata ccaatggctg
     5941 gcttgcccgc caaagccgcc gcagcggcga cggcg
//
LOCUS       DASRUP010000033        10228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1624849, whole genome shotgun sequence.
ACCESSION   DASRUP010000033 DASRUP010000000
VERSION     DASRUP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624849"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFE94_01370"
     CDS             complement(<1..620)
                     /locus_tag="VFE94_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49784.1"
                     /translation="MKRNNFFYLFTIGLGMSLAGILVAASFTFLLQQPVEAGGADNVS
                     GWAWSSNIGWISFNCTNPGAGGCASNYGVNVDSGTGNFSGYAWAATNDPDSAIGWISF
                     NEAGPTPDGFSCGANCTAKLDLGTKKVAGWARALANGGGWDGWIKLRDDIDGDSVDDY
                     GVKWNSLAGELEGWAWGGEVMGWIAFNKELAGVPAHGGADHAVRLG"
     gene            complement(627..1589)
                     /locus_tag="VFE94_01375"
     CDS             complement(627..1589)
                     /locus_tag="VFE94_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49785.1"
                     /translation="MKHFIFHFFFAAAVFLLAVGGSVWAQWSGPSDIPPNANVPAPIN
                     VSGTAQTKGGDLTIFDPNVGINALLTTEALDVLNILNVASTMVFDGTGITNQSDILRI
                     DGPVSTSGTLEGADVSIWNNLSANGASIIGGRFESNHNSPSYSIGDIIGVRGRGYQQQ
                     GSATNVYGGRFEGFAAGLDSATNVYGVYGGANTDVASVTNAYGLYSHVGQTTSGIVNG
                     YGVYIADVAGTNDYALYAAGANDESFFAGNVTVQGKLNASGGVDPPYISFSAESHESI
                     REYAKEVDEHEKVMQFWNGKAHRLEIYVVEEDAFYTLGGELIKE"
     gene            complement(1597..1878)
                     /locus_tag="VFE94_01380"
     CDS             complement(1597..1878)
                     /locus_tag="VFE94_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49786.1"
                     /translation="MQKRDLFIIFFLGLTLVMVIWVASMFVQTKQEDERAQQETQPRS
                     EILKKANTDNQFTPEELEARREALKESGAGQQLTPEELEGRKTMLKNQN"
     gene            complement(1894..2541)
                     /locus_tag="VFE94_01385"
     CDS             complement(1894..2541)
                     /locus_tag="VFE94_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49787.1"
                     /translation="MQSYKLPILISLFFVGAVTLLFGFMSVFQKESGPQPSEPTLQDD
                     QEPVVQDPSLLQNALPQFPEGTFSDSELIEDISISIQQAENKSSTARLAWDEARFRVF
                     AVSLFDGDKIERRENDDILLWFISSFRGLPEDGQPVQRQDVTGFLSSGYVIGEGRAGF
                     DYLEAPEEFQPYTLELESGTTYYLKMVGFGGDNDANITVNKIFTFTDSCLPPNCE"
     gene            complement(2543..3421)
                     /locus_tag="VFE94_01390"
     CDS             complement(2543..3421)
                     /locus_tag="VFE94_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49788.1"
                     /translation="MKRYFSPYSFSVLVGAVVVALLTGWYAFAFTPPQDTPPTCNTGD
                     PGCDAPVNVGGTLQHKQGTLQVDYLKSHSDVDVADNITADGGSVLIFDSSVNKIDRGV
                     LPFNQGDLIAPGDRPLISGLAGYLTNYFSIANLGISGTSASNVVNGVSFGPGNSIAGT
                     ASVGQPLLPHDVVAAGGVQVTYPNSFCNYYVARWRRCVSDWGYTQPQFYGVGWYDPRG
                     VLNLSVATGITYAGCYSDRSCSTLKEHTGWATFQCNAPYRTSTSNAITGVYNGYCASG
                     ARGVRSCLQSITCHVQ"
     gene            complement(3439..3666)
                     /locus_tag="VFE94_01395"
     CDS             complement(3439..3666)
                     /locus_tag="VFE94_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49789.1"
                     /translation="MQKRDIIVVLSIVIILALVIVLWFVYSGQPQEEPIEQQGELSRE
                     ERKELLRQSAAAGEMLTEEEREQRKNQLKSL"
     gene            complement(3674..4447)
                     /locus_tag="VFE94_01400"
     CDS             complement(3674..4447)
                     /locus_tag="VFE94_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZX49790.1"
                     /translation="MAAWHIGVFIGGLLVGSFLNAVIYRLEKNESALKGRSYCPRCKH
                     QLAWDDLIPLVSFALLGGRCRYCKGRISPQYPIVEIATALLFAAFFVLQTEHVFYLLY
                     LWVVTSLLIVLFVYDLRHYILPDKILLPAIGIVLGYRVFEVWGLGFAWDLRFVVSGFA
                     AALFFFAIFALSRGRAMGFGDVKLALCMGLFLGWPNIAVALFAAFFAGAVAGIALILF
                     KKKGMKSEVPFGPFLIAGTFVALFWGKLIVDWYLDLLLL"
     gene            complement(4447..4899)
                     /locus_tag="VFE94_01405"
     CDS             complement(4447..4899)
                     /locus_tag="VFE94_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZX49791.1"
                     /translation="MLYRKAEKGFTLIELLVVIAIIGLLAGIVLVSLGGARDSARDAR
                     IQSAISETRTMAELIRLGSPFDSYSPLCAAGTLNEGHATYGQQLGFLENDIQSQSGSL
                     PDCFAVDVVYCVSAPAATSGLDDFCADRTGTIGTIAGGCTAAGDTCTP"
     gene            complement(5046..5480)
                     /locus_tag="VFE94_01410"
     CDS             complement(5046..5480)
                     /locus_tag="VFE94_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014950402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZX49792.1"
                     /translation="MNLKNKKGFTLIELLVVIAIIGLLAGIVLVSLGGARDSARDARI
                     QGAMQQTKSIAELVYNNASPLVYDSLCAGGALNGAHAVYGPQLTNLAADISVQNGGTA
                     ATCNAGGDEYCVSAPLASTGVICVDATGNTGTTACGVATACP"
     gene            complement(5594..6805)
                     /locus_tag="VFE94_01415"
     CDS             complement(5594..6805)
                     /locus_tag="VFE94_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZX49793.1"
                     /translation="MKFAYRAKDDKGRHQEGIIQAASDEAALHALDRYGFYVTWLREE
                     KEEKALFSRQIRLFERISLKDVMLFSRQLSILFRSQISLVDALGTIGTQMKHRAFREK
                     ILQISQEVDGGTALSDALAQHPKVFSPFYINIVKRGEALGNLADVLNYLADHLEREHS
                     LHSKIKGAMTYPIFVLIVAAVVISLLSVLVLPNLTKVLTDSGQELPLVTQIVISSSTF
                     YAQWWWLVSLGAVALVIGMVMFVKSKRGKSIFQRVILQIPRIGSFAKMIYVTQFGENL
                     STLIAGGVPIVEALAITGRIVGNEVYQKIIGEAKDSVAQGNRISEVLSRYPGEFPPIF
                     TQMIQVGEKSGALDTTLKEIVSFYQKELERNIDALLSLIEPIMIVALGLLVGGLMASL
                     MLPLYQGITQI"
     gene            complement(6820..7908)
                     /locus_tag="VFE94_01420"
     CDS             complement(6820..7908)
                     /locus_tag="VFE94_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZX49794.1"
                     /translation="MKFQSPMLAKNRELNELLDITIEEQASDLHLSVGQPPILRIAGE
                     LVPLSKKAPLGREILEAYVFALLTDAQKVRLQEQKELDFSYTLEDRARFRGNAFYALG
                     SLSLALRLIPHEIRGVEQLNLPSILHQFSRANQGFVIVAGPSGHGKSTTLAALIDEIN
                     HTRGEHIITIEDPIEYLFSDDKSIVDQREVTKDTHSFANALRATFRQDPDVIMVGEMR
                     DPETIGTALTAAETGHLVFATLHTNSAAQSVHRIVDSFPAEQQGQVRAQLSGSLLGVI
                     SQRLLPRLQGGLVPACEVMLANSAVSNLIRENKVHELPLVIETSAEDGMISLNRSLAT
                     LVKVREITLDSALKHSLNPNELRSLIHG"
     gene            complement(7905..8267)
                     /locus_tag="VFE94_01425"
     CDS             complement(7905..8267)
                     /locus_tag="VFE94_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX49795.1"
                     /translation="MKKVLLVEDDPLLIDIYTTKLKEAGFQVTVVEHGQKVLSALEKE
                     RPDVVLLDIVLPHMDGWEVLRAIKANDSLKEIPVVILSNLGQKEEINKGLQLGAARYF
                     IKAHYTPSQIVEELATII"
     gene            complement(8293..10062)
                     /locus_tag="VFE94_01430"
     CDS             complement(8293..10062)
                     /locus_tag="VFE94_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZX49796.1"
                     /translation="MDILELLLAKHILKKEDIQLVEKELQSSGKKAEELLVQKRRVDE
                     DLLFQVKSEALGMPLRQVEASEIPLKVLELIPEDSAKYYKMVPLVSSRGRLEVGMVYP
                     EDAKAQEALQFLARQGNFSYTVSLITLSTFEQLMKQSRNLKSEMTKALQELEQEIGKG
                     EEGKAAAPETGQVIEDAPVTKMVAVILRNAVEGGASDIHIEPGREALRVRFRFLGDLH
                     ASLMLPSKVSQAIVARIKILSNMKIDETRKPQDGRFSAQISGRSIDFRVASLPTPQGE
                     KVAIRVLDPTTGFKSFEDLGVEGRNLEKIKRAAEKPFGLILVTGPTGSGKTTTLYAVL
                     RHLNKETVNILSLEDPVEYFIEGVNQSQVRPEIGYDFAQGLRQLLRQDPDIIMVGEVR
                     DKETASLVIHASLTGHLVLSTLHTNSAAGVIPRLIDMEIDPYLIPPTLTLAIAQRLVR
                     RLCNECKEKVEPERGMREIIWKEIQQLPALAKKELPPKTGDSPGQIKTCKAVGCKKCG
                     ASGYSGRIALMEVLEMSDAMIELIGESVSEAKIAQLAQKEGMITMRQDGVLKVLDGVT
                     TIEEVLRATTETSVEPEEEKKEE"
BASE COUNT         2615 a         2821 c         2244 g         2548 t
ORIGIN      
        1 ctccccaacc gtaccgcgtg gtcagcgccg ccatgcgccg gaaccccggc aagctcttta
       61 ttaaacgcta tccaccccat tacctcccct ccccatgccc agccttccaa ttctcccgct
      121 agcgaattcc attttactcc gtaatcgtcg acgctatcgc cgtcaatgtc atcacgaagc
      181 ttaatccagc cgtcccaccc gccgccgttt gccagcgcgc gtgcccagcc tgcaactttc
      241 tttgttccca ggtcgagttt tgcagtacag tttgctccgc acgaaaatcc gtctggcgtc
      301 ggcccggctt cgttaaaact aatccagccg atcgccgaat cagggtcgtt tgtcgcggcc
      361 cacgcatagc cggaaaagtt accggttccc gaatctacat taaccccgta gttgcttgcg
      421 caaccaccag caccagggtt cgtacagtta aaacttatcc acccgatgtt agaactccac
      481 gcccagccag acacgttatc tgcaccgcct gcttctaccg gctgctggag taaaaacgta
      541 aagcttgccg ccaccagaat gccggccaaa ctcattccta gcccgatcgt aaatagatag
      601 aaaaagttgt ttctcttcat aaaaaattat tccttaatta gttctcctcc aagagtataa
      661 aacgcgtctt cttccaccac atatatttca agcctatgcg cttttccgtt ccaaaactgc
      721 atcacttttt cgtgttcgtc cacctccttt gcgtattctc ggatactttc atgcgattct
      781 gccgagaaag aaatataggg aggatctacc ccgcccgaag cgtttaactt tccctgtacc
      841 gtcacattcc cggcgaaaaa agactcatcg ttagcccctg cggcgtaaag agcgtagtca
      901 ttcgttcccg ccacgtccgc gatgtacacg ccgtaaccat taacaatccc cgaggtagtt
      961 tggccgacgt gagagtacaa tccgtaggca ttggttaccg aggccacatc agtattggcg
     1021 cctccatata ctccatatac attggtggcg ctatccaacc ccgccgcaaa tccctcgaat
     1081 ctccctccat atacattcgt cgccgagcct tgttgttgat accccctgcc gcgcacgcca
     1141 attatatcgc ctatcgaata tgaaggcgaa ttatgattgg attcaaacct cccccctatg
     1201 atcgatgctc cattcgccga aagattgttc caaatcgaga catccgcgcc ctccaaagta
     1261 ccgctcgtgg aaaccggccc gtcaatgcgg agaatgtctg actggttggt aatgccagtc
     1321 ccgtcgaata ccatggtgct cgcaacattt aagatattga gaacatccaa agcttccgtg
     1381 gttaaaagag cgtttatccc gacattggga tcgaatattg taagatcccc ccctttggtt
     1441 tgagcggttc cgcttacgtt aattggcgct ggcacgtttg cgtttggcgg aatgtcggaa
     1501 ggacccgacc attgcgccca taccgatcct cccaccgcga gcaggaaaac tgcggcggca
     1561 aagaaaaaat gaaaaataaa atgcttcatg atattactaa ttttgattct ttaacattgt
     1621 ttttcgacct tccaattctt ccggagtcaa ttgctggcca gcgccagatt ccttcagagc
     1681 ttcacggcga gcctccaatt cttcgggggt gaactggtta tccgtatttg cttttttgag
     1741 gatttcgctt cgcggttgag tttcttgctg cgccctctca tcttcttgct tcgtttggac
     1801 aaacattgaa gcaacccaaa taaccatcac cagcgtcaac ccgagaaaga atatgataaa
     1861 taaatcgcgt ttttgcatac tgttattata tttttactcg cagtttggag gaaggcatga
     1921 atcagtaaac gtgaatattt tattcaccgt aatattggcg tcgttgtctc ctccaaaccc
     1981 aaccatcttg aggtaatatg ttgtaccaga ctccagctcg agcgtatacg gctggaactc
     2041 ttcgggagcc tcaagatagt caaaaccagc tcttccctcg ccaattacat accctgaaga
     2101 gagaaaccct gttacgtctt gcctttgaac cggttggcca tcctctggga gccctcggaa
     2161 agaagaaata aaccagagga gtatgtcgtc gttttctctc cgctcaatct tatctccatc
     2221 aaacaacgaa acagcaaata cgcggaaccg agcttcgtcc catgcaagcc tggcagtgct
     2281 ggatttattc tctgcttgtt ggatactaat gcttatgtct tcgataagtt cggaatcaga
     2341 aaacgttcct tcggggaatt gcgggagggc gttttggagt aaagaaggat cctggacaac
     2401 gggttcctgg tcgtcctgca aagttggttc agacggctgc ggcccgcttt ctttttgaaa
     2461 taccgacata aagccaaaaa gaagcgtaac cgcgcctacg aagaaaagag aaataagaat
     2521 gggaagtttg taagactgca tattattgta catggcatgt aatgctttga aggcatgaac
     2581 gcacaccgcg cgcaccggaa gcacagtatc cgttgtatac acccgttatt gcgttgctcg
     2641 ttgaagtacg ataaggagca ttgcactgaa aggtagccca ccctgtatgc tccttaagcg
     2701 tagagcaaga tctatcgcta taacagccag cataggtaat tccagtcgct acgctcaagt
     2761 taagcacacc tcttggatcg taccaaccaa cgccatagaa ctggggctgg gtataacccc
     2821 agtccgatac gcaacgcctc cacctggcca cgtagtaatt gcaaaaacta ttagggtatg
     2881 taacctgaac gccgccggcg gccacaacat catgcggaag caatggctgc ccgacactcg
     2941 cggtgcccgc tatgctgttt cctggcccaa aactcactcc gtttactaca ttcgaagctg
     3001 acgtacctga aattccaagg ttcgcaatac taaagtaatt agtcagataa ccagcaagcc
     3061 ccgaaatcag cgggcgatcc cccggggcta taagatcgcc ctgattaaaa gggaggactc
     3121 cccggtctat cttgttaacg ctgctgtcaa agattaacac ggagcctccg tctgctgtta
     3181 tgttgtctgc cacatccacg tccgaatgtg atttcaaata atcgacttgt agcgttcctt
     3241 gtttgtgctg cagcgtccct cccacgttga ccggggcatc gcatcccgga tctcctgtgt
     3301 tgcaggtggg cggagtatct tgcggaggag taaacgcgaa cgcgtaccac cccgtgagaa
     3361 gcgccacgac cacggcgccc accagcacag aaaaggagta gggagagaaa tatcttttca
     3421 taacgaaaca gttacttgtt atagcgactt gagttgattt tttctttgct ctcgctcttc
     3481 ttccgtcaac atttctccgg cagcagccga ctgccgcaac agctcttttc gctcttctct
     3541 cgaaagttca ccttgctgct ctatcggttc ctcttgcggt tggccagaat acacaaacca
     3601 cagcactatc accagcgcga ggatgatcac aatcgataac actacgatga tatcgcgctt
     3661 ttgcattacc ctattataac aataacaggt caagatacca atccacaatg agttttcccc
     3721 agaataacgc aacaaatgtg ccggcaatta agaagggacc aaacggaact tccgatttca
     3781 ttcccttttt cttaaaaaga ataagcgcaa tccccgctac agccccggca aaaaatgcgg
     3841 caaataacgc aactgcaata tttggccagc ccaaaaacaa gcccatacac aaagccagct
     3901 ttacgtcgcc aaaacccatg gcccggcccc tggataaagc aaagatggcg aagaaaaaca
     3961 aagctgccgc aaaacccgaa accacaaacc tcaagtccca agcaaaaccc aaaccccaaa
     4021 cctcaaacac tcgatagccg agaacaattc cgatggctgg cagtaagatt ttatctggca
     4081 aaatataatg ccgaaggtcg tacacaaaca aaacaatcaa caaactcgta accacccaca
     4141 gatataacaa atagaacacg tgttcagttt gtaatacaaa aaacgctgca aacaaaagag
     4201 cggtggctat ttcgactatc gggtattgcg gggaaattcg ccccttacaa tacctgcacc
     4261 tcccgcccag cagcgcaaaa ctcaccaagg gaattaaatc gtcccatgcc agctggtgct
     4321 tgcaccgcgg gcaatacgac cttcctttga gggcagactc gtttttctca aggcggtaaa
     4381 tcaccgcatt caaaaaactc cccacaagga gccctcctat aaaaacgcca atatgccaag
     4441 cagccattaa ggggtgcagg tatcgcctgc cgcggtacaa cctcccgcga ttgtcccgat
     4501 agttcccgtt ctgtcagcgc agaaatcgtc tagcccggaa gtggcagctg gcgcggaaac
     4561 gcaatataca acatcaacgg caaagcaatc gggaagcgaa ccgctctggc tttggatatc
     4621 gttttccaga aacccaagct gttgcccgta cgtcgcgtgc ccctcgttaa gcgtaccggc
     4681 cgcgcataac ggagaataag aatcaaacgg agatccaagc cgtattaact ctgccattgt
     4741 ccttgtttca gatatggcgc tttgaatgcg ggcgtctctt gcagagtctc ttgctccgcc
     4801 caacgaaacc aatacgattc ccgcaagcaa gccgataatg gcgataacta ccaataactc
     4861 aattaaggta aaaccttttt ctgcctttcg gtacaacata caaacattgg ccaaactcga
     4921 aacattcgac cttttgggtt gtatttatat tcttatacta tagcatattt ctccatacct
     4981 aaacagaaaa cgccccattg ccggagcgtt ttcggtttgc tagacagtaa aatctcgcaa
     5041 ctatactaag gacaggctgt tgctacacca catgcggtgg tccctgtatt tcctgtcgca
     5101 tctacgcaaa ttaccccagt cgacgcaagt ggcgcagaaa cacagtattc atcaccccca
     5161 gcattacagg ttgctgctgt tcctccattt tgaactgaaa tatcggccgc aagattagtc
     5221 aattgaggac cgtatactgc gtgcgcgcca ttaagcgcac caccggcgca tagtgagtcg
     5281 tacacaagag ggcttgcatt attatacaca agctctgcga tagactttgt ctgctgcatg
     5341 gctccctgga ttctggcgtc tcttgcagag tctcttgctc cgcccaacga aaccaatacg
     5401 attcccgcca acaagccgat aatggcgata acaaccaata actcaataag cgtaaatcct
     5461 tttttgttct tcaaattcat atcagtacta aagaattctc ataatcgacc ttttcgaatt
     5521 tccatttcta ttatagcact ccacgccaaa agcaaaaacg gtaactgtgg aaaacttttc
     5581 gagtcgaaag gtgttatatc tgggtgatac cctggtacaa aggcagcatt aacgaagcca
     5641 tcaaacctcc caccagcaac cccaacgcca caatcataat aggttcgatt aaggaaagga
     5701 gcgcatctat gttgcgctca agttctttct ggtaaaatga aacgatttct ttgagcgtgg
     5761 tgtccagggc tcccgacttc tcccccactt ggatcatttg ggtaaaaata ggggggaatt
     5821 ccccgggata gcgcgaaagc acttcggaaa tacggtttcc ctgggcaacg gaatccttgg
     5881 cttccccgat aatcttttgg tatacttcgt tgcccacaat tctgcccgta attgccaaag
     5941 cttccacaat gggcactcct cccgcaataa gagtcgagag gttttctcca aattgcgtaa
     6001 cataaatcat tttggcaaaa gagcctatcc tggggatttg aagaataacc cgctgaaata
     6061 tgcttttgcc cctcttgctt tttacaaaca taaccatgcc tataacgagc gcaaccgcgc
     6121 caaggctcac taaccaccac cattgagcgt aaaaagtcga agaactaatc actatttggg
     6181 taaccagcgg aagctcttgc cccgaatcgg taagcacttt ggtaaggttg ggaagcacca
     6241 aaaccgaaag caaagaaatt accactgccg ccacaataag tacgaagata gggtaggtca
     6301 tggctccctt aattttactg tgcaaactat gctcccgctc caagtggtct gccaggtagt
     6361 tcaatacatc ggccaaattt cccagcgctt ctccgcgttt tacgatattg atataaaaag
     6421 gagaaaatac cttggggtgc tgcgccaaag cgtcggataa ggctgtgccg ccgtccacct
     6481 cctgggaaat ctgcagtatc ttttcccgaa acgcccgatg cttcatctgg gtgccgatgg
     6541 tgcccaacgc atccaccagc gaaatctgcg accggaacaa aatcgaaagc tgcctggaaa
     6601 aaagcatcac gtcttttagg gaaatccgtt cgaaaagcct aatctggcga gaaaagagcg
     6661 ccttttcctc tttttcttcg cgcaaccatg taacataaaa cccgtaccgg tctaatgcat
     6721 ggagagccgc ttcatcggaa gcggcctgaa taattccctc ctgatgtctt cctttgtcgt
     6781 ctttggcgcg atacgcaaac ttcatagtgt cttactctct tatccgtgta taaggcttcg
     6841 tagctcgttg ggattcaagg aatgcttgag cgcgctatcc aaggtaatct cgcggacttt
     6901 cactaacgtg gcaagagacc ggttcaaaga aatcattcca tcttccgcgg aagtctcaat
     6961 aaccagggga agttcgtgca ccttgttctc tcgtataagg tttgacacgg ccgaattcgc
     7021 cagcatcacc tcgcaggcgg gaaccaaccc tccctgaagc cttggaagca aacgctggga
     7081 aataactccc agcagggagc cggaaagctg ggcccgcacc tgaccctgct gctctgcggg
     7141 gaaagaatcc acaatacggt gcacgctttg cgccgcagag ttggtatgaa gcgttgcgaa
     7201 caccaggtgc ccggtttcgg cagccgtaag agccgtgcca atggtttcgg gatcgcgcat
     7261 ttctcctacc ataatcacgt cggggtcctg gcggaacgtc gcgcgaagcg cgtttgcaaa
     7321 actgtgagta tccttggtta cctcgcgctg atccacaata gacttgtcgt cggagaacaa
     7381 atactcgata gggtcttcaa tggtaataat atgctctcct cgggtgtggt tgatttcgtc
     7441 tatgagagcc gccagcgtgg tggacttgcc gtgtcccgac gggcccgcca ctatcacaaa
     7501 tccctggttt gcccgggaga attggtgaag gatagaagga aggttaagct gctccacgcc
     7561 gcgtatctcg tgggggatca accgaagcgc caggcttaaa cttcccaaag cgtaaaaggc
     7621 gttgcctcga aaacgggcgc ggtcttctaa cgtataagaa aagtcgagtt ctttttgctc
     7681 ttgcaatctt actttttgcg cgtcggtaag gagcgcgaac acataggcct ccagaatctc
     7741 tctgccaaga ggcgcctttt tgctcaacgg caccagctct cccgcaatgc gcaaaatcgg
     7801 aggctggccc accgaaagat ggaggtcgga agcctgctct tcaatggtaa tatccaaaag
     7861 ttcgttgagt tctctgtttt ttgccagcat ggggctttgg aatttcatat gatagttgca
     7921 agctcttcta ctatctggct tggcgtataa tgggccttaa taaaatacct ggcggccccc
     7981 agctgcagcc ctttattgat ttcctctttc tgcccgaggt tcgaaaggat aaccacggga
     8041 atttctttta aggaatcgtt cgcctttatg gcccgcagaa cttcccagcc gtccatatga
     8101 ggaagtacga tgtccaagag caccacatcg ggcctctctt tctctagggc gctcaacact
     8161 ttttggccgt gctcgaccac ggtaacctgg aatcccgcct ccttcagttt tgtcgtgtag
     8221 atgtcaatca acaatggatc gtcttctacc aaaagcacct tcttcataca aataatacta
     8281 atcttactta cattattcct cttttttctc ctcctctggc tcgacagaag tttccgtggt
     8341 tgcgcgcagc acctcttcta tggttgttac tccgtcgaga actttcagca ctccgtcctg
     8401 gcgcatggtt atcatcccct ctttttgcgc cagttgcgca atctttgctt cagaaaccga
     8461 ctcgcctatc aactcaatca tggcgtccga catttcaaga acttccatta atgcaattct
     8521 tcccgaatac cccgacgctc cgcatttctt gcagccgacc gccttacatg tttttatttg
     8581 ccccggggaa tcgcctgttt ttggaggcag ctctttcttc gctaatgcgg gcaactgctg
     8641 aatctccttc cagattatct cgcgcattcc gcgttccggc tctaccttct ctttgcactc
     8701 attacacaat cttctcacaa gacgctgggc aattgcaaga gtaagcgtag ggggaatcaa
     8761 ataagggtct atttccatgt ctatcaagcg gggaatgact cctgcggcgg aattcgtatg
     8821 cagcgtcgaa agcaccaaat gcccggttaa agaggcgtgg attacaaggg aagcggtttc
     8881 tttatcgcgc acctctccta ccataataat atcagggtcc tggcgaagca actggcgcag
     8941 cccctgcgca aagtcgtacc cgatttcggg gcgcacctgc gactggttta cgccttcgat
     9001 aaaatactcc accgggtctt ccaggctcaa gatattgacc gtctccttgt tgaggtgtcg
     9061 aagaacggca tacagcgttg ttgtttttcc agaccccgta ggcccggtta ccaaaataag
     9121 cccaaagggt ttttcagccg cccgtttaat tttttcgagg tttctgcctt caacacccaa
     9181 atcctcaaag ctcttaaatc ctgtggtggg gtctaatacc cgaattgcca ctttttcacc
     9241 ctggggagta ggcagagaag ccacgcgaaa atcaatgctt cttcctgaaa tttgggcgga
     9301 aaatcttccg tcttgcggct ttctggtctc gtctattttc atgttggaaa gaatcttaat
     9361 gcgcgccaca atcgcctggc tcaccttgct gggcagcatt aaagaagcat gcaaatctcc
     9421 cagaaaacgg aaccgcacgc gcagagcctc acgcccgggc tcaatgtgaa tatcggaagc
     9481 tccgccttcc acggcattac gcagtattac cgcaaccatt ttcgtaaccg gggcatcttc
     9541 aattacctgc cctgtttccg gagccgcagc cttcccttcc tctcccttgc caatttcctg
     9601 ttcgagttcc tgtaaagcct ttgtcatctc ggactttaag ttgcgcgact gcttcattaa
     9661 ctgctcaaat gtggaaagcg tgatgagcga aacggtgtag gaaaaatttc cctgccgtgc
     9721 caaaaactgc aaagcctcct gggctttggc gtcttctggg tataccattc caacttcaag
     9781 cctgccgcgg gaagaaacca gaggcaccat tttgtagtat tttgccgaat cttcgggaat
     9841 gagttccagc actttcaaag gaatttccga cgcttccacc tgccggagcg gcatacccaa
     9901 agcctcgctt ttcacctgga agagaaggtc ttcgtctact ctccttttct gcaccaacag
     9961 ttcctcggct ttcttgccag aagactgaag ctctttttcc accaattgga tatcctcttt
    10021 tttgaggata tgctttgcta aaagaagttc caaaatatcc atagagctat actccattaa
    10081 aggaggaagg gatctcgctt gcccacttct tcgggggcag gaacaggaat agggggcctg
    10141 gcttctccca gttccaggaa agcaggatgt tggaaaacct caaagtccac gtgcacctcc
    10201 tggggagggg ggggaggatc ctggaaca
//
LOCUS       DASRUP010000034        18498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1302611, whole genome shotgun sequence.
ACCESSION   DASRUP010000034 DASRUP010000000
VERSION     DASRUP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18498
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302611"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1167)
                     /locus_tag="VFE94_01435"
     CDS             complement(139..1167)
                     /locus_tag="VFE94_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49797.1"
                     /translation="MLRKTVGKYAIIGLAGVLLVWFLYAANFVPFLMNKNLNAAIFQA
                     QQGDCQAAFQTLENIPSDTYLDSYVANQYVDVTTLCINRLAGQERNEAASQALAGLQE
                     RSQQHPHDTKTWIALGAYTTLLLSLVEDPREKTELAEAAKSYLAKAKELSPKREEVDE
                     RLVYLLILTKDYQGAKEVAQNCAERFPLNSECVAWRLVTELWLGERDSLHLGSLEPPY
                     NEDIARHFYTTNFLNQLLQFYLEQENYGQLVEVYLRLKTIDERPEYYASLALAYDKLG
                     SWEDMAIYSFKVYQLRPAAKEEVRAFLKEALAKGKTTDPVRLNELRKSSQLFRDIEQA
                     FPGIFESL"
     gene            complement(1145..2482)
                     /locus_tag="VFE94_01440"
     CDS             complement(1145..2482)
                     /locus_tag="VFE94_01440"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZX49798.1"
                     /translation="MQSSERALQNRLQLPWPFLAGIIAILILPLFSQFPYFAPPAWGQ
                     ALFFRSIVAVLLFFALAWLLQKGAERVISRTDPARAPILALLAYAGVLFLALFTSLDP
                     HFSFWGTPYRAWGLLTYAFYILFALGLYLFLRPQQWNSIWNTLFATGTAAVLIGIGQQ
                     FGLFADSLVSSESRPYATFGNTIFFAIFLSVLLPFAIVWFLKAKGWWKIAYGALSLLF
                     LYAILITETRGVFLGVAAGALFFVLFYPAPMRLLKKVALPLFALAFLLLILVNTVQPP
                     AFVEQTLLFSRLWDRLSLEQIWETEPRFGAWPYAISAIQEKPLLGYGPYNSSIPFNTH
                     FDSASSRFEGRTTGWWDTFHNYYLDIAIGAGLLGLFAFFAFIGLLWRGLQRAKKSAQH
                     KTALLDIHAAQTALLVYLVAMIFSFNSFGTYLVFFVLVGYSFFLIAYAPQNSR"
     gene            2572..3549
                     /gene="pseB"
                     /locus_tag="VFE94_01445"
     CDS             2572..3549
                     /gene="pseB"
                     /locus_tag="VFE94_01445"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HZX49799.1"
                     /translation="MSFEGKTILVTGGTGSFGQAFVKALLQDHNPRSVRVFSRSEYTQ
                     WEMAQNFPDPRLRFLIGDVRDKDRLSRAMNDVDIVVHAAALKQIVLGEYNPLEVIQTN
                     VMGSANVVDAAIDNNVEKVILISSDKAVNPVNLYGATKLCGERLFVHANVYVGENRRT
                     KFSVARYGNVVGSRGSVVPLFMKQAKSGKITVTDERMTRFWITLEQGVRFVSDCINRM
                     QGGEVFVPKIPSMRIMDLIQVLAPSSEVQFIGIRQGERLHEVLLAEEESRHSKEFDSY
                     FLIEPEFPFWKAEHGNGGNPIAGKFVYSSDSNTRWLSGEEIQAMLKDVV"
     gene            3552..4997
                     /locus_tag="VFE94_01450"
     CDS             3552..4997
                     /locus_tag="VFE94_01450"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49800.1"
                     /translation="MKWCFLVNTAPFLSDFFLKLASEIVRGGDDCLVVFTSKFAEYEK
                     GHLVPEGVRVVSFVDWCLQSPDVKEVPPRALSWKEFFSAFDRFKRARISYEDAVSRMA
                     HDYAFFSQLFRTEHIDAIFFEPPSGANTQIAYLLAKEKQIPYISLLTSKIYNHLDLLD
                     VKYTDSRYAATFARLRGEDLTPEEKKFATEYIASFMSHQKTLSYMGLQKIRFSPWSFL
                     SHYAKRAKHVGKILFRYAKIRGRARQYDFEAEARWLRSIRAPFETLRRQARIRRQASY
                     YRFADKRDTGRFFLFPLHLQPELSTSVWATYYNDQLNTIRNAAFALPFPYKLYVREHP
                     SAIGTKPNEFYEEIQKIPQAVIISPEESASQLVADSRGVIALTSTIGMEAALAGKPAY
                     VLGDAFYEYHPLCRRVASFEDLKRAIERDEHTPPDLSNLEGINMRFIASYMRNTVEGT
                     MVAAVKEDDSNDYREIYKAFRKRIEEIKALG"
     gene            5030..6859
                     /locus_tag="VFE94_01455"
     CDS             5030..6859
                     /locus_tag="VFE94_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX49801.1"
                     /translation="MRVDLKGKLDVLVKVNALLDQREKARFAGVAAAALAAGLFQALG
                     VVSILPFIELVMNPDTALKEGILALFFASFGFESIFVFTMVVGFAMLGILVAGNLVSA
                     FAEWVKIRFVWQKSHAISVALLEQYLSLPYVYFLNAHSADLSKNIVFEVQQFTTRLLM
                     PLLQIITKGILVALIIGLLLFVDPLIALGAALVFGVSYFVVFFFLRGSLRQRGERRLR
                     ENTGRFVAAGEAFAGIKDIKALGREEYFLERFSKHSDTFSKLQAWTGVAIQLPRYVME
                     IVAFGGAISLILVFLSLGQGSSEIIPLISFFAFAGYRVMPAVQSIFQAASDIQFHRAV
                     LDRFAEDMRASTPRQRVARAKELPEALAFTESIQLEGVYFVYPYAKEPAIRNINMQIS
                     KGTSVGLVGSTGGGKTTLVDILIGLLPPSKGSFKVDGVSVTEGNVRNWQRNIGYVPQQ
                     IFLADDTIARNIAFGLPDEKIDMDKIVTVCKMANLHDFIQRELPKGYHTKIGERGVRL
                     SGGQRQRIGIARALLHNPAVLVLDEATSSLDGVTEKAVLEAIGNVARFKTLVIVAHRF
                     ATVQNCDVIYFIEKGRIAASGSYNDLLKTYPRFRAMAQQPEPS"
     gene            6864..7610
                     /gene="kdsB"
                     /locus_tag="VFE94_01460"
     CDS             6864..7610
                     /gene="kdsB"
                     /locus_tag="VFE94_01460"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZX49802.1"
                     /translation="MQDRRAKTIGIIPARLASTRLLHKPLLDIAGKSLVQRSWEQALQ
                     ARLLDKVVVAVDDERVFKEVTSFGGEAMMTSVECQTGTDRVAEAARHFPEAEVIVNVQ
                     GDQPLVPPQAIDEVAKAMQENPEIEISNLVAKASREDAENPSVVKIVQDRQGFALYCS
                     RSPIPYPRNEEFCSWYKIMDVYGFRPAALQKIAALPPTSLEKAESVEQLRILEHGYKI
                     KLVETPCDFIGVNTPEDLEKVRALLSHGES"
     gene            7597..9495
                     /locus_tag="VFE94_01465"
     CDS             7597..9495
                     /locus_tag="VFE94_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HZX49803.1"
                     /translation="MGNPNTVKLLVTDVDGVLTDGKLYLDVEGARELKSFHVRDGMGL
                     LLAREAGIKTVFLSGRDSGAIRKRAQELQIDYIILGSKNKEQDLEEVLKQEGMALHNV
                     CFIGDDVQDLPLLRAVGFSASPHDAANEVRRQVDYVAEKNGGEGAVRDIVDYILKSKM
                     GFREVTERLFAIEEKMDLFNRKVAGEFFWEFVRSSVRRKILGQVGIFSRKEPPGKRSM
                     GYFVGVLLHVVQNSLLHNPFFAPKADVLFFGMNRRRLQEDGLWHDIYCDPLIEALEKE
                     GVRCCMVERPPFGRPVPRLVKTRHLYYFDFLELCYLVVRRLGFGRARLSEDERAFLAK
                     VQERLNEEFGTELNLETQLIRELEKEKILSWLYRGLLRQIAPKAVIVADLSAKAPLAA
                     ACKGLGIPLIELQQGAIHRYSLEYSYEGSKRQKRRVPDYFFAFGDFWKSHVALPLPPE
                     RVLSVGFPYYEQERKKYMHGARKNQIIFLSQPTSRGTLAEFAADLAREVGDTWHVVYK
                     LHPEEAFVWEKRYPGLANSDVEVVKDNEKPLYQLFGESRLQVGTNSTALYEGLGFGLE
                     TYLLETPEMEMMYLLDVGYARKVTSVQEMQSFLAKQENSQTNHGEEFFRSGSLGRMVT
                     LIKQIGAL"
     gene            9492..10637
                     /gene="pseC"
                     /locus_tag="VFE94_01470"
     CDS             9492..10637
                     /gene="pseC"
                     /locus_tag="VFE94_01470"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HZX49804.1"
                     /translation="MKRKHIPYGSQWIDEADITAVAKVMRGHWLTQGPAIERFEKKVA
                     AYCGAAYGVAVANGTVALHAAYAVAGIGPGDEVITTPLTFAATANAVVYCGGKPVFAD
                     IEEHTLNIDPREVEKKITSRTKAVAPVDFAGHPAEYDEIRKIAKKHKLLVIEDAAHAL
                     GSVYRGRKIGSLADMTIFSFHPVKTLTTGEGGMVLTNNKNFAEKLKMFRHHGIEKKPE
                     KGGWYYEIENLGYNYRITDIQCALGESQFKKLSAFLARRREIVRQYTNAFAGIPALIL
                     PQEKPYVQAAYHIYVVQFRLELLGADRKTLFEKLQEKGIGVQVHYMPLHLHPFYQKTF
                     GYKKGDFPRAERYYERALTLPLFPKMTDKEVKYVIRGVQKLVEQYQK"
     gene            10634..11371
                     /locus_tag="VFE94_01475"
     CDS             10634..11371
                     /locus_tag="VFE94_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HZX49805.1"
                     /translation="MKIYAIVQARTTSTRLPGKVLEEIEGKPMVWHVMNRVLAAETVD
                     KAVLAFPDTKENDMIQMLAEKHLWEYARGSEDDVLARYVSALTLFPCDHVVRITADCP
                     LADPAIIDLVVKEHLESSADYTSNTLKRDFPRGLDVEVFRVVVLEQAHREAKQPYARE
                     HVTPYIYEHPEVFLLRSVEAPKALKRPDLRLTVDTKEDLELVRAIYGHLYEPGKIFGL
                     QEVIALFHQYPDLKDINAAVKQKTLGD"
     gene            11376..12953
                     /gene="pseI"
                     /locus_tag="VFE94_01480"
     CDS             11376..12953
                     /gene="pseI"
                     /locus_tag="VFE94_01480"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HZX49806.1"
                     /translation="MNIVTIQQRTIGRGQPCFIVAELSGNHHQNYEEAVELVKLAKEA
                     GADAVKLQTYTPDTITLDSRNKWFIVEGADRPDSWQKKSLYELYQQAYTPWEWQPKLK
                     ALADELGILLFSSPFDETAVDFLEQEVEPPCYKVASYEAVHIPLLKKIAATGKPIILS
                     IGFADLEEVTLALQTLRENGATEIAVLHCVTSYAESPRIEDMNLRTIADITERFGVPS
                     GFSDNNGGVEIPVAAAVLGGASIIEKHFILDHGTGSPDARFSIDPEEFKRMVQLIRRA
                     EAGEREEVLKEIGVENAEAAMGNVHYGPANQQEEDNKQFRPGIWAKADIAKGEVFTKE
                     NIRVARPNAPDSFMPKDFDWILGRKAQADIAFANPITPEVVQRSRVRRATPQDAEAIL
                     EIRNSKEARMQSAENQEEIPLEKHIQWFQKQYFKEGAKNACYVAELENRVAGYCRFDE
                     KEDEVFEVSIAIASGFQGKGLGSLLLLESLEQFPDSKKVVAKVKKDNKASNRLFEKCG
                     FRVAKEDPLFFTYAYSR"
     gene            12937..14625
                     /locus_tag="VFE94_01485"
     CDS             12937..14625
                     /locus_tag="VFE94_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZX49807.1"
                     /translation="MRILVNAAGTIVRSSVSLQEGVNVLGRAYGRVAEHFVLQPLGQG
                     DLEQEVSPASLDASGVILFVKQGRVRWAAPDVSASRDLFYAKKESGVWELSDNFFDIA
                     SSFSQVSWRKQNLMFFLRHGYFSPGLTFFEEISRVRVGCKLVFGKEGVQEESVWESHG
                     SEDRSYEVFKKALNSVLEVRGLEDAHALLLSGGCDSGLLAAIGTKVLGKHPLSFTSVY
                     AQTLKVNEQDGFKAEKVSSHLGLEHGTLHLDLAKETVQPLYLLVEHMPLAAHFGIAFF
                     RIYEELARRGRTKVWTGQNADSVYNFGPTGRSKGGMLKRWYLSKEYWESLPDITTRKP
                     WNQVMKWGGEAGMVALRTQKGMRVRQPKTFAELLSVFENEEGSFRFPKAGSPRQEHMG
                     IGPLSYGEAKHLFFDRKIQTFLTGGDPRVHYGAADGTGSEAMLAYSAANMMAFWRGLP
                     LQLQDVFSPKRYIYRYLRELLGKKAYKNLYETPVPLSSAKPYITWAEWQEQVLESTRF
                     GKELREAIGGLSEFPFLAEIFNPHVLHHPLDAFWLLEVLEKVKKQGVHVREMIL"
     gene            14653..15414
                     /locus_tag="VFE94_01490"
     CDS             14653..15414
                     /locus_tag="VFE94_01490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZX49808.1"
                     /translation="MLRLLEKFCQRAANFVGYKIVKIEKSADPFRDPDFERLYEKCKK
                     HTMTSKGKVYALYNAVKYILQAGIPGDFVECGVWRGGNTMLIAYMLLEMQDTSRKIYL
                     YDTFEGMVEPTPNDYTFLNQIPAVLRWKREQKDAYNEWCFAPLEEVQEAMFSTGYPKD
                     RMVFVKGKVEKTIPQTMPEQISLLRLDTDWYESSRHELAHLFPLLARKGVLILDDYRY
                     WAGQKKATDEYFTETKTPMLLVDNIGGATVGVKIS"
     gene            15479..16813
                     /gene="aepX"
                     /locus_tag="VFE94_01495"
     CDS             15479..16813
                     /gene="aepX"
                     /locus_tag="VFE94_01495"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HZX49809.1"
                     /translation="MKKQAKAKKEPVVYVGMAADLIHHGHVNIIDEASKLGEVVVGLL
                     TGEAIASYKRVPFLKFEERKFVIERIKGVKEVIAQETLDYVPNLKKVKPDFLVHGDDW
                     RTGVQRETRARAIRALKEWGGKLVEPTYTSGISSTDLIKYAIEAGITPTQRLQRMRRQ
                     LAVKPLLRFMEVHNGLSGLIVENTTALRDKRKVEFDGMWGSGLTDSTAKGKPDNSVVD
                     ITSRLQSLEQILDVTTKPILVDADNGGLSEHFVHTVRKAEGLGISGLVVEDKIGAKRN
                     SLFGTDVAQEQDTIEDFAAKITAGKRSQVTDDFMIIARIESLILKKGQKDALARAHAF
                     IEAGADGIVMHSKEKTPAEILAFCKEYKKFTNRVPLVVIPTAYSQVTEKELEKAGVRI
                     VIYANHMLRSAYPAMVKTAETILQNGRAQEAEQYCMSISEVIRLIPPDPFSR"
     gene            16810..17940
                     /gene="aepY"
                     /locus_tag="VFE94_01500"
     CDS             16810..17940
                     /gene="aepY"
                     /locus_tag="VFE94_01500"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017140653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HZX49810.1"
                     /translation="MKSKELIDILKQLDLNFVTGVPDSGLRNFLVYVEQHPEELDHVR
                     AANEGQAVAIATGYHLATRKVPIVYMQNSGLGNAVNPLTSLADKEVYSIPMVLFIAWR
                     GKPGEPDEPQHSKMGRVMLDLLRALEIPFEIAQVEAGEVYTQLVSLKEQAISGRHPVA
                     LVFPKGVVEEEKGVPPFDFAQGKEEMRREEALGLLLDKIGENPIVSTTGYTSREIFEI
                     REQKNQGHHLDFLTVGSMGHASSIALAIARKTKKPVYVVDGDGALCMHMGAAATIGSY
                     APANLFHIVIDNGAHESTGGQPTLSRHLDWQKFFEAVGYKSASLVEDRESLGMFDLQA
                     TAGPAALVVKVVQGTRKDLGRPTKTPGENKEAFMRFLEETNG"
     gene            17933..>18498
                     /locus_tag="VFE94_01505"
     CDS             17933..>18498
                     /locus_tag="VFE94_01505"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZX49811.1"
                     /translation="MDSATILKEILEKEKPRKVLLFADSAYEKSGAKEKLSPVLKSFE
                     VEVFSDIAPNPTLEQIEKGIGACKSFGPDLVVAVGGGSVLDTAKAANLLAAQEGTPVS
                     YIKKEKEIAKQGKPLVAIPTTAGTGAEATRFAVVYIDNIKYSFSHEFLLPRYAVIDAE
                     LTYSLPPNVTADSGMDALAQAIEAFWST"
BASE COUNT         4953 a         4100 c         5075 g         4370 t
ORIGIN      
        1 caacccggaa cattatggtt tgcccccacg cgggagggga aaaccagggc ggcatgctca
       61 aaaacggaag aaacaccatg gctccaaggc cggcaagcgc gagtttctga aagaatgaag
      121 aagaaaaaac agtttgtgtc ataaagattc gaaaatgccg gggaaagctt gttctatatc
      181 tcgaaagagt tgagatgatt tgcggagttc gtttaagcgt accgggtcgg tggttttccc
      241 ttttgccagc gcttctttaa gaaacgcgcg cacctcttct ttggccgcgg gccgcaattg
      301 gtacacttta aagctgtata tggccatatc ttcccatgaa ccgagtttgt cgtatgctaa
      361 agcaagagag gcatagtatt ccggcctttc gtctatggtt ttgagccgca gatacacttc
      421 tacaagctgc ccatagtttt cctgctcaag gtaaaattgc agaagttggt taagaaagtt
      481 cgtagtgtaa aaatgccggg ctatatcctc gttgtacgga ggttcgagag aacctaagtg
      541 caagctatcg cgctcgccca gccagagttc cgttaccagg cgccaggcca cgcattcgct
      601 attgagggga aagcgttctg cacagttttg cgccacctct ttggcgccct ggtaatcttt
      661 ggtaagaatg agcaggtaaa ccaagcgttc gtcgacttct tcgcgtttcg ggcttaactc
      721 ctttgccttc gccagatatg atttggcggc ctcggctagt tctgtttttt cccgggggtc
      781 ttctacgagg gaaagcagga gggtggtgta agcgcccagg gcaatccatg ttttggtgtc
      841 gtgtggatgc tgctgcgaac gttcctgcag ccctgcgagc gcttgcgagg cggcttcgtt
      901 gcgttcctgc cccgcaagcc tgttgataca taaggtggta acatccacgt actggttggc
      961 cacgtaagaa tcgaggtagg tgtcggaagg aatgttttcc aaagtctgga atgcagcctg
     1021 gcagtcgcct tgctgtgcct ggaaaatggc cgcgttaagg tttttgttca tgagaaaagg
     1081 aacgaaattt gcggcataca aaaaccatac gagcaaaact cctgccagtc caattatggc
     1141 gtatttacct actgttttgc ggagcatagg caataaggaa aaaagaatag ccgacgagca
     1201 cgaagaaaac aagataggtg ccaaacgagt taaaagaaaa aatcatggct acaagataaa
     1261 ccagcagtgc ggtttgggca gcgtggatat ccaaaagcgc tgttttatgt tgcgcgcttt
     1321 tcttggcgcg ttgcaatcct cgccaaagta atccgatgaa cgcgaaaaaa gcaaacagcc
     1381 ccaaaagccc ggcgcctatt gcaatgtcaa ggtagtaatt atggaacgtg tcccaccagc
     1441 cggttgtcct cccttcaaag cgcgaactcg cagaatcaaa atgggtgtta aagggaatgg
     1501 aagagttgta cgggccatag cccaacaggg gtttttcctg aatggcggag atcgcatacg
     1561 gccacgcgcc aaagcggggc tccgtttccc atatttgttc tagcgaaagg cgatcccaga
     1621 gtcgggaaaa caaaagggtt tgctcgacga acgcaggagg ctgcactgtg tttaccagaa
     1681 tgagcaagag gaaagccaag gcaaaaagag gaagcgccac ttttttcaga aggcgcatgg
     1741 gagcagggta gaacagcaca aaaaaaaggg cgccagccgc aactcccaaa aacactcctc
     1801 gggtttccgt aatgagaatg gcatacagga ataaaagcga gagagcgccg tatgctattt
     1861 tccaccaccc ttttgctttc agaaaccaaa caatcgcaaa aggaagaaga acagataaga
     1921 atatggcaaa gaaaatggtg tttccaaaag tggcataggg ccggctttct gaactcacaa
     1981 gactgtctgc gaaaaggcca aactgttggc ctatccctat aagcacggca gcagttcctg
     2041 tggcaaagag agtgttccat atgctattcc attgttgggg gcggagaaaa agatacagac
     2101 caagcgcaaa gaggatataa aacgcatacg taagcaggcc ccaggcgcgg taaggagttc
     2161 cccagaagct gaaatgcggg tcgagggagg taaaaagcgc aaggaaaagg actcctgcat
     2221 aagcaagaag cgccaagatg ggcgcacggg cggggtctgt gcgagaaatt accctttctg
     2281 cacctttctg caagagccaa gcaagtgcga aaaaaagaag cacggcaaca atgctgcgga
     2341 agaataatgc ctgcccccag gcgggaggag caaagtaggg gaattgggaa aaaagcggca
     2401 gtataagaat agctatgatc ccagccagaa acggccaagg aagctgcaac ctgttttgca
     2461 acgcgcgttc actgctctgc atacagcttt atgataccca agatgatggg gcttgtcgat
     2521 attgcaagag aaaggaaatt gtggtattgt ttgctatatt gatattgcag tatgagcttt
     2581 gaaggaaaaa caattttggt aacaggagga acgggttcgt tcggccaggc attcgtgaag
     2641 gcccttttgc aagaccacaa cccccgttcc gtaagggtgt tttcgaggag cgaatatacc
     2701 cagtgggaga tggcgcaaaa ttttcccgat cccagactcc gctttttaat tggagatgtt
     2761 cgggataaag accggctttc gcgcgccatg aacgacgtgg atattgtggt gcacgcagcc
     2821 gctttaaagc agattgtgct gggagaatac aaccccctgg aagttattca aaccaacgtg
     2881 atgggatcgg caaatgtggt ggacgccgcc atagacaata atgtggagaa ggtaattttg
     2941 attagttctg acaaggcagt aaatcccgta aatctatacg gcgcgaccaa actgtgcgga
     3001 gaacgccttt ttgtgcacgc aaatgtgtat gtgggcgaaa accgaagaac aaagttcagt
     3061 gttgcccggt atggcaacgt ggtgggcagc agggggagcg tggttccctt attcatgaaa
     3121 caggcaaaaa gcggaaaaat taccgtaacc gacgagcgca tgaccagatt ctggattaca
     3181 ttggaacagg gagtgcgatt tgtgtcagac tgcattaacc gcatgcaggg aggggaagta
     3241 tttgtgccca aaattcccag catgcgcatt atggatctta tccaagtact ggctccttcc
     3301 tcggaggtgc agtttatcgg cattcgccag ggcgaacgac tccacgaggt gcttttggca
     3361 gaagaagaga gccgccacag caaagagttt gattcgtatt ttcttattga accggaattt
     3421 ccgttttgga aagccgaaca cggcaacgga ggaaatccca ttgcggggaa attcgtgtac
     3481 tcgtctgaca gcaatacccg atggctttcg ggggaagaaa tccaggccat gctcaaggat
     3541 gtggtatagc catgaaatgg tgctttctgg tgaataccgc gccatttctc tcggattttt
     3601 ttttgaagct cgccagcgag attgtgcgcg ggggcgacga ttgcctcgtg gttttcacga
     3661 gtaagttcgc cgaatacgaa aaaggacatt tggttcccga gggggtgcgg gttgtttcgt
     3721 ttgttgactg gtgcctgcag tctccggacg tgaaagaagt tcctcctcgc gcgctttcat
     3781 ggaaagagtt tttttccgca ttcgatcgtt tcaaaagagc gcgcatttca tatgaagacg
     3841 ccgtctctcg tatggcgcat gactacgcgt tttttagcca gcttttccga accgaacaca
     3901 tcgacgcgat ttttttcgag ccgccaagcg gggctaacac ccagattgcc tatttgctgg
     3961 ccaaagaaaa gcagattccg tatatcagcc tgcttacctc gaaaatttac aaccacctcg
     4021 atttgctcga cgtaaagtac accgattccc gatacgccgc aactttcgcg cgtttgcgcg
     4081 gggaagatct taccccagag gagaaaaaat tcgccacaga atacatagcc tcgttcatgt
     4141 ctcaccagaa aacgctttcg tacatggggc ttcaaaaaat acggttttcg ccatggagtt
     4201 ttctttccca ctacgccaaa cgcgcaaagc acgtgggaaa gattcttttt aggtacgcca
     4261 aaatccgggg gcgggcaaga cagtacgatt ttgaagcgga agcccgttgg ttgcggagca
     4321 ttcgggcccc gtttgaaacc ctgcggcgcc aggcgcgcat caggcggcag gcctcctact
     4381 accggtttgc agacaagcga gatacgggcc gtttttttct gtttcctttg cacttgcagc
     4441 cggaactttc tacttcggta tgggctacct attacaacga ccagttaaac accatacgca
     4501 acgcagcttt cgctttgcct ttcccttata agttgtatgt cagagaacac ccctcggcaa
     4561 tcggaacaaa gccgaatgag ttttatgaag aaatccagaa aattccccag gcggtgatta
     4621 tttcgcctga agaaagcgca agtcagcttg tggccgattc acgcggagtc atagcgctta
     4681 cgagcacgat tggcatggaa gccgcgcttg ccggaaagcc cgcgtatgtt ttgggagacg
     4741 cgttttatga ataccaccct ttgtgcagga gagttgcaag ttttgaggat ctcaagcgcg
     4801 ctattgaaag agacgagcac actccgcccg acctgtcgaa tttggaggga ataaatatgc
     4861 gctttattgc ttcctatatg cgaaatacgg ttgagggcac catggttgcc gcggtcaagg
     4921 aagacgactc caacgactat cgggaaatct ataaagcgtt tcgaaaaagg attgaggaaa
     4981 taaaagctct tggttgacgg gaggcggctt tttgcgtatt gtatgtctta tgagagtgga
     5041 tctcaaagga aagctcgatg tgctggtaaa ggtaaacgcg cttcttgatc aaagagaaaa
     5101 ggcgcgtttt gccggagtgg ccgccgcggc tcttgccgcc ggactttttc aggcgctggg
     5161 agtggtgtcc attctgcctt ttatcgaact ggtaatgaat cccgacacgg ctcttaaaga
     5221 gggaatactt gctctttttt ttgcctcgtt cggttttgag agcatctttg ttttcaccat
     5281 ggttgtaggt tttgccatgc tcggcattct cgtggcgggc aacctcgttt ctgcatttgc
     5341 cgagtgggtg aaaattcgct ttgtgtggca gaaaagccac gccatttccg tggctttgct
     5401 cgaacagtat ctttctctgc cctacgtcta ttttctcaat gcacacagcg cggacttgag
     5461 caaaaacatt gtttttgagg tgcagcagtt tacgacccgc ctcttaatgc cccttttgca
     5521 gatcataaca aagggaatcc ttgtggccct tattattggg ttgctgctgt ttgtggatcc
     5581 tttgatcgcc ctgggagcgg cgttggtttt tggagtttcg tactttgtgg tgtttttctt
     5641 tcttcggggt tctttgaggc aaaggggaga gcggcgtttg cgagagaata cggggcgttt
     5701 tgtggcagcc ggggaagcct ttgccggcat taaggatatt aaggctttgg ggcgggagga
     5761 atactttctg gaacggtttt cgaagcactc tgacacgttc tcgaaacttc aggcgtggac
     5821 cggagttgct attcagttgc cgcgatatgt tatggaaatc gtggcctttg gaggcgccat
     5881 ctcgctcatc ctggtgtttt tgagtttagg gcaggggagc tccgaaatta ttccgcttat
     5941 cagttttttc gccttcgccg gttaccgtgt aatgccggct gtgcagagta tatttcaggc
     6001 agcctctgat attcagtttc atagagcggt attggacagg ttcgcagagg acatgcgggc
     6061 aagtacgccg cgacagaggg ttgcgagggc aaaagagctg ccagaagcgc ttgcctttac
     6121 agaaagcatt cagctggaag gcgtatattt cgtgtacccc tacgccaaag agccggcgat
     6181 acgaaatatc aatatgcaaa tatcaaaggg cacctcggtt ggcctggtgg ggtcgacggg
     6241 tgggggcaaa acaacactcg tggacattct tatcgggcta cttcctccaa gcaagggaag
     6301 tttcaaggta gacggcgtct ctgttacgga ggggaatgtt cgcaactggc agagaaatat
     6361 cgggtacgtg ccccagcaaa tttttctggc cgacgatacc atcgcaagga atatcgcttt
     6421 tggactgccg gacgaaaaga tagacatgga caaaatagtc accgtatgca aaatggcgaa
     6481 tctgcacgac tttattcaac gagaactccc taaagggtac catacgaaga ttggagaacg
     6541 aggcgttcgc ttatcgggcg gacagcgaca gagaatcggc attgcccggg cgctcttgca
     6601 caaccccgcg gtgctggtgc tcgacgaagc caccagttcg ctcgatgggg taaccgagaa
     6661 ggcggtgctg gaagccatag gaaatgtagc taggttcaaa acgctcgtta ttgtggcgca
     6721 tcggtttgct acggtacaga attgcgatgt gatttatttt atagagaaag gccgcatcgc
     6781 agcatcgggt agttacaatg atttgcttaa aacgtacccc cggtttcggg caatggcgca
     6841 gcagccagag ccttcataat gttatgcaag atagacgcgc aaaaacaata ggcattattc
     6901 cagcgagatt ggcttctaca aggcttttgc ataagccctt gctcgatatt gccggaaaat
     6961 cgttggttca acgatcatgg gagcaggctc ttcaggctcg gttgttggac aaggttgtgg
     7021 tagctgtaga cgacgagcgc gtattcaaag aagtaacttc gttcgggggc gaagcaatga
     7081 tgacgtctgt cgaatgtcaa acaggcaccg accgggtcgc agaagccgcg cgccattttc
     7141 ccgaagcgga agttatcgtg aatgttcaag gggatcagcc ccttgttcct ccccaagcca
     7201 ttgacgaggt agcaaaggcg atgcaggaaa atccggagat agaaataagc aacctcgtcg
     7261 ccaaagcatc aagagaagac gccgaaaatc cgagcgtcgt gaagatagtg caggatcggc
     7321 aaggttttgc actgtactgt tcgcggtctc ccattcctta cccgaggaat gaagaattct
     7381 gttcctggta caaaattatg gatgtatatg ggttcaggcc cgctgccctg caaaagatag
     7441 ctgctttgcc acccacgtct ttggaaaaag cggaatccgt ggagcagcta cggattttag
     7501 aacacggcta taaaatcaaa ctcgtggaaa ctccatgcga ctttattggg gtaaacaccc
     7561 cggaagattt agaaaaggta agagcgctct tgagccatgg ggaatcctaa tacggtaaaa
     7621 cttttggtaa cggatgtgga cggcgtgctc accgacggca agttatactt ggatgtagaa
     7681 ggcgcgcgcg agcttaagtc ttttcatgtg cgggacggca tgggtttgtt gttggccaga
     7741 gaagctggca tcaaaaccgt gtttctaagt ggcagagaca gcggagccat caggaaacgc
     7801 gcccaggaac ttcaaatcga ctacatcatt cttggatcca aaaacaagga gcaagattta
     7861 gaggaagttt taaagcaaga gggtatggcg ctgcataacg tttgtttcat tggcgacgat
     7921 gtgcaggact tgcccttatt gcgcgccgtg ggatttagcg cttctcccca tgacgcggct
     7981 aacgaggttc gtaggcaggt ggactacgtg gcggagaaaa acgggggaga aggggcggtg
     8041 cgcgacatcg tggattatat tttaaagtcc aaaatgggat tccgggaggt gacggagcgc
     8101 ctgttcgcta ttgaagaaaa aatggacctg tttaaccgga aagttgcagg cgaattcttt
     8161 tgggagttcg tgcgttcttc cgtacgaaga aaaatactgg gacaagtggg catttttagc
     8221 agaaaagaac ctcctggcaa gcgttccatg ggttactttg taggggtttt gcttcacgta
     8281 gtacaaaaca gtcttttgca taatcccttt tttgctccga aagccgacgt gctctttttt
     8341 ggcatgaaca gaaggaggct tcaggaagat ggtttatggc acgacattta ctgcgatccg
     8401 ctgatcgaag cactcgaaaa agaaggtgtt cggtgctgta tggtggaacg tcctccgttt
     8461 ggaaggccgg tgccgaggct agtgaaaacc cgtcatttgt actactttga ttttctcgag
     8521 ctttgttatc ttgtggttcg gcgcctgggc tttgggcgtg cgcgcctctc tgaagacgaa
     8581 agggcatttc ttgccaaggt gcaagagagg ttgaacgaag aattcggtac agagttgaat
     8641 cttgaaactc aactcatacg ggagttagaa aaagagaaaa tactctcttg gttatacagg
     8701 ggcttgctgc gccagatagc cccaaaggca gtgatagtag ccgacctttc tgcaaaagcg
     8761 cctttggcgg cagcgtgcaa aggtttgggc attccgctta ttgagttgca acagggagct
     8821 attcatcgct atagcctgga gtattcctat gaagggtcca agcgacaaaa gaggcgggtg
     8881 ccagattatt tttttgcttt tggagacttt tggaaaagcc atgttgccct tcctttgcct
     8941 ccagagcgcg tgttgagcgt tgggtttccg tactacgagc aggagcgaaa aaaatacatg
     9001 catggagcca gaaagaacca gattatcttt ttgtcgcagc cgacctcgag aggaacactc
     9061 gcggaatttg cagccgacct tgccagagag gttggagaca catggcacgt ggtatacaag
     9121 ctgcaccccg aagaagcgtt cgtttgggaa aaacgatatc ccgggctcgc gaattctgac
     9181 gtcgaggtgg taaaagacaa cgaaaaacct ctgtaccagc tctttggaga gtcgcgcctt
     9241 caggtgggta ctaactcaac cgctttatat gaggggctgg ggtttggttt ggaaacatat
     9301 cttttagaaa cacccgaaat ggaaatgatg taccttttag atgttggata tgctcgcaag
     9361 gtaacatctg tccaagaaat gcagagtttt ttggctaaac aggaaaattc gcagacaaac
     9421 cacggagagg agtttttccg gtcggggtcc ttggggcgca tggttactct tataaaacaa
     9481 attggtgcac tatgaagcga aaacatatcc cttacggttc ccaatggata gacgaggccg
     9541 acattaccgc agtggcaaag gtaatgcggg ggcattggtt aacccagggt cctgctattg
     9601 aacgttttga aaaaaaggtt gctgcatact gcggagccgc gtatggggta gcggttgcga
     9661 acggaacggt ggctcttcac gccgcctatg cggtagcagg gatcgggccg ggggatgaag
     9721 tgattactac gcctctgact tttgccgcta cggcaaatgc tgtagtgtat tgcgggggaa
     9781 agccggtttt tgcggatatt gaagagcata cccttaacat cgaccctcga gaagtggaga
     9841 aaaagattac cagccgaacg aaagctgtag ctccggtaga ttttgcaggg catccagccg
     9901 agtacgacga gatacgcaag atcgcaaaaa aacacaagct tctggttatt gaagacgcag
     9961 cccatgcgtt aggctccgtg taccgcggca ggaaaatcgg atctttggcc gacatgacta
    10021 tttttagctt ccatccggtg aaaactctta ctaccggaga ggggggaatg gtgctaacca
    10081 acaacaagaa ttttgcagaa aaacttaaga tgttccgaca ccatggtatt gaaaaaaagc
    10141 cggagaaagg agggtggtac tacgaaattg aaaaccttgg gtataactat cgcattacgg
    10201 atattcagtg cgcgttaggg gaaagccagt tcaaaaaact ttctgctttt cttgcccgca
    10261 ggcgtgagat tgtccggcag tataccaatg cgtttgcggg cattcctgct cttatcttgc
    10321 cccaagagaa gccgtatgtg caggcagcgt atcatatcta tgtggtgcag tttcggctgg
    10381 agcttttagg cgccgatcga aaaacgttat tcgaaaagtt gcaggagaag gggatagggg
    10441 tacaggttca ttatatgcct ttacacctcc acccctttta tcaaaagacc ttcggttata
    10501 agaaaggaga ttttccacgg gccgagcgtt actacgaaag agcgcttacc ctgccgcttt
    10561 tccccaagat gaccgacaag gaagtgaagt atgtcattcg gggggttcaa aagctggttg
    10621 aacagtacca aaaatgaaaa tctacgcaat tgtgcaggcg cgtacaacat cgactcgact
    10681 tccgggcaag gtactggaag agattgaggg aaaacccatg gtgtggcatg taatgaacag
    10741 agtgctcgcg gcagaaaccg tggataaagc tgtccttgct tttcccgaca caaaagagaa
    10801 cgatatgata cagatgctcg cagaaaagca tctgtgggaa tacgcaagag gaagcgaaga
    10861 cgacgtgttg gcacgatatg tctctgcgtt gactttgttc ccctgcgacc acgttgtgag
    10921 aattactgcc gactgcccgc ttgccgaccc ggcgatcatt gacttggttg taaaagaaca
    10981 tctcgaatct tcagcagact atacatcgaa tacgctaaag cgcgatttcc ccagagggtt
    11041 ggacgttgag gttttccgtg ttgtcgttct tgaacaagca catagggaag cgaagcagcc
    11101 ttatgccaga gagcacgtaa cgccgtatat atatgaacat ccggaggtgt ttttacttcg
    11161 ttctgtagag gctccaaaag cgttaaagcg gccggatttg cgcttaacgg tggataccaa
    11221 agaagatttg gagcttgttc gagcaatcta tgggcatttg tatgaaccag gaaagatatt
    11281 tggattgcaa gaggtcatag cactattcca tcaatatccc gacttgaaag acataaatgc
    11341 tgcggtgaag caaaagacct tgggagatta gccgtatgaa tattgttact atccaacaga
    11401 gaactattgg tagggggcag ccgtgcttta ttgtggcgga actttcaggg aaccaccacc
    11461 agaactacga agaagccgtg gagttggtaa agcttgcaaa agaagccgga gcagacgccg
    11521 taaagctgca aacctatacc ccagatacca ttacgctgga ttcgcgaaac aaatggttta
    11581 tcgtggaagg agcagaccgg ccggattcct ggcagaaaaa gagcctgtac gagctttacc
    11641 agcaagccta taccccatgg gaatggcagc caaaactcaa agctcttgca gatgagctgg
    11701 ggattttgct gttctccagc cctttcgacg aaacggcagt agattttttg gaacaggaag
    11761 tagagccccc ttgttacaaa gtggcttcgt acgaagcagt gcatattcct ttacttaaaa
    11821 agattgccgc cactggcaaa ccgataattc tttctattgg ctttgcggat ttggaagaag
    11881 ttacccttgc tttgcagaca ctgcgggaaa acggcgccac agaaattgcg gtattgcatt
    11941 gtgtaacctc gtacgcggag agcccgcgga tagaagatat gaacctgcgt actatagctg
    12001 atattacaga acggtttggc gtgccaagcg gcttttcaga caacaatggc ggcgtcgaaa
    12061 ttccggtggc ggctgcggtt ttgggcggag cttctattat tgagaagcac tttattttag
    12121 accatgggac cggaagcccg gatgcaaggt tttccattga tcctgaagaa ttcaagcgta
    12181 tggtgcagtt gattcgcaga gcagaggcag gcgaaagaga agaagtgctc aaagaaattg
    12241 gcgttgagaa tgcagaagcc gccatgggca acgtgcacta cggccccgca aaccagcaag
    12301 aagaagataa caagcagttt cgcccgggca tttgggcaaa agccgacatt gcaaaaggag
    12361 aggtattcac caaagaaaac attcgcgtgg cccggcccaa tgcacctgat agctttatgc
    12421 ccaaggattt tgactggatt ctcggcagga aggcgcaagc tgatattgcg tttgccaacc
    12481 ccatcacccc agaggttgta caacgcagcc gagtaagaag agcaacgcct caagatgcag
    12541 aggcaatatt ggaaatccgc aacagcaaag aagccagaat gcagtctgca gaaaaccagg
    12601 aagaaattcc tttggaaaag catatccagt ggtttcaaaa acaatacttc aaagaggggg
    12661 caaaaaatgc ctgctatgtg gcggagcttg aaaaccgcgt ggcagggtac tgcaggttcg
    12721 acgaaaaaga agacgaagtc tttgaagttt ctatagccat agcctccggc ttccagggga
    12781 agggtttggg atctttgctg cttttagaga gtttagaaca gttcccggat tcaaaaaaag
    12841 tcgttgcaaa agtaaaaaag gacaacaaag cttccaatcg gctgtttgaa aaatgcggct
    12901 ttcgagttgc caaagaagat cctttgtttt ttacgtatgc gtattctcgt taacgccgcg
    12961 ggtactattg tacgatcgag tgtgtccttg caggaaggag tcaatgtgct gggcagggcg
    13021 tatgggcgtg ttgctgaaca ctttgttctt cagcctctcg gccaaggcga cctggaacag
    13081 gaagtttcac ctgcaagtct cgacgcttcg ggagtaattc tgttcgtgaa gcaggggaga
    13141 gtcaggtggg ccgctccaga cgtctcggcg agcagagact tgttttatgc caaaaaggaa
    13201 agcggcgtgt gggagctttc cgacaatttt tttgatattg cttcgagttt ttctcaagta
    13261 agctggcgca agcaaaacct catgttcttt ctgcggcatg ggtacttttc tcccggactt
    13321 actttttttg aagaaatttc ccgcgtgcgc gtcggctgca aactcgtctt tggaaaggaa
    13381 ggggtgcaag aggaaagcgt ttgggaatca catggttcag aagatcgaag ctacgaggtt
    13441 tttaaaaagg cgctgaattc tgtgttggag gtgcgggggc tagaagacgc ccatgctctg
    13501 cttttgagcg gagggtgtga ctccggttta ttggcggcca ttggcacaaa agtattgggc
    13561 aagcatccgc ttagctttac gagtgtgtat gcacagaccc tgaaagtaaa tgaacaggac
    13621 ggcttcaaag cagagaaggt gagctcgcac ctaggcttag agcatgggac cctccatctc
    13681 gatttggcca aagagacggt acaacctttg tatttgctgg tggagcatat gcctttggcg
    13741 gcccactttg gcatagcgtt tttccggata tacgaagaac tcgccagacg cgggcggacc
    13801 aaggtatgga cgggccagaa cgcagacagc gtgtacaact ttggtcctac cgggagaagc
    13861 aaaggaggca tgttaaaacg ctggtatctt tctaaagaat actgggagtc gcttcccgac
    13921 attactactc gaaagccgtg gaaccaagtc atgaaatggg ggggagaagc agggatggtg
    13981 gccttgcgca cccaaaaggg aatgcgcgtt cgccagccca aaacatttgc cgaactcctc
    14041 tccgtgtttg aaaacgaaga aggatcgttc cggtttccca aagccggcag cccgcgccag
    14101 gagcatatgg gcattggccc tctttcgtat ggggaagcga agcatttgtt ttttgaccgc
    14161 aaaatacaga cgtttcttac gggcggagat ccccgggtgc actacggagc agcagacgga
    14221 actggcagcg aggccatgct cgcgtattct gcggccaaca tgatggcctt ttggcggggc
    14281 ttgcccctgc agctgcaaga cgtgttctct ccgaaacgat atatttatag gtacctgcga
    14341 gagttgctgg gaaagaaagc ctacaaaaat ctgtacgaaa cgcctgttcc cctttcctcg
    14401 gcgaaaccat acataacctg ggcagaatgg caggagcagg tattggagag tacgcgcttt
    14461 ggcaaagagc tgcgggaagc tattggaggg ctgtctgaat ttccctttct tgccgaaatc
    14521 ttcaacccgc atgtgttgca ccatcccctg gacgcctttt ggctgctaga agttcttgaa
    14581 aaagtgaaaa aacaaggcgt tcacgtgaga gagatgatat tgtaaaagat ttctgttact
    14641 atacatatac ccatgttgcg gttattggaa aagttttgcc aaagggcagc caattttgta
    14701 gggtacaaaa ttgttaaaat agaaaaaagt gcggatcctt ttcgggatcc tgattttgag
    14761 cgcctgtacg aaaaatgcaa gaagcacact atgacttcca aagggaaagt ctatgctctg
    14821 tataacgccg taaagtatat tctgcaggcc gggattccgg gcgactttgt agagtgcggg
    14881 gtgtggagag gaggcaatac tatgctcatt gcctacatgc tgcttgaaat gcaagacacc
    14941 agccgaaaga tttacctcta cgatactttc gaagggatgg tagaacccac cccaaacgat
    15001 tatacctttc taaaccagat tcctgcggta ctgcgttgga aacgcgagca aaaagatgcc
    15061 tacaacgagt ggtgttttgc tcctttggaa gaagtgcagg aagccatgtt ttctacgggc
    15121 tatcccaaag accgcatggt ttttgtgaaa ggcaaggtgg agaaaaccat acctcaaacc
    15181 atgccagagc agataagctt gcttcgtctg gacacggact ggtacgaatc ttccaggcat
    15241 gagctggcgc atttgttccc tttgctggcg cgaaaagggg tgctcatatt agacgattat
    15301 cgctattggg caggccagaa aaaggcgacc gatgagtatt ttacagaaac caaaactcct
    15361 atgttgctcg tggacaacat tggaggagcc accgtgggcg tgaagataag ctagcacgct
    15421 ccttgcacat tttgtcaaat gcgctataac agagtattac atagggcatg tttttaatat
    15481 gaaaaagcaa gcaaaagcca aaaaagaacc cgtggtatat gtaggcatgg cagccgactt
    15541 aattcaccac ggccatgtca atattatcga cgaggcaagc aagctgggcg aggtcgtggt
    15601 aggcctctta acaggcgagg caattgccag ctacaagaga gtaccctttt taaaattcga
    15661 agagcgaaag tttgtgatcg aaagaatcaa gggggtaaaa gaggttattg cccaggaaac
    15721 gctggactat gtgccaaacc tgaaaaaggt aaaaccggat ttcctggtgc atggggacga
    15781 ctggaggacc ggcgttcaaa gagaaacaag agcgcgggct attcgagcgc tcaaggaatg
    15841 gggaggcaaa ttggtggagc ctacgtatac ctcgggcatt tcttccaccg atttaattaa
    15901 gtatgcaatt gaagctggca ttactcctac gcaaagactg caaaggatgc gccggcaact
    15961 ggcggtaaag cccttgttgc ggtttatgga ggtgcacaac gggttaagcg ggcttattgt
    16021 agaaaacacc acagcactcc gcgacaaacg aaaagtggaa ttcgacggca tgtggggaag
    16081 cggacttacc gactctaccg ccaaaggcaa acccgacaac tccgtggtag atattacctc
    16141 gcgcctgcaa agcttggagc agattttgga cgttacgact aaacccatat tggtggacgc
    16201 tgacaacgga ggtttgtccg agcattttgt gcataccgtg agaaaagccg aaggcctggg
    16261 catttcaggg ctggtggtgg aagataaaat tggcgccaag cgaaattcgc tgtttggcac
    16321 cgatgttgcc caagagcaag acaccataga agattttgcg gcaaaaatta cagcaggaaa
    16381 gcgctcgcag gtaaccgacg actttatgat tatcgccaga atcgaaagtt taatattgaa
    16441 aaagggccag aaagacgcgt tggccagagc acacgccttt attgaagccg gggccgacgg
    16501 cattgttatg cacagcaaag aaaaaactcc ggcagaaatt cttgcttttt gcaaagagta
    16561 caaaaagttt accaataggg tacctttggt ggttatccct actgcttaca gccaggtaac
    16621 agaaaaagag ctggaaaaag cgggggtgcg cattgtgatt tacgcaaacc atatgctgcg
    16681 ctcggcttat cctgccatgg taaagacagc cgaaaccatt ttgcagaacg ggcgtgcgca
    16741 ggaagccgaa caatactgca tgtccataag cgaggtaatc cgtcttattc cgccagatcc
    16801 ttttagccga tgaaaagcaa agaactcatc gatattttaa aacaactcga cttgaacttt
    16861 gtaaccggcg tgccagatag cggcttgcgc aattttttgg tgtacgtaga gcagcatcca
    16921 gaagagttgg accatgtgcg tgctgccaac gaggggcaag cagtggccat tgccaccgga
    16981 taccacttgg ctacgcgcaa ggtgcccatt gtgtatatgc aaaattcagg gctgggaaat
    17041 gcggtaaatc ccttaacttc gctggccgac aaagaagtat atagcatccc catggttttg
    17101 tttatcgcct ggcggggaaa gcccggcgaa ccagatgaac cccagcactc caaaatgggc
    17161 agggtaatgt tggacttgct gcgcgctttg gaaattcctt ttgaaattgc gcaagttgag
    17221 gctggagaag tgtacacgca actggtctcg ttaaaggagc aggcgatttc tggccgccac
    17281 cccgtagctt tggtgttccc taaaggcgtg gtagaggaag aaaaaggcgt accacccttc
    17341 gacttcgctc agggtaaaga agaaatgcgt agagaagagg cgttggggct tttgctcgac
    17401 aaaatcggag agaatccgat tgtttcgact acgggctaca catcgcgcga gatttttgaa
    17461 attagagaac agaaaaacca gggccatcat ttggatttcc taacggtggg gtctatgggc
    17521 cacgcttctt cgattgcgct tgccattgca agaaagacaa aaaagcccgt atacgtggtg
    17581 gacggcgacg gagccctatg catgcacatg ggagctgcgg ccaccattgg ctcttacgct
    17641 cctgcaaact tgtttcatat tgttattgac aacggcgccc acgagagtac aggcgggcag
    17701 cccacgcttt cgcggcattt ggactggcaa aagttttttg aagcagtagg gtataagtct
    17761 gcttcgcttg tggaagatcg ggagagcctc ggaatgtttg atttgcaggc aacggcaggg
    17821 ccggcagccc ttgtagtaaa agtggtgcaa ggtacgagaa aagatttggg caggccaaca
    17881 aaaactcctg gcgaaaacaa agaggcgttt atgcgttttt tggaagaaac caatggatag
    17941 cgcaacaata ctcaaggaaa tcctcgaaaa agaaaagcca cgcaaggtgc ttttatttgc
    18001 agacagcgcc tacgaaaaaa gcggggcaaa agagaaactt tcgccagtgc tcaaaagctt
    18061 cgaggtcgag gttttttctg acattgcgcc aaaccccacg ctggaacaaa tcgagaaggg
    18121 gataggggcg tgcaaaagtt tcgggccaga cttggttgtc gccgtgggcg gcggaagcgt
    18181 gttggatact gcaaaagctg caaatcttct tgcagcgcaa gaaggaactc ctgtttccta
    18241 tataaaaaaa gaaaaagaaa ttgcaaaaca gggaaagcct cttgtggcca ttcccacgac
    18301 agccggcacc ggggccgaag ccacgcgttt tgccgtggtg tatatagata acatcaaata
    18361 ttctttttcg cacgaatttt tgcttccgag gtacgctgtt atagacgccg agcttacgta
    18421 ttctttgccg ccaaatgtta cggcggattc cggtatggac gctttggctc aggccataga
    18481 ggctttttgg agcaccaa
//
LOCUS       DASRUP010000035         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_502184, whole genome shotgun sequence.
ACCESSION   DASRUP010000035 DASRUP010000000
VERSION     DASRUP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502184"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFE94_01510"
     CDS             complement(<1..472)
                     /locus_tag="VFE94_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49812.1"
                     /translation="MAEKSKAQRSKRKHSSIIKSNIQKHMSLKRILSFVLLPVFALGL
                     YTFPSAASANGGDVTITNNNSAYVFNHVKVEAETGDNQANGGDGGDAGDGGDGAEGGE
                     GGEAGDGGMGGDAGMGGDGGDGGDGGYGDGDGDGGDGEDGGDAGMGGDGGDGGAG"
     gene            1056..2291
                     /locus_tag="VFE94_01515"
     CDS             1056..2291
                     /locus_tag="VFE94_01515"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX49813.1"
                     /translation="MEQLRTLAFQRKMPLPKFAIFVPARSEGDVIANTIKNLEEIAYP
                     KSRYTAFIVTDERELAEQVEARTKDVAWATTQELNLKHGQNFLRCVEIPKWYSGKSTK
                     GRALNYALQHLVASPEWGTFDMIGVLDADGRFHSNVLREAAHLRLYKKAKLLQGPVYQ
                     ITNFAQVSLVGIAAGLELAIHHLTELPDRLRPGNRLQFLAGTNYFIDKELISAVGGWN
                     QHALVEDAELALRLYIEKRICGHWLNVVEKEQTPPSFKVYRKQRERWARGHLRLLGYI
                     QKSNIPFWQKIDLFYRIFSTQYRSFFDVGLLLLALCLLLSGALQQAGLFFTVLSFFFL
                     AATPLLWDLYGFMYRQIAPSLEPAAKRKQQFLMSAKLFLFMPVLIFIQAIPRVEALYN
                     HFIRRQQVEWYKTARTKEV"
     gene            2301..3455
                     /gene="wecB"
                     /locus_tag="VFE94_01520"
     CDS             2301..3455
                     /gene="wecB"
                     /locus_tag="VFE94_01520"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZX49814.1"
                     /translation="MKQFTCCIVLGTRPEIIKMAPVIQACQEQEVSFFLVHTGQHYSY
                     RMDKIFLEELGLPPAKYHLEIGSGSHGYQTGGMLVRIEQILFDERPDVTLVQGDTNTV
                     LAGALACAKSSTRIGHVEAGLRSYDRTMPEEINRMLTDHVADFLFAPTDTSKQNLLRE
                     GVAPENIFVTGNTIVESIRNHVEMAQQKSSILAKLKLRQQNYFLLTAHRAENVDYRER
                     LEGLLEGFRQIGEFYDIPLVWPIHPRTKKNIHIFGLEKPFRAIPRLRVVEPLGFWDFL
                     RLMSNAKLVLTDSGGMQEETCILKIPCVTLRENTERPETLEVRSNILAGTDPERMFAA
                     AQEMLGRQREWNHPFGDGTTGNQIIEILKRFSGDTEKVEGIHLEMNSVKI"
     gene            3478..>4377
                     /locus_tag="VFE94_01525"
     CDS             3478..>4377
                     /locus_tag="VFE94_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49815.1"
                     /translation="MQKPIKIFFVSLFFFAFVASQTGLAFAEVTNLAAGKPYTSAPAP
                     NDPYGDDGTKLTNGQLAQLTPTDQGWVGYGFDEPVISFTLDSPAALSQIRAHFLEETG
                     WGIHYPQAVEVQTSLDNQAWTSQGNLVQQGEYFELNLAQIEANHVRLLITRMFWTFIS
                     EIEIYGEPIAPTPQPLKNVLFLTSTPDNNNESYNRMINTLDSMGFSYDVISDEQITST
                     NLHNYQLVVVDGTSFELLDINLAEEQQIVASIEQDVNYLWIGAGIWGSFQTTNLPNAF
                     GLQYIAEGTSPEFGVTEAEFTDLT"
BASE COUNT         1173 a         1141 c          996 g         1067 t
ORIGIN      
        1 cacctgcacc tccatcgcca ccgtcaccac ccatgccagc gtcgccgccg tcttcaccat
       61 cgccgccatc gccatcgccg tcgccgtaac caccatcgcc gccatcgcca ccgtcaccac
      121 ccatgccagc gtcgccgccc ataccgccat ccccagcttc accgccttcg ccaccttctg
      181 cgccgtcgcc gccgtcgcca gcgtcgccac catcaccgcc atttgcttgg ttgtcgccgg
      241 tttcagcttc tacttttacg tggttgaaca cgtaagcaga gttgttgttt gtaattgtta
      301 catctccgcc atttgcagaa gctgcagagg gaaatgtgta aagtcccagt gcgaacactg
      361 gaagaagtac gaaacttaag attcttttca atgacatatg tttttgaatg ttagacttta
      421 ttatgcttga atgtttacgt ttcgaccttt gagctttgga cttttctgcc atacaggcag
      481 aaaaaaaatc caagccccgt gcttttttcg cgtgaaaaag cgccgggcct ggatttcttg
      541 atggtatctg atggtgatgg gaaaacagta aaacgcatgt agtgtgcacc cttttctcgt
      601 tttccccgaa gagaagggcg tccgaacatc atgtcctagg cgaaagacat tattaaagga
      661 tactcggacc atgacatagt gtcaagccca ggaggaagag cggagttttc cacaatttgc
      721 aatttgctaa agtgcgctac aataacgtaa tggcaatgcc gaaaaataaa ggcgcaggga
      781 cggacatagg cgctgaagtc agggaaaacg gtcaggtatc cactttgatg gtggacacct
      841 gacgtgattc agcgcccatg tacgaccctg cttcctatca tagtgttaat ctatttgagc
      901 aatgaatgtc aataggcacg tcctatcata ccccaatgtt tgattatacc ctctcgtttc
      961 tgtacatagc gtttggtatc ggtgtatttt tgtatttttt aaatgtgtac cgaatacgca
     1021 agtacataaa agaactcaac accccgcttg tctcaatgga gcaactacgg actctggcgt
     1081 ttcaacgcaa aatgcctttg cccaaatttg ctatttttgt tcccgccaga tcagaaggcg
     1141 atgttatagc aaacactata aaaaatcttg aagaaattgc ttacccaaag agcaggtaca
     1201 cagcgtttat cgttaccgac gaacgagaac tcgcggaaca ggtggaggcc cgtaccaaag
     1261 atgtagcctg ggcgacgaca caggaactta atctgaaaca cggccagaac tttttacgat
     1321 gcgttgaaat acccaaatgg tacagcggaa aatccaccaa ggggagggcg ctcaactacg
     1381 ccttgcagca ccttgtggca agccccgaat ggggcacttt cgatatgatt ggcgtactgg
     1441 atgccgacgg caggttccac tcaaacgtgc ttcgcgaagc ggcgcatctg cggctttaca
     1501 agaaagcaaa acttcttcag gggccggtgt atcaaattac caactttgca caggtatcgc
     1561 tggtgggtat cgctgcgggt cttgaacttg ctattcacca tttaaccgaa cttcccgatc
     1621 ggctgcgccc ggggaataga cttcagtttt tggcaggaac taattacttt atcgacaaag
     1681 aactcatctc tgcagttggc ggctggaacc agcacgctct ggtggaagac gcggaactcg
     1741 cgctccgctt gtatatagaa aagaggattt gcggccattg gttgaacgtg gtggaaaaag
     1801 aacaaactcc tccttcgttt aaagtgtacc gaaagcagcg cgaacgctgg gcaagaggcc
     1861 atttgcgctt gctgggctac attcaaaaat caaacatccc cttttggcaa aaaatcgact
     1921 tattttacag gattttcagc acgcagtacc gttcgttttt cgacgtcggg ctgcttctgc
     1981 ttgcgctttg cctcctgctt tccggcgcct tgcagcaggc aggtcttttc ttcaccgtac
     2041 tatctttctt cttccttgcc gccacccccc tgctgtggga tttgtacgga ttcatgtacc
     2101 gccaaatagc cccttctctt gagcccgccg caaaaagaaa acagcaattc cttatgtcgg
     2161 ccaaactttt tcttttcatg ccggttctta tctttattca agccatcccg cgcgtagaag
     2221 ctttatataa tcactttatc cgccgccaac aagtagagtg gtacaaaaca gcccgaacga
     2281 aagaagttta acgcaatcgt atgaaacaat ttacctgctg catcgtatta ggaacaagac
     2341 ccgagattat caaaatggcg ccggtgattc aggcatgcca ggagcaagaa gtttcatttt
     2401 tcctggtgca caccggacag cactactctt atagaatgga caagattttt ttggaggaac
     2461 tcggccttcc ccctgctaaa tatcacttgg aaattggttc tggctcccat ggatatcaaa
     2521 caggaggcat gctggtgcgc attgaacaaa ttttgttcga cgaacgcccc gacgtaacat
     2581 tggtgcaggg cgacacaaac accgtgctgg caggagccct ggcttgcgca aaatcttcga
     2641 cgcgcattgg gcacgtggag gctgggctgc ggagctacga ccgcactatg ccagaagaaa
     2701 taaaccgcat gctcacagac catgttgcag actttctgtt cgcgcccacg gatacttcca
     2761 aacaaaacct gctacgggaa ggagtggcgc cagaaaacat ttttgtcaca ggaaatacta
     2821 tcgtagaatc aataagaaat catgtggaaa tggctcagca aaaatcctcg atccttgcaa
     2881 aattgaaact gcgacaacaa aactactttt tactgaccgc acatcgggca gaaaacgtag
     2941 attatagaga gcgcctggaa gggctgcttg aaggtttccg ccagattggc gaattctacg
     3001 atataccctt ggtgtggccc attcacccaa ggacaaaaaa gaatatccat atatttgggc
     3061 tcgaaaaacc attccgcgct atcccccgcc ttcgcgttgt agaacctttg ggcttttggg
     3121 actttttacg tcttatgagc aacgccaagc tggtccttac ggattctggc ggcatgcagg
     3181 aagaaacctg tattttaaaa atcccctgtg ttacgctaag agaaaacacg gaacgccccg
     3241 agactttgga ggtacggtca aacatactgg caggaactga tcctgaaaga atgttcgcag
     3301 cggcccagga aatgcttggc cgccaaagag agtggaacca tccctttggc gacggcacca
     3361 ccggaaacca gattatagaa attttaaaac gcttttctgg cgacacagaa aaggtcgaag
     3421 ggatacacct tgaaatgaac tccgtaaaaa tctaaatcgg tattaggcaa taactctatg
     3481 caaaaaccca taaaaatctt tttcgtttcc ttgttctttt tcgcgtttgt ggcaagccaa
     3541 accggtctgg cgtttgccga agtaaccaac cttgctgccg gaaaacctta tacatcggcg
     3601 ccagccccca acgatccata tggcgacgac ggaaccaaac ttaccaatgg gcagctggcc
     3661 cagctcactc ctaccgacca aggctgggta ggatatgggt tcgacgaacc tgttatttcc
     3721 tttacgcttg attcgccggc cgcgctttcc caaatacgcg cgcatttttt ggaagaaacc
     3781 gggtggggca ttcattatcc ccaggcagta gaagtgcaaa cctctcttga taaccaggca
     3841 tggacctctc aaggcaatct tgtccagcaa ggcgaatact ttgagctcaa cctcgcacaa
     3901 atcgaggcaa accacgtacg gctgctcatt acccgcatgt tctggacgtt tataagcgaa
     3961 attgaaattt atggagaacc catagctccc acaccccaac ccctcaaaaa cgtgctgttc
     4021 ttaacgagca cgcccgacaa caacaacgaa agctacaacc gcatgataaa tacgttggac
     4081 agtatgggtt tttcgtatga tgttatttca gacgaacaaa ttaccagcac caacctgcac
     4141 aactaccagc tcgtcgtagt ggacggaaca tcgtttgagc ttttggacat taaccttgca
     4201 gaagaacagc aaattgtcgc ctctattgag caggatgtaa actacttgtg gatcggggca
     4261 ggcatttggg gatcgttcca aaccaccaac cttcccaacg cctttggctt gcaatacata
     4321 gcagaaggca ccagcccgga gtttggcgta acagaagccg aatttaccga ccttacc
//
LOCUS       DASRUP010000036         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_397195, whole genome shotgun sequence.
ACCESSION   DASRUP010000036 DASRUP010000000
VERSION     DASRUP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397195"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFE94_01530"
     CDS             complement(<1..198)
                     /locus_tag="VFE94_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49816.1"
                     /translation="MKKIFVFAALAFAFAPPLSASAQGITLNLDYPVFGGIDLNTQQN
                     INAIVGWLYYGIVGIAGLAAFV"
     gene            complement(215..715)
                     /locus_tag="VFE94_01535"
     CDS             complement(215..715)
                     /locus_tag="VFE94_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49817.1"
                     /translation="MAVGDATTMQHLSEQDALHLRQQEEAAQRSYEAALVSPLDADFL
                     LVLCLAMMTDALDIVLEIFAFLIFPKALGIVIDILALGIVFWIKWRTDQVIDRKTHRE
                     TMKSQYSEKLRGAAGRRGSAMASARTPLRRFWLRMSFVFLLELIPFVGIIPFWTITVL
                     GVLRQR"
     gene            complement(718..>2460)
                     /locus_tag="VFE94_01540"
     CDS             complement(718..>2460)
                     /locus_tag="VFE94_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HZX49818.1"
                     /translation="DNIKLGNWTAKSFFIFSYPRYLSTAWLSSAINLDMPMDISLFLH
                     PIDTGYILRRLRRRVTEVQSEIMEEESKGLVRNPELETAFQDMEGLRDRLQTAQERMF
                     RFGLYITVYAQSVEELREIQTTLRSIFESRLVYIKPALYQQKEGFQSCLPYGTDLLQV
                     HNSMNTEPLSSMFPFVSFDLSSNEGIMYGINRHNNSLILFDRFSLENANSTIFATSGS
                     GKSYAVKLEILRYLMWGIDVIVLDPENEYKTLAEAAGGSFFSISLSSDHHINPFDLPQ
                     PREDERAEDVLRSNVINLVGLMRIALGGLTPEEDAIMDRAITETYAAKDITPNSNPLT
                     WKERVPLMSDLESVLETMEGTDSLVRRIRKYTKGTYANFFNQPSNISLGTNIVSFGLR
                     EMEDSLRPLAIYIIMRHIWNTVRSELKKRIFVLDEAWWIMQSEDGASFLFGMVKRARK
                     YWLGVTTITQDVEDFLRSSYGKPIITNSSLQLLMKQSPASIDVVQQAFNLTSEEKNLL
                     LETDIGEGIFFAGQKHVAIKVVASASEHELITTAPEELARRQAREEKPGAETPKFGQE
                     RIEPNDPYKQDNTV"
BASE COUNT          603 a          597 c          635 g          625 t
ORIGIN      
        1 gacgaacgca gcaaggcctg caattcccac aatcccataa tacagccatc ctacgatggc
       61 gttgatgttc tgctgcgtgt tcaaatcaat gcctccgaat acggggtagt cgaggttgag
      121 ggtgatgccc tgggcggagg cggagagagg cggggcaaaa gcaaatgcca gtgcggcaaa
      181 cacaaaaatt tttttcatta gtatatgtta cttattagcg ttggcgcaat acccctaaaa
      241 ccgtaatcgt ccaaaaagga ataatgccca cgaagggaat gagttcgagt aaaaacacga
      301 aagacatgcg cagccaaaaa cggcgaagag gggttcttgc gctggccatg gccgatcccc
      361 tgcgccctgc agcgccgcgc agtttttcgg aatactggct tttcatggtt tcccggtgcg
      421 ttttgcggtc gataacctgg tcggttcgcc atttaatcca aaacacaatg cccaaagcga
      481 gtatgtcaat aacaatcccg agggcctttg ggaaaattaa aaatgcaaaa atctccagta
      541 caatgtcgag agcgtcggtc atcatggcga gacacaagac caaaagaaaa tctgcatcaa
      601 gcgggcttac cagagccgct tcgtaactac gctgcgccgc ctcttcttgc tggcgcaagt
      661 ggagcgcgtc ctgctcggag aggtgctgca ttgttgttgc gtcgccgact gccatactta
      721 caccgtgtta tcttgtttgt aggggtcgtt tggttctatg cgctcttgac caaattttgg
      781 ggtttctgcg cccggttttt cttctctggc ctggcgtcgc gcgagttctt cgggagccgt
      841 ggttatcagt tcgtgctccg aggcagaagc caccacctta atggcaacgt gtttttgccc
      901 ggcaaaaaat atgccttctc ctatgtcggt ttccaaaagc agattttttt cttccgaagt
      961 taagttaaac gcctgctgca ccacgtcgat acttgctggc gactgcttca ttaacagctg
     1021 caaagaagag ttggtgataa tgggttttcc gtaggaagag cgcaaaaaat cttccacatc
     1081 ctgggtaatg gtggttacgc cgagccagta ttttcttgcg cgctttacca tgccaaagag
     1141 aaaagaagcc ccgtcttcag actgcatgat ccaccacgct tcgtcgagca cgaaaatgcg
     1201 cttttttaat tccgagcgca ccgtattcca gatgtggcgc atgataatat aaattgccag
     1261 ggggcgcagg ctgtcttcca tttcccgcag cccaaaggaa acaatgtttg tgcccaggga
     1321 aatgttgctt ggctggttga aaaagttggc gtaggtgccc ttggtgtatt tgcgaatacg
     1381 ccttaccaaa gagtcggttc cctccatggt ttccagcacc gactccaagt cagacatcaa
     1441 aggaacgcgc tctttccacg ttagggggtt ggagtttggc gtaatgtctt tggcggcata
     1501 ggtttcggta atcgcccggt ccataatggc gtcttcttcg ggggttaggc cgcccagcgc
     1561 aatacgcatt aaacccacaa ggttaataac gttggaacgc aatacatcct ctgcgcgttc
     1621 gtcttctctg ggttgtggaa gatcgaaagg gttaatgtga tggtcagaac tcaaagagat
     1681 gctaaaaaag cttccgcccg cggcttccgc cagtgttttg tattcgtttt cggggtcgag
     1741 cacgattacg tcaatccccc acattaagta ccgcaaaatc tccaacttca cggcgtaact
     1801 ttttccggat cccgacgttg cgaaaatagt ggaattggca ttttccaaac taaagcgatc
     1861 gaacaaaata agagaattgt tgtggcggtt aatgccgtac atgattcctt cgttggagct
     1921 taagtcaaac gacacaaacg ggaacatcga agacaagggt tctgtgttca tggagttgtg
     1981 cacctgcaat aaatcggttc cgtagggcag gcacgactgg aacccttctt tttgctggta
     2041 gagcgcgggt ttgatgtaca ccaagcgcga ttcaaaaatg gagcggagcg tggtttggat
     2101 ctcgcgcagt tcctctacgc tttgcgcata cacggtaatg tacaggccaa accggaacat
     2161 gcgttcctgg gccgtttgca agcggtcgcg cagtccctcc atatcctgga acgcggtttc
     2221 cagttccgga tttcgcacca ggcctttcga ttcttcttcc atgatttcag actgcacctc
     2281 ggttactctt ctgcgaaggc ggcgcaaaat ataccccgta tctatggggt gcaaaaacag
     2341 cgaaatgtcc atgggcatgt cgaggttaat ggcagaagaa agccaggcgg tgcttaagta
     2401 tcttgggtaa gaaaagataa aaaaagattt tgccgtccag ttgccgagtt ttatgttatc
//
LOCUS       DASRUP010000037        14125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2085427, whole genome shotgun sequence.
ACCESSION   DASRUP010000037 DASRUP010000000
VERSION     DASRUP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14125
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085427"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1047
                     /locus_tag="VFE94_01545"
     CDS             187..1047
                     /locus_tag="VFE94_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZX49819.1"
                     /translation="MITKFIIFINLFLISFLCFEVATAAEFRVESDAASLGVGEEFLV
                     TLVVDTSGEAINAFEGKVVFQESLLELQGVREKGSVVSLWVQDPPSFSGLTPGGYSGR
                     GVLFSLVFKAKAEGEGSVAIEQGRALLNDGKGTEAPLEIINYQFSIINQSSNSQILMT
                     ETKDTEPPELFELKIGQDPSMFDGKPFLVFATQDKNSGIDHYEISFLYKGKTTDWQLA
                     ENPFLLEHEEDLEKIWVKAVDRAGNERIATLDVRNLFFRFLLLGNAGILGGIGAVFVF
                     WLWRRKSSFS"
     gene            1020..3056
                     /locus_tag="VFE94_01550"
     CDS             1020..3056
                     /locus_tag="VFE94_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZX49820.1"
                     /translation="MAKKIFLFVICFFSFFIWGEAEAATLSVQPSTGTFQVGSTFDVS
                     VYIDTQKETINAIEAYLHFPADKLQLVSPATGSSIIDLWISQPKYNNAAGTIELRGGI
                     PQGVNVSKGLVSTLMFRVRDTGSALVSFADNSRVLLNDGQGTDKLQQTYSGVFSLVLP
                     PPAGPVVVSETHPVQTEWYADNDLAFTWSQDEGVDGYSYVLSQEPIAIPDGISEGTEN
                     GVRFRDVADGRHYFHLKALRSGIWGGATHFAARVDTAPPAEFPIEIFPSQKTTERRPT
                     IQFQTTDAASGLNRYEVKIVPLKQENASGKEESQPFFFEVESPYIASALELGTYDVIV
                     RTYDNAGNFRDVPERLSIVTPLFEVVGQDGVHFRNMGTVPWPVLWTVIGILAVLFALL
                     GLVFRKKFKALHAGQERKQFPDALQKQLEELKKYQRKYGKLAIVLVFGVSLLLAGCVK
                     PGFTHAQQQEELSPPVISQFARNISNDEIFYAGGVTEFPRTRVVIFLQNEQSGELTSR
                     SINSDERGEWFYQHDTFLAPGSYFVWAQGERGEEKSAPSSHREFSVTTGAFEFAGARV
                     SYEVIALLVSIVLLLALLGTGLFMFTHMRAARRKHKEFQKEMREAQESIRRGFAVLKR
                     DIQSELAIVKRAGLGKGLSAEQKKQEDQLLRDLAWAETYIGKEVWDVADTEHEH"
     gene            3117..3824
                     /locus_tag="VFE94_01555"
     CDS             3117..3824
                     /locus_tag="VFE94_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HZX49821.1"
                     /translation="MSNELLFLILSLLGPLLIVFTLYFGRESLFVVIAVNLILVSALG
                     SEIISLVGAVTNAGNVFYALAIFALAILHELYGKKTAQRAVWVGFIGVVIFVVMTQVT
                     IRFSESAFSQVSQGSFEIVFEQIPRIAFASSVAYLIAAHFFVWFYDRLRRKLTGFQFI
                     GIAYLVGAFAMQIVDSILFFSVAFGGLMPASILLQIMVVGFLSKFAVAIVSMPFLYWS
                     RRFLGKGQINKAYEQDR"
     gene            3849..4862
                     /locus_tag="VFE94_01560"
     CDS             3849..4862
                     /locus_tag="VFE94_01560"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZX49822.1"
                     /translation="MTIDTSIYDIAKHMAAKGKGILAADESTGTADKRFAALGVSQTE
                     EMRRQWRQLLLTTPGIEEFLNGVILFDETIRQQTDQGVGFAKLLQGKGVIPGIKVDQG
                     TVDYDGHPGEKITQGLDGLAGRLQEYYAMGTRFTKWRAVITIGESIPSQECIQKNAEI
                     LAEYARVSQEQGFVPIIEPEVLLEGDHTIEKAEEVTTNTLKTVFVEVQKAGVDLQSLI
                     LKSSMVLAGKDCAEQANSQQIAEATIRTFQNSVPKDVAGIVFLSGGQSPQQATENLNE
                     IIKLGPQQWPLTFSFSRALQEPVLDLWRGNPANVPTAQQAFLKRLRLNYTALSGDYTP
                     DME"
     gene            4944..5372
                     /locus_tag="VFE94_01565"
     CDS             4944..5372
                     /locus_tag="VFE94_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZX49823.1"
                     /translation="MEEKILKELRGQGHRITAVRREMVKMLVNAKAPLAASDILKVLQ
                     KEKLKANKTTVYRELEFLVEQNIAQEVEFGDKKKRYEISDKHHHHVVCVECKNVEDVD
                     LQADLDGVEKKIAKQKGYKIINHSLEFFGLCAKCLPRSTG"
     gene            5378..6115
                     /locus_tag="VFE94_01570"
     CDS             5378..6115
                     /locus_tag="VFE94_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZX49824.1"
                     /translation="MNTFLSILFATGFISLVSFSGALFLFFQKHLVQRISLFLLAFAA
                     GSFMGAAFLHLLPEAVEQVESANSFLFILLGFGLFFFIENIFHWHHVRNDEPEHKSLG
                     MLTLLGDGVHNFLDGMIIAAVFFVDIKLGFAATLAVALHEIPQEIAEFGVLIHAGYSR
                     MRALLFNFLSASTVIAGGILSFFLEAWLGQWIALFMLFAAGVFIYVAASDFVPEMRKE
                     RSLSKSLALLATFAAGVLLMWLVLFLE"
     gene            6304..8694
                     /gene="ppsA"
                     /locus_tag="VFE94_01575"
     CDS             6304..8694
                     /gene="ppsA"
                     /locus_tag="VFE94_01575"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HZX49825.1"
                     /translation="MRRTLKASRAKATILWFEEVDKRDARLVGGKNASLGEMYSNLQH
                     LGITVPNGFAITTNAYKAFLERNGLGKEIKNLLKKLDAADLKNLQETGKAIRQLILDA
                     ELPRDLQRRILQAYARLGRRASVAVRSSATGEDMAEASFAGQQETYLNVVGEQELLQA
                     VKKTFASLFTDRAISYRKDKGFNSFGALSVGVQKMVRSDKGCAGVLFTIDPETGFNKV
                     VVINGAYGLGEMVAQGEVTPDEFLVFKPMLEQKKGIISKSLGPKKQKLVYSDAAGGGT
                     KKVAVPERDRNKFCLEDKEILKLAIWGVQIEQHFKKPMDIEWAKDGVSGKLCIVQARP
                     ETVHSALQGAYREYALKTANVPLVSGMGVGMKIAAGKVHVIATPRDMGKFQKGEILVT
                     EITDPDWEPIMKSAAAVITDKGGRTSHAAIVSREFGIPAIVGTGNATKKLKTGQMVTV
                     DCSSGNEGRVYQGALKFEVKERQLERIPETKTKIMLNIGAPDEAFKNAHLPVKGVGLG
                     RLEFIINSYIKIHPNALLDYKKLPSKTKKQIDQFTQGYTDKKQFYVDKLAEGIAKIAS
                     AFWPNEVIIRFSDFKTNEYRQLIGGELYEPVEQNPMLGWRGASRYYDPRFQKAFGLEC
                     QAIKKVREEMGLQNVTPMVPFCRTSQEGKKVVALMEQSGLSRELDKGLRIYVMCEIPS
                     NVILADEFLDVFDGMSIGSNDLTQLMLGLDRDSGMGAKIGNENDSAVKQMIGAAIAKC
                     VERGKYVGICGQAPSDYPEFARFLVEQGIESMSLNPDVVIPTIAKVAEFEKYIKQT"
     gene            8696..9712
                     /locus_tag="VFE94_01580"
     CDS             8696..9712
                     /locus_tag="VFE94_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007545542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HZX49826.1"
                     /translation="MNIAFFRIEPWEREYIESREEFKKLGAKAVFFDEGLDQDHIPTD
                     TGAEVICTFIDSKITKPVLDAFPNVKLITTRSTGFDHIDLEECKKRGIQVANVPKYGE
                     NTVAEYTFGLILALVRKIYHSYDQIRETGSFDLQGMRGMDLKGKTLGVIGTGSIGQNV
                     ARIARGFEMQVIACDKYPNQQAAKEVGFDYCEPERAIRESDILTLHVPYTKETHHLIN
                     KDNIFTMKKGAYLINTSRGQVIETEALVKALHEGHLGGAALDVFEEEGVIKDELEFLL
                     QGSSEKPDLKTVLANHVLVDMPNVIVTPHNAFNTWEALQRILDTTIQNMRGFQEGKPV
                     NLVG"
     gene            9721..10665
                     /gene="mltG"
                     /locus_tag="VFE94_01585"
     CDS             9721..10665
                     /gene="mltG"
                     /locus_tag="VFE94_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZX49827.1"
                     /translation="MVLKGILVAVGLLVATCTVFVWQAVVLPKDSGSAEQYSFAVTAG
                     ENAFQIAKHLEQAGLIRSRIGFDVYAVVRGIRRDLKAGTYFLSPSMRVEEIAEKIVAG
                     EDSPLTIRILEGWRAKDIARYLQDRGIVEGEDFLLASQNLEGYLFPDTYQIASDTSAQ
                     AIVEMMQENFEGKLTAEFYAAVQRQGKTLNDIVIMASMLEKEVQSKEDKKVVAGILWK
                     RMAQGIPLQVDATVAYGSTEEDELSQVELQTPSPYNTYLIQGLPAGPISNPGAESLEA
                     ALFPVETAYLYYLSALNGTTIFSETLEEHNIQRVKHLR"
     gene            10713..>14125
                     /locus_tag="VFE94_01590"
     CDS             10713..>14125
                     /locus_tag="VFE94_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HZX49828.1"
                     /translation="MDAKQSLESPAPPEGGSFEQQKNVPAKRKRIFLFTLLALIGLGV
                     AGFLFWQWQFGGGEKPAYTQTYRLVPEKVSQSAAIPVSLPPGTDRETARENISFEPAI
                     EGEWVDQPQQKTSWIPFLKTANAAGDTLSDEPAVLLFKSKEDLKLNRYYAVQLANFEG
                     GVIKADFLAVENPAIVAVFPKEQSEAPEDSEITIVFSRPMVPLTTLGELEEKDIPVEI
                     TPATQGKFKWITTRNLQFIPAERLVRSSRYTVKIKEGFVSMDGLPLAGGEYQFTSRQL
                     RYVRGSGGEQMFNRPISLHFNQPIDLGRTLSEISVRDNKTGQTVPFVAEFEKPGGGDG
                     LFSSSLDIGLQRFFAQVRGTFGLTLFGNPQVGSTDGNGAVLWIYKQSDRFGRKKLWDF
                     EGSYTVRVNKVYPAEGDIILDKSIQASVSIPTIIRQITAESERTSDVNQDFFDPQGKI
                     WISFYEDIDLGRSDLEIPKSKEIGYGEKCADSAIQFSSNVQCEKVPDKSRVFVLFESS
                     QVGLGEQIQIRFDKVLNTEGLELNKEPFTRTITAYPAFQVLGSFPGNGDSNTSLVEFT
                     FCTNTPLNPPTKEELEQYIKANLPFELQAWARSWKVTRVFSQTKCSMGEFETRITYGL
                     MPQEQYRLEFSLSDVFGQRQDYSLAIATGPMPSSQLGLYHLQAGYNVTPPSKTKLTYA
                     AKNMEFVNLEICKLSPRNFLYYLENEPAGYESSGAAASCEQVVQNTISLPKRFWLKNY
                     FTVDIRDYMPDPIGHYVVTLTHPAYQKNFWINGGNVLRTAYERTFVSVTNLAAAEKRI
                     QPQVANFGSGGKPLSQKELQALQNIYWVSNTSTLDPVPGAQVQLYQGRNLALAGSATT
                     NEQGVAFAAVVSNISGAVITSGADSTVIPSSESKVDYASIASSAERIYLYTDKPLYQP
                     GQEIFIKGIDRVGYDGNYEILEGETVSVQVFNSKQDEIFNRKLLLNGFGTFDTKVFLE
                     ANAPLGSYRICAQEYACISVDVQQYVPAAFEVTLQPDKEEYISKDTVKLNVDANYYFG
                     VPVEAGEVTYTVSSQNYYFDRFSDEYFSFGTQSYYEYPFSYGDKFLLRGTTSLTAGKA
                     EIQQLLDLGELLGDEAGRGKLIIFDVTLQTADGKSVSAQKSFVAHPGEL"
BASE COUNT         3736 a         3063 c         3784 g         3542 t
ORIGIN      
        1 agatgctggg agatttgagt ggagacggcc gcacggacct catagacttt tcgatattga
       61 ttttctggtt cgaacacccg aaccctccaa gccatgttga cttgaatgga gacggcaagg
      121 ccgacctcgt ggactttagc atcatggcgt attattggac gggctagtaa gatttcaaca
      181 gttataatga ttacaaagtt tataattttt ataaatcttt tcttgattag tttcttgtgt
      241 ttcgaagtgg cgaccgcggc ggagtttcgg gtggaatcag atgcagcaag cttgggcgtg
      301 ggcgaagaat ttttggttac tcttgtggtt gatacctcgg gcgaggcaat aaatgctttc
      361 gaaggcaaag tggtgttcca ggagtcttta ctcgagctac agggtgttcg cgaaaaaggt
      421 tcggtggtga gtttgtgggt gcaggatccg ccttcttttt ccggcttaac tcctgggggg
      481 tacagtggaa ggggagtact gttttctctt gtatttaagg caaaagcaga gggagaggga
      541 tctgtcgcaa tcgagcaagg gcgagcgtta ctcaacgatg gaaaaggaac ggaagctccg
      601 ctggaaatta tcaattatca attttcaatt atcaatcaaa gctcaaattc ccaaatccta
      661 atgacggaaa cgaaagatac ggaacctccg gaactctttg agctgaaaat cgggcaagac
      721 cccagcatgt ttgatggcaa gccgtttctg gtttttgcta cacaagataa aaactccggc
      781 atagaccact acgaaatctc ttttctttac aagggtaaga ccaccgactg gcagctcgca
      841 gagaatccct ttttgctcga acatgaggaa gatttggaaa aaatctgggt aaaagcggta
      901 gaccgggccg gaaacgagcg aattgccacg ctcgacgtaa gaaacctctt ttttcgcttc
      961 cttctgcttg gaaatgctgg tatacttggt ggaatagggg cagtgtttgt tttttggcta
     1021 tggcgaagaa aatcttcctt ttcgtaattt gttttttcag tttcttcatt tggggcgaag
     1081 ctgaagcggc cacactttcc gtgcagcctt ccacgggcac gtttcaggtg gggagcacgt
     1141 ttgacgtttc cgtatatata gatacgcaaa aggaaacaat aaacgcgatt gaagcctacc
     1201 tgcattttcc cgcggacaaa ctgcagcttg tttcgcccgc aaccggaagc tccataattg
     1261 atttgtggat tagccagcca aagtacaata acgccgcggg aaccattgag cttcggggcg
     1321 gaattcccca gggcgtaaat gtttcaaaag gcttggtttc cacgcttatg ttccgcgtgc
     1381 gcgatacggg atcggcgctt gtaagttttg ccgacaattc ccgggtactg ttaaacgacg
     1441 ggcagggtac agataagttg cagcaaacgt atagcggagt attcagcctg gtgctgcctc
     1501 cccccgccgg gcctgttgtt gtttcagaaa cgcatccggt acaaacagag tggtatgccg
     1561 acaatgacct cgcgtttact tggtcacaag atgagggagt ggacggttac agttatgtgt
     1621 taagccaaga acccattgcc attccagacg gtatttcaga aggtaccgaa aacggcgtgc
     1681 gctttcgcga tgttgccgat gggagacatt acttccactt aaaggcgctg cgtagcggca
     1741 tttggggcgg agcaacgcac tttgcggcgc gtgtggatac tgcgccgcct gccgaatttc
     1801 ccatagaaat tttcccttcc caaaaaacaa cagaacgccg gcctactatt caatttcaga
     1861 caaccgatgc cgcttccggc ttgaatagat acgaagtaaa aattgttccc ttaaaacaag
     1921 agaacgcgag cggtaaagaa gaaagccagc cgttcttttt tgaagtggaa agtccttata
     1981 tcgcgtcggc tttggagttg ggcacatacg acgttattgt gcgcacatac gacaacgccg
     2041 gtaactttcg cgatgttcca gaacggcttt ccattgtaac acccctgttt gaagttgtag
     2101 ggcaggacgg cgtccatttt cggaatatgg gcaccgttcc ctggcctgtt ttatggacgg
     2161 taattgggat tttggctgtg ctttttgcgt tgcttgggct ggtgtttagg aaaaaattta
     2221 aggcgttaca cgcggggcaa gaaagaaaac agtttccgga cgcgctgcaa aaacaactag
     2281 aggagctgaa aaaataccaa agaaaatacg gcaagctggc tattgtactt gtgtttggag
     2341 tttccctttt gctcgcgggt tgtgtgaaac caggtttcac acacgctcaa cagcaggaag
     2401 aattgtcgcc gccggttatt agccagtttg cgcgcaacat ttcaaacgat gaaatttttt
     2461 acgcgggagg ggtaactgaa ttccctagga caagagtggt tattttcttg caaaatgagc
     2521 agtcgggcga gcttaccagc cgttccataa attcagacga acgaggcgag tggttttatc
     2581 agcacgatac gtttcttgct ccaggctctt actttgtatg ggcgcaaggc gagcgcggcg
     2641 aggaaaagag cgcgcccagt tcgcatcggg agttttccgt aactacgggc gcctttgagt
     2701 ttgccggcgc aagagtaagc tacgaggtaa tcgcgctgct ggtaagcatt gtgttattgc
     2761 tggcgctgtt gggaaccggg ctgtttatgt ttacacacat gcgcgcggcc cgccgaaaac
     2821 acaaggaatt ccaaaaagaa atgcgagagg cccaagagtc tatccgccgc gggtttgccg
     2881 tactcaagcg cgatattcag tcagaattgg caattgtaaa aagggcgggc ctgggaaaag
     2941 gattatctgc agaacagaag aaacaggaag atcaactgct tcgcgatttg gcctgggcag
     3001 aaacctatat tggaaaagaa gtatgggatg ttgcagacac ggaacatgaa cactaatatc
     3061 ctatgttctt caaacgaact ttggaatttt aaagaaagat ttggtatgat acctatatga
     3121 gtaacgaact cctttttctt atactttctc ttctcggccc attacttatt gtttttactc
     3181 tctattttgg gcgggaaagt ttatttgtag tgatagcggt aaatcttatt ttggtgagtg
     3241 ctttgggttc ggaaataatt tcgctcgtcg gcgccgtgac gaatgcagga aatgtctttt
     3301 acgccctagc gatttttgcg cttgctattc tccacgaatt gtacggcaaa aaaacggccc
     3361 aacgggcggt ttgggtgggg tttattggag tagtgatttt tgtggtgatg acgcaagtta
     3421 ccattcggtt ttcggaatcg gcatttagcc aagtatccca aggatccttt gagattgtat
     3481 tcgaacaaat accccgaatc gcctttgcga gctcggttgc gtatttaata gccgcccatt
     3541 tttttgtatg gttttacgat cgtctgcgca gaaaactcac gggttttcag tttattggga
     3601 tagcatatct tgtgggcgct tttgccatgc aaatagtcga cagcatactg tttttctcgg
     3661 ttgcgttcgg cgggttgatg cctgcgagca tattgttgca aattatggta gtcggatttc
     3721 tctccaagtt tgccgtagca atagtgagca tgccgttttt gtactggagt cgcaggtttc
     3781 ttgggaaggg ccaaataaat aaggcctatg aacaggatag ataaaataac ccaaagcttt
     3841 gtaaggatat gaccatagac acttcaattt acgacattgc aaagcacatg gcggcaaagg
     3901 gcaagggtat tttggctgcc gatgaaagca caggtacggc agacaaacgg tttgccgcct
     3961 taggcgtgtc gcaaacagaa gaaatgcgcc ggcagtggcg gcagttgctt ttaactacgc
     4021 ccggcatcga ggaatttcta aacggcgtta ttttatttga cgaaaccata cgccaacaaa
     4081 cagatcaggg cgttggcttt gctaaactat tgcagggcaa aggcgtaatt cccggcatta
     4141 aagtagatca gggcacggtg gattacgacg gccatcccgg cgaaaaaatc acacaagggt
     4201 tagatggttt ggcaggcagg ctacaagaat actacgcaat gggaactcgt tttaccaaat
     4261 ggcgcgcggt cattacaatt ggtgagagca ttccttcgca agagtgcata cagaaaaacg
     4321 ccgaaatttt ggcagaatac gccagggttt cgcaggaaca gggttttgtg cctattatag
     4381 agcctgaagt gcttttggag ggtgaccata ccatagaaaa ggcagaagaa gttaccacca
     4441 atacgctcaa aaccgtgttc gtggaagttc aaaaagcggg cgttgatttg cagagtttga
     4501 tattaaaatc cagcatggtg cttgctggca aagattgcgc agagcaagca aacagccagc
     4561 aaatagcaga ggccactatt cggacatttc aaaattccgt gccaaaagac gtggccggca
     4621 ttgtctttct ttccggcggc caaagtccgc agcaggcaac agaaaatctc aacgaaatta
     4681 ttaagctcgg cccgcagcaa tggcctttaa cgttttcctt ttcacgcgcc ctgcaagaac
     4741 ccgtgctcga tctctggcgc ggcaacccag caaacgtgcc aaccgcccag caggcctttc
     4801 ttaagcgcct gcgtttgaac tataccgccc tgtcagggga ctacacacca gatatggaat
     4861 agaagaaacc gcccgttcgg gcggcttttt gattgggggg gtgtctcttg acaaacgcaa
     4921 ctgggttgcg ttagagtata tacatggagg aaaaaatact aaaagagctt agagggcagg
     4981 ggcatcgtat tacggcggtg cggagagaaa tggttaaaat gctggtaaac gcgaaggctc
     5041 ccctggccgc ttcagatatt ctcaaggttt tgcaaaaaga gaaacttaaa gcaaataaga
     5101 ctacggtgta ccgcgagctg gagtttttgg tggagcaaaa tattgcccaa gaggtggagt
     5161 ttggcgacaa gaaaaagcgg tacgaaattt cagacaagca ccatcaccac gtggtgtgcg
     5221 tggaatgtaa aaatgtggaa gatgttgatt tgcaagctga tttggatggc gtggagaaaa
     5281 aaatagcaaa acaaaaaggt tacaaaataa taaaccattc actggaattt tttggcctgt
     5341 gcgccaaatg cctgccccgt tctacggggt aagtaatatg aacacatttt tatctatact
     5401 atttgctacc ggttttataa gtttagtgtc gttttccggg gctttgttcc tgtttttcca
     5461 aaagcatttg gttcagcgca tttccttgtt tttgctggcg tttgccgcgg gatcctttat
     5521 gggagccgcg ttcttgcatt tgctgcccga agccgtggaa caagtagaat ccgcaaactc
     5581 gtttttgttt attttgcttg ggtttggctt gtttttcttt atagaaaaca tttttcactg
     5641 gcaccatgtc agaaacgacg agccagagca caagtcgctt ggcatgctta cgctgttggg
     5701 tgacggcgta cataactttt tggacggcat gattatagct gccgtatttt ttgtggatat
     5761 taaacttggc tttgctgcta ccttagctgt ggcgttacat gaaattccgc aagaaatcgc
     5821 ggagtttggc gtattaatac acgcaggata ctccagaatg cgcgccttgt tgtttaactt
     5881 tttgtcggcc agtaccgtaa ttgcgggagg catactaagt tttttcctgg aggcgtggct
     5941 gggccaatgg atagctttgt ttatgctgtt tgccgccggc gtgtttattt atgtggcagc
     6001 ctccgacttt gtgccggaaa tgcgcaaaga acgaagttta tccaagtcgc tcgcgctttt
     6061 ggccaccttt gccgccggcg ttctcttaat gtggctggtg ctgtttttag aataaatctt
     6121 ttccctcgcc cgcagataag aattgaatat catttgttcc attttattga cgatcgtcac
     6181 tttcgtggaa caggtggaat taaggtggtc gcttgaaatg ccgatcggcg tttcaagcga
     6241 cctatggggc gaatgcggtg ggtgttgttt ttggagtagt attcgtggta gagtaggaga
     6301 attatgcgga ggactcttaa ggcaagcagg gcaaaagcca ccatactttg gtttgaggag
     6361 gttgataagc gggatgccag gttggtgggg gggaagaacg cctcgttggg cgaaatgtat
     6421 tccaatctgc agcatttggg cattaccgtg cccaatggtt ttgctattac cacaaacgcg
     6481 tataaagctt ttttggagcg caacgggctg ggaaaagaaa ttaagaatct tctcaaaaaa
     6541 cttgatgctg cagacctcaa aaacttacag gaaaccggaa aggcaatccg ccagcttatt
     6601 ttggatgcag agttgccgcg cgacctgcag cggagaattt tgcaggcata tgcaaggctg
     6661 ggaaggaggg caagcgttgc cgtgcgttct tccgcaaccg gcgaagacat ggcagaggcc
     6721 tcttttgccg gccagcagga aacgtactta aatgtagttg gagaacaaga gttactgcaa
     6781 gcggtaaaaa aaacgtttgc ctctttgttt acggacaggg caatttccta ccgcaaagac
     6841 aaagggttta atagttttgg ggcgctttct gtgggcgtgc aaaaaatggt gcgctccgac
     6901 aaggggtgcg cgggagtttt gtttaccata gaccctgaaa ctggctttaa caaagtagtg
     6961 gtgataaacg gtgcttatgg gttgggagaa atggtggcgc aaggcgaagt aacgccagac
     7021 gagtttttgg tgttcaagcc catgctggaa caaaagaagg gaattatatc aaaaagcctt
     7081 gggcccaaaa aacaaaagct cgtatactct gacgccgccg gtgggggaac aaaaaaggtt
     7141 gcagtgccag agcgcgaccg caacaagttt tgcctggaag acaaggaaat tttaaaactc
     7201 gccatttggg gcgtgcagat cgagcagcat tttaagaaac ccatggacat tgaatgggca
     7261 aaagacgggg tgtctggcaa attgtgtatt gtgcaggcgc gtcccgaaac cgtgcattct
     7321 gctttgcagg gggcgtaccg ggaatatgcg cttaaaacag caaacgtgcc tttggtaagc
     7381 ggcatgggtg ttggcatgaa gatagccgcg ggaaaagtgc atgtaattgc aactccgagg
     7441 gacatgggca aattccagaa aggagaaatt ctggttaccg agattaccga tcccgattgg
     7501 gaacctatta tgaaatcggc cgcagcggta attaccgaca agggagggcg taccagccat
     7561 gccgccattg tttcaagaga gtttgggatt ccggccattg tgggtacggg aaacgccacc
     7621 aaaaagctca aaacagggca aatggtaacg gtggattgtt cttctggtaa tgaaggcagg
     7681 gtgtatcaag gcgctttgaa atttgaagta aaggaacgac agctcgagcg catcccagag
     7741 acaaaaacca agataatgct taacattggc gctcccgacg aagcgtttaa aaacgcgcat
     7801 ttgccggtaa agggagtagg gttgggcagg cttgagttta ttattaattc gtatattaaa
     7861 attcacccca acgcccttct ggattataag aagctgccct cgaaaacaaa aaagcaaatc
     7921 gaccagttta cccaaggtta caccgacaaa aaacagtttt atgtagacaa gctggcggaa
     7981 ggcattgcga aaattgcctc ggccttttgg cccaacgagg ttattatccg gttctccgat
     8041 tttaaaacca acgagtatcg ccagctcata ggtggggaat tgtatgaacc tgtcgaacaa
     8101 aatcctatgc tcggctggcg aggcgcctcc aggtactacg acccgcggtt tcaaaaagct
     8161 tttggtttgg agtgccaggc cataaaaaaa gtgcgggaag aaatgggttt gcaaaatgta
     8221 acgcccatgg tgcctttttg ccgaacctct caagagggca aaaaggtagt ggcgttaatg
     8281 gagcagagcg ggctttcgcg ggagctcgat aagggccttc gtatatatgt aatgtgcgaa
     8341 atcccttcga acgtaatctt ggccgacgaa tttttggacg tgttcgacgg gatgtctatc
     8401 gggtctaacg acttaacgca gctgatgctg gggttggacc gggactctgg catgggagca
     8461 aaaattggta acgaaaacga ttctgcggta aaacaaatga taggggcggc aatagccaaa
     8521 tgcgtagagc gtggcaagta tgtgggcatt tgcggccagg cgccgtctga ctacccggaa
     8581 ttcgccaggt tcctggtgga acagggcatt gaaagcatgt cgttaaatcc cgatgtagta
     8641 atccctacta ttgccaaagt cgcagaattt gaaaaatata taaaacaaac atagtatgaa
     8701 catagcattt tttcgaatag agccgtggga gagggaatat atagagagcc gcgaggagtt
     8761 caaaaaactt ggcgccaaag ccgtgttttt cgacgagggg ttagaccaag accatatccc
     8821 gacagacaca ggggcagaag ttatctgcac gtttattgat tccaagatta ccaagccagt
     8881 gctcgacgca ttcccgaatg tgaaacttat taccacgcgc tctacggggt ttgaccatat
     8941 cgacttggaa gaatgcaaaa agcgcggcat tcaggtggca aacgtgccaa agtacggcga
     9001 aaacacagtg gcagaataca cctttggcct tattctggct ttggtgcgca agatttatca
     9061 cagctacgac caaattcgcg aaacaggaag ttttgacttg cagggcatgc gcggcatgga
     9121 cttaaaaggc aaaacactcg gcgtgattgg tacgggcagc attggccaga acgtggcgcg
     9181 cattgcacgg gggtttgaaa tgcaggtgat cgcctgcgac aaatatccca accagcaggc
     9241 ggccaaagag gtgggttttg actattgcga gccagaacgc gctattcgag agagcgatat
     9301 tcttaccctg catgtgcctt acacgaaaga aacccaccat cttattaata aagacaatat
     9361 ctttaccatg aaaaagggcg cctacttaat aaacacaagc cgcgggcaag tgatagaaac
     9421 ggaagccttg gtaaaagccc ttcacgaagg gcatttgggc ggagctgcgc tcgacgtgtt
     9481 tgaggaagaa ggcgtcataa aagacgagtt ggagtttttg ctccagggaa gttcagaaaa
     9541 gcccgattta aaaaccgtgc ttgccaacca tgtgcttgtg gatatgccca atgtaatcgt
     9601 tactccccac aacgccttta atacgtggga agcgctgcag cgcattttag acacaacaat
     9661 ccaaaacatg cgaggcttcc aggaaggcaa gcccgtaaat cttgtggggt aaaataacat
     9721 atggttctga aaggaattct tgttgcggtg ggcttgctgg ttgcaacctg cacggttttt
     9781 gtatggcagg cggtggtgct ccccaaggat tcgggtagcg ccgagcaata ctcttttgcc
     9841 gtaaccgcgg gagaaaatgc tttccaaatt gcaaagcatt tggaacaagc gggcctgata
     9901 cgcagtcgga tcggctttga tgtgtatgcg gtggttcgag ggattcggcg cgatctgaaa
     9961 gcaggtacct attttttgag tccctctatg cgtgtagagg agatagcaga aaagattgtg
    10021 gctggcgaag attcaccact tactatacga attctcgaag gctggcgtgc gaaagacatt
    10081 gcccgctatt tgcaagatcg aggtattgtg gaaggcgaag actttttact cgcaagccaa
    10141 aacctcgaag ggtacctttt tcccgacacc tatcaaattg caagcgatac gtcagcccaa
    10201 gcaatcgtgg aaatgatgca ggaaaacttt gagggaaagc tcactgcaga attctatgcc
    10261 gcagtccaac gtcagggcaa aaccctcaac gacattgtta ttatggcctc tatgttagag
    10321 aaagaagtgc aaagtaaaga agataaaaaa gtagttgccg gaattttgtg gaagcgcatg
    10381 gcgcagggta ttcccctgca ggttgatgca accgtggcct atggctctac cgaagaagat
    10441 gagctttctc aagttgaact gcagactcct tctccctata atacgtacct tatccaaggg
    10501 ctgcctgctg ggccgatctc aaacccgggc gccgaaagtt tggaggccgc attgttccct
    10561 gtggagactg cgtatttgta ttatttgtct gcccttaacg gcactacgat attcagcgaa
    10621 acgctcgagg agcataatat tcaaagggta aaacatttaa ggtagttgct tgtggatagc
    10681 gaaaatatac gggtaggtta taatacggta atatggacgc caagcagagt ttagaaagtc
    10741 ctgcgcctcc cgaaggcgga agtttcgagc agcaaaaaaa tgtgccggcg aaaaggaaaa
    10801 gaatttttct ttttaccctg ctggccctga ttggtcttgg ggtggcaggt tttctttttt
    10861 ggcaatggca gtttggagga ggggaaaaac ctgcgtatac ccaaacctat cggctcgtgc
    10921 cagaaaaagt ttcgcaatct gcggcaattc ccgtcagtct gcctccggga actgacaggg
    10981 aaacggcgag agagaatatt tctttcgagc ctgccatcga aggcgaatgg gtggaccagc
    11041 cgcagcaaaa aacgagctgg atcccatttc taaaaacagc caacgccgcg ggagatacac
    11101 tttctgatga acccgcggtt ttgctattca agtccaaaga agatttgaag ttgaaccgct
    11161 actacgccgt gcagcttgcg aactttgagg gaggtgtgat taaggccgac tttcttgccg
    11221 tggaaaatcc ggcgattgtc gccgtattcc ccaaggaaca atcggaggcg cctgaagatt
    11281 cggaaatcac catagtgttt agccggccta tggtgccttt aacaacgctt ggggaactcg
    11341 aggagaaaga tattcccgtc gagattacac ccgccaccca aggaaaattt aaatggatta
    11401 ccacgcggaa cctgcaattt attcctgcag aacggctggt tcgttcttcc cgctacacgg
    11461 tgaaaattaa agaggggttt gtctcgatgg acggcttgcc gcttgcgggg ggcgaatacc
    11521 agtttaccag ccggcagttg cgctatgtga gggggtcagg cggtgaacag atgtttaacc
    11581 ggccgatctc tttgcatttc aatcagccca ttgatttagg gcgtacgttg tctgaaatct
    11641 cggtacgcga caataagact ggccaaacgg ttccgtttgt ggcggagttt gagaaaccgg
    11701 gcggcgggga cggcttgttt tcttcttcgc tggatatcgg gctgcagagg ttttttgctc
    11761 aagtaagggg aacgttcggg cttactctct ttggaaatcc ccaagttggt tccaccgatg
    11821 gcaacggggc ggttctctgg atttataagc aaagcgaccg tttcgggcgg aaaaaacttt
    11881 gggacttcga aggcagctat acggtacggg tgaataaggt atatcctgcg gagggagata
    11941 ttatcctcga taaaagcatc caggcaagcg tatctatccc taccattatt cgccagataa
    12001 ccgcggaatc cgaacgaacc agcgacgtca accaagactt ctttgaccct caagggaaaa
    12061 tatggatctc gttttatgaa gatatcgatt tgggaagaag tgacctggaa attcccaaat
    12121 cgaaagaaat agggtatggc gagaagtgcg cggattccgc tatccagttt tcgagcaatg
    12181 tgcaatgtga gaaagtgccc gacaagtcgc gcgtgtttgt tctattcgag agcagccagg
    12241 ttggtttggg tgagcagatc caaatacgtt ttgacaaagt cctaaacacc gaaggccttg
    12301 agctcaacaa agagccattc acccgaacca ttactgctta tccggcgttt caggttctcg
    12361 gaagtttccc cggcaatgga gattccaaca cctcgcttgt cgaatttacc ttttgtacca
    12421 acacgccctt gaatcctccg acaaaggaag aactggagca gtatataaag gcaaacttac
    12481 cttttgaact gcaggcctgg gcaaggtcgt ggaaggtaac gcgcgtattt tctcaaacga
    12541 agtgcagtat gggggagttt gagaccagga ttacctatgg cttgatgcct caagagcagt
    12601 atcgtctgga gttttcttta agtgatgttt ttggccagcg gcaagattat tcgttggcaa
    12661 ttgccaccgg gcccatgcca agttcgcagc ttgggctgta tcatttgcag gcagggtata
    12721 acgttacccc tccctcaaag acaaaactca cctacgctgc caaaaacatg gagtttgtga
    12781 acctggaaat ttgcaaactt tctccgcgca acttcctcta ttatctggaa aacgagcccg
    12841 cagggtatga gtcctcgggc gccgccgcca gctgcgaaca agttgttcag aacaccattt
    12901 ctttgccgaa gcgtttttgg ctcaagaatt attttacggt agatatccgg gattatatgc
    12961 cagatcccat tggccactat gtggtaacgc ttacccatcc tgcataccaa aagaattttt
    13021 ggatcaatgg cggaaacgtg ttgcgcacgg cctatgaacg gacgtttgtg agcgtaacca
    13081 acctggcggc ggcagaaaag cgcatccaac cgcaggtggc aaattttgga tcgggcggaa
    13141 agcctttgag ccaaaaggag ttgcaagcgt tgcaaaatat ctactgggtg agcaatacct
    13201 ctacactcga tcccgtcccc ggtgcccagg ttcagttgta ccagggcagg aaccttgcct
    13261 tggctggcag tgctaccacc aatgagcagg gtgttgcttt tgcggccgtc gtctcgaaca
    13321 tttcgggagc ggttattaca tcgggagccg atagcacggt catcccaagc tcggaaagta
    13381 aggtggatta cgcaagcatc gcctcctctg ctgaaaggat ttacctgtat accgacaagc
    13441 ctttgtacca gcccgggcag gaaattttta tcaaggggat tgaccgcgtg ggatacgacg
    13501 ggaactacga aatcctcgaa ggagaaacgg tatcggtgca ggtgttcaac tccaagcagg
    13561 acgagatctt taacaggaaa ctgctcttga acggctttgg gacattcgat accaaagtgt
    13621 ttctcgaggc caatgccccg ctcggctcct ataggatttg cgcgcaagag tacgcgtgca
    13681 tttcagttga tgtgcagcaa tatgtgccgg ctgcgtttga agttacgttg caacccgaca
    13741 aggaagaata tatttccaaa gacacggtga agcttaacgt ggacgccaac tattactttg
    13801 gggtgcccgt agaggcaggc gaagtaacct atacggtaag cagccagaat tactatttcg
    13861 accgcttttc agacgagtac ttttcattcg ggacgcagag ctattatgag tatccttttt
    13921 cctacggcga taagtttctg ctgcggggta ccacaagcct gacagccggg aaagccgaaa
    13981 ttcaacagct cctcgatttg ggagaattgt tgggagatga agctggccgg gggaaattga
    14041 ttatttttga cgttaccttg cagacagccg acggcaagag tgtttccgcg caaaagtcgt
    14101 ttgtggcgca tcccggcgaa ctgta
//
LOCUS       DASRUP010000038         5588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_991269, whole genome shotgun sequence.
ACCESSION   DASRUP010000038 DASRUP010000000
VERSION     DASRUP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991269"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2367)
                     /locus_tag="VFE94_01595"
     CDS             complement(<1..2367)
                     /locus_tag="VFE94_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZX49829.1"
                     /translation="MASSFSFNAAQSTLRAALKFENYFRTSTLRKLRKITQWVFLAAF
                     LAFLLTFIPGGGIVSPTVSRLLLAAALLTFSYGTTILLLERFGEYLKAPKVPLVRHNL
                     ANAVSFEVAKAAHLAATYAKRKNFMQVDSTLLLYFLLKENASIEFIFARALLNFNEFK
                     KDVQRLLSSQVRHPGKVKETVYAPDFEESITKSIAYAQEKGRQRAEIEDLFAALANTN
                     QFFNRFLIEHNFLPERDMARIADWHYRLRVEAATRKKFWLKNSLRRYGTMGKDWAAGY
                     TITLDQFSYDISDEVRTLRFPRAVGHIQEKEMMARILSRQQMNNVLLVGNPGTGRKRL
                     IQDFASKSVLGEHESPLLNYQRVVELELTHLLAGLQSLEEAEAALNQIFKEVGAAGNT
                     ILVIDEFHNFTGEGLEQAAGRVNIAGVLASYLRNDRFPFIAATTFAGLHRYLEQNPSL
                     LSLFEVVEMSELSQEETLSVLEEVVPSFEGVYRKFVSFPALQAIVAYADRYIQAVPFP
                     KKAIDLLDEAMGYLATTSESILLPSHIALVVQERTQIPVGDIEGSEREVLLDLENLIH
                     KRIINQEEAVKEVASALRRARTDIATRKGPMGSFLFMGPTGVGKTETAKALAAIYFGS
                     EKRIIRLDMSEFQNVQDVERLLGTATQEGLLTSAVREDPFSLVLLDELEKAHPNILNL
                     FLQVLDEGHITDGLGRKTDFRHTIIIATSNAGYQLILEAVKTNADFAQLKQQLLDFVF
                     SQGIYRPEFINRFDGVILFTPLSKQHLMKIAGLMLQKLQSNLREKGIEF"
     gene            complement(2345..2731)
                     /locus_tag="VFE94_01600"
     CDS             complement(2345..2731)
                     /locus_tag="VFE94_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49830.1"
                     /translation="MMHSLLFLKYIEWHFIAAPKEILKGWGNILWFGFNYFSVPLLLK
                     TLFAPWRRITWSYGRGFDPGRYLFIFSSNLISRILGAIMRTFLIVAGIGLGLCLVVLG
                     GTFFVFWFLLPALIAIAFMYGIFLLF"
     gene            complement(2728..3543)
                     /locus_tag="VFE94_01605"
     CDS             complement(2728..3543)
                     /locus_tag="VFE94_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49831.1"
                     /translation="MKRLILFVAIAAILGGGIYYVTQVRPAQPSHQEQTLQEGTENGP
                     SGPEYFEPSETLARNIIAGALDIKFNAKTANYQVADLDGQGGPELIVAATENIPDLYA
                     RPSTAIIQVLSVKNREGEYESIGHVEYTELLWGTPEVKEVRDLNGDGDKEIFMELMYG
                     GAASFAQGVLTVDIQRKEVGWAKLQEKSRAMGDALFIVAASAMHWNTVAFTDTDGDGR
                     GEITTVFAQRDVFNPADVACQVEVYEWNESLFAYAEQLSLQTANTLGAECRLP"
     gene            complement(3604..3675)
                     /locus_tag="VFE94_01610"
     tRNA            complement(3604..3675)
                     /locus_tag="VFE94_01610"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3641..3643),aa:Val,seq:cac)
     gene            complement(3713..4321)
                     /locus_tag="VFE94_01615"
     CDS             complement(3713..4321)
                     /locus_tag="VFE94_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49832.1"
                     /translation="MKRLRIAIIGIEGSGKSWLIGKALETLGDRGSTITFSRIPGIQA
                     GGRIILRTWRWAHAKHYKLPIILLAALVTVLYGLAQWRMRKVEVLFLEHYPLIDLPAF
                     GALYANRAGSFLGHMVAWIFPKPNIILFLDIPIDVAEKRIRERGKPIQLYESCVSLAK
                     LHDLLRERALSFPNSFLCNALSPDLPKLLQDIMACSLKETVP"
     gene            4411..>5588
                     /locus_tag="VFE94_01620"
     CDS             4411..>5588
                     /locus_tag="VFE94_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49833.1"
                     /translation="MKIFEFQFNPKNKKDRFLKVFSFEPENRRQEQMGSLYMVAELDN
                     ALPSNSGFLERLAGIIKERYYESANDKSRQRVLAPEAALKQGLQSANDFLAQETKQGN
                     VDWLGNLHFALFLVAPKAGSLVLYFTKVGATRVWMGRHGSLVDVGKTIESARAEQVSP
                     KVFGAVGSGRMAPGDRLFVLTPELFEAFAKTNLLQGVARLQEEKEFKDLFKTKEKELS
                     AVSGALFFLLTEAVQLPDEEGRKSGALANPLSVLSQLSAPRLPSFSNIRLPGASFPKL
                     PKIPLALPFPRKKLPFTFSISDQAKRVGILMSFLFLLLLGFAIFGAAPQALQQDTGEK
                     LTQAQALFEEAEAALILREQSRANALLQQAWQLVNGLEGEEFVALRSELEGKLAELNK
                     "
BASE COUNT         1415 a         1342 c         1401 g         1430 t
ORIGIN      
        1 aaactcaata cccttttccc gcaggttgct ttgcagtttt tgcagcatga gtcccgcgat
       61 tttcataaga tgttgtttgc tcaaaggcgt gaacagaata acgccgtcga accggttaat
      121 gaactcgggg cggtaaatcc cttgggagaa aacaaagtca agcagctgct gtttgagctg
      181 cgcaaagtca gcgtttgttt ttaccgcttc taaaataagc tggtagcccg cgttgctcgt
      241 tgcaataata atagtatggc gaaagtcggt tttgcggccg agcccgtcgg taatatgccc
      301 ttcgtcgagc acctgcaaaa acaggtttaa aatgtttggg tgagcttttt cgagttcgtc
      361 gagcaacacg agcgaaaaag gatcctcgcg gaccgcactg gtaagtagcc cttcttgggt
      421 ggccgtaccc agcaatcgct ctacatcctg gacgttctga aattccgaca tgtcgaggcg
      481 tataatgcgt ttttccgaac caaagtaaat ggcggcaaga gcttttgcgg tctctgtctt
      541 tcccacccct gtcggtccca taaacagaaa actgcccata gggcctttgc gcgttgcaat
      601 gtcggtgcgc gctctccgca acgccgaggc cacttctttt acggcttctt cctggtttat
      661 aattcttttg tgaataagat tttccagatc gagcagaact tcgcgctctg aaccttcgat
      721 atctcctacg ggaatttgcg tgcgctcctg cacgaccagg gcaatgtgtg agggaagcaa
      781 aatgctttcg gaagtggtgg ccaggtagcc cattgcttcg tcgagcaggt caatggcctt
      841 tttgggaaaa ggaactgcct gaatgtaacg gtcagcgtac gcaacaatag cctgcagcgc
      901 agggaatgaa acaaacttcc ggtacactcc ctcgaatgaa ggaacgacct cttctaacac
      961 gcttaaggtt tcttcctgcg agagttccga catttccaca acttcaaaga gtgaaagcaa
     1021 agaaggattt tgttctaaat acctatgaag gccggcaaat gtggtagcgg cgataaaggg
     1081 gaaccggtcg ttccgcaaat acgaggccag cacccccgca atatttactc ttcctgcggc
     1141 ctgttccaag ccttcaccgg taaagttgtg gaattcgtcg ataactaaaa tggtgttccc
     1201 tgcagccccc acttccttaa aaatctggtt gagcgcggcc tctgcttctt ccaaactttg
     1261 caggccggcc aacaaatgcg taagctccag ttctaccacc cgctggtagt ttagcaaagg
     1321 gctttcgtgt tcccccaaga cgcttttgga agcaaaatcc tgaatgaggc gctttcttcc
     1381 cgttcccgga ttccccacca aaagcacgtt gttcatttgt tggcgcgaca gaatgcgtgc
     1441 catcatttct ttttcttgga tatgcccgac tgcccggggg aagcgaaggg tccgtacttc
     1501 gtcggaaata tcgtaagaga actggtctaa ggtaatggta tatcctgcgg cccagtcttt
     1561 gcccatggtg ccgtatcttc gcaaactgtt tttgagccag aacttttttc tggtcgccgc
     1621 ttccacccgc aggcggtagt gccagtctgc aatacgggcc atgtcgcgct ctggcaaaaa
     1681 gttgtgctca attaaaaaac ggttaaaaaa ttggtttgta tttgcgagcg ccgcaaacaa
     1741 atcctcgatt tcggcgcgct gcctgccttt ttcctgggcg taggctatgc ttttggttat
     1801 ggattcttca aagtctggag cgtataccgt ttcttttact tttcccggat ggcgtacctg
     1861 gcttgaaaga aggcgctgta cgtctttttt gaattcatta aaattgagca atgctcttgc
     1921 gaatataaac tcaatggacg cattttcttt gagcagaaag tataaaagca aagtagagtc
     1981 tacctgcata aagtttttcc ttttagcgta ggttgcagcc aagtgagccg ctttcgcaac
     2041 ctcaaaagaa acagcgttgg caaggttatg ccttaccaaa gggactttag gggctttgag
     2101 gtattcgcca aagcgctcta agagcaaaat cgtagttccg taagaaaacg tgagcaaagc
     2161 agctgccagc aagaggcggc tcactgtggg cgatacgatg cctccgcccg gaataaaggt
     2221 aaggagaaat gccagaaaag ccgcaagaaa aacccactgg gtgattttgc gcagtttgcg
     2281 cagggtagac gtgcgaaaat aattttcaaa cttgagcgcc gcccgcagtg ttgattgggc
     2341 tgcgttaaaa gagaaggaag atgccataca taaaagcgat agctataagc gcgggaagca
     2401 gaaaccaaaa cacaaagaaa gttcccccca gcaccactaa gcaaagcccg aggccgattc
     2461 ccgccacaat aagaaaggtg cgcataatag cgcccagtat tcgcgaaatg agattcgacg
     2521 aaaatataaa aagataacgc ccgggatcga atcctcttcc gtaagaccag gtgattcttc
     2581 gccagggcgc aaaaagcgtt ttgagcagca agggaacaga aaagtagtta aagccaaacc
     2641 aaaggatatt cccccaccct ttcaaaattt ctttgggagc tgcaataaaa tgccactcga
     2701 tgtattttaa aaaaaggagg gaatgcatca tggaagtctg cattcagcgc ccagggtatt
     2761 cgccgtttgc aaggaaagct gctctgcata ggcaaaaagg ctctcattcc attcgtacac
     2821 ttctacctgg catgcaacgt ctgcagggtt gaatacgtcg cgttgcgcaa atacggtggt
     2881 gatttccccc ctaccgtccc cgtcggtgtc ggtaaaggct accgtattcc agtgcatcgc
     2941 agaggcggca acaataaaaa gcgcgtcccc catcgcccgg gacttttcct ggagtttggc
     3001 ccagccgact tcctttctct gtatatctac tgtaagcact ccttgggcaa agcttgcggc
     3061 tcccccgtac attaattcca tgaaaatttc cttgtcgccg tcgccattca aatcgcgcac
     3121 ttcttttact tcgggtgtac cccagagtaa ctctgtgtat tctacgtggc caatgctttc
     3181 gtactcccct tccctgtttt ttaccgagag tacctgaata attgcggtgc tggggcgtgc
     3241 gtacaggtct ggaatgtttt ctgttgccgc aacaataagt tcaggccctc cttggccgtc
     3301 gaggtcggca acctggtagt ttgctgtttt cgcgttgaat tttatgtcga gagcgccggc
     3361 aataatgttc cgggctaagg tttcagacgg ttcaaagtat tcggggcctg acggaccgtt
     3421 ttcagtgcct tcctgcaggg tttgttcttg gtgggaaggc tgggcggggc gtacctgcgt
     3481 tacatagtaa atcccccctc ccaaaatcgc cgcaatcgcc acaaaaagaa tcagccgttt
     3541 catattctat atattaccag aacgagtgag aaataccaaa gggtgggtac gcgtattata
     3601 atgtgcaccc tgtaggattt gaacctacga cttcttgcgt gtgatgcaag cgctctgcca
     3661 ctgagctaag ggtgcattct tatcttataa aataaaagag actgtttgtc cattatggga
     3721 cagtctcttt gagggaacag gccattatat cttggagtaa tttgggcaga tcgggggaaa
     3781 gcgcattgca caagaagctg ttcggaaagg aaagggcacg ttctcgcagc aaatcgtgca
     3841 gctttgctaa tgagacacaa ctctcatata gttgaattgg tttgcccctt tcgcgaattc
     3901 gcttttctgc aacgtcgatg gggatatcca gaaagagtat tatgttaggt ttaggaaata
     3961 tccacgcaac catatggccc aagaaagaac ccgctcgatt ggcgtacaga gcgccgaagg
     4021 ctggcaaatc gataagcggg taatgttcta ggaatagaac ttccactttt cgcatgcgcc
     4081 attgcgccaa tccgtaaagt actgtgacaa gagcagcaag cagtattatg gggagcttat
     4141 aatgttttgc gtgtgcccac ctccatgtgc gtaagattat cctgcctcca gcctgaatgc
     4201 ctggtatgcg agagaaggta atggtgctgc ctctatcgcc aagggtttcc agagcctttc
     4261 caatgagcca actttttccg cttccctcga tgccgattat tgcaatccgt aatctcttca
     4321 aggaacacct cgttttggca gactgtggag attcttatag cattttttgg acagaaattc
     4381 aacaatcttc tataataaag cattcgcatc atgaaaatat ttgagttcca gtttaatccc
     4441 aagaacaaaa aagacaggtt cttgaaagta ttctcctttg agccggaaaa ccgtcgccaa
     4501 gagcaaatgg ggagtttata tatggtggca gagctcgata atgcgcttcc ctcaaattcc
     4561 ggatttttag agcgcttggc cggcataatc aaggaacgtt actatgaaag tgcaaacgac
     4621 aaaagcaggc aaagggttct tgctcccgaa gctgctctca agcagggctt gcaaagcgcc
     4681 aatgattttc ttgcgcagga aacaaagcaa gggaatgtgg attggcttgg caatcttcat
     4741 ttcgctctat ttcttgtagc gcccaaagca ggttccctgg tgctgtattt taccaaagtg
     4801 ggcgccacgc gagtatggat ggggcgccac ggctctttag tggatgtggg gaaaactata
     4861 gaaagcgcaa gggcagaaca agtttcgccc aaagtattcg gggccgtggg gtctggccgc
     4921 atggcgccgg gcgatcgttt gttcgtactc acgccggaat tgttcgaggc ctttgcaaaa
     4981 accaatttgc tgcagggggt ggcgcgtttg caggaagaaa aggaatttaa agacctgttc
     5041 aaaaccaaag aaaaagaact ctcggctgtt tcgggagcgc tctttttcct tctcacagag
     5101 gcggtgcagc ttccagatga ggagggccgc aagagcggag cgcttgcaaa tccgctgtcg
     5161 gtgctttcgc aattgtctgc gccgcggctt ccttcgtttt cgaacatacg cctgcctggc
     5221 gcgtctttcc ccaaactgcc aaaaattcct ttggcgcttc cctttccaag aaagaaattg
     5281 ccctttacct tttcgatatc agatcaggca aagcgagtgg gcattttaat gtctttcttg
     5341 tttcttttgc ttttgggctt tgcgattttt ggagcagcgc cgcaagccct gcaacaagat
     5401 accggagaga aacttaccca ggcgcaggcg ctttttgaag aggcagaggc cgcgcttatt
     5461 ttacgagaac agagcagggc aaacgcgttg ttgcagcagg cgtggcagct ggtaaacggg
     5521 ttggagggag aagaatttgt cgcgttgcga agtgaactcg aaggcaaact cgctgaactc
     5581 aacaaaat
//
LOCUS       DASRUP010000039         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_251864, whole genome shotgun sequence.
ACCESSION   DASRUP010000039 DASRUP010000000
VERSION     DASRUP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251864"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..704
                     /locus_tag="VFE94_01625"
     CDS             144..704
                     /locus_tag="VFE94_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZX49834.1"
                     /translation="MSNKGFTLIEVLVAFFLFVLVMGGAFSGMQQAITVQRDALEKRA
                     AVEELSFAMEYLARALRGAQKDLAGDCLPAGYTFEATTRIRWLDRNNRCREIFSDAGH
                     DFSERISSDSTPSFGTPRGLISNDFDADVFWFRVFGEYQGDAVDDPDYIQPHVTIGVL
                     LRSNHDNRELVSMQTTISTRVIDVEQ"
     gene            706..1803
                     /locus_tag="VFE94_01630"
     CDS             706..1803
                     /locus_tag="VFE94_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX49835.1"
                     /translation="MQKGSAIYFAIVITSLIMAIALGLSTLLLGGFGILQGSVKSAIA
                     FYAAEAGVEHILFEDKTCAGDINCLQIAAASWGLGGVVTLSNQATYSVDVKDPGDCVG
                     APFCALSTGIFQNVARTIEVTRQIFLLPCGEEIDVALALDMSDSIDDSDLVALKDAVS
                     TGPVNFIDILDPTPAGTHMSVVIFQNLATIVEHLTGNNSNLHTTVDSLGFGGPPPSGF
                     GGTNLQDGIYDSTQIELANPGDGHDRPDATSPDYLILVSDGDPTNYLDPNVDCFPEHD
                     HAEGPAEPDPSYKACKQAARDEALAEAVAAKGNGITIFVLGVDIVGTEGFTAAENEDL
                     LRSIASSPAHYASVDDYSVLETALANLCPAP"
     gene            1824..3809
                     /gene="ligA"
                     /locus_tag="VFE94_01635"
     CDS             1824..3809
                     /gene="ligA"
                     /locus_tag="VFE94_01635"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZX49836.1"
                     /translation="MTKAEAQKRIEKLRQVINRQRYLYHVLDKQDISDEAHDSLKHEL
                     WELERQFPDLVTPDSPTQRVGGEPLDKFEKVEHIQPMLSIEDIFGEEELRDWEEHLLR
                     LRGGTVPPLEYFAELKVDGFAVSLRYRNGVLETAATRGDGKVGEDVTQNVKTIESIPL
                     RLETQNSTFEIPEELEVRGEVYMEKKAFEKFKSSFANPRNLAAGSIRQLDPKLAASRP
                     LTFMAYDLVGNFGQGSHAKEHELLGKLGFKTDPTAKVCRNTQEILAYWKAMVTKRDSL
                     EFHIDGIVAQVNDNATLQELGVAGKGYRGMRALKFAGKQATTKILGIQVQIGRTGAVT
                     PVALLRPVQVAGVTISRATLHNEDELQRLGVKIGDTVVVERAGDVIPAVVQVLKDLRA
                     GKEKKFAMPKICPMCKSKLVKPAGEAVTRCSNKNCQAQQRQYLKHFVSGKAFDIEGLG
                     PKIINQLAEQGIVRDAVDLFEITEGDLVPLERFAAKSAKNVVDAIRAQKEISLERFVY
                     ALGIRHVGEETAIDLAERYGSIEKLQQASKEELEQIRDVGGVVAESMYKWFHAKENIE
                     FIRRLQKSGVRIHNTQYLIHNTKLKGKTFVLTGQLDSMTREEAKEKIRQLGGEVAESV
                     SSKTSYVVVGREPGSKFETAKKLGISLLGEKEFLKLV"
     gene            3834..4916
                     /locus_tag="VFE94_01640"
     CDS             3834..4916
                     /locus_tag="VFE94_01640"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZX49837.1"
                     /translation="MWDHLREFDWLLVGSSFVLFAIGMLALHSSSLARGDFGNFEKQL
                     IFGGIGISCMFIVSFFDYRLLRNDSLLILGLYGIGVAALVGLLLFAPETRGVKSWYKI
                     GDISVDPIEYMKLILVILMAKYLSLRHFELYRIRHIVITGAYFSVPMFLIALQPNLGP
                     VLVLFALWVFMLLVSGIRIRHFLAILTVVVLIFALGWTFFLLDYQKARILSFVEPELN
                     PLTIGWSQLQAKIAIGNGGMLGQGYSQGTQTQYGFLSEPQTDFIFAALAEEFGLLGIS
                     VLLFFLILFVWRIFKIGAQAQTNFARLFAIGFAVLLIVQTFINIGMNLGLLPIIGLPL
                     PLVSYGGASLIFTYIGLGVLLSIKTH"
     gene            4993..>5371
                     /locus_tag="VFE94_01645"
     CDS             4993..>5371
                     /locus_tag="VFE94_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49838.1"
                     /translation="MPIGQVISPQKPSQEYITLSQAATRCPYSQEYLSLRARQGKLKA
                     AKIGCKWFTTSEWLQEYAEKTEVYKKHVFEARLQTPSNPPTNLPIFVSEIDAAEDPTP
                     EEMEQRKTFQRKAGFAIAFAMVLL"
BASE COUNT         1393 a         1163 c         1419 g         1396 t
ORIGIN      
        1 ggagtggttt catcgtttac aagggttgtt actgttgact ataatggaga cgaggttacg
       61 gtaaaggtaa cagtatcctg ggatgaaaag ggaaatcccc aaagcgtcgt tacagaaaca
      121 acattgtttg attggctgcc attatgagca acaaagggtt tacgctcatt gaggtacttg
      181 tcgcgttttt cctgtttgtg ctggttatgg gaggcgcgtt ttccggcatg cagcaggcaa
      241 ttacggtgca gagagacgcg ttggagaagc gggcagcggt agaagagttg tcgtttgcaa
      301 tggaatatct ggcgcgggcg ctgcggggcg cgcaaaaaga tttggctggc gactgtcttc
      361 ccgcagggta cacgtttgaa gcaaccacca gaatccgctg gttagacagg aacaacagat
      421 gccgcgagat tttttcggac gccggccacg attttagtga acgtatttcc agcgacagca
      481 ccccgagttt tggaaccccg agggggctta tttccaacga tttcgacgct gatgtgttct
      541 ggtttcgggt attcggggaa taccaggggg atgcggtaga cgacccggat tatatacagc
      601 ctcacgttac gattggggtg ttgttacgga gcaaccacga caatagagag ttagtaagca
      661 tgcaaacaac aataagtacc cgtgtcatcg atgtggaaca ataacatgca aaaaggatcg
      721 gcaatatact ttgcgatagt cattacttcc cttataatgg ctattgcgct tggattgagc
      781 acgctgctgc ttggcgggtt tggtattctg cagggaagcg tgaaatctgc gattgctttt
      841 tacgccgcgg aagccggagt tgaacatatc ttgtttgaag acaaaacatg cgctggcgat
      901 ataaactgtt tgcagattgc ggctgccagc tgggggcttg ggggagttgt tactctttct
      961 aaccaggcta cctacagtgt tgacgtgaaa gaccccggag actgcgtagg cgctcctttt
     1021 tgcgctttgt caacgggaat attccaaaac gtagcccgga ccattgaagt aacccgccaa
     1081 atctttttgc tgccgtgcgg cgaggaaatt gatgtggcgc tcgcacttga tatgtcagac
     1141 tccatagatg attctgacct ggtggccttg aaagatgcag taagtacggg gcccgttaac
     1201 tttatagata ttttagaccc tacgcctgcg ggaacccaca tgagcgttgt tattttccag
     1261 aacttggcaa ccatagtgga acatttaaca ggcaataata gtaatttgca tactactgtt
     1321 gattctcttg gctttggagg ccctcctcct tccgggttcg gaggaacgaa tttgcaagac
     1381 ggaatttacg attccacaca aatagaactt gcaaaccctg gcgacggcca tgaccggccc
     1441 gacgcgactt cgccagacta cttgattctg gtgtcagacg gagaccccac taactattta
     1501 gatcccaatg tggattgttt tcctgaacac gatcatgccg aaggccctgc ggagccagat
     1561 cccagttaca aagcctgcaa gcaagcggcg cgggatgagg cgctcgccga ggctgttgca
     1621 gccaagggaa acggcattac gatttttgtg cttggcgttg atattgttgg tacagaaggg
     1681 tttacggcgg cagaaaatga ggatctttta cgcagtattg cttcgagccc ggcgcattac
     1741 gcctctgtgg acgattatag tgtcttggaa accgcgctgg caaacctgtg cccggcgcct
     1801 tagattgtgc tagaataggg ctaatgacaa aggcagaggc ccaaaagcgc atagagaagc
     1861 tgcgccaggt tataaaccgc cagcggtatt tgtaccacgt attggataag caggacattt
     1921 cagacgaggc gcatgattct ttaaagcacg aactgtggga actggaacgg cagttcccag
     1981 atttggttac gccggattcc ccaacccagc gcgtgggagg cgagcctttg gataagtttg
     2041 aaaaggtgga acatatccag cccatgctct ccatagaaga tatatttggc gaggaagaac
     2101 tgcgagattg ggaggaacat cttttgcgtc tccgaggagg gactgtccct cctcttgagt
     2161 attttgcaga gctcaaggtt gatgggttcg cggtttcttt aaggtaccgg aacggggtgt
     2221 tggaaacggc ggcaacgcgc ggcgacggca aagttgggga agacgtaacc caaaacgtaa
     2281 aaaccattga aagtattccg ttgcggttgg agacgcaaaa ctcaacattt gaaattccag
     2341 aggagctgga ggtacggggc gaagtatata tggaaaagaa ggcgtttgaa aagtttaaat
     2401 cttcctttgc caacccccgc aacctggccg cgggctccat tcgccagtta gacccaaaac
     2461 ttgccgcttc ccgtcctctt acctttatgg cctacgacct tgtaggaaat tttggccagg
     2521 gttcccacgc aaaagaacac gagctgctgg gaaaattggg ctttaagacc gaccctaccg
     2581 cgaaagtgtg ccgcaacacg caagaaattt tggcgtactg gaaagcaatg gttacaaagc
     2641 gcgactcttt ggagttccac atagacggta tcgtggcgca ggtaaacgac aatgccacgc
     2701 tccaggagct tggagtggcg ggaaaagggt atcggggcat gcgcgcttta aaattcgcgg
     2761 gaaagcaggc caccaccaaa attttaggca tccaggtgca aattggcagg accggggcgg
     2821 taactccggt ggctctgttg cggcccgtcc aggttgcggg tgttaccatt tcaagggcaa
     2881 ccctgcataa cgaagacgag ttgcaacgcc tgggagtaaa aattggagat accgtagtag
     2941 tagaacgggc cggcgatgtt attccggctg tagtgcaggt actaaaagat ttgcgcgccg
     3001 gaaaggaaaa aaaattcgcc atgccaaaaa tttgccctat gtgcaaaagc aagctggtaa
     3061 agccggcggg ggaggcggta acgcggtgct ccaataaaaa ttgccaggcc cagcagcgcc
     3121 agtacctaaa acattttgtt tctggcaaag cgtttgatat tgaaggtttg ggccccaaga
     3181 ttattaacca gcttgccgag cagggaatag tacgggacgc ggttgacttg tttgagatta
     3241 ccgaaggcga tttggtcccg ttagagcggt tcgccgcaaa gtcggcaaaa aatgtagttg
     3301 acgcgatacg cgcgcaaaaa gaaatttccc tggaacgttt tgtgtacgcg ttgggcattc
     3361 gccatgtggg cgaggaaacg gcaattgacc tggccgagcg atacggaagc attgaaaaac
     3421 tgcaacaagc atcaaaagaa gagttagagc aaatccgcga tgttggcggg gttgtagcag
     3481 aaagcatgta taagtggttt cacgcaaaag aaaatattga gtttataagg cgtttacaaa
     3541 agtccggcgt ccgtatacat aatacacaat acctaataca taataccaaa ctgaaaggaa
     3601 aaacatttgt gcttacgggt cagctggact ccatgacgcg cgaggaagcg aaagaaaaaa
     3661 tccgccagtt aggcggcgag gtggctgaat cggtatcgag caaaacttcc tatgttgtag
     3721 ttggcaggga acccggctcc aagtttgaaa cagccaagaa actcggcatt tctctactcg
     3781 gagaaaaaga attccttaaa cttgtttaaa agtagcaaat gaatacgcga ggcatgtggg
     3841 atcatttaag agaatttgat tggctgcttg ttggcagctc atttgttcta tttgcaattg
     3901 gaatgcttgc gcttcacagc tcgtctctgg caagaggtga ttttggcaat tttgaaaaac
     3961 agctcatttt tggaggtatc ggcatctctt gcatgttcat tgtttcgttt tttgattatc
     4021 gacttttgcg gaacgattct ttgctcattc ttggtttgta tggcattgga gttgccgctt
     4081 tagttggctt gcttcttttt gctcctgaaa cccgcggtgt gaagagctgg tataaaatag
     4141 gggacatatc ggttgatccg atagagtata tgaagcttat cttggtaatt ttgatggcaa
     4201 aatacctttc tttgaggcat tttgaactct atcggatacg gcatattgta atcactggag
     4261 cttatttttc agttcccatg tttttgattg ccctgcaacc gaatcttggg cccgtgctgg
     4321 tactttttgc tttatgggtt tttatgcttt tggttagcgg tattcgcata cgacatttcc
     4381 tggcaatttt gacagtagtt gtgctcattt ttgctttggg ttggacattt ttcctgcttg
     4441 attatcaaaa ggcgcgaatt ttaagctttg tggaacctga gctcaatccc ttgactattg
     4501 gttggagcca attgcaggca aaaatcgcta ttgggaacgg cggcatgctc ggccagggat
     4561 atagccaagg cacacaaacc cagtatgggt ttctctctga accccaaacc gactttattt
     4621 ttgccgcact tgcggaagaa tttggcctgt tgggcatctc tgtcttactt ttcttcctta
     4681 ttctctttgt ctggcgaatt ttcaaaatag gcgcacaagc tcaaaccaac tttgcccgac
     4741 tttttgccat aggctttgcc gtattgctta ttgttcagac atttatcaac attggcatga
     4801 atctgggtct tttgcctatc attggcctgc cactcccact ggtaagctat ggaggcgcat
     4861 ccctcatttt tacctatatc ggcttgggtg ttttactaag tatcaaaact cattaatcag
     4921 ctttaaccat tctatcggat attcacaggt agaggcattg acctgcttat tttgtattgg
     4981 gataatgtgc taatgcctat cggacaagta atttctccgc aaaagccttc gcaagaatat
     5041 atcactcttt cgcaagcagc aacacgttgt ccttactcac aggaatattt gagtttgcgc
     5101 gcgcgacaag gaaaactcaa agcagcgaag ataggctgta aatggtttac cacaagcgag
     5161 tggctgcaag agtacgcaga gaaaacagaa gtatacaaga agcatgtgtt tgaagccaga
     5221 cttcaaacgc caagcaatcc gccgacaaac cttccgattt ttgtttccga gatcgacgcg
     5281 gcggaagacc ctacgccaga ggaaatggaa cagagaaaaa cttttcaaag aaaagcaggc
     5341 tttgcaattg catttgccat ggtgttgctt g
//
LOCUS       DASRUP010000040         4760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1542012, whole genome shotgun sequence.
ACCESSION   DASRUP010000040 DASRUP010000000
VERSION     DASRUP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542012"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFE94_01650"
     CDS             complement(<1..1025)
                     /locus_tag="VFE94_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HZX49839.1"
                     /translation="MVELIRRAKGNAVFRNVVWLGMGEVVSRLFLFALLIAIARVLGV
                     HGYGMFAFGLSVASLVLVVAQFGIHAIFVRDATQDQKWEHAFPAFLTLSILSGLGGFL
                     AMAGAAFFLSKETSIHVLFLFLGGYVAAMTVLEMMYAFFRAREEMRYEAVLKVLQAVL
                     TVSLGAILLLLAPTSANVGLAYAGGAASALLVMIWIFWFRTRSIDLQISLPLFKTVLS
                     RTWPLGLAGVFAVVYNSIDSVMLGALGQVAAVGWYNAAYRIVGAALIPAVFLGHSFLP
                     ALSKRLGSSIGSLQNLWERQVEVALMLGVPASVGGAIMAPRIIRALYPPEFDPAILAL
                     QILIWTVA"
     gene            complement(1018..1932)
                     /locus_tag="VFE94_01655"
     CDS             complement(1018..1932)
                     /locus_tag="VFE94_01655"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX49840.1"
                     /translation="MKTYIVTGGAGFIGSHLADLLVQRGERVVVVDNLSTGKKQNLNR
                     KAKFLNMDIRSPRLGQLFLRERPHGVFHLAAIPRVPISVEDPVGTSDVNITGTVNVFW
                     AAKKAGVKRVVFASSSSVYGSQKNLPLHEAMQPKPVSPYGLQKLMGEQWARVFFELYG
                     LPIVSLRYFNVYGPRIDFDSDYGLVIGKFLWQFRQKKPLTIFGTGNQTRAFCYVDDVA
                     QATVLAMQSLKLKGADIINVGGEESHSINHLAALLGGQKEHLPPRPGDVEHTKADTKA
                     AKRLLGWEQTVSLKEGVRRTAEWFKQNG"
     gene            complement(1985..3913)
                     /gene="asnB"
                     /locus_tag="VFE94_01660"
     CDS             complement(1985..3913)
                     /gene="asnB"
                     /locus_tag="VFE94_01660"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZX49841.1"
                     /translation="MCGIAGIRDFGGSTRSQGEVVGRMTSCLAHRGPDDKGVFVDEAE
                     GVALGHRRLSILDLSSQGHQPMATPGGQLWITYNGEIYNFQELKNELAKRGYTFESDT
                     DTEVILKAYEEWGMGMLDKFHGMFAFALWDKKTRKLTLCRDRAGVKPLYYYWRDGLLL
                     FASELKSLHEHPAFEKKLNLQALSLFFQFGYIPAPHTIFEHTFKVLPGSYLVFDEKGG
                     REERVYWDIRDLYATEQNHATEDDMVQELEAILKKAFAWRLVADVPLGVFLSGGIDSS
                     TVAALLQAQVSYPLKTFTIGFTEKGYDEAPYAKRIARHLGCDHHEVYCTKEYAAEIIP
                     KLPEMYDEPFGDSSAIPTYLVSQLARKHVKVALSADGGDELFGGYTKYDLVNRYWAKR
                     QRFPSALFSSAAFFLQMVSPEVAEQLYLLLPSSFRKYTNAKDKMYKLRNLLREQTLPD
                     IFRQSNTFWTEEQLRLLLAFAPPSRESDMYGFDKMRGLDPVSAMQAADFRVYLPDDLL
                     VKVDRATMAQGLEGREPFLDQDIASFIARVPSSWKVRSGKGKYILRKVLAKYVPPELF
                     ERPKQGFGIPIYAWMKQDFSRFLEEYLSASQLKKHGLFDEEAVQRILQRHAQGKGEHA
                     SKLWFLLMFQMWYSRWMQ"
     gene            complement(3919..>4760)
                     /locus_tag="VFE94_01665"
     CDS             complement(3919..>4760)
                     /locus_tag="VFE94_01665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX49842.1"
                     /translation="IYTIHGLFFLKDSSWRRKLAFGTVEKILSFLVYKALFVNREDME
                     EVQRMNIYGSNKITYIGADVDLSRFDPSLHSRDEIQKAKSELGIPLDKAVIGIVARLV
                     WEKGFADLFSAMQIILQKFPHVVLLVVGPSEPEKGDGFQPSVVETYGIQENVVFAGER
                     TDVPTMYALMDVFVLPSYREGLGLSALEASAMEKAVVATDIRGCRESVEHGRTGLLVP
                     PKSPEKLAQALEELLSDPERRKAMGKAGREKVQKEFAKEILFGRLQTIYSELMAHKHF
                     NRE"
BASE COUNT         1258 a         1303 c         1072 g         1127 t
ORIGIN      
        1 gccacggtcc agataagaat ctggagcgcc aaaatagcag gatcaaactc cggagggtac
       61 agggcgcgga taatacgagg ggccattatt gctcccccaa cgcttgctgg cacgccgagc
      121 atcaaggcta cttccacctg ccgctcccag agattctgaa gagaaccgat ggatgaacca
      181 aggcgtttgc ttaaagcggg aagaaaacta tggccaagaa acacggccgg aataagagcg
      241 gcacccacaa tgcgataagc ggcattgtac catccaacgg ccgcaacctg gccgagtgcc
      301 cccagcatta cagaatcaat ggaattatac acaacggcaa acaccccagc taatcccaaa
      361 ggccatgtgc gagagagcac ggttttgaaa agcggaaggc taatctggag gtcgatggaa
      421 cgggtacgaa accaaaatat ccatatcatt accaaaagcg cgctggcagc cccccccgca
      481 tatgcaagcc caacattggc gctcgtgggc gcaagcaaga ggagaatcgc gccaagggaa
      541 actgtcaaaa ccgcctgaag tactttcaaa acagcttcat aacgcatctc ttcccgtgcg
      601 cgaaagaagg catacatcat ttcaagaaca gtcatggccg ccacgtatcc gcccaaaaag
      661 agaaataaca catgtatcga ggtttctttg ctcaaaaaga acgcggcgcc ggccatcgcc
      721 aagaaccccc ccagtcccga gagaatactc aacgtcagaa atgcagggaa ggcatgttcc
      781 cacttttggt cttgcgtcgc gtctcgtaca aagattgcat gaataccaaa ctgcgcgaca
      841 acaagcacaa gagatgctac agaaagccca aacgcaaaca ttccgtagcc atggacgcca
      901 agcacacggg caattgcgat caacagggcg aacaggaaca aacgcgatac cacctctccc
      961 attccaagcc aaaccacatt ccgaaatacc gcattcccct tggcgcgtcg gatgagctca
     1021 accattctgc ttgaaccatt ctgccgtgcg ccgtacacct tctttgaggg acactgtttg
     1081 ttcccaacca agaagacgtt tagcagcctt ggtgtctgct ttggtgtgct ccacgtctcc
     1141 cgggcgaggc gggagatgtt ctttttgtcc gccgagcaaa gccgcaagat gatttattga
     1201 gtgcgattct tccccgccca cgtttataat atcggctcct ttgagtttca aggactgcat
     1261 agcaagcacg gttgcttgcg ccacgtcgtc aacatagcaa aaagctcttg tttgattccc
     1321 ggtgccaaaa atcgtgagtg gctttttttg ccggaactgc cacaagaact ttccaatcac
     1381 caagccatag tcagaatcaa aatcaatacg cggaccatat acattaaaat atcgcaaaga
     1441 aacgatgggc agtccataca gctcaaaaaa taccctggcc cactgctcgc ccatcagctt
     1501 ttgaagccca tacgggctta cgggctttgg ctgcattgcc tcgtgtaaag gaaggttctt
     1561 ttggctgccg tataccgaag aagacgaggc aaacactacc cgctttaccc cagctttttt
     1621 tgcagcccaa aaaacattca cggtacccgt gatattcacg tccgaggtgc ctacgggatc
     1681 ctccacagat atgggcacgc ggggtatcgc agcaagatgg aaaaccccat gagggcgttc
     1741 ccgcaaaaag agttgcccca aacgaggact ccggatatcc atatttaaaa atttcgcctt
     1801 gcgattgagg ttttgttttt tgcccgtgct caagttatct acgaccacta cccgctctcc
     1861 cctttgtacc agcaaatctg caagatggct tccgataaat ccagctcccc cggttacaat
     1921 atatgttttc ataggtgtta ttgcgaatcg tttattattg caggaaatct gtttctcttc
     1981 aaactcactg catccaacgg gagtaccaca tctgaaacat cagcaaaaac cacagcttgg
     2041 aggcatgttc cccttttccc tgtgcatggc gctgaagtat tcgttgtacg gcctcctcgt
     2101 cgaacaggcc gtgttttttg agttgggaag cgcttaggta ttcctccaga aaccgggaaa
     2161 aatcctgctt catccaggcg tatatgggga taccaaagcc ttgtttgggc ctttcaaaaa
     2221 gttctggagg gacgtacttt gcaaggactt ttcggagaat atatttccct ttgccagaac
     2281 gcactttcca ggaactcggc actcgtgcaa tgaacgaggc aatatcctgg tcaagaaaag
     2341 gttccctccc ctctaaaccc tgcgccatgg ttgctcggtc cacctttacc agcaaatcat
     2401 ctggaagata cacacgaaaa tctgctgcct gcattgcgga gactggatca agaccgcgca
     2461 tcttatcaaa cccgtacata tcactctccc gggagggcgg ggcaaaggcc aggagaaggc
     2521 gcaactgttc ttcggtccag aacgtgttgc tttggcgaaa aatgtcgggc aaggtttgct
     2581 ctcgtagaag gttgcgcaac ttatacatct tgtctttggc gttcgtatac ttcctaaagg
     2641 aagacggcag gagcaggtag agttgttctg cgacttcagg cgaaaccatt tgcaaaaaga
     2701 aagcggcaga agaaaagagc gccgagggaa acctctggcg ctttgcccaa taccggttta
     2761 ccaagtcgta cttggtatag cccccgaaca attcgtcgcc cccatctgca gaaagcgcca
     2821 ctttcacatg ctttcttgcg agttgcgaaa caaggtacgt aggaatggca gaactgtcgc
     2881 caaacggctc gtcgtacatt tcggggagtt tgggaataat ttctgccgca tactctttcg
     2941 tgcagtacac ctcatgatgg tcgcatccta aatgccgggc aatacgtttg gcatagggag
     3001 cttcgtcata ccccttctcg gtaaaaccaa tcgtaaaggt tttcaggggg taagacacct
     3061 gcgcctgcaa caacgcagca acggtcgagg aatcaatgcc cccgctcaaa aatactccta
     3121 agggcacatc ggccaccagg cgccacgcaa acgccttctt gagaatcgct tctaactcct
     3181 gcaccatatc gtcttctgtc gcatgattct gttctgtggc atataaatct cgaatatccc
     3241 aatacactct ctcttctctg ccgccctttt catcaaacac cagataggag ccaggcaaga
     3301 ctttaaaggt atgctcaaaa atcgtatggg gagcaggaat atacccgaac tggaaaaaca
     3361 gcgacaaagc ctgaaggttt agctttttct cgaatgccgg atgctcgtgc aaggacttta
     3421 actctgaagc aaagagcaaa aggccgtctc gccagtaata gtaaagaggc tttacccctg
     3481 cacggtctcg gcacaaggta agctttcgag tttttttatc ccacaaagca aacgcaaaca
     3541 tgccgtggaa tttgtcgagc atgcccatcc cccactcctc ataggctttt aggataacct
     3601 cggtgtcggt gtcgctttca aatgtgtagc cacgtttggc aagctcgttt ttcagttcct
     3661 gaaagttata gatttctccg ttgtaggtaa tccatagctg cccacccggc gttgccatgg
     3721 gctgatgccc ttgagacgat aaatcgagaa tcgagaggcg ccgatgcccc aacgccaccc
     3781 cttccgcttc gtcaacaaaa acgcccttat catctgggcc gcggtgcgca aggcagctcg
     3841 tcatgcgacc taccacctcc ccctggctcc tcgtgctgcc accaaaatct ctaattccgg
     3901 caataccaca catggctatt attcgcgatt gaagtgttta tgagccatga gttcactata
     3961 tatggtttgg agcctgccga acagaatttc tttggcaaat tctttttgca ctttttctct
     4021 tcctgctttg cccattgctt tcctccgttc cgggtcggag agcaattctt caagagcttg
     4081 cgccagtttc tccgggcttt ttggaggcac gagcaagccg gttcggccgt gctcgacaga
     4141 ttcccggcat ccccgaatat ctgttgctac taccgctttt tccattgccg aggcttccag
     4201 tgccgaaagg ccaagcccct ctctgtaaga aggaagaaca aatacatcca taagagcgta
     4261 cattgttggc acatcggttc tctcccctgc gaaaaccacg ttttcttgaa taccgtacgt
     4321 ttccactacc gagggctgaa agccatcccc cttttccggc tctgaaggac caactactaa
     4381 taacacgaca tgcgggaatt tttgcaggat aatctgcatt gcagaaaaca aatcggcaaa
     4441 ccccttttcc cacaccagcc tggccacaat gccgatcaca gccttgtcta aaggaattcc
     4501 gagttcgctt tttgcttttt ggatttcatc tctcgagtgc agactggggt cgaaacgaga
     4561 aaggtccaca tcggctccta tataagtaat cttgttagag ccgtagatat tcatcctttg
     4621 cacttcttcc atatcctctc tattcacgaa caatgcctta tacaccaaaa atgataaaat
     4681 cttttcaact gttccaaaag cgagttttct cctccatgag gagtctttta aaaagaaaag
     4741 cccatgaatg gtgtaaatac
//
LOCUS       DASRUP010000041         5645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1119314, whole genome shotgun sequence.
ACCESSION   DASRUP010000041 DASRUP010000000
VERSION     DASRUP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119314"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..847)
                     /locus_tag="VFE94_01670"
     CDS             complement(137..847)
                     /locus_tag="VFE94_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49843.1"
                     /translation="MSTKVFYRVVACVVSFVFLFGATSYAEAQTTTTGTGFEVAQKVR
                     NLTQQKFDWVDKVQASPQDRLEFQITVTWRGSATTENVLVREILSDKLSYAGNLRVDS
                     NQVAGNVTTENINIGSLNTNQSRTITFETLVVDAASLPAGTSNLVNTVTVFNTQGGAS
                     TTAQVQVARDGKPTDVSTGPLSLWQIGFALVLLVMGVASAVFFAKYYFTREVLQSPYE
                     NRTDRKLASMIEKGKRQF"
     gene            complement(866..1507)
                     /locus_tag="VFE94_01675"
     CDS             complement(866..1507)
                     /locus_tag="VFE94_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HZX49844.1"
                     /translation="METRVIKENLLYLGEEGISRYGRRALFVYLFLAASFGLMWAFQK
                     VDAYLTPSYAREENAPQNDTLSTTAPFEIKEASLTIPKIGVAAPVYFVDSSNPDDFLE
                     PLEKGVAHYPSAIPGQKGTAVLLGHSAPPGWLGSYFDGVFSDLTKLEQGDMLSVSING
                     VAHQYRVAGKTILHRGEDIPSDLMESEVSRVVLLSCWPPGIDNKRIMIQAEKI"
     gene            complement(1576..1959)
                     /locus_tag="VFE94_01680"
     CDS             complement(1576..1959)
                     /locus_tag="VFE94_01680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZX49845.1"
                     /translation="MKQFIIALAAVVLAGAVIWYGSFLKPQESMEQAEPGDTVAVHYE
                     GRLEDGTVFDSSRAKGAPIQFTLGAGDVIAGWDQGIQGMKVGEKKQLVIPPELAYGER
                     GFMDVIPPNATLLFDVELVDIQKGK"
     gene            complement(1956..2681)
                     /locus_tag="VFE94_01685"
     CDS             complement(1956..2681)
                     /locus_tag="VFE94_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZX49846.1"
                     /translation="MMNVSRSNQICFTGPRVEALLKMHPEEVQPERMSKILEEFVVGF
                     HFLRDFELAATFFGSGRVGAEHRTYLEAEKLAYTLAKDGFAIITGGGPGIMEAANKGA
                     YEAGGRSAGINIQLPQEQRTNRYVKEAEAFYYFFVRKVMLAFASEVYVFFPGGFGTLD
                     EFFELATLVQTGKIEPIPIILVDKGYWKPLLKWVEKELYEKRKAIGKEDKELYHLVDN
                     ADEAFKLIQKMVIRPREDEKKPL"
     gene            complement(2678..3826)
                     /locus_tag="VFE94_01690"
     CDS             complement(2678..3826)
                     /locus_tag="VFE94_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49847.1"
                     /translation="MKEKFCIAIIAIGVLFSPLLAATKNSSDEEGRKLVPSDMVITED
                     FGVAGETVEIAGTIQGDAYVAGAQVFVNGVIEGDLIVVGGTITITGKVTQNVRAMGGQ
                     VVIAGEVGRNVTAAGMNVTFADSAKVLGGIVVAAANVHLAAPVQKEARIAGRTLTLDN
                     AIEGDLEAAVDTIRFTSNAKVGGDFTYWSNREPLIAQGATIEGNTIKESPAELAAPIT
                     EVLSPIQPFIKPFLGLTSFVTTLILGWIFLHFFPGFTKGTVATLKTRKIKSLTTGVTV
                     LLLLPLLVGLLILTIVGIPLGLVLLLVSSLVLYVARIFAMILFGNIILQYFDGKENGM
                     LAFLIGLVLYSVFSFFPIVGWAISLGAVVFGLGAMVQTLRDQRAKKPA"
     gene            complement(3860..4762)
                     /locus_tag="VFE94_01695"
     CDS             complement(3860..4762)
                     /locus_tag="VFE94_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZX49848.1"
                     /translation="MKTTFVVVIVSAACILFALNLFLGVFGDVLPIAKLSPLFENAAE
                     GRGIFTQRRDFSLLVEELKQAVSTPAPLRSGEEAPTSFLTKQGVFQWTNTQRQEQGLS
                     LLEADTTLDNVAAARARDMFEKQYFGHVAPDGSGVAEAAQKEEYAFLAIGENLALGNF
                     ATDSALVQAWMDSPGHRENIVSPRYQEIGIAVEQGEFEGNTVWIAVQIFGLPSSACSM
                     PSSELESDIELKKIQIEEMEAILDKEYKEIEATEPKRGQVFRDRVDHYNQLVAQYNKL
                     VEDVQALIGEYNVEAREFNECANG"
     gene            complement(4804..>5645)
                     /locus_tag="VFE94_01700"
     CDS             complement(4804..>5645)
                     /locus_tag="VFE94_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZX49849.1"
                     /translation="KISITVIFTHMMNERIKDVLGVVVILGLVFFAYVIWVYVDAYSK
                     SIEPSSFRSFQISAEGKEVAIPDIAEFTFSVITEGGTNLQALQTQSSEKTNAAIEYVK
                     SQGVEAKDIKTQGYNVNPRYQYFDCKPRPLPIGEGVLVPEACPPAEIVGYTISQTVSV
                     KVRDLQKAGVLLSGVVDNGANNVSQLFFTIDDRDAVESRARAQAIEKAKVKAEAVAKA
                     GGFRVGRLLSIQESGSPYDPLYELDRALEQGIGGAAPSVAPKVEPGSQDVTVNVTLRY
                     EIQ"
BASE COUNT         1386 a         1544 c         1233 g         1482 t
ORIGIN      
        1 taccaaaaaa attgcaacgg tggtaacaaa aaagaaaatg tctgctttta caaatgtttc
       61 catatatttt attgtaccat tcttttgcaa caacaaacag ccccgacaat acgtcgaggc
      121 tgtggaaaac agagggttag aattgccgct ttcctttttc aatcatagag gctaactttc
      181 gatcggtgcg gttttcatac gggctttgca gcacttcgcg ggtaaagtag tattttgcaa
      241 agaatacagc gctcgcaacg cccataacca agagcaccag cgcaaatcca atctgccaca
      301 ggcttaaagg cccggttgaa acatcggtgg gcttgccgtc tcttgccacc tgcacctggg
      361 ctgtggtaga agctccgccc tgggtgttaa acacggtaac ggtgtttacc aggttggaag
      421 tacccgccgg caaggacgct gcatcaacca cgagtgtttc aaatgttatt gttcgcgact
      481 ggttggtatt taaacttcct atattaatgt tctctgtggt aacgtttcct gctacctggt
      541 tgctatcgac ccttaagttg ccagcatacg agagcttatc tgaaagaatc tcccgcacca
      601 gcacattttc cgtggtggcc gatccccgcc acgttacggt aatctgaaac tccaggcgat
      661 cctgcggtga ggcctgcacc ttgtctaccc agtcaaactt ttgctgggta agatttcgca
      721 ccttttgggc aacctcgaat ccggtgccgg ttgtcgtcgt ctgggcctcc gcgtacgatg
      781 tagctccgaa caaaaatacg aaactcacta cgcacgcaac aactcgataa aatactttgg
      841 tactcatatg tttttgttat gttacttata tcttttcggc ctgaatcata attcgcttat
      901 tatcaattcc agggggccag cagcttagca ataccaccct cgacacttcg ctctccataa
      961 gatcggaagg aatgtcttcg cccctatgga gaatggtttt ccctgctacc cggtactggt
     1021 gcgccactcc gtttatgctt acggaaagca tatccccttg ttccaatttc gttaagtcgg
     1081 aaaatacgcc gtcaaagtaa cttcccagcc accccggggg agccgaatgc cccaacaaaa
     1141 ctgcggtgcc cttctgcccg ggaattgcag aagggtaatg cgccactcct ttttccaagg
     1201 gctccagaaa gtcgtcggga ttgctgctgt ccacgaaata cacaggggca gccaccccaa
     1261 tttttggaat tgtaagggaa gcctccttga tttcaaacgg agccgttgtc gaaagggtgt
     1321 cgttttgcgg agcgttttct tctctggcgt aagagggagt aagatacgca tccactttct
     1381 gaaaagccca catcagccca aaactcgccg ccaaaaagag gtatacgaaa agggctcgcc
     1441 tgccgtaacg cgagatgcct tcctcgccca aataaagcag attttcttta attacgcgcg
     1501 tctccataat ggctgcctat cataaaaaaa gacggcggtc aagcgcccgc cgtctctcgt
     1561 aaagctcaag aatacttatt ttcctttctg aatgtctacg agttccacgt cgaatagaag
     1621 agtggcgttg ggcggaatta catccatgaa ccctctctcg ccatatgcca actcgggcgg
     1681 tatgaccaat tgttttttct cgcccacttt catgccctga attccctgat cccaacctgc
     1741 gatcacatcg cccgcaccca gcgtaaactg aataggcgcg ccctttgcgc gagagctgtc
     1801 gaacacggtt ccgtcttcga ggcggccctc gtaatgcacg gcaacagtat cgcctggttc
     1861 tgcttgttcc atagattctt gcggcttcaa gaaagaaccg taccatatga cggcccccgc
     1921 caaaaccacc gcggccaaag ctataataaa ttgcttcata aaggtttctt ttcgtcttct
     1981 ctgggacgaa taaccatttt ttgtatcagc ttaaaggcct cgtctgcgtt gtcgaccaaa
     2041 tggtacaact ctttgtcttc tttgcctatc gcttttcgct tttcgtacag ttccttttct
     2101 acccacttta aaagcggctt ccaataccct ttgtcgacta agataatggg aatgggttcg
     2161 attttccctg tctgcacaag cgtagccagt tcgaaaaatt cgtcgagtgt gccaaaacct
     2221 ccggggaaga aaacgtacac ttccgaagca aatgcaagca tcacttttcg aacaaagaaa
     2281 tagtaaaacg cctctgcctc ttttacgtat cggttggtgc gttgttcttg aggcagctga
     2341 atgttaatgc cggccgacct tccccctgct tcgtatgcgc ctttattcgc ggcctccata
     2401 atgccggggc ctcccccggt aataatggca aaaccgtctt ttgccagggt ataggccaac
     2461 ttctctgcct ccagatacgt tcggtgttct gcacccacgc ggccgcttcc aaaaaatgtt
     2521 gctgccagtt caaaatcgcg caggaagtga aaccctacca cgaactcttc caaaattttg
     2581 ctcatgcgct ctggctgcac ttcttccgga tgcattttaa gcaaggcttc aacgcgcggc
     2641 ccagtaaaac aaatttgatt ggatcgggaa acgttcatca tgcaggtttt tttgctcgtt
     2701 ggtctcttaa tgtttgcacc attgccccca agccaaatac tacagcgccc agagaaatcg
     2761 cccaccccac aatagggaaa aagctaaaca cagaatacag caccaagccg attaagaagg
     2821 ctaacatgcc gttttccttg ccgtcgaagt actgcaatat aatattgcca aaaagaatca
     2881 tcgcaaaaat cctcgcaacg tacagtacca aagaagaaac aagcaacagc acaagcccca
     2941 gaggaatacc cacaatagta aggatcaaaa gccccaccaa aagaggcagt agcagcaaca
     3001 cggtaactcc cgtcgtgaga gattttattt tcctggtttt tagggttgcc accgtgcctt
     3061 tggtgaaacc ggggaagaag tggagaaaaa tccaccccaa aatcaaagtg gttacgaagc
     3121 ttgtaagccc aaggaatggt ttgataaaag gctgtatcgg actcaatacc tctgtaatag
     3181 gtgccgcgag ctctgccggg ctttctttaa tggtattgcc ctcgattgta gcgccctgtg
     3241 ctatcaaagg ttcgcggttg ctccagtagg taaagtctcc cccgactttg gcattcgaag
     3301 taaagcgtat ggtatcgacc gcagcttcca agtcaccctc gatagcgtta tctaaggtaa
     3361 gagtgcgccc cgcgattctt gcttctttct gcacgggtgc agccaagtgc acgtttgcag
     3421 cggccaccac tattccgccc agaacttttg ccgagtcggc aaaagttacg ttcatgccgg
     3481 ccgctgtcac atttcggcct acttcaccgg cgattaccac ctggcctccc atggctcgta
     3541 cgttctgcgt aacttttccg gtaatggtaa tggtaccccc aacgactatt aaatcacctt
     3601 cgataacccc gtttacaaaa acctgggctc ccgccacata ggcatcccct tgaatagtac
     3661 ccgcaatttc taccgtttcg ccggcaacgc caaaatcttc ggtaattacc atgtccgagg
     3721 gcaccagctt tcttccttct tcgtcactgg agttctttgt cgcggcgagc aacggggaaa
     3781 acaacacccc aattgcgata atagctatac aaaacttttc tttcatacca agtccttttc
     3841 ctattattat acgccgttct caaccgtttg cacactcgtt aaattcgcgg gcttcgacat
     3901 tgtattcgcc gataagcgct tggacgtctt ccaccagctt attgtattgc gctaccaact
     3961 ggttatagtg gtccacccgg tctctgaaca cctggcctct tttgggttct gttgcttcga
     4021 tctctttgta ctctttgtcc aagatcgctt ccatctcttc gatttggatt ttctttagtt
     4081 caatatcgct ttccagttcg gagctcggca ttgaacaagc cgaggaaggc agcccgaata
     4141 tctgcaccgc tatccacacg gtatttcctt cgaattcgcc ctgctccaca gcaatgccga
     4201 tttcctggta ccgggggctt acgatatttt ccctgtgccc ggggctgtcc atccaggcct
     4261 gcaccaatgc gctgtctgtc gcaaagtttc ccaaagcaag gttttcgccg atcgcgagaa
     4321 acgcgtattc ctctttttgc gcggcctccg ccaccccaga gccgtcggga gctacgtggc
     4381 caaaatactg cttttcaaac atatccctcg cccgggccgc ggcaacgtta tctaaagtcg
     4441 tgtctgcctc cagcaaagaa agcccttgct cttggcgctg ggtattagtc cattgaaaaa
     4501 ccccctgctt ggttaagaaa gaggtagggg cttcttcgcc agaacgcaaa ggagctggcg
     4561 tggaaaccgc ttgcttgagt tcttctacca gcaaactaaa atctctgcgc tgggtaaata
     4621 ttccccggcc ttcagccgcg ttctcaaaca aaggcgacag tttggctatt ggcaaaacgt
     4681 cgccaaacac tcccaaaaac aaattcaaag caaaaagaat acacgcggcc gacacaatca
     4741 caacgacaaa agtagtcttc atatcaagac tattgtacct ttcagaaccc aaaagggcaa
     4801 accttattga atctcgtagc ggagggtgac gtttactgtt acgtcttgag agccgggttc
     4861 aactttggga gccacagagg gcgcagctcc accgattccc tgttcgagcg ccctgtcgag
     4921 ttcgtataaa ggatcgtaag gagatccgga ttcctgaatg gagagcaatc tccctactct
     4981 aaagccgccg gctttggcca ccgcctctgc ttttactttt gctttttcga ttgcctgggc
     5041 gcgcgccctg ctttctacgg cgtcgcggtc gtcaatggta aagaacaatt gcgacacgtt
     5101 attggctccg ttgtccacta cgcccgaaag caagactccc gctttctgca agtcgcgcac
     5161 ttttaccgac acggtttgag aaatggtgta tcccacgatt tctgccggag gacacgcttc
     5221 tggaaccaaa acgccttcac caataggaag cggccgaggc ttacaatcaa aatactgata
     5281 gcgcggattt acgttgtacc cttgcgtttt tatgtctttt gcctccacgc cctgtgactt
     5341 tacatattct atggctgcgt ttgttttttc tgaactttgg gtttgcaaag cctgcaggtt
     5401 tgttccccct tcggtaatta cgctaaacgt aaactcggca atatcgggaa ttgccacctc
     5461 tttgccttct gctgaaatct ggaaacttcg gaacgacgaa ggttctatag acttggaata
     5521 ggcatccacg tacacccata taacataggc gaaaaacacc aggcctaaga taactactac
     5581 tcctagtaca tcttttatgc gttcattcat catatgtgta aaaattactg ttatgcttat
     5641 cttat
//
LOCUS       DASRUP010000042         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1608574, whole genome shotgun sequence.
ACCESSION   DASRUP010000042 DASRUP010000000
VERSION     DASRUP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608574"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFE94_01705"
     CDS             <1..652
                     /locus_tag="VFE94_01705"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZX49850.1"
                     /translation="YSFSHEFLLPRYAVIDAELTYSLPPNVTADSGMDALAQAIEAFW
                     STNATPESKEYSRQAIQLILPNFVIAVNSPIPQAREAMAQGAHLAGKAINIAKTTACH
                     ALSYPFTVHYGVAHGHAVGLTLPEMIEYNAKTMEKADLQELVLLLGAKTPAQAANNIR
                     SLMQEISLETTLRELGITDLRQIQELVLKEVNTERLGNNPRLLAPDDIRAILAKIF"
     gene            complement(720..1283)
                     /locus_tag="VFE94_01710"
     CDS             complement(720..1283)
                     /locus_tag="VFE94_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZX49851.1"
                     /translation="MVTEKRATAVTTVFMVRNIGSGPEVAIQTRNIVTEGYDPEGKDK
                     IEVGPGETMRACEYPYDAALRAIQEEWGVPMPKDHVVHIYGGYERDEQNRRITLITPP
                     LPIMVNNEYDYPWVSVPVVLHVDGKLEFDEELGDGEAKEGRFMPVQELLHWLEKSPQD
                     FFRLTYAGAKELCRMIIDGKLEVPVHP"
     gene            complement(1285..1824)
                     /locus_tag="VFE94_01715"
     CDS             complement(1285..1824)
                     /locus_tag="VFE94_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HZX49852.1"
                     /translation="MFFIHTIDFNKKGDDIGPGTIARAKGALYKAVQRNETKRYFLVT
                     AGTPAPTTGEPMCLLVKRWLIKQNIPSDWILTPQIPRADNTAAEIVDAMQIVEQKFLH
                     GEPFFDCSSLWHIPRIAFMWPMYGRRVRPRFVWVLDRKLFSPRWLIREMLGIGALMLR
                     LTGPRWIPQELEPETEGRN"
     gene            1944..>2821
                     /locus_tag="VFE94_01720"
     CDS             1944..>2821
                     /locus_tag="VFE94_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49853.1"
                     /translation="MKNVAIRLVIFFVAVCVTLLVLEGVVRLLHAHTTLVSVPVQEPA
                     SFLQQNHELKYVPAPNFGPFPFRVEHIEYELVSNEFGCFEKPYNGEDPYVLLVGDSFS
                     HHLASLEEKWGSQLEKELGTRVLQCGVSAYSTTQELGFAKRIVSEIGKPPRLIVVGYF
                     LNDFAEEMRNRPASALKEAESPNILSIANAATDDTQKDTLPTLGGTNRFIQTTRWLHE
                     HSFLYRLFRIPLKSTLLNIPYLGDQLLDMGVLQKQATSFDQMLLYEQVAENAALQESW
                     QAHVESMRAFKQFANE"
BASE COUNT          655 a          695 c          717 g          754 t
ORIGIN      
        1 atattctttt tcgcacgaat ttttgcttcc gaggtacgct gttatagacg ccgagcttac
       61 gtattctttg ccgccaaatg ttacggcgga ttccggtatg gacgctttgg ctcaggccat
      121 agaggctttt tggagcacca acgcaactcc agagtcaaaa gagtattcca ggcaggcaat
      181 ccaactcatc ttgcccaatt ttgtcattgc agtaaacagc cctattccgc aggcaagaga
      241 agccatggca caaggggctc atttggcggg caaggcaatt aacattgcca aaaccacggc
      301 atgccacgca ttgtcttatc cttttaccgt gcactatggg gttgcacacg gccatgcggt
      361 aggtctcaca ttacctgaaa tgattgagta caacgccaaa accatggaaa aagctgactt
      421 gcaagaactg gttttgttgc ttggggcaaa aactccagcg caggccgcaa acaacattcg
      481 cagtttaatg caggaaatta gtttggaaac cacgctgcga gagctcggca taacagactt
      541 gcggcaaatt caggagttgg tgctcaaaga agtaaacaca gagcgtttgg gcaacaaccc
      601 ccggctcctt gccccagacg acatccgcgc catcctcgca aaaatatttt gaaataaaat
      661 tgggagtcct gtggggggct cccttttgtt gtgctatgtt gcgctcgcgt caactccttc
      721 tacggatgga cgggaacttc gagcttgccg tcgattatca tgcggcacag ctcttttgcc
      781 ccagcatatg tgagacggaa gaagtcctga gggctctttt ctagccagtg gagaagttct
      841 tgcactggca tgaaccttcc ttcctttgcc tcgccgtcgc cgagctcctc gtcgaactcc
      901 aacttcccgt ctacatggag gaccacgggc acagagaccc agggataatc atactcgtta
      961 tttaccataa tggggagtgg tggagttatc agagtaatgc gtctgttctg ttcatctctc
     1021 tcataaccgc cataaatgtg cacgacgtgg tctttgggca tcggcactcc ccattcttct
     1081 tggatagctc taagagctgc atcgtatggg tattcacagg ctcgcatggt ctcgcccggg
     1141 ccgacctcga tcttgtcctt tccttccggg tcgtaccctt cggtgactat gtttctcgtc
     1201 tggattgcga cttctgggcc agatccgatg tttcgaacca tgaataccgt tgttaccgct
     1261 gttgctctct tttctgttac catttcaatt cctcccttca gtttcgggtt cgagttcctg
     1321 gggtatccat cgggggccgg tgaggcggag cattaaagcc ccaatgccga gcatttcccg
     1381 aatgagccac ctggggctaa acagtttcct gtcgagcacc caaacgaacc gaggcctaac
     1441 tcttctgcca tacatgggcc acatgaaggc gatgcgcggt atatgccaca agctggagca
     1501 gtcgaagaat ggttcgccgt gcaaaaactt ttgttcgaca atttgcatgg cgtccacgat
     1561 ttccgcagct gtgttatctg ctcgcgggat ttgtggggta aggatccaat cagacgggat
     1621 gttttgtttg atgagccacc tctttacgag taggcacatc ggttcgcctg tggttggtgc
     1681 aggggttcct gcggttacca gaaagtatct tttcgtctcg ttgcgttgga ccgctttgta
     1741 aagcgcccct tttgctcggg cgatggtacc aggtccgatg tcgtctcctt tcttgttaaa
     1801 gtcaatcgtg tgtataaaga acactggcgt tgccaaaacg cacctccaag gttttgattt
     1861 tggtgggtgt agtatactat aaacggggga aatgtcaaga actgcggttt gcgtacagga
     1921 atgaagagag tataataatc tgaatgaaga acgttgcgat tcgcctagtt attttttttg
     1981 ttgccgtatg cgttacgctt ttggtgcttg agggggtagt gcgccttctg catgcccata
     2041 cgacccttgt ttcggttcct gttcaagaac cggcttcttt tttgcaacag aatcatgagt
     2101 taaagtatgt tcctgcgcca aatttcgggc cattcccgtt tcgcgtggaa cacattgaat
     2161 acgaacttgt ctccaacgaa ttcggctgtt tcgaaaaacc ttacaatggg gaagaccctt
     2221 acgttctgct ggtgggggac agctttagcc atcatttggc ttcgctggag gagaaatggg
     2281 ggtcgcagct cgaaaaagag ctgggcacgc gggtgctgca gtgcggcgtg tctgcctaca
     2341 gcacaaccca ggagctcggg tttgctaaac ggattgtgtc tgaaattgga aagccccccc
     2401 gactcattgt ggtaggatac ttcttaaatg actttgcaga agaaatgagg aaccgcccgg
     2461 cttctgcact caaggaagcc gaaagtccga acatattgag catagctaat gccgccacag
     2521 atgatacgca aaaagatact ctccctacct tgggtggaac caataggttc attcagacga
     2581 cacggtggtt gcacgaacat tcttttttgt accgactttt tagaattccc ttgaaaagca
     2641 cacttttgaa cattccttac cttggtgatc aacttctgga catgggagtt ttgcaaaagc
     2701 aggccacctc ttttgaccag atgctcttat acgaacaggt tgcggaaaac gcggccctcc
     2761 aggaaagttg gcaggctcat gtggagagca tgagggcttt caagcaattt gcgaatgaac
     2821 a
//
LOCUS       DASRUP010000043         6432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_956895, whole genome shotgun sequence.
ACCESSION   DASRUP010000043 DASRUP010000000
VERSION     DASRUP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956895"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1323)
                     /locus_tag="VFE94_01725"
     CDS             complement(115..1323)
                     /locus_tag="VFE94_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX49854.1"
                     /translation="MTKKTRLKKGAKPPKKAGAKKRKSSLLQKGSRKKQPIKRVVKKQ
                     PRKKRNAISDESIQELIEKSRARGFVTYSEILYTFPEIEKDIEGLEKLYQRLEELGIR
                     VEEVREYLEVDKPDQKDILSVEAEAPLDPVQMYLKEIGKIPFVTAEQERELAKRIELG
                     DEEAKKQLARANLRLVVSIAKKYVGRSPNLTLLDLIQEGNLGLFRAVEKFDWRRGYKF
                     STYATWWIRQAITRALADQARTIRIPVHMIETISRYTQVRRRLLQDLGREPLAEEIAA
                     EMGLEVDKVRHIQRISQETVSLETPVGEDDEDSVLAEFVEDEKVPPPSVDAARTLLRE
                     RLQEILDDLAPREQKILALRFGMEDGVTHTLEEVGAEFGVTRERIRQIEAKALEKIRE
                     HRSLKKLKGY"
     gene            complement(1320..3095)
                     /gene="dnaG"
                     /locus_tag="VFE94_01730"
     CDS             complement(1320..3095)
                     /gene="dnaG"
                     /locus_tag="VFE94_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZX49855.1"
                     /translation="MLSSPVEEIKNRLDIVEVIGGYIKLQKAGINYRAVCPFHSEKSP
                     SFFVSPARQIWHCFGGCSEGGDMFKFVMKIEGVEFADALRQLAQKAGIDLPRRDPQFA
                     KLQTEKQQLLELVELAAQFFEKQLAASGAGKEAKAYLAKRGVSQESMAKWRVGYAPDT
                     PHGLVQFLHQKGWQPEQMVRAGLAIQGAGSLYDRFQSRIMFPIFDLNSQIVGFGGRIF
                     GQKAQTEPAKYMNSPNTLIYDKSRILYGLDKAKVAIRQKNTCIVVEGYMDCIMASQAG
                     SANVVASSGTALTPMQLQILKRYCEQLHTAFDMDAAGDNATRRGVELALQQGFQVQVI
                     AMLHKDPADTILEDAVLWEKALAQTKSFLAHSFEVQLGKFDKTTADGKRKIAESLLPF
                     IIRIPNKIEQAHWVGYLAKELHIPEESVQEELKRVEAKEGVPERHREVPGHLDQARGK
                     KTRKEMLEERALSLMLAHSVLDLLNEDSIQYFSVRNQDILEGLRKQQGFFPDKAAEVF
                     EKEVVEQLQALALLAEVEDPPAGGEGEEWRGELEACFRELKMLYLKKRLDRLAEQIRD
                     AEGGKDGVRLQALLKEFHTIAREVR"
     gene            complement(3101..3226)
                     /locus_tag="VFE94_01735"
     CDS             complement(3101..3226)
                     /locus_tag="VFE94_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49856.1"
                     /translation="MVKAVIIVGVVLLLLFVGVIFFLEPGTINEGNQTENTMEQE"
     gene            complement(3237..4007)
                     /locus_tag="VFE94_01740"
     CDS             complement(3237..4007)
                     /locus_tag="VFE94_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49857.1"
                     /translation="MANQPPKRDYNAERKSGLRPGTSQWGRSMTQGLLMGFGGMVRSA
                     VSGPKAGRGQNATGKEYAILKRYGGVTEELDLEKALHFLQGSNMPLAAKKLAEAELRT
                     LGRANLTGNHITQEKIATAKEELEGIIGAPQTAEVIKTLEGMFDRGRERTGQEIEKRV
                     RGAKNLHPYQREVAARLLGGLDQKGQPLRKYGRISHQHIAQTAERLRSTMGEHAATLF
                     ERPFSPAEPKGMGGEAPGGSQRVGRLQEQREQGKETNQ"
     gene            complement(4037..4121)
                     /locus_tag="VFE94_01745"
     tRNA            complement(4037..4121)
                     /locus_tag="VFE94_01745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4085..4087),aa:Ser,seq:gga)
     gene            complement(4141..4674)
                     /locus_tag="VFE94_01750"
     CDS             complement(4141..4674)
                     /locus_tag="VFE94_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HZX49858.1"
                     /translation="MKKCPQCNVDFEQALVTNVQVDYCPRCYGLWFEESELDLAKDEK
                     DRSLRWLDIDLWKHEEKFQVSRGRELCPQDRMPLYEVRYGDSNVRVDVCNICNGIWLD
                     RGEFIEIIEYLREKGTREIMHSYAKNLAEEFWEVFAGPEMLRDEVLDFLTILKVLRYK
                     FVAQHPAVARLMLALPR"
     gene            complement(4696..5610)
                     /locus_tag="VFE94_01755"
     CDS             complement(4696..5610)
                     /locus_tag="VFE94_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZX49859.1"
                     /translation="MANLYTHAESNTRKTWIYLTGFFLFVVGVGWVMSYALGSPVILV
                     FAVVLSVIMSVGSYWYSDKIVLKMSGAKPIEKTDNPELYRLVENLSITAGLPMPKLYI
                     IQDAQPNAFATGRDAKHAVVAVTTGILERLERVELEGVLAHEMSHIGNKDMLISTVVV
                     VLVGIVVLLADFFFRMAFFGGLGGRNNNDRGGGQMHLIMIGVAVVFAILAPLAAQVIK
                     SAVSRKREFLADASGALLTRYPDGLARALEKISADPHQLKHANDATAHLYFASPFRGK
                     EQTSWIHKLFMTHPPVEQRVAALRGMQL"
     gene            complement(5661..6215)
                     /locus_tag="VFE94_01760"
     CDS             complement(5661..6215)
                     /locus_tag="VFE94_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZX49860.1"
                     /translation="MNPIYIILIVLGVIVAWVVLTYNRLINLRNRVKEAFSDIDVQLK
                     RRYDLIPNLVNTVKGYAVHESGVFQKVTEARTRAMGATGVHERAEAENMLTGALKTLF
                     AVSENYPDLKASTNFLELQRELRDTEDKVQAARRFYNNTVMSMNNKVETFPSNFIAQM
                     FSFKKEEFFELEEEAARSVPEVKF"
BASE COUNT         1436 a         1776 c         1413 g         1807 t
ORIGIN      
        1 tcccaaacgc tttgccaaag aagaaccgcc gtccaccagc aaatcagccc cttttaaaag
       61 aaggggcagt cccaataaaa aaccgagcaa cgcaaaaggc ataagtgcta actattagta
      121 ccccttgagt ttctttaagc tgcggtgttc gcggattttc tctaaagctt tcgcctcgat
      181 ttggcggatg cgctctctgg ttacgccaaa ttcggcgccc acttcttcta gcgtatgggt
      241 tacgccgtct tccatgccga atcgcaaggc gagaattttt tgctcgcggg gggccaggtc
      301 gtcgagaatc tcctgcaagc gctcacgcaa caaggtgcgc gccgcatcca ccgaaggcgg
      361 cggcactttt tcgtcttcca caaactcggc aagcacgctg tcttcgtcgt cttctcccac
      421 aggcgtttcc agagacacgg tttcttgcga aatgcgctga atgtggcgta ccttatccac
      481 ctccaagccc atttcggccg caatttcttc tgccaaaggc tctctgccca aatcctgcag
      541 caaacgcctt cgcacctgcg tgtagcggga aatggtctcg atcatgtgca cgggaatgcg
      601 aatggtcctg gcctggtcgg ccaaagccct tgtaatggcc tggcgaatcc accatgtggc
      661 ataggtggaa aatttgtatc ctcttctcca gtcgaatttt tctaccgcgc gaaacaaacc
      721 caaattcccc tcctgaatta aatcgagcag ggtaaggttt ggggaacgcc ccacatattt
      781 tttggcaata gaaaccacca gccgcaagtt ggcccgagcc aactgctttt ttgcttcttc
      841 gtcgcccagt tcaatgcgct tggcaagttc ccgttcctgc tcggcggtaa caaaagggat
      901 tttgccgatt tctttcagat acatctgcac cgggtctaag ggagcttccg cctctacgga
      961 gagtatgtct ttttggtccg gcttgtctac ctcaaggtat tctcgcacct cctccacccg
     1021 tatgcccagt tcttcaagcc tttggtagag cttttccaac ccctcaatgt ccttttcgat
     1081 ttcaggaaaa gtataaagaa tttcagaata cgttacaaag ccccgggcgc gcgatttctc
     1141 aatcagctct tgaatggatt cgtccgaaat tgcgttgcgc tttttccgcg gctgcttttt
     1201 aacgacgcgt tttatgggtt gttttttccg ggaacctttc tgcaaaagcg atgatttccg
     1261 tttttttgct ccggccttct ttggtggttt ggcgcctttt ttcaaacgtg ttttttttgt
     1321 catcgtactt ctctggcaat agtatgaaat tctttgagca gggcctggag cctgactccg
     1381 tcttttccgc cttccgcatc cctaatttgc tcggctaacc ggtcgagacg cttttttaag
     1441 taaagcattt taagctcgcg aaagcaagcc tcgagttctc cccgccattc ttctccttct
     1501 ccgccagccg gcggatcttc cacctctgcc agcagggcca aagcctgaag ctgttcgacc
     1561 acttctttct caaaaacctc tgcggccttg tccgggaaaa acccctgctg ctttcgcaag
     1621 ccttccaaaa tatcctggtt gcgcaccgaa aaatactgta ttgaatcttc gttcaatagg
     1681 tccagcacag agtgggccag cattaaagaa agtgcgcgtt cttcgagcat ttctttgcgg
     1741 gtttttttac cccgagcctg gtcgaggtgc ccaggaactt ctctgtggcg ttccggtacc
     1801 ccctcttttg cttcaacgcg cttgagttct tcttgcacgc tttcttcggg tatgtggagt
     1861 tcttttgcca aataccctac ccaatgcgcc tgctcaattt tgttgggaat gcggattata
     1921 aacggcagca gggattcggc tattttgcgc ttgccgtctg ccgtggtttt gtcgaacttt
     1981 cccaactgca cttcaaaaga atgtgctaaa aaggacttgg tttgggcaag cgctttttcc
     2041 cataacaccg catcctccaa aatggtgtct gctggatctt tatgcagcat ggcaattacc
     2101 tgcacctgaa agccctgttg caacgcaagt tctactccgc gccgggttgc gttgtctcct
     2161 gctgcgtcca tgtcgaaagc ggtatgcaac tgctcacaat agcgcttgag gatttgcagc
     2221 tgcataggag ttaacgcggt gcccgagctt gcaaccacat ttgcagaccc tgcttgcgag
     2281 gccataatgc agtccatgta tccttccacc acaatgcagg tatttttttg gcgaatggca
     2341 acttttgctt tatccagccc gtacagaatt ctgcttttgt cgtaaatgag ggtgttgggg
     2401 ctattcatgt atttggcggg ttcggtttgc gctttctgcc cgaaaattct acccccaaac
     2461 cccactattt gagagttcaa atcgaatata ggaaacataa tgcgcgactg aaagcgatcg
     2521 tacaaagaac cagccccttg aatcgcaagt cccgctcgca ccatttgctc tggctgccaa
     2581 cccttctggt gcaaaaactg tactaagcca tgaggggtat cgggcgcgta tcccacacgc
     2641 cattttgcca tactttcttg gctcactccg cgtttggcca aataggcttt tgcctctttg
     2701 ccagcaccgg aggctgcgag ctgcttttca aaaaactggg ctgccaactc cacgagttct
     2761 aaaagttgct gcttttctgt ttgcaacttt gcaaattggg ggtcgcggcg gggcaaatct
     2821 attcccgctt tttgcgccaa ctggcgcaag gcgtcggcaa actccacgcc ttcgattttc
     2881 attacaaatt tgaacatgtc tcctccttcc gagcagccgc caaaacaatg ccaaatctgg
     2941 cgtgcgggcg aaacaaaaaa agaagggctc ttttctgagt gaaaagggca cacggcgcga
     3001 tagtttattc cggctttctg gagttttata tatcctccga taacctccac aatatcaagc
     3061 cggtttttaa tctcctcgac tggagaagaa agcatatacg ttattcctgc tccatggtat
     3121 tttcggtttg gttgccttcg tttatggtgc ctggctcaag gaaaaaaata accccaacaa
     3181 aaagcaaaag aagaaccact cctacaataa taacggcttt taccataatg atgtaattac
     3241 tgattagtct cttttccctg ctctctttgc tcttgtagtc tccccacacg ttggcttccg
     3301 ccgggcgctt caccacccat tccttttggc tctgcggggg aaaatggcct ttcaaataac
     3361 gttgctgcgt gctctcccat tgtgctcctt aagcgttccg ccgtttgagc aatatgctgg
     3421 tgagatattc ttccatattt tcgaagaggc tgcccttttt ggtcaagccc tcccaaaagg
     3481 cgcgctgcaa cctctctttg gtaaggatga aggttttttg ctcctcgcac tcgcttttcg
     3541 atttcctgac cagttctttc tctccctcta tcaaacatcc cttccagtgt ctttattact
     3601 tctgcggttt gcggagcgcc aataattccc tccagttctt ctttggcagt cgcaattttt
     3661 tcctgtgtga tatggtttcc tgtaaggttt gctcttccca gggtgcgaag ttcagcttcc
     3721 gccaattttt ttgcggcaag aggcatgttt gacccctgca agaaatgcag cgccttttcg
     3781 agatctaatt cctcggtaac cccgccgtat cttttgagta ttgcatactc cttgccggtc
     3841 gcgttttgac cacggccagc ttttggcccg gacactgcgg atcttaccat acctccaaac
     3901 cccatgagta agccttgtgt catgcttctg ccccactggc tggttccggg ccgcagaccg
     3961 cttttccgtt ctgcgttata gtctcgtttg ggtggttgat ttgccataga agtttacact
     4021 gagcgtagtc gaagtgcggt gggggtggga tttgaaccca cgataccttt cggtatacct
     4081 gctctccaag caggcgcact aggccgctat gcgaccccac ctttattata ccctattctc
     4141 ctacctcggc aaagcaagca tcaggcgcgc aactgccggg tgctgcgcaa caaacttata
     4201 ccgcaaaacc ttcaggattg tcaaaaagtc gagtacttcg tcgcgcagca tctcgggccc
     4261 ggcaaatact tcccaaaact cttctgccag gttctttgca tagctatgca taatctcccg
     4321 cgttcctttc tcgcgcaggt attcgataat ttcaataaac tcgccccgat ccagccaaat
     4381 gccgttgcat atgttgcaca cgtcaactcg cacattcgaa tcgccgtagc gcacttcgta
     4441 caaaggcatg cggtcttgcg ggcaaagctc tctgcctcgg cttacctgaa acttctcttc
     4501 gtgcttccac aaatcaatgt cgagccagcg caaactccgg tctttctcgt cttttgccaa
     4561 atccagctcg ctttcttcaa accaaagccc gtagcatcgc gggcagtaat ctacttgcac
     4621 atttgtcacc aacgcttgtt caaaatctac attacattgc ggacactttt tcatatgtaa
     4681 attcgaaatt aaaaattaca gctgcatgcc gcgcaaggca gctacgcgct gctccacggg
     4741 cggatgtgtc ataaacaatt tatgaatcca gcttgtttgc tctttgccgc gaaacgggct
     4801 ggcaaaatac aagtgtgcgg tggcgtcgtt cgcgtgtttt aattggtggg gatctgcgga
     4861 aattttttcc aacgcccgcg caaggccatc tgggtatctg gtaagcaaag ctcccgacgc
     4921 gtccgccaaa aactcccgtt tgcgcgaaac cgcggatttt atgacttggg ccgccaaagg
     4981 ggccagaata gcaaacacaa ccgccacccc aatcataata agatgcattt gcccgccgcc
     5041 ccggtcgttg ttgtttctgc cgcccaaccc cccgaaaaac gccatgcgaa agaaaaagtc
     5101 agccagcagc accactatgc ccaccagcac caccaccacc gtggaaatta acatatcctt
     5161 gttcccaata tgcgacattt cgtgcgccag cacgccttct aactctactc tttctaaacg
     5221 ttccagaatg cctgttgtaa cggctaccac ggcgtgtttt gcgtctcttc ccgtggcaaa
     5281 cgcgttgggt tgggcatctt gtattatata caattttggc atgggcaggc cagcggtaat
     5341 ggaaaggttt tccaccaaac ggtacagctc gggattgtct gtcttttcaa tgggctttgc
     5401 cccgctcatt ttgagcacga ttttatcaga ataccaatac gaacccacgc tcataataac
     5461 gctcaacacc acggcaaaaa ccaaaattac cggactgccc agcgcgtaac tcataaccca
     5521 ccccactccg accacaaaca ggaaaaaccc ggtaagatat atccaggttt tccgggtatt
     5581 tgactcggcg tgggtatata aatttgccat agacaaaagg aggaagcttg gggcgaagag
     5641 aggcaaattt tgaaatcgtc ctagaacttc acctccggca ccgagcgcgc ggcttcttct
     5701 tcgagttcaa agaattcttc cttcttaaag ctaaacatct gcgcgataaa gtttgaagga
     5761 aacgtttcca ctttattgtt catgctcatt accgtgttgt tgtaaaacct tcgggcagcc
     5821 tgcaccttgt cttccgtatc gcgaagctcg cgctgcagct ccaaaaagtt ggtggaagct
     5881 tttaagtctg ggtaattttc cgaaaccgcg aaaagagttt ttaaggctcc cgtaagcatg
     5941 ttttctgctt ctgccctttc atgcactccc gtggctccca tggcccgcgt gcgggcttcg
     6001 gtaacttttt ggaacacccc gctttcatgc acggcgtatc cttttacggt attaacgaga
     6061 tttgggatta agtcgtacct gcgctttaac tgaacgtcta tgtcagaaaa cgcctctttt
     6121 acgcgattgc gcaggttaat taaccggttg taggtaagca ccacccacgc cacgataact
     6181 cccaggacta taagaataat gtaaatagga ttcatatgac tctattatag taccaaaaaa
     6241 gccccattgc aagagctttc aactttagcc tacgggacat actatttcag taattcattt
     6301 aatgagttta cggatctcaa gtttcccttt ctgtattctt gctcgccctt tgctattgct
     6361 tgcaacgcct gtgcttcttc ttcccaagcc tttttgaatt tggggttctt aagccgtttt
     6421 ttcaaatctt gt
//
LOCUS       DASRUP010000044         5603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_854933, whole genome shotgun sequence.
ACCESSION   DASRUP010000044 DASRUP010000000
VERSION     DASRUP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854933"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="VFE94_01765"
     CDS             <1..1863
                     /locus_tag="VFE94_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZX49861.1"
                     /translation="ELLLQKEDKYFFYHLGVNPWSTVQAALYQVGVGERKASSTITQQ
                     LVKVLLGNESNRTLQNKITEFLYTVALELFQSKETILTMYTNSVYFGNQTQGLFAASR
                     AYFGVDPELLSDSQIVQLLATINRPTSNNPSQPTNKPSSLLLASLLHVPATEDMFQGH
                     QETNTKAKTYFAKDHASFELLPFLPSLPASCQFTIDLELTRKVRKIVEQTIAQLKAKD
                     ARNAAVVVIKLPENELLAMVGSPDPDSFQDGYKINMALEPRPIGSTIKPFIYMKAFEK
                     ELRPYTLVDDREYKYITALGFPLYPQNFDSKYRGEVTLHYALSNSLNVPAVKVLEYVG
                     LENFYQFLERDLEFTPIQDFENYQLGIALGTLEMSLFDLAHYFTIFPNKGMLSPTTVF
                     RDNSCAKQEQNATEKRVGEEPYVQLLNKILNDRVTGIEQFGLVSELNLFQDNYALKTG
                     TSRDFQDSWIIGYTPDFLVGVWLGNADATSTDKLSGQIGAGKIWAQTMELLLHSPYND
                     KTPFDFSHVKGFQKTAYIEYGLLGDNYEYHRDILKAQDTSLIVEPHGGDVFLLQTNSH
                     IMLQAKKVVRWFVNGEPQGSGSSLSFVPSTPGEHTIQAIAEDHQEETITIDIVE"
     gene            complement(1869..>5603)
                     /locus_tag="VFE94_01770"
     CDS             complement(1869..>5603)
                     /locus_tag="VFE94_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZX49862.1"
                     /translation="VQVFNSEQDEIFNRKLPLNGFGTFDTKVFLEANAPLGSYRICAQ
                     EYACISVDVQQYVPAAFEVTLQSDKEEYISKDTVKLNVDANYYFGVPVEAGEVTYTVS
                     SQNYYFDRFSDEYFSFGTQSYYEYPFSYGDKFLLRGTTSLTAGKAEIQQLLDLGELLG
                     DEAGQGKLIIFDVTLQTADGKSVSAQKSFVAHPGELYLGVRADRSFVGKNQDIPLRVK
                     SVDTQGKEVGVGDAKMTIYRLEWVYAKRQGTGGGYSYTWERKKEQVQQYDFRTDGDGN
                     YSKTIQLAKEGQYEVEVSTTDRRGNAIQAATNVYVYGEGSVSVQPTQGISLELEPKET
                     NLSVGDTGEIVIKSPYERAKAFIAIERGKVFEYEIKDIRGNLSNFTFQVQEQYAPNVF
                     VSVLLQSPDPEIKFGQVEFRVGAERKELHIEVKADQTHYLPGEEVRLDIVAKDSLDQP
                     VETEVSLAVVDLSVLALKGNPKKNPLVFFYGGFPLTVTTSSNIKNVLVEAELSATKGG
                     GGGASQEDLATKARGDFREVAFWQARANTDRNGKATLTFTLPDNLTRWQAESVGVTKD
                     TKVGVGYSEFTTQKDVMLVPLRPRFVVPGDIFMVGAKVFNQTEQRQNLEVSFESETLV
                     SQDNKTRTNMSLDKGETTTLYFAVRAPESMEQGEHRFVLSAKNNAYEDTVIQTIPISA
                     NHTYEVTATANYTADEVSKEYVFLPDNIVREKGDLSVQQSATLAVFLSDALQYLVQYP
                     YGCSEQVSSKLNAIAIIKRGLNLPNLGDKFQLQNITYGDREYSPEELIEIGLAELMNN
                     QTPDGGFSLWRDGEPSFYATLPVVETLRNLSAAGVPINEDSLRRAAEYLYREVTSNNR
                     IYQNRNNVILAAYELFQLPAWSNNTVLRQRITTIASDDLFINDQISNGALAQLALILH
                     EGNFPDNLEQKIYNTLDNRIDIDARGTFLESNSNFLWYYYETPIKNTALYLKAKVARE
                     GDVAVIDKVIRWLLNSRAKDGAWGSTNNTITVIDAFTDFLQWKRETESDFTLELLVNG
                     LSKDRFRFQAETILDQRQTTIPLSELKVGSNNGMEFSKTSHNNLSNSYYYDAALKYFL
                     PAAQIPPRDEGMSIVRTLHQLDDQKNEDPLSKAKVGDVLRVHLQLTVPKSRNFVTVED
                     FIPAGFEIVNLDLATEQKSLRLQEAELTGRQLHVDHKELRHDRAFLYTESLGPGVYEF
                     DYFIRALVPGTFTHLPAVASEMYFPEVFGRTPGGNFQIEQ"
BASE COUNT         1370 a         1505 c         1271 g         1457 t
ORIGIN      
        1 gaacttctct tgcaaaaaga agacaaatac tttttttacc atttgggcgt aaacccctgg
       61 agtaccgtgc aagctgcgct ctaccaggtg ggcgttggcg aacgtaaagc ttccagcacc
      121 atcacccagc agctggtaaa agttcttctt ggcaacgagt ccaacagaac cctccaaaac
      181 aaaataaccg aatttctgta taccgtagcg ctggaacttt tccaaagcaa agaaacaatt
      241 cttaccatgt acacaaactc cgtgtatttt gggaaccaga cgcagggctt gtttgccgca
      301 agtcgcgcct actttggtgt tgatcctgaa cttctaagcg acagccaaat cgtccaactt
      361 cttgccacca tcaatcgccc tacaagcaac aaccccagcc agcccaccaa caagccctct
      421 tcgttattgc ttgcaagtct gctccacgta cccgcaacag aagacatgtt tcagggccat
      481 caggaaacca acacaaaggc aaaaacgtat ttcgcaaaag accacgcctc ctttgagctt
      541 cttcctttcc ttccctctct gcctgcctct tgccagttta ccatcgatct tgagttgacg
      601 agaaaagtac gaaagattgt ggaacagacc attgcccagc tcaaagcgaa agatgcaaga
      661 aacgcggccg ttgtcgtaat caagctgcct gaaaacgaac ttcttgccat ggtgggatct
      721 cccgaccccg actccttcca agatggctat aaaatcaata tggccttaga accccgcccc
      781 atcgggtcca ccataaaacc gtttatctat atgaaagcat ttgaaaaaga gttgcgccct
      841 tatacgttgg ttgatgaccg cgagtacaaa tacattactg ccctcggctt tcctctctac
      901 ccccaaaact tcgactccaa ataccggggc gaggttacgc tccactacgc cttgagcaac
      961 agcttgaatg ttcccgccgt aaaagtgttg gagtatgtgg gtctggaaaa cttttaccag
     1021 ttcctggagc gcgacctcga gttcacgcct attcaagact tcgagaatta tcagctgggg
     1081 attgccttgg gaactttaga gatgagcctg tttgacctgg ctcattactt taccatcttc
     1141 cccaacaaag gaatgctttc cccaacaacc gtgtttcgcg ataattcctg cgcgaaacag
     1201 gaacagaatg caacagaaaa gcgcgtagga gaagaaccat acgtgcaact cctcaacaag
     1261 attctgaacg accgggtgac tggcattgaa cagtttggcc tggtctctga actcaacctt
     1321 tttcaggaca actatgcctt aaaaaccggg acctcacgcg actttcagga cagttggatt
     1381 attggctaca cgccagactt tttggtagga gtgtggcttg gcaacgccga tgcgacttct
     1441 accgacaaac tgtcgggcca gataggggcg gggaagatat gggctcaaac catggaactt
     1501 cttttgcact cgccctacaa cgacaaaaca ccttttgact tttcccacgt gaaaggattc
     1561 caaaaaacag cctatattga gtacgggctt ttaggtgaca actacgaata ccatcgcgac
     1621 atcctgaagg cgcaggatac ttctcttatt gtggagccgc acggaggcga tgtgttctta
     1681 ctccagacaa acagccatat aatgctccag gcaaaaaagg ttgtccgctg gtttgtaaac
     1741 ggagaaccgc aaggctcggg aagctctctt tcgtttgtgc cttcaacacc gggcgaacat
     1801 accattcagg ccattgcaga agatcatcag gaagaaacta tcactattga tattgttgag
     1861 taataccctt attgctcaat ttggaaattg ccgcctggcg tccttccaaa tacctcggga
     1921 aagtacattt cagaggccac tgccggcagg tgcgtaaacg tgccgggaac caaagcccga
     1981 ataaagtaat cgaattcgta tactccgggg cccaggcttt cagtgtagag aaatgcccga
     2041 tcgtgccgaa gctccttatg atctacatgc agctgcctac cggtaagctc tgcttcttgc
     2101 aggcggagcg atttttgctc ggtggcaagg tcgagattta cgatctcaaa gccggccgga
     2161 ataaaatctt cgacggttac gaagttgcgg gacttgggta ccgtgagctg caaatgtacg
     2221 cgcaacacgt cccctacctt tgctttggag agcggatctt cgttcttttg gtcgtcgagc
     2281 tggtgtagcg tacgcacaat gctcatgcct tcgtctcgag gcggaatttg cgcagctggc
     2341 aggaaatact tgagcgcagc gtcgtaatag tagctgttag aaaggttgtt atggcttgtt
     2401 ttggagaact ccataccgtt attactgcct accttgagtt ctgaaagagg aatggtggtt
     2461 tgccgctggt ctaaaatcgt ttctgcctgg aagcggaagc ggtctttcga cagcccgttc
     2521 accagaagct ccaacgtaaa gtcagattcg gtttcccgct tccattggag aaaatcggta
     2581 aacgcgtcga ttacggttat ggtgttgttg gtactgcccc aagccccgtc ttttgccctt
     2641 gaattcaaca gccaacgaat taccttatcg ataaccgcaa catccccctc gcgcgccact
     2701 tttgctttca ggtacaaggc ggtgttttta ataggtgttt cgtaataata ccaaagaaag
     2761 ttgctgttag actctaaaaa cgtgccccgg gcgtcaatgt ctatgcggtt atcaagggtg
     2821 ttgtatatct tttgttccaa gttgtcgggg aaattccctt catggagaat gagggcaagc
     2881 tgggccagcg cgccattgct tatctggtcg ttgataaaca agtcgtcaga agcaatggtg
     2941 gttattcgct ggcgcaatac ggtattgttg ctccaagctg gcaattggaa taattcatat
     3001 gctgccaaaa tcacattatt cctgttttga taaatgcggt tattactggt tacttcccgg
     3061 tacaggtact cggctgcccg gcgcaggctg tcttcgttta tgggaacccc ggcagcagac
     3121 aggttgcgca atgtttctac aaccggaagc gtggcataaa aactcggctc tccgtctctc
     3181 cataaggaaa accctccgtc tggcgtttga ttattcatga gctctgccag cccaatctca
     3241 attaactcct ctggcgaata ctcccgatct ccataggtaa tattttgaag ttggaactta
     3301 tctccaaggt tgggaagatt caacccccgc ttgataatgg caatggcatt taacttgctc
     3361 gaaacttgtt ctgaacaccc atagggatac tgtactaaat actggagcgc gtccgataag
     3421 aacacggcaa gcgttgccga ttgttgaacc gacaaatctc ccttttctcg cacaatattg
     3481 tccggcaaga atacatattc ctttgatact tcgtctgccg tatagtttgc agtcgccgtc
     3541 acttcgtagg tatgattcgc gctaatagga atagtctgga ttaccgtatc ttcataggcg
     3601 ttgttttttg ccgaaagaac gaaccggtgt tcgccttgtt ccatggattc tggagctcgc
     3661 acggcaaaat acagggtcgt ggtttccccc ttatcgaggc tcatgttagt ccttgttttg
     3721 ttgtcttgtg aaaccaaagt ttcgctctca aacgaaacct cgagattctg cctttgctcg
     3781 gtttggttga atacttttgc tcccaccata aagatatcgc ccggaaccac aaatcggggc
     3841 cgcaagggca caagcataac atctttttga gtggtaaatt cgctgtaccc tacgcccacc
     3901 ttcgtatctt tcgttacccc taccgattct gcctgccagc gcgtaagatt atctggcagc
     3961 gtaaaggtaa gggtggcttt tccgttccgg tcggtgtttg ctcttgcctg ccaaaacgca
     4021 acctcgcgaa agtccccccg cgccttggtt gccaagtctt cctggctggc accgccgcct
     4081 ccccctttgg tagcagagag ttcagcttcc acgagcacat tcttgatatt ggaagacgta
     4141 gtaaccgtaa gcggaaaccc gccgtagaaa aacaccaacg gatttttctt cgggtttccc
     4201 ttcagggcga gcacgctcaa atccaccacc gcaagagaaa cctcggtttc gacaggctga
     4261 tccaaagagt ctttagccac aatatcgagc cgcacttctt cacccggcag gtaatgggtt
     4321 tggtcggcct tcacctcgat atgcagttct ttgcgttccg cccccacccg gaactccacc
     4381 tgcccgaatt taatttcggg gtctggcgat tggagcaaca ccgacacgaa aacattcgga
     4441 gcatattgct cttgtacctg gaatgtgaaa ttcgaaagat tcccccgaat atcctttatt
     4501 tcatattcaa atactttgcc gcgttcaatt gcgataaacg cctttgcccg ttcatacgga
     4561 gacttgataa caatttctcc ggtgtctccc accgaaaggt tcgtttcttt gggctccaac
     4621 tccaggctta tgccttgggt gggttgcaca ctcaccgacc cttcgccata cacatacaca
     4681 ttcgtggccg cttgaatggc gttgccgcgc cggtctgtgg tagaaacttc cacttcgtac
     4741 tggccctctt ttgccagctg aatggttttc gagtaatttc catctccatc ggtacgaaaa
     4801 tcgtattgct gcacctgctc ctttttcctc tcccaggtat aggaataccc tccgcccgtt
     4861 ccctgccttt tggcatatac ccactcaagg cggtatatag tcattttcgc gtcccccact
     4921 cccacttcct ttccctgggt gtccaccgat ttcacccgca gagggatgtc ctggttcttc
     4981 cccacgaacg agcggtctgc acgcacgcca aggtacagtt cgccgggatg cgccacaaac
     5041 gacttttgcg cggaaacact cttgccgtcg gctgtctgca aggtaacgtc aaaaataatc
     5101 aatttcccct ggccagcttc atctcccaac aattctccca aatcgaggag ctgttgaatt
     5161 tcggccttcc cggctgtcag gcttgtggtg ccccgcagca gaaacttatc gccgtaggaa
     5221 aagggatatt catagtagct ctgcgtcccg aatgaaaagt actcgtccga aaaacggtcg
     5281 aaatagtagt tctggctgct taccgtatag gttacttcgc ctgcctctac gggcacccca
     5341 aagtaatagt tggcgtccac gttaagcttc accgtatctt tggaaatata ctcttccttg
     5401 tcggattgca acgtaacttc aaacgcagcc ggcacatatt gctgcacatc aactgaaatg
     5461 cacgcgtact cttgcgcgca aatccgatag gagccgagcg gggcattggc ctcgagaaac
     5521 actttggtat cgaatgtccc aaagccgttc aacggcagtt tcctattaaa gatctcgtcc
     5581 tgctcggaat tgaacacctg cac
//
LOCUS       DASRUP010000045         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2256555, whole genome shotgun sequence.
ACCESSION   DASRUP010000045 DASRUP010000000
VERSION     DASRUP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256555"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2501)
                     /gene="rpoB"
                     /locus_tag="VFE94_01775"
     CDS             complement(<1..2501)
                     /gene="rpoB"
                     /locus_tag="VFE94_01775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZX49863.1"
                     /translation="MPLSSALPVKNFGKSSINLDLPYLLEVQHESWGIFWRQQLQELL
                     QEVSPIRDYTKKEFELWFLDYKLGEPNYPTELDAKLNDDSFEAPLRVKVRLVNLKTKE
                     VKEQEVFLCDFPLMTERGTFVLNGVERVAISQLIRSPGAFFTAQTGQGRKLFGAKIIP
                     NRGAWLEFETESSGFIAVRIDRKRKAAATTLLRAFGLETNEEILKLFSDVDTGETKFI
                     EETLKKDNSKNQGEGLVEVYRRLRPGDYVTPDTAKELIWNMFFNFERYDLSRVGRWKT
                     LQRLPALLKAKSGDEEVTIEDRVLKLEDVVEVLKEIIRLNNTPGAEPDQIDHLGNRRV
                     RTFVEFLQNRVRVGFMRMERIVKDRMSTLDPATITPSQLINPRPVMAVVKEFFTSSQI
                     TQFMDNENPLAELEHKRRVSATGPGGLTRERAGFEVRDVQPSHYGRICPIQTPEGPNI
                     GLVGHLAQYAKVNEYGFLETPYYKVEKGKVTKDLRYLTAYEEEQYVIAHAGVQLDPQG
                     RILSERVEARIKGEPGVADRDKVDFVDVSPQQFISIATALIPFLRNDDANRALMGSNM
                     QRQAVPLVKPQAPLVGTGYEERVAKDSGLAQVATEDGIVTEVDGRHIVVRSSSVSGKQ
                     ETKKNREYKLKNFVRTNQYTCFHQKPIVQKGDKVKKGQIIADGGAIDHGKLALGTNLL
                     VAFLPWRGGNYEDAIILSERVARDDVYTSIHLEDFTCDVRETKLGPEVTTSDIPNVGE
                     EKLKDLDEEGVVRVGAEVGPNDILVGKISPKGEADLTPEERLLRAIFGEKARDVKDTS
                     LLMPHGKRGRVVNVKVFSRDEGHKLEPGVMKRIHV"
     gene            complement(2638..3696)
                     /locus_tag="VFE94_01780"
     CDS             complement(2638..3696)
                     /locus_tag="VFE94_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZX49864.1"
                     /translation="MKLFLFAVLFLGGILAGSQVAFAQNASLYFVPSTGTVVPGQTLT
                     VQVMVNTGGAQVNAVGAYFTYPDTLLEGLSIDTTGSVLDTFTPEKTVGGGEVRFAAGK
                     LPPPFSGIHKLFSVTFRARSSLGTANLAFTGDSAILTDGDSRNIFSSATSGIGVFQVS
                     QSAAPPPSTPTPSPTPTQPGPTPAPGVDPVPSPPPAGGPAPPPIGAGLLFLANIDAQT
                     SDEGVLVSWDTSLPANAQVRYGETQNTEYHSSVVDAEYRSDHSILLSGVKLSDDYAFQ
                     VIGIDEAGFRVESQRLLFSDFREDKPSGFQIEDGQIAIGIGGLNLDLLRLLLFVALPI
                     VLVLIVGFLLFQRFRARA"
     gene            complement(3712..>4368)
                     /locus_tag="VFE94_01785"
     CDS             complement(3712..>4368)
                     /locus_tag="VFE94_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HZX49865.1"
                     /translation="FAASKAWSLSGGDGTKTVSYQCRDNSLNQNIATVSDTIVFDTTA
                     PSVSGLSPSGQLAAGTSQTEISLSTGEAATCRYSTTAGTSYDSMTNTFTTTGGTSHST
                     TVTGLSNGNSYSHYVRCADSLDNKNTTDSQVAFSVSSPPSPSPSPSPGGSSPSPSPSP
                     SPSASPIKGDCNADSKVNIFDLSVLLSNWGKTTTACDLNSDGKVSIFDLSVMLSNWTN
                     "
BASE COUNT         1032 a         1135 c         1096 g         1105 t
ORIGIN      
        1 tccacgtgaa tgcgcttcat tacgccgggt tccaacttat ggccttcatc tctcgaaaac
       61 accttcacgt ttacgacgcg cccgcgtttc ccatggggca ttaacaaaga agtatctttt
      121 acgtcgcgcg ccttttcgcc aaaaatggcc cggagcaaac gttcttccgg cgttaagtca
      181 gcctctcctt tgggcgaaat ttttcccacc aaaatgtcgt tggggccgac ttcagctcct
      241 acgcgcacta ctccctcttc gtctaaatcc ttgagtttct cttctcctac attgggaata
      301 tcagaagtgg taacttccgg ccccaatttt gtttcgcgaa cgtcgcaggt aaaatcttcc
      361 aaatgaatag aagtgtacac atcgtctctt gccacccttt ctgaaagaat aatggcgtct
      421 tcgtagtttc ctccccgcca aggcaaaaag gccacaagca agtttgttcc gagagccaat
      481 ttgccgtggt caatggcgcc gccgtctgca ataatctgcc ctttctttac tttgtcgccc
      541 ttttgcacaa tgggcttttg gtggaaacac gtgtactggt tggtgcgcac aaaattcttg
      601 agtttgtact cgcggttttt cttcgtctcc tgtttgccag aaaccgagga cgaacgcacc
      661 acaatgtgcc tcccgtctac ttcggttaca atgccgtctt ctgtcgcaac ctgggctaat
      721 ccggagtctt ttgccacgcg ctcttcgtat ccggtgccca ccagcggcgc ctggggtttc
      781 acaagcggca ctgcttgtct ctgcatgttg gaacccataa gggcgcggtt ggcgtcatcg
      841 ttgcgcaaaa agggaatgag cgccgtggct atggaaataa actgctgggg cgacacgtcg
      901 acaaagtcca ctttgtcgcg gtctgccacc cccggctctc cttttatgcg cgcctctact
      961 ctctctgaaa gaatcctgcc ctgcgggtct agttgaacgc ccgcatgcgc aataacatat
     1021 tgctcctctt cgtatgccgt aaggtaacga aggtctttgg taacttttcc tttttctact
     1081 ttataatacg gagtttccaa aaagccgtat tcattcactt tggcgtattg cgccaagtgg
     1141 cccacaagcc cgatgtttgg gccttccggc gtttgaatag ggcaaattct tccgtagtgg
     1201 gaaggctgca cgtcgcgcac ttcgaatccc gcccgctctc tggtgagtcc tcccgggcct
     1261 gtggccgaaa cacggcgttt gtgctccaat tccgccagag gattttcgtt gtccataaac
     1321 tgggtaatct gggaagacgt aaagaattcc tttaccaccg ccattaccgg gcgcggattg
     1381 ataagctgcg agggtgtgat ggttgcggga tccaaagtag acatgcggtc tttcacgata
     1441 cgctccatgc gcataaagcc cacgcgcacc cggttttgca aaaattccac aaacgtgcgc
     1501 actcttcggt tccccaggtg gtcgatttgg tcgggttctg ctcccggcgt attgttgaga
     1561 cggataattt cttttaatac ctccactacg tcttcgagtt ttagtacgcg gtcttcaatg
     1621 gtaacttctt cgtcgcctga tttcgctttc agtaaagcgg gcaggcgctg gagtgttttc
     1681 caacgcccta ctctggacaa atcgtaccgc tcgaagttaa aaaacatgtt ccagataagc
     1741 tctttcgccg tatctggggt cacgtagtcg ccgggccgca gccttctata tacttcaact
     1801 aaaccttctc cctggttttt gctgttgtct tttttaagcg tttcttcgat aaatttggtt
     1861 tctcccgtat ccacatcgga aaaaagttta agaatctctt cgttggtttc gaggccaaag
     1921 gcacgcaaaa gcgtggttgc ggcggccttt ctttttcggt ctatgcgcac ggcaataaag
     1981 ccggagctct cggtctcaaa ctccagccat gccccgcggt tgggaataat ctttgcccca
     2041 aagagctttc tgccctggcc cgtctgtgcg gtaaagaaag ctcccggcga acggataagc
     2101 tgggaaatgg caacacgctc cactccgttc aaaacaaaag ttccccgttc ggtcatcagg
     2161 gggaagtcgc aaagaaacac ttcctgctct tttacttctt tggttttgag gttcaccagg
     2221 cggactttca cgcggagcgg cgcctcgaaa gagtcatcgt ttagcttggc gtccagctcc
     2281 gtgggatagt ttggttcgcc cagcttgtag tctaggaacc agagttcgaa ttcttttttc
     2341 gtgtagtcgc gtatgggaga aacttcctgc aggagttcct gcagctgttg ccgccaaaaa
     2401 ataccccagc tttcgtgctg aacttccaat aaataaggca aatcaaggtt aatgctggac
     2461 ttgccaaaat tttttacagg gagtgcggaa gagagaggca tggacgtgca acaaaagata
     2521 cccttacttc tagcttaagg gcgccctcgg gcactaaaat tttttataga aagtcgaggg
     2581 gatcaattga atataaaaaa acccacgttt caagtatacc ttatcttata aaaaaggtca
     2641 agcccttgcg cggaagcgct gaaaaagcag aaaacccact atcaaaacca aaactatcgg
     2701 aagcgccacg aaaaggagaa gacgcaaaag atccagattt aatcctccaa tcccaatagc
     2761 aatctggccg tcctcaatct gaaatccaga gggcttatct tcgcggaaat ccgaaaaaag
     2821 cagccgctgg ctctctaccc ggaaccccgc ctcgtcaatt cctataacct ggaaagcgta
     2881 gtcgtctgaa agctttacgc cagaaagaag aatggaatgg tcgcttcggt actctgcgtc
     2941 gaccaccgaa gaatggtact ctgtattttg agtttctccg tagcgaactt gcgcgttcgc
     3001 cggcaaactg gtgtcccaag aaaccaacac accttcatcg cttgtttgag cgtctatgtt
     3061 tgccaagaac agcagtccgg ctcctatagg aggaggcgct ggcccgccag ctggcggagg
     3121 cgaagggacg ggatcgactc ccggcgcagg cgttgggcca ggctgggttg gtgttggaga
     3181 gggagtgggg gttgaaggtg gcggagcggc agattgacta acttgaaata ccccgatccc
     3241 ggaggttgca gagctaaaaa tattcctgct gtccccatcg gtaagtatgg cggaatctcc
     3301 ggtaaaagcc aggtttgcgg ttcccaaaga acttctggcg cggaatgtta cggaaaaaag
     3361 cttatgaatt cccgaaaagg gcggcggcag tttgccggcc gcaaaccgaa cttctccgcc
     3421 tcccactgtt ttttccggcg taaaggtgtc gagaacggaa cctgtggtat ctatagaaag
     3481 accttccagt aaagtatctg gatacgtaaa atacgccccc accgcattta cctgggctcc
     3541 tcccgtattt accataacct gaaccgtcag agtttggccc ggaacgactg ttccggttga
     3601 gggaacaaag tagagtgacg cattctgcgc aaaagccacc tggctacctg ccagaattcc
     3661 gccaagaaac aacacagcaa ataggaatag tttcatgagg atactatagc actagtttgt
     3721 ccaatttgac aacatgacgc ttaagtcaaa aatactaacc ttgccatcgc tgtttaaatc
     3781 acaggcagtc gtggttttcc cccagttgga aagcaaaacg cttaggtcaa agatgtttac
     3841 tttgctgtct gcgttgcaat ctccttttat gggagaagcg gaaggtgatg gcgacggaga
     3901 tggagaaggc gaactgcctc caggagaagg ggaaggggaa ggcgatggag gagaactcac
     3961 actaaaagcg acttggctgt cggtcgtgtt cttattgtct aggctatcgg cacatcggac
     4021 gtaatgcgaa taactgttgc cgttgctcaa accggtcacc gtagtggaat gcgacgttcc
     4081 gcctgttgtc gtaaacgtat tggtcatgga gtcatagctg gtgccggcag tggtggaata
     4141 tcggcaggtg gctgcctcac ccgtgctcaa agagatctcg gtctggcttg tgcctgccgc
     4201 aagctggcct gaagggctta aaccagaaac cgaaggagca gtggtgtcaa acacaatggt
     4261 atctgaaacc gtggcgatat tttggttgag gctgttatcc cggcactgat aagaaaccgt
     4321 cttggtgccg tctcccccag aaaggctcca cgccttgctc gcggcaaa
//
LOCUS       DASRUP010000046         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1296965, whole genome shotgun sequence.
ACCESSION   DASRUP010000046 DASRUP010000000
VERSION     DASRUP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296965"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFE94_01790"
     CDS             <1..262
                     /locus_tag="VFE94_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49866.1"
                     /translation="LKLQKASLHDRFDYAKKLADNAEQIPETLQNWLALTREMLHAAL
                     KENAKAALPLLSPVKTVQETLYLLRTTNVNPRLALERLMLNL"
     gene            374..1096
                     /locus_tag="VFE94_01795"
     CDS             374..1096
                     /locus_tag="VFE94_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49867.1"
                     /translation="MEAIRKVLHKLSGRGKATGTPIRISDATTEEKRIIEILGRVKVV
                     TLAEAADVWKQEVPEAPAIPFSEAGLRQCAEENNDGEDWRLVYILGLSLKAQRKIQGV
                     SSRDVVSGLRFAQGGYWRNPKDDYWTTQPKESGYRLLNMKPLKAPHGGWTWAEQQEII
                     DDLGPQYERADETIVSETVFSLGKSKDSVFCLNHWGKTVNSLDRHVVLEFNIFHGMSI
                     WGMAKSDERYRFGAVVERRADL"
     gene            1173..1730
                     /gene="frr"
                     /locus_tag="VFE94_01800"
     CDS             1173..1730
                     /gene="frr"
                     /locus_tag="VFE94_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZX49868.1"
                     /translation="MYEEIMQKVRPEMEKAVAFFQGELGKLRTGQASPALVEDVMVNV
                     FGQKMPIKQLAGISTPERRQLLIQPWDHSYVEPIEKALSQSSLGTSPIVEGNSIRVHL
                     PQLTQEYRQQLGKVLGEKAEDAKKTVRKAREDAWENVQENAKKGEIREDDKFRAKDEL
                     QKLIDEYNKKVDELVEQKKKEIEVE"
     gene            1733..2800
                     /locus_tag="VFE94_01805"
     CDS             1733..2800
                     /locus_tag="VFE94_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HZX49869.1"
                     /translation="MTIAIAIFTIGILVILHELGHFFAAKKFGVKVEEFGIGLPPRLW
                     GKQIGETIYSVNALPIGGFVRMEGEEKRSESPRSFNKKPVWQRLVIVGGGVFVFWVIA
                     AAIFAGLGATVGIPMAIDDNAQGVADPRVQIIGIASNSPAAEAGIVLGDTILRIQGQE
                     ITKMQEVRGIATTHAGQEITLEVRRGTETLQINLTPRIDPPEGEGAIGVAFVRTGMVH
                     YAWWQAPFRGIAHTAQLTVAILRTFADLISGLVAGQGLPPGTQFSGPIGVVGFLSDSL
                     GLGVSSFLSFVALISVYLAIFNTLPIPALDGGRMFFLLVEAVRRRPLPEKIEQRLIIT
                     SFIILIALIIWISIGDVRNRF"
     gene            2809..>3180
                     /locus_tag="VFE94_01810"
     CDS             2809..>3180
                     /locus_tag="VFE94_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase"
                     /protein_id="HZX49870.1"
                     /translation="MRQSELFTKTQKTLPKDEESKNAKLLLQAGFIHKEMAGVYSFLP
                     LGRRVMENIMQIIREEMNALDGQEIFLTSLQDSAPWRASGRWSDEVVDVWFKTKLKND
                     TEIGLANTHEEPLTALMRNHIQ"
BASE COUNT          857 a          729 c          836 g          758 t
ORIGIN      
        1 tctcaagctt caaaaggcct ctctgcacga ccgctttgac tacgcaaaaa aactggcgga
       61 caacgcagaa caaattcccg aaaccctgca gaattggttg gcgctcacgc gggagatgtt
      121 gcacgcagcg ctcaaagaaa acgcaaaggc ggccttgccg cttctttcac ccgtcaaaac
      181 cgtgcaggaa accctttatc ttttgcgcac caccaacgta aacccccgcc tggctttaga
      241 acgccttatg cttaatcttt aatcaaaaac cttgcttttg gcgtacccct tttaatttga
      301 ctttttgcaa aatacgtgct attatgctat ttagcagcaa ggtcccacag acttttttct
      361 ctaggagtct aatatggaag caatacgaaa ggtccttcac aagttgtccg ggagaggaaa
      421 agcaacggga actccaatcc gtatcagtga tgctactacc gaagaaaaac ggattattga
      481 gattcttggc agggtaaagg tggtgactct agccgaggca gcagatgtat ggaaacagga
      541 ggttccagaa gctccagcaa ttccattttc agaagctggc ttgcgccaat gcgcagagga
      601 aaacaatgac ggggaagatt ggcgacttgt gtatatctta ggcctctcct taaaagcaca
      661 aagaaaaatt cagggcgtaa gcagtagaga tgtcgtaagt ggacttcgtt ttgcgcaggg
      721 agggtactgg cgtaatccga aagacgacta ctggactacc cagcccaaag agtctgggta
      781 tcgcctgctc aatatgaagc ccttgaaagc accacatggc gggtggactt gggcagaaca
      841 acaggaaata atcgatgacc tgggtccgca gtacgaacgg gcagatgaaa caattgtttc
      901 agaaactgtc ttctcgctcg gaaagagtaa ggatagtgtt ttttgcctta accattgggg
      961 caagacggtc aattctctcg atcgccacgt cgtgcttgag tttaatattt tccacggcat
     1021 gtcaatttgg ggaatggcca aatccgatga gcgctatagg tttggtgcgg tcgttgagcg
     1081 tcgagccgat ttgtaaaagg gtatgcactt tttaagtaca ccctttttat tttgcgctcg
     1141 acaggataaa gactttttgg tacactgttg atatgtatga agagattatg caaaaggtga
     1201 ggccggagat ggaaaaggct gttgcgtttt ttcaggggga gctgggaaaa ttgcgcaccg
     1261 gacaggcaag cccagcttta gtggaagacg taatggtaaa tgtctttggc cagaaaatgc
     1321 ccataaaaca gctggccggc atttctactc cagagcgcag gcagcttctt attcagcctt
     1381 gggatcattc gtacgtagag cccattgaaa aagcgctttc gcagtcgtct ttgggcacaa
     1441 gccctattgt agagggcaac tccatacgcg tgcatttgcc ccagctcacg caggaatacc
     1501 gccagcaact gggaaaagtg ctcggtgaaa aagcggaaga cgcaaagaaa acagttcgga
     1561 aagccaggga agatgcgtgg gaaaatgtcc aagagaacgc gaaaaaaggc gagatccgcg
     1621 aagacgataa attccgcgcc aaagacgagc tgcaaaaatt aatcgacgaa tacaataaaa
     1681 aggtagacga gctggtcgaa caaaagaaga aagaaataga ggttgagtaa tcatgacaat
     1741 cgctattgca atttttacca taggaattct ggttatcctc cacgagctgg ggcatttttt
     1801 tgctgccaaa aaatttggcg taaaggtgga ggaattcggc attgggttgc ctccgcggtt
     1861 gtggggcaaa caaatagggg aaaccatata ttcggtgaac gcgcttccta tcggcggctt
     1921 tgtgcgcatg gaaggcgaag agaagcgctc tgaaagcccc cgaagcttta acaaaaaacc
     1981 ggtttggcag cggcttgtta tcgtgggcgg gggagttttt gtgttttggg ttattgctgc
     2041 agccattttt gccgggttgg gtgccaccgt aggcattccc atggcaatcg acgataacgc
     2101 ccagggagtt gccgacccac gagtgcaaat tataggcata gcttccaatt ctcccgcagc
     2161 agaggcgggg attgtattgg gcgataccat tttgcgcatt caaggccagg aaataacaaa
     2221 aatgcaggaa gtacggggta tcgcaacaac ccacgccggc caggaaatta cccttgaggt
     2281 acgaagagga accgaaaccc tgcagattaa ccttactccg cgcatagatc cgcccgaggg
     2341 agaaggcgca attggcgtgg cttttgtaag aaccggaatg gtgcactatg cctggtggca
     2401 agctcctttt cgcggaattg cgcatacggc acaacttacg gtggcaattc ttcgtacgtt
     2461 tgccgacctc atctcgggac ttgttgcagg gcaggggctg ccgcccggca cccagttttc
     2521 cgggcccatc ggcgtagtcg gctttttgag cgactcgctt ggactgggcg tttcctcgtt
     2581 tctctcgttt gtcgctttga tctcggtata tcttgccata ttcaatacgc ttcccattcc
     2641 cgctttagat ggcggcagaa tgttcttcct gcttgtggaa gccgttcggc gccgcccgct
     2701 gccagaaaaa atcgagcagc ggcttatcat tacctcgttc atcattctta ttgcgcttat
     2761 tatctggatt tccattggcg acgtgcgcaa tagattctag gttacaccat gaggcaatct
     2821 gaactgttta cgaaaacaca aaaaactctt ccgaaagacg aagagagcaa aaacgcaaaa
     2881 ctgcttttgc aggcgggctt tattcacaag gaaatggccg gcgtatactc ctttttgcct
     2941 ttgggcagac gcgtcatgga gaatattatg cagattatcc gcgaggaaat gaacgctttg
     3001 gacggccagg aaatattttt gacttcgttg caggattcgg ctccctggcg cgcatcgggg
     3061 agatggtcag acgaagtagt ggacgtgtgg tttaaaacaa agctcaaaaa tgatacagag
     3121 atcggccttg ccaacacgca cgaggaaccc ttaaccgcct taatgcggaa ccatattcag
//
LOCUS       DASRUP010000047         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1045436, whole genome shotgun sequence.
ACCESSION   DASRUP010000047 DASRUP010000000
VERSION     DASRUP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045436"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..540)
                     /locus_tag="VFE94_01815"
     CDS             complement(16..540)
                     /locus_tag="VFE94_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZX49871.1"
                     /translation="MNYFLLAVFALVLIWGIFFWGDPISFNLVPRARAPVQQQDSIRE
                     QIVQVLEQQVRPSATIKETQLELELARTPEEQSQGLSQRELLPENTAMLFVYEEPTIP
                     GFWMKDMNFALDMIWVDQNNAIIAVSENVAPESYPEIFFPPSLVKYVLEVNAGWAQAH
                     GVTVGDTVVFNGVF"
     gene            complement(537..1610)
                     /gene="ychF"
                     /locus_tag="VFE94_01820"
     CDS             complement(537..1610)
                     /gene="ychF"
                     /locus_tag="VFE94_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZX49872.1"
                     /translation="MALSVGIVGLPNVGKSTLFQTLTKKKVDTSNYPFATIDPNVGIV
                     KVPDDRVQKLADFFHSEKVVPTIIEFVDIAGLVRGANKGEGLGNQFLANIRETDAICQ
                     VVRCFQNANITHVDNTIDPVRDITTIQTELILKDLETVEKRLANLEKEARGQNKEAVK
                     KLEILQDLQKEMNKEKPAFQYIKEHVESAEVKEAVGELFLLSAKPVVYLMNADSEKEA
                     APAIAKAKELGAPYVIMNVKEEDDKVGLQPDELKEFGLSAPKLPELIKKAYEALHLIT
                     FFTTGPDETRAWTLPKGGLAPDAGGVIHSDFKEKFIRAAVIQWDKLLEAGGLAEAMAK
                     GLLRTEGKEYVVKDGDVIEIKHS"
BASE COUNT          564 a          610 c          490 g          636 t
ORIGIN      
        1 ctttgtttag taaccttaaa aaacgccatt gaatactaca gtgtcgccga cggttacgcc
       61 gtgagcctgc gcccagccgg cgtttacctc gagcacatac tttacaagag aaggcggaaa
      121 gaaaatttca ggataacttt ccggagctac gttctctgaa actgcaataa tcgcgttgtt
      181 ttgatctacc caaatcatat cgagcgcaaa gttcatgtcc ttcatccaga accccggaat
      241 tgtgggttct tcgtatacaa aaagcatggc cgtgttttct ggcagcaatt ccctttgcga
      301 aagaccctgg ctttgctctt ctggcgtacg agccaactca agttcgagct gcgtttcctt
      361 tatcgtcgcg ctcgggcgta cttgctgttc caaaacctga acaatttgtt cccgtatgct
      421 atcttgctgc tgaaccggag cccgcgcacg aggaactaaa ttaaacgaga taggatcacc
      481 ccaaaaaaat attccccaaa taagcacaag cgcaaatact gccaagagaa aatagttcat
      541 gaatgcttta tttctattac atccccgtct tttaccacat actctttgcc ttcggtgcgc
      601 agtaaacctt ttgccatggc ttcggccaaa ccgccggcct ccagcagttt atcccactgg
      661 attacggcag cgcgaataaa cttttcttta aagtcgctgt gaataacacc ccctgcgtcg
      721 ggcgccaacc ccccttttgg cagggtccat gctcgcgtct cgtcagggcc ggtagtaaaa
      781 aacgtaatta ggtgaagggc ctcatacgcc tttttgataa gctctggcaa tttcggcgca
      841 gaaagtccaa attctttgag ttcgtccggt tgcagcccaa ctttatcgtc ttcctcctta
      901 acattcatta taacatacgg cgcgcccagc tcctttgctt tggcgatggc aggcgccgct
      961 tctttttcgg aatctgcgtt cattaaatac accacgggct ttgccgaaag caaaaataat
     1021 tcccctaccg cctcttttac ctctgcagac tccacatgct cttttatgta ctggaacgcc
     1081 ggcttttctt tgttcatttc cttttgcaaa tcctgcaaaa tctcgagttt ttttacggct
     1141 tccttgtttt ggccccgcgc ctctttttct aaattcgcca agcgtttctc taccgtttcc
     1201 aagtctttta gaataagttc tgtttgaata gttgtgatgt cgcgcaccgg gtctatggtg
     1261 ttgtccacat gtgtaatgtt tgcgttttga aaacagcgta ccacctggca gatggcgtcg
     1321 gtttcccgta tatttgccaa aaactggttg cccaaaccct cgcctttgtt tgctccgcgc
     1381 acgagtcccg caatgtccac aaattcaata atagtgggca ccactttttc tgaatggaaa
     1441 aaatccgcga gtttttgcac cctgtcgtct ggcaccttaa caatgcccac gttggggtct
     1501 atggtggcaa acggataatt cgaagtatcc acctttttct tcgtaagggt ctgaaacaac
     1561 gtggatttcc ccacgttcgg caaccccaca attccaacag ataacgccat ggctttatcc
     1621 tactgcacaa ccccccgccc gacaagcccc aacttcccag ccgccgttct tgacatctgg
     1681 catcttaagc gtataataag ttccactgta cgaagtgttc ggattcaaat gcgaattcgc
     1741 caattaaagc accttaaaac tacgaagagg agttgagaat accatgaatt ggtggatagt
     1801 tggaggaatc ttagtagcgt atgcgttcat agggcttata gtaggaacct tcgctcttgc
     1861 gtggtcacaa ccagagcgca cgccttttgg accccctccc cccatagaga aaacggaccg
     1921 ccaaatagct gtagcatttg gcgtcctttg gccgattgcg ctttgtatct ttgtgtggat
     1981 actgcttcca aaaaggaaca caaagagcta aagcatatcg gcaacaaaca aaaggggcgg
     2041 gtctcaaagg agacaccgcc ctgaattttt atatgagata attgctaacg aattacggtg
     2101 ccggtaaaag gcttgccttc gatggcgttt tggttcggcg tatttacggc cggccaaaaa
     2161 gtcgcaggcc gttctttttg tagtcgctat caaagctcac aatcgcatca aaaccatagg
     2221 cggcggcaag agcgatattg cccgcatccg taaaactcac attctttgaa cgctgccgca
     2281 aaaaggtttc tttggctttc
//
LOCUS       DASRUP010000048         8403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1398714, whole genome shotgun sequence.
ACCESSION   DASRUP010000048 DASRUP010000000
VERSION     DASRUP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398714"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1449
                     /gene="gltX"
                     /locus_tag="VFE94_01825"
     CDS             1..1449
                     /gene="gltX"
                     /locus_tag="VFE94_01825"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZX49873.1"
                     /translation="MNHKVRTRMPPSPTGVAHMGTARTALFNYLFARKEKGAFVLRIE
                     DTDKERSKKEYEDDIVETLRWLGMEWDEGPDKGGPYAPYRQSERTELYKKYLAKLLDN
                     GQAYWCFCTTEELEAQRSHQTSLGEAPRYKGVCRSLTKEDQEKRLEAGDRGVIRFIVE
                     PKTLAFQDLIRGEITFDTGLLGDIVIAKDLETPLYNFTVVVDDFEMQMTHVIRGEDHI
                     ANTQKQILLQEALELPGLQYAHLPLLLGQDRTKLSKRHGDNSVRTYREAGYLPEAIVN
                     FLALLGWNPGDEREIFSMEELIQEFSLDRIQKGGAIFNPQRLDWINGIYIRNTPLDEL
                     AKLCQPYLPQSNTVDFETLKKIVALYHERLKHLSEITELTDFFFGDKLEYDKELLRWK
                     DATDSQTKEMLERLEGLIVGIQEPEWGREKLEKVIMPEAEKEKNRGYMLWPFRVALTG
                     KKASAGPFEIAEVLGKQKTLQRIQDAKAKLLP"
     gene            2108..2461
                     /locus_tag="VFE94_01830"
     CDS             2108..2461
                     /locus_tag="VFE94_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZX49874.1"
                     /translation="MMEISFEEFKKLDIRVGKIVQAERIAESEKLVKLLVDLGLPADE
                     AGEGEPRQIVAGIGLAYPNEADLVGKEIVVLCNLEPKTFLGHTSYGMLLAADSGGVPV
                     VLQPGQDVPPGSIIK"
     gene            2465..2935
                     /locus_tag="VFE94_01835"
     CDS             2465..2935
                     /locus_tag="VFE94_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49875.1"
                     /translation="MNNIVRHLMGDLGAGLILACALVPAISFAQSSDPTNLKAPETLE
                     EAKEGILNIGDKVAEGIPGIVASIWEDEILPLAKKMATFAKEEIWERRLKPLGARLRQ
                     EIGNLLGEEVEKRKPLIEEELEQEKQEVKEEFKEQAGEAGKGLWERFRGLFREE"
     gene            3087..4046
                     /locus_tag="VFE94_01840"
     CDS             3087..4046
                     /locus_tag="VFE94_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZX49876.1"
                     /translation="MKKSTKPILDHILDFLDYCEVERGLSNNTQRNYQRYLKKFEAWL
                     GKTSKLGLLPHELSADDVWQYRLFLSRSQDSSGKTLSKLTQNYYLIALRALLGFFVAK
                     DIQSLPPTKVTLPKGAEKERTIKFLEIDQIEKLLNSPNPNNPTGLRDKAILESFFSTG
                     MRVAELVSLNRDQFDSIADKQDFELGIIGKGSRPRTVYFSQRALEWLKKYLKTRGDKD
                     KALFINYRGRNQEERRLTARSIERIIKRYALQAGVPFFTTPHTLRHSYATDLLSQGVD
                     LRTIQEFLGHKTIATTQIYTHVTNKRLRDIHRQFHSGKNLKST"
     gene            complement(4110..4439)
                     /locus_tag="VFE94_01845"
     CDS             complement(4110..4439)
                     /locus_tag="VFE94_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49877.1"
                     /translation="MEPIEVFQKSKGDAAAYWEFTVLIGHDSKDVGFLVKIDPIHWEE
                     LTGGRVSPEHLIKKSFQFLLKKHRGVKYAIWRDFDLRQIQRSFPEYRDEMRKAARRTI
                     GFGMFSS"
     gene            complement(4702..7848)
                     /locus_tag="VFE94_01850"
     CDS             complement(4702..7848)
                     /locus_tag="VFE94_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZX49878.1"
                     /translation="MEKDKKSIWEFFLSHYRFTYIIIAAAISLGLFSLWQIPKESNPE
                     VNVPFATVTTVLPGAAATDVEELVTNVLEDKILSLSEIEEVSSTSREGISLIFVEFSA
                     SADADRKVDELKDKVDEAEANLPEETEDPVVTKIRFSDEAIVTFALSGPFPVAQLKQF
                     AEDLKSEIERISGVSRVEIVGGQEREIQVIVQRERLDTFGFGLGQVVNAISQANADIP
                     TGFIETGGANYTLRLQGALQGAADVSHVPIGVVGGTPVFVRDVADVIDGYQERTSISR
                     LSVGGAAPLPSISLNVFKTPGGNIVDVADAVEGVIAKGQQESLPDNIQIEVVQNTADF
                     IRQDLNTLSSSGLQTVLLLMILLLIFLGWREALLASIALPLTFLLTFIFLSAIGSTLN
                     FLSLFALILALGILIDSAIVMVQGMHEQITKQKKSPKEAAFATLRMFHNPLLAGTLTT
                     VFAFAPMLLMSGILGEFVKHIPITIIIVLSSSLFVAFALIPVIGMKWLRPQKEEAERM
                     QERKRGMGRFFKNVQLADRLAKEYEKLLVSLLESKRNKRRFVVVLVLLFVGSVALPFV
                     GALKINMFPDQEFATFYIDVSKPIGTPLRQTSLVAEQVEDILRQDPRIQSFVVQVGGA
                     SRTDIVAASSGGNLANIVVNLVPKEDREQKSYEIAEAYQREFTGSIHDAEVSVTSLSG
                     GPPTGAPVLITLKGESLEVLEQMARDFEAMLKEIPGTADIQSSVPESAGEFVLQIDRG
                     KAQLYGVSTVEVARLLRGAISGSTATVIRKGGEEMDVVVKFALNPATVQDGKTNIVDL
                     SSIESLTISTPTGDVPLSAFTKTELQGGRPIISHIDGERVMQVTSQTTGGATAQQIFA
                     RLQEKMEAIVVPQGYEVQMGGEVEDIQQSYNDMFRAMILAVFLIGASLVLEFRSFRQP
                     WFILSVIPLSLIGVLPGLVLLGQPLSFPGIIGIVALAGIVVNNAIILLDRTNENRRNG
                     MEIKEAVREAGKSRLQPILLTTITTVVGIIPITLSSELWASLGVSIIFGLSAATVMTL
                     FVVPMLYLRFAEKRLEAI"
     gene            complement(7852..>8403)
                     /locus_tag="VFE94_01855"
     CDS             complement(7852..>8403)
                     /locus_tag="VFE94_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZX49879.1"
                     /translation="IPAPISGVVATLPVRVGELAQMGQTVASIVNPGSVQVKTYLSGE
                     DARFVQQGARAVIEGEFEGAVTSVAPSLDPATKKVETLVGVEGRGNEDLVIGQFANVK
                     IFIENGLQNADTFFLPLQSIKVEPEASFVYTLSGDNVVERHQVELGRVIGNSVEVISG
                     IESGMTVVFSVRGLEPGDKVEIE"
BASE COUNT         2345 a         2070 c         1912 g         2076 t
ORIGIN      
        1 atgaaccaca aagtacggac aagaatgccg ccctcgccaa cgggagtggc acacatggga
       61 accgcaagaa ccgccctttt caactacctg ttcgccagga aagaaaaagg ggcttttgtc
      121 ttgcgcatcg aagataccga caaagagcgc tccaaaaaag aatatgaaga cgacattgta
      181 gaaacgctgc gctggctggg catggagtgg gacgaagggc cggacaaagg gggcccgtat
      241 gcgccctata ggcaatcgga acgcacggaa ctctacaaaa aataccttgc aaaactctta
      301 gataacgggc aggcgtactg gtgcttttgc accacagaag agctggaggc ccagcgctcc
      361 caccaaacga gcctggggga agctccgcgc tacaaaggag tatgccgaag ccttaccaaa
      421 gaagatcagg aaaagcgcct cgaggcaggg gataggggag tgatacgttt tatcgttgag
      481 ccaaaaaccc ttgcgtttca agatcttatt cgcggtgaaa taacattcga caccggattg
      541 ctgggcgaca ttgttattgc aaaagatttg gaaactccgc tgtataattt taccgtggtg
      601 gtggacgact ttgaaatgca aatgacccat gttattcgcg gggaagacca cattgccaat
      661 acgcaaaaac aaatactctt gcaggaagct ctggagctgc cggggctgca gtatgcccac
      721 ttgccattgc tgctcgggca agaccgcacc aaactttcaa aacgccacgg cgacaactcg
      781 gtgcgcacat accgcgaggc agggtatttg ccagaagcga tcgtaaactt tctggcgctt
      841 ttggggtgga acccgggcga cgaacgcgag attttttcta tggaagaact tattcaggag
      901 ttttcgctcg accgaattca aaaaggagga gcgatattca accctcaaag gttagactgg
      961 ataaacggca tatatattcg gaacacgccg ttggacgaac tcgccaaact ttgccagccc
     1021 tatttgcccc aaagcaacac tgttgatttt gaaacgctca aaaagattgt cgcgctgtac
     1081 cacgaacgtt taaagcatct ttctgaaatt acagaactta cggatttctt ttttggagac
     1141 aaactcgaat acgacaaaga attattgcgc tggaaagacg cgacagacag ccaaacgaaa
     1201 gaaatgttag agagactaga gggtttgatt gttggcatac aggaaccaga gtgggggaga
     1261 gagaagctgg aaaaagttat aatgccggaa gcagaaaaag aaaaaaacag gggatacatg
     1321 ctgtggccgt ttcgcgttgc gctcaccgga aagaaagctt cggcagggcc atttgaaatt
     1381 gcagaggtct tgggcaaaca aaaaaccttg caacgcatac aagatgccaa agcaaaactc
     1441 ttaccataat attgtgcgac attttgcctc ttgattttaa gcccgttttt tgatataggt
     1501 agaaactaat agctctatat agaggtgatg ttgtggcaag gaaaagaaca agacggcaac
     1561 aaagaacaac tctttggggc agactaaagt cttggttcgg cttttcttct tcaaacaaaa
     1621 aagtccctaa atacaaacga caccgccgtg agcgaagacg gctaaacgac gatatcttta
     1681 ggacggggag aagtaggtag agttataaaa tagcaccaaa ccatacgttg ggtcctcagt
     1741 cacatgcgga cccttttctt tttgagtcac atcggagttg aaaaataagg gaaaatatgc
     1801 gacagtagaa ttatgcttaa gaagactgaa tttgattttg aaactgagga ctttggcgag
     1861 tttcgaaaaa gatcagtcag taccaaaagt gtgtagcatt ttttaaggaa gttcatgaat
     1921 ctagggtaaa gataggggaa cagataacac tagttatccc agctcattca gtctttgaaa
     1981 ttaatacaaa gcttagaaaa agaagaagac agcacgattg ggaatctttc ccttcgttta
     2041 ctgccgaagg tgcgcaattt tatcaaatta tgcatgattt tattaaagaa ctgcaaaaga
     2101 aaaagctatg atggaaatat catttgaaga gtttaagaaa ctggatattc gcgtggggaa
     2161 gattgtgcag gcggaacgga ttgcagaatc ggaaaagctg gtgaagcttt tggttgacct
     2221 aggcctgccg gccgatgagg caggagaagg ggagccgagg cagattgtgg ctggcattgg
     2281 cttggcatac ccaaacgaag cagatttggt tggcaaagaa attgtcgtgc tgtgcaactt
     2341 ggagccaaaa acatttttgg gccacaccag ctacggcatg ttgctggcgg cagacagtgg
     2401 gggagtacct gtggtgctgc aacctggtca ggacgttccg cccggcagca tcataaagta
     2461 acaaatgaac aacattgtgc gacatttaat gggtgatcta ggggcagggc ttatactagc
     2521 atgcgccctt gttcctgcga tttcttttgc gcaatcttca gaccccacta acctcaaagc
     2581 tcccgaaact ctcgaggaag ccaaggaggg gattttgaat ataggcgata aagtagccga
     2641 aggcattcca ggcatagtgg caagtatttg ggaggatgaa atcttgccgc tcgcaaagaa
     2701 aatggctact tttgccaaag aagagatctg ggaaaggcgt ttgaagcctt taggggctcg
     2761 tttgaggcag gaaataggca atctgctcgg agaggaggtt gaaaagcgaa aacccctcat
     2821 cgaggaagag ttagagcagg aaaagcagga agtcaaggaa gagttcaagg aacaggccgg
     2881 ggaggcagga aaagggctct gggagcgttt cagaggcctt ttccgcgagg aataggtagt
     2941 ctgatacctc ctaaagacag gggtgcaaaa agggtgttga cagcctttgg ggaagcatac
     3001 tatgataaac ctaccaaatg tcgtacaatg tcagtaaacc gaccattagg cttattcgtt
     3061 tgtaaaaagg cctcccctag cgaaatatga aaaaatccac aaaacctata ttagatcata
     3121 ttctagattt tcttgattac tgcgaagtag agcgcggact ttccaacaac acgcagcgca
     3181 attatcagcg ctaccttaaa aaattcgagg cgtggcttgg taaaaccagc aaattaggtc
     3241 ttttgcccca tgagctatct gccgacgacg tttggcaata ccggctgttt ttgtcgcgtt
     3301 cccaggattc ctcgggcaaa actctgtcca aactgacaca aaactactat ttaatagccc
     3361 tacgggctct tttgggcttt tttgtggcaa aagacataca aagcctgccg ccaaccaagg
     3421 taacgctgcc aaaaggcgct gaaaaggagc gcaccataaa gtttttggaa attgaccaaa
     3481 tagaaaagtt attaaactcc cctaacccaa acaatccaac cggcctgcgc gacaaagcaa
     3541 ttttagaatc ttttttttct acggggatgc gcgttgcaga actcgtgagc ctcaaccgcg
     3601 accagttcga ttctatcgcc gacaaacaag attttgagct gggcattatt ggcaaaggca
     3661 gccggcctcg caccgtgtac ttttcccagc gcgctttgga atggcttaag aaatacctaa
     3721 aaacccgtgg cgacaaagac aaagcccttt ttattaatta ccggggccga aatcaagaag
     3781 aacgccgtct tacggcccgc tctattgaac gaattataaa acgctatgct ttgcaggcag
     3841 gagtcccctt ttttactacg ccccacacgt tgcgccattc gtacgctact gaccttcttt
     3901 ctcaaggtgt tgatctgcgc actattcagg aatttcttgg gcacaaaacc attgctacca
     3961 cgcaaattta cacgcacgtt accaacaaac gcctgcgcga tattcaccgg caatttcaca
     4021 gcggcaaaaa tttaaaatct acatgaaact acaacaaaat ccgggtttcg tatggcccgg
     4081 attctttttc accgaaatgt cgcacaatat taggagctaa acatgccaaa gcctatggtt
     4141 cgccgcgcag cttttcgcat ttcatctctg tattccggga aactgcgctg aatctgccgc
     4201 aggtcgaaat cccgccatat tgcatattta actccccggt gtttttttaa caaaaactga
     4261 aacgattttt taattaagtg ctctggcgat acccgcccac ccgtaagctc ctcccaatgg
     4321 atagggtcta tctttaccaa aaacccgaca tcttttgagt catgtcctat aagaaccgta
     4381 aattcccaat atgccgctgc gtcccctttt gatttttgga agacttctat cggctccatg
     4441 gggcttttgg aagtaagctt gttattcact tcgttgagta ttcttgattg caagctactc
     4501 tgcaaactcc cctatcctag tttgcaagca agaatattca agttgtaaaa caaaaaagct
     4561 gctttccaac ggccttctag gctctctact tccatgaaaa caaaaccaaa cgttattgtc
     4621 aagtactttc taaagaaaaa cgcttaaagt tgtttatttt cttaaccact cccctacttt
     4681 ctctttagtt cttaaaaaat attatatggc ttcgaggcgt ttttcggcaa agcgcaaata
     4741 gagcatgggc accacgaata gagtcattac ggtggcggcc gacaagccga atataataga
     4801 cacgccgagc gacgcccaca gctccgagct caacgtaatg ggaataatgc caacaaccgt
     4861 agtaatggtg gtaagcaata tgggctgtaa acgagacttc cccgcttccc taaccgcttc
     4921 ctttatttcc ataccgttcc tgcgattttc gttggtgcga tcgagcaaaa taatggcgtt
     4981 gtttaccaca attcccgcta acgccacaat gccgataatt cccggaaaac ttaagggctg
     5041 gcctaaaagc accaggcccg gaagtacacc aataagcgac aagggaataa ccgacaaaat
     5101 aaaccacggc tgcctgaacg agcgaaattc caatactaac gaggcgccaa ttaagaacac
     5161 ggctagaatc atggctctga acatatcgtt atacgattgc tggatatctt ctacttcccc
     5221 gcccatttgc acttcgtagc cttgcggcac aacaatggcc tccatttttt cttgaagtct
     5281 cgcgaaaatt tgttgcgccg tagctccacc tgtagtttgg cttgttacct gcataactct
     5341 ttcgccgtcg atatgggaga ttatcggcct acctccctgc agctcggttt tggtaaacgc
     5401 gcttaggggc acgtcgcccg tgggcgttga aatggttaac gattcaatgg aacttagatc
     5461 cacaatgttg gttttgccgt cttgtacagt tgcgggattt aacgcaaact ttaccaccac
     5521 gtccatctct tcgccccctt ttcgaattac cgtagccgta cttccgctta tggctcctcg
     5581 caacagacga gccacctcaa cggtgcttac tccatatagc tgggctttcc ctctatcgat
     5641 ttgcagcaca aactctccgg cggattctgg cacgctgctt tgaatgtcgg cggttccggg
     5701 aatttctttg agcattgcct caaagtctct tgccatttgt tccaatacct ccaaagactc
     5761 ccctttcaag gtaatgagca cgggggctcc cgtaggcgga ccgccagaca aactggttac
     5821 tgaaacctcg gcatcgtgaa tgctccccgt aaactctctt tggtaggctt ccgcaatttc
     5881 gtagctcttt tgctctcgat cctctttggg aaccaggttt acaacaatgt ttgccaaatt
     5941 ccctcccgaa cttgctgcta caatgtcggt tctggaagct cctcccacct gcacaacaaa
     6001 cgattgtatt ctgggatcct ggcgcaatat gtcttccact tgctctgcca caagagatgt
     6061 ctggcgcaaa ggcgtcccaa taggttttga aacgtcgata taaaaagtcg caaattcctg
     6121 atccgggaac atgttgattt taagcgcccc tacaaaaggc agcgccacgc tcccaacgaa
     6181 caaaagaacc aaaacaacta caaatcttct tttgtttctc ttgctttcca aaagcgacac
     6241 caaaagcttt tcgtactctt ttgcaagccg atcggctaac tgcacatttt tgaaaaacct
     6301 tcccattcct cttttccgtt cttgcatgcg ttccgcttct tctttttgcg gacgcagcca
     6361 cttcatgcct ataactggaa taagcgcaaa cgccacaaac agcgagctcg acagcacaat
     6421 aataatggta ataggaatgt gttttacaaa ttcccccaaa atgccggaca ttaacagcat
     6481 gggagcgaac gcgaacaccg tggttaaagt tcccgccagc aaagggttgt ggaacatgcg
     6541 gagcgtggcg aaagccgctt ctttgggcga tttcttttgt ttggtaattt gctcgtgcat
     6601 tccctgcacc atgacaatag cgctgtcgat taaaattccc aaagctaaaa tgagcgcgaa
     6661 caaggaaagg aagttaagcg ttgaaccaat cgcagaaaga aaaataaaag taagcaaaaa
     6721 tgtaagcggc agggcaatcg aagccagcag agcttctctc caccccaaaa aaatgagcag
     6781 tagaatcatg agcaacagca ctgtttgcag gccgctggaa gaaagagtgt ttaggtcttg
     6841 gcggataaaa tctgccgtgt tttgcaccac ttctatttgt atattgtctg gcagcgattc
     6901 ttgctgcccc tttgcgatga cgccttctac cgcatctgcc acgtccacaa tgtttcctcc
     6961 gggcgtttta aaaacgttga gcgatatgga aggtaaggga gctgctcccc ctacgctcaa
     7021 acgggaaata cttgttcgtt cttggtagcc gtcgattaca tcggctacgt ctcttacaaa
     7081 taccggagtt ccgcccacga cgccaattgg cacatgagaa acatctgctg cgccctgcag
     7141 tgctccttgg agacgcaggg tataattggc gcccccggtt tcaataaatc ccgtgggaat
     7201 atccgcattc gcctggctta tggcgttgac aacctggccc aaaccaaagc caaacgtatc
     7261 cagcctttct ctttgcacaa taacctgtat ttcgcgttcc tggccgccca cgatttctac
     7321 gcgcgaaacc cccgatatcc gttcgatttc gcttttcaag tcttcggcaa actgcttaag
     7381 ctgggccacc gggaaaggcc cgcttaacgc aaacgttaca atagcttcgt cggaaaaccg
     7441 aatttttgtt actacgggat cctctgtttc ttctggcaaa tttgcctctg cttcgtctac
     7501 cttgtctttt aactcatcta ctttacggtc ggcgtccgcc gaggccgaaa attccacaaa
     7561 aatcaacgaa atcccctccc ttgacgtgga acttacctct tctatttcag agaggctcaa
     7621 aattttgtct tccagcacat tggttaccag ttcttccaca tcggtcgcgg cggctccggg
     7681 caataccgtg gttaccgtgg caaacgggac attcacttcg gggtttgatt ctttggggat
     7741 ttgccacaac gaaaacagcc ccaatgaaat ggcggccgca ataataatat aggtaaaacg
     7801 ataatgggaa agaaaaaatt cccaaatact ttttttgtcc ttttccatgg gttactcgat
     7861 ttccacttta tctccgggct ccaaaccgcg caccgaaaac accacagtca tgccgctctc
     7921 aattccgctt attacctcta cggagttccc tattacccga cccagctcta cctggtggcg
     7981 ctccacgacg ttgtcgccag aaagcgtgta cacgaacgac gcttcgggtt ccacttttat
     8041 agactgcaaa ggcaggaaaa aggtgtcggc attctgcaag ccgttttcga taaaaatctt
     8101 tacgtttgcg aactgaccga ttaccagatc ctcattcccg cgcccttcca ccccaaccaa
     8161 tgtttctact ttcttggtcg caggatccaa actgggggcc acgcttgtaa ctgcgccttc
     8221 aaactccccc tcgataacgg ctcttgctcc ctgttgcaca aaccgcgcat cctcgccgct
     8281 caaatacgtt tttacctgca cactgccagg atttacgata gaggcaactg tttgccccat
     8341 ttgagccaat tctcctacac gcacaggaag ggtggcaacc acgccggaaa tgggtgctgg
     8401 aat
//
LOCUS       DASRUP010000049         8009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_645492, whole genome shotgun sequence.
ACCESSION   DASRUP010000049 DASRUP010000000
VERSION     DASRUP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645492"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFE94_01860"
     CDS             <1..1032
                     /locus_tag="VFE94_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HZX49880.1"
                     /translation="APWRASGRWSDEVVDVWFKTKLKNDTEIGLANTHEEPLTALMRN
                     HIQSYRDLPVYVYQFQTKLRNELRAKSGIMRTREFIMKDLYSFCRTKEEHEQFYELAK
                     QSYLKIFDRVGLGEQTYVTAASGGSFSKYSHEFQTVCEAGEDTIYIDEQQRTAVNKEI
                     IDEPEYDKSKLKEAKAVEVGNIFTLGTKYSEALSLLYQDEQGKKHPVFMGSYGIGPAR
                     TMGTIAEVYADEKGLVWPEQVAPFLVHLIALPGVEAKADGLYDTLQKQGIEVLYDDRQ
                     HVQPGAKFADADLIGIPYRAVVSEQTVKQNSVEVKKRLEQNPQLIPWEQSYEFFKQHS
                     FASRQGHRN"
     gene            980..1960
                     /locus_tag="VFE94_01865"
     CDS             980..1960
                     /locus_tag="VFE94_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZX49881.1"
                     /translation="MNFLSNILSRLGKDIAIDLGTANSLVYVKGRGIVLQEPSVVAVN
                     QKTGKVLAIGEEAKKMVGRTPLHIVATRPLVKGVISDFEVTEQMLKYFITKAYEKKFI
                     LSPGPRVIVGIPFGVTEVEKKAVSDATLNAGGREVFLIEEPMAAAIGARLAVQEPGGN
                     FIVDIGGGTTEVAVLSLGGVVYAKSIRIAGDKLNEDIINYAQEEYKLLIGERTAESLK
                     IAIGSASPLKEKKEMPLRGRNLVTGLPEEIVVSDDDIRKAMERSVRQIVNEIKATVEE
                     TPPELLADIMHRGIFLAGGGALLRELDTLITKETKIPARIVEDPLTAVVR"
     gene            2044..2331
                     /gene="gatC"
                     /locus_tag="VFE94_01870"
     CDS             2044..2331
                     /gene="gatC"
                     /locus_tag="VFE94_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZX49882.1"
                     /translation="MIQRSDVEHIAKLARLELTPQEIEQYQNDLSFVLEYFEILGELD
                     VSNVQPMTHSVAHQNVKRKDEAGTKIRARAAQLLEMAPETKDGFVKVKSIL"
     gene            2338..3759
                     /gene="gatA"
                     /locus_tag="VFE94_01875"
     CDS             2338..3759
                     /gene="gatA"
                     /locus_tag="VFE94_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZX49883.1"
                     /translation="MERLFQLSISQIHEKLVGKEVLATEITEAFLQRIKEKDGDLNAF
                     ITQTPELALEQARHVDEKIAKGEAVSALAGIPCAVKDSILLQGVRCTAASKILENYVA
                     PYDATVIQKLKEQDVVFIGKTNTDEFNMGASTENSAFGPTKNPYDLTRVAGGSSGGSA
                     AAVAATEAHIALGEDTGGSIRLPAAFCGIVGLKPTYGAVSRHGVIGIASSLDQVGPMA
                     RTAEDAETMFQAMCGKDELDATSLEYVYEPMHDFDLKKLRIGVPKEYFGEGLAPEVKT
                     VIEKAIGKLKEAGAQVKELSLPTSKYALAAYYIINPSEISANLARYDGIRYGAWESAP
                     NLLDTYRKTRGKYFGAEVKRRIMLGTYALSAGYYDAYYLKAQKIRSLLKREFDEAFQN
                     VDVIAGPVSPTLPFKIGEKAQDPLAMYLVDLYTVPVNLAGLPALSLPAGRAGDLPVGL
                     HLIAKPLAENLLFATAKTFEKLL"
     gene            3776..4303
                     /locus_tag="VFE94_01880"
     CDS             3776..4303
                     /locus_tag="VFE94_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49884.1"
                     /translation="MEQVFEILNAINSPAVQNWGIIPWIRAICVFISAFFITIVIVSI
                     TRTPYLQLSAVGDTVEIATFRPWGVPKILRRWKRILARLDRGTEAEYKLAIMESDVLL
                     DEMLKKMRLKGDTVEERLGRITSIMIPNVEELRGAHEIRNSIVYDPDYRISLMETRRV
                     LEVYQRTFEQLDLFR"
     gene            4333..4403
                     /locus_tag="VFE94_01885"
     tRNA            4333..4403
                     /locus_tag="VFE94_01885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4365..4367,aa:Met,seq:cat)
     gene            4420..4491
                     /locus_tag="VFE94_01890"
     tRNA            4420..4491
                     /locus_tag="VFE94_01890"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4452..4454,aa:Met,seq:cat)
     gene            4496..4573
                     /locus_tag="VFE94_01895"
     tRNA            4496..4573
                     /locus_tag="VFE94_01895"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4531..4533,aa:Asp,seq:gtc)
     gene            4587..5216
                     /gene="pyrE"
                     /locus_tag="VFE94_01900"
     CDS             4587..5216
                     /gene="pyrE"
                     /locus_tag="VFE94_01900"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZX49885.1"
                     /translation="MDYAQQIAQALLHIGAVGFCVEDPITFTSGIKAPVYVDNRRFPF
                     WPAEWHLVIDAFVQKIKDENLTFDIIAGIETAGIPHSSALAYVLQKPSVFVRKKPKEH
                     GTKSKIEGGNVQGKKVLLLEDLVTTGGSSLAGVEALRAEGAQVTDCLVIVTYEFSESQ
                     AAFNDAQVQLRALTSFPVILQEAKQAGKITPAEKQTIEQWLSNPHEWRN"
     gene            5200..6003
                     /gene="pyrF"
                     /locus_tag="VFE94_01905"
     CDS             5200..6003
                     /gene="pyrF"
                     /locus_tag="VFE94_01905"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZX49886.1"
                     /translation="MSGAIEKYNNRADKVNSLLCAGLDPDFEKLPERFQKLEFPQFEF
                     NKWIIGQTAKYAAAYKPNMAFYESRGDRGIRELKMTMEYLQENYSDIFTICDAKRGDI
                     GSTSEHYAKSVFDWFGFDAVTLNPYLGKDALQPFLDYKEKGCIILARTSNPGAREFQD
                     QLIGQKPLWHVVAETVAKNWNENNNCMLVAGATYPKELKTIRSIAGDMTLLVPGIGAQ
                     GGDVKAAVEAGLNSQGKGLIMSTGRAILFAENPGQAARTLRDEINSFRS"
     gene            complement(5989..6858)
                     /gene="prs"
                     /locus_tag="VFE94_01910"
     CDS             complement(5989..6858)
                     /gene="prs"
                     /locus_tag="VFE94_01910"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZX49887.1"
                     /translation="MFLIPTLQAQHLAKKIKKSGLSPIFPAVNKEGARHFPDGELYVK
                     IPQASKLSGKHVVVLHSGAPSPNEGLIELELILQTLKEVKASPTDVFFAYFPYGMQDE
                     VFARGETNVAENLIEKLYKYYGVRNIYIIDAHFAGKKWAERYPINHLSVVPQLIAAAK
                     RDFGDDLLFLSPDKGGARRTKIQGVNKKRKNSYEIEIQSSASLRQNIRNRPIAVVDDL
                     IETGGTLDRFADECKKAGAKKVVALATHGVLPQGIARVKKKYKKLYLTNTINRPQANV
                     DISSLIVKSLGTK"
     gene            6922..7785
                     /locus_tag="VFE94_01915"
     CDS             6922..7785
                     /locus_tag="VFE94_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZX49888.1"
                     /translation="MTWYNLALFSLFAYGIQNFLYKVAAEKKCNTAWVMVAMMTTITV
                     LGAVFFVISGAQITNLSLLLLLSFTGGPLFLASLAARTEALKNASTSVVYPITRFSVI
                     LVVLFSIVYFREHLSMYQIAGIGLAVIVFVLLTRHNHEERARHANFRAGVILAVIALV
                     TGAGATIISKFAAESFEEFFSFIAISNAINIFLALGIKNKLQRERENPKHTNAIIIGV
                     LSGLFNFVGYYAIMHALAAGPLSIIAAVHSMNFVIAILLAVLIYKEELTKTRLAGIGL
                     TVLALILLRLS"
     gene            7790..>8009
                     /locus_tag="VFE94_01920"
     CDS             7790..>8009
                     /locus_tag="VFE94_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49889.1"
                     /translation="MTKDSSGEEQHEQWLGKLQGTKEYAFDRPALHTEVFGLQFANPI
                     GLAAGFDKHATALPQLASLGFGFLEVGTV"
BASE COUNT         2148 a         1891 c         2036 g         1934 t
ORIGIN      
        1 gctccctggc gcgcatcggg gagatggtca gacgaagtag tggacgtgtg gtttaaaaca
       61 aagctcaaaa atgatacaga gatcggcctt gccaacacgc acgaggaacc cttaaccgcc
      121 ttaatgcgga accatattca gtcgtatcgc gatttgcccg tgtatgtata ccagttccag
      181 acgaaactgc gcaacgaatt gcgcgccaaa agcggcatta tgcgcacgag ggagtttatt
      241 atgaaagact tgtactcttt ttgccgcacc aaagaggaac acgagcaatt ctacgagctg
      301 gcaaagcagt cgtatctcaa aatctttgac cgcgtgggat tgggagaaca aacgtatgtt
      361 acggcagctt cgggcggcag tttttcaaaa tactcgcacg aattccaaac agtgtgcgaa
      421 gccggcgaag ataccattta cattgacgag cagcaacgaa ccgccgtaaa taaagaaatc
      481 atcgacgaac ccgaatacga caaatcaaaa ctaaaggaag ccaaagcagt ggaggtgggc
      541 aacattttta ctcttggcac aaaatattcc gaggcgctct cgcttttata ccaggacgag
      601 cagggtaaaa aacacccggt ctttatgggg agttacggaa ttggccccgc ccgcaccatg
      661 ggcactatag ccgaagtgta cgccgatgaa aaaggcctgg tgtggccaga gcaggtggcg
      721 ccttttttgg tgcaccttat cgctttgccg ggggttgaag caaaagcaga cgggttgtat
      781 gacactttgc aaaaacaggg gattgaggta ttgtatgacg ataggcagca cgtgcagcct
      841 ggcgcaaagt ttgccgacgc cgatttaatc ggcattccat accgcgcagt ggtttcagag
      901 caaaccgtta agcaaaacag cgttgaagta aaaaaacgtt tagagcaaaa tccgcaatta
      961 attccttggg aacaatcgta tgaatttttt aagcaacatt ctttcgcgtc tcggcaagga
     1021 catcgcaatt gatttgggca ccgcaaactc gctggtgtat gtaaaagggc gaggcatagt
     1081 tctccaggaa ccctcggttg tcgcggtaaa ccaaaaaacc ggcaaagtgc tggccattgg
     1141 agaggaagcg aaaaaaatgg tgggccgcac acccctgcat attgtggcca cgcggccgct
     1201 cgtaaagggg gtgatctcgg actttgaagt aaccgagcaa atgctcaagt actttattac
     1261 caaggcgtat gagaaaaagt ttatcttaag cccgggtccg cgcgttattg tgggaattcc
     1321 ttttggcgta accgaagtgg aaaagaaagc cgtgtcagac gccacgctca acgcaggggg
     1381 gcgggaagta tttttaattg aagaacccat ggctgctgcc attggcgcaa ggctggccgt
     1441 gcaggaaccc ggggggaact ttattgtgga tatcggagga ggaaccaccg aagtggctgt
     1501 gctttcgctg ggcggcgtgg tatacgccaa atcgatccgc attgcgggcg acaagctcaa
     1561 cgaagatatt atcaactacg cccaagagga atataaactg cttattggcg agcgcaccgc
     1621 agaatcctta aaaattgcga ttggatcggc ctcacctctc aaggaaaaga aagaaatgcc
     1681 cttgcgcggc cgcaatcttg ttaccggttt gcccgaagaa atcgtagtgt cagacgacga
     1741 tattagaaaa gcgatggagc gttccgtgcg ccaaattgta aacgaaatta aagcaactgt
     1801 cgaagaaacg ccgcccgaac ttttggcgga tattatgcac cggggaattt ttcttgcggg
     1861 cgggggagcg ttgctgcgag agctggacac cttaattacc aaagaaacca aaatcccggc
     1921 ccgcatcgta gaagatccct tgactgccgt ggtgcggtga gccgagctcg tgctcgaaaa
     1981 cttagacgag cttcgtgata ttttggtgga agcggaagat ttagaaccgc ccagataact
     2041 gccatgatac agcgttccga tgtagaacac attgccaaat tagcgcgcct tgagcttacg
     2101 ccccaggaaa tcgaacaata ccaaaacgat ttgtctttcg ttttggaata ttttgaaatt
     2161 cttggcgaac tcgacgtctc gaatgtacag cctatgacgc actcggtcgc gcaccaaaac
     2221 gtaaagcgga aggacgaagc tggaacaaaa atacgcgcca gggcagccca actcctcgaa
     2281 atggcccctg aaacaaaaga cggttttgta aaagtgaagt ctatcttgta gcaaaacatg
     2341 gagagattat ttcagctgtc tatttcgcaa attcatgaga agctcgtggg caaggaggtt
     2401 ttagcaaccg aaattacaga agcttttttg cagcggataa aagaaaaaga tggcgaccta
     2461 aacgctttta ttactcaaac tccggaactg gcgctagaac aagcgagaca cgttgacgaa
     2521 aaaatcgcca aaggggaggc tgtttctgcg cttgccggca ttccctgcgc agtcaaagat
     2581 tccatattgc tccagggcgt gcgttgtacc gcagcctcca agattctcga aaactatgta
     2641 gctccttacg acgcaacggt aattcaaaaa ctaaaagagc aggatgttgt ttttataggt
     2701 aaaacaaaca ccgatgaatt caacatgggg gcttccaccg aaaactctgc tttcggcccc
     2761 accaaaaacc cttatgattt aacgcgcgtg gccggcggca gttcaggagg atcggctgct
     2821 gccgtagctg cgacagaagc ccatattgcg cttggcgaag acaccggagg ttccatacgg
     2881 cttcccgcgg cattctgtgg gatagtaggc ttaaagccaa cgtacggcgc ggtttccagg
     2941 catggtgtaa tcggtatagc ttcgtctctt gaccaggtag gccctatggc aagaaccgca
     3001 gaagatgcgg aaaccatgtt tcaggctatg tgcggaaaag acgaactcga cgccacatcg
     3061 cttgaatatg tatacgaacc catgcacgac ttcgacctca aaaaactgcg cattggcgtg
     3121 cccaaagagt attttggcga gggtctggcg cccgaggtaa aaaccgtaat tgaaaaagca
     3181 atcgggaaac tcaaagaagc aggggctcaa gtaaaagagc taagccttcc caccagcaaa
     3241 tacgcgctgg ctgcgtatta tatcattaat ccttcggaaa tttcggcaaa ccttgcaaga
     3301 tacgacggca tccgctatgg ggcttgggaa tccgcgccaa atctgctgga tacctaccgt
     3361 aaaacgcggg gcaagtattt tggagcggag gtaaaacgcc gcattatgct gggcacctat
     3421 gcgctttctg ccgggtacta cgacgcctat tatctcaagg cgcaaaaaat cagaagcctc
     3481 ttaaaacggg agttcgacga ggcctttcaa aatgtagacg taatagcggg ccccgtaagc
     3541 cctacccttc cttttaaaat tggggagaag gcgcaggacc cgcttgccat gtacctggtt
     3601 gatctgtaca ccgttccggt gaaccttgcg gggctgccag ccctgtctct acccgcagga
     3661 cgtgcgggcg acctgcccgt agggctgcat ctcatcgcaa agccgcttgc agaaaacctt
     3721 ttgtttgcaa cggcaaaaac tttcgaaaaa cttctgtaag tattataatg aggttatgga
     3781 gcaggttttc gaaattctca atgccataaa ctctcccgct gttcagaact ggggcatcat
     3841 accctggatt agagctatat gtgtttttat atcagcgttc tttatcacga ttgttattgt
     3901 ttctattacc cgaacgccgt atctccagct aagcgccgtg ggcgatacag tggaaattgc
     3961 cacattccgg ccctggggag ttcccaagat tttgcggcga tggaaacgta ttttggcgcg
     4021 cttagatagg ggaacggaag cagaatacaa actcgccatt atggagtccg atgttttgct
     4081 cgacgaaatg ctcaagaaaa tgcgcttaaa aggcgacacg gtagaggagc gtttgggccg
     4141 cattacctct attatgatcc ccaatgtcga agaacttcga ggcgcccacg aaatccgcaa
     4201 cagcattgtg tacgacccgg actaccgcat aagcttaatg gaaacgcggc gcgtgctcga
     4261 ggtatatcag cggacatttg aacaacttga tttgtttcgg taaatcgcct atactataag
     4321 gcgaaccact cgcgcggggt agtggagtag taccatgcga ggcccataac cttgtgacgc
     4381 cggtgcaaat ccggcccccg caacatcgta tagaggcctg gctgcgtagc tcagtggtag
     4441 agcgaggctt tcataaggcc gaggtcgcaa gttcaattct tgccgcagcc acattgcagg
     4501 cgtagcttag ctggttcaaa gcgcttccct gtcacggaag agatcgccgg ttcgaatccg
     4561 gtcgcctgcg ccacgataaa tcttctatgg attacgccca acaaatcgca caagccctgc
     4621 tgcacattgg cgccgtaggg ttttgtgttg aagatcccat aacgtttaca tcgggcatca
     4681 aggctcccgt atatgtagac aatcgcagat ttcctttttg gccagcagag tggcatttgg
     4741 tgatcgatgc ttttgtccag aaaatcaaag acgaaaacct tacattcgac ataatagcag
     4801 gcattgaaac tgcgggcatt ccccatagtt cggcgctcgc atacgtgttg caaaagccct
     4861 cggtgtttgt gcgcaaaaag ccaaaagagc atggcaccaa gagcaaaatc gaagggggaa
     4921 acgtgcaagg caaaaaagtg ctgcttctcg aagacttggt aaccacaggg ggcagtagct
     4981 tggctggggt agaggcgctg cgcgcagaag gcgctcaagt aaccgattgc ctggtcattg
     5041 ttacctatga attttccgaa tcccaagcgg cttttaatga tgctcaagta cagcttcgcg
     5101 ctttaacatc gtttccggtt attctccaag aagcaaagca agcagggaaa atcacgccgg
     5161 cagagaaaca aactatagaa caatggcttt ccaaccccca tgagtggcgc aattgaaaag
     5221 tacaacaacc gggcagataa agtaaactcg cttttgtgcg cggggctgga tcccgacttt
     5281 gaaaagctgc ctgaacgctt tcaaaaactc gagtttcccc agtttgaatt caacaaatgg
     5341 attatcggcc aaaccgcaaa atacgcggca gcttataaac ccaacatggc gttttatgag
     5401 tcgcggggag ataggggaat acgcgaactc aagatgacta tggagtattt gcaagaaaac
     5461 tactctgata tttttaccat ttgcgacgcc aaacgtggcg atattgggtc taccagcgaa
     5521 cactacgcca aatcagtttt tgactggttt ggattcgacg ccgtaacctt aaacccgtat
     5581 cttggtaaag atgctctaca gccctttctg gattacaaag aaaaaggctg tattattttg
     5641 gcgcgaactt ctaatccagg cgcaagagaa tttcaagatc aactcatagg gcaaaagccg
     5701 ttatggcacg ttgtcgcaga aaccgtggca aaaaactgga acgaaaataa caactgcatg
     5761 ctggtggcag gcgccacata ccccaaagaa cttaaaacaa ttcgcagcat tgcaggcgac
     5821 atgactttgc ttgtgcctgg cattggggcg caagggggcg atgtgaaggc cgcggtggag
     5881 gcagggctta actcgcaagg caagggcctt attatgagca ccggccgcgc tatcttgttt
     5941 gcagaaaacc cgggccaagc tgcccgcacc ctccgcgacg aaatcaactc atttcgttcc
     6001 taaagatttt acgataagag aagagatatc tacgtttgcc tgggggcggt tgatggtgtt
     6061 agtaaggtac aatttcttgt atttcttttt aactcgggcg ataccttggg gcaatacgcc
     6121 gtgggtcgct aaagctacaa cctttttggc gccggccttt ttgcattcgt ctgcaaacct
     6181 gtcgagcgtg ccgccagttt cgattaagtc gtccaccaca gctatggggc ggttgcggat
     6241 gttttggcgc agactcgcag aactttggat ttcaatttca taggagtttt tgcgcttttt
     6301 gttaacaccc tggattttgg tacgccgcgc gccgcccttg tcgggcgaga gaaaaagcag
     6361 atcatcgcca aagtctctct ttgccgcggc tataagctgg ggcacaaccg agagatggtt
     6421 aatgggatac ctttctgccc acttctttcc ggcaaaatgg gcgtcgataa tatatatatt
     6481 gcgcacgccg taatacttgt ataacttttc gataagattt tctgccacat tggtttctcc
     6541 cctggcaaat acttcgtctt gcatgccgta ggggaaatag gcaaaaaata catcggtagg
     6601 ggaggctttc acttctttga gcgtctgcaa aatcagttcc agttctatta aaccttcgtt
     6661 tgggctgggc gcgccagaat gcaacacaac cacatgctta cccgaaagtt tgctcgcttg
     6721 ggggattttt acgtataatt cgccgtcggg gaaatgcctg gccccttctt tatttaccgc
     6781 gggaaatatt ggggaaagac ctgacttctt tattttcttg gcaaggtgtt gtgcctgcaa
     6841 ggttgggata agaaacatag agcaattcct ttattgtagg gaaacgtgcc aaaaaaagca
     6901 aaaagtgtta ccatgatacg aatgacctgg tataatttgg ccttattctc gctttttgcc
     6961 tacggaatcc agaattttct gtacaaagtg gctgcagaaa agaaatgcaa cacggcctgg
     7021 gtaatggttg ccatgatgac cacgattacc gtacttggag ccgtattttt tgttatatcg
     7081 ggagcccaaa ttaccaatct gtcgcttttg cttttgcttt cttttacggg aggcccttta
     7141 tttttggcaa gccttgccgc ccggacagaa gccttaaaaa atgccagcac aagcgtagta
     7201 tatcccatta cgcgctttag cgttatttta gtcgtgctgt tttccattgt atatttccgc
     7261 gaacatcttt cgatgtacca aatagccggc atcggccttg cggttatcgt gtttgtactg
     7321 cttacgcggc ataatcacga agaacgcgcc cggcatgcca attttcgggc cggtgtcatt
     7381 ttagccgtta ttgcattggt aacgggagct ggcgccacca ttatcagtaa atttgccgca
     7441 gagtcgtttg aggaattttt ttcgtttatt gccatatcca acgccataaa tatttttttg
     7501 gcgctcggca taaaaaacaa gctgcagcga gaacgcgaaa atccaaagca tacaaacgca
     7561 attattatcg gcgttctttc ggggttgttt aattttgtag gctactacgc tattatgcat
     7621 gcgcttgccg cgggaccctt gagcattatt gctgccgtac acagcatgaa ctttgttatt
     7681 gcgattttgc tggcagtgct catttataaa gaagagctta ccaaaacgcg ccttgcgggc
     7741 attgggctta cggtattggc ccttatttta ttgcgacttt cctaaatgta tgactaagga
     7801 ttcctctggc gaagaacaac acgaacaatg gctcggaaag ctgcaaggaa cgaaagaata
     7861 tgcgttcgac cgccccgctt tgcacacaga agtttttggc ttgcagtttg ccaatcccat
     7921 aggtttggcc gcgggattcg acaagcacgc cacagccttg ccgcagttag catcattagg
     7981 atttggcttt ttggaggtgg ggactgtaa
//
LOCUS       DASRUP010000050         7842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_979045, whole genome shotgun sequence.
ACCESSION   DASRUP010000050 DASRUP010000000
VERSION     DASRUP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979045"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFE94_01925"
     CDS             complement(<1..548)
                     /locus_tag="VFE94_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX49890.1"
                     /translation="MRNHIYKPTTKRYSRIRPKNTRPQYGPRRNMGPKLDISAFINKE
                     LQPEQEVQAFVPEHSFGEFGLAPVISSIIEKRGYKNPTAIQDQIIPHIMQKTDVVGLA
                     NTGTGKTAAFLIPLIHKALENRGKGNGQVLVLAPTRELALQIDQEFRLFASQLSLYST
                     VCVGGESIGMQIRQLRRQNHFI"
     gene            complement(541..2343)
                     /gene="typA"
                     /locus_tag="VFE94_01930"
     CDS             complement(541..2343)
                     /gene="typA"
                     /locus_tag="VFE94_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZX49891.1"
                     /translation="MEIRNIAIIAHVDHGKTTLTDALLRQTGAASSDVSMDSNTLEQE
                     RGITIYSKNAAVLYKGTKINIVDTPGHADFGSEVERVLRSIDSVVLLVDAQEGPMPQT
                     RFVLKKSLELGLRPILAINKIDKPAANPARVHDQVFELFVELGATEEQLDFPVVYTIG
                     REGIAKLSLDEESKDLTPLLDTILKHVPNVHAQGEEVFSAQPFNLAYDNFLGRLAIVR
                     VLKGNIQDGDTVYVKKQSGDTQRYKITKLFSFEGVTRVETKEIGSGDIAIIAGLSDVY
                     IGDTVSNSPDVPLLSAITVDEPTIELTFLVNDSPFAGREGKFVTSRQLRERLEKELEV
                     NVGLKVDFDKESAEAFTVYGRGELHIAILMENMRREGYELQVSQPRVIIKEIDGKKHE
                     PFEEVVVDVPEEFQGTVIEKLSARGIQLLDMDFRENHVFLRLEGPSRGLFGYRSQFII
                     DTKGKGIMASRVLGYRPYAGDIALRKVGSMISQETGKALAFSLYNLQSRGTLYIKENT
                     EVYEGMVIGNTSKGEQMTVNPIKGKHLTNMRAAAADEAIRLVPCQLLSIETGLEVMDK
                     DEYLEVTPKSVRLRKRHLTEVGRTRAKREELAYA"
     gene            2611..3093
                     /locus_tag="VFE94_01935"
     CDS             2611..3093
                     /locus_tag="VFE94_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49892.1"
                     /translation="MHKKLFAFVLLPAVALGATAITIGTQVSAHGGGKTSISSELIGK
                     LNLDEAKVEAAIQEIRAERKAEKQEHKQEKAQTRYEEWLSEAVEDGELTEAQKQLILA
                     KRAELIAKFEAEKTALETWSSQNDIGLKYLVGGHKGLHGFGLHKGFWKFPTLGEESDD
                     "
     gene            complement(3102..4604)
                     /locus_tag="VFE94_01940"
     CDS             complement(3102..4604)
                     /locus_tag="VFE94_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZX49893.1"
                     /translation="MRFFGEFLNTFFTMYRLMLYFLGTLVVVAIVLSALGFLPYRPLD
                     IALQTIAFVALCWAANTFLARLVKTKPNFESPIITALILSLIAGPFSLPEEWLPLVVM
                     AGAAMASKYLLVRSKSHVFNPAAFGVVVSALVLQYPASWWVGSKFMLPFVLVGGLLMV
                     RRIRRFPLVLSFLGLYLGFLSLEPLLAAGSSLLQVATLAQNLLFSSPLLFFAFVMLVE
                     PLTAPQTPLARTQFGVLVGGTFFLLQRFASAIPFSLEFALLLGNAFFALTSPGFRQSF
                     VLRKKENLAPSISNFWLEPVRPFSFRAGQFLEYTLAHAHSDARGMRRYFTIASSPKET
                     QILLCSRFSEKGSSFKKALAAMQEGQEIIASKVAGDFVLPPDKTKKIAFIAGGIGVTP
                     FRSMVKYLLDTNQSRNIVLLYGAEKEGDLVFRDVFQKAQTKFGMKSVYVTEGFIDEKV
                     IVREVPDFKERIFYVSGPEPMVQALGKTLGNMGVPRKQIKRDYFPGYKAV"
     gene            complement(4604..5086)
                     /locus_tag="VFE94_01945"
     CDS             complement(4604..5086)
                     /locus_tag="VFE94_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZX49894.1"
                     /translation="MQNKIFIAIAAVAIIGVGGWWFLSQGAQKEVSQEQQQEEQQEMT
                     GLNDEANDGGNNPDREEPSEGNQGGSEEIVVRATDSGYEPSSLTVSTGTKVTFVNESS
                     RPVQTASDVHPSHLILPEFDALEGILSGQSYSFTFGKVGTWRYHNHLRPQHTGTIIVE
                     "
     gene            complement(5155..5937)
                     /locus_tag="VFE94_01950"
     CDS             complement(5155..5937)
                     /locus_tag="VFE94_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX49895.1"
                     /translation="MFERLKGVLLQEYFISVRSLEVWFDMVFFPLVTTVVFGFVAKYL
                     SGENLLAASYLLLGLLLWHVLQVSQYSMSMGVLWNVWSRNLSNMFITPLSIKEYIIAE
                     TISSLIKSLGMSLVLSFTVYVVFGFDVLQIGLLNYLLYFINLSLFAWAVGIVLVGVIF
                     KYGTRIQALAWSIIFLFQPLTAAFFPVSVLPSGIRELAFAFPPTYIFEAARGNILDAS
                     LNLQYVGIAFILNIVYFVFALAVFHWMFKKSKETGQFARNEG"
     gene            complement(5930..6856)
                     /locus_tag="VFE94_01955"
     CDS             complement(5930..6856)
                     /locus_tag="VFE94_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX49896.1"
                     /translation="MADTVLEVENLVKMYDKFKAVDGISFRVPKGKVLGLLGPNGAGK
                     TTTIQVLTGITSATSGKIKYFGKDFSRHRQHCLSRINFTPSFNTLQNRITVWQNLLVY
                     AQFYSVKNARSRILELTRYMEIDDLLHQQFLWLSAGQKTRVNLVKALINDPELILMDE
                     PTASLDPDIADKTLTFLERMKKEKNLSILYTSHDMSEVTRICDEVIFLDHGKIVAQDT
                     PLNLTKMVQATELRLVFEGVKGPVETYLKEKGQEFSYPNKNTVVIRTEEKMIPKLIFG
                     LGKAGVFIVDIEVKKPTLEDVFLQIARREKHV"
     gene            complement(6857..>7842)
                     /locus_tag="VFE94_01960"
     CDS             complement(6857..>7842)
                     /locus_tag="VFE94_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49897.1"
                     /translation="AAIIVVGAWWFWPNQGAAPQENQEQDGELEQNEAAENEGNSQEV
                     LVGAAESESASVSEISWQKDSGSRVINGSVPFVHTLKDGKVSLYYCSGDEGILSAISN
                     DGLTFAKEPGVRIAPGAGFELIVCDPTMVDLPDGRVRMYYKGANSMNPGPGQSIHKVF
                     SAISSDGLSFQKEGLRIDSETNGDNGWASVPDAITLPDGRVRIYYVTASNMEHGIGSA
                     ISSDGLNFVKEAGLRVPNLVDPALIRIGDTYLLFAASIDERFAALPKGIYYAESSDGL
                     HFGEPVAVFQENDVYDPSVLKIDENTIRVFYGKVVPPQLPAVESHTGHIAN"
BASE COUNT         2094 a         2029 c         1717 g         2002 t
ORIGIN      
        1 ataataaaat ggttttggcg ccggagctgg cggatttgca tgccaatgct ttcccctccc
       61 acgcatacgg tggaatacaa agaaagctgg ctcgcgaaca agcgaaactc ctggtcgatt
      121 tgcaaggcaa gctcgcgggt gggagctaac actaacacct gcccgtttcc tttgcccctg
      181 ttttctaatg ctttgtggat aagcggaatt aagaatgccg ctgttttccc tgtgcccgtg
      241 tttgccaaac caaccacatc tgttttttgc ataatgtggg gaataatctg gtcctggatg
      301 gcggttgggt ttttgtaccc ccgcttttca ataatgctcg aaatcacggg agccaagcca
      361 aactcgccaa aggagtgttc gggcacaaaa gcctgcactt cctgctctgg ctgcagttcc
      421 ttgttgataa acgcggaaat atccagcttg gggcccatgt tccgcctggg gccatactgc
      481 ggtctggtat ttttggggcg gatcctgctg tacctttttg ttgttggttt gtatatatga
      541 ttacgcatac gcaagttctt ctctttttgc cctcgtgcgc ccgacttctg taagatgcct
      601 tttccttagg cgcacgcttt tgggcgttac ttctaaatat tcgtctttat ccataacttc
      661 caagcctgtt tcaatcgaaa gaagctggca agggacaagg cgtattgcct cgtctgctgc
      721 tgcggcccgc atattggtaa gatgcttgcc tttaatgggg tttaccgtca tctgctcgcc
      781 tttcgatgtg tttccgatta ccatgccctc gtatacttcg gtgttctctt taatgtacag
      841 cgtgcctctg ctttgaagat tatacagaga gaaagccaaa gccttgcctg tttcctgcga
      901 aatcatggaa cccactttgc gcaaagcaat atcgcctgca taggggcggt atcccaacac
      961 gcgggaggcc ataatgcctt ttcctttggt atcgataata aactggctgc ggtacccgaa
     1021 aagtccgcgg ctcgggccct cgaggcgcaa aaacacatgg ttttcgcgaa agtccatgtc
     1081 caaaagctga atgcctctgg cgcttaactt ttcaataacc gtgccctgga attcttcggg
     1141 cacgtcaacc accacttctt cgaaaggttc gtgtttcttg ccgtcgattt ctttgataat
     1201 cacccggggc tgggaaacct gaagctcata cccttcccgg cgcatatttt ccataaggat
     1261 tgcaatatgc agttctcctc tcccatacac ggtaaaggct tcggccgact ctttatcgaa
     1321 atccacttta aggccaacgt ttacctcaag ctctttttcc aggcgctcgc gcagctgccg
     1381 gcttgtcacg aattttcctt cgcgcccggc aaacggcgaa tcgtttacca aaaaggtaag
     1441 ctcaatggtg ggttcgtcta cggtgattgc cgagagaagc ggaacatcag gggaattgga
     1501 aacggtgtcg ccaatgtata catcggacaa gcccgcaata atcgcaatat ccccggaacc
     1561 aatttctttg gtttccacgc gcgtgacgcc ttcaaaagaa aacagtttcg ttatcttgta
     1621 tctttgagta tcgccagact gcttttttac atacaccgtg tcaccgtcct gtatgtttcc
     1681 cttgagaacc cgcacaatcg ccagccttcc caaaaagtta tcgtatgcca aattaaaagg
     1741 ctgggcagaa aacacctctt ctccttgggc gtgcacattg ggcacgtgtt taagaatcgt
     1801 gtccagtaaa ggagttaaat ccttgctttc ttcgtcgagc gacaacttgg caataccctc
     1861 tctcccaatg gtgtacacta caggaaaatc gagctgctct tcggttgccc ccaattccac
     1921 aaacaattcg aatacctgat cgtgcacgcg ggcaggattg gctgcaggtt tgtcgatttt
     1981 gttgattgcc aaaatagggc gcagccccag ttcgagcgac tttttcagca caaacctggt
     2041 ttggggcatg ggcccctcct gcgcgtccac caaaagcacc accgaatcaa tagaccgcaa
     2101 cacgcgctct acttccgaac caaaatccgc gtgccccggg gtgtccacaa tgttaatctt
     2161 cgtacccttg tacaggacgg cggcattttt ggagtaaatg gtaattccgc gctcttgttc
     2221 cagcgtattt gaatccatgc ttacgtcgct tgaggctgca cccgtttggc gcagcaaagc
     2281 gtcggtaagt gtggttttgc catggtcaac atgggcaatg atggcgatgt ttcttatttc
     2341 cattacggta tttgatccca acaaaaaatc tcctcgacaa atatacaagg agaccggggc
     2401 tccgacgtaa cgtcggagca aaaacaattt caaaaaagtt ttatctcctt aaaagccgtt
     2461 tctgactttc tgctcgaatg caccaatggt agcatacctg tcaccaaatg tcaataggta
     2521 cgctcttgaa acacttttgg cgaagctgcg tctataagca cagagaggcc agtcgacaga
     2581 atacaaccct taatttccaa tatacatagt atgcataaaa agctatttgc ctttgttctc
     2641 ctgccagcag tagcgctggg agctaccgca attaccatag gcacgcaggt ttcagctcac
     2701 ggcggaggaa aaacctccat aagttcagag cttatcggga aactaaacct cgacgaagcg
     2761 aaagtcgagg cagctatcca agaaatcagg gcggaacgga aagcagaaaa acaggaacac
     2821 aaacaagaaa aagcccaaac gcgctacgaa gaatggctgt ctgaagcggt agaagacggc
     2881 gaattaaccg aagctcaaaa gcagttaatt ctggccaagc gcgcagaact tattgccaag
     2941 ttcgaggcag aaaaaactgc cctggaaacc tggtctagcc agaacgacat tggtttaaaa
     3001 taccttgttg gcgggcataa agggctacac ggttttggtt tgcacaaagg gttttggaag
     3061 ttccccacat tgggagagga atccgacgat taacaagttt actacacggc tttgtatcct
     3121 gggaaatagt ctctcttaat ttgttttcgg ggaacgccca tgttcccgag cgtttttccc
     3181 agagcctgca ccatgggttc tggccccgaa acataaaata tgcgttcttt gaaatccggc
     3241 acttctctta ctatgacttt ttcatcgata aacccctccg ttacatacac gcttttcatg
     3301 ccgaatttag tttgcgcctt ttggaacacg tcgcgaaaca ccagatcacc ttctttctct
     3361 gctccataga gcaacactat attgcgcgat tggttggtat ccagcaagta cttcaccata
     3421 ctacgaaatg gcgtaacgcc aatacccccg gctataaaag cgatcttttt ggttttgtct
     3481 ggcggcagca caaagtcacc cgcaactttg gaagcgataa tctcttggcc ctcctgcatg
     3541 gcggcaagcg ctttcttaaa actgctgcct ttttcagaaa atctgctgca aagcaaaatc
     3601 tgcgtttctt tgggagaaga agcaatggtg aagtaacgcc gcatgccccg agcgtcagaa
     3661 tgcgcgtgcg ccagtgtgta ctccaaaaac tgcccggccc tgaacgaaaa cgggcgcaca
     3721 ggctcaagcc aaaaattgct tatagaaggc gcaagatttt cctttttgcg caaaacaaac
     3781 gactggcgaa aaccggggct cgttaaggcg aagaatgcgt ttcccaaaag aagtgcgaat
     3841 tccagcgaaa acgggatggc cgaagcaaac cgttgaagca gaaaaaacgt accccccacc
     3901 aaaacgccaa actgcgtgcg cgccaagggc gtttgcggcg cagttaaagg ttccaccagc
     3961 atcacaaaag caaagaacaa aagcggcgag gaaaaaagca ggttttgcgc caaagtcgca
     4021 acctgcagca aactgctccc tgcagccagc aagggctcga gcgagagaaa cccgagatac
     4081 aaacccaaaa agcttagtac tagaggaaag cgccggattc ttcggaccat taacaaacct
     4141 cccaccaaca caaaaggcag catgaacttt gaacccaccc accaagatgc cggatattgc
     4201 aacacaagcg ccgataccac tacgccaaag gcagccgggt tgaacacgtg gctcttgctc
     4261 ctcaccagca gatactttga agccattgcg gctcctgcca taactaccag cggcagccat
     4321 tcttcgggca aagaaaacgg ccccgcaatc agagaaagaa taagggccgt aataatcgga
     4381 gactcaaaat tcggttttgt tttgacaagg cgcgccagaa aggtgtttgc cgcccagcac
     4441 aacgccacaa aggcaattgt ttgcagcgca atgtccaaag gcctataggg cagaaaaccc
     4501 agggcgctca aaacaattgc cacgacaacc agcgtgccca aaaaatagag catcagccgg
     4561 tacatcgtaa aaaacgtatt caaaaactct ccaaaaaaac gcattactca acaattatgg
     4621 ttcccgtgtg ctgcggcctt agatgattgt ggtagcgcca tgtacccact ttcccaaaag
     4681 taaaagaata actctgcccc gatagaattc cttctaaagc atcaaactcg ggcaaaatta
     4741 agtggctcgg gtgtacatcg gaagccgtct gcacggggcg ggagctttcg tttacaaaag
     4801 ttacctttgt tccagtgctt actgtcaggg agcttggttc gtatccgcta tcggtggcgc
     4861 gcaccacaat ctcttcagaa cctccctgat ttccttcaga tggctcttct ctgtctggat
     4921 tgtttccgcc gtcgtttgcc tcatcattta gccctgtcat ttcctgttgc tcctcttgct
     4981 gctgttcttg cgatacttcc ttctgcgccc cctgtgacaa aaaccaccat ccccccactc
     5041 ctataattgc aactgctgca atggcgataa aaattttgtt ttgcatacta ttgcttgttt
     5101 acgcttatta tagtgtaata acgaaatttc gagccgtttt gttttacgcc tcaattatcc
     5161 ttcatttctt gcaaactggc cagtttcttt ggattttttg aacatccaat ggaacacagc
     5221 aagcgcaaaa acaaagtata caatgtttag aataaacgca attcctacat attgcaagtt
     5281 gaggcttgca tcgagaatgt tcccgcgagc ggcctcaaat atgtaggttg gcggaaaagc
     5341 aaaagcaagc tcccgtatgc cagacggcaa cacgcttacc gggaaaaatg cggccgtaag
     5401 cggctggaat aaaaatataa tgctccacgc caaagcctga atccgtgtac catacttaaa
     5461 aatcacccct accagaacaa ttcccacagc ccacgcaaac agcgaaaggt ttataaaata
     5521 aagcaggtaa ttaagaagcc cgatttggag cacatcgaat ccaaaaacaa catacaccgt
     5581 gaaggagagc acaagcgaca tacccaacga ctttataagg gaagaaatag tttcggcaat
     5641 aatatactcc ttaatagaca gaggggtgat aaacatattg cttaagttcc tcgaccatac
     5701 attccagagc actcccatag acattgaata ttgggaaacc tgaaggacat gccacaaaag
     5761 caatccgagc aacaagtagc ttgccgccaa aaggttttcg cccgacagat acttggccac
     5821 aaacccaaag accaccgtag tcacgagcgg gaaaaaaacc atatcaaacc aaacttcaag
     5881 cgagcgcacg cttataaaat actcctgcaa taaaactcct ttcaaacgtt caaacatgtt
     5941 tttctcttct tgctatttgt aaaaacacgt cttccagcgt cggctttttt acctcgatgt
     6001 caacaataaa gacgcccgcc ttacccaacc caaaaatgag cttaggaatc attttctcct
     6061 cggttctaat aacaaccgta tttttattgg gatacgaaaa ctcctgcccc ttttctttca
     6121 ggtaggtttc tactggccct ttgacgcctt caaaaaccag ccgcaactct gtggcctgaa
     6181 ccatttttgt taaattcaag ggcgtgtcct gggctacgat tttcccgtgg tcgagaaaga
     6241 ttacctcgtc gcagattctg gtaacttcgc tcatatcgtg gcttgtgtac aaaatcgaaa
     6301 gatttttttc ttttttcatt ctctcaagaa acgtaagcgt tttgtcggca atatcggggt
     6361 cgagcgacgc cgtaggctcg tccattaaaa tcaattccgg gtcgtttatc aatgctttta
     6421 ccaggtttac tcttgttttt tggcctgccg acagccacaa aaattgctga tgcaaaaggt
     6481 cgtctatctc catatatcga gtaagctcca aaattctgct tcttgcgttc tttaccgaat
     6541 agaattgggc atacaccagc aaattctgcc aaacggtaat cctattttgc aacgtgttaa
     6601 aagaaggggt aaagtttatg cgcgacaagc aatgttggcg gtgccgggaa aaatctttgc
     6661 caaaatactt tattttcccc gacgtagccg atgttatccc tgttaacacc tgtatggtgg
     6721 tggttttgcc ggctccgttg gggcccaaaa gcccgagaac tttccctttg ggcacgcgaa
     6781 aagaaatgcc gtcgacggcc ttaaatttgt cgtacatttt tactaagttt tcaacctcaa
     6841 gaacggtgtc tgccatttaa ttggcgatgt ggccggtgtg gctttcaaca gcaggtagtt
     6901 gcggaggcac gactttccca tagaatacgc gtatggtatt ctcatcgatt ttcaaaacag
     6961 acggatcata tacgtcattt tcctggaaaa ctgctaccgg ttcgccaaaa tgaagcccgt
     7021 cagacgattc tgcgtaatag attcctttgg ggagcgcggc aaacctttca tctatagagg
     7081 ctgcaaaaag caggtatgta tcaccgattc ttatcagcgc gggatcaacc aaattgggaa
     7141 cccgaagtcc cgcctctttt acaaaattaa gaccatcgga ggatatagcc gacccaattc
     7201 cgtgctccat gtttgacgcg gttacgtaat atattcttac tcggccgtca ggcagcgtta
     7261 tcgcgtctgg gacagaagcc caaccgttgt ccccgttcgt ttctgaatct atcctcagtc
     7321 cttctttctg gaacgataaa ccatctgaag atattgcaga aaaaactttg tggatagatt
     7381 gtccgggccc ggggttcatg ctgttggcgc ctttgtagta catccttact cttccatcgg
     7441 gaagatctac catagtcgga tcgcaaacga ttaattcaaa tcctgctccc ggagcaattc
     7501 tgactcccgg ctcttttgca aacgtcaggc cgtcgttaga aatcgcagac aagatccctt
     7561 cgtcaccact gcaatagtac aagctcactt ttccatcttt caatgtatga acaaacggga
     7621 cagaaccatt gataactctt gagccgctgt ccttttgcca agatatttca gaaacagatg
     7681 cagactcgct ttctgcggcg ccaacaagca cttcctggga atttccttca ttctccgctg
     7741 cttcattctg ctctaactca ccatcctgct cttggttttc ttggggcgcg gcaccttggt
     7801 ttggccaaaa ccaccatgcg ccaactacga taatagcggc cc
//
LOCUS       DASRUP010000051        12965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_323557, whole genome shotgun sequence.
ACCESSION   DASRUP010000051 DASRUP010000000
VERSION     DASRUP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12965
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323557"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /gene="nusB"
                     /locus_tag="VFE94_01965"
     CDS             <1..280
                     /gene="nusB"
                     /locus_tag="VFE94_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZX49898.1"
                     /translation="LASGVVENMEYIDAIIEKAAPEWPIAQITVVDRNVLRLGLYELI
                     YGNKEEVPPKVAINEAIELAKNFSGESSGKFVNGVLGTVFRELEATTQ"
     gene            280..969
                     /gene="rnc"
                     /locus_tag="VFE94_01970"
     CDS             280..969
                     /gene="rnc"
                     /locus_tag="VFE94_01970"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZX49899.1"
                     /translation="MDLSSLEKRLGISFKNKELLEQAFIHRSYLNENRDFHLPHNERL
                     EFLGDAVLELVVTEYLFQEYPDKPEGELTAWRAALVNTKMLSAIGHELDFNSHLLLSH
                     GEAKEEGKARDYILANTVEAFIGSLYLDQGLKSAGKFIITHILSKLPGVLESRLFEDA
                     KSHFQELAQEKVAITPSYSVLKEWGPDHAKSFRVGVYLDKELAGEGEGLSKQEAEQAA
                     ARNALENKHWN"
     gene            974..1372
                     /gene="rpsP"
                     /locus_tag="VFE94_01975"
     CDS             974..1372
                     /gene="rpsP"
                     /locus_tag="VFE94_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZX49900.1"
                     /translation="MLTIRLFRTGKKNQPSFKIVVTDKRRSSTRGRFVEEVGFYNPLT
                     KQRNLKGDRVKYWISVGAQPSATVHNMLVADKVIEGTKIAKHKLVPAKAEAAPAEAPA
                     VAEKPAEAPQTPQEEPKAEEPKPDAQAPTA"
     gene            1669..2091
                     /locus_tag="VFE94_01980"
     CDS             1669..2091
                     /locus_tag="VFE94_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49901.1"
                     /translation="MVKNLVIAIAILSVFLVASLAALGVMTWTGIQKDGQIVALKNTL
                     EHIQLRADAEAYQTDINGDALTECRENLEKWEKNLSAYSEDLHTVSLAVAYGVVGDEI
                     RSISSYSLQEGLAWRVEAHRDYVQIQRPGSATIVPLAR"
     gene            2373..2747
                     /locus_tag="VFE94_01985"
     CDS             2373..2747
                     /locus_tag="VFE94_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49902.1"
                     /translation="MDILGMAFKKTFGFRPSRLDGMCDHEQEIVDAKLQTLAETFSYF
                     CRLQQSILEEKPARGDTLQSTERNLLKQARSLGQVNKDVTAAKRNFWRAHKLAEDRGY
                     KVMKKHTSYVPNLLQEIIKNRR"
     gene            4784..5419
                     /locus_tag="VFE94_01990"
     CDS             4784..5419
                     /locus_tag="VFE94_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49903.1"
                     /translation="MEIRNKFDIPWSKLLFAIAPFWGILGALIAIAVILNGQEAPTET
                     SAVTASDEQVERAVNNYFGANPVVGQPGQQGPAGVKGDRGEQGPVGPQGLAGVAPTAE
                     EVRDAVFDDPRFGEGLRNVIVAGQPGAQGPEGKAGFDFSKCPQDRTPSSGMASATILY
                     CNDQIIYWFATGKMISVQEAADRGHLSFHGPGWNEIRAKLKEHGIRIPTNK"
     gene            6007..6315
                     /locus_tag="VFE94_01995"
     CDS             6007..6315
                     /locus_tag="VFE94_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZX49904.1"
                     /translation="MAEEIPHYQEMLEYLVKSLVDNPNDVTVDRKVDEMGVLLTLKVN
                     AQDMGQIVGRQGSTARAIRTLVRIAGLKAHARVNLKIEEPEGGRGPRAPRESFGDLNI
                     "
     gene            6386..7033
                     /gene="trmD"
                     /locus_tag="VFE94_02000"
     CDS             6386..7033
                     /gene="trmD"
                     /locus_tag="VFE94_02000"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZX49905.1"
                     /translation="MRFDIITIFPEIFDSYFKESLLKRAQEKKLLEIRAHNLRDWSQD
                     AHKTVDDSPFGGGLGMVMKPEPIYKAVGSLKIKNSKLKVLLFTPRGKKFTQKKAAEYA
                     KLDQLIMVCGRYEGVDERVGKYIAGEQISIGDYDLMGGELPAMVVTETVARLVPGVIG
                     KEEFLKEHIKDGGFVEYPQYTRPEVFSTWKVPKVLMSGNHKKIEEWRTKHGKIIK"
     gene            7076..7158
                     /locus_tag="VFE94_02005"
     tRNA            7076..7158
                     /locus_tag="VFE94_02005"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7110..7112,aa:Tyr,seq:gta)
     gene            7166..7237
                     /locus_tag="VFE94_02010"
     tRNA            7166..7237
                     /locus_tag="VFE94_02010"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7198..7200,aa:Thr,seq:ggt)
     gene            7282..7446
                     /gene="rpmG"
                     /locus_tag="VFE94_02015"
     CDS             7282..7446
                     /gene="rpmG"
                     /locus_tag="VFE94_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZX49906.1"
                     /translation="MAAKKKPYAKFQCADCKYINYFVRKSKKATESKLALKKFCSHCK
                     KRTMHKETKK"
     gene            7469..7542
                     /locus_tag="VFE94_02020"
     tRNA            7469..7542
                     /locus_tag="VFE94_02020"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7501..7503,aa:Trp,seq:cca)
     gene            7676..8374
                     /locus_tag="VFE94_02025"
     CDS             7676..8374
                     /locus_tag="VFE94_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49907.1"
                     /translation="MFKWKPYSLVLAILALALTLLGAECEDEQNANQIAAQARALARG
                     GYLPQNDVEFQNYDDRQIIADDPSTILWCTFFPPTVGQQPFTEPIAGKLTSGDKRPYP
                     TEWRDNNSGRGYHPEKPGADGMYGHSGDYRYGFDPTRTTYDEFQDLAAFCTTAPRVWQ
                     RNETKIVVEVAQDLLQIHNEARTLLQAAKVIDDEIAVLKKETKLDVPDPEKAAKSAEL
                     KAQAMAVLSKAASK"
     gene            8388..8870
                     /locus_tag="VFE94_02030"
     CDS             8388..8870
                     /locus_tag="VFE94_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49908.1"
                     /translation="MSQQNGPEGWRNVQRTASQYNFPWGWVFRVIGIVVLLVVVIGAL
                     NFVFAWVGAGRDVASPENVQAQWRFAYDTIEDLEAIARNVCNAETALQESNNANADSN
                     TVEQRRSHLLAFQNNYERVANDYDGQVRDAFRGGLVKPPDVPERAPSLKANKLVVCSQ
                     "
     gene            9050..9604
                     /gene="def"
                     /locus_tag="VFE94_02035"
     CDS             9050..9604
                     /gene="def"
                     /locus_tag="VFE94_02035"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZX49909.1"
                     /translation="MTKLSITRYPESILKKRAAEVKRINPEVKKLILEMVEAMYEHQG
                     IGLAAPQVGISQRIIIVETSDNPRDTAKESGEHGKPLAFLNPVIAKKSKRMSPEEEGC
                     LSLPGIFVPVKRAEKVEVVCQTPTGNQVRVVAEGLMARIFQHEIDHLNGKLIIDRLPL
                     WKKLSVMRSLKSKGKTSSPANSKN"
     gene            9529..10110
                     /locus_tag="VFE94_02040"
     CDS             9529..10110
                     /locus_tag="VFE94_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HZX49910.1"
                     /translation="MEKTLRNAFAQIKREDFVPGELKELANVDQALPIGSGQTISQPY
                     VVAFMLEKLQPKPGDKILDIGAGSGWTTALLSHIVGPTGKVIGIEVLPELAEFGKTNV
                     TKYNFIEKGIATILCRNGDEGYNTQAPYDGILVSASLPQKELPKAWKEQLKVNGRIVV
                     PIQNSLWAFTKQNESNFQEKEYPGFVFVPFIPN"
     gene            10046..11140
                     /gene="mltG"
                     /locus_tag="VFE94_02045"
     CDS             10046..11140
                     /gene="mltG"
                     /locus_tag="VFE94_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZX49911.1"
                     /translation="MKATFKKKSTRGLFLYRLSRIKFLVGFTGGIALACAIFIFIQIF
                     LPLKSAAADPTPFVLEEGHGVFQVASRLKAQGLIRSRFMFSLYTIATGKYGRLQTGNY
                     LLTPALSTHEIANKFAKGDIVKETITIKEGWSLRNIAEEFENRGFFTQEEFFTVTGRP
                     GVDYRDETASSRPQDFSEEFDFLAGKPHYVSLEGYLFPDTYEVTAQETPESIVRRMLR
                     NFENNIDPVLPDIEAQGKTLFEAITMSSIIEKEVRSLEDKKLVSGILWKRLENGMRLE
                     VDSTIVYVREGNYYRVTFEELKVKSSYNTYTNYGLPPGPISNPGIESIKAALHPAKSP
                     YWFYLSPNINTTVFSETFDQHRAAANQYIR"
     gene            11280..11801
                     /gene="rplJ"
                     /locus_tag="VFE94_02050"
     CDS             11280..11801
                     /gene="rplJ"
                     /locus_tag="VFE94_02050"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZX49912.1"
                     /translation="MPKTKQQKQEILESLKENLDKQQAMVFVDYKGLKVADMSNLRKQ
                     LKQVGSRLVVAKKTLLSKVLKEKGIEADLKGMQGQIAAIFAFEDPVVPMKTTHTFGKT
                     NENLKILGGYFENEIQSANQITVIANLPTREQLLGQLVGTLAAPISGFATVLQGNIKG
                     LVVALSAMKDKKA"
     gene            11812..12234
                     /gene="rplL"
                     /locus_tag="VFE94_02055"
     CDS             11812..12234
                     /gene="rplL"
                     /locus_tag="VFE94_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZX49913.1"
                     /translation="MADEEKKEVEVPAKFKSLVESIEKLSVLELSDLVHILEDKFGVS
                     AAAPIMMGAMPAAGNGGGEQAEEKTSFNVVLTAVGGQKIEVIKVVRDATGKGLKEAKD
                     LVDATATGPQVVKEGASKEESAELKKKFDAAGATIELK"
     gene            complement(12227..>12965)
                     /locus_tag="VFE94_02060"
     CDS             complement(12227..>12965)
                     /locus_tag="VFE94_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49914.1"
                     /translation="AALILREQSRANALLQQAWQLVNGLEGEEFVALRSELEGKLAEL
                     NKIEDLQELQVVQGASLASVDVDKAIEERIAEALPSDSHAADAERFGQSVYVLESNTG
                     EIFKSSGLTAGGVSFTSWVEPTSPRKPLNAKAIAIDGNIWVLAANNEIHRYFRGSYQE
                     TIALAVFPALQNPLRIETSPQIPYLAMLEPELNRLVIIAKTGSIIKQYRAEAFKNAKD
                     LALSPDGKSVYVTDGQLVFQVFPIPSI"
BASE COUNT         3842 a         3022 c         3213 g         2888 t
ORIGIN      
        1 gcttgcctcg ggcgtggtag aaaatatgga gtatatcgac gccattatcg aaaaagcggc
       61 tcccgaatgg cctatagcgc agattaccgt tgtagaccgc aacgtgctcc gcctgggcct
      121 atacgagctt atttacggca acaaggaaga agtgccccct aaggtagcca taaacgaagc
      181 catcgaactt gccaagaatt ttagcggtga gtcttccggt aaatttgtaa acggggtgct
      241 aggcacggta ttccgtgaat tagaagctac aacacaataa tggacctatc gtcgcttgaa
      301 aaaagactgg gaatttcttt taaaaataaa gagttgctcg agcaggcgtt tattcaccga
      361 tcgtatctca acgaaaaccg agactttcac ttgccccaca acgaacgcct cgaatttctg
      421 ggagatgcag tgctcgaact tgtggtaacc gagtatttgt tccaagagta ccccgacaag
      481 ccggaaggcg agcttacagc ctggcgagcg gctctggtaa acaccaaaat gctctctgcc
      541 ataggccacg agctggactt taacagccat ttgctgctct cgcacggcga agccaaagaa
      601 gagggcaaag ccagagacta cattttggca aatacggtgg aggcctttat tggatctttg
      661 tatttagacc agggcttgaa aagtgccggg aaatttatta taacgcatat actgtcaaaa
      721 cttcccggag tgctggaatc gcggcttttc gaagacgcca aatcgcactt ccaggaactg
      781 gcacaggaaa aggtggccat aaccccttct tacagcgtgc tgaaagagtg ggggccagac
      841 cacgccaaaa gctttcgcgt tggggtgtat ctcgacaaag agcttgcggg cgaaggagaa
      901 ggattgtcta aacaagaggc ggaacaggcc gcggcaagaa acgcattaga aaacaaacat
      961 tggaactaaa gacatgctaa ccattcgatt atttagaaca ggcaagaaaa accagccatc
     1021 gtttaagatt gtggtaaccg ataaaagacg atcctccacc agaggccgtt ttgtcgagga
     1081 agtagggttt tataatccct taaccaaaca gcgcaacctc aaaggggaca gggtaaagta
     1141 ctggatttcc gtgggcgcac aaccttcggc tacggtacat aacatgcttg tggcagacaa
     1201 ggtaatagaa ggaaccaaaa ttgccaagca caagttggtt ccagccaaag cagaagcagc
     1261 gccagcagaa gctccagccg tggctgaaaa gccagcagag gctcctcaaa cgccccagga
     1321 agaacccaag gcagaggaac caaagccaga cgcacaagcg cctaccgctt aacttagcct
     1381 taaatacgcc tacttagact tattttgacc ccctctatcg ggggtcttga catttactcc
     1441 taaatatgct atattgatga gcaacgataa cagatggttt gccagtggaa ggagagagcc
     1501 accggcaata aaatctcaat ttgaagcttg aacaatagct ctctccattt ggacgagagc
     1561 tatttagtta ggtgggcaga agagtaggtt ggaaattcca aaatactctt ctcctcatca
     1621 attaggcaat agccaaatga ggaggcagca cctaaaggag gttacacaat ggttaagaac
     1681 ctggtaatag cgatagcgat attgtcggta ttcctggtag catcactggc agccctgggg
     1741 gttatgacct ggactggaat tcagaaagac ggacagatcg tagctctgaa gaacacgttg
     1801 gagcatatcc agctaagagc cgacgccgaa gcttaccaaa cggacatcaa cggcgatgct
     1861 ctcaccgagt gccgcgaaaa tctggagaaa tgggagaaga acctctccgc ctactccgag
     1921 gatctccaca ccgtctcctt ggccgtagcc tatggagttg tgggcgatga aattcgttca
     1981 atctccagct acagtcttca agaaggactg gcatggcgag tcgaggctca ccgagactat
     2041 gtccagattc aacgcccggg gagtgctacg atagtaccac tcgccaggta aaccaccacg
     2101 acacctcctc aatcaaaggc cgaaagcaga atctaattct tgcctaggcc ttcccacttg
     2161 agcccgagat cgaaaaacga tcgcggcctg aagcgggaag atacagcaca ttaagaaaat
     2221 cgtctttggc aatttgccct atagcctaga gtaaattgct gaagacgaac aatatggtga
     2281 aatattgcag tctaaagctt tcgtcgagtg ccgaagaacg gaaaagaacg ctcggcgaga
     2341 taaattgccg aagaacggca ataaggagaa acatggatat tctaggaatg gctttcaaaa
     2401 agactttcgg attccgtcca tcccggctag atgggatgtg cgaccacgag caggaaattg
     2461 tagacgctaa actccagacc cttgcagaaa ctttctccta cttctgcaga ctgcaacaga
     2521 gtatcctcga ggaaaaaccg gcgagagggg acaccttgca gagcacggag aggaatctcc
     2581 ttaagcaggc aagatctcta gggcaggtca ataaggacgt cacggcagcc aagagaaact
     2641 tttggcgagc ccacaaactt gccgaggatc ggggatacaa agttatgaag aaacacacct
     2701 cctatgttcc caatcttctc caagagatta ttaagaaccg tcggtaacta cggcgaactg
     2761 cgtggctcac cacacgcaat cagtaaggca gagggagaaa tgacgtaaaa ctcaaatctc
     2821 cctctccact cacactttag ttcagcagat tacttgccct gagcgcaagt cgaatggtga
     2881 cctgaagcgt gggatagaga accacgacag cacaaattat tttgggggag ggaaaaaagg
     2941 aaaccggtat ggcgaaatcc gtgcgggtat caacgctcgt ggggagagac gcgccaaacg
     3001 aaaaaaccca tattactacg tactatttgg atggacagaa acggcttgcc gtagtctact
     3061 taccgaatgg agagatagta gtaggggagc aattctccaa agaaataaaa cagaaaggag
     3121 gcctcgagag acaaatacaa aagtttaaat cttttattag caaaccctaa cccaacaaaa
     3181 cgaggaggaa agcccaaaaa actttccccc tcgctcccaa actttagcct gggattgcaa
     3241 aatcgcagac caaagcgtgg aaaagtaaat taaaaaggag agagttagtg tgtagactgt
     3301 aaaagattaa accgttaagg ttggaagtgg ttgtagagga gtgatccctt ctccgcaagc
     3361 acttctggtc ttaataaaaa gtaagatcat aaagcacctt gaaacaactt ccgggatggc
     3421 aatgagatta caagttttgt agtttccttg ccgttccgga aaacttaccg gacggaaaga
     3481 agtttctctg aaggagaaga attatggtag cgcaggtaaa gagttacgaa ctttggagcc
     3541 ggtacttata ctggccaacc agaaccgacc gctgcaccct tcggggtgtg gtgaacggta
     3601 tctttatcga aggggttaac ccaataatcg taactcctga atctatctac gaatatgtag
     3661 atggaatcgg gtacgttggc gggggatctt caaacgggag tgccagttgg gttcggcaat
     3721 ctgccgaaac ccggatcgtc ctcgcaggtg caggaccaga gtggcacgca tgcccacggc
     3781 accaggacac gcgggcacgg ggttggacca tctatttgtc cgaaggggcc gggatgactc
     3841 ccgcagaatt cactgaattg tgggagtcga tggaatgtcg atgcaatttc aaatgcaccg
     3901 actgcggaaa aatattttac tgcgctaatc ctactaagga aggcgccctt tgccccagac
     3961 ttcagtgttg gaacaagaag gtctgggcca cccccctaac gtaagtttct cattccttaa
     4021 ggggtcaccg ctagcgaaag ctagtaaacc ctgctgattg tcctctgcgg cctgaaaaat
     4081 tggcgcaaga tagtcggtga gtctctatgc cctggtgaag caacgaagag tttggaagtc
     4141 tcgtagtcgc gggtatagac gcaggaggga taccgaaacg gcggttaacc ctcgcgaaag
     4201 acgataccgt tctgtgcaat gcatggaaag cggccatcct ctggtcgtag tcgagccgat
     4261 accacttccg cggcaagacg aataaaagag gaagttcatt aaggaggcaa ctatgctctc
     4321 caaaacttga gggaatgtaa cgattatatc aacgcattcc ctctcctacc aaaactcacc
     4381 attcgactat gcttatggcg aagtttggtg ggagtagccc caacttgcac attacaatgt
     4441 tttggagccc gaagtgaagg caacagttcg ttatgtaact aattatttat ttgtctttac
     4501 ctcgggcttc aagccaaaat atacggaata gggaggaaaa accatgaggg ctttggaacc
     4561 aggaatcccg gtacaagtca cccccgacgg aaaaacaatg ggcttcttca atggatcctg
     4621 ggaggaggtt tacatcccgg aaactcacgc gaacggcggt agcccctccg cgaccgcgac
     4681 ggcagatgcc gaagcggttt cggaagtcac aatccacaac cactccacag cgggcaatgg
     4741 aaagaccgaa cgtgctgtgc cttctggaaa tgacaccccg accatggaaa ttaggaacaa
     4801 attcgatatc ccatggtcta aactcctatt tgccatcgca ccgttctggg gaattctggg
     4861 ggctctcata gctatagccg tgatactcaa cggccaggag gcacccacag agacctcagc
     4921 ggtaaccgct tcggacgaac aggtggaaag ggcggtaaat aattattttg gcgcaaatcc
     4981 ggttgtcgga cagcccggac agcaaggacc agcgggtgtc aagggcgatc ggggagaaca
     5041 gggtccagtt ggaccgcaag ggcttgccgg agttgctccc actgccgaag aagttcggga
     5101 tgccgtattc gatgacccgc gcttcggcga aggtttgaga aatgtgattg tggctggcca
     5161 gcctggggcg caaggacccg agggtaaagc cggtttcgac ttttcgaagt gtccccaaga
     5221 ccgaactcct tcctctggaa tggcgtccgc aaccatactt tactgtaacg atcagatcat
     5281 ctactggttc gctacaggaa agatgatatc tgtacaagaa gcagcggatc gcggacacct
     5341 aagtttccac ggtccaggct ggaacgaaat ccgtgcaaaa ctcaaagagc acggaattcg
     5401 aatccccacc aacaaataag cagcggaagc tgaagccccc gctgcccaag aacagagctg
     5461 gttgtgcata ttgcaaaccg ccagccagct ctgtacctat tataacagaa taaaaagaat
     5521 tctgtttccg ccaaagtttg taaaagatat gatattatgg attttggcgg accttaaaat
     5581 agaggagtag aaatgcggtc tgaaaccatc atatttttaa ttattgttct tgttctcatt
     5641 acaattgggt ttggcgtgac gctgctggat cagcagggtc aacaaaacca agaaaacccc
     5701 tatggtcaga tagagagctc cggctctcta tctgacccaa ttatattgag actagaagcg
     5761 ctttcgtagg agaccgtttt tgcccgcaag ggccagctct ctccacatgc gtagctacgc
     5821 tacgcgtttc tcctgcgaaa gcacttctgg tcttgcatac ccttgacaag caggaagacc
     5881 agccatacaa taagagagtc gcagaatcgc cggaatcacg acggaccgga cggaagcgac
     5941 cttccgagag gaaaacggaa aaaggtcgaa cttgttagct gttttctgat aactgtttac
     6001 tgaaatatgg cagaagagat acctcactac caggagatgt tggaatacct tgtgaagtcg
     6061 ctggtagata acccaaacga cgtaaccgta gaccgcaagg tagacgaaat gggcgtcttg
     6121 cttacgctaa aagtgaatgc ccaggacatg ggccaaatcg ttggccgcca gggttctacc
     6181 gccagagcca ttcgcacgtt ggttcgtatc gcaggcctca aagctcacgc cagagtaaac
     6241 ctcaaaatcg aggagccaga gggcggtaga ggaccacgcg cacccagaga atcttttggt
     6301 gatttgaaca tctagcgcga aatttgaact tcgagaaaac ggtgctacca taacaatatg
     6361 gcagcccgtt ttcatttaag cccctatgcg gtttgatatt ataactattt tcccggagat
     6421 ttttgactct tattttaaag agtcgctttt aaaacgcgcg caggagaaaa aacttttgga
     6481 aattcgcgcg cacaacttgc gcgactggtc ccaggacgcg cacaaaaccg tagacgacag
     6541 cccgtttggc gggggtttgg gaatggtaat gaaacccgaa cctatctata aagcagtagg
     6601 aagtttaaaa attaaaaact cgaaattaaa agttcttctt tttacgccga gggggaaaaa
     6661 gtttacgcag aaaaaggctg cagaatatgc caagcttgat caacttatta tggtttgcgg
     6721 caggtacgaa ggagtggacg aacgcgtcgg caaatatatt gcaggcgagc aaatatcgat
     6781 aggggattac gacttaatgg ggggcgaact gcctgctatg gtagttaccg aaacggtggc
     6841 ccgactggta cccggtgtta ttggaaaaga ggaattttta aaagaacaca tcaaagacgg
     6901 aggatttgtg gagtatccgc agtatacgag acctgaagta ttttccacct ggaaggtccc
     6961 caaagtatta atgtcgggca accacaaaaa gatagaggag tggagaacca agcacggaaa
     7021 gattatcaaa tagcagttga aaagtaggag attttgtgtt aggattttaa ggaacgggtc
     7081 ggtggcagag tggacaattg cacgtggctg taaaccacgc gcctcacggc tacgggggtt
     7141 cgaatccctc ccggcccact tcaaagccga gatagttcag tggcagaacg ctttcttggt
     7201 aagaaagagg tcccgggttc aattcccggt ctcggctcaa agtcagaagc aataaacaat
     7261 taacaatcga caattaatgt tatggcggca aagaaaaaac cttatgccaa attccagtgc
     7321 gcagactgca agtatattaa ctactttgta cggaagtcca aaaaggcaac cgaatcgaag
     7381 ttggcgttga aaaagttctg cagtcactgc aaaaagcgca ccatgcacaa ggaaacaaag
     7441 aaataatccg tctcatcggc gggttctggg gggtgtggtg aagtggtatc acggaggtct
     7501 ccaaaacctt cgtcgggggt tcgattccct ccacccctgc cacatattta aacgtacgta
     7561 cttgaccaag ataaaaacct tgacattaat aagcaagcgt gctattatat tgatgttcct
     7621 taacaaacga ccacttttgt ggagaaagtg atctaaacaa taagaggtgc aacgaatgtt
     7681 taagtggaaa ccctattctc tggtgctagc tattcttgct cttgccctga ctcttcttgg
     7741 tgctgaatgc gaggatgagc agaatgcaaa ccaaatagcg gcgcaagcaa gagcactagc
     7801 acgaggtggt tacctacctc aaaatgatgt tgagttccaa aactacgacg atcggcaaat
     7861 aattgctgac gatccatcca ctattctatg gtgtacattc tttccaccta cggtagggca
     7921 acaacccttc actgaaccta tcgcgggaaa gctaacgagc ggcgacaaac ggccttaccc
     7981 aacggaatgg cgagataaca atagcggcag aggctatcat ccagaaaagc ctggtgccga
     8041 tggtatgtat gggcattcgg gcgactatcg ctatgggttc gatccaacac gtacgaccta
     8101 tgatgaattc caagatctgg ccgcgttttg tacgacagcg cctagggtct ggcagagaaa
     8161 cgaaaccaaa attgttgtag aggtagcgca agatcttctg cagatccata acgaagctcg
     8221 tacactacta caagcagcaa aagtaattga tgacgaaatc gccgtcttga aaaaagaaac
     8281 caagctggac gtacctgacc cggaaaaagc agccaagtca gccgagctta aagctcaagc
     8341 catggccgta ctctcaaagg cagcatctaa atagcgagaa ggatataatg tcacaacaaa
     8401 acggaccaga aggatggaga aacgttcaac gcacggcatc ccagtacaat tttccttggg
     8461 gttgggtatt cagggttatt gggattgtag tgctactcgt ggttgtgatt ggggcactaa
     8521 acttcgtctt tgcctgggtt ggggccggcc gtgacgttgc tagccctgaa aacgtccaag
     8581 ctcaatggcg gttcgcgtac gacacgatag aagaccttga agccattgct cggaatgttt
     8641 gcaacgcaga aactgcgctc caagaatcca ataatgctaa cgcagattcc aacacagtag
     8701 agcaaagacg aagtcacctg ctggcgttcc agaataacta cgagcgagtt gccaatgact
     8761 acgatggaca ggtgcgcgac gctttccgag gcggtttagt aaagccacca gatgtcccag
     8821 aacgcgcccc gtccctcaag gcaaataaac tcgttgtctg ttcccagtaa cattttctaa
     8881 ttgggcttga tcgtaagatc tctccacagg ccctctttat agggttgacc atgtgctttt
     8941 tacagccatt ctcaacccgt tttgtttttc aatcaagggt ttcgttgatt tgatacaccg
     9001 taaccgcaag tggcagaata gcaaaaaacc tgctatatta aggttgttta tgacaaaact
     9061 ctctattaca aggtatccag aaagcattct caaaaaacgc gcagcggaag taaagcgcat
     9121 aaaccccgaa gtcaaaaagc ttattcttga aatggtagaa gccatgtatg agcatcaggg
     9181 aatagggctc gcggccccgc aagttggaat ctcccaacgc attattatcg tagaaacttc
     9241 agataaccca cgcgacacgg caaaagaatc cggggagcac ggaaaacccc ttgccttttt
     9301 gaatcctgtt atagccaaaa aatccaagag gatgagcccg gaggaagaag gctgcctaag
     9361 cctgccagga atttttgtgc ccgtaaaacg cgcagaaaaa gtagaggtgg tatgccagac
     9421 tccaacggga aatcaggttc gggttgtggc tgaaggctta atggcacgca ttttccagca
     9481 cgaaatagac catttaaacg gcaaattgat tatcgacaga ctaccgctat ggaaaaaact
     9541 ctccgtaatg cgttcgctca aatcaaaagg gaagacttcg tccccggcga actcaaagaa
     9601 ttagcaaacg tagaccaagc cttacccata gggtcggggc agacaatttc gcaaccctac
     9661 gtggttgcat ttatgctgga aaaactccaa ccaaaacctg gagacaaaat actcgatatt
     9721 ggcgcaggat cgggctggac cacagcgtta ctttcgcata ttgtggggcc aacaggaaag
     9781 gtaatcggca tagaagtatt gccggagctt gccgaatttg gaaaaacgaa cgtaaccaag
     9841 tataatttta tagaaaaggg gattgccacc atactatgtc gaaatggcga cgaagggtac
     9901 aatacacaag ccccatatga cggcatcctc gtttctgcaa gccttcctca aaaagaactg
     9961 ccaaaagcgt ggaaagaaca gctaaaggtt aacgggagaa ttgttgtgcc catacaaaac
    10021 tccctctggg cttttactaa gcaaaatgaa agcaactttc aagaaaaaga gtacccgggg
    10081 tttgtttttg taccgtttat cccgaattaa gtttctcgtc gggtttacgg gcggtatcgc
    10141 tttagcttgc gcaattttta tttttattca aatttttctt cccttgaaaa gcgccgccgc
    10201 cgacccgact ccgtttgttc ttgaggaagg gcacggcgta ttccaggttg cctcgaggct
    10261 caaagcgcaa ggacttattc gttcgcgctt tatgttttcg ctctacacca ttgcaacagg
    10321 aaaatacggc agactccaaa cagggaacta cctgttgact cccgccttaa gcacgcacga
    10381 aattgcaaac aagtttgcca aaggcgatat tgtaaaggaa acaattacca taaaagaggg
    10441 gtggagcttg cggaatatcg cagaggaatt tgaaaaccgc ggcttcttta cgcaggagga
    10501 attttttacg gtaacagggc gccccggcgt ggattaccgg gacgaaaccg cttctagccg
    10561 ccctcaagat ttttctgaag aatttgactt tcttgcgggc aaacctcact atgtaagcct
    10621 cgaaggatat ttatttcccg acacctatga agttacggcg caggaaaccc ccgaaagcat
    10681 tgtgcggcgc atgctccgca attttgaaaa caacattgat cccgttctgc cagacattga
    10741 agcccaaggc aaaaccttgt ttgaggctat caccatgtct tcgattattg aaaaagaagt
    10801 tcgcagtttg gaagataaaa aactggtatc tggaattttg tggaagcgtt tggaaaatgg
    10861 tatgcgcctt gaagtagact ctactatagt atatgtgcgc gaaggcaact attaccgggt
    10921 aacatttgag gaactaaaag ttaaatcttc gtacaatacc tataccaatt acggacttcc
    10981 tccggggccc atatccaacc cgggcataga aagcataaag gctgccttac accccgccaa
    11041 aagcccgtat tggttttatt tgtcgcccaa tataaacacc accgtgtttt cagagacctt
    11101 tgaccagcac agggcggcag ctaaccagta tattcgctag ccccttgacg aattctgtgc
    11161 tcttgtgcta agatacgcgc ataaccgtag acagcaggtt tggcctcaag gcctgtaaaa
    11221 ccctgccgag gtagcgacat ttaccttgct tttgtctgcg gagaccgcag attttttata
    11281 tgccaaaaac aaaacaacaa aaacaagaaa tactcgagag cttaaaggaa aacctcgaca
    11341 agcagcaggc aatggttttt gtggactata aaggactgaa ggtagcggat atgagcaatc
    11401 tgcgaaagca gttaaaacaa gtggggtccc gcctggtggt tgcaaagaaa accctgcttt
    11461 ctaaagtgct gaaagaaaag ggaattgaag cggatttgaa aggcatgcaa gggcagatag
    11521 ccgccatatt cgcattcgaa gatccggtgg taccaatgaa aaccacacac acatttggga
    11581 aaaccaatga aaatctgaaa attttgggtg gttattttga aaatgaaata caaagtgcaa
    11641 accagattac ggttatcgcc aatcttccca ccagagaaca gcttttgggc caactcgtgg
    11701 gaactctggc cgccccaata tccggatttg ccacggtatt gcaaggaaac atcaaaggtc
    11761 tggtagtcgc gcttagcgca atgaaggata aaaaagcata actatactaa tatggccgac
    11821 gaagaaaaaa aagaagtcga ggttcccgca aagttcaagt ctttggtaga aagcattgaa
    11881 aagctttccg tgctcgaact ttccgactta gtgcacattt tggaagacaa gttcggagta
    11941 tcggcggcag ctcccataat gatgggggcc atgccagcag ccggtaacgg cgggggagag
    12001 caagccgaag agaaaacttc gttcaacgta gttctcaccg cggtaggagg gcaaaagatc
    12061 gaagtgatta aagtggtaag agacgcaacc ggtaaagggc tgaaagaagc gaaagacttg
    12121 gtggatgcga cagccactgg cccccaggtg gtaaaagaag gagcttccaa agaggaatct
    12181 gccgaactca aaaagaagtt cgacgcagca ggagcaacca tagaacttaa atagacggaa
    12241 ttggaaaaac ctggaaaacc aattggccgt cggttacgta aaccgatttg ccgtctggcg
    12301 aaagcgcaag gtctttcgcg tttttaaatg cctctgccct gtattgtttt ataatgcttc
    12361 ctgttttggc gataataacc aatcgattca gctctggctc taacatagca aggtagggaa
    12421 tttgcgggct tgtttctata cgcaaagggt tttggagcgc agggaatacc gcaagagcaa
    12481 tagtttcctg atagctgccg cgaaagtagc ggtgaatctc gttgtttgcc gccaacaccc
    12541 aaatgtttcc atcgatggcg attgcttttg catttaaagg tttcctggga gaagttggct
    12601 ccacccacga agtaaaactc acgcccccgg cagtaaggcc ggaggacttg aagatttctc
    12661 ctgtattgga ttcaagcaca tacacactct ggccaaaccg ttcagcatcg gccgcatgcg
    12721 aatcagaggg caacgcctct gcaattcgtt cctcgatagc cttgtctacg tccacactcg
    12781 ccaaacttgc cccttgcact acctggagtt cctgcaagtc ttcaattttg ttgagttcag
    12841 cgagtttgcc ttcgagttca cttcgcaacg cgacaaattc ttctccctcc aacccgttta
    12901 ccagctgcca cgcctgctgc aacaacgcgt ttgccctgct ctgttctcgt aaaataagcg
    12961 cggcc
//
LOCUS       DASRUP010000052        13116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_763091, whole genome shotgun sequence.
ACCESSION   DASRUP010000052 DASRUP010000000
VERSION     DASRUP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13116
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763091"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..535)
                     /locus_tag="VFE94_02065"
     CDS             complement(50..535)
                     /locus_tag="VFE94_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZX49915.1"
                     /translation="MPKELWTKMLGEGERVVYEFTLGSRWRKLSIATVAAPLLAVAIT
                     LGFVVGFDTPWFAIALGLWIILTAGLFLYYEFYLPASHSYALTNKRMIIHLGWLNTHT
                     TSIEYTKITDTSIAQSFVDRILTNTGSIHVNTAGTSGHEVVLTHIENPFEVKRKLGEV
                     A"
     gene            complement(539..1990)
                     /gene="gatB"
                     /locus_tag="VFE94_02070"
     CDS             complement(539..1990)
                     /gene="gatB"
                     /locus_tag="VFE94_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZX49916.1"
                     /translation="MASYIPTIGLEVHAELKTQSKMFCSCKNDPLEKEPNVNICPICM
                     GHPGTLPVPNETAINQVVKVGLALDCTIAEYSKFDRKNYFYPDLPKGYQISQYDLPFC
                     EKGSLKVFDRDICITRIHLEEDTGRSQHPTDTDYSLVDFNRAGVPLMELVTEPDIRTA
                     EEAVEFARQLQLILRYLGVSDTDMERGQMRVEVNISLRPEGRQELGTKVEIKNLNSFR
                     VVEQSIDYEVKRQTELLEKGEKVVQETRGWDSSKQETFSQREKESAHDYRYFPEPDIP
                     PITFSKEYIESLRLELPELPQARRARFQLQYNLNDKQADLLIKDKELGEYFEHVASEL
                     ATLLPEKNLEKATELAINYITSDLQGLLAGMSVESENFKITAENFAELIHMITESKVS
                     SPAAKTLLKKMFETGADPDHVMQSEGLAQVSDSGEIEKIAKEIIAKNPKAVKDFKNGK
                     GNALQFLVGQMMAQTKGAINPQTAEELLQKELS"
     gene            complement(1960..2583)
                     /gene="tmk"
                     /locus_tag="VFE94_02075"
     CDS             complement(1960..2583)
                     /gene="tmk"
                     /locus_tag="VFE94_02075"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZX49917.1"
                     /translation="MTQTKGKFFVLEGIDGSGGETQRKRLADRFRENSIPAEVLKYPD
                     YSGPIGNLIHEYLYNKYDFSKEVQVLLYTADFLKDKQKIEQWKEEGKVIIADRYFLST
                     LAFQCLHDVPIENALEIAKLLKLPVPDLILLLDTPVKTSMERKNKEKSGNLDRHESNE
                     AFLGEVADLYRHLAENNVFAPWKVIDGEQPIEQVTDSIWQAISPQLD"
     gene            complement(2580..3119)
                     /locus_tag="VFE94_02080"
     CDS             complement(2580..3119)
                     /locus_tag="VFE94_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49918.1"
                     /translation="MNLGIAELHRLVKENKLVENLSQRELENPEGAGFDLRVGELYEI
                     SGQGFLGVTERETPNMTSIAKFEEGKTLEVALKPNTYYIIKTVESVHMPEDLLAIMTP
                     RSTLFRSGVYVFGGQVPPGYEGELNMGIYNFRQEEFRVEMGARVVHIMFSEVKGKGNM
                     YRGQWQGGRTTTKNRETQV"
     gene            complement(3116..3625)
                     /locus_tag="VFE94_02085"
     CDS             complement(3116..3625)
                     /locus_tag="VFE94_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZX49919.1"
                     /translation="MKVFIIAAMTADGFIAQHSGHLANWTSKEDKQFFIEKTKEAGVV
                     VMGLNTYKTIGRPLPDRLNIVYSKTEKDLPGVEVTEDEPKKLIENLAKRGFKQVAITG
                     GAQIYTMFMEAGLVDTIYFTVEPVLFGLGMGPFTKKLERKLKLEKTHMLNGNSVVLEY
                     KVLNDKTLL"
     gene            3640..4611
                     /locus_tag="VFE94_02090"
     CDS             3640..4611
                     /locus_tag="VFE94_02090"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016131386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZX49920.1"
                     /translation="MMHPEYQYLNLLKEVLDKGVKKVDRGTDIVLYSLFGGQTRYDLS
                     AGFPLLTTKKVYWKGVLHELYWFMSGSSNIKYLVDNNVHIWDDYPYKMYKKRADEGKF
                     PQLTKDEYVAKIAKDAGFAKEHGELPRIYGEQWRRWPTRTPGRTIDQLAWAIKELKDD
                     PDAKNLIVNSWNPEYLYTMATNEDASRFPICHNMYQLSQRDGKLSLQLYQRSADMFLG
                     VPFNIASYALLTIILAKVTGYEPGEFIHTFGDYHIYENHVEQVKEQLAREPRPFPKVT
                     IADSVKTIDDFRADLVTLEGYDPHPPIQAELTVAGGLYDPATGKMAN"
     gene            complement(4608..5180)
                     /gene="dcd"
                     /locus_tag="VFE94_02095"
     CDS             complement(4608..5180)
                     /gene="dcd"
                     /locus_tag="VFE94_02095"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZX49921.1"
                     /translation="MILSDQDIQATIEEGRVRMDPYPDFEKQLGPCSLDLHLGNVMKV
                     FKPSPYPYLDLKRKVDMESLMEEIVVGEGDPFILKPNDFALALTKEDFSLPADLMARL
                     DGRSSLGRLGLVVHSTAARFDPGWSGKAVMELGNLGKMPVILYEGMRICALTFETLSS
                     PAREAYGSSEKRKYTKQSETPSASRISQEF"
     gene            complement(5231..5653)
                     /gene="rplK"
                     /locus_tag="VFE94_02100"
     CDS             complement(5231..5653)
                     /gene="rplK"
                     /locus_tag="VFE94_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007475317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZX49922.1"
                     /translation="MAKPIKAVVKLHIAAAQATPAPPVGPALAPHGVNMGEFTKQFNE
                     ATRDKQGFKIPVDITIYNDRTFTFKLHQPPAAELIKKAVGIEKGSGTPNKSQVATISK
                     AQLREVAEKKMEDLNTDNIEQAMKTIAGTAKSMGIKVD"
     gene            complement(5655..6200)
                     /gene="nusG"
                     /locus_tag="VFE94_02105"
     CDS             complement(5655..6200)
                     /gene="nusG"
                     /locus_tag="VFE94_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZX49923.1"
                     /translation="MPKQQVVGERNWYVIHTYSGYEDAVTRNLKQRIESLGMEDKIFQ
                     VLVPKEKKIKIKNGKRKIVEEKIYPGYVLVEMIVTDDSWYVVRNTPNVTGFVGSGTTP
                     IPVSNEEIQYLQNRMGVESPHYEIEAEPGDNVKIIDGPFKDFDGRVSEIDKERGKIKV
                     LVNMFGRDTPVELDSLQIQKL"
     gene            complement(6204..6413)
                     /gene="secE"
                     /locus_tag="VFE94_02110"
     CDS             complement(6204..6413)
                     /gene="secE"
                     /locus_tag="VFE94_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZX49924.1"
                     /translation="MKITNPVAQIPGILNFFKEVKVETTRVNWPTREKTIKDTVIVIV
                     FAVVIAIFLSVFDYAFQFLLNEFVI"
     gene            complement(6501..8537)
                     /gene="gyrB"
                     /locus_tag="VFE94_02115"
     CDS             complement(6501..8537)
                     /gene="gyrB"
                     /locus_tag="VFE94_02115"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZX49925.1"
                     /translation="MASKKLNQPAKESGYGAKDIQVLEGLDPVRKRPGMYIGSTGPDG
                     LHHLVWEVLDNSLDEAMAGYAKNIEIRMLPDNRVQVSDDGRGIPVEKHPQTKKSTLET
                     VLTVLHAGGKFGSGAYKVSGGLHGVGVSVVNALSIYLKAEVSRDGATYQQEYSRGKPK
                     SPVKKTGTSKKTGTTITFEPDPEVFKEIKYDTKRILQHLRQQAYLTSGVRITFVDARE
                     KEHDTYAFYFEGGLASYVKYLTNGMAPMHENIFYVRGEKEEIIVEVAMRYTSEFEIYE
                     ESFANNIRNPEGGTHITGFRSALTRSINDYAKKNNVLKGEDTLTGDDVREGMTAVVSV
                     KIKEPQFEGQTKAKLGNPEAKTAVESVVVEALNDFFERNPQDARAIVEKSLLASKARK
                     AAKAARQTVLRKGILEGLALPGKLADCSSRDPEDSELYLVEGESAGGSAKQARDRRFQ
                     AILPLRGKILNVERARLDKILASKEIRSLVIALGAAIAEDFDIEKVRYQRIVIMTDAD
                     VDGSHIRALLLTLFYRYFKPIIERGYLYIAQPPLYSIQLGKGDIRYAYNEEEKNRIVE
                     EIKKQKGDRAKAKSAKKARETATQETEGGDEPAEQTEKVSGVNIQRYKGLGEMNPDQL
                     WTTTMNPENRMLWQVAIEDAKEADRIFDILMGDEVLPRKKFIQTYAKTVQNLDI"
     gene            complement(8519..10570)
                     /gene="pheT"
                     /locus_tag="VFE94_02120"
     CDS             complement(8519..10570)
                     /gene="pheT"
                     /locus_tag="VFE94_02120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZX49926.1"
                     /translation="MLFSYSWLQQYTKDKLPTPERCAELLNMHAFEVETVEKKGKDTV
                     LDIDVLPNRAHDCLSHVGMARELATISGKKLALPRAKDLQKQKGSLPQLSVQVQSSQL
                     VPRYTALVIEGVQIQPSPKWLKERLQALGVNSINNVVDLTNYIMLEIGQPLHAFDYDQ
                     IQKHKMLVRASGETEKVQTLDDQTLTLPKGTLVIEDGQGLIDLAGIKGGQRSSISAQT
                     KNIVLQAANFEAATIYKTKKRLKYSTQAADIYAHGIDPNLTTEALGRALYLLAEWKIG
                     GKVVQVIDLYPKKVVPATIRLHHAHLESVLGIAVPAKKVQSLLSQLGCQIREMPKQRF
                     MVWEVKPPTRRMDLQISEDLIEEIGRLYGFAKIPSSPPLVALSSPQKNQEIAWRERVR
                     DAFSSQGFYETYTYSLIGVNDLAAFRYSEQEKARLVALQNPTSADFAYMRDSLLENLV
                     KNVMLNEKKFSQIRLFEIGKVFSKDSRQLKETLMLGAVLAGESFYSAKGVIDFLFESL
                     GISGVWYDDYKATPEKSRPILWQNGKSAEIKVGSEEIGFVGEISFGISLNLKISKPVV
                     AIHIHMEKLVQLATEETEYLPVSKFPAVVRDIAVLVPPATKVAELMNTITATGQSLIR
                     DIDLFDMYEGEKEAGGKKSLAFHLVYQSDNKTLENKEVDTLHNNIIKQIEKNPLWQVR
                     N"
     gene            complement(10572..11552)
                     /gene="pheS"
                     /locus_tag="VFE94_02125"
     CDS             complement(10572..11552)
                     /gene="pheS"
                     /locus_tag="VFE94_02125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZX49927.1"
                     /translation="MDAKTLQKQALEAIEQAKSLAELETAYKAFLGKSGQLTGILRGL
                     AALPDEERKTQGKEANELRDLLEQHFAAKKREIQEQGVLASDSAKIDVTAPGKRIAKG
                     HLHPLTLIQRKTQEIFGSMGFEVVEGPEIETEWYNFDALNIPSGHPARDLWDTFWLKD
                     SDKLLRTHTSPVQARYMEKHEPPFRIIAPGRIFRYEATDASHEINFYQLEGLMVGKEV
                     SVANFKAVIEEFYRQFFERKVEIRLRPSYFPFTEPSFEIDMRWGKENWLEMMGAGMVH
                     PNVFKAVGYIPDDWQGFAFGMGLDRLAMMKYRIPDIRLLYSGDLNFLKQF"
     gene            complement(11701..>13116)
                     /locus_tag="VFE94_02130"
     CDS             complement(11701..>13116)
                     /locus_tag="VFE94_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZX49928.1"
                     /translation="ETVSTDFDQCDRLYFDEISLERVQDIYELENPKGMIVSMGGQIP
                     NNIAYKCHSAKLNILGTNPRNIDNAEDREKFSKLLDKLGIAQPAWRALSSIAEAKAFA
                     KKVKYPVLIRPSYVLSGEAMVTVFQEEELEKYLARAARVSPEYPVVISKFITHAKEIE
                     LDAVAQKGQLIASIVSEHVELAGTHSGDSFIVTPSQRIDTKTMRDITHIAEKIAKALA
                     ITGPFNIQFLAKEHEVSVIECNLRSSRSFPFVSKVHHINLAALATKAILGEKLEPANH
                     TEHLAHVGVKAPQFSFSRLKGADPLPSVDMASTGEVGCLGMSPEEAFMKAALSVDIRL
                     PRKNILVSISGDKQRFELLSRIRALADMQYTLYGTKDTVWFYRSQGIHIKQLYKMQEK
                     KEPNVGTYLQQGKIDFLISIPGEGQNPTSPSARLMRRAAVDHQIPLLTNTKVTKLFIK
                     ALRKYRNLDNLQIRPWSEYQT"
BASE COUNT         3001 a         3099 c         3130 g         3886 t
ORIGIN      
        1 actggatcga gagcgaaaaa gaggcagaaa aattagtaca aaaatttctc taggcgactt
       61 cgccgagttt gcgctttacc tcaaacgggt tttcaatatg ggtgagtact acttcatggc
      121 cagacgtgcc agcggtattt acatgaatgc tgccggtatt ggtaagaatg cggtcgacaa
      181 aggattgggc aatagaagta tcggtaatct tggtgtattc aatgctcgtg gtatgggtgt
      241 ttaaccagcc caagtgaata atcatgcgtt tgttggttaa ggcgtaagag tgggaagcgg
      301 gcaaataaaa ttcataataa agaaacaagc ccgcagttag aataatccag agtcccagag
      361 caatggcaaa ccacggcgtg tcaaacccca ccacaaagcc cagcgtaatc gcaacggcaa
      421 gcaaaggagc ggcaacagtg gcaatgctca attttcgcca tctgctgccc agggtaaatt
      481 cgtacacaac gcgctcccct tcccctagca tttttgtcca aagttctttt ggcataattt
      541 atgataactc tttttgtaaa agttcttctg ccgtttgagg atttatggct cctttggttt
      601 gcgccatcat ttgccctacc aaaaactgca gggcgttccc tttaccattt ttaaagtctt
      661 tgacagcttt gggattcttg gcaataattt cctttgcgat tttttcaatt tcgccagaat
      721 ctgaaacttg agctaaacct tcagactgca taacatggtc ggggtccgcg ccggtttcaa
      781 acattttttt aagcaaggtt tttgccgcag gtgaagatac tttgctttcg gttatcatat
      841 ggataagttc ggcaaagttt tcagctgtaa tcttaaaatt ctcgctttcc accgacatgc
      901 cggccaacaa accttgcaag tctgaagtaa tataatttat tgcgagctct gttgcttttt
      961 cgaggttttt ttccggcaac agcgtagcga gctcgcttgc tacgtgttca aaatactcgc
     1021 ccagttcttt atcttttata agcaagtctg cctgtttgtc gttgagatta tattgcagct
     1081 gaaaccgtgc gcggcgagct tggggaagct ccgggagttc aaggcggagc gattcgatat
     1141 attccttgct aaaagttatg ggagggatgt cgggctcggg aaaatagcgg tagtcgtgcg
     1201 cggattcctt ttcgcgctgg gaaaaggttt cctgttttga gctgtcccag ccgcgggttt
     1261 cttgcaccac tttttctcct ttttccaaaa gctcggtttg gcgcttaacc tcgtaatcaa
     1321 tagactgctc caccacacga aaggaattga ggtttttgat ttctactttg gtgcctagtt
     1381 cttgcctacc ttccgggcga agcgaaatat taacctctac cctcatttgt cctctttcca
     1441 tatcagtgtc agaaacacca aggtagcgaa gaataagttg tagttgacgt gcaaattcaa
     1501 ccgcttcttc tgcggttctg atatctggtt cagtgactaa ttccattaat ggcacacccg
     1561 cgcggttaaa atctaccagg gaataatcag tatcggttgg atgttgggaa cgcccggtat
     1621 cttcttccaa atgaattctc gtaatgcaaa tatcgcggtc aaatactttt aaactgccct
     1681 tttcgcaaaa cggcaagtcg tactggctaa tttgatatcc tttgggaagg tcggggtaaa
     1741 aatagttttt acggtcgaat ttggagtatt cagcaatagt gcagtctaac gccaaaccta
     1801 cttttacaac ctggttaatg gctgtttcgt ttggcaccgg caacgtgccc gggtgcccca
     1861 tgcaaatggg gcaaatgttt acgttcggct ctttctctaa agggtcgttt ttgcacgagc
     1921 aaaacatctt gctctgcgtt ttaagctccg cgtgcacctc tagtccaatt gtggggatat
     1981 agcttgccat atagaatctg ttacttgttc aatgggttgt tctccatcga taactttcca
     2041 gggcgcgaaa acgttgtttt cggcaagatg ccggtaaaga tccgccacct cgccaagaaa
     2101 agcctcattt gactcgtgcc tatccaggtt tccggatttt tccttgttct tcctctccat
     2161 agaagttttt acgggggtgt cgagcagtaa aattaggtcg ggaaccggaa gtttaagtag
     2221 tttagcaatt tcaagagcgt tttcaatagg gacatcatgt aaacactgga acgcgagcgt
     2281 agagagaaaa taacgatcgg cgataataac tttgccttct tctttccact gctcgatttt
     2341 ttgcttatct tttagaaaat ccgcagtata cagtaacacc tgcacttctt ttgaaaagtc
     2401 atacttgttg taaagatact cgtgaataag gttgccaatg ggacccgagt aatctggata
     2461 tttcagcacc tctgccggta tagagttttc gcgaaatctg tccgccagcc tttttctttg
     2521 cgtctctcct ccactgccat cgattccttc cagaacaaaa aatttccctt ttgtctgtgt
     2581 catacctgcg tctcgcggtt tttagtggtg gtgcgcccgc cctgccactg gccgcggtac
     2641 atatttccct tgccttttac ttcagaaaac ataatatgca ccacgcgagc gcccatttcg
     2701 acccgaaact cttcttgtcg gaaattatat atgcccatgt tgagttcgcc ctcgtatccg
     2761 ggcggcactt gcccgccaaa tacatatacg cccgagcgaa acagcgtgga acgcggcgtc
     2821 ataatagcca aaagatcttc tggcatatgt acgctttcca cggtttttat aatgtagtag
     2881 gtgtttggtt ttaaggctac ctcgagtgtt tttccttctt caaatttggc aatcgaggtc
     2941 atattcgggg tttcgcgttc ggtaacgccc aaaaacccct ggccggaaat ttcgtataac
     3001 tctcctaccc gcaagtcaaa ccccgcgcct tcggggtttt ccaactcgcg ttgcgacaaa
     3061 ttttcaacca gcttgttttc ttttactagt cggtgaagct ctgcaatgcc gaggttcata
     3121 ataatgtttt gtcgtttaat actttatatt ccaaaacaac cgagtttccg ttaagcatat
     3181 gcgttttttc gagcttgagt ttccgttcaa gttttttggt aaatggcccc atacccagtc
     3241 caaacagtac aggttctacc gtgaaatata tggtatcgac aaggccagcc tccataaaca
     3301 tggtgtaaat ttgggccccg cctgtgattg ccacttgctt gaaacctcgc ttagcaaggt
     3361 tttcgatgag ttttttgggt tcgtcctcgg taacttctac tccgggtaaa tctttctcgg
     3421 ttttggagta tacaatgttg agccgatcgg gtaaggggcg gccaatcgtt ttgtacgtgt
     3481 ttaaccccat tactaccacg ccggcctcct tggttttttc aataaaaaac tgcttgtctt
     3541 ctttgctggt ccagttggcc aaatgccccg agtgttgggc tataaaacca tcggccgtca
     3601 tggctgctat aatgaaaacc ttcattgtga tatagtatca taatgcatcc tgaataccaa
     3661 tatcttaatt tgctcaaaga ggttcttgat aaaggagtaa aaaaggtaga cagaggcacc
     3721 gacattgtgt tgtatagcct ttttggaggg caaacgcgat acgatttgtc tgcgggattt
     3781 cctttgctta ccaccaaaaa agtctactgg aaaggcgttt tgcacgagct atactggttt
     3841 atgtcgggca gctcgaatat taaatactta gtagacaaca atgtccatat ttgggatgac
     3901 tacccctata aaatgtacaa gaaaagagct gacgagggaa aatttcccca gcttaccaaa
     3961 gatgagtacg ttgcaaaaat agcgaaagac gcaggatttg ccaaagagca tggagaactc
     4021 ccgcgcatct atggcgaaca atggcgcaga tggcctactc gtactccagg cagaaccata
     4081 gaccagttag cctgggctat aaaggaactc aaagatgatc ccgatgccaa aaaccttatt
     4141 gtgaactcgt ggaacccgga gtacttgtat accatggcaa ctaacgaaga cgccagccgt
     4201 tttcctattt gccacaatat gtaccaactt tcccagcgtg atggcaagct ttcgctgcag
     4261 ctgtaccagc gttcggctga tatgtttttg ggtgtaccgt ttaatatcgc aagttacgcg
     4321 ctgctcacca ttattttggc caaggtaacc gggtacgagc cgggcgagtt tatccatacg
     4381 tttggcgatt accatatata cgaaaaccac gtggagcagg taaaagagca gcttgctcgt
     4441 gaaccaaggc cgtttccgaa ggtaactatt gcagactcgg taaaaaccat agacgacttt
     4501 cgcgccgatt tagtcacgct cgaagggtac gatccacatc cgcctattca ggcagaactt
     4561 accgtggccg gcggcttata cgatcctgcc accggcaaaa tggcaaatta aaattcttgg
     4621 gagatgcggc tggcgctggg ggtttcggac tgcttggtgt atttgcgttt ttctgaactg
     4681 ccataagcct cgcgggccgg ggaggagagc gtttcaaaag taagggcgca aatgcgcatg
     4741 ccttcgtata gaataacagg cattttaccc agattgccca gctccattac agccttgcca
     4801 gaccacccag ggtcgaacct ggcggctgtg gagtgcacta cgaggccaag gcgccccaaa
     4861 gaactgcggc cgtccagcct ggccattaag tctgctggca ggctaaaatc ctctttggta
     4921 agggccaaag caaaatcgtt tggttttaga ataaaagggt cgccctcccc taccacgatt
     4981 tcttccataa ggctttccat gtccactttt cgcttaaggt cgaggtaagg gtatggtgaa
     5041 ggcttaaaca ccttcattac gttgcccaag tgtaaatcca acgagcacgg ccccagctgc
     5101 ttttcaaagt ccgggtaagg gtccatacgc acgcggcctt cttcgatagt agcttgaata
     5161 tcctggtctg aaagtatcat atatgcgcat tatacaggaa agaaaaaccc gcctcaaggg
     5221 cgggtggtga ttagtctact ttgattccca tacttttggc cgtgccggcg attgttttca
     5281 ttgcttgctc aatattgtcg gtgtttaggt cttccatttt cttttcggcc acttcccgga
     5341 gctgggcctt gcttatggtg gctacctggc ttttgtttgg ggtgccggat cccttttcaa
     5401 ttcccacagc ctttttgatt aactcggctg ccggcggctg gtgaagttta aaggtaaacg
     5461 tccggtcgtt gtagatggta atgtcgacgg gaattttaaa accttgtttg tcgcgcgtgg
     5521 cttcgttgaa ttgcttggta aattcaccca tgttcactcc gtgaggggct aaagccgggc
     5581 ccacgggggg agcaggcgtt gcctgggctg ctgcgatatg aagttttact accgctttta
     5641 ttggttttgc catattataa tttttggatt tgtaatgaat ctaattctac gggggtatct
     5701 cttccaaaca tgtttaccag gaccttgatt tttccgcgtt ctttgtcgat ttcggaaacc
     5761 cggccgtcga aatccttgaa cgggccgtcg ataatcttta cgttgtcgcc aggctccgct
     5821 tcgatttcgt agtgaggaga ctccacgccc atgcggtttt gcaggtactg gatttcttcg
     5881 ttcgatacgg gaataggagt ggtgcctgat cccacaaatc ctgttacgtt gggagtgtta
     5941 cgcaccacgt accaggagtc gtcggttacg atcatttcta ccagcacata gcccggataa
     6001 atcttttctt ccacaatttt tctcttgccg tttttaattt taattttctt ttctttgggt
     6061 accagtacct ggaaaatttt gtcttccatg cccaaagact cgatcctttg ctttaggttt
     6121 cgagttacgg cgtcttcgta tccagaatag gtatgaatga cataccagtt tcgttctcct
     6181 actacttgtt gtttgggcat aggttagatt acaaattcgt tcagtagaaa ttgaaacgcg
     6241 tagtcaaaaa cgcttaaaaa aatagcgatt accacagcaa agacaattac gataaccgtg
     6301 tcttttatgg ttttttctct ggtgggccag tttacgcgcg tggtttctac ctttacttcc
     6361 ttgaagaaat tgagaatgcc tggaatttgg gcaaccggat ttgtgatttt catatgggta
     6421 tcttttctat cttaaaacgg ccccgagggc cactttgtat ggctcaagtg tatcagaaac
     6481 attccttggt gtcaagcgct ttaaatgtca agattttgca cggttttggc gtatgtctga
     6541 ataaacttct tacgcggcaa aacctcgtcg cccattaaga tgtcgaagat gcggtcggct
     6601 tctttggcgt cctcaatggc tacctgccag agcatgcggt tttccgggtt catggtggta
     6661 gtccagagct ggtcgggatt catttcgccc aaacctttgt agcgctgaat gtttactccg
     6721 ctgacttttt ccgtctgttc ggctggttcg tcgcctccct ctgtttcttg cgttgcggtt
     6781 tcgcgagctt ttttggcgct ctttgccttt gcccgatccc ctttttgctt cttgatttcc
     6841 tcgacaatgc gattcttttc ttcctcgttg taagcgtatc gaatgtcgcc cttgcctagt
     6901 tgaatgctgt acagcggggg ttgtgcaata tacaaatatc cgcgctcaat tatcggcttg
     6961 aaataccggt aaaacagggt aagcaaaagc gcccgaatgt gcgagccgtc gacatctgcg
     7021 tcggtcatta tgacaatgcg ctggtagcgg actttttcaa tgtcgaagtc ttctgcaatg
     7081 gcggctccca gcgcaataac caaagagcga atttctttgg acgccaggat tttgtcgagc
     7141 cgggcccgtt ccacgttgag gatttttccg cgcaagggca aaattgcctg aaatcgccta
     7201 tcgcgcgcct gcttggcaga ccctccggca gattctcctt ctaccaggta aagttccgaa
     7261 tcttccgggt cgcgcgaaga acagtctgcc aattttccag gcaaagccaa gccttctagt
     7321 atgcccttgc gaagaacggt ttggcgtgcg gcttttgctg cttttctggc tttcgaagca
     7381 agaagcgatt tttctacaat ggctctggcg tcttgcgggt tgcgctcaaa gaaatcgttg
     7441 agggcttcca ccactaccga ttccaccgcg gttttagctt ccgggttgcc gagctttgct
     7501 ttggtctggc cttcaaattg cggctccttg atttttaccg ataccaccgc agtcattcct
     7561 tcccgtacgt cgtcgccggt aagcgtgtct tctcctttga gcacgttgtt ctttttggcg
     7621 tagtcgttaa tggagcgcgt aagagccgag cgaaaaccgg taatatgcgt gccgccttcc
     7681 gggttgcgga tgttgttggc aaagctttct tcgtaaattt cgaattcgct tgtatagcgc
     7741 attgccacct ccacaataat ttcttctttc tcccctcgca cataaaaaat gttctcgtgc
     7801 atgggagcca tgccgttggt aaggtatttt acatacgaag ccaaacctcc ttcaaagtaa
     7861 aaggcatagg tgtcgtgttc tttttctctg gcatcgacaa atgtaatgcg cactcccgaa
     7921 gtaagatagg cttgctggcg caagtgctgc aagatgcgct tggtgtcgta cttaatttct
     7981 ttgaaaactt ccggatctgg ctcaaatgta atggttgtac ctgttttttt ggaagttccc
     8041 gttttcttta ccgggctttt tggtttccct ctgctgtatt cttgctggta cgttgctccg
     8101 tcgcgcgaaa cctcggcttt cagatatatc gacaaagcgt ttactaccga aactcctact
     8161 ccgtgcaatc cgccagaaac cttataggct ccagaaccaa atttgccacc tgcgtgcagc
     8221 actgtgagca ctgtttctaa ggtggatttt ttggtttgag ggtgtttttc tacaggaata
     8281 cctcggccgt cgtctgaaac ctgcacgcgg ttgtctggca gcatgcgaat ttcgatattt
     8341 ttggcgtatc ctgccattgc ctcgtccaaa ctgttgtcga gcacttccca aactaagtgg
     8401 tgcaggccgt cggggccggt ggaaccaatg tacatgcccg gacgcttgcg caccgggtct
     8461 aacccttcta aaacctgaat gtctttggcg ccgtatccgc tctctttcgc tggttggttt
     8521 agtttcttac ttgccataag ggattttttt cgatttgctt aataatattg ttgtgtaggg
     8581 tatcgacttc tttattttcc aatgtcttgt tgtcggattg gtacaccaaa tggaaagcaa
     8641 ggcttttttt gccgcctgcc tctttttctc cctcgtacat atcgaacagg tcaatatcgc
     8701 gaatgaggct ttgccctgtt gcggttatgg tgttcattaa ctccgcaact tttgttgcgg
     8761 gcggcaccaa tacggcaata tcgcggacaa ccgcgggaaa ctttgagaca ggcaggtatt
     8821 ctgtttcctc ggtagccagc tgcacaagtt tttccatgtg tatgtggata gccacaacgg
     8881 gctttgatat tttaaggtta agacttatgc caaaagaaat ttctcccaca aacccaattt
     8941 cttcagaacc tacctttatt tcggcagatt ttccgttttg ccacaagata ggtctgcttt
     9001 tttccggagt tgctttatag tcgtcatacc aaaccccgct tatgcctaaa ctctcgaaaa
     9061 gaaaatctat tactcctttg gcactgtaaa aactttcgcc tgccagcacg gcgcccagca
     9121 taagagtttc tttcaattgt ctgctgtctt ttgagaaaac cttcccaatt tcgaaaagcc
     9181 ggatttggct gaactttttt tcgttgagca ttacattctt aaccaaattc tccaataggc
     9241 tgtctcgcat gtaagcaaag tcggcgcttg tcgggttttg caaagccaca agccgcgctt
     9301 tttcctgttc tgaatagcgg aaggcagcaa ggtcgttgac tcctatgagg gaataggtat
     9361 aggtttcgta aaaaccctgg ctggagaaag cgtcgcgcac tctctcgcgc caggcgattt
     9421 cctggttttt ttgcggcgaa gacaacgcca ccaagggcgg agacgatggg atttttgcaa
     9481 acccgtacaa gcgcccgatt tcttcgataa ggtcttctga gatttgcaaa tccattcttc
     9541 gggtgggagg ttttacttcc caaaccataa atcgctgctt gggcatctct cttatctggc
     9601 agccaagctg cgacaataac gactgcactt tttttgcggg caccgcgatt cctaaaacag
     9661 attccaaatg cgcgtggtgc aggcgaatgg tggcgggcac tacctttttg gggtagagat
     9721 caataacttg taccactttg ccgcctattt tccattctgc aagcaaatag agggcgcggc
     9781 caagagcctc tgtggtaagg ttggggtcga tgccgtgggc gtatatgtcg gctgcctgcg
     9841 tggagtattt gagacgtttt ttggttttgt aaatggttgc agcttcaaag tttgcagcct
     9901 gcaatacgat attttttgtt tgcgcagaaa tgctgctgcg ttgccctcct ttgatgccgg
     9961 ctaagtctat taagccttgc ccgtcttcga taacaagcgt gccttttggc aaggtaagag
    10021 tttggtcatc tagcgtttgc actttttctg tttcgccaga agcgcgcacg agcattttgt
    10081 gtttttgtat ttggtcgtag tcgaatgcat gcaagggctg cccgatttcc agcataatat
    10141 aattggtaag atctaccacg ttgttaatgg aatttacgcc aagcgcttgc aagcgttctt
    10201 tgagccattt gggcgagggt tgtatttgta cgccttcgat taccaacgcg gtgtaccggg
    10261 gcacaagctg ggaagactgc acctgtacgg aaagctgcgg caaagaacct ttttgttttt
    10321 gcaagtcttt tgccctgggc agcgcgagtt ttttgccgga aattgttgca agctcgcgtg
    10381 ccatgccaac atgcgacaag caatcgtgcg cgcggtttgg cagcacgtca atatcgagta
    10441 cggtatcttt tcccttcttt tcgacagtct cgacttcaaa agcgtgcata ttcaaaagct
    10501 ctgcacatct ttctggcgtt gggagcttgt cttttgtata ttgttgcagc cagctataag
    10561 aaaataacat gttagaattg tttcaaaaaa ttcaaatctc cgctgtaaag caagcgaata
    10621 tccggtatgc ggtatttcat catggctaat cggtcgagcc ccatgccaaa agcaaagcct
    10681 tgccagtcgt cgggaatata ccctactgct ttaaacacat tggggtgtac catgccggcc
    10741 cccatcattt cgagccagtt ttccttcccc catcgcatgt cgatttcaaa agaaggttcg
    10801 gtaaaaggaa agtaactggg gcgcaagcga atttctactt tgcgctcaaa aaactggcga
    10861 tagaattctt ctataaccgc tttgaaattt gccaccgaaa cttctttgcc caccattaag
    10921 ccctcgagct ggtaaaaatt aatttcatgg ctggcgtcgg tggcttcgta gcggaaaatg
    10981 cggccgggag caataatgcg aaagggcggt tcgtgctttt ccatgtagcg agcctgtacc
    11041 ggcgaggtgt gcgtgcgcaa gagcttgtct gaatctttca accaaaaggt gtcccacaag
    11101 tcgcgggcgg ggtggcccga gggaatattg agcgcgtcga agttgtacca ttcggtttcg
    11161 atttcggggc cttctaccac ttcaaaaccc atagaaccaa aaatttcctg ggttttgcgc
    11221 tggataaggg ttaaggggtg caaatgccct ttggcaatac gcttgccggg ggcagtaacg
    11281 tctattttgg cactgtctga agccaggacc ccctgctcct ggatttcgcg ctttttggca
    11341 gcaaaatgct gctccaacaa atcgcgcagt tcgttggcct ctttcccctg ggttttgcgt
    11401 tcctcgtcgg gcagggctgc cagtccccgc agtatccctg taagctgccc ggatttgccc
    11461 aaaaatgcct tgtaagccgt ttccagctcc gcaaggctct ttgcctgctc tatggcttct
    11521 agggcctgtt tttgaagggt tttcgcgtcc atttcgagta ttggtatact cctctatttt
    11581 accaagaaac cagcccaaaa gcaacccaag ttatacccat attttaagac taagaatgga
    11641 cgtcactaat gctcacgatc gtggttatta gtgacatgca tggggttggc tccattcctg
    11701 ttatgtctgg tattctgacc aggggcggat ttggagattg tctaaattac ggtatttgcg
    11761 cagagctttg ataaacaatt tggttacttt ggtgttggtg agcaaaggaa tctggtggtc
    11821 tacggcagcc cggcgcataa ggcgcgcaga tggcgaagtt gggttttgac cttcgcccgg
    11881 aatagaaatt aaaaagtcta ttttgccctg ctgcaaatag gtgcccacat tgggctcttt
    11941 tttctcctgc attttataga gctgttttat gtgaattccc tggctgcggt aaaaccatac
    12001 cgtgtcttta gtgccgtaca gcgtgtattg catgtccgcc aaggctcgta tacgagagag
    12061 caattcaaaa cgctgcttgt cgccgctaat gctcaccaaa atattttttc tgggcaatcg
    12121 aatatccacc gaaagagcag ccttcataaa ggcttcctcg gggctcatgc caaggcatcc
    12181 tacttccccc gtggacgcca tgtctaccga tggcaaaggg tctgccccct taagccgcga
    12241 gaatgaaaat tggggagctt ttacgcccac atgcgcgagg tgctctgtgt ggttggcggg
    12301 ttcaagcttt tcgccaagaa ttgctttggt ggcaagagcc gccaggttta tgtggtgtac
    12361 ttttgataca aagggaaagc ttctgctgct gcgcaaattg cactcgataa cgcttacctc
    12421 gtgctctttt gccaaaaatt gaatgttaaa gggaccggta atagcgagag ccttggcaat
    12481 tttttctgca atgtgggtga tgtcgcgcat ggttttggta tctatgcgtt gggaaggcgt
    12541 tacgataaaa gaatccccgg aatgcgtgcc tgccagttcc acgtgttctg aaacaataga
    12601 tgctataagt tgcccttttt gggctacggc gtccagttcg atttctttgg cgtgggttat
    12661 gaatttgcta ataactaccg gatattccgg tgaaacgcgg gcagccctgg ccaaatattt
    12721 ttccagctct tcttcctgga acactgttac catggcttcg cccgaaagca cgtagcttgg
    12781 ccggattaac acgggatatt ttactttttt ggcaaacgct tttgcttccg caatggagct
    12841 taacgcgcgc caggctggtt gcgcaatgcc gagcttgtct aatagtttgg aaaacttttc
    12901 gcgatcttct gcgttgtcga tgttacgggg gttggttccc aatatgttga gtttcgcaga
    12961 atggcacttg taggcaatgt tgttggggat ttggccgccc atggatacaa tcatgccttt
    13021 ggggttttcc agctcgtata tatcctgcac gcgctctaaa gaaatttcgt caaagtacag
    13081 ccggtcgcac tggtcaaagt cggtgcttac ggtttc
//
LOCUS       DASRUP010000053         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1879935, whole genome shotgun sequence.
ACCESSION   DASRUP010000053 DASRUP010000000
VERSION     DASRUP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879935"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1269
                     /locus_tag="VFE94_02135"
     CDS             214..1269
                     /locus_tag="VFE94_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX49929.1"
                     /translation="MPEKSTLLQVAIIIILIAGLLLVVAATVGIGAIFMGSGSPSSGS
                     QEMLRIGVVQYAPVSNILTQGFKQGMEELGYVEGVHVEYVETAPTLDNALLSQNARDV
                     VAQNVDLIFATTAVGGLAALNATADIPIVWTHAAGAVANGLAANYNSSGNNSTGIEIS
                     FAELTRKKMEFLNRIDPTIETIGLLEPAARDFATLSTLPIVIEEAPKYNITLAKYPVA
                     STVDTQALAEVAQIMADIQAGAFDAYVYIPSPSVAGQSLETQIAMTKRLQVPSFWLTQ
                     DEVLKGGLFTYEYDNIAMGRQSAGQAHKILQGTAPLAIPIEFPAKISLIMNLKTAQEI
                     GLDIPEDILQIVDIAIE"
     gene            1266..2312
                     /locus_tag="VFE94_02140"
     CDS             1266..2312
                     /locus_tag="VFE94_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX49930.1"
                     /translation="MKLIPLVRIILFAAGFCALGLLAFFSSRQPTQQSAEPFVVGIIQ
                     YIDLLNPVADGFKAGMEELGYLEGRDIIYQFESSSGNKERIAQITNSYISRNVDLIYA
                     ITSVAAREALAETIKQGRGDIPIVYAHADNPVATGLAASLKSSQNNTAGVAVDLKELT
                     GKKLEFLRDINPGIRRVGVFTAAYSDPATALVLEELRSQALKFDIELVEYHLQNPPGA
                     TSTQELLQTTGQIQQGDIHALFQTPGPVANQQENVEVFIAFAKRLRIPSVFTSTPQIQ
                     QGGLFAYSHDFFSVGKQSAHIAQKVLNGTPPPEIPIEFPKDNILAINFKTAREIGIFI
                     PEPMLTLVTMEFEK"
     gene            2324..4141
                     /locus_tag="VFE94_02145"
     CDS             2324..4141
                     /locus_tag="VFE94_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZX49931.1"
                     /translation="MNIPFFPKKTSLRIKFAIPFVGIATLPLVLVTVITIQNLQSVQR
                     EQTANLSRLAAQEAAAEIEGFIELQFEVLKNIGTVHPEFAKDPVIRDILLERFLHRSS
                     SFIDIALLDQRGQEVARKNLVEVVTLQDLSDRAQTPEFQHTLQDGSYVGPFLLSAGKP
                     FFVIGREIRGIDGAFQGAVIAKVDARTLQDVVTRISFRQQDIRTYIVNQEGRVLAHPD
                     VSEVLGEQDLSFLPPVQRFIQEVQTTEITQTYKNELGETVLGTGILFNQAGRLSMQER
                     WFIMVEQPTSIALAPVRQITQFSLLILGFMLLLSFVGALFIAHSFAKPIRELQKGAAQ
                     IGLGNLDYAFRLDTNDELEDLAVELDKMRFRLRQIRSREENLSKLKSEFVSIVAHQLR
                     TPLSALKWALSLFEETKEKIPADQKDLLQKMTVSNERMIRLVNDLLDAARIEEGRFVY
                     NRTLITFEDLAKSVLATVKQKANQKKVTVTLQKPKETLPSILVDKEALGLALQNLVEN
                     AIHYTLPKGKVQITAMRSEDSSLLVSVEDTGIGIAKEEKERIFSKFFRGTNAIRLETE
                     GSGLGLFIVKNIVEAHGGKIWFESTQNKGTTFHFTVPLS"
     gene            4173..5687
                     /locus_tag="VFE94_02150"
     CDS             4173..5687
                     /locus_tag="VFE94_02150"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZX49932.1"
                     /translation="MEQEYREFLEEVLWQARKLRAEYADCRLYPQTHAEQLKVENGQI
                     SALQTSQSAGFGVRVLVQGSWGFYATPLVERGKIEEVVKRAVHSASQNAKLQQEKVVL
                     ASHEKEWPKEHVATYQTKVEINPFEIPLEQKIIALASADEAMRQASDKAFWRISEMSI
                     MKTRKLFASTEGVLADQTCIESGAAVQAHAMAKQGEDTQRRSWPDSHIYYMQGGYEMI
                     QKLNLAEQGAKMAQEAEQLLSASAAPTGKRGIILLQSMLNLHGHETTHGFEADRVLGT
                     EWTLAGGSFLTSILPQIGSFQFGSEKVNLTADSLMDGGVGTFALDDEGTPGQRVPLVK
                     EGKWVGLLTSRQTVPQLNEKIGHEYFKHSSGAMRAESYDNWPVIRMVNIILEPGDTDF
                     DELKDSVPEGTIMFGTNKSWSIDDVRRHFTFGTEIAWEKKMGKWEIRKNAKYHGDNLN
                     FWRNCQAVCNQKSFWLQGVGSCGKADPLQTMHTGHGSSPAYFTNIQVGSVGQSE"
BASE COUNT         1590 a         1485 c         1397 g         1343 t
ORIGIN      
        1 catccatctt gccgaacttc agtcgcactt ccacatcttc aacgacgcac agctaatcgt
       61 tgagcatagg gtcgtatata tgccactgcc gcaataagca cggctttctg aaaccagggg
      121 gccggtttta ttttctgtgg agaaaaagct tggatacgcc agcatacatg gtatgcttaa
      181 gtaatatata aacagtaaat tatttaaaaa actatgccag aaaaatcaac acttcttcaa
      241 gtagccatta tcataattct cattgcgggt cttctgctcg ttgttgcagc aacagttggg
      301 attggcgcga tttttatggg aagcggctct ccatcttccg gttctcaaga aatgctgcgt
      361 ataggagtgg tgcaatatgc tcctgtttca aacattctta cgcaaggatt taaacaaggt
      421 atggaggaac tcgggtatgt tgagggtgta catgtagaat atgttgaaac ggcgcccacg
      481 ctcgacaatg ctctgctttc gcaaaacgcc agagacgtgg tagctcaaaa cgttgacctt
      541 atctttgcca ccactgcggt aggggggctc gccgcactca acgcaactgc agatattcct
      601 attgtgtgga cgcatgcagc cggcgccgtg gcaaacgggc tggcggcgaa ctataattct
      661 tccggcaaca actccacggg aattgaaatt tcttttgccg agcttacccg gaaaaaaatg
      721 gaatttctca accgcatcga ccccactatt gaaacgatcg gcttactcga gcccgcggcc
      781 cgagactttg ccaccctctc caccctgcca atcgttatcg aagaagctcc caagtacaac
      841 ataaccctcg caaagtatcc ggtagccagc accgtggaca cgcaagccct tgccgaggtg
      901 gcacaaatta tggcagacat ccaagctgga gcctttgacg catatgtata tattcccagc
      961 ccctctgttg ccggccaatc tttagaaacg caaatcgcca tgaccaaacg cctgcaggtt
     1021 ccctcgttct ggcttacgca ggacgaagtg ctcaaaggcg gtttgtttac ctacgaatac
     1081 gacaacattg ccatgggaag gcagtcggca gggcaggcgc ataaaatttt gcagggtact
     1141 gcccctttgg ccattcccat agagtttccg gcaaaaattt cccttatcat gaaccttaaa
     1201 accgcacaag agatagggct cgacataccc gaagatatct tgcaaatcgt tgatattgca
     1261 attgaatgaa actcatacct ttggtgcgaa ttattctttt cgccgcgggc ttttgcgctc
     1321 tcggcctgct tgcgtttttt tcttctcgac aacctaccca gcaaagcgca gaaccctttg
     1381 tcgttggcat tatccaatat atcgacctgc tcaatcctgt ggccgatggg tttaaagccg
     1441 gcatggaaga actggggtac ctcgaagggc gcgacattat ctaccaattt gaatcttcca
     1501 gtgggaacaa agaacgtatt gcccaaatca cgaattctta tattagtcgg aatgtcgacc
     1561 ttatctacgc gataacttcg gttgccgccc gcgaagcctt ggcagaaacc ataaagcagg
     1621 ggagaggcga tattcccatt gtatatgcgc atgccgataa cccggtggcc accgggctgg
     1681 cggcaagcct caaatcatct caaaacaata ccgccggcgt tgccgtggac ttaaaagaac
     1741 tcaccggcaa aaagctggaa tttctccgtg acataaaccc cggcatacgg cgcgtcggag
     1801 ttttcaccgc cgcatactct gatcctgcca ccgctctggt cctcgaggaa ctccgcagcc
     1861 aggctctaaa attcgacatt gaacttgtcg aatatcacct tcaaaaccct ccgggagcaa
     1921 cttccactca agaactcctg caaaccacag gccaaatcca acagggcgac attcatgcgc
     1981 tgttccaaac gcctggaccg gttgcaaacc agcaggaaaa cgtggaggta tttattgctt
     2041 tcgcaaagcg ccttcgcatt cccagcgttt ttacttcaac gccgcagata caacaaggcg
     2101 ggcttttcgc ctatagccac gacttcttct ccgtaggaaa acaatccgcc catattgcgc
     2161 aaaaagttct taacggcacg cctccgcccg aaatacccat tgaatttccc aaagacaata
     2221 tcttggctat taactttaag acggcgcgcg aaataggaat tttcattccc gaacccatgc
     2281 ttaccctggt aactatggaa ttcgagaagt aaccattttc cgcatgaata ttccgttctt
     2341 cccaaaaaag acttcgctgc gcatcaagtt tgccattccc ttcgtgggca tcgcgactct
     2401 gccccttgtg ttggtaacgg ttattactat ccaaaacctc cagtccgtac agcgcgaaca
     2461 aacagcaaat ttaagccgtt tagcggcgca ggaggcggca gcagaaatcg agggcttcat
     2521 tgaactccag tttgaagtgc tcaaaaatat cggcacggta cacccggagt tcgccaaaga
     2581 tcccgtaatc cgagatattt tattggagcg atttttgcat cgttcgagtt cttttattga
     2641 tatcgcgctt ctcgaccagc gcggccagga ggtggcaaga aaaaatttgg tcgaggtggt
     2701 aaccttacaa gacctctctg accgcgccca aaccccggaa ttccagcata ctcttcaaga
     2761 tggatcttac gtggggccct ttttgctttc tgcgggaaaa ccgttttttg ttatcggcag
     2821 ggaaatccgc ggaatagacg gcgcgttcca gggagcggtg atagcaaaag tagacgcaag
     2881 gaccttacaa gacgtagtaa cgagaatctc ttttcgccag caagacatcc ggacatacat
     2941 cgtaaaccag gaaggcagag tgctcgctca cccggatgtt tctgaagtgc tcggggaaca
     3001 ggacctctct ttccttcccc cggtgcagag atttatacag gaagtgcaaa ctaccgaaat
     3061 tacccaaacg tataagaatg aactcggaga aacggtattg ggaaccggca ttctttttaa
     3121 tcaggcaggg cggctttcta tgcaggaacg ctggtttatt atggtggagc agccaacctc
     3181 cattgctttg gctccggtgc ggcaaatcac ccaattcagc cttctgattt tgggctttat
     3241 gctgttgctg tcgtttgtgg gcgccctttt cattgcgcat tcttttgcaa agcctatccg
     3301 ggaactgcaa aaaggagccg cgcaaatcgg gcttggcaac ctggactacg ctttccgctt
     3361 ggatactaac gacgaactcg aagacctcgc ggttgagctc gacaaaatga ggtttcgcct
     3421 taggcagata cgttcccgtg aggaaaatct ttcaaagctt aagtcggaat ttgtgtctat
     3481 tgtggctcat caacttcgaa cgcccctctc cgctttgaaa tgggctcttt cgcttttcga
     3541 agaaacaaag gaaaaaatcc cggcagatca aaaagatctg ctccagaaaa tgaccgtgag
     3601 caacgaacgc atgatccgcc tggtaaacga cctgctggac gccgcccgca tcgaggaagg
     3661 acgatttgtg tacaaccgca ccttgattac atttgaagac ttagccaaat ccgtgcttgc
     3721 cacggtaaag caaaaggcta accagaaaaa ggtgaccgtt accctgcaaa agcccaaaga
     3781 aacgcttcct tccatactcg tagacaaaga agcgcttggc ctggctttgc aaaatctggt
     3841 ggaaaacgcc attcactaca cactgcccaa aggcaaagtg cagataaccg caatgcgatc
     3901 ggaagattcg tcgcttttgg tttctgttga agacacgggc attggcatag ccaaagaaga
     3961 aaaagaacgc atctttagca aattctttcg cggcaccaac gccatccgtt tggaaacgga
     4021 aggctccggc ctcggtttgt ttattgtaaa aaacatcgtg gaagcacacg gaggcaaaat
     4081 ctggtttgaa tccacgcaga acaaagggac tacgtttcat tttactgttc cgcttagttg
     4141 aataatcccg aaggatttgt aatactcaat agatggaaca agagtatcgc gaatttttgg
     4201 aggaggtgct gtggcaggca cgaaagctgc gggcagagta cgccgattgc agactgtatc
     4261 cccaaacgca tgcagaacag ctcaaagtgg aaaacgggca aatctcggct ttgcaaacct
     4321 cccaaagcgc cggttttggc gtgcgggtat tggtgcaggg ttcctgggga ttttacgcaa
     4381 ctccgttggt ggaacgcggc aaaatcgagg aagtggtaaa acgcgcggtg cattcggctt
     4441 ctcaaaacgc caaactgcag caagaaaaag ttgtgctggc ttctcatgag aaagaatggc
     4501 ctaaagagca tgtggccacc taccaaacga aagtggagat caatcctttt gagatccccc
     4561 tggaacaaaa gataatcgca ttggcctcgg cagacgaagc catgcgccag gcatccgaca
     4621 aagcgttttg gcggatttcc gaaatgtcta ttatgaaaac ccgaaagctt tttgcctcaa
     4681 cggaaggcgt gcttgccgac caaacatgta tagagtcggg agctgcagtg caggcgcacg
     4741 ccatggcaaa gcaaggggag gacacccaac gcaggtcgtg gccagactcg catatctact
     4801 acatgcaggg gggatacgaa atgatacaaa agcttaattt ggcagaacag ggggcaaaaa
     4861 tggcccaaga agcagaacag cttctttctg ccagcgctgc tcccacaggg aaaaggggaa
     4921 ttatcctatt acagtcgatg ctcaacctgc acggccacga gaccacgcac ggctttgaag
     4981 cagaccgcgt gctgggcacc gagtggactt tagcaggcgg cagttttctt acttctatat
     5041 tgcctcaaat aggcagcttc cagttcggtt cagaaaaagt aaatcttacc gcagacagtt
     5101 taatggacgg gggagtgggc actttcgctt tggacgacga aggaactcca ggtcaacgcg
     5161 tgcctttggt aaaagaagga aaatgggtag gcctgctcac ttcgcgccaa accgtgcccc
     5221 agctaaacga aaaaatcggg cacgagtatt tcaaacattc ttcgggagcc atgcgggctg
     5281 aatcttacga caactggcct gtcatacgca tggtgaatat tattttagaa cctggcgata
     5341 ccgattttga cgaactcaag gattcggtac ccgaaggcac tataatgttc ggcaccaaca
     5401 aaagctggag tatagacgac gtgcgccggc actttacttt tggtacagaa atcgcatggg
     5461 agaaaaaaat ggggaagtgg gaaattcgca aaaatgccaa ataccacggc gacaacttaa
     5521 acttctggcg caactgccaa gccgtgtgca accaaaaaag tttttggttg cagggcgtag
     5581 gatcctgcgg caaggccgat cctttgcaaa ccatgcacac gggccacggc tcctcgcccg
     5641 cgtattttac caacatccag gtcggctccg tcggtcaaag tgaataaccc tatcccatga
     5701 atactaaaga aaccgcacaa caaattataa agattgccaa ggagcacggg gcggagtttg
     5761 ccgagattgt agtatcgcaa aatgagttct cgttaaaacg catacgagcc aacca
//
LOCUS       DASRUP010000054         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1295168, whole genome shotgun sequence.
ACCESSION   DASRUP010000054 DASRUP010000000
VERSION     DASRUP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295168"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..979
                     /locus_tag="VFE94_02155"
     CDS             179..979
                     /locus_tag="VFE94_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZX49933.1"
                     /translation="MTRYFSILFAVTLLVPSVGFAQHEGMSHGAATFSLTPDRGGYNP
                     GESFSVRVTLNAPEPVTSVQASLLYDASRLTLVSIAEEKANFPYWWGNGFSPASPTSG
                     VAMLQASSPAPGVQGSAVPVATITFYAKESGLAALVASQNSLVLNSGDTNVLDVAKPA
                     GAIFTIGSLLMPAPSSAVSSFNRNLQRGNQGADVKALQQFLNAQGFQLASVGAGSPGQ
                     ETEYFGPATQAALIRFQEMHASQILAPLGLQSGTGYFGASTRAYAQSL"
     gene            983..1582
                     /locus_tag="VFE94_02160"
     CDS             983..1582
                     /locus_tag="VFE94_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZX49934.1"
                     /translation="MKSVILTGLGVLLAYGSVALAHEGAHEQARITVSPISVDYEQGE
                     EIPVEVRISTIDPVTSVQIFLRYDPAKVQVQEITKNEEAFPFWWAQKAAEGTIELAAS
                     SPVREGVQGEVVLATVHLVGKGHANELLTVDASSVTLNADSENLLAPQSAPEEQGESF
                     EQQITPAGNRNIIFLALTVFASVGAGVATFLFVRQKKKV"
     gene            1600..>2688
                     /locus_tag="VFE94_02165"
     CDS             1600..>2688
                     /locus_tag="VFE94_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZX49935.1"
                     /translation="MRKRFFIALGLMFVVTVVFALRLLNDSLPGRFGSSDSLLSQVTP
                     GPNEALYFVSPSSGSHNVGNTFQVELRLASSAGVTSMKAYLNFNSSLLQVTNVSTTGG
                     AFQTYWEATSTSNQVRLQGSSPTPGVSGNNNLVATITFQALAAGTASLTYDASSLALK
                     SDDTNILNIGGSQTGSYTLVSDTTAPSATISINSGASSTSTTSVTLSLTCTDTSGVKD
                     VRYSNDGVFDTEVFEAFAASKAWSLSGGDGTKTVSYQCRDNSLNQNIATVSDTIVFDT
                     TAPSVPSGISYSPNPNKTGTHTVSWNASSDAGSGVAAYDVRRSLNGGGSWTTVASGLA
                     GTNFVQSPAVGAGNYIYQARAVDNAGNQS"
BASE COUNT          613 a          677 c          735 g          663 t
ORIGIN      
        1 cgtttcccgg ttgattaact attggggcaa gagtacttct ggcgatcttc aggcagcaga
       61 tattaacggc ccggcccgta cgtcagacaa caaaatcgac atttacgacg ccaacaagct
      121 catggcaaac tggacggggt agtaaaaaga taataaaata agtaataatc agcaacatat
      181 gacacgatat ttttctatac tttttgcggt aacgcttctc gttccttctg tgggtttcgc
      241 gcagcacgag gggatgtcgc atggagcggc aacattttct cttacgccag atcggggggg
      301 gtataacccg ggcgagagtt tttcggtgcg ggtaacgctt aatgcgccag aaccggttac
      361 ttcggtgcag gcatcgcttt tatacgatgc ttcccggctt actcttgtga gtatcgcaga
      421 agaaaaagca aactttccct actggtgggg gaacgggttt tcgcccgcat cgccaacgtc
      481 tggagtggcc atgcttcagg catcctcgcc ggctcccgga gtgcagggga gcgctgttcc
      541 tgtggcaacg attacctttt atgccaaaga gagcggattg gcggcactgg tagcaagtca
      601 aaactctttg gtgcttaact ccggcgatac caatgtgctg gatgtggcaa aacccgcagg
      661 agccatcttt accataggtt cattgctgat gcctgcgcct tcttctgcag ttagttcctt
      721 taatcggaac ctccaaaggg ggaaccaggg agcagacgtg aaagcgttgc aacagttttt
      781 gaacgcgcag ggtttccagc tggcctctgt tggtgctggc tctccgggcc aggagactga
      841 atattttggc cctgccaccc aagccgcgct cattcggttc caggaaatgc acgcctcgca
      901 aattcttgct cccttggggc tgcagagcgg aacaggatac tttggggcga gtacgcgagc
      961 gtacgcgcag agtctgtagc atatgaaatc tgttatactt acaggtctgg gagttttgtt
     1021 ggcatatggt tctgttgctt tggcgcatga gggcgctcac gagcaggccc gtattactgt
     1081 ttcgcccata tccgtggact acgaacaagg cgaggagata cctgtggagg taaggattag
     1141 tactatagat cctgtcactt ctgtgcagat tttccttcgc tacgaccccg caaaagtcca
     1201 ggtgcaggaa attacgaaga acgaggaggc atttcctttc tggtgggcgc agaaagcagc
     1261 ggagggaacg attgaattgg ccgcttcttc tcctgtgaga gagggggttc agggagaagt
     1321 tgttcttgct acggtgcatc tggttggaaa ggggcatgca aacgagttgc tcactgtcga
     1381 cgcttcgtct gttactttga atgcagacag tgagaacctt ctggctccgc aaagcgctcc
     1441 agaggaacag ggagaatctt ttgagcagca aataaccccg gcaggaaatc ggaacattat
     1501 ttttctcgcg ttgaccgtgt ttgcttctgt tggcgcgggg gtggcgacat tcctctttgt
     1561 aaggcagaaa aagaaggtat agctgcctgg taacctttta tgcgaaaacg attttttatt
     1621 gcattaggcc tgatgtttgt tgttaccgtt gtttttgcat tacgtctatt gaacgattcc
     1681 ttgccgggaa ggttcgggtc ttcagattcc ctgctttccc aggttactcc ggggcccaac
     1741 gaagccttgt atttcgtgtc gccttcgtcg ggcagccata acgtaggaaa tacgtttcag
     1801 gtagagctgc ggcttgcctc gtctgcgggc gtaacctcta tgaaagcata tttgaatttt
     1861 aattcgtcgt tgctgcaggt aaccaacgta agcacgacag gcggggcttt tcaaacatac
     1921 tgggaggcaa cctctacaag caaccaggtg cgcctgcaag gttcttcgcc aacccctggc
     1981 gttagcggca acaacaacct ggtggcaact attacattcc aggctttggc ggctggtacg
     2041 gcaagcctta cctacgacgc gagctctctg gctttgaaat cagacgatac caatattttg
     2101 aacatcggcg gttctcaaac aggaagctac accttggttt cagacaccac cgccccctcc
     2161 gccaccatca gcattaattc gggcgcctcc tccacttcaa cgaccagcgt taccctttct
     2221 ctcacctgca ccgatacctc gggcgtcaaa gacgtgcgtt attccaacga cggcgtgttc
     2281 gacacggaag tctttgaggc ctttgccgcg agcaaggcgt ggagcctttc tgggggagac
     2341 ggcaccaaga cggtaagtta tcagtgccgg gataacagcc tcaaccaaaa tatcgccacg
     2401 gtttcagata ccattgtgtt tgacaccact gctccttcgg ttcccagcgg aatttcgtac
     2461 tcgccaaatc ccaacaaaac ggggacgcac acggtaagct ggaacgcttc ttcggatgcg
     2521 ggttctggag tggcagccta cgacgtacgg cgttctttaa atgggggagg gtcgtggaca
     2581 acagtggcaa gcggccttgc aggaacaaac tttgtgcaaa gcccggctgt cggggctggc
     2641 aactatatct accaggcgcg cgcagtggac aacgccggca atcaaagc
//
LOCUS       DASRUP010000055         9843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_179735, whole genome shotgun sequence.
ACCESSION   DASRUP010000055 DASRUP010000000
VERSION     DASRUP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179735"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VFE94_02170"
     CDS             <1..1418
                     /locus_tag="VFE94_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49936.1"
                     /translation="ILREFLRKFPLPYRDEHTHLTGSLTPEFIYPRLMPLLEGPQKNL
                     YEIGLKEVYGGQCLPISSPEDVRELIQIKEGDRLQRYLETLYLTKLILRDRQTHRQAA
                     YHMAKKMYENCHVGNIRLKFTFSRVSKMKAESIPGLENATPENAILGLYEGFEDFKKE
                     HPSFGFVLSPSFKKEEGFYDSDSFATKEEDFMNQVDTLLSLLDKYPELQTCVTDIDTV
                     GNDTEMYRKAHFFPMQRGMRKLQQYGFHVRSHHGETWHTLNHGIQAVDNAMNIWHIDS
                     LEHGLSLGINPNFYLHTVFENVREQNMEGIKLDPASPEGKEIVAMEWGKYGDIFKKLL
                     AGELLTDADVTHFASVKFATAIEVEHYQHDVLNRMIDQEIGLTSLPSSNRKLSGTITD
                     FRNHPFSWWEKKGVTQAIGTDNYVTLNTDYLREMMILLLSDPKGLKITKLLMVATGEN
                     RRPYLSKTLWDMRKQLKKEFA"
     gene            1420..2946
                     /gene="guaA"
                     /locus_tag="VFE94_02175"
     CDS             1420..2946
                     /gene="guaA"
                     /locus_tag="VFE94_02175"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZX49937.1"
                     /translation="MIYVFDFGSQYSQLIARRIRALGCEARLVSHQYPLQKLTDAKGI
                     ILSGGPDSVYEKEAASVSKKIFDLGVPVLGICYGMQLTCHVLGGKVQRSAKHEYGPAK
                     LEIVRKSALFEKVPKSSAVWMSHGDQVATLPKGFVQTGASSNTPNAAMEQTAKDIYGI
                     QFHPEVEHTEYGSQILKNFVLNICKCKPANKLAEFIEQKVAEIQKTVGKEHVICATSG
                     GVDSSVVAALVGKAIGNQLTCIFVNSGTLRKEEDKDALKLLQDLGLNVQYVNAEKQFL
                     RALKGVTYGEAKRKIIGKEFIAVFEKSAKQIRPTPAFLAQGTIHSDVVESAKTATGKK
                     TQTIKSHHNVGGLPKKLNLKLLEPLRDLFKDEVREVGQKLGLPEHAVWRQPFPGPGLA
                     VRVVGEVTKQKLDIAREADAIVTEEIEAAGLARSFYQYFAILLNDKTVGVTGDQRKYA
                     YPVVIKAVVSSDIMTSDWARLPYDLLARISTRITSEVPQIARVLYDITPKPPGTTEWE
                     "
     gene            3109..4422
                     /locus_tag="VFE94_02180"
     CDS             3109..4422
                     /locus_tag="VFE94_02180"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZX49938.1"
                     /translation="MVAIAVIGGQWGDEGKGKVIDFLCENASVVARGTGGNNAGHTVW
                     VGKEKRVSHLVPSAGFWPGKRVILGNGMVINPDVLIEELSWLPYLNGNVPENERTQIF
                     VSPQAHVIMPYHITLDALQEAKRGADSIGTTGQGIGPAYEDKMRREGVRIGDLLNLEE
                     LLPRVASLVQAKNELIEKVYEGEPLDVDEVQSRVVSWARRLLPSIRVEYHISSLLQEA
                     VQTGKLLLEGAQGTLLDIDHGGYPHVTSSNSSIGGMLVGIGLTPKSLNHILGVFKAYA
                     TRVGSGVFPTEILDERADKIRDAGEAPEYGSTTNRPRRIGWFDMAAARYSIELNGIDS
                     IALTRLDTLDGFEKIRVCMRYTYENAHVSEYRYIPDDKTLTELRPNYGDLTGWDGVAG
                     VTKWNKLPKTAQSYVRFLEDNLGVPVSIISTGPERSSTIVRHQIF"
     gene            complement(4480..4779)
                     /locus_tag="VFE94_02185"
     CDS             complement(4480..4779)
                     /locus_tag="VFE94_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HZX49939.1"
                     /translation="MDNQQQPQQRQIQIKAQDEDLKGKYSNLMQITHTQEEFVLDFFM
                     VIPPQGTLASRVVLSPGHLKRMLAALQDNVEKYETKFGKIQQAESPEPPIGFSAQ"
     gene            complement(5175..5747)
                     /locus_tag="VFE94_02190"
     CDS             complement(5175..5747)
                     /locus_tag="VFE94_02190"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HZX49940.1"
                     /translation="MNKKFFFVFLAGIVIFSIVVRLLPHAPNFTPIAALALFAGVYAS
                     QVSKWYLFTPLGAMLVSDLFVGFYEPKTMAVVYASFFAIALIGLLARMFVFEAKLQTL
                     GLAAVSGSLLFYLTTNFAVWLFSGMYASTFSGLMLSYTMALPFLKFTLLGDLFYTALF
                     FGTYEFATAFLTKKKGRHKAAFDRVTAFRS"
     gene            complement(5750..6277)
                     /locus_tag="VFE94_02195"
     CDS             complement(5750..6277)
                     /locus_tag="VFE94_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZX49941.1"
                     /translation="MIVVFTGNGKGKTTASLGQMMRIVGQDRKAIMFQFIKGPWISGE
                     DKFLQKHLQFEGKWEIHKGGKGFVGILGDTLPFEEHKKAAEETLEHAKKAIESHLWDL
                     IVLDEVNVATSLKLLAVKDVLAILKDLPMHEFVILSGRDAPQEFIDRADLATEMRELK
                     HPYNQGELALKGVEF"
     gene            complement(6279..8663)
                     /locus_tag="VFE94_02200"
     CDS             complement(6279..8663)
                     /locus_tag="VFE94_02200"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HZX49942.1"
                     /translation="MRVSQEQKDQTNSTFSENALKMMQKRYLMLNDEGSQETPADMFE
                     RVANALAQVEKDYGKPEELIEQTARDFFAVMASKEYTPAGRTLTNAGGETPLIANCIV
                     LRIEDSMESIFQTLKEAALLQQAGCGLGFDLSEMRPANSPTKKSRGRASGPVTFLSVY
                     DKAFGTIKQQGRHGANMAMIRVDHPDVLDFIECKKVEGDIRNFNISVTVTDEFMQQLE
                     ENPRQLWYCQFRGQKMKPHQVLRYPNGAVYGSEEIDITVGELFNKLVEHAWLNGEPGI
                     AFIDTINRTNPLPGLGPIAASNPCGEQFLHAYDNCNLGSINLAQFVADGKINWERLGF
                     VTRTAVRLMDNVIDKFDFPVPQVTELAKKNRRIGLGIMGFGDMLYQLGIRYDSPEGMQ
                     AAEQVMGFINKESHLASEELAREKGEFPNLDKSILPAAGIKRRNAALTTVAPTGSISM
                     MFDTSSGVEPNFALAYVKQDKDGQQYRYFNKYFQQALDSLQLSEEQQKEIMDQVMAKG
                     SIQDVHWLPEEIRDTFMVAMDISGQAHMEMQAAFQRHVDNSISKTINFPNSATREEVA
                     QSFVAAWKLGCKSATVYRDGSRTIQILNVGSGDNMVSTTEKPSERPSASPQQGQRGEV
                     VPRKRPEIMAGKTYRMKTGYGNLYITINNDENEYPFEIFATIGKSGGFFQEQSEAICR
                     LTSLALRAGIRVEEVISDLKGIRGPMPIFTEKGTVLSLPDAIGRVLEEHVTGVKQVED
                     ILERPEKQEVFAFAKETQSMADFGFMPGCPDCGAQLVMSEGCISCKACGFSRCM"
     gene            complement(8685..9149)
                     /gene="nrdR"
                     /locus_tag="VFE94_02205"
     CDS             complement(8685..9149)
                     /gene="nrdR"
                     /locus_tag="VFE94_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZX49943.1"
                     /translation="MQCPICRIDTKVTNSRDADHGRAVKRRRLCASCGRRFTTYERLE
                     LLGVEVLKRNGAKEPFSRHKLEAGIRKALEKRPFGEEHLQKLVSSIENDIFNLDKDVV
                     DSGTIGQAVLAHLKDLDKVAYLRFASVYRNFRSPKAFEKEIQKLQQPSPHKS"
     gene            9395..>9843
                     /locus_tag="VFE94_02210"
     CDS             9395..>9843
                     /locus_tag="VFE94_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49944.1"
                     /translation="MNQIQQSVAAAILVPILSIMPALDAHTADDEDPSPIGERLVMSQ
                     TNTLLPIASPMGPERVAQTMRLYVTGYSSTPEQTDDTPFITASGKHVRDGIVATNLLP
                     LGTKIKIPELYGNKVFVVEDRMNTRLQNHVDIWFAEYWDAKNFGIKRA"
BASE COUNT         2605 a         2407 c         2330 g         2501 t
ORIGIN      
        1 acatactgcg cgagtttttg cgcaaatttc cccttcccta tcgcgacgaa catacccatc
       61 tcaccggctc ccttacgccg gagtttattt atccaaggct tatgcccctt ttggaagggc
      121 cgcaaaaaaa tctttacgaa ataggcctca aagaagtata cggcggccag tgccttccga
      181 tttcttcgcc cgaagatgtc cgggaactca ttcaaatcaa agaaggcgac cgattgcagc
      241 ggtacttgga aacactgtac cttaccaaac ttatcctgcg cgatcgccaa actcaccgcc
      301 aagccgctta ccatatggca aagaaaatgt acgaaaactg ccatgtggga aacatacgcc
      361 ttaaatttac gttctctcgc gtgtctaaaa tgaaggccga aagcattcca ggccttgaga
      421 acgccacgcc agaaaacgca attctcgggc tctacgaagg gtttgaagat ttcaaaaaag
      481 agcatccatc ttttggcttt gtgttgtcgc cgagttttaa aaaagaagag gggttctacg
      541 attcagacag cttcgccacc aaagaagaag attttatgaa ccaggtagac actctgcttt
      601 cgcttttaga caagtaccct gaattgcaaa cttgcgtaac cgacattgat accgtgggca
      661 acgatacgga aatgtaccga aaagcccact ttttccccat gcagcggggc atgcgcaaac
      721 tgcaacagta tggttttcat gtacggagcc accacggaga aacatggcac acgttaaacc
      781 acggcattca ggctgtggac aacgccatga atatctggca tatcgacagc ttggagcacg
      841 gcttgagcct tggcattaac cccaacttct atctgcatac ggttttcgaa aacgtgcggg
      901 aacaaaacat ggaaggcata aagctcgatc ctgcaagccc cgaaggcaag gaaatcgtag
      961 ctatggaatg ggggaaatac ggggacattt tcaaaaaact gcttgctggc gaactactca
     1021 ccgatgccga tgtaacgcac tttgcaagcg taaagtttgc cactgccatt gaagtggaac
     1081 attaccagca cgacgtatta aaccgcatga tcgaccagga aatcgggcta acgtcgcttc
     1141 cctcttcgaa ccgcaagctt tcgggcacca ttaccgactt tcgcaaccac ccgttttcct
     1201 ggtgggagaa aaaaggcgtt acccaggcta ttggaaccga caactatgtt accctaaaca
     1261 ccgattactt gcgggaaatg atgattttgc tcttgtctga cccaaaagga ttaaaaataa
     1321 ccaagctgct tatggtggct actggagaaa atcgccgccc gtacttgagc aaaacccttt
     1381 gggacatgcg aaagcagctc aaaaaagaat ttgcttagca tgatatacgt ttttgacttt
     1441 ggctctcaat attcccagct tattgccagg cgcatccggg ctttgggttg cgaggcaagg
     1501 cttgtgtccc accaatatcc tttgcaaaaa ctcaccgatg ctaaaggtat tattctttct
     1561 ggcgggccag attcggtgta cgaaaaggag gcagctagtg tttcaaaaaa aatctttgat
     1621 ctcggcgtgc cagtgctcgg catttgctac ggcatgcagc taacatgcca tgtattgggc
     1681 ggcaaggtgc agcgctctgc caagcacgaa tacggcccgg caaagttgga aatcgtgcga
     1741 aagagcgcct tatttgaaaa agtgccaaag tcttctgctg tgtggatgtc gcacggcgac
     1801 caggtggcaa ctcttcccaa aggttttgta caaacgggag ctagctccaa cacgccaaac
     1861 gccgccatgg aacagacagc aaaagatatt tacggcattc agtttcaccc cgaagtagaa
     1921 catacagagt acggctcgca aattctcaaa aactttgtgt tgaacatttg caaatgcaaa
     1981 cccgcaaaca agctcgccga gtttatcgag caaaaagtgg cggaaattca aaaaaccgta
     2041 ggcaaggaac acgtaatttg cgcaacttct ggcggagtag attcttcggt ggtggcagcc
     2101 ctcgtgggga aagccattgg aaaccaactt acctgcattt ttgtaaattc tggcacgctt
     2161 cggaaagaag aagacaaaga cgcgctcaaa ctcttacaag acttaggcct taacgtacaa
     2221 tacgtaaacg cagaaaaaca gtttttgcgt gccttgaaag gcgtaacata cggagaagca
     2281 aagcgcaaaa ttattggcaa agagttcatt gccgtgtttg agaaatcggc aaaacagata
     2341 cgcccaactc ctgcgttttt ggcgcagggg actattcatt cagacgtagt ggagtctgca
     2401 aaaactgcta ccggcaaaaa aacgcaaaca ataaaatcgc accacaatgt gggcggctta
     2461 cccaaaaagc tcaacttaaa gcttttggag cccttgcgcg acttgtttaa agacgaggtg
     2521 cgagaggtag gacaaaaact cggacttcca gaacatgccg tgtggcgcca gccttttcca
     2581 ggccctggtt tggctgttcg cgtggttggc gaagttacca agcaaaaact cgatatcgca
     2641 cgcgaagcag acgctattgt aacagaagaa atcgaggcgg caggccttgc tcgcagtttt
     2701 taccaatact ttgccatatt gctcaacgac aaaaccgttg gcgtcactgg cgaccagcga
     2761 aaatacgcat accctgtagt aataaaagca gtggtaagtt cggatattat gacttcagat
     2821 tgggcccgcc tgccttacga cttgctggct cgcatttcca cgcgcattac ttcggaggta
     2881 ccccaaatcg cccgcgtgct ctacgacata acccccaaac cccccggcac caccgagtgg
     2941 gagtaagtgt tcaaaatgtt caacatcgta aaaatgttca acatcgtaag ttgaacattc
     3001 ggtactctgt cgcttgcctg aaaatgcgct ccgtgctact tttcccttag taagggaatt
     3061 tttattcccg caaaaaacgc accaaatttt gtattccagg aggaaacaat ggttgcgata
     3121 gctgttatcg gaggccagtg gggcgacgaa ggaaagggca aggtcattga ttttctctgc
     3181 gagaatgcca gtgtcgtggc gcgcggcacc ggcgggaaca acgcagggca taccgtttgg
     3241 gtaggcaagg aaaaacgggt ttcccacctc gtcccatctg cagggttttg gcctggcaag
     3301 agagttattt tgggcaacgg gatggttatc aaccccgatg ttcttatcga agagcttagc
     3361 tggcttccgt atttgaatgg aaacgtgccc gaaaacgaaa gaacgcaaat ctttgtgagc
     3421 ccccaagcac atgtgattat gccttatcac ataacgcttg atgcgctcca agaggcgaaa
     3481 cgaggtgcag actctatagg gactaccggt caaggtatag ggcctgcata cgaagataaa
     3541 atgaggcgcg aaggagttcg tatcggcgac ctgcttaact tggaagaact attgccccgg
     3601 gtagcctctc ttgtgcaagc aaagaacgaa cttatcgaaa aggtatacga gggagagcca
     3661 ctcgacgtag atgaagtgca aagtcgcgtg gtttcatggg caaggcgtct tcttccctcc
     3721 attcgcgtgg aatatcacat atcttccctt ctgcaagaag cagttcaaac aggaaagctg
     3781 ctcttggagg gagctcaagg aacactacta gatattgatc atggaggcta tccacacgta
     3841 acctcatcga attctagcat tggaggtatg cttgtgggga ttgggctcac tcccaaaagc
     3901 ctaaaccaca tccttggtgt gttcaaggcg tatgcaactc gcgtgggctc tggagtgttt
     3961 cccacagaga ttctggacga acgtgccgac aaaatccgag atgccggaga agcaccagag
     4021 tatggaagta ctacgaaccg tccgcgaaga atcggatggt ttgatatggc ggcagcccgc
     4081 tacagtattg agctcaatgg tatagattcg atagcgctca cacgtttgga caccctcgac
     4141 ggcttcgaga aaatccgtgt atgcatgcga tatacatacg agaatgcgca tgtctcggag
     4201 tatagataca ttccagacga taaaacgctc accgagctgc gaccgaacta tggcgatctc
     4261 actggatggg acggcgtagc cggagtaaca aagtggaata aacttccaaa aacagcgcag
     4321 agctatgtgc gttttctgga agataacctc ggagttcccg taagtataat ctccacgggg
     4381 cccgagcgtt cctccacgat tgtacgacat caaattttct aaccaaaaac ccgccggttg
     4441 gataaataaa acaaccagcg ggatttcttt ttgcaaaaat tattgcgcgc taaagccgat
     4501 ggggggctcg ggagattcgg cttgctggat tttgccaaat ttggtttcgt atttttccac
     4561 gttgtcttgc aaggcagcaa gcattcgctt taagtggcct gggcttaata ccacgcggga
     4621 agccaacgta ccctgcgggg gaataaccat aaaaaagtcg agcacaaact cttcttgcgt
     4681 gtgcgtaatt tgcatgaggt ttgagtactt gccttttaag tcctcgtctt gcgctttaat
     4741 ttgaatttgc ctttgctgtg gctgttgctg gttgtccata aatgtaatta tatttaagtt
     4801 tatcttagtt tctgtatatt acgtttattt attttgagca agggaaatgc taggattaag
     4861 ttaaaataaa ttcgggggga tttgaaatgg cttggaagat tttgctcctt tgtgtcctta
     4921 ctctgctttg gataacttct gctgcttgcg tgcctgaagc acctaaaatt gtttccgaag
     4981 agtacgtaga agctaaagtt ttaggatctt cgtttgctcc attcggtggt tactatgtat
     5041 tgatcgctca taatggagtc gaaagttata ttgatattgc tggcaacgag agaaaatctt
     5101 tggaagctgc aggtaaggta aaggcatgcc tcgtaggtct tacaacaacc ttcaaggata
     5161 ctctccaata tgagttacga ccgaaatgca gtgactctat caaaggcggc cttatgtcgc
     5221 cctttctttt tggttaaaaa agctgttgcg aattcgtaag ttccgaagaa gagagccgta
     5281 taaaagaggt cgcccaaaag agtaaatttt aaaaagggca aggccatggt gtaagaaagc
     5341 ataaggccgc taaaggtgga cgcgtacatg ccagaaaata gccatacggc aaagtttgtg
     5401 gtgagataaa acaacagaga acccgaaacc gcagcaagcc ccagtgtttg aagcttcgct
     5461 tcaaacacaa acattcttgc aagaaggccg attaaggcga tggcaaaaaa gctggcgtat
     5521 accacggcca tggtttttgg ctcgtaaaag cccacaaata ggtctgaaac cagcattgcc
     5581 cccaaagggg taaaaaggta ccacttggaa acttgggagg cgtacacgcc cgcaaacaag
     5641 gccagagcag ctataggcgt aaaattcggc gcatgcggca acaacctaac tacaatgctg
     5701 aaaataacaa tacccgccag aaacacaaaa aagaattttt tgttcatatc taaaactcta
     5761 ctccctttaa tgccagttcg ccttggttat acggatgctt taactcgcgc atttcggtgg
     5821 ccaagtctgc ccgatcgata aattcttgcg gggcgtctct cccgctcaag ataacgaact
     5881 cgtgcatggg aagatctttg agtatagcta acacatcttt tactgccaag agcttcaggc
     5941 ttgtggcaac atttacctcg tctagaacaa ttaagtccca caggtgggac tcgattgcct
     6001 ttttggcgtg ctctagcgtt tcttccgccg cctttttatg ttcttcgaat ggaagggtat
     6061 cgcccaaaat tcccacaaac cccttgcctc ctttgtgaat ttcccacttt ccttcaaact
     6121 gcagatgttt ttgcaaaaac ttgtcttccc cggaaatcca cggccccttg ataaactgaa
     6181 acattatggc tttcctatct tgtcctacta tgcgcatcat ttgtcctagg gaagccgtag
     6241 tttttccttt cccatttccg gtaaatacca cgatcatgtt acatacatcg gctaaacccg
     6301 cacgccttgc agctaatgca cccttccgac attaccaatt gagctccgca gtcagggcac
     6361 cctggcataa agccgaaatc tgccatagac tgggtctcct ttgcaaacgc aaatacttcc
     6421 tgcttttctg gccgctctaa aatgtcttca acctgtttta ctcctgtaac atgttcttcg
     6481 agcactcttc caatggcatc tggcaacgaa agcacggtgc ccttttcggt aaatatgggc
     6541 atgggacctc gaattccctt aaggtcgctt atcacctctt ctacccgaat gccggcgcgc
     6601 aaggcaagcg acgttaacct gcaaatagct tccgactgct cctggaaaaa ccctccgctt
     6661 ttgccaatgg tggcaaaaat ttcaaaggga tactcgttct cgtcgttgtt aatggtaatg
     6721 tacaaattcc catagccggt tttcatgcgg taggtcttgc ctgccattat ttcagggcgc
     6781 ttgcggggca ccacctcccc ccgttggcct tgttgtgggc tcgcagaagg cctttctgaa
     6841 ggcttttcgg tggtagaaac catgttgtcg ccagaaccca cgttgagaat ctgaatggtg
     6901 cggctcccgt cgcgatacac cgtggcagat ttgcaaccca gcttccaggc agctacaaaa
     6961 ctctgggcta cctcttctct ggtagcggaa ttcgggaagt taatggtttt ggaaatagag
     7021 ttatccacat gcctttgaaa cgcagcctgc atttccatat gggcctggcc cgaaatatcc
     7081 attgccacca taaaggtatc gcgaatttct tcgggcagcc agtggacgtc ttgaatactt
     7141 ccttttgcca taacctggtc cataatttct ttttgctgtt cttctgaaag ctgcaggctg
     7201 tcgagcgcct gctggaaata tttgttgaag taccggtact gctggccgtc tttgtcttgt
     7261 tttacataag ccaaagcaaa gttgggctct accccagagg aggtatcgaa catcatggaa
     7321 atggaaccgg tgggggctac cgtggttaaa gctgcgttgc ggcgctttat gcctgcagct
     7381 gggagaatgc ttttatccaa attgggaaat tcgccttttt ctcgagccaa ctcttctgaa
     7441 gccaaatgag attctttgtt gatgaaaccc attacttgtt ctgcggcttg catgccttct
     7501 ggcgagtcgt agcgaatgcc caactggtag agcatgtcgc caaaacccat aatccccagg
     7561 ccaatcctgc ggtttttctt ggcaagttcg gttacctggg gcacgggaaa atcgaacttg
     7621 tcgattacgt tgtccattaa gcgaaccgcc gttcgggtaa caaaacccag gcgctcccag
     7681 ttgattttgc cgtctgccac gaattgggca aggttaatcg accccaaatt gcaattgtcg
     7741 tacgcgtgca aaaactgctc tccgcatgga ttggaagcag caatgggccc caggccgggc
     7801 aaagggttgg tacggttaat ggtgtcgata aaagcaatac cgggttctcc gttaagccat
     7861 gcgtgttcta ccagcttgtt gaaaagctct cctacggtaa tatcaatttc ctctgaacca
     7921 tacactgcgc cgttcgggta acggagcacc tggtggggct tcattttctg gcctcgaaac
     7981 tggcagtacc acagctgcct ggggttttct tcgagctgtt gcataaactc gtcggttacg
     8041 gtaactgaaa tattgaaatt ccggatatca ccctccacct ttttgcactc aatgaaatcc
     8101 aacacatcgg ggtggtctac ccgtatcata gccatattgg ccccgtgcct gccctgttgc
     8161 tttatggtgc caaaggcctt gtcgtatacc gacaaaaagg ttacgggccc cgatgctctg
     8221 ccgcgcgatt ttttggtggg ggagtttgcc ggcctcattt cagacaagtc aaaaccaagg
     8281 ccgcaccctg cctgctggag aagcgccgct tccttgagtg tctggaaaat gctttccatg
     8341 gaatcctcaa tgcgcagcac aatgcagttt gcaataagcg gggtttcccc accagcattc
     8401 gtgagagtac ggccggctgg cgtgtactct tttgaagcca taacggcaaa aaagtctctt
     8461 gccgtttgct ctatgagttc ctctggtttg ccgtagtctt tttctacctg tgccaaagcg
     8521 tttgccacgc gctcaaacat gtcggcagga gtttcctggc tcccctcgtc gttaagcatt
     8581 aaataccgct tttgcatcat ttttaaagcg ttttctgaaa aggtgctgtt ggtttggtcc
     8641 ttttgttctt gagaaactcg catggttgag agttggggta taaattaact tttatgcggc
     8701 gacggttgtt gaagcttttg aatttccttc tcgaaagcct tggggcttcg gaaatttcgg
     8761 tacaccgagg caaaacgcaa gtaagcgacc ttatccaagt cttttaagtg agccaatacg
     8821 gcttgcccaa tggtgcccga atcaacgaca tctttgtcga gattaaaaat gtcgttttca
     8881 atgcttgaaa cgagcttttg aaggtgttcc tcgccaaaag ggcgcttttc tagggccttt
     8941 ctgatcccgg cttccagctt atggcgggaa aagggttctt tagccccatt tcgctttaaa
     9001 acctccactc caagcaattc caaacgttcg tacgtggtaa atctccgccc gcaggaagca
     9061 cacaatcggc gccttttgac tgcccgtccg tggtcggcat ccctggagtt agtaaccttg
     9121 gtgtctatcc gacaaatagg gcattgcata cctcaaaacg tcaatatata gtatagaaaa
     9181 taggtaaagt agtacagata gcagcgccga tgcactacca attttatcac tcgggcaatg
     9241 cgtgtcaatc tgcgtactgt ggataagtag agctgtccta taccaaacct tgactactgc
     9301 cgatttgccc tccctacggc aagggcttga cttttttagg gttatggtat acaataagca
     9361 taatcccaaa caggggattt tttattaaag ctatatgaac caaatacaac aatctgtcgc
     9421 agccgccatt ttagtgccca ttttaagcat aatgccggct ctcgacgctc ataccgcaga
     9481 cgatgaagac ccttcgccta ttggagagag attggtaatg tcccaaacca atactctcct
     9541 ccccatagct tctccgatgg gtcctgaacg ggtagcacag actatgcggc tctacgtaac
     9601 cggatattct tctactcccg aacagaccga tgatactccc tttatcacgg cttccgggaa
     9661 gcacgtacga gacgggatcg tggcaacaaa ccttcttcct ctgggaacga agattaaaat
     9721 tcccgagctc tacggcaaca aagtatttgt ggtggaagac cgcatgaata ccagattgca
     9781 aaaccatgta gatatctggt ttgccgagta ttgggacgcc aaaaactttg gcatcaagcg
     9841 cgc
//
LOCUS       DASRUP010000056         8689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1504856, whole genome shotgun sequence.
ACCESSION   DASRUP010000056 DASRUP010000000
VERSION     DASRUP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504856"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFE94_02215"
     CDS             <1..516
                     /locus_tag="VFE94_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZX49945.1"
                     /translation="SVAPESVKGKELLSRMEHPISLDQASAADYDAIFVAGGHGALFD
                     LNKNKNLHQLLLRFDEAKKPLGLLCHASSALAFMEKDGKPFLQGRRITGFPTAWEHGV
                     LQTNSVHKDFLPLPLWTGKEIEKAATGRTWLDRIGEVVNPWYTIQDKNLITGVGPKTG
                     AKLARLVTQYE"
     gene            509..868
                     /locus_tag="VFE94_02220"
     CDS             509..868
                     /locus_tag="VFE94_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49946.1"
                     /translation="MNSLTKASTSVYVFGIYMIGQGLTLLLAPNMLLQFMQLPETNEV
                     WTRLLGVTLMVLGYYYIRAARVNFLEFFGWTVHMRLAQFGIVGALVLLNIAHPLVLGF
                     AAVEAASGLWTFWALRK"
     gene            870..1616
                     /locus_tag="VFE94_02225"
     CDS             870..1616
                     /locus_tag="VFE94_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZX49947.1"
                     /translation="MDYFGLLAVTFFLSFGVHIWSALVGGGGAIIIPALLFLGFPPQV
                     AVATNRVGALSNIFALFQFHRHGEVKWKVGLWLALFAGAGSAIGSFLLLQVEADVLER
                     GIAVIILLSLPMFLLRPKMGLKERKVRLTKQRQVGGGAIMFFLGILGGFFSATGVWFR
                     YVYLFYYGMTFLQTAATSKIAGMAMLVLSLAVLIPAGIIDWPVAGAMFAGGSLGSWLS
                     AKYSKKLGNEKIRYLFLAVMFAMALKILLF"
     gene            1674..2282
                     /locus_tag="VFE94_02230"
     CDS             1674..2282
                     /locus_tag="VFE94_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49948.1"
                     /translation="MKAWVVAGISFFVIGLVLAVLFVNILWGDSPGNETGTREVTASV
                     NADPAQINPGGSATLSWSSTNATVCSGVNFPTEGKESGSVQVAPSEVTQYDLICSNDT
                     DVVVESITVAFAQGVPTVSVSSSASLVAPGQPVTLTWSSGNADQCSSAEFSAQGAVSG
                     SIVVAPTGTTTYTVDCIQVPLFATSHDSVLVTSGSVTVQVSS"
     gene            2289..4928
                     /locus_tag="VFE94_02235"
     CDS             2289..4928
                     /locus_tag="VFE94_02235"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HZX49949.1"
                     /translation="MKKLILLFLVLVVAVAAAYAVFQTAEQVQAAAISSAGSGNWSNA
                     STWNGGRVPGAGDSVTISGGHTVTYDVTDSQVSGVTIIGNGRLVFASGKSVVLRTDRN
                     IEVLGRLEMKPSSASIQHTIRFVGIDETRMVGGGMNTLASDVGLWVMGAGSLDIAGSA
                     KTAWTRLQGGANAGSNTIQLQQAPNGWRVGDEIVVVPTEPTSAGDASWNGFELRTIAS
                     ISGSTITLNSGLSRNHPVATDPKTNRAFTAEVLNLSRNVRIEGTGNGSANPNQNGRAH
                     ILIRSTAPQFVKYAELKLLGPRRFTGESNFTAGVLGRYGLHFHHSENGSIGSLVEGTV
                     VRDTGGHAFAPHASDGIIFRNTISYNTFDDAYWWDPPPQLPGRPGVNSTNPINDSDDI
                     LYDNAVAALTKFDPAFRGSRLSGFWLGPGLRNAVRNSVAVGIQGGNVGVSDSSGFIWP
                     EAGSGRTECCSGVWEFNQGNISHNNKNHGIFTWQNTSHLHIVSNVALYNNANSAVDHG
                     AYVNSYRYVDVIAFGNGKYGLQVHSAARNVQLRPDGYSQSFERFHTTKPIIFPNHNSL
                     TGIPTLLKDCTFTGVVVNDARTDQNFPTEADFCNCAKPNGQSIEPADFTFQNVEPGLD
                     IRVERPNGTAYHITNGGVVTNIAPFCSSFSLPPVGPTQPTQQPPTQQPQTQQPSTQQP
                     STQQPSTQQPGQSGGQSSQPAAAAPAVCGDGKIEGSEKCEPGNLAGQTCQTGGYTQGT
                     LACHPAGSAQQCQYDVSGCSGIAGLVPCGQLGNPCNLCHIFKLVNNVLVFLLIPDGSV
                     NNFIPLVLVIAGLLIALGGFFMMTSAGNLANLARGRTIIFAVVVGLLIMYGAWLFIGF
                     LLQAVGFVSFNGGLPWNEISCGN"
     gene            complement(4941..5615)
                     /locus_tag="VFE94_02240"
     CDS             complement(4941..5615)
                     /locus_tag="VFE94_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZX49950.1"
                     /translation="MENQLIIVYHKEMGLLNLFFPKQCISCHREGNSLCEDCIALVSL
                     NQTVSPLPANSALSGLFCATSFKDPNVKYFIHSLKYPPFLRDLAKPLAFLIVSHFAIL
                     NNTLFTPDMIIPVPLHTRRLHWRGFNHAEEIAKHLSFAWEVPLESKALQRIKYTAPQV
                     ELGKEKRHANVKGMFATPQPSFVQGKTIFLIDDVYTTGATMQECASVLKKAGAAKVFG
                     AVVARD"
     gene            5599..6654
                     /gene="pilM"
                     /locus_tag="VFE94_02245"
     CDS             5599..6654
                     /gene="pilM"
                     /locus_tag="VFE94_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HZX49951.1"
                     /translation="MSWFSIDIIPKDFLGIDVGSSALRIVEIGGWADRRSVKNYGEIR
                     IRTMYEEPFRTFEKNSLLLSTADIAKAIRGIMEELGMKEKKAVFSISDFSSFFTILEL
                     PPMKEQELGDAIRFEARRHVPLPLSEVVLDWQIFERTTKQEPHRILLVAVPREVVNYY
                     EEIARFAKLKLIALEAEVFGNIRSYLKEEKFPVMLLDVGSHTTTVSVVHKGYLRVSHT
                     IDTGGNSFTERIARGLSIDYKEAQDRKVTEGMDIVAGNVRILLPVVDVILGEMRKALD
                     IFERQKVGEVHKIVLSGGSARLPGLKEYIEKEFRIRTEIIDPFRHLLYPPLLEEEIKK
                     MGPSYAVAVGMALRGFE"
     gene            6659..7198
                     /locus_tag="VFE94_02250"
     CDS             6659..7198
                     /locus_tag="VFE94_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49952.1"
                     /translation="MAIELIPRREEKPVFGRPFFLIISTVVFVGVAAGVLVLEQLASA
                     KKDELNSLQRTFVQDTRPQEEELARQLQEYRAKIENLRLALDQRQDVLWLFEFLEDTL
                     HPDVFLTEFENMRENSFVFQGEAVNAFVLEQQRLVWEGQEGMQSVELKEIELLSSGRV
                     EFQVEFVPAADFFGNISFL"
     gene            7195..7737
                     /gene="pilO"
                     /locus_tag="VFE94_02255"
     CDS             7195..7737
                     /gene="pilO"
                     /locus_tag="VFE94_02255"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HZX49953.1"
                     /translation="MRKVIEQFLPIFILVVAVLGGVFFLWPAYEDFSGINTQVNIQQE
                     RLERGQRVLTQLVALQRQIAERQEEFEKLEYSIPEDMQLPAVYDLIQQLAASSGLVVE
                     TITTQGTEVAGESLDIVHVEVQLVGSYAGLKNFLDAAKRSARIVNVGTLHIIAAAQPG
                     AQSALAIEAEIAAYANTDNP"
     gene            7741..8016
                     /locus_tag="VFE94_02260"
     CDS             7741..8016
                     /locus_tag="VFE94_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49954.1"
                     /translation="MAFTFSPEQQNQHFLFGVLGVLVGVGAIFAWFAFFRDPPISLFQ
                     EPPPPPQEVRVDFEVFTHPAFLELGEARPPIPVPALQEVGKQDPFLP"
     gene            8022..>8689
                     /locus_tag="VFE94_02265"
     CDS             8022..>8689
                     /locus_tag="VFE94_02265"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49955.1"
                     /translation="MDILELLLAKHILKKEDVQLVEKELQSSGRKAEELLVQKRKVDE
                     DLLFQVKSEALGMPLSQVETSEIPLKVLELIPEDSAKYYKMVPLVSSRGRLEVGMVYP
                     EDAKAQEALQFLARQGNFSYTVSLITLSTFEQLMKQLRSLKSEMTKALQELEQEIGKG
                     EEGKAAAPETGQVIEDAPVTKMVAVILRNAVEGGASDIHIEPGREALRVRFRFLGDLH
                     ASLML"
BASE COUNT         2052 a         1979 c         2400 g         2258 t
ORIGIN      
        1 agcgtggcgc cagaatccgt aaaagggaaa gagctgcttt ctcgcatgga gcatcccatt
       61 tcgttagacc aggcaagcgc ggcagattac gacgccattt ttgtggcagg cgggcatggg
      121 gcgctgtttg acttaaacaa aaacaaaaat ttacatcaat tattgctgcg gtttgacgaa
      181 gcaaaaaaac cattggggct tttgtgccac gcttcctcgg cgcttgcttt tatggaaaaa
      241 gacggcaaac cgtttttgca aggcagaaga attactggat ttcccacggc gtgggaacat
      301 ggcgtgttac aaaccaacag cgtgcacaaa gattttttgc ctttgccttt gtggaccgga
      361 aaagaaattg aaaaagccgc aactggccgc acgtggctgg accgtatcgg ggaagtcgtg
      421 aacccatggt acaccataca agataaaaac ctaataaccg gcgtgggacc gaaaaccgga
      481 gcaaagctcg cgcgcctggt aacgcaatat gaatagttta acaaaagcca gtacaagcgt
      541 gtacgtattt ggcatataca tgataggcca gggccttacg cttttgctgg ctccgaatat
      601 gttgctgcag tttatgcagc tgcccgaaac caacgaagtg tggactcggc ttttgggagt
      661 aacgcttatg gtgcttgggt attattatat ccgtgccgcc agggtaaatt ttttggaatt
      721 tttcggttgg accgtgcaca tgcgtttggc ccagtttggc attgttggcg cgctggtgct
      781 gttgaacatt gcccatccct tggtgttagg ctttgccgcc gtggaagccg cttccggcct
      841 gtggactttt tgggcgctgc gcaagtaaca tggattactt tgggttgctg gcagttacat
      901 tttttttgtc gtttggcgtg catatatgga gcgcgctggt ggggggcggg ggcgccatta
      961 ttattccggc tttgctgttt ttgggatttc cgccgcaagt ggctgtcgcg accaaccgcg
     1021 tgggcgcgtt gtccaacata ttcgcgctgt tccagttcca caggcacggg gaggtaaagt
     1081 ggaaggtggg tttgtggctg gccttgtttg ccggagccgg aagcgcaatt ggcagttttt
     1141 tgttattgca ggttgaagct gacgtgcttg aacgcggcat tgccgtaatt attttgcttt
     1201 ccttgcctat gtttttgctg cggccaaaaa tggggttaaa ggaaagaaag gtacggctta
     1261 ccaagcagcg ccaggttggc gggggcgcca ttatgttttt tctgggaatt ttgggcgggt
     1321 ttttttcggc caccggagtg tggtttaggt acgtgtattt gttttactat ggcatgacgt
     1381 ttttgcagac cgcggccacc agcaagatag cgggcatggc catgctggtc ttgtctttgg
     1441 ccgtgcttat accggccggc attattgact ggcctgtggc aggggcaatg tttgccggag
     1501 gcagcctggg ttcgtggcta tctgccaaat actccaaaaa actgggaaac gaaaaaatcc
     1561 gttatttgtt tttggcggta atgttcgcaa tggcgttaaa aattttgctt ttctaagcgc
     1621 gttttcggtt gtgcacattg accgaccctt tgcgtttgat agaataaaag aacatgaaag
     1681 cgtgggttgt tgctggcata tcattttttg tcataggcct ggtactggct gttcttttcg
     1741 ttaacattct ttggggcgat tctcctggca acgaaactgg caccagagaa gttactgctt
     1801 ctgttaacgc agatcccgcg caaataaatc ccggaggaag cgccactttg agctggtctt
     1861 ccacgaacgc cacagtctgc agcggcgtaa actttcctac cgaagggaag gagtcgggtt
     1921 ccgtgcaggt tgcgccaagc gaagttaccc agtacgactt gatatgttct aacgatacgg
     1981 atgttgtagt tgagtcaata acggtagcct ttgcgcaagg cgtcccaacg gtttctgtgt
     2041 cctcgagcgc gtctttggtg gcgccaggcc aaccggttac cttaacctgg tcttctggga
     2101 atgccgacca gtgttcgagc gccgaatttt ccgcgcaagg agcagtttcg ggcagtattg
     2161 ttgtcgcgcc aaccggcacc acaacgtata cggtagattg cattcaggtt ccgttgtttg
     2221 ccacttcgca tgattctgtg cttgtaactt caggctccgt taccgtacaa gtgagttcat
     2281 aaacttccat gaaaaaactc attcttttat ttctcgtcct ggttgtcgcg gttgccgcgg
     2341 catacgctgt tttccaaacc gcagaacagg tgcaggctgc tgctatttct tcagcaggca
     2401 gcggaaactg gtcgaatgct tccacatgga acggcggaag ggtgccggga gcgggagatt
     2461 cggttaccat ttccggcggc cacacggtaa cttacgacgt taccgattcg caagtgtcgg
     2521 gcgttaccat tattggcaac ggcaggctgg tttttgcttc agggaagagc gtagtgctgc
     2581 gtacggatag aaatattgaa gtactgggta ggctggaaat gaaaccttcg tccgcaagca
     2641 tccagcacac catacggttt gtgggtatcg acgagacgcg aatggtagga ggaggaatga
     2701 atacgcttgc ctctgatgtt ggcttatggg ttatgggagc aggttctttg gacattgcgg
     2761 gatctgccaa aaccgcgtgg accaggctgc aggggggagc taacgcggga tcaaacacta
     2821 ttcagcttca gcaggctccc aatggctggc gcgtgggcga cgaaattgtc gtggtaccca
     2881 cggaacctac aagtgcggga gacgcttcgt ggaacgggtt tgagcttcgc actatcgcca
     2941 gtatttccgg aagtacgata acgctgaact ccggtttgtc gcgaaaccat cccgtagcaa
     3001 ccgacccaaa aaccaacaga gcgtttacgg cggaagtgct taacctttct cgcaacgtgc
     3061 gcattgaggg tacggggaat gggtctgcca atcctaacca aaacggaagg gcgcatattt
     3121 taatccgttc caccgcgccc cagtttgtaa aatacgcgga actcaaactg ctgggccccc
     3181 ggcgttttac cggcgagagc aactttaccg cgggcgtgct gggccgttat ggtctgcact
     3241 tccaccattc agagaacggt tctatcggtt ctcttgtgga agggacggtt gtgcgggata
     3301 cgggcgggca cgcgtttgcg cctcacgcgt ccgacggcat tattttcagg aataccattt
     3361 cctacaacac gtttgacgac gcctactggt gggatcctcc gcctcaatta cccggtcgcc
     3421 caggcgttaa ctcaaccaac ccgataaatg acagcgatga tattttgtac gacaacgcgg
     3481 tagctgctct tactaaattt gaccctgcgt ttcgcggaag tcgactttcc gggttttggt
     3541 tgggacccgg gcttcgcaac gccgtccgta attctgtagc agtcggaata cagggaggca
     3601 acgtaggagt atcggattca agcggtttca tatggcctga agcgggtagc ggccgtacag
     3661 aatgctgttc tggcgtttgg gaatttaacc agggcaatat tagccacaac aacaaaaacc
     3721 acggtatttt tacctggcag aatacttccc atcttcatat tgtcagtaat gtggccttgt
     3781 ataacaacgc aaacagtgct gtagaccatg gggcatacgt aaacagctac cgatatgttg
     3841 acgttatagc ttttggcaac ggcaagtacg ggctgcaggt acactctgcc gccagaaatg
     3901 tccagttgcg cccggacggc tacagccagt cgtttgagag gttccatact accaagccca
     3961 taatattccc aaaccacaat tcgcttacag ggattccaac tttactgaaa gattgcacgt
     4021 ttaccggagt tgtcgtgaat gacgcaagga cagatcaaaa ttttcctacc gaagctgatt
     4081 tttgcaactg cgccaaaccc aacggccaaa gcatagagcc ggcggacttt acgtttcaga
     4141 atgttgagcc tggcctggat attcgtgtgg agcggcccaa tgggactgcc tatcatatta
     4201 ccaacggtgg tgtggtaacc aatattgcgc cgttttgctc gtcattttcc cttccgcctg
     4261 ttggtcctac gcaaccaacg cagcaacctc cgacacagca gccgcaaacg cagcaaccct
     4321 cgacacagca gccttctaca caacaaccat ctactcaaca accaggccaa tctggagggc
     4381 aatcttccca acctgccgcc gctgctcccg cggtttgcgg agacggcaaa atagaaggca
     4441 gcgaaaaatg cgagcccggt aaccttgccg ggcagacctg ccaaacgggc ggatataccc
     4501 agggcacgct tgcttgccat cccgccggca gcgcgcagca atgccagtac gacgtttctg
     4561 gttgcagcgg cattgcggga cttgttccgt gcggccaatt ggggaacccg tgcaacctct
     4621 gccacatctt caagttggta aacaacgtac ttgttttcct attaattccc gatggcagtg
     4681 tgaataactt tatacccctt gtgcttgtaa tcgccgggct tctgattgcc ttaggaggtt
     4741 tctttatgat gacatccgct ggaaatttgg cgaaccttgc gcggggaagg acgattatct
     4801 ttgcggttgt agttggtctc ctcattatgt atggcgcgtg gctctttatc ggttttctgc
     4861 tgcaggcggt aggttttgtt tcgttcaacg gaggacttcc ttggaacgag ataagctgcg
     4921 gcaattaacg cgcaccaatg ttagtctcgc gctaccacgg cgccgaatac tttggcagcg
     4981 ccggcttttt taagaacgct cgcgcattct tgcatagtgg ctccggtggt gtacacgtcg
     5041 tctatgagga aaatggtttt gccttgcacg aacgatggct ggggcgtggc aaacattccc
     5101 tttacgtttg cgtggcgttt ttctttgccg agctccactt gaggggcggt gtattttatt
     5161 ctttggagcg cttttgattc caatggaact tcccaggcga aagaaaggtg ctttgcgatt
     5221 tcttctgcat ggttaaatcc gcgccagtga agcctccttg tatgcagggg aacggggatt
     5281 atcatgtctg gtgtgaataa tgtgttgttg agtattgcaa aatgcgatac aataagaaaa
     5341 gcgagcggtt ttgcaaggtc gcgcaggaaa ggcgggtact tgagggaatg aataaagtac
     5401 ttcacgttgg ggtctttgaa agaagttgcg caaaacaaac cagacagcgc ggagtttgcc
     5461 ggtaaaggag aaactgtctg gttcagagaa accaaagcaa tgcagtcttc gcatagagag
     5521 ttcccttctc ggtggcagga aatgcactgt ttggggaaaa aaagattcag cagtcccatt
     5581 tctttgtgat atactattat gagttggttt tccattgata ttatccccaa ggactttctc
     5641 ggcattgacg tggggagttc ggctttgcgc attgtggaga ttggaggatg ggcggatcgg
     5701 cgaagcgtta agaactacgg ggaaatacgg attcgtacca tgtacgaaga gccctttcgc
     5761 acttttgaaa aaaacagcct tttgctttcg actgccgata ttgcaaaagc aattcgtggt
     5821 attatggagg aattggggat gaaagaaaag aaagcagttt tttccatatc agatttttca
     5881 agctttttca cgatactcga actccccccg atgaaagaac aggagttggg cgacgcgatt
     5941 cgctttgaag cgcgaaggca tgtgcctctt ccgctttctg aagtggtttt ggactggcag
     6001 atattcgagc gcacgacaaa acaagagcct catcgaattt tgctggtggc ggttccccga
     6061 gaggtggtaa actattatga agagatagca aggtttgcga aactgaaact catagcgctg
     6121 gaggctgaag tatttgggaa cattcgttcc tacctgaaag aggagaagtt ccctgtgatg
     6181 ctgctggatg tcggttctca cacgacgacg gtaagcgttg tgcacaaggg gtatttgcgc
     6241 gtttcccata ccattgatac gggcgggaat agttttaccg agcgcattgc gagaggactc
     6301 tcgatagact acaaagaagc gcaggaccgc aaggtaaccg aaggaatgga tattgtggcg
     6361 ggaaacgtga gaattcttct gcccgtagtc gatgtgattc ttggagaaat gcgcaaagct
     6421 ctggacattt ttgagaggca aaaagtgggc gaagtgcata aaatcgtgct ttcgggaggt
     6481 tcggcaagac ttccggggtt aaaagagtat atcgaaaagg aatttcgcat aagaacggag
     6541 attatcgatc ctttccggca tttgttgtat cctcccctgc tcgaagaaga aattaaaaag
     6601 atggggccat cgtacgcggt tgccgtagga atggctctgc gaggttttga ataatattat
     6661 ggcgattgaa ctcatccctc gacgcgagga aaaaccagtt ttcggaaggc cttttttcct
     6721 tattatttca acagtggtgt tcgtaggagt ggctgcggga gttttggttt tggaacagct
     6781 tgcctctgcg aaaaaagacg aattgaactc ccttcaaaga accttcgtgc aggataccag
     6841 gccgcaggaa gaggaacttg cgagacagct tcaagaatac cgggcaaaga tcgaaaacct
     6901 gcgattagcg ctcgaccagc gccaggatgt tctttggctt tttgaatttt tggaagacac
     6961 gctgcatccc gatgtgtttc tcacggagtt tgaaaatatg cgagagaaca gtttcgtctt
     7021 tcagggggag gcggtgaatg ctttcgtact ggaacagcag cgtttggtat gggaaggtca
     7081 agaaggcatg caaagcgttg agcttaaaga gattgagctt ttgtcgagcg gaagggttga
     7141 gtttcaggta gagtttgttc ccgccgccga ttttttcggg aatatatcat ttctatgaga
     7201 aaggttatcg aacaatttct gcccattttt attctcgttg tcgcagtgct cggcggagta
     7261 tttttcctgt ggcccgcgta cgaagatttc tctggcataa acacgcaggt gaatatccag
     7321 caggaacgtt tagagcgcgg ccagcgtgtt cttacgcaac tggtggcgct ccagcggcag
     7381 attgccgagc gccaggaaga gtttgaaaaa ctcgagtact ccattccgga agatatgcaa
     7441 ctgcccgccg tgtacgattt gattcaacag cttgccgctt cttcggggct tgttgtagag
     7501 accatcacga cacaaggcac tgaagttgcg ggagagtcgc ttgacattgt ccatgtagag
     7561 gttcaactgg tcggttctta cgcgggattg aagaactttt tggatgcggc aaagcgatct
     7621 gcaagaatag tcaatgtcgg aactttgcac attatcgccg cggcgcagcc tggcgcgcaa
     7681 tccgcgttgg ctatcgaagc tgaaattgcg gcctacgcaa atacggataa cccataaatt
     7741 atggcattta ccttttcccc agaacaacaa aatcaacatt ttcttttcgg agtattggga
     7801 gtgctggtag gagtaggagc gatattcgcc tggtttgcgt ttttccgcga cccacctatt
     7861 tctttgtttc aagagcctcc ccctcctccc caggaagtgc gcgttgactt tgaggttttt
     7921 acacatcctg catttctgga actgggagaa gccagacccc ccattcctgt tcctgcttta
     7981 caagaggtgg gcaagcaaga tccttttctc ccgtaagctc tatggatatt ttggaacttc
     8041 ttttagcaaa gcatattctc aaaaaagagg atgttcagct ggtggaaaaa gagcttcaat
     8101 cctctggcag gaaagccgag gagctgttgg tgcagaaaag gaaagtagac gaagaccttc
     8161 tcttccaggt gaaaagcgag gctttgggta tgccgctttc gcaagtggaa acgtcggaga
     8221 ttcctttgaa agtactggag cttatccccg aagattcggc aaaatactac aaaatggtgc
     8281 ctctggtttc ttcccgcggc aggcttgaag ttggaatggt atatcctgaa gacgctaaag
     8341 cccaggaggc tttgcagttt ttggcgcggc agggaaattt ctcttacacg gtttcgctta
     8401 tcacgctttc cacgtttgaa cagttaatga agcagttgcg cagcttaaag tcggagatga
     8461 caaaggcttt acaggaactc gaacaggaaa ttggcaaggg agaggaaggg aaggccgcgg
     8521 ctccggaaac agggcaggtg attgaagatg cgccggttac gaaaatggtt gcggtaatac
     8581 tgcgtaatgc cgtggaaggc ggagcttccg atattcacat tgagcccggg cgtgaggctc
     8641 tgcgcgtgcg gttccgtttt ctgggagatt tgcatgcttc tttaatgct
//
LOCUS       DASRUP010000057         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_603008, whole genome shotgun sequence.
ACCESSION   DASRUP010000057 DASRUP010000000
VERSION     DASRUP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603008"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFE94_02270"
     CDS             complement(<1..277)
                     /locus_tag="VFE94_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49956.1"
                     /translation="MDITRLSATRFLVRYVKTYKIKSALIGGALLVLAGMGVQTLLQQ
                     AGGEPTLERTPRVSLLNVDEYRLENAVVESVGEVESLSQVELKSQVSE"
     gene            complement(329..1699)
                     /gene="purD"
                     /locus_tag="VFE94_02275"
     CDS             complement(329..1699)
                     /gene="purD"
                     /locus_tag="VFE94_02275"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZX49957.1"
                     /translation="MDSIDVLVVGEDARTNMLCLKLAESPLLRKLYCFRGNAGTAQVA
                     ENIVLRTDRKDTFEDQVVKLAAALGVGLVVVGPEDPLINGVADKLLTVGIPTFGPRAK
                     AAFKTEGSKVESDRLARRRGVPRPKAWVARSMADVERCLAENGNQPVVVKPDKPTGGK
                     GVTVPKTVEEVWAAAFACLEEGAFNNAGRVVLLEELLEGPEVSVFVFTDGRRVSRPFS
                     ARDRKLRFAEREKREHNPNTGGMRAIAPAPFWNLGLEEEIMETIMRPMVAELLWQDAM
                     YRGVLYAGIILTPEGPKVLEFNCRFGDPEAQAILPLLKSDLLEVMLACAQGNLHKMQV
                     EWDYERWCIAINLVDEAYPGQPQNGGRITGLEAEQEGTFVLHYGTTLLECSDGRKSYR
                     LNGSGRRISVGGIGGTREEAILRATYRVAGETPIEFPGLVWRQDVEIEEEGLPGELLE
                     RYSYVA"
     gene            2129..2887
                     /locus_tag="VFE94_02280"
     CDS             2129..2887
                     /locus_tag="VFE94_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX49958.1"
                     /translation="MAKTISACMQNPWVKVIVDSALTAEEGLQKAGQNPPHFVVMRPD
                     FTDASLARAIGELKSFSSTPILVLGYHGSAAEEIDALAEGADEFVSFPPRDLKVLEIR
                     IWNVLRTRAGMFLQPVEEMAALTSGELMLNPGACEISLNTKRMQLTPTEFRVLYLLMR
                     NRGNVLSHQFFGDSIWGDEVDSSGLVKKYIQKLRRKLGDDVEEPRWIDTVPRVGYRFV
                     GPVVRAIPPMPPLATFEDLTEDPPPDQRSLMAPV"
     gene            complement(2944..>3766)
                     /locus_tag="VFE94_02285"
     CDS             complement(2944..>3766)
                     /locus_tag="VFE94_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49959.1"
                     /translation="TVRAFCHPLVHVIGRAATQKYPDVSQAYTKGDHFCWSGYYHGVM
                     EGILYETAPEDLPGVINSICQNIPGRESYSFDYYNCVHGLGHGVMYITQSELFEALAL
                     CDNLQGGWEKESCYGGVFMENVITDFENHFTKYLKPEEPLYPCTAVPEKNKHACYLMQ
                     TSYVLKVNGYNFADAFVQCSTIEEQHKDTCYQSVGRDASGSTVSDVARTKAYCLLGTD
                     ERQQTNCVIGAVKDFISYFHSDVQAKQLCNAFSDYTQQVCLNTAASYYQSFQKDI"
BASE COUNT          919 a         1008 c          864 g          975 t
ORIGIN      
        1 gttctgacac ctggcttttg agctccacct gcgaaagcga ctccacctcg cctaccgact
       61 ctacaaccgc gttttccaat cggtattcgt ctacattcaa aaggcttacc cgtggcgtgc
      121 gctcgagggt gggctcgccg cctgcctgtt gcaacaatgt ttgcaccccc atacctgcca
      181 aaaccagcaa agctccccca attaaggcgg attttatttt ataggtcttt acgtatcgaa
      241 caagaaatcg agttgcggat aatcgagtaa tgtccataaa ccagccatag tatacaccaa
      301 aaaagcagga ggtcaaagac ctccgtgatt acgctacgta agagtacctt tccagaagtt
      361 ctccgggaag gccctcctct tctatttcga catcctggcg ccaaacaagg cccggaaact
      421 caatgggcgt ttctccggca accctatatg tggcacgaag aatagcctct tcccgcgtgc
      481 cccctattcc gccaacgcta atccttcgtc cgcttccgtt caaacgataa cttttccgac
      541 catccgaaca ctcaagaagc gttgtgccat agtgcagcac gaaagtacct tcttgttctg
      601 cttctaaacc cgtgatgcga ccaccatttt gcggctggcc aggataggct tcatctacca
      661 aattgatggc aatacaccat ctttcatagt cccattccac ctgcatcttg tgcaagtttc
      721 cctgtgcaca agctagcatt acttctagaa ggtcggattt tagcagcggt agaatcgcct
      781 gggcttctgg atcgccaaac cggcaattaa actccagaac ctttgggcct tccggagtaa
      841 gtattatccc agcatacaac acgcctcggt acatcgcgtc ctgccaaagg agttcggcaa
      901 ccatcggcct catgattgtt tccataattt cttcttcaag gccaaggttc caaaatggag
      961 ctggagcaat tgctctcatg ccgcccgtgt tcggattgtg ctcgcgtttt tcgcgttctg
     1021 cgaaacggag tttccggtcg cgcgccgaga aaggcctaga aacacgcctg ccgtcggtaa
     1081 acacaaatac actcacttcg ggcccttcaa gaagctcctc gagaagaaca actcttccag
     1141 cgttgttaaa cgctccctct tcgaggcagg caaaagccgc agcccatact tcttccacgg
     1201 ttttgggaac cgtaactcct ttgccgccag tgggtttgtc tggctttacc actactggct
     1261 ggtttccgtt ttccgcgagg caccgctcta cgtctgccat actgcgagca acccaagctt
     1321 tgggcctcgg cactcccctt ctccgtgcta ggcgatcgga ctccactttg ctgccttccg
     1381 tcttaaaggc tgcttttgcc cgagggccaa atgtaggaat tccgactgtc aacaacttat
     1441 cggcaactcc gtttataagc ggatcttccg gccctacaac cacaagcccc acgccaagcg
     1501 ctgccgccag cttcactacc tgatcttcga aggtgtcttt cctgtctgtt ctcaatacta
     1561 tattctcggc gacctgcgct gtgcctgcat ttccacgaaa acaataaagc tttcgcagca
     1621 aggggctctc cgcgagtttc aggcacagca tgttcgtacg agcatcttcc cctactacca
     1681 aaacatctat actatccatg atctttcctc tccagaagta aaaatgaaaa catacgcaaa
     1741 aagcgcatgt gctataggta ggatagcaaa ttttagcggg ttggcaaatt agtccttgcc
     1801 gcccttgtac gtgttgcgct tggcgaggtt ttgagctcta gtggggcgct tggcctgggt
     1861 ttttttggcc tcgtttgctt tttgtttgta ttgaaaactc gaaggcgtac gatatgttac
     1921 cctgtttttc atatacttaa ttgtactaca atgcgcctcg caagggaaaa ggccaacttg
     1981 acgaatattc tcttccccta tatggttaca gaagagttag ccagcacata ccacattctg
     2041 gcacaaggaa ggaaagaaaa tggcaaccat agttcttgag ccgcgacagg caaatctaaa
     2101 aatattatgc attggtggcg ggccagagat ggctaaaacc atctccgcat gcatgcagaa
     2161 cccgtgggtg aaagttatag ttgatagtgc cctaacggct gaagaaggac ttcagaaagc
     2221 gggccaaaat cctccgcact ttgtcgttat gaggcccgac tttacggatg caagccttgc
     2281 cagggcgatt ggggagctaa aaagcttttc gtcgacccct atacttgttc tcggttacca
     2341 cggaagcgca gcagaagaga tcgatgcttt ggcggaagga gcagatgagt ttgtctcctt
     2401 tcctcctcgc gatttgaagg ttttagagat caggatatgg aatgtgctcc ggacaagggc
     2461 aggtatgttc ctacaaccag tagaagagat ggccgctctc acaagcggag aactgatgct
     2521 caatcccgga gcttgtgaaa tttccctgaa cacgaaacgt atgcagctca ctcctacaga
     2581 gtttcgagtt ctgtacctcc tcatgagaaa ccgaggaaat gtgctctctc accaattttt
     2641 cggagactcc atctggggag acgaagtgga tagctcgggg ctggtaaaaa agtatatcca
     2701 gaaattaaga aggaagttgg gagacgacgt cgaagaaccg cgctggatag acactgtgcc
     2761 cagggtgggg taccgatttg taggccctgt tgtacgggct attcccccga tgccgcctct
     2821 tgcaaccttc gaagacctaa ctgaagaccc gcctccggat caacggagcc tcatggcgcc
     2881 cgtgtaataa aacaaaacag gagtttgcct gtgcaactcc tgtttttatt ttgtgtatgc
     2941 gtactagata tccttttgga aactttgata atagcttgcc gcggtgttca aacacacttg
     3001 ttgcgtgtaa tcgctaaacg cgttgcataa ctgttttgct tgaacatccg aatggaaata
     3061 cgaaataaaa tcttttacgg cgccgattac acagtttgtc tgctgccttt catctgtacc
     3121 cagcaagcag tatgctttcg tgcgcgcaac gtcgcttacc gtggaacccg aagcgtctcg
     3181 ccccacgctt tggtagcagg tatctttatg ctgctcctcg atggtggaac actgcacaaa
     3241 agcatcggca aagttgtatc catttacctt aagcacatac gaagtttgca ttaagtagca
     3301 cgcgtgtttg ttcttttctg gtacagcagt acacgggtac aaaggttctt cgggttttaa
     3361 gtatttggta aaatgatttt caaaatcggt tatgacgttt tccatgaaca cgcccccgta
     3421 acatgattct ttttcccatc cgccctgcag attgtcgcac agcgccaatg cctcaaacaa
     3481 ctcgctttgc gtaatataca tgacgccatg gcccaagcca tgcacgcagt tgtagtagtc
     3541 aaaagaatag ctctctctcc ccggaatgtt ctggcaaata gagtttatca ctcctggcaa
     3601 atcttcgggc gcggtttcgt acaatatgcc ttccattacg ccgtgatagt atccagacca
     3661 gcagaagtgg tccccttttg tatacgcttg tgatacatcg gggtatttct gggtggcagc
     3721 ccggcctata acatgaacta acggatggca aaacgcgcgc acggtg
//
LOCUS       DASRUP010000058         6965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_194406, whole genome shotgun sequence.
ACCESSION   DASRUP010000058 DASRUP010000000
VERSION     DASRUP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194406"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VFE94_02290"
     CDS             <1..1427
                     /locus_tag="VFE94_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZX49960.1"
                     /translation="ELERQYSKNEILGFYLNQIPFGSNAYGIGAAAELYFAKKPADLT
                     LPESATLAALIQAPSYYSKNKDVLDTRKNHVLNRMAEEGFLTPQEADEAKTAEVAFQD
                     VDSTIRAPHFVLSVIDELMKKYGEQFVRENGLRVTTSLDWELQNAAEQAVQNGAARNI
                     SSGAHNAALVALNPQTGEILAMVGSKDWNADSEGCTQEKGCLFDPKVNIATWGQGRQP
                     GSAFKPFVYAVAFQKGASPETTVMDEQTNFGVWGGKEYIPQNYDGKWHGEVTLREALA
                     QSLNIPAVKVLLNFAGIPDSIEFAKKLGITTLKDPSFYGPALVLGGGEVRLLDMVSAY
                     GVFAADGKKTPPISILKIEDAQGNVLETNQNTSIRVVSPGITQQVTSILSDNEARSPV
                     FGPNSSLYIPGYQVAAKTGTTQEYKDAWAIGYTDYIVAGVWAGNNNGTPSIRDAAVTL
                     AAPIWNQFMRQAIPHAQLLSTATL"
     gene            complement(1417..2544)
                     /gene="dnaN"
                     /locus_tag="VFE94_02295"
     CDS             complement(1417..2544)
                     /gene="dnaN"
                     /locus_tag="VFE94_02295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZX49961.1"
                     /translation="MKTVVLKEKLKEGLEVTERVAVKSSSLPILQNVLLGVEQKRARL
                     SATDLQMGVLYEFLATTEKEGQVVFPARFLYSVLGISKEDRVELEVKNKELQVVIGQQ
                     TLSIKTLDPEDFPIIPSVKGDETSFEVETSVLCKGLAQVVGMVGQSQVRPEISGVFFV
                     FSKKELRVVATDSFRLAEKTFQLEKEGVPEQSFILPAKTARELIATFAERPGKTQIYL
                     SPTQAIFDYMAKDDPSQVRLQIVSRLIEGEYPRYEDVIPSSHKTRLLVEKEAFIAYVK
                     AAGVFAGKTSEVRLSVEPAQKSVEFFSHAPDVGEQRSQLSCEVQGAGVEVAFNWRFLL
                     DGLLQLKGNEVEFLLNGEDGPALLRQGEQQGYRYVIMPIKA"
     gene            complement(2720..4078)
                     /gene="dnaA"
                     /locus_tag="VFE94_02300"
     CDS             complement(2720..4078)
                     /gene="dnaA"
                     /locus_tag="VFE94_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZX49962.1"
                     /translation="MSKEELWQAVLAQLQFQISPANFATWLKNTFILSQKDGQLIISV
                     PNNFSKEWLENKYNKTIFHIIRSLDAEIKEIKYAVGTPPQPRQSSPARPSFIPLEESQ
                     LEFQELKIDRSTNLNPKYNFDTFIVGPFNELAHAASLAIIKTPGTIYNPFFIYGGVGL
                     GKTHLLSAIGNQISSDFPDKKVRYMSSERFTTGVVSAIRNHTMDAFRSLYRELDVLII
                     DDIQFLAGKDKTQEELFHTFNTLYEKGKQVIFSSDRPPKSIPALEERLRSRFEGGMIA
                     DISSPDFESRMAILKTKIQGYDMEMPDDVCEYVVAHIQRNIRELEGALNRLFAFYKFN
                     HKAPTLQEARSLLRTVIQNPNKVVSPKKIIQIVAEFYDLREKEIVAFSRKKEVVRPRQ
                     IAMYLLREELKSSYPLIGQKFGGKDHTTAIYACEKIGRELQESEVLIDELNLIRQRIY
                     SG"
     gene            4269..4403
                     /gene="rpmH"
                     /locus_tag="VFE94_02305"
     CDS             4269..4403
                     /gene="rpmH"
                     /locus_tag="VFE94_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZX49963.1"
                     /translation="MSITYQPKKKKRKRTHGFRKRAKTTGGSRTLQRRRRKGRKKLAV
                     "
     gene            4407..4748
                     /gene="rnpA"
                     /locus_tag="VFE94_02310"
     CDS             4407..4748
                     /gene="rnpA"
                     /locus_tag="VFE94_02310"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZX49964.1"
                     /translation="MLPSRHRLKRTKDFKGLATKRPGVRVSGLLLKTGTSRQEATRFG
                     IVVSKKVAKRAVERNLIRRRLRAAIREELPEVKPGLNVALIVLPGFNLQGAEEMQKSL
                     HKLFEKASLFQ"
     gene            4753..5496
                     /locus_tag="VFE94_02315"
     CDS             4753..5496
                     /locus_tag="VFE94_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZX49965.1"
                     /translation="MDFFIAIYELFLYQPIFNLLIFFSQNIPGQDFGVAVILLTLAVR
                     IVSYPLGAQAVRAQKRFAELQPKIKEIQEKFKNKKEEQTKAILEMYRTSKVNPFATFA
                     PFLMQIPIFIVLYHIFSKGIHVDQLHLLYSFVSVPPMVEVSFLGVLNLNEQSFALALV
                     AGVLQFVQLKQASPSSKRKKGSKPDIASLMQRQMPYIFPVLLVWVAANLPSAFGLYLA
                     TTTLFSIWQHWFITRKDHAHASETTTPNN"
     gene            5465..5935
                     /locus_tag="VFE94_02320"
     CDS             5465..5935
                     /locus_tag="VFE94_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HZX49966.1"
                     /translation="MLQKQQLQTIEQEARRFFEELGDIETVSIEQKEDEEEATVSVLV
                     KAADPKILIGERGQTLFEIQHLLKLIVRRKIAEPFYLSLDINDYKKNKEEYLRDLAQN
                     AADEVVLLKKPKELPPMAAAERRIIHTALNQRGDVVSESAGEGLDRHVVISLKS"
     gene            complement(5944..6822)
                     /locus_tag="VFE94_02325"
     CDS             complement(5944..6822)
                     /locus_tag="VFE94_02325"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HZX49967.1"
                     /translation="MRTLFVFSIIALGIVVPLLSSAQGIQLNLDYPVFGNIDLNTNQN
                     INAIIGWLYYAIVGISGLAAFVMLVWGGVQWLVSGAMPSQLGEAKDKLRAAILGLLLV
                     LASFLIMQIINPELTNLNQPALQRFQDSVVPTDGVQGVSPQCSDGKDNDGNGDVDLAD
                     PDCANDPNGTSEGIAGGGSVGGGAVGGGTPGGGTPSTGACSPPVDPNLNIVNPLDPTT
                     VGFIYGVGDPVLHNGVSKCLQGFNRSGLLGRVQIFFTDGSRVIASSSDPDGRNLAPTI
                     TNPAGTIDLLGNPVAP"
BASE COUNT         1836 a         1674 c         1713 g         1742 t
ORIGIN      
        1 tagaactcga acgccagtac tccaaaaacg aaatccttgg cttttacctt aatcagattc
       61 cgtttggttc caacgcctac ggcatagggg ccgccgccga actgtacttc gccaaaaaac
      121 ccgcggatct cacgttgcca gaatctgcga cgctggcagc ccttattcaa gctccttcgt
      181 actactcgaa aaacaaagac gtgcttgata ccagaaaaaa ccacgtatta aaccgcatgg
      241 ccgaggaagg ttttctcacc ccccaggaag cagacgaagc aaaaaccgca gaagtggcat
      301 tccaagacgt cgattccact atcagagcgc cccacttcgt gctttcggtc atcgacgagc
      361 tcatgaagaa atacggagaa cagtttgtga gagagaatgg cttgcgagta accacatcac
      421 tcgattggga actgcaaaat gccgcagaac aggcggtgca aaacggtgcg gccagaaaca
      481 tatcatcggg cgctcataac gcagccttgg tggcactcaa ccctcaaaca ggggagattt
      541 tggctatggt ggggtcgaaa gactggaacg cggactccga ggggtgcaca caagagaaag
      601 gatgcctttt cgacccgaag gttaacatag ctacctgggg gcagggaagg cagcctgggt
      661 ctgcgttcaa gccttttgtg tatgccgttg ccttccagaa aggagcgagc cctgaaacca
      721 cggttatgga cgagcaaaca aacttcggcg tatggggcgg caaagaatac attccccaaa
      781 actacgacgg aaaatggcat ggtgaggtta cgctgcggga agcactcgcc caatcgttaa
      841 acattcctgc cgtaaaagtt ttgctcaatt ttgccggcat cccagacagc attgagtttg
      901 caaaaaaact cggcattacc acactaaaag acccttcttt ctacggtccg gctctcgtgc
      961 ttggaggcgg ggaagtgcgc ttgctcgaca tggtttcggc ttacggcgtt tttgccgccg
     1021 acggaaagaa aactccaccc ataagcattc ttaaaattga agatgcgcag ggaaacgttc
     1081 ttgaaaccaa tcaaaacacc tccattcgag tcgtttctcc aggcattact caacaagtaa
     1141 cgagtattct ttcagacaac gaggcgcgaa gccctgtctt tgggcccaat tcttcgcttt
     1201 atatccccgg atatcaagta gctgcaaaaa cgggtacaac gcaggagtat aaagacgcct
     1261 gggcgattgg ctacaccgat tatatcgttg ctggcgtatg ggccggaaac aacaacggca
     1321 ctccgagcat cagagacgct gcggtaacgt tagccgcccc catttggaat cagttcatgc
     1381 gacaggctat tccccacgcc cagctccttt ccactgctac gctttaattg gcatgattac
     1441 gtaacgatac ccttgctgct ctccctggcg taacaacgcg ggtccgtctt ctccgttgag
     1501 aagaaactct acctcgtttc cctttaactg caagagcccg tctaaaagaa accgccagtt
     1561 aaacgcaact tccacccccg caccctggac ttcgcaagag agctgcgagc gctgttctcc
     1621 cacatctggc gcgtgcgaaa aaaactccac gcttttttgg gcgggttcca ccgacaaacg
     1681 aacttcactg gtttttccgg caaatactcc ggcggccttc acgtaagcaa taaacgcctc
     1741 tttctctacg agcaggcgcg ttttgtgaga agaaggaatt acgtcttcgt agcggggata
     1801 ctccccttct ataagccgag acacaatttg caagcgaacc tgcgagggat cgtctttggc
     1861 catgtagtca aaaattgcct gggttggaga aagatatatt tgcgttttcc ccggcctttc
     1921 cgcaaaggtg gcaataagtt cgcgcgcagt ttttgcgggg agaataaaac tttgttccgg
     1981 aacgccctct ttttccagct gaaacgtttt ctctgctaag cggaagctat cggttgcaac
     2041 cacgcgcagc tccttttttg aaaatacaaa aaacactcct gaaatttccg ggcgcacctg
     2101 cgactgccct accataccca ctacctgcgc caaccccttg caaagtacgc ttgtttcaac
     2161 ctcgaaagaa gtttcatctc cctttactga agggataatg gggaaatctt cggggtcgag
     2221 cgtttttatg gaaagggttt gttgccctat gaccacctga agttctttgt tttttacctc
     2281 cagctctacc cggtcttctt tggatatccc caacaccgag tacaaaaaac gcgcgggaaa
     2341 taccacttgt ccctcttttt ccgttgtcgc cagaaactcg tagagcactc ccatctggag
     2401 atcggttgcg gacaaacgcg ctcttttttg ctcaactcca agaagtacgt tttgcagtat
     2461 gggaagggaa gacgacttta ccgcaacccg ttcggtaact tctaaacctt ctttcaattt
     2521 ttctttcaac acaaccgttt tcatacacaa tatataataa gtaatatata tctttagttg
     2581 ttactgtacc tgtgaatttg tggaaaacgc tgttttggct ttaccagaaa ggcttttgtc
     2641 gccaaagaac tggggaaaaa aagagaacaa gcaaacggat aaaaaaagaa ctgaagttac
     2701 ccaccggttt tcccaagatt tatccactgt atattcgctg tcgtattaag tttaattcgt
     2761 caatcagaac ttctgattcc tggagctccc ggccgatttt ttcgcaggcg taaatggcgg
     2821 tggtatggtc tttcccgcca aacttttgcc caattaaagg gtaagaactt ttgagttctt
     2881 cgcggagaag gtacatggcg atttgccgcg gccgcaccac ctcttttttg cgtgaaaaag
     2941 cgacaatctc tttttcacgc aagtcgtaaa actcggcgac tatctgaata atctttttgg
     3001 ggcttactac tttgttggga ttttgtatta cggttcgcaa aagagaacgg gcttcctgga
     3061 gcgtgggagc cttgtggttg aatttataga atgcaaacaa ccggttcaag gcgccctcca
     3121 gttctctgat gttgcgctgg atgtgagcca ccacgtattc gcatacgtcg tctggcattt
     3181 ccatgtcgta gccctgaatt ttggttttta agatcgccat gcgcgactca aagtcggggc
     3241 tggaaatgtc ggcgatcatg ccgccttcaa aacgagagcg cagccgttct tctagggcag
     3301 ggatagattt gggagggcgg tcggaagaaa aaataacttg cttgcccttt tcgtaaaggg
     3361 tgttgaacgt atggaaaagt tcttcctgcg tcttgtcttt tcccgcaagg aactggatgt
     3421 cgtcgataat gagaacgtcg agttcccggt agagagaccg gaacgcgtcc atggtatggt
     3481 tgcggatagc agaaaccacg cccgtggtaa aacgctccga agacatatac cgcactttct
     3541 tatccgggaa atcggaagaa atctggtttc ctattgcaga aagcaggtga gtttttccaa
     3601 gccctactcc tccataaatg aaaaaggggt tataaatcgt tccaggggtt tttatgatgg
     3661 cgagagaggc ggcgtgagct aactcgttaa aagggcctac gataaaggta tcgaagttgt
     3721 atttggggtt taagtttgtg gaacggtcta tcttaagttc ctggaattcg agctggcttt
     3781 cttcgagggg gatgaaagag gggcgggcag gggaagattg cctgggttga ggaggcgttc
     3841 caacggcgta tttaatttct ttgatctccg cgtcgaggct gcggataata tggaaaatgg
     3901 ttttgttgta cttattctca agccactcct tggaaaaatt gttcggcacc gatatgataa
     3961 gctgaccatc cttttgtgaa aggataaagg tgttcttcag ccaagtcgcg aagtttgccg
     4021 gagaaatttg aaattggagt tgggcaagaa ctgcctgcca aagctcttct ttactcatac
     4081 cttgtaacaa gctttatgtt gatgattttc attataccat aggaaggagt aaggcaaaat
     4141 aaagggaaga gaaagagcct tgttgataac ctggggataa tatgggtata aatacccgtt
     4201 cttttcgccg ggaatacggc ccgattgacc gcaggggaat ttagaactat actgtatcat
     4261 ataggtctat gagtattaca taccaaccca aaaagaaaaa gcgaaaacgc acccatgggt
     4321 ttcgcaaaag ggcaaaaacc acgggaggca gccgtacgct tcaaaggaga aggagaaagg
     4381 ggcgcaaaaa gttagcggta tagcccatgt tgccttctcg ccaccggctc aaaaggacga
     4441 aagactttaa ggggcttgcg acaaagcgcc cgggggtccg ggtgtcgggg ctgcttctca
     4501 aaacagggac ttccagacaa gaggctacgc gttttggcat tgtcgtaagc aagaaggtgg
     4561 cgaaacgcgc ggtggagcgg aatctcattc ggagaaggct tcgggcggct atcagagaag
     4621 agttgccaga ggtgaagccc gggctcaatg ttgcgttgat tgttctcccg ggctttaacc
     4681 tgcaaggtgc agaggaaatg caaaaaagcc tgcataaact tttcgaaaaa gcctctttat
     4741 tccaatagca gtatggattt ttttattgcc atttacgaac ttttccttta ccagcccatt
     4801 tttaacctgt tgattttctt ttcccagaat atcccggggc aagattttgg cgttgccgtt
     4861 attcttttga cgctggcggt gcggatagtt tcctaccctt tgggggcgca ggcggtgcgc
     4921 gcgcagaaaa ggtttgcaga attgcagccc aaaatcaaag aaattcagga aaagttcaaa
     4981 aacaaaaaag aggagcagac aaaggcaata ctcgagatgt accggacttc gaaagtcaat
     5041 cctttcgcaa cattcgcccc ctttttaatg caaattccca tttttattgt gctctatcat
     5101 attttttcca agggcataca cgtagatcaa cttcaccttt tgtactcttt tgtttctgtc
     5161 ccccccatgg tagaagtatc ttttttgggg gtgctcaatc tcaatgaaca atcctttgcg
     5221 ctcgcacttg ttgcgggggt tctccagttt gtccagttaa aacaagcgag cccaagctca
     5281 aaaagaaaga aaggctcaaa gcccgacata gcttcgctta tgcaaaggca aatgccgtat
     5341 atttttcctg ttctattagt ctgggttgcc gcaaaccttc cttccgcttt cgggctctac
     5401 cttgccacaa ccacgctatt ttccatttgg caacactggt ttatcacaag aaaagatcat
     5461 gcccatgctt cagaaacaac aactccaaac aattgaacaa gaggcgcgcc gttttttcga
     5521 ggaactgggt gacatagaaa ccgtttccat agagcagaaa gaagacgagg aggaggcaac
     5581 ggtgagtgtt ttggtaaaag cggcagaccc caaaattcta atcggagaaa ggggccagac
     5641 gctttttgaa atccagcacc tgctaaaact cattgtgcga agaaaaattg cggaaccttt
     5701 ttacctttca ctggacatta acgactacaa gaaaaacaag gaagagtact tgcgcgatct
     5761 tgctcaaaat gccgcagacg aggttgtatt gcttaaaaaa ccaaaagagc ttccgcccat
     5821 ggcagctgcc gagcggcgca ttattcatac cgcactcaac cagagaggcg acgtggtgtc
     5881 tgaaagcgcc ggtgaaggtt tagaccgcca cgtggtgatt agcctaaaaa gttagacttt
     5941 agactaggga gccacggggt tgcctaaaag gtctatggta ccagcggggt tggtaattgt
     6001 aggagcaagg ttgcgtccat cagggtcgct cgaagaggca atgacacgcg aaccatcagt
     6061 gaaaaatatc tgcactcttc caaggaggcc gctacggttg aatccttgaa gacattttga
     6121 gaccccgtta tgcaacacgg gatcacccac gccatagata aatcctaccg ttgttgggtc
     6181 tagagggttt acgatgttga gattaggatc tacgggaggg gagcatgctc cggttgacgg
     6241 cgtgcctccg ccaggagtcc cgcctccgac ggcacctcct cctacactac cacctcccgc
     6301 aattccttcg cttgtgccat taggatcgtt agcgcagtca gggtctgcca ggtcgacatc
     6361 tccattacca tcattgtctt taccgtctga acattggggg gaaaccccct gcacgccgtc
     6421 tgtgggaaca acactgtcct ggaatcgttg cagggccggc tggttgaggt tggtgagttc
     6481 gggattgatg atttgcatga taaggaagga ggcgagcacc aaaagaaggc cgagtatggc
     6541 agcccgcagt ttgtccttgg cttctcccag ctgacttggc atggctcccg acactaacca
     6601 ctgcacgcct ccccatacga gcatcacaaa ggcagcaagg cctgaaattc ctactatggc
     6661 gtagtacagc catcctatga tggcgttgat gttctggttg gtattcaaat caatgttgcc
     6721 gaatacgggg tagtctaagt tcagctgaat gccctgggcg gaggaaagga ggggaacaac
     6781 gattcccagc gcaattatgg agaaaacaaa tagtgttcgc attgccttat tatataaaag
     6841 aaacagtgtc cagtacaact atcgccaacc gagttttcca ctcaagcttg ctacatgctc
     6901 tgtatcgagc gtgtcttgta cctcgggata tttttcaagc tcacgggcaa gaatgttttt
     6961 gattt
//
LOCUS       DASRUP010000059        16129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_701252, whole genome shotgun sequence.
ACCESSION   DASRUP010000059 DASRUP010000000
VERSION     DASRUP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16129
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701252"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1013
                     /locus_tag="VFE94_02330"
     CDS             36..1013
                     /locus_tag="VFE94_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZX49968.1"
                     /translation="MARRLVLFLATLGVGALFFVLLISKLGWEEIAKELSSFSLSMAL
                     VVALLTAIFLLAGAFRWKQVLLSLGSPVSLSKLWGPYLAGFSLLFFVPVIPFASELFR
                     AGVLQKQHRIPFSPSMASVVIDRILEITSNLSVIILGGLVFLAVGRPAFLSLKMAGIM
                     VFVSLWFVFLFVLYARIFQRKSVLSLLWRNGSRAYEVEQEVFRFFRLRNAFFWRGLGA
                     SLLRSLAGYARAVAILFALGKGFAFLPGVAVLGFYYLGVLVPVPAALGSHDALQAVAF
                     NTFGFGAGAGAAFALVIRSIEVVFALGGLWLLAQFGFSIAKTILLKHGS"
     gene            1010..2737
                     /gene="polX"
                     /locus_tag="VFE94_02335"
     CDS             1010..2737
                     /gene="polX"
                     /locus_tag="VFE94_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HZX49969.1"
                     /translation="MNKELAAVFSRMAMYLQIDEVPFKPQAYERAAEALEVLSESVKE
                     VYKKGGKKGLEEIPGVGKGIAEKIEEYIKTGSVREYEQLRKKIPVDMEELTRVEGLGP
                     KMVRDLYKHLKIKTLRDLERAAKAGKIRDLPNFGEKTEQNILQGIAFVKKDTGRWLLG
                     KIYPSMELLVAELQKSPAINQAIAAGSIRRMKETIGDIDILATSSKPEKAVEYFLSKV
                     SYEKIWGKGATKVSVRTKEGFDVDLRILPDEVFGAGLQYFTGSKEHNVKLRTYAASKG
                     YKLSEYGLFKGKKRIACRTEEEVYKALGMAYIEPELREDQGEIEAALRQAQGKLPGLP
                     NVIPYGSLKGDLQIQTDWTDGKHSIENMAKEAKKQGLEYIAITDHTRDLAMTGGLDEK
                     KLELQMKEIDEVNKKVSGVRILKGAEVNIRKDGSLDIADEALAKLDVVGISVHSNFKM
                     NKKDMTERIARAMQNPHADILFHPTGRLIHKREAYDVDMDKVVQKAKETGTVLEINAF
                     PERLDLKDVDIKKAKEAGVKFAIDSDAHATEHIAYLKYGVAQARRGWAEAKDVINAWP
                     REKMLSMLK"
     gene            2742..3200
                     /locus_tag="VFE94_02340"
     CDS             2742..3200
                     /locus_tag="VFE94_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZX49970.1"
                     /translation="MAKEKSAGAIVFRIDKGEPLYLLLHYPSSVKAKREYWDLPKGHM
                     EKGESEEETVRREVQEETGIQDIEIFEGFRTTIHYWFEAKGQKISKTVVFYLAKTKEQ
                     RIVISTEHEGFKWLPYKEALAQLTYKNAKEVVQKAHRFLGGRDAIPKKGV"
     gene            3172..3426
                     /locus_tag="VFE94_02345"
     CDS             3172..3426
                     /locus_tag="VFE94_02345"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZX49971.1"
                     /translation="MTPFQKRVYEAVKRIPRGEVRTYKQIAQAIGCPKAYRAVGNALN
                     KNPDLKTIPCHRVVRSDGKIGGYRFGSSKKKTLLLREGAI"
     gene            complement(3419..3763)
                     /gene="rplT"
                     /locus_tag="VFE94_02350"
     CDS             complement(3419..3763)
                     /gene="rplT"
                     /locus_tag="VFE94_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZX49972.1"
                     /translation="MSRVKRGTTASKRRKNVLQKTKGFRWGRKTKYRLAKDALRHAWE
                     YSFRDRKAKKAVFRQSWQQSIQAAAKAHDISYSRLIAGLKTQNIQLDRKVLAELAKTN
                     PEAFGKIVAKVK"
     gene            complement(3766..3963)
                     /gene="rpmI"
                     /locus_tag="VFE94_02355"
     CDS             complement(3766..3963)
                     /gene="rpmI"
                     /locus_tag="VFE94_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZX49973.1"
                     /translation="MAKQKTRKSITKRFKITKSGKILRRLVGQDHYLAKQSSKKRRNM
                     RKWVELSTPETKAIKRLLNSK"
     gene            complement(3965..4474)
                     /gene="infC"
                     /locus_tag="VFE94_02360"
     CDS             complement(3965..4474)
                     /gene="infC"
                     /locus_tag="VFE94_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZX49974.1"
                     /translation="MQKRIYTNNQIRANQVRLIDETGKQLGVVPLQEGLQLARERNLD
                     LIQVTEKVEPPVCKIGEYGKYLYQLEKKAREIKKPEGGELKEVRLTFTISEHDLETRV
                     KQAEKFLQKGHRIRLTMRLKGRQNALEGYAREKVQKFLSALEQKTPVKMERELKKEPR
                     GLSAIIAKK"
     gene            complement(4504..4855)
                     /gene="ssrA"
                     /locus_tag="VFE94_02365"
     tmRNA           complement(4504..4855)
                     /gene="ssrA"
                     /locus_tag="VFE94_02365"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4844..5296)
                     /gene="smpB"
                     /locus_tag="VFE94_02370"
     CDS             complement(4844..5296)
                     /gene="smpB"
                     /locus_tag="VFE94_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010477042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZX49975.1"
                     /translation="MAVYAENRKAHFDYEILETFQAGLALQGQEVKSIQNGRIQLAGS
                     YVVFRGEELFLIGAHIPPYQPANAPADYSAERSRKLLLRKEELRYLLGKGKEKGLTLV
                     PLSVYNTKRKIKLEFGLARGKKKADKREDIKKRETKREIERALKHGDV"
     gene            5531..6250
                     /locus_tag="VFE94_02375"
     CDS             5531..6250
                     /locus_tag="VFE94_02375"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZX49976.1"
                     /translation="MSYNKGTRAKKTIGILGGMGPLASANLYLKMVKASQEMYHAEQD
                     TDFPPIFIYSLPLFGFDQTGFVDQALVRNQLLSGVKKLEEAGSDFIVIACNTVHYFYE
                     DMQNAVKIPIVSIIEETSRVAQEEGYKTIGLLSSESTNALGIYQKDLARRGVKVLSVD
                     KRQQELLNKVIFSVMSGIQGVEEKEILKEVMDDLCGQGAQAIILGCTEIPLAIQQSDV
                     DVKVFDSTEIITQCALERAME"
     gene            6302..8014
                     /gene="argS"
                     /locus_tag="VFE94_02380"
     CDS             6302..8014
                     /gene="argS"
                     /locus_tag="VFE94_02380"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZX49977.1"
                     /translation="MTIKQEIEKLIREAVRGFGKVGEIPLEHPENPDHGDYATSVAFA
                     LAKQLGKNPQEVAKEIASKFDIQNSKFLDKVEVVGGFVNFYLSAKVFEATLQAVLKEE
                     EKFGKGSVLKGKNIMVEYAHPNTHKEMHIGHMRTLVTGEALCRIFEAAGAKVFRANYQ
                     GDVGPHVAKAIWGTEKLLAERDTSWEDAEKLSLSEKAHLLGQGYVKGNQEYENSKQEI
                     DALNKKLYQQDKEVAGVYERTRKWSLDYYDSFYKRFDTKFNKLYFESQVAGLGKSIVL
                     KNIGKVFEESQGAIVFKGEQYGLHTRVFVTQDGNPTYEGKEMGLAPTQHKDFPFDLNI
                     HVVANEQAGYFQVVFKALELIDEKFKGKEYHLSMGMVQLVGKKMSSRTGVLVTVDGLL
                     DDVKELLAQLITSEGFTPQEKEEVKETGTVAAVKYSVLKTDTKANVLFDLARSVALDG
                     NSGPYLLYTYARCKSILRKEKASNKQIKISNVAKEELDILRLMYRFPEVVQEAAEKYA
                     PNLVCAFAFDLSQKYNAFYNAHSVLQADTPEQKEFRLQLCAACAQLLQNSLHLLGIQT
                     LEKM"
     gene            8057..11518
                     /locus_tag="VFE94_02385"
     CDS             8057..11518
                     /locus_tag="VFE94_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZX49978.1"
                     /translation="MEADFPKLEEHILKRWKEQKAFEKSLERRKKAPRFVFYDGPPTA
                     NAPPGFHHLLSRSMKDVVLRYRTMRGFFVARKAGWDTHGLPVELQIEKKLGLQSKKEI
                     EKYGIAKFNKACRDSVWEFIQDWRNLSERTGFWIDMDNPYITYNTSYIESLWFVIRQF
                     AKKGLLYKDYKVVPYCPRCGTPESSHELAQGYKTVKDRSVYAKFKIKGEPSTYFLAWT
                     TTPWTLPGNVALAVGEKITYAFVEQKGETYILAKSLLNKLEGEYKVIKEVLGKDLEGL
                     EYEPLFDSLQNQPEKKHYVAQASFVSTDEGTGIVHTAVMYGEDDYQLGKKLSLPKKHT
                     VNEEGRFNELVPQWKGLFVKDAEPLIIEDLKERNLLYREELYEHEYPFCWRCGTHLLY
                     YAKDSWFINMQKVKKDLLANNESINWIPAHLKNGRMGEWLREVKDWAFSRERYWGTPL
                     PVWVCDSCGHQDVVGSIQDLQEQTSVGNTYYLLRHGDSARQKLQIASAWPEKKPLPLT
                     ADGIKEAGKAAKQLKAKKIDMIFSSDLLRTRETAEIVAKEIGREVHYDERLRENSNGI
                     FNGKSIEKVGAFYRQKGETMAEHYMRRFHMAPPEGESWTDVQKRTLDFICEMEKKHQG
                     KNILVVGHSLPLALFEGLLAGWSKQEIVEHFAKNKAPKLASTGEWRKVAGSRLPHNDE
                     MDVDLHRPYIDAIDFACKECSKGTRKRVKDVVDVWFDSGAMPFAQDHWPFEQNKKLTP
                     PKFYPSDYIVEAIDQTRGWFYTLLATSTLLGLGSSFKNVISMGHILDEKGEKMSKSKG
                     NIVNPWEMIEKYGVDTLRWYFYTFNQPWDSKAFVERDLQQTMRKFMLTLWNSLVFYQT
                     YATKPTKAASANVLDKWIISRLHSTSREAAKRFDEYDITGTARLLESFVIDDLSLWYI
                     RRSRQRFQNPKSKKELQDASSTLYLVLLEISKLLAPFVPFLAEHIYNELDARKQSVHW
                     QDYPKADAKKINAKLEKEMQAARDMVTKALAERAKAGIKVRQPLAALKFSMSNFQFSK
                     DVTDLIRDEVNVKEVMPVKNLKTEVELDTNITPALQREGTVRELLRHIQEMRKQAGYR
                     PQDRVRLRYAGEEQLKEILRENKQMVMKTVGLKEMLEGDRPKRVFDVEQEFLLDNQTL
                     WLGIRKL"
     gene            11555..11866
                     /locus_tag="VFE94_02390"
     CDS             11555..11866
                     /locus_tag="VFE94_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZX49979.1"
                     /translation="MRKEILYDKNALKELREFREEVQEEFAACIKTLKQEGQLEYPEA
                     RKVARNLFEIRVVHEGAYRAFYAYVMKESIVILHFFQKKTQKIPLKNLKLAQQRLKQY
                     E"
     gene            11859..12176
                     /locus_tag="VFE94_02395"
     CDS             11859..12176
                     /locus_tag="VFE94_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX49980.1"
                     /translation="MSKKYPRKIQQAIERGDLIPYEEVWRSYSKKQREAIEERARYLK
                     AAMELRKVRKQMHLSQEELAKKMSVKREFISRIESGRQNVTLDTLYRIAEVTGKEFRL
                     SFR"
     gene            12232..12918
                     /locus_tag="VFE94_02400"
     CDS             12232..12918
                     /locus_tag="VFE94_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49981.1"
                     /translation="MAEKFPKFKPAMRGEIVMGTEVATDLYYDLDGQLLTIKEQLRKK
                     GGYRFYPLQLADHLYAAIEGRFMSRDGRPLLVEPVFSPIPGDGEEFELILRDKVDPME
                     MVRNSGCDPDDWKFRGEQIIVPSIHHFKLVQVGYCRDFTEVQDKLTSHGNPANGWWRE
                     VFQKTYPRSDGKGPIGFLDHSWIDPAGRTPFPGLVCSRGHGRHYWTPRFFYVQRLRSY
                     NWRWLVEVEE"
     gene            13012..13635
                     /gene="recO"
                     /locus_tag="VFE94_02405"
     CDS             13012..13635
                     /gene="recO"
                     /locus_tag="VFE94_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZX49982.1"
                     /translation="MARYYRTQGIVLEKEDRGEADRVFTVFTKDFGKLRLRAVSERKI
                     TSKLRGGLELFYLAEIEFVQGKTQKTVTDAWAIDQHILLRRDVNRLRVMHRLAEIVDD
                     LVKGQEEDQRLWSLLSETVAFLNRFDMKAREVRLVSYYFLWNLFATLGYRPSLGPIAE
                     RNEKVAAVIRTFLQGDLAALQDLSKAGIQEDLLQKICQEHLSKVQES"
     gene            13649..15319
                     /locus_tag="VFE94_02410"
     CDS             13649..15319
                     /locus_tag="VFE94_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49983.1"
                     /translation="MRVKFVFASLFATVALAALGFWFWQQNTFSTGSLKLEILAPEEI
                     TMGQEITYVVRWKNNGETELENVELVFEFPEGTVLAQGGELRIIKQLDDVNSGQEGSE
                     RFVGRIFGKENELKEAKAFLTYSPKNLSASFQNETKTSSEISFVPINFELDIPSRIES
                     GQQFTVTLNYFSNSDYPLSDLRIQLEYPAGFEFRGANPAPIGENEWDIGLLNKAEGRR
                     ITITGAIQGDVQQVKNFKATFGSWKEGRFTVMKEVTKAMEITKPRIFITQVVNSSPSY
                     VASLGDTLHYEVFFRNPGDRILENLFLIVTLEGRAFDLQSVKAQNGRFQSGDNSIVWE
                     AKDVPKLRFLGTGEEGKVEFWINVKDEIDTASPQETELVLKDRVLLSEATEEFTVKLN
                     TGLTIEQKGFYQDEVFGNSGPIPPKVGEKTTYTITWQVKNRYNDIRNARVKAILPARV
                     ELTGEIFPEDAILTFDSGSREVVWEVGDVPAGSGYFETVPAQSVAFQVAFTPTSVHRG
                     DVVDLIREVRVTGEDVFTDQVVSSSDGAIDTTLPDDDSVDEDDGEVRG"
     gene            15378..>16129
                     /locus_tag="VFE94_02415"
     CDS             15378..>16129
                     /locus_tag="VFE94_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HZX49984.1"
                     /translation="MEDLMKKIVSLAKRRGFVFQSSELYGGVEALWDFGPLGAALKEN
                     IKKAWMRRFVQQMPNVVAIQGSIIMHPDVWKASGHLENFTDPLVECKKCNARLRQDHI
                     EDGKCPNCGSKEFSEARNFNLMFKTFLGPLEDEGHTAYLRPETAQSMFTNFKLVQESM
                     RQKIPFGIAQTGKAFRNEITTGNFIFRSREFEQMEIEFFVKPGQDDKWFDFWIDAWEK
                     FYLDLGIKKENLRRYEHPKEKLSHYSKRTVDL"
BASE COUNT         4548 a         3364 c         4195 g         4022 t
ORIGIN      
        1 tcgttttgag gatttgtgct ataatacacc atcttatggc tcgacgcttg gtactctttt
       61 tggcaacgct tggggtgggt gctttgtttt ttgttttgct tatctcgaag cttggctggg
      121 aggaaatcgc aaaggagttg agttcttttt ctttgtccat ggcgcttgtt gtcgccttgc
      181 ttacggcaat attccttttg gccggggctt tcaggtggaa gcaagtgctt ttaagtcttg
      241 gcagtccggt ttccttgtcg aaactctggg gtccgtatct ggccgggttt tcgttgttgt
      301 ttttcgtgcc ggtaatcccc tttgcaagcg agcttttccg cgctggggtg ctgcaaaaac
      361 agcaccggat tcccttttct ccgagcatgg cctcggtggt tatcgatcgc attctcgaga
      421 ttacctcgaa cctttcggta attattctcg gcggtttggt gtttttggcg gtgggcagac
      481 ccgcctttct ttctctcaaa atggccggta ttatggtttt tgtttccctg tggtttgtgt
      541 ttttgtttgt attgtatgcg agaatttttc agagaaagag cgtgctgtcg ctcctgtgga
      601 ggaatggcag cagggcgtac gaagtagagc aggaagtgtt tcggtttttc cgcttgcgca
      661 acgctttttt ctggcgcggt ttgggagctt ctttgcttcg aagcctggcg ggatatgcga
      721 gagctgttgc catacttttt gccctgggca agggttttgc ttttttaccc ggcgttgctg
      781 ttttggggtt ttactacttg ggagttttag tgccggtacc tgccgccttg ggctcgcacg
      841 acgccttgca agcggttgct tttaatacgt ttgggtttgg ggcaggagcc ggagccgcgt
      901 ttgcgcttgt tatccgttcc atagaggtgg tatttgcgct tgggggcttg tggctgttgg
      961 cgcagttcgg gttttctata gcaaagacaa ttttattaaa gcatggatca tgaataaaga
     1021 gttagctgca gtcttttctc gcatggctat gtacctccaa atcgacgagg tgccttttaa
     1081 gccccaggca tatgaaaggg cggcggaagc gctcgaagtt ctttcagaaa gcgtaaagga
     1141 agtgtacaag aaaggcggca aaaaggggct cgaggagatt cctggcgtgg gcaaaggcat
     1201 tgcagaaaag atagaggaat atataaaaac aggaagtgtg agagagtacg aacaactgcg
     1261 aaagaaaatt cctgtagaca tggaggaatt aacgcgcgtg gaggggcttg gccccaaaat
     1321 ggtgcgcgat ttgtataagc atttgaaaat taaaaccctg cgcgatttgg aaagggcggc
     1381 aaaggctggc aaaatacgag acctcccgaa ctttggcgag aaaaccgagc aaaatatttt
     1441 gcagggaatt gcctttgtca aaaaagacac ggggcgatgg ttgttaggaa agatttaccc
     1501 atctatggag ctgcttgttg cagagctgca aaagtctccg gcaatcaacc aggcaatagc
     1561 tgcggggtct atacggcgca tgaaagaaac catcggcgat atcgatattt tggctacgag
     1621 cagcaagccc gaaaaggccg tggagtattt tttgtctaag gtttcgtacg agaaaatatg
     1681 gggaaagggc gctaccaagg tgtctgtgcg aaccaaagag ggtttcgatg tggacttgcg
     1741 catcctcccc gatgaagtgt tcggagccgg cctgcagtat tttaccggtt ccaaagaaca
     1801 caacgtgaag ctgcgaacgt atgcggcaag caagggatat aagctttcag aatatgggct
     1861 gtttaaggga aagaagcgca ttgcgtgcag aaccgaagag gaggtgtaca aggccctggg
     1921 aatggcgtat attgagccag agcttcgcga agatcaggga gagattgaag ctgcccttcg
     1981 acaagctcag ggtaaactcc ccgggttgcc gaacgtaatc ccttacggaa gtctgaaggg
     2041 agatttgcaa attcagaccg actggaccga cggcaagcat tccatagaaa atatggcaaa
     2101 agaggcaaaa aagcaggggt tagagtacat tgcgattacc gaccataccc gagatcttgc
     2161 catgaccggc ggattagacg aaaaaaagct tgagcttcag atgaaagaga ttgatgaggt
     2221 caataaaaag gtttcgggag tcagaatttt aaaaggagcc gaggtgaata ttcgaaaaga
     2281 cgggtcattg gatatcgcag acgaagcgct agcaaaactc gacgtggtgg gaatttcggt
     2341 gcactcaaac tttaaaatga acaaaaaaga catgaccgag cgcatcgcgc gcgccatgca
     2401 aaaccctcac gcagatatac tgtttcaccc tacgggcagg ttgatccata agagagaggc
     2461 gtacgatgtg gatatggaca aagtcgtgca aaaagcaaaa gaaacaggca cggtgcttga
     2521 aattaacgcg tttccggaac gattggattt aaaagatgtt gatattaaaa aagcaaaaga
     2581 agctggggta aagttcgcga ttgattctga cgcccacgcc acagaacaca tagcatactt
     2641 aaagtatggt gtggcgcagg caagacgcgg ctgggcagag gcaaaggatg tgataaacgc
     2701 ttggccccga gaaaagatgc tttccatgct gaaataagaa tatggcaaaa gaaaaatcag
     2761 ccggagctat tgtatttcgt atcgacaaag gagaacctct gtatttgctt ttgcattacc
     2821 cttcgtctgt gaaagcaaaa agagagtatt gggatttacc caaggggcat atggaaaaag
     2881 gggagtctga agaagagacg gtgcggaggg aagtgcagga agaaactgga atacaagata
     2941 ttgaaatttt tgaaggattt cgtaccacta ttcactattg gtttgaggcc aaggggcaga
     3001 aaatttcaaa aacagtagtg ttttatttgg caaaaaccaa agaacaaagg attgtaattt
     3061 caacagagca cgaggggttc aaatggcttc cttacaaaga agcactggcc cagcttacat
     3121 acaagaatgc aaaagaggtt gtacaaaaag cgcaccggtt tttgggaggg cgtgacgcca
     3181 ttccaaaaaa gggtgtatga ggcggtcaaa agaatcccca ggggtgaggt gcgcacctac
     3241 aaacaaatcg cgcaggcgat tggctgcccc aaagcctata gggcagtggg aaacgcgctc
     3301 aacaaaaacc ccgatctcaa aactattccc tgccaccgcg tagtgcgctc ggacgggaag
     3361 atcggaggct accgcttcgg ttcttccaaa aagaaaacgc tcctcttgag ggagggcgct
     3421 atttaacttt tgctacaatt ttgccaaagg cttcgggatt tgttttggcg agttctgcca
     3481 gtactttccg gtccagctga atgttctgcg tttttaagcc ggcgataagc ctgctgtagg
     3541 aaatatcgtg ggctttggcg gcagcctgaa tactctgttg ccagctctgg cggaacactg
     3601 ccttttttgc tttccggtcg cggaacgagt attcccacgc gtggcgcaaa gcgtcttttg
     3661 caagcctgta ttttgtcttt ctgccccaac gaaaaccctt ggtcttttgc aagacgtttt
     3721 ttctccgttt ggatgcagtg gttccgcgtt ttactctaga catggttatt tgctatttaa
     3781 cagccttttg attgccttgg tttcaggcgt cgaaagctcc acccactttc gcatgtttct
     3841 tcgcttcttg gaagattgct tggctaaata gtggtcttgc ccaacaagcc ggcgaaggat
     3901 tttccccgac ttggtgattt taaaacgctt ggtaattgat tttctggttt tttgctttgc
     3961 catgttactt ctttgcgata atagccgaaa gccctcgagg ctcttttttt aactccctct
     4021 ccattttgac aggagttttt tgctctaacg cagacaagaa cttttgcacc ttttctcttg
     4081 cgtacccttc gagggcgttt tggcggccct tgaggcgcat ggtgaggcgt atgcggtgcc
     4141 ctttttggag aaacttttct gcctgcttta ctcttgtttc aaggtcgtgt tctgaaatcg
     4201 taaaggtgag cctgacttct ttgagttcgc caccttccgg cttttttatt tccctggctt
     4261 ttttctctaa ctgatacagg tattttccgt attctccaat cttgcataca gggggttcca
     4321 ccttttcggt tacctgaatt aagtcgagat ttcgttctcg cgcaagctgt aagccttctt
     4381 gcaacggcac gacaccaagc tgttttcccg tttcgtcaat aaggcgtacc tggtttgccc
     4441 gtatttggtt gtttgtgtaa attcgcttct gcaaagtgtg ttgttgaata gtcgcgagcg
     4501 tcactgtgga gatggcgggc atgaccccgc atccagcaaa ggaaaggaat gtaatcttcg
     4561 agtgtagtcc agttggtaaa gagagagagc gcgagaactg gacaaaaccg ttctctcttt
     4621 tgacggtcct catagttcag tagctttcgg accttcaaaa aagctgctta tcccgatgaa
     4681 tgacacctgc ttcttttacc gggaatcgaa gagcagatgg cctacagctt attaggcgta
     4741 ggcaagagcc ggctgtgccg gtctaaacag gttggcagtt gtatgccttg gttttaaaga
     4801 gtgccttctc tactcgcata caaacctcga ttttgctgtc gagtcacaca tccccgtgtt
     4861 ttaaagcgcg ttcaatctcg cgtttggtct cccgtttctt gatatcttcc cgtttgtctg
     4921 ctttcttttt gcctcgggcc agcccaaact caagtttgat cttgcgtttc gtattataca
     4981 ctgaaagggg caccaaagtc aagcctttct ccttgccttt gcccaggagg taccggagct
     5041 cttctttgcg caataagagc tttcgcgagc gctctgcgct gtagtctgca ggagcgttgg
     5101 caggctggta ggggggtatg tgcgccccta ttaaaaaaag ctcctcgccc cggaacacca
     5161 cgtaggaccc ggcaagctgt atcctgccat tctggatgga ttttacctct tggccctgca
     5221 aagccaaacc cgcctgaaac gtttccagga tttcatagtc aaaatgcgct tttctgtttt
     5281 ccgcgtatac cgccattgct tcatttaagc actctgtttg ccgcttgaca actttgtccg
     5341 gctatagtat ctagtgagat atgcaacaaa caatccgtgc aagaagaact cgaagaaact
     5401 caaagaattt gaggttgttt gttgatgcgc gaagattaaa gtcataaatt tttttcgtct
     5461 gaactttcac aagcggcctc caacgaggac gcttgttttt ttaggacaaa ttcgcatgag
     5521 aagagaaaac atgagttaca acaaaggcac aagagccaaa aaaactattg ggatccttgg
     5581 ggggatgggg ccgcttgcga gcgcaaatct ttatctaaag atggttaaag cttcgcagga
     5641 aatgtatcat gcagagcagg atactgattt tcctccaata tttatatata gtcttccctt
     5701 gtttggtttc gaccagaccg gttttgtcga ccaagcttta gtgagaaacc aattactgtc
     5761 cggcgttaag aagctagagg aggcggggag cgattttatc gttatcgcat gcaataccgt
     5821 acattacttt tatgaggata tgcaaaacgc tgtaaaaatt cctattgtaa gtattattga
     5881 agaaacatcg agggttgcgc aagaagaggg ttataagacc atagggttgc ttagttccga
     5941 gagcactaac gccctaggta tttatcagaa agatttggcc aggcgaggcg tcaaggtgtt
     6001 gtcagtggac aagcgacagc aagagttact caataaggta atattttctg ttatgtctgg
     6061 cattcaagga gtcgaggaaa aagaaatact aaaggaagtt atggacgatc tctgcgggca
     6121 gggcgcacaa gctattattt tagggtgtac agaaataccc ttagcgatcc agcagagtga
     6181 tgtagatgta aaggttttcg atagcacgga gattattact caatgtgcgt tagaacgcgc
     6241 gatggaatag gcttgcttat agcttttatt ctagaggaaa atacgctaat gttacaatag
     6301 aatgaccata aaacaggaaa ttgaaaagct tatacgggaa gcggtgcggg ggtttggcaa
     6361 agtgggtgaa attccgttgg agcacccgga gaatccagac cacggggact acgctacaag
     6421 cgtggctttt gcgttggcaa agcaattagg aaagaacccg caagaggtag cgaaagaaat
     6481 tgcttcaaaa ttcgacattc aaaattcaaa attccttgat aaagtcgagg tggttggcgg
     6541 ttttgtaaac ttttacctgt cagcgaaagt gtttgaagcg acgcttcaag cagtgctcaa
     6601 agaagaggaa aagttcggga aagggagtgt tctgaagggc aaaaacataa tggtggagta
     6661 tgcgcatccc aatacgcaca aagaaatgca cattgggcat atgcgtacat tggttacagg
     6721 ggaggcgctt tgtcgcatat tcgaggctgc gggagccaag gtgtttcgcg caaattatca
     6781 gggcgacgtt ggcccccatg tggcaaaagc gatttggggt acagaaaagc ttttggcaga
     6841 acgggatacg agctgggaag atgcagaaaa attaagtctt tcagaaaaag cgcacttgct
     6901 ggggcaggga tatgtaaaag gaaatcaaga atacgagaat tccaagcaag aaatagacgc
     6961 gctcaacaaa aaactgtatc aacaagataa agaagtggct ggagtatatg aacgcacgcg
     7021 aaaatggagc ttagactatt acgattcttt ttataaacgt ttcgacacga aatttaacaa
     7081 actgtatttt gagagccagg tggcaggact tggcaagagt atagttttaa aaaacatagg
     7141 caaggtgttt gaggaaagcc aaggagccat tgtgtttaag ggcgagcaat acgggttgca
     7201 tacccgcgtg tttgttacgc aagatggaaa cccaacatac gaaggcaaag aaatgggttt
     7261 agctcccacg cagcacaaag attttccatt cgacctgaac attcatgtag tggcaaacga
     7321 gcaagcaggc tattttcagg tggtgtttaa agcgttagag cttatagacg aaaagtttaa
     7381 aggcaaagag taccatttgt ctatgggcat ggtgcaatta gtaggaaaga aaatgtcttc
     7441 tcgcacgggc gttttagtta ccgtagacgg cctgctcgac gacgtaaaag aacttttggc
     7501 gcaacttata accagcgagg ggttcacgcc gcaagagaaa gaagaagtaa aagaaacagg
     7561 caccgtggct gcggtgaaat actcggtgct caaaacagat accaaagcaa acgtattgtt
     7621 tgatttggcc cgttctgttg ctttggacgg caactccggg ccttacttgt tgtacacata
     7681 cgctcggtgt aagagtattt tgcgcaaaga aaaagcgagc aataaacaaa taaaaattag
     7741 caatgtagca aaagaagagt tggatatttt gcgtttgatg tatcgcttcc ccgaagtggt
     7801 gcaggaagcc gcagaaaagt atgcgccaaa cttagtgtgc gcttttgcct ttgatctgtc
     7861 gcaaaagtat aacgcgtttt acaacgcgca ctcggtgctg caggcagata cgccagaaca
     7921 aaaagaattc cgcttgcagc tttgcgcagc ctgcgcccag cttttgcaaa acagcctgca
     7981 tcttttaggc attcaaactt tggagaagat gtaaagaaat tgaaaattcc tctttaaagt
     8041 gatagggtaa tacattatgg aggcggactt tccgaaactg gaggaacaca tactcaaacg
     8101 ctggaaagag caaaaagcgt ttgaaaaatc tcttgagagg cgaaagaaag cgcctcgttt
     8161 cgtgttttac gacggccctc caacggcaaa tgcacctccg ggatttcacc acttgctgtc
     8221 tcgttccatg aaagacgtgg tactgcgcta ccggacaatg cgcggatttt ttgttgcaag
     8281 aaaggccggc tgggatacgc acgggttgcc cgtagaattg caaattgaaa aaaagctcgg
     8341 gttgcagtcg aaaaaagaaa tagaaaaata cggcatagca aagtttaaca aggcgtgtcg
     8401 ggactcggtg tgggaattta tccaagactg gcgcaatctt agcgagcgta cgggtttttg
     8461 gattgacatg gacaatccgt acattaccta taatacctcg tatatcgaaa gcctgtggtt
     8521 tgtgatacgg cagtttgcaa aaaaagggtt gttgtacaaa gactataaag tggtgcccta
     8581 ctgcccaaga tgcggaacgc ccgaatcctc tcatgaattg gcgcagggct acaaaaccgt
     8641 aaaagaccgt tccgtgtatg caaagtttaa aattaaggga gaaccaagta cctatttttt
     8701 ggcctggacc acaacgccgt ggacgctgcc gggaaacgtt gctttggcag taggagaaaa
     8761 aattacctac gccttcgtag aacaaaaagg cgagacgtat atccttgcaa aaagtctttt
     8821 gaacaagctc gagggcgagt acaaagttat caaagaagtt ttgggaaaag atttggaggg
     8881 gctagaatac gagccgttgt ttgattcgtt gcaaaaccag ccagagaaaa agcactatgt
     8941 ggcgcaggcg agctttgttt ccaccgacga gggaaccggc attgtgcata ccgccgtaat
     9001 gtacggagaa gacgactacc agctgggcaa aaagctgagc cttcccaaaa agcacacggt
     9061 aaacgaagaa ggcaggttta acgaactggt gccccaatgg aaagggcttt ttgtaaaaga
     9121 cgccgaaccc ttgattatcg aagatctcaa agagcgcaac cttttataca gagaagagtt
     9181 gtacgagcac gagtatcctt tttgctggcg gtgcggcacg catttgctgt attatgccaa
     9241 agacagctgg tttataaaca tgcagaaggt aaaaaaggac ctcttggcaa ataacgagag
     9301 tattaactgg attcctgcgc atttgaagaa tggaagaatg ggcgagtggc tgcgcgaagt
     9361 aaaagactgg gcattctccc gcgaacgcta ttggggtacc cctttgcccg tatgggtttg
     9421 cgattcgtgc ggccatcagg acgtagttgg ttctatacag gatctgcaag agcaaacttc
     9481 agtgggcaat acgtattacc ttttgcggca cggcgattct gcaaggcaaa aactgcagat
     9541 tgcttctgca tggccggaaa aaaagcctct tccccttact gccgacggca taaaagaagc
     9601 cggcaaggca gccaaacagc taaaggcaaa aaagatagac atgatttttt cttctgattt
     9661 gttgcgcacc agagaaacag cagaaatcgt tgcaaaagaa ataggccggg aggtgcacta
     9721 cgacgaaagg ctgcgcgaaa actccaacgg catatttaac ggaaaatcca ttgaaaaagt
     9781 aggggccttt taccgccaga aaggcgagac tatggcagag cattatatgc gcaggtttca
     9841 tatggcgccg cccgagggcg aaagctggac tgacgtgcaa aaaagaacac tcgactttat
     9901 ctgcgaaatg gaaaaaaagc accagggtaa aaacattctt gtggtgggcc acagtttgcc
     9961 tttggcgttg ttcgaaggtt tgctggcagg gtggagcaag caagaaatag tagagcactt
    10021 tgcaaaaaac aaagctccca agttagcaag tacgggcgag tggcgaaaag tagcaggttc
    10081 caggttgccc cataacgacg aaatggacgt tgatttgcac cggccgtaca tcgacgcaat
    10141 cgattttgcc tgcaaagaat gttcaaaagg tactaggaaa cgcgtaaagg atgtggtaga
    10201 tgtttggttt gattctggag caatgccatt cgcacaagat cattggccct ttgagcaaaa
    10261 caaaaaatta accccgccaa agttttatcc ctcagattat attgtagaag caatcgatca
    10321 aacgcgcggc tggttctata ccttgcttgc tacctctact cttttgggat tggggagttc
    10381 gtttaaaaat gtaatttcca tggggcatat tctcgacgaa aaaggagaga aaatgtccaa
    10441 atccaagggc aatatcgtga acccctggga gatgattgaa aaatacggcg tagataccct
    10501 aaggtggtat ttttacacct tcaaccagcc ttgggattcc aaagcctttg tggaacgcga
    10561 tttgcagcaa accatgcgaa agtttatgct cacactttgg aattcgttgg tgttttacca
    10621 aacatacgcg acaaagccga ccaaagctgc ctcggcaaac gttctcgaca aatggattat
    10681 ttcgagactg catagtacga gcagggaagc agcaaagcgc tttgacgagt acgacattac
    10741 gggaaccgcc cggctgctgg aaagttttgt tatagacgat ctttcgcttt ggtatattag
    10801 gcgttcgcgg cagcgtttcc aaaaccccaa aagcaaaaaa gaactgcaag atgcaagcag
    10861 cactctttac ctcgtgcttt tagaaataag caaactgttg gcgccgtttg ttccgttttt
    10921 ggcagagcat atatataatg aactggatgc cagaaagcaa agcgtgcatt ggcaagatta
    10981 tccaaaagca gacgcaaaaa agataaacgc gaaattagaa aaggaaatgc aggcggccag
    11041 agatatggta accaaagctt tggcagagcg cgcaaaagca ggaataaaag tacgccaacc
    11101 cctcgccgcg ctcaaatttt caatgtccaa ttttcaattt tcaaaagacg tgactgactt
    11161 gattagagat gaagtgaacg tcaaagaagt gatgccagtg aaaaacctga aaacagaagt
    11221 agaactcgac acaaatatta caccagcctt gcagcgagag ggaacggtta gagaacttct
    11281 gcggcatatt caggaaatgc gaaagcaggc aggctacagg ccccaagacc gcgtgcgtct
    11341 gcgctatgcc ggagaagaac agctcaaaga aattttgcga gagaacaaac aaatggttat
    11401 gaaaacggtg gggctcaaag aaatgctgga aggcgaccgc cccaagcggg tattcgacgt
    11461 tgagcaggaa ttcttgctcg ataaccaaac cctctggctc ggcatccgaa agctgtgaat
    11521 agctattgac aggaagtaac ctataagtta cactatgaga aaggagattt tatacgacaa
    11581 gaatgctttg aaggagttac gcgagtttag agaagaagtt caggaagagt ttgccgcgtg
    11641 cattaaaact ctaaaacagg aaggacagct tgaatatcca gaagcaagaa aagtagcaag
    11701 aaatctattt gaaattcgag ttgtacatga aggtgcgtac cgagcgtttt atgcgtatgt
    11761 aatgaaagag tctattgtaa ttcttcattt ctttcaaaag aaaacgcaga agattccatt
    11821 aaaaaactta aagctagcac agcaacggct gaaacaatat gagtaaaaaa tacccaagaa
    11881 aaatacagca ggccattgaa agaggcgatc tcattcctta tgaggaggtt tggcgtagct
    11941 attctaaaaa gcagcgtgaa gcgattgaag agagggctcg atacttgaaa gcagccatgg
    12001 aacttcggaa agtgcgcaaa caaatgcacc tttcccagga agagcttgcc aaaaagatgt
    12061 cggtaaaacg cgagtttatc tctcgcattg aaagcggccg tcaaaatgta accctcgaca
    12121 ccctctatcg catcgccgaa gtaaccggca aagaattccg tttaagcttt agataacccc
    12181 cccaaagttt gacttttcct cggtatcatg ttatcgtatt gccagcactc gatggcagaa
    12241 aaattcccga aatttaaacc agcaatgaga ggagaaatcg ttatgggtac tgaagtcgca
    12301 accgatctat actacgatct tgacgggcag ttgcttacga taaaggagca gcttcgcaag
    12361 aaaggaggtt accgctttta tccactacag ctggcagacc atctttatgc ggcaatcgag
    12421 ggccgcttta tgtctcgaga cggtaggcca ttactagtag agcccgtttt ctcgccgatt
    12481 cctggcgacg gcgaggaatt cgagctcatc ctcagggaca aagtcgaccc aatggaaatg
    12541 gtcagaaatt cagggtgcga ccccgacgac tggaagttca ggggcgagca aatcattgtg
    12601 ccttcaatcc accatttcaa actggtccag gtaggatact gcagggactt tacggaggtt
    12661 caggataaac ttacttcgca cggaaacccc gcaaatggtt ggtggagaga ggtttttcag
    12721 aaaacctacc caagaagtga cggaaaaggt cccatcggtt ttctcgacca ttcttggata
    12781 gatccggctg gacgcacacc ctttcctggg ttggtttgtt ctaggggaca tggtcgccat
    12841 tactggactc ctcgtttctt ctacgttcaa cgcctccgtt cctataactg gcgttggctc
    12901 gttgaagtcg aagagtagag tgtcggcaga aacatagata catcagtccc cgtagcggtt
    12961 acgcaaagcg gggacttttc ttttacaaga ataagtatga tataaaaagt tatggcgagg
    13021 tactatcgca cgcagggaat agtgctagaa aaagaagacc ggggcgaggc agaccgcgtg
    13081 tttaccgttt ttaccaaaga tttcggcaag ctgcgcctcc gggcggtttc ggaacgcaaa
    13141 attacttcga aactgcgcgg cggcttggaa cttttttacc ttgcagaaat cgagtttgtg
    13201 cagggcaaaa cgcaaaaaac cgttaccgat gcctgggcaa tagaccaaca tatattgttg
    13261 cggcgagatg taaaccgcct tcgggtaatg caccggttgg ccgaaattgt tgacgacttg
    13321 gtaaaagggc aagaggaaga ccagaggctg tggagcctgc tttcagaaac agttgccttt
    13381 ctgaatcgtt ttgatatgaa ggcccgagag gtgcgtttgg tttcctatta ctttctctgg
    13441 aacttgtttg caacactggg gtacagaccc tcgcttggcc ctatagccga gaggaacgaa
    13501 aaggttgcag cagttatccg aacctttctt cagggcgacc ttgcggcctt gcaagatctc
    13561 tcgaaagctg gtatacaaga agaccttttg caaaagattt gccaggagca tctttcgaag
    13621 gtgcaagaaa gttgatacaa tatatacaat gcgtgtaaaa tttgtttttg cctctttgtt
    13681 tgccaccgta gccttagccg cgcttggttt ttggttttgg cagcaaaaca cattttctac
    13741 aggaagcctg aaactcgaaa ttttagctcc cgaagaaatt accatgggcc aggagattac
    13801 ctatgtggtg cggtggaaaa acaacggcga aaccgaactc gaaaatgtag agctggtatt
    13861 tgagttcccc gagggtacgg tgcttgccca gggaggagaa ttgcggatta tcaaacagct
    13921 ggatgatgta aattccggcc aggaaggatc ggagcgcttt gtgggaagga ttttcggaaa
    13981 ggaaaatgaa ctgaaagaag caaaggcgtt tcttacctat tcgcctaaaa acttaagcgc
    14041 ctcttttcaa aacgaaacca agacgagctc ggagatttcg tttgtgccca ttaactttga
    14101 actggacatt ccttcgcgca ttgagtcggg gcagcagttt accgttacgc tgaactactt
    14161 ttccaattct gattaccctc tgtccgactt gcgtatccaa cttgagtatc ctgcaggatt
    14221 cgagtttcgg ggagcgaacc ccgcgcccat tggcgaaaac gagtgggaca tcggcctgtt
    14281 aaacaaggca gaaggcaggc gtattaccat taccggagcc atacaaggcg acgtgcagca
    14341 ggtaaaaaac tttaaagcca cattcggaag ctggaaagaa ggaaggttta ccgtaatgaa
    14401 agaggtaacc aaggctatgg aaattaccaa gccccgtatt tttattacgc aggtggtaaa
    14461 ttcttcgccc agttacgtag cttctctggg agacacgctg cattacgaag tattctttcg
    14521 gaatccgggc gaccgcattt tggaaaacct ctttttaatc gttacgctcg aaggccgcgc
    14581 ttttgacttg cagagcgtaa aagcgcaaaa cggaaggttt caaagcggag acaattccat
    14641 tgtatgggaa gcaaaagacg ttcccaagct ccggtttttg ggcacgggag aggagggcaa
    14701 ggtggagttt tggataaacg taaaagacga aattgacacc gcaagcccac aggaaacgga
    14761 gctggtgctg aaagaccgcg tgttgttgtc tgaagccacc gaagagttca cggtaaagtt
    14821 gaacacgggt cttaccattg aacaaaaagg attttaccag gacgaggtgt ttggcaactc
    14881 gggccccatt cctccaaaag tgggcgagaa aacaacgtat actattactt ggcaggtaaa
    14941 gaatcgctac aacgatatac gaaatgcgag agtaaaggct attcttcccg ctagagttga
    15001 actcacagga gaaattttcc cggaggacgc aattcttacc tttgattcgg ggtctcgcga
    15061 agtggtgtgg gaagtgggcg acgtgccagc cggatcagga tattttgaaa ccgtgccggc
    15121 gcagtcggta gcatttcagg ttgcctttac gcccacgtca gtgcaccgcg gagatgtggt
    15181 agatcttatc cgggaagtgc gtgttaccgg agaagacgtg tttaccgatc aggtggtttc
    15241 ttcttcagat ggggctatag ataccacatt gccagacgac gactccgtgg acgaagacga
    15301 cggcgaggta agaggataaa gaaaggcccg ttctcgcggg gcttttcttt tgggcttaaa
    15361 tatgcttaca tagccatatg gaagatctca tgaagaaaat cgtttccctg gccaagcggc
    15421 ggggttttgt attccagtct tctgaactgt atgggggcgt ggaagcgttg tgggactttg
    15481 ggcctctcgg ggctgccctt aaagaaaaca tcaaaaaagc ctggatgcgg cgctttgtgc
    15541 agcaaatgcc gaatgtggtg gccattcaag gatctattat tatgcatccg gacgtatgga
    15601 aggcttccgg gcatttggaa aactttaccg atccactcgt cgaatgtaaa aagtgcaatg
    15661 ccaggcttcg ccaagaccat atcgaagacg gcaagtgccc taactgcggc agcaaagaat
    15721 tttcagaggc cagaaacttt aacttaatgt tcaagacatt tttggggccc ctggaagatg
    15781 aaggccatac tgcgtatttg cgcccggaaa ccgcacaaag catgtttacc aattttaaat
    15841 tggtgcaaga aagcatgcgt cagaaaattc cgtttgggat tgcgcaaacc ggaaaggctt
    15901 ttcgcaacga aattaccacg gggaacttta ttttccgctc gcgcgaattc gagcaaatgg
    15961 aaattgagtt tttcgtaaaa ccggggcaag acgacaaatg gtttgatttt tggatagacg
    16021 cctgggaaaa gttttatttg gatttaggaa tcaaaaaaga aaacttgcga agatatgagc
    16081 accccaaaga aaagctgtcg cattattcca agcgtacggt agacttgga
//
LOCUS       DASRUP010000060         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_238080, whole genome shotgun sequence.
ACCESSION   DASRUP010000060 DASRUP010000000
VERSION     DASRUP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238080"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="VFE94_02420"
     CDS             complement(<1..1965)
                     /locus_tag="VFE94_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49985.1"
                     /translation="MIIKFIPVIKLIKVITIITVLLLGNFSAAQAAVMSSSTYQIEID
                     SLNMGGLRATSSSYVLEQTGGESATGILSGSSNSLYAGFQLPVAAAPSPTPSPSPAGG
                     PAFIPFVIIDVVVESVTTDTVTITFRTNKTATTETNFGTTDAYGQAVVNPAPQSSHTV
                     TLPNLQPGTSYYFEITAKTAGGEETTTTVYIVKTLPPSVPPEKEAPLNVENFVASPGD
                     REIELTWKNPLDTDFAGVKIMRSEEFYPTGSSEGFVIYEGSAESFLDTGLENGTRYYY
                     TAFAYDTDSNFSSGAIASAIPQPAGVPPVIPPPGPPLVPPELIPETLKDLSFLDFDFI
                     QQGSKLPVIGGRVEVKPDQPLTVSIDYDKLPEVLKTILVTMRDEEGKTFSFLLRVDEE
                     KQRYSATLKPPVAGLYSLTFTVVDYQNQGLQKVEGELKVKEVAGAVVAPPSEGFGRFL
                     PELAVFFLLALLLLMLWFGAKKYIGSRSRTAARYIGCIVFALFLLGFVFGGTAEAAFN
                     PELNYQGKLTDTSNDAVSDGDYNFKFQLCTDSACVASVWTETHETTNKITVTSGVFSV
                     LLGDVTSIASVDFNQELWLEVQVGGTGSSPSYETLTPRKKLGAVAAAFEADKLDGLDS
                     GSFVRSDAADTVSGLLTITADPTGSGVGAGS"
     gene            complement(1962..>2596)
                     /locus_tag="VFE94_02425"
     CDS             complement(1962..>2596)
                     /locus_tag="VFE94_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX49986.1"
                     /translation="SNPPTNLPIFVSEIDAAEDPTPEEMEQRKTFQRKAGFAIAFAMV
                     LLAANFGMFQGRQEVARVAEYVSPAVIQATDKVEYELIGVGFETGEQIEATLNSFARQ
                     FYAGGVGEIARGYFGWLKNLLFQDRFVVNDKSTPPPPAAPGSPQNDTGLVVVPSGQDT
                     AQLLSQVKQSFSDEVKVTPYDEESGIITPVFRHRTGEDYLYILVPLQESE"
BASE COUNT          664 a          681 c          606 g          645 t
ORIGIN      
        1 agaacctgcc ccgacgccgc tacccgtagg atctgccgta atggtaagca aaccagacac
       61 ggtgtctgct gcatccgacc tcacaaaaga tccagaatcc aaaccatcga gcttgtctgc
      121 ctcgaaagct gctgcaaccg cgcccagctt tttgcgggga gttaaggttt cataacttgg
      181 gctgctgccc gttcccccca cctgcacttc gagccacagt tcctggttaa aatctaccga
      241 agctatgctc gtaacgtccc caagcaacac gctaaaaact ccgctggtta ccgtaatttt
      301 attcgtagtt tcgtgggttt ccgtccatac agaagctacg caggcagagt cggtgcacaa
      361 ctggaattta aaattatagt cgccgtcgct taccgcatca tttgaagtat cggtaagttt
      421 tccctggtaa ttcaattcgg ggttaaaagc agcttcggct gttcctccaa atacaaaacc
      481 aagaagaaaa agggcaaaaa caatgcatcc gatatacctg gcggcagttc ttgagcgcga
      541 cccgatatac tttttcgccc caaaccatag cataagcaaa agcagcgcta gcagaaaaaa
      601 taccgccaat tcgggcaaaa acctgccaaa gccctcgctt ggcggagcaa cgacagctcc
      661 cgccacttcc tttactttca actcgccctc cactttttgc aagccctggt tttgataatc
      721 caccaccgta aaagtaagcg agtacaaacc ggcaacaggg ggctttaacg tggctgaata
      781 ccgctgcttt tcttcgtcta cccgcagcaa aaaggaaaat gtcttgcctt cctcatccct
      841 cattgttacc agaatcgttt tcaacacttc aggaagtttg tcgtagtcta tggaaaccgt
      901 aagaggctgg tctggcttaa cctcaactct cccgcctata accggaagct tgctcccctg
      961 ctggatgaaa tcgaaatcca aaaacgacag atcttttaag gtttcaggga taagttccgg
     1021 gggcaccaaa ggcggcccgg gcggcggaat tacgggaggg acaccagctg gttgaggtat
     1081 ggcagaagca attgcgcccg aggaaaaatt cgagtctgtg tcgtacgcaa atgcggtgta
     1141 gtaataccgc gttccgttct cgaggccggt atcgagaaac gattctgccg aaccttcata
     1201 gataacaaag ccttcggaag aaccggtagg ataaaattct tctgaacgca taatctttac
     1261 cccggcaaaa tcagtgtcta atgggttctt ccacgtgagc tcgatttcgc gatcgcccgg
     1321 acttgcaaca aaattctcca cgttcaaagg agcctctttt tctggaggta cgctcggagg
     1381 caatgttttc actatataca cggtggtcgt tgtttcctcg ccccctgcgg tcttggcagt
     1441 aatctcaaag taatacgaag tccctggctg gagattcggg agagttacgg tatggctgct
     1501 ttgcggggcc gggtttacta cagcctggcc atacgcatct gtagtcccaa agttggtttc
     1561 tgttgtggcg gttttgtttg tgcggaatgt aatggtaacc gtgtctgtag ttacactttc
     1621 aacaacaaca tcaataatca caaaagggat aaaggccggg cctcctgcag ggctgggcga
     1681 tggggtcggg cttggcgcag cagcaacagg aagctgaaat cccgcgtata agctatttga
     1741 gctgccagaa agtattccgg tggcagattc tcctccggtc tgttccaaca cgtagctcga
     1801 cgacgttgcg cgcaaccctc ccatatttaa agaatcaatc tcaatctggt acgtgcttga
     1861 cgacatcacc gcagcttgcg ccgccgaaaa attccccaga agaagaacgg ttataatggt
     1921 tataactttt ataagtttta taactggtat aaattttata atcattctga ttcctgaagc
     1981 ggcactaaaa tatacaaata atcctcgccg gttcgatggc gaaacaccgg ggtaataatg
     2041 cccgattcct cgtcgtaagg cgtaaccttt acctcgtcgg aaaaggactg cttaacctgg
     2101 ctaagcaact gggcagtatc ttgcccagaa ggcactacca ccaaaccagt atcattctgg
     2161 ggcgagccag gtgcggctgg cgggggtggc gtgcttttat cgttcacaac aaaacggtct
     2221 tggaacaata aattcttgag ccaaccaaaa taccctctgg caatttcgcc aacgcctccc
     2281 gcatagaatt gtctggcgaa actgttcagg gttgcttcaa tttgctcgcc ggtttcaaaa
     2341 ccaactccga tgagttcgta ttctacttta tcggttgcct ggatcaccgc aggtgaaacg
     2401 tactctgcga cgcgcgccac ttcctgcctt ccctgaaaca ttccaaagtt tgccgcaagc
     2461 aacaccatgg caaatgcaat tgcaaagcct gcttttcttt gaaaagtttt tctctgttcc
     2521 atttcctctg gcgtagggtc ttccgccgcg tcgatctcgg aaacaaaaat cggaaggttt
     2581 gtcggcggat tgcttg
//
LOCUS       DASRUP010000061         6475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1129617, whole genome shotgun sequence.
ACCESSION   DASRUP010000061 DASRUP010000000
VERSION     DASRUP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129617"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFE94_02430"
     CDS             complement(<1..401)
                     /locus_tag="VFE94_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HZX49987.1"
                     /translation="MAKQILYSEDARKKLKAGVDKVANAVKVTLGPKGRNVVLDEGFG
                     SPTITNDGVTIAKKIEVEDKIENIGAEIIKEVASKANDVAGDGTTTATVLAQAVIGEG
                     MKNVTAGANPLALRRGLEKATGNIVESLKQMS"
     gene            complement(418..708)
                     /locus_tag="VFE94_02435"
     CDS             complement(418..708)
                     /locus_tag="VFE94_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZX49988.1"
                     /translation="MQIKPLADHVVIEPLKQEEKTASGIILPETAEKERPEQGKVIAV
                     GPGRKTEDGKVVPLDIKVGDVVLFTKYGPNEVKVGDKEYLIAKEDDILGILE"
     gene            complement(816..3611)
                     /gene="uvrA"
                     /locus_tag="VFE94_02440"
     CDS             complement(816..3611)
                     /gene="uvrA"
                     /locus_tag="VFE94_02440"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZX49989.1"
                     /translation="MPKKDHSQVIKIRGARVHNLKNIDVDIPKNKLVVITGLSGSGKS
                     SLAFDTLYAEGQRRYVESLSAYARQFLGVMDKPDVDSIEGISPAIAIDQRKGSHNPRS
                     TVGTITEIYDYLRLLFARVGIPHCPNCGKEVKRQSVSQIAEQVLKLPKGAEITILGPL
                     VRGRKGEHRGTLEEVQRAGFIRVRVNGVVHRIEEALQKELDRKRKHTIEVVVDRLVGE
                     EHMDKTRLVDSLETALKLGKGIVMVNDMIFSEHFACEECGISLPAIEPRLFSFNSPFG
                     ACPSCQGLGEKLEVDPSLVIPNPNLSIAEGAIFPWAKASHKIGRQGLFWWMLEELANE
                     YGFSLRTPTKELAKKDLDMILYGAGEFEGVIPWLERRYHETDSDYAREEIEQYMIEKQ
                     CEVCKGKRLKPEVLAVTVAEKSIDSVVQASIEQNKEFFQSLHKNLKESEKKIAGPIAK
                     EILNRLQFLIDVGLEYLTLARKAGTLSGGEEQRIRLATQIGSKLSGVLYILDEPSIGL
                     HSRDQHRLIETLKQLRDVGNSVIVVEHDPQTIRESDWIVDIGPGAGKHGGKVMFEGTP
                     KAVLKSNTLTGEYLSGKKKVALRKTNGGPGRSGQSQRFLVVKGAKEHNLKNIDVKIPL
                     GKFVCVTGVSGSGKSSLVNDILARALLKKFYRAKDEPGEHKEITGVQYLDKVVLVDQS
                     PIGRTPRSNPATYTGSFAHIRDLFVKTKEARVRGYEAGRFSFNVKGGRCESCEGQGLK
                     KIEMYFLPDVYVECEECEGKRYNKEVLEILYKDKNIADALAFTVEEGLEFFKNIPPIA
                     AKLKTLNDVGLSYIELGQPAPSLSGGEAQRVKLATELAKKATGKTLYILDEPTTGLHF
                     DDIRKLLAVLTELVAKGNTVVTIEHNIDFIASADWIVDLGPEGGDKGGFIVAEGPPKE
                     IAKNRKSYTGQYLKELV"
     gene            4052..5335
                     /locus_tag="VFE94_02445"
     CDS             4052..5335
                     /locus_tag="VFE94_02445"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZX49990.1"
                     /translation="MKFQYVKKENIAEIPKTPGVYSFWDKNSLLYIGKAGNLRDRVKT
                     HFAQPSYRDNLFMDQVGKIGFMETDSEIEALLLESVLIKAKNPKYNVMWRDDKSYFFV
                     AITKEKLPRVFLAHQTSREKTPNTKYQILNTSYLGPFTDGKAVKRTLRSLRRVFPYYA
                     AKRHSLRQCQYCNLGLCPGPEPDLVKYKKDIKNLVAVLQGKKQSVLKTLQKEMAAASK
                     AQQYERAKELRDQLRDLEVIFSHARIFRPTDADASVGVNWPAIEQYLQKILGTKKRIS
                     RAEAYDISNTQGKEATSSMPVFVNGVPAKSQYRKFKIRLGNTPNDFAMLAETLSRRLC
                     HAEWPYPDLMIIDGGKGQLSSALKAINSQQPTILVAAIAKKNNELFLPGKSEPLLLRD
                     MPQPVSNFILHMRDEAHRFAITYHRKLRKVDLLGK"
     gene            5352..5705
                     /locus_tag="VFE94_02450"
     CDS             5352..5705
                     /locus_tag="VFE94_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZX49991.1"
                     /translation="MIQMLLLHILAGIGGLWIAVRIVDGVEFTGPAYLLLIIGLVLGI
                     LNAIVKPVLDLITLPIRILTLGLSSILINLGLVWALQLVFPELEFEGFLPLLWTTLII
                     VGITIPLSVLSRGKI"
     gene            5702..5929
                     /gene="secG"
                     /locus_tag="VFE94_02455"
     CDS             5702..5929
                     /gene="secG"
                     /locus_tag="VFE94_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZX49992.1"
                     /translation="MTFDFLISLVQIGVAIFLVVFILLQQRGTALGSAFGGEGGSFYG
                     ARRGLQQKLYWGTIVLGALFIALSLARLILI"
     gene            5931..>6475
                     /locus_tag="VFE94_02460"
     CDS             5931..>6475
                     /locus_tag="VFE94_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX49993.1"
                     /translation="MSSQTNPLQDNIPSVTPQGSNRRWPTFAQWIQLPRLLSQKERVA
                     LVILVCVFFGSSFSLARGWYLEHTVVVPARGGSIVEGLIGSPRFLNPVYADANDVDRD
                     LLQLLFSGLYAYNSQGELVADLAQGNPEITEGGKVVTVSLKEDVKWHDGEEFSADDVL
                     FTIQTLKDPAYKSPVRANWIGV"
BASE COUNT         1570 a         1631 c         1477 g         1797 t
ORIGIN      
        1 gacatttgtt ttaaagattc cacgatgttt ccggtcgcct tttccaagcc ccggcgcaaa
       61 gccaaaggat tggcgccagc ggttacgttt ttcatgcctt ccccgattac cgcctgggca
      121 agaaccgtgg ctgtggtagt cccgtcgccc gcaacatcat ttgctttcga cgccacctcc
      181 ttgataattt cagctccaat gttttctatt ttgtcttcta cttcaatttt tttggcaatg
      241 gttacgccgt cgttggtaat agttggagaa ccaaaacctt cgtcgagcac gacgttgcgc
      301 cctttggggc cgagtgttac ttttacggca tttgccactt tatcgacacc tgcctttaat
      361 ttcttgcgag cgtcctcgct gtagagaatt tgctttgcca tagtgagttt tatcgaacta
      421 ttctaaaatt ccgaggatat catcctcttt agctatcagg tattccttat ctccgacttt
      481 tacttcgttg gggccgtatt tggtaaacaa aaccacgtcg cccactttga tatccaaagg
      541 cacaactttg ccgtcttcgg tttttctgcc agggcccacg gcaataactt taccctgttc
      601 ggggcgttct ttttctgcgg tttctggcaa gataatgcca gacgctgttt tttcctcttg
      661 cttgagaggt tcgatcacca cgtgatcagc taagggtttt atctgcattg ttgattgtta
      721 attattgatt gctgattgtt tagcactcga ccaagaagac tgctaatttc gtacccgtat
      781 tatagaaaat ccccttcaaa aagtcaagaa ccccctcaaa ccagctcttt taaatactgg
      841 ccggtgtagg actttcgatt ctttgcgatt tctttgggcg ggccttcggc cacgataaag
      901 ccgcctttgt cgccaccttc gggaccaagg tcgactatcc agtcggcaga agcaataaag
      961 tcgatattgt gctcaatggt taccacggtg ttcccttttg ccaccaattc ggttaacacg
     1021 gccaaaagtt tgcggatgtc gtcgaaatgc aggccggtgg taggctcgtc taaaatatac
     1081 agggttttgc ccgtggcttt tttggcaagc tcggttgcca gttttacgcg ctgcgcctcg
     1141 ccgcccgaaa gcgaaggggc tggctggccc agttcaatat agcttaagcc tacgtcgttt
     1201 aacgttttga gtttggcagc aatgggcgga atgtttttga aaaactccag cccctcctct
     1261 accgtaaagg ctaaagcgtc ggcaatattt ttatctttat acaaaatctc gagcacctct
     1321 ttgttatagc gctttccttc gcactcttcg cattccacat agacgtcggg caaaaagtac
     1381 atttcaattt tttttaaccc ctggccttcg cacgactcgc accttccgcc ttttacgtta
     1441 aaggaaaacc tgcctgcttc gtacccccga acgcgggcct cttttgtttt tacgaataaa
     1501 tcccgaatat gcgcaaagga tcccgtatac gtagcgggat tggaacgggg cgtgcggcca
     1561 atgggcgact gatccacaag taccactttg tctaagtatt gcacgccggt aatttctttg
     1621 tgttctccgg gttcgtcttt tgcgcggtaa aactttttga gcagggctct ggccaagatg
     1681 tcatttacca aagaactttt gcccgatccc gacacgcccg taacgcacac aaattttccc
     1741 aaaggaattt ttacgtcgat attttttaaa ttgtgttctt tggcgccctt taccaccaaa
     1801 aagcgttggg actgccctga ccggccaggc ccaccgttcg ttttgcgcaa cgccaccttt
     1861 ttctttcccg aaaggtactc gcccgttaac gtattcgatt tgagtactgc cttgggcgtt
     1921 ccttcgaaca ttactttgcc cccgtgttta ccggctcccg gaccaatgtc gacaatccag
     1981 tcggattccc gaatcgtttg cgggtcgtgc tccaccacaa taacgctatt gcccacgtcg
     2041 cgcagctgct ttaaggtttc gattaagcgg tgctgatcgc gcgagtgcag cccaatcgaa
     2101 ggctcgtcta aaatatagag caccccggaa agcttggatc caatttgcgt ggctaagcgt
     2161 atgcgctgct cttctccccc ggaaagcgtg cctgcttttc ttgcaagggt taaatattcc
     2221 aagccaacgt cgattaaaaa ttgcaggcga tttaaaattt cttttgcaat ggggcctgcg
     2281 atttttttct ccgactcctt gagattttta tgcaggcttt ggaaaaattc tttattttgc
     2341 tcaatgcttg cctgcactac gctgtcgatt gatttttctg ccacggttac cgcaagcact
     2401 tcgggcttta gcctcttgcc tttgcacact tcgcactgct tttctatcat gtactgctca
     2461 atttcctctc tggcataatc tgaatcggtt tcgtggtaac ggcgttccag ccaaggaatc
     2521 acgccctcga attcacccgc accatacaaa atcatgtcga ggtctttttt agccaactct
     2581 tttgtgggcg tgcgcaaaga aaacccgtat tcgtttgcca gctcttctag catccaccaa
     2641 aaaagcccct gccttccaat tttgtgggaa gcttttgccc acgggaatat agccccctct
     2701 gctatggaaa ggttgggatt cggaattacc aaactgggat ctacctctaa cttttcgccc
     2761 aacccctggc acgaagggca cgcgccaaac ggcgagttaa acgaaaacag ccgaggctca
     2821 atagcgggca agcttatacc gcactcctcg cacgcaaagt gttcagaaaa aatcatgtcg
     2881 tttaccatga caatgccttt gccaagcttt agcgcggttt ccaaagaatc caccaggcga
     2941 gtcttgtcca tgtgttcctc gcccaccaaa cggtctacca ctacttcgat ggtatgcttt
     3001 cgcttgcggt cgagctcttt ttgcaaagct tcctctatgc ggtggactac cccgtttacc
     3061 cgaacacgaa tgaagcccgc acgctgcact tcttccaacg ttccccgatg ctctcccttt
     3121 cttcctcgca ccaaagggcc aagaatggta atttctgctc ctttcggaag tttgagcact
     3181 tgttctgcaa tttggcttac gctttggcgt tttacttctt taccgcaatt ggggcagtgc
     3241 ggaatgccaa cgcgcgcgaa caaaagccta agatagtcgt aaatctcggt aatggttcct
     3301 accgtggagc gcgggttgtg cgatcctttg cgctggtcta tggcaatggc agggctaatg
     3361 ccctcaatgc tatccacgtc gggcttgtcc attacgccca aaaattgccg agcataagcc
     3421 gacaaacttt ccacgtatcg gcgctggcct tccgcgtaca acgtatcgaa cgcgagcgaa
     3481 ctttttcccg agccagacaa gccggtaata accaccagct tgttctttgg aatgtccaca
     3541 tcaatatttt ttaagttgtg caccctggcg cctcgaattt tgataacttg agaatggtct
     3601 tttttcggca tatcagtcta gaattataca ccaaaagtca accccgtaca agttcgcttt
     3661 gtaccggggt acataatact caatattgca atttgccaga ttttatgttt cattaagaaa
     3721 aactacaaca gcgaggcgta gcatgttttt gcgaatgtgg ttgtatacag caaccatttt
     3781 tatcctatct gttctgtcct taaacattct gaaagccatt cccgctattc gtgaaacacc
     3841 aggagcggtt ccaggagtta cgatattgat agttacgtgg agtgttcttg ttggcgttac
     3901 attagatctt cgccaaaacc taaaaagaga gaccgaaagg cgctagagaa aaaacccggg
     3961 aggagaatct ctcccgggct ttatttttaa ggttcaacac tattatggta cttatatgtc
     4021 gctgaaaata tggatttgct ataatgggct tatgaaattt cagtatgtga aaaaagagaa
     4081 cattgcggaa attccgaaaa caccgggggt gtattcgttt tgggataaaa acagcctgct
     4141 ctatataggt aaggccggga atttgcgcga tagggtaaaa acgcattttg cccagcccag
     4201 ctaccgcgat aatttgttta tggaccaggt aggcaaaatt ggctttatgg aaaccgactc
     4261 ggaaattgag gcgttgctgc tggaatccgt tcttataaaa gcaaaaaatc caaagtacaa
     4321 cgtaatgtgg cgcgacgata aaagttattt tttcgtggcc ataaccaaag aaaaactccc
     4381 cagagttttt ttagctcacc aaacctcaag agaaaaaacc ccaaatacta aataccaaat
     4441 actaaatact agttatcttg gcccgtttac tgatggaaag gcggtaaaac gaacattgcg
     4501 gagcttgcga cgggtatttc catactacgc ggcgaaaagg cacagcctgc ggcagtgcca
     4561 atactgcaac ctggggctct gccccgggcc ggaaccggat ttggtaaaat acaaaaaaga
     4621 cataaaaaat ttagtcgcgg tattgcaggg caaaaaacaa agcgtgctaa aaacactgca
     4681 aaaagaaatg gcggcagctt caaaagccca gcagtatgaa agggcaaaag aactgcgcga
     4741 ccagctgcgc gatttggaag ttattttttc gcatgccaga atatttaggc caaccgacgc
     4801 cgacgcttcg gtcggggtaa actggccggc catagaacag tatttgcaaa aaattcttgg
     4861 cacgaaaaaa cgcatttccc gcgcggaagc atacgacatt tcaaataccc agggcaaaga
     4921 agccaccagc tctatgcctg tgtttgtaaa cggcgtgccg gcgaaaagcc aataccgcaa
     4981 atttaaaatt cgcctgggca acacgcccaa cgactttgcc atgctggcgg aaaccctaag
     5041 ccgccgcctt tgccacgcag aatggccgta tcccgattta atgattatcg atggcggaaa
     5101 agggcaattg tcgtcggcgc tcaaagcgat caatagtcaa caaccgacaa ttctcgttgc
     5161 cgctatcgca aagaaaaata atgaactttt tctgccgggt aagtcggagc ctttattgct
     5221 gcgcgacatg ccccagcccg tttcaaattt tattttgcat atgcgcgacg aagcccaccg
     5281 tttcgccata acctatcata ggaaactacg aaaggttgac cttttgggca aataggggta
     5341 tattagatac aatgatccaa atgcttctct tgcatatttt ggcagggatt ggaggcctct
     5401 ggattgccgt tcgcattgtt gacggagtgg agtttacagg ccccgcctac ctcctcttga
     5461 ttatcggact ggttcttggt atactaaacg cgatcgtaaa acccgttctc gatcttatta
     5521 ctcttcccat acgaatctta acgcttgggt tgtcgagcat actcattaac ctgggcctgg
     5581 tatgggcgct tcaactggtt ttccccgaac tcgaattcga agggttcctt cctctgttat
     5641 ggacaactct tattatcgta ggaattacga ttccgctttc ggtcttaagt agaggaaaga
     5701 tatgactttc gattttctta tctcacttgt gcagatcggc gttgcgatct ttcttgtggt
     5761 gtttatactc cttcagcaac ggggaaccgc tttagggtcc gcgtttggtg gcgaaggagg
     5821 aagtttttat ggggcgagaa gagggctgca acaaaagctg tactggggaa ctattgtcct
     5881 gggcgctttg ttcattgctc tctcgcttgc ccgccttatt ctcatttagc gtgtcatctc
     5941 aaactaaccc cctccaagat aatataccct cggtaacgcc tcaaggcagc aatcggcgtt
     6001 ggcccacatt cgcccagtgg atacagcttc cacgcctgct ttcccaaaaa gaacgggtag
     6061 cgttggttat tttggtatgc gtattctttg gttcttcctt ctcccttgcc agagggtggt
     6121 acttggaaca tacggtcgtg gtgccagctc ggggaggcag tatcgtggaa gggctaatcg
     6181 gatcgcctcg ctttctcaat ccggtgtacg ctgacgcaaa cgatgtagac agagatctgc
     6241 tgcagctttt gttttcgggc ttgtacgctt acaacagcca gggcgaactg gtggccgatt
     6301 tggcgcaagg aaatcccgaa atcaccgagg gaggaaaagt ggttacggta tctctcaagg
     6361 aagacgtgaa gtggcacgat ggagaggagt tttccgccga cgacgtactc tttaccattc
     6421 agaccttaaa agaccctgcg tataaaagcc ctgtgcgcgc caactggata ggggt
//
LOCUS       DASRUP010000062         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_696329, whole genome shotgun sequence.
ACCESSION   DASRUP010000062 DASRUP010000000
VERSION     DASRUP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696329"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFE94_02465"
     CDS             complement(<1..960)
                     /locus_tag="VFE94_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HZX49994.1"
                     /translation="MTNIAINGFGRIGRLFYRQIAAKKGFEVVAINDLGNAENLAYLL
                     KYDTVYGKFEGKLPATVLQEKDPAKLPWKKLGIDIVVEATGVFDSFEKSKAHLQAGAK
                     RVVITAPADDADGELGRTVLMGVNDKDLAMCKISSNASCTTNSIASVIQVLDEAIGVS
                     KALLNTTHGYTATQSLVDSPVKGKDLRRGRAAAQNIIPSSTGAAIATTRAVKSLQGKF
                     DGIALRVPVPAGSIADITFVAKRITTVEEVNSALTKAAGSTRFKNILQVTSDPVVSAD
                     IVGQPYGAIVDLAFTRVVDGDLVKVLSWYDNEWGYASTLVKHVE"
     gene            complement(967..1830)
                     /locus_tag="VFE94_02470"
     CDS             complement(967..1830)
                     /locus_tag="VFE94_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZX49995.1"
                     /translation="MVETSQALVKKIYRPRPAESRKYDFGLMLVIGGSESYTGAPAIA
                     GLSGFRAGLDMVRVIAPKRAADIIASFSPVLAVFGVADDHLEKEHVSLLLARTMAAKE
                     AARGNVSVLIGSGLGRDEQTQEAIKEYLAQIDVPCVIDADAIHTVSEQPEILAGKPFV
                     VTPHSHEFLDLTGKEIRELPQEEKIKLVSQEALRLKTTILFKANVDIISDGKEVMLNR
                     TGTPYMTIGGTGDSLAGIVGALLARGIEPLEAAAAAAYINGRAGELASKKFKDSMIAT
                     DLIEEIPHVIA"
     gene            complement(1834..2712)
                     /locus_tag="VFE94_02475"
     CDS             complement(1834..2712)
                     /locus_tag="VFE94_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZX49996.1"
                     /translation="MAEITKAIIPLAGLATRHLPLSKVLSKELFPLADKPLLQYILEE
                     LKASGVKEVVFVVGGNRKIISDYLKRSLQLEKHLEEQKQDDLLETLREIERVCQGVSF
                     SYVPDRPLGDGHAVLQARKFVGDEPCFVVYADDIVESKTPAAMQLEQVFRTSAKPVIA
                     LFELPPDKLSSYGIVAPEKIASKLCKIKAIVEKPAKENAPSRLAMAGRSIITAEVFDY
                     LKKAKPNKRGEISLTETFGQMVKDGTIIYGYQCEGRWWECGNKEEWLKSFVHYAANHP
                     QYGREVRRFIKEEKLV"
     gene            complement(2719..4035)
                     /locus_tag="VFE94_02480"
     CDS             complement(2719..4035)
                     /locus_tag="VFE94_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZX49997.1"
                     /translation="MAKKKSLTKKPARKALRRSQGKHKSPKGKEVLPVDQVHRTRIKV
                     IGVGGGGGNIVAEIASRVHKFDFVGANTDAQALKELPKKVKTISFGHELTNGLGCGMD
                     PELGERAAKAEKERIKKLLEGNDICILIASLGGGTGSGATHVFAEASQELRNLCIGIF
                     TMPFAFEGERRKQIAEAALEKIKPFVNAYMVIPNERIFQVIDQKTSLKDSLSAVNKRL
                     TSTLEGFMDTIAMPGLVNIDFADVRSILEGRGRLAFIHSAESGGPAKAQEVVKAVLAN
                     PLCSYGIGGADRILFNIMGDRGLKMQEVAQISTAISEHNPKARIIFGISCNSRMKERI
                     KVTLFAVGCGPGEGLKAVQIKTEKEEQKKPVAVKQVRPTNTQSPAGGKKEAAIGPMGE
                     SAVRIPVRSSRVRRNALEVKKAVDQEIRELEQKEKQWDIPAFLRNK"
BASE COUNT          943 a         1190 c          908 g         1175 t
ORIGIN      
        1 ttcgacgtgc tttactaaag tggaggcgta cccccattcg ttatcgtacc acgatagcac
       61 ttttaccaag tccccgtcaa ccaccctggt aaacgccaaa tccacaatgg cgccgtaggg
      121 ctggcctaca atatctgcag aaaccacagg atctgaagtt acctgcaaaa tgtttttaaa
      181 gcgggtggaa cctgcagctt tcgtgagcgc gctgtttact tcctctactg tggtgatacg
      241 ctttgccaca aacgtaatgt cggcaatgga acctgcggga actggcacgc gaagagcaat
      301 accatcgaac ttgccctgca aactttttac cgcccgcgtg gtggcaatag cagctcccgt
      361 actcgaggga atgatattct gggcagcggc tctcccccgc cttaggtctt ttccttttac
      421 cggactgtcc accaaacttt gggtggcggt atatccgtgc gtagtgttca gcaacgcctt
      481 tgacaccccg attgcctcgt ctaacacctg aattaccgaa gctatagaat tggtggtaca
      541 agaagcgttg gagcttattt tgcacatggc caaatcctta tcgttcacgc ccatgagcac
      601 ggtacgcccc aattccccgt ctgcatcatc cgcaggcgcc gtaatcacca ccctttttgc
      661 tccagcctgc aaatgcgctt ttgacttctc gaacgaatca aatacgcccg tggcttccac
      721 tacaatgtct attcccagtt ttttccacgg aagtttggca ggatcttttt cctgcagcac
      781 ggttgcgggc agtttcccct caaactttcc atataccgtg tcgtatttaa gcaagtacgc
      841 caaattctcc gcattcccca aatcattaat tgccaccacc tcaaaaccct tttttgcggc
      901 aatctgccgg tagaacagcc gcccaatcct cccaaacccg ttaattgcaa tgtttgtcat
      961 acaattttac gcgattacgt gtggaatttc ttcgataagg tcggtggcga tcatggaatc
     1021 cttgaatttt ttggacgcca actcccctgc tcttccattt atatacgctg cggctgcggc
     1081 agcctccaaa ggctcaattc cacgcgcgag caaagcgcct acgattccgg ccaatgaatc
     1141 ccctgttccc cctatggtca tgtagggagt gccggtcctg ttgagcatta cctctttgcc
     1201 gtctgaaata atgtctacat ttgccttaaa caaaatagtg gttttcaaac gcagggcttc
     1261 ctgcgacacc agcttgattt tttcttcctg gggaagctcg cgtatttctt tgcccgtaag
     1321 atccaggaac tcatggctgt ggggagtaac cacaaagggt tttcccgcca gaatctcggg
     1381 ctgctctgaa accgtgtgaa tggcgtcggc atctatgacg caaggaacgt cgatttgtgc
     1441 taaatattct tttatagcct cttgcgtttg ctcgtcccgg cccaagccgc ttccgataag
     1501 caccgaaaca tttcccctgg ctgcttcttt tgctgccatg gtacgcgcaa gcaaaaggct
     1561 cacatgctct ttttccaggt ggtcgtcggc aactccaaaa acagccagta ccggcgaaaa
     1621 agaagctata atatctgccg cgcgtttggg agctattacc cgcaccatat cgagccctgc
     1681 gcgaaacccc gaaagcccgg caatggctgg ggctccggtg taagactccg aacccccgat
     1741 taccagcatc aaaccaaaat catactttcg ggattctgcc ggccgaggcc tatatatttt
     1801 tttgacaagc gcctgactgg tttcaaccat agtctaaacg agtttctctt cttttataaa
     1861 tcttcgcact tctcttccat attgcggatg gtttgcggca taatgcacaa aagactttag
     1921 ccactcttct ttgttgccgc attcccacca ccgcccctcg cactggtatc catatattat
     1981 ggtaccatct tttaccattt ggccaaaggt ttccgtaagc gaaatttccc ctcttttgtt
     2041 gggctttgct tttttgagat agtcaaacac ctctgcggta ataatacttc ttcccgccat
     2101 ggccaatcgg gagggcgcgt tttcttttgc cggtttttca acaatcgctt taattttgca
     2161 cagcttgctt gcaatttttt cgggagcgac aatcccgtac gaagagagtt tgtctggagg
     2221 caactcgaat agcgcaatca ccggtttggc ggaagtccgg aacacctgtt ccaattgcat
     2281 ggcagccgga gttttcgatt ccacaatgtc gtctgcgtac acaacgaaac aaggctcgtc
     2341 gcccacgaac tttctggcct gcaacaccgc atgtccgtcg cccaatggcc tgtctggcac
     2401 gtacgaaaaa gaaactccct ggcatacccg ctcgatttcc cgcagcgttt ccagcaaatc
     2461 atcctgcttt tgttcttcca aatgcttttc caactgcaaa gatcttttca aatagtctga
     2521 aatgattttt cggtttcccc ccaccacaaa taccacttct tttactcccg aagctttcag
     2581 ttcttccaat atatactgca gcaaaggctt gtccgctaga ggaaacaact ccttggaaag
     2641 aactttggag agcggaaggt gtcttgttgc caaacccgcc aaaggaataa ttgctttcgt
     2701 gatttctgcc atactatatt atttatttct caaaaacgcg ggaatatccc actgcttttc
     2761 tttttgctcc aactctcgga tttcctggtc aaccgctttt ttcacttcga gggcgttccg
     2821 ccgcaccctg gaagaccgaa ccggaatccg caccgccgat tctcccattg gcccaatcgc
     2881 agcttctttc tttccgcccg cagggctctg cgtattcgtt ggacggacct gtttgactgc
     2941 aacgggtttt ttttgttctt ccttctctgt ctttatctgg acagccttga ggccttcccc
     3001 cgggccgcag cctactgcaa acaaagtaac cttgatacgc tctttcatgc gcgagttgca
     3061 cgaaatgcca aaaataatgc gggctttggg attatgttct gaaatggcgg tcgaaatctg
     3121 cgctacttcc tgcattttta atccgcggtc gcccataata ttaaacaaaa tgcgatctgc
     3181 gcctccaatg ccgtaactgc acaaaggatt ggcaagcacc gctttcacca cttcctgggc
     3241 ttttgccggc ccgccgcttt ctgccgaatg aataaacgcc agcctgcctc tcccctccag
     3301 aatggaacgc acatcggcaa agtcaatgtt tactaaaccc ggcatggcaa tcgtatccat
     3361 aaacccttcc agggtagagg ttaatctttt gttcactgcc gaaagcgagt ccttgagcga
     3421 agttttctgg tcgataacct ggaaaatgcg ctcattggga attaccatat acgcatttac
     3481 gaagggctta attttttcca gcgcggcttc tgctatctgc tttcgtcgtt ctccttcgaa
     3541 cgcaaaaggc atggtaaaaa tacctatgca aaggttgcgc agctcctgtg aagcttctgc
     3601 aaacacatgc gtggctcccg atcccgtgcc tcctccaagc gaagctataa gaatgcagat
     3661 gtcgttcccc tcgagcaatt ttttaatccg ctctttttcc gctttcgccg cccgctcacc
     3721 caattcaggg tccatgccgc atcccaaacc attggttagc tcgtgcccaa aagaaatagt
     3781 ctttactttt ttgggaagtt cttttaacgc ctgggcgtcg gtgttggctc ccacgaagtc
     3841 gaatttgtgc accctggaag caatttcggc aacaatgttg ccgcccccgc cgcccacgcc
     3901 aattacttta attctggtgc ggtgaacctg atccacaggc aaaacctctt tgcctttggg
     3961 gcttttgtgt ttaccctgag atcgtcgaag ggcctttcta gccggctttt ttgtgagaga
     4021 ttttttcttt gccattttac tttaagtgta gggttgagca cgccttttgt caaatagaaa
     4081 agccggggag atatattccc cgaccatgag cttttcttga ctatcgagct gcggttgatg
     4141 gattcagctc aacatgcgca cgggcagaaa cgccctgcac tggcgcactt ttggcctttt
     4201 gccgggccat cgctgc
//
LOCUS       DASRUP010000063         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2034810, whole genome shotgun sequence.
ACCESSION   DASRUP010000063 DASRUP010000000
VERSION     DASRUP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034810"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..690
                     /locus_tag="VFE94_02485"
     CDS             178..690
                     /locus_tag="VFE94_02485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX49998.1"
                     /translation="MLLAVLFSIAANLVAITILASLVVSLIRGAPPVPSNKRTIEIFL
                     QIATEQVPQRAVDLGSGDGRVVQALAAHGIETHGYEINPLLVWWGKMRLRKAGLRHKG
                     VLKTANLWSQNLGEFDLVFIYGLPNVMRHFEAKLERELRPGARVASNSFPLPNWKPVK
                     QIEHVYLYQK"
     gene            722..967
                     /locus_tag="VFE94_02490"
     CDS             722..967
                     /locus_tag="VFE94_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZX49999.1"
                     /translation="MFDKLKQLKELKSLQDDLKKESYDIEKNGVRLVMNGGMELEEIR
                     LNPQLSQEQQEQAIMQAYNELIRKVQMAMAQKFRGMM"
     gene            complement(1323..1895)
                     /locus_tag="VFE94_02495"
     CDS             complement(1323..1895)
                     /locus_tag="VFE94_02495"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZX50000.1"
                     /translation="MLSYYELRKGIQFIYEGQPYEVLEFRQMGKSQDVVVAQTKIRNL
                     LTGRVLPKNFHQGDTFQEADLEKFQAKFIFANRGKFTFARANDPGKRFELLEEQVGQA
                     AKFLKSNQEVEAIIFQEKVISITPPIKVQLKVKDAPPGVQGDRSAGGTKTVTLETGAV
                     INVPLFIETNDIIELNTETGEYARRIEKGE"
     gene            complement(1888..>2419)
                     /locus_tag="VFE94_02500"
     CDS             complement(1888..>2419)
                     /locus_tag="VFE94_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HZX50001.1"
                     /translation="PTPVPFTILNGEIETGVSIIKMDEEVDHGPLLAQREFSIINYQF
                     SNGLGKPTTPELLQVLWDMGAELLVEILPKYIAGEIMAQEQDHSQATFTKKLTREDGL
                     IDWKQNPEYIDRQIRAFTPWPGAFTFWKGKRVKILKAHVEEDKLVVDELQMEGKKPTT
                     LKEFLLGYPDFKIPYA"
BASE COUNT          630 a          581 c          559 g          649 t
ORIGIN      
        1 aaatgtcaga ttcgaagaaa gcattcccga agccttgaaa agagtagagg caatgggggc
       61 gccccgcctc gtgttcgacc ctatgagcct ttcttcgcca ggaggcattg tggtgcctgc
      121 catgaaaatc aagaactttc gtttggtggg ttctacgaaa cggtcgatgt aacatatatg
      181 ctgctggcag tactattctc tatagcggca aatcttgtag cgatcactat tttagcttcg
      241 ctcgtagttt ctcttattcg cggcgcgcct cctgtgccca gcaacaaacg cactatcgag
      301 atttttctgc aaatcgccac cgaacaagtt ccccaaaggg cagtagactt gggatcaggc
      361 gacggcagag tggtacaagc gctggcggca cacggtattg aaacccacgg atatgaaatt
      421 aacccgttgc tggtgtggtg ggggaaaatg cgtttgagaa aagcgggttt gcgccacaaa
      481 ggagtcttaa aaaccgcaaa tctttggagc caaaaccttg gagagtttga tttggtattc
      541 atttacggct tgcccaatgt tatgcgccat tttgaagcaa agctcgaaag agaattgcgg
      601 cccggagccc gggttgcttc aaactccttt ccgcttccaa actggaaacc ggtcaaacag
      661 atcgaacatg tctacctcta tcaaaagtaa aagaaataag ctataatccc accaaagaac
      721 tatgttcgat aaattaaaac aactcaaaga gttaaaaagc ctgcaagacg acctaaaaaa
      781 agaaagctac gacattgaaa aaaacggcgt gcgccttgtt atgaacgggg gaatggagct
      841 tgaagaaatc aggctcaacc cacaactttc ccaggaacag caggagcagg ccataatgca
      901 ggcttacaac gaattgattc gtaaggtgca aatggccatg gcgcaaaaat tccgcggcat
      961 gatgtagtct gcaaaagttt tccacaccct tgaactttct ctgaaatttt aaaaatatgc
     1021 tataatttga aaatctaaat aggggaggac atagcttgca gaaacactgg tggaataagt
     1081 gtccctgtgg atccaagttt gacctcgagt gtgcaatatg ccccagctgc aaacgcgaag
     1141 gcccgttcca tcaactcttt ttgagcaatg gggcagtcct cgcagaccct tcaaaagttc
     1201 tttccgcgca tatgtcccgc ctttacccag gccccaacca tgccgccgaa ggatactatg
     1261 cggcatccct agaatacctg gagaaaataa atggacggct ctcttgagtc gtcccgcctt
     1321 ttttattcgc ctttttcgat gcggcgggcg tattcgccgg tttccgtgtt tagctcgata
     1381 atatcgttag tttcaataaa cagggggaca tttataacgg cgccggtttc cagggttacg
     1441 gttttggtgc cgcccgcgga gcggtcgccc tgcacacccg gaggggcgtc ttttacttta
     1501 agctgcactt ttatgggcgg agtaatacta ataactttct cttggaaaat aattgcttct
     1561 acctcctgat tgcttttgag gaattttgca gcctggccaa cttgttcttc taaaagttca
     1621 aaccgcttgc cggggtcgtt tgccctggca aacgtaaact tgccgcgatt ggcaaaaata
     1681 aactttgcct ggaatttttc aaggtccgcc tcttgaaacg tatcaccctg gtggaagttt
     1741 ttgggcaaca cgcgcccggt tagcaagttg cggattttgg tttgtgccac taccacgtct
     1801 tgcgattttc ccatttggcg gaattccaaa acctcgtagg gctgcccctc gtatataaat
     1861 tgaattcctt ttcgtaattc gtaataacta agcatagggt attttaaaat caggatatcc
     1921 gagcaaaaac tcttttaggg ttgtgggttt tttgccttcc atctgcagct cgtctacaac
     1981 cagcttgtct tcctccacat gcgctttcag gatttttacg cgcttgcctt tccaaaaagt
     2041 aaatgctccg ggccagggcg taaacgcccg aatctgccta tctatatact ctggattttg
     2101 cttccagtca ataaggccgt cttcccgggt aagctttttt gtaaaggtag cctggctgtg
     2161 atcttgctct tgggccataa tttcacccgc aatatatttg ggcaagattt ctaccagcaa
     2221 ctcggcgccc atgtcccata aaacctgcaa cagctctgga gtagtaggct tgccaagccc
     2281 atttgaaaat tgataattga taattgaaaa ttcccgttgc gccagcaacg gcccgtgatc
     2341 aacctcttcg tccattttaa taatgcttac tccggtttcg atttcgccgt ttaaaatggt
     2401 aaagggcacg ggcgtggga
//
LOCUS       DASRUP010000064         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1961269, whole genome shotgun sequence.
ACCESSION   DASRUP010000064 DASRUP010000000
VERSION     DASRUP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961269"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFE94_02505"
     CDS             <1..484
                     /locus_tag="VFE94_02505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZX50002.1"
                     /translation="IYLESDLFSKGQRPAVNIGLSVSRVGSAAQTKATKKVASQLKLD
                     LAQFEELAAFAQFAQDLDKATKAKIDRGQRLLEVLKQGQFQPIPMEEQVVALFAANEG
                     FLDAVLLDKVRDFEKKLVQHLKDHHAKVLQTIRESRDLADDTKAELKKATEKFVASYK
                     "
     gene            509..1066
                     /locus_tag="VFE94_02510"
     CDS             509..1066
                     /locus_tag="VFE94_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50003.1"
                     /translation="MNITKYMLLGLAIFIIVGGAAYYFTQIQNRAIDTSNWPVYQNDE
                     YAVYEIKHPEGAEVSEGAGGTRIDLPFEEETTLVEKYFQIGYTGQDSEAKCSSAHLGQ
                     VFASEMVSINGLEIHRETGAEEIPRAKFDYVGYTVGKDGLCYQMKFVLRSVTEGVSGE
                     ELEQFDKEQEMKFVEAMIQTFRFTN"
     gene            1071..2030
                     /gene="atpG"
                     /locus_tag="VFE94_02515"
     CDS             1071..2030
                     /gene="atpG"
                     /locus_tag="VFE94_02515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HZX50004.1"
                     /translation="MAATRQLKTKIRAVKNIGQITRAMQMVAATKMRKSQEVALNARP
                     YAKKSFALLLHLLKQAGKEETESIFWPAKGGTVPPLEKVALVVITSDKGLAGSFNSSV
                     LRAAYKFVQDETVRSTASNMEIVAVGKKGRDFFKARGAVIAAEFLQFSDIVTYDNVRP
                     LADWIVQAYETQQYSKIVVCSNQFVSALVQKVEIRQILPLEIEALQQTIEGIVPKTGR
                     YSELAAGEKETEKELSSVLEPSREEIIKNLVRNLIQVQIAYFVFESNASEHSARMVAM
                     KNATENAKRLQEELTLEWNKARQAAITQELTEISTAKEALTAE"
BASE COUNT          642 a          414 c          550 g          481 t
ORIGIN      
        1 aatttatttg gaaagcgatt tgttttccaa gggccagcgg cccgcggtaa atattgggct
       61 ttcggtttct cgcgttggtt ctgcagccca aacaaaagct accaaaaaag tggcgtcgca
      121 gttaaagctg gatttggcgc aatttgaaga attggcagct tttgcccagt ttgcgcagga
      181 tttggacaaa gcaaccaaag ccaaaatcga ccgaggccag cgtttgctgg aagtgttaaa
      241 gcaggggcaa ttccagccta ttcctatgga agagcaggtg gtggcgctgt tcgcggcaaa
      301 cgaaggcttt ttggacgcgg ttttgctcga caaagtgcgc gattttgaaa agaaattggt
      361 gcagcacctc aaagaccacc acgccaaagt tctgcaaacc attcgcgagt ctcgcgacct
      421 tgccgacgac accaaagcgg aactcaaaaa agcaacggag aaatttgttg caagctataa
      481 ataacaagta attcccaatc aacaaccaat gaacatcaca aaatatatgc tcctcgggct
      541 cgctattttc ataattgtag ggggcgcggc atattacttt actcaaatac aaaatagagc
      601 aatcgatact tccaattggc ccgtgtacca aaacgatgag tatgccgttt acgagattaa
      661 gcatcccgaa ggggcagagg ttagcgaggg agctgggggc acgcgcattg atttgccgtt
      721 tgaagaagaa accacccttg tggaaaaata tttccagata gggtacaccg gacaggattc
      781 agaagccaaa tgttcgagtg cccacctagg ccaggttttt gcttcagaaa tggttagtat
      841 taacggattg gagatccata gggaaaccgg agcagaggaa attcccagag caaagtttga
      901 ttatgtgggg tatacggttg gcaaagacgg gctttgttac caaatgaagt ttgtgctgcg
      961 atcggttacc gagggggtaa gtggcgaaga gcttgaacag ttcgacaaag aacaggaaat
     1021 gaagtttgtt gaggcaatga ttcaaacctt ccgatttacc aattaataac atggcggcaa
     1081 cgaggcaact gaaaacgaaa atacgggcgg taaagaatat tgggcagatt acgcgggcca
     1141 tgcaaatggt ggctgccacc aaaatgcgaa aatcgcagga agtggcgctt aatgccaggc
     1201 catacgcgaa aaaatcgttc gcgctgctgt tgcatttgct aaaacaggcc ggcaaagaag
     1261 aaaccgagag tattttctgg cctgcaaaag gagggacagt ccctcctttg gagaaagttg
     1321 ctttggtggt tattacgagc gacaaggggt tggccggaag ttttaacagc tcggtgttgc
     1381 gcgcggcgta taagtttgtc caagacgaga ctgttcgaag tacggcttca aatatggaga
     1441 ttgtggcagt tggcaaaaag ggccgcgact tttttaaggc aagaggcgcc gttatagccg
     1501 cggaattttt gcagttttca gacattgtta cttatgataa tgtaaggccg cttgcagatt
     1561 ggattgtgca ggcatacgaa acacagcagt atagcaaaat cgttgtctgt tcaaatcagt
     1621 ttgtatcagc tttagtgcaa aaggtagaaa tccggcaaat tttgccgctg gaaattgagg
     1681 ctttgcagca aacaatagaa ggaattgtgc caaagacagg tagatactcc gagcttgcgg
     1741 caggggaaaa agaaacagaa aaggaattaa gctctgtgct tgaacccagc cgtgaggaga
     1801 ttatcaaaaa cctggtgcga aatttaatac aggtgcaaat tgcgtatttc gtgtttgagt
     1861 caaacgcttc agaacattct gctcgcatgg tagccatgaa aaacgccaca gaaaacgcaa
     1921 agcgcctgca agaagaactt accttggaat ggaacaaagc gcgccaagct gcaattacgc
     1981 aagagcttac ggaaatttca acagcaaaag aagcattaac cgcagaataa tatgaataaa
     2041 ggtaatattg tacaaattac agggccggtg gtggatgtcg aatttga
//
LOCUS       DASRUP010000065        18554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1196672, whole genome shotgun sequence.
ACCESSION   DASRUP010000065 DASRUP010000000
VERSION     DASRUP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18554
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196672"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..237
                     /locus_tag="VFE94_02520"
     tRNA            154..237
                     /locus_tag="VFE94_02520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:188..190,aa:Leu,seq:caa)
     gene            complement(328..609)
                     /locus_tag="VFE94_02525"
     CDS             complement(328..609)
                     /locus_tag="VFE94_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZX50005.1"
                     /translation="MKQMKKIPQFKNEEQERAFWAKEDSADYIDWSKAKRVSFSNLKP
                     STKTISLRLPEHLLEKIKRTANQQDIPYQSLIKYSLAQRFLQRKNKVEK"
     gene            complement(578..886)
                     /locus_tag="VFE94_02530"
     CDS             complement(578..886)
                     /locus_tag="VFE94_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZX50006.1"
                     /translation="MTDIPHFTGFQWDEGNRDKNWTKHKVSNSECEEVFFNESLIVPD
                     IKHSEQEQRYYALGRTNEERLLFISFTIRENLIRVISARAAGRKEKQQYETDEKNSTI
                     "
     gene            complement(1056..2279)
                     /locus_tag="VFE94_02535"
     CDS             complement(1056..2279)
                     /locus_tag="VFE94_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50007.1"
                     /translation="MDQQSQQPNQSLNPTPFDGPKQGGHKKVKIALFVLVAVLVVIGA
                     IFFIIFNKSVGINPVAYAQCDQLEQEIDTLVKQANYCEVDEDCVVSSEAVYPLCSCWS
                     LVNKEVDTANLKEKRSEWEEAGCRRKDVVCEQCAQPPQILKCEQSRCVGTYYRPTTMQ
                     ELKGYPELNKVMPELLNGHTVMVDGIYRWGFEDSNFGETADGYDIWVEMIADTEFENR
                     PENFYGVEKSAPSRTAKVTIYGTFQTGNLERSLNFGHLGLWEHQIVADKIVFEETAID
                     ENLVRIMTDKSTYRQGEVVRITAKNNYETTKWLEPYPYAIERFVGRSWVAVSQVNCPC
                     GALCNAPPVTPLVPNEQVGFLWNQEEESCSTNFQLVSNVAPAGKYRVKVLVRNDINDE
                     QTRETVYSEEFIIED"
     gene            complement(2368..4719)
                     /locus_tag="VFE94_02540"
     CDS             complement(2368..4719)
                     /locus_tag="VFE94_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZX50008.1"
                     /translation="MEITDFEKGGKRQVFKPVFGSIAELLEHHAKEFGKKEAIVSVNV
                     DSGQERSISYAKLLELAQKAACFLAKKGVQKGDRVAFAFENTSTILVLELAAGLLGAS
                     SVPIDVKRDTPERKEFKLKDANAKVFFEDEQEVLEALEKQSDQNPPLSSGSTKDEYLL
                     LYTSGTTANPKGVSLSLRAMMANAQGIAEWQKLSPNDRFGIVLPLHHINSTTMSLATL
                     LAGGTIVLSSRYSASKFWQVASKHKVTITSIVPTILHDLLARKEEYSAKKYPLAFKRI
                     LIGSAPVLPEETMRFMETFAIDVVQGYGQTETALRVTGVPIGLPRKLYRELTRKNSIG
                     KELAYCNVTILKEDGSGSTEKEEGEICIRGPVVGDGYWNNKEATEKDFSAGWFHSGDL
                     GYYEIIAGEKYFFIKGRIKEIIIKGGVNISPAAVEDALLKNFAEINEVCVVGYEDSRM
                     GEEVAAVITLVHPEENGDIAEKIVRFGGEGKLVGLSRYEAPVKVFVLDEELPKTSTGK
                     IQRVKVKDMVQARVAAEKQQHLYCRLIEPREKAIIAKAVEVNNKRWPVASSVEEFEQR
                     AKNGYLIGVFDEEESLLGTLSALQMSGDALEAAKTWDQATNNGTLQNNNPKGEIMVCA
                     SITVTDEARGGTVPPRAEVSQEVADEVIEEYIKTGLDNVLRFHAKPKGGMEKGAKVIK
                     ILPQGRPEDTEAMGYNVLVEYPTIDKRKKIVRSKNAPPSFLLIEQTMQLAKEKGCKRV
                     VAFSRPAQFKLHLVKSLDPSINFEPKDSKVFTKFATLAQKKNG"
     gene            complement(4790..6292)
                     /locus_tag="VFE94_02545"
     CDS             complement(4790..6292)
                     /locus_tag="VFE94_02545"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HZX50009.1"
                     /translation="MNNLVFLNDIAIQLSSYLPTLIGALAILLLGWIIGLVVAGIVRG
                     ALRRTTVDEKIAGWLLGSESVRGIEVERWIAKAVFYLVLLFTLVAFFETLGLTLIADS
                     LDQFLGEIGAYIPRFIEAAFVLALAWVVASGARMVIKRVLGLAKIDSYLEMQAGVRRE
                     KRTPLAKTLSDAVYWLVFLLFLPALLDALEVGGLLGPVQGMTNEILSFLPNILAAGLV
                     LLIGWFVARIVERIATNLLMSVGVDQIAERVGLGQVLGSQTLSGMLGRIAYVLVLIPV
                     LIASLHALGLEAITQPASNMLNIILAAVPSIFAAVLVMAFAYILGRIVGGLVTNLLAN
                     IGFDKVLVMLGFGEESSSRWKPSDVVGYLALVAIILFAFVESLSLLGFGVLANLTAEF
                     IVFSGHIILGVVIFAIGLYLANVASNTVAKSKNKKADFLALAAKISILVFAGAMALRQ
                     MGLANDIINIAFALVVGAVAVAFVLALGLGGRDVAAAHLEEWVKAFKKKR"
     gene            complement(6436..7254)
                     /locus_tag="VFE94_02550"
     CDS             complement(6436..7254)
                     /locus_tag="VFE94_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50010.1"
                     /translation="MLISVVLIGVAAALYFLWYVPGVRAQEYIEKSRPIFERITTQTF
                     TVKENTFLDRNTQYHKREDARDDAILDIEELEAAAENIAQAQAEIGQLPIPKKIVPLS
                     DRVNAYLAEAERVVSLNFADQVFYKKVIDAYGDDLDREISVYDEMRYSGSDRTPFITQ
                     TQSVADYAQDALSRINNLEVPEGVDPFAYEIRKENLEDIKETFLKFNEYYRLFQYELI
                     GPEVEANTKRNEERNRQIREHGQVYITESNIAQGFKSLEQQSQELKNLYEQVNL"
     gene            complement(7405..8235)
                     /locus_tag="VFE94_02555"
     CDS             complement(7405..8235)
                     /locus_tag="VFE94_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HZX50011.1"
                     /translation="MAVEILDGKKVAQEMLRDVKERAKGKSLKLAVIQVGKNKVSETY
                     IAEKQKVGAEAGIAVQVFWLSPDITQEGLEQEVTQISTDPNTHGVIVQLPLPKHINRE
                     EVLSKIPLEKDVDVLSETALEQFKENTFPVLPPTVGAVQALFSAYGVEIRGKKVVLVG
                     HGRLVGVPLGIWLKNQGVQFEIADKATKDLSALCRTADIIISGVGIPKLITGDMIKQG
                     AVVIDAGTSVESGKTSGDVDFVSVSAKASFITPVPGGVGPLTVACLFHNLVTLADKRV
                     "
     gene            8285..9106
                     /locus_tag="VFE94_02560"
     CDS             8285..9106
                     /locus_tag="VFE94_02560"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HZX50012.1"
                     /translation="MIGKKTSKLDSKKRYLQIALNSTLKEAGSIISQLPVSDRILVEA
                     GTPFIKRYGVEGIKQLKKLYTLRIAGEKIHGQLEEFPVQGFGGLSVFFSKLAELNRQS
                     QPASQKLQFKLPFNPYVVADLKMMDRGATEVEIAQDGGADAAVALGHAPIESLDAFIA
                     HCSDVGLDSMIDMMNVEFPLSVLRKLKSPPNVVLLHRGVDEEHYNRQKQLPLHEIRRI
                     KSNYDTLIAIAGGDTHREVQSAIFNDADIVVVWKSFFHSTQHTAQLAEEFLREIK"
     gene            complement(9120..9884)
                     /locus_tag="VFE94_02565"
     CDS             complement(9120..9884)
                     /locus_tag="VFE94_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZX50013.1"
                     /translation="MAILLGVAIILVFLVFAGLRVIDQYERGVVLTLGRYTGTRPPGL
                     TWLLIGIQRMTKVDLRVDTVDIPQQEVITKDNVTVGINAVVYFRVANAEKAILEIQNF
                     RRAVSLYAQAALRDVIGGVELDTLLSERDKIADEIKKIVDVATDPWGIDVSTIKIQDI
                     ELPADMKRVMAKQAEAERERRAIIIQAEGEFSAAEKLSQAANVLSNVPGGMSLRTLQT
                     IEKINPDPSKTVIFALPIEVTDTLKSLAQMFHERKG"
     gene            complement(9914..11146)
                     /gene="glyA"
                     /locus_tag="VFE94_02570"
     CDS             complement(9914..11146)
                     /gene="glyA"
                     /locus_tag="VFE94_02570"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005383582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZX50014.1"
                     /translation="MKTIQKQDKEIFDLIKKEEKRQVETVDLIASENYPSKAVREALG
                     SIYVAKYSEGRPFKRYYGGMENVDKLETLVEERVRRAFGIGQEWAVNVQPYSGSPANY
                     AVYRGLLEMGDTILSLDLSHGGHLTHGSPVSLSGMDFRIVHYQVNKKTQQLDYRAIAQ
                     LAEKEKPKLIIAGYSAYSRTIDFAAFGKIAQKAGAYLMCDIAHITGLIVGKAHPSPFP
                     HADVVTTTTHKTLRGPRGAMIVCREELREKIFPKVFPGLQGGPHNHTIAALGIALKEA
                     TTPAFRTYAAQIVKNANALAAALQTHDFNIVSGGTDNHVMLVDLRNKNIKGKQAQELL
                     ESAGIVANRNTIPFDPNTPFNPSGVRFGTPAVTTRGMKEKEMKQIASWIAEVIDDPKA
                     APKVKKEVAGLCKKFPIP"
     gene            complement(11143..11700)
                     /locus_tag="VFE94_02575"
     CDS             complement(11143..11700)
                     /locus_tag="VFE94_02575"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZX50015.1"
                     /translation="MASVQEATEMIKKGKVVVCPTDTVYGLVADATNEKAVKRIFQIK
                     GRGKGKALPVFVKNVAMAKKFARVSKEQEEFLRKVWPGKVTVVFQSKHVLPKALEMDG
                     KVALRIPKHELVAALLKKLNRPLTGTSANVSGQPSCSSATEVNVQFARRKHQPDLVVD
                     EGKLPKSKPSKIVDITGEKMRILRR"
     gene            complement(11714..12880)
                     /locus_tag="VFE94_02580"
     CDS             complement(11714..12880)
                     /locus_tag="VFE94_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZX50016.1"
                     /translation="MQAVVIAAGESKRFWPLNNGIHKSQFKLLGKPLLYWTLKGLAEN
                     GIKEVCIVVSKNSSIQEMLAQENNLDMNVSYVVQEKPLGTGNALWQAKNFIKEPFVLL
                     WGNKVGSKELVAQMIAKQKEGAEAVLVGNEVQNPSEYGVARMEGDRVMEIIENPKEGE
                     EPSHLAIRGARLLGPDFFEYYEKLPKHHEADIINATNAYLKDKKTALLIMQEQEMTLK
                     YPWDLFSVMDYLFATQKPVISPRAKIGENVVLDGPVFIGDNCVIGHGNVIRGPVNIEA
                     NCKTGAFMEIKHSIIQEGTVLHSGYAGDSIIGKNCRFGAGFITGNLRLDKEPIHGLPK
                     LGVIVGDNSIFGIHSGTMPGVLVGANCKIGPATHVFENLEDNTTFYAKFDSVKK"
     gene            complement(12905..13342)
                     /locus_tag="VFE94_02585"
     CDS             complement(12905..13342)
                     /locus_tag="VFE94_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX50017.1"
                     /translation="MQLRKADFNDKQKILQLANLLYIEMPDFVWNTEDYIVRQIDRGE
                     YFVAEEGGGIVGIISLRVRNGKMYIETLAVAPERQSDGLGTTLIEFAKHFAKEKGFGA
                     LCACAFFEYQNKDFYLKKGFSLLPWTGEYSGHQFHRFEIKLDF"
     gene            complement(13345..14295)
                     /locus_tag="VFE94_02590"
     CDS             complement(13345..14295)
                     /locus_tag="VFE94_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HZX50018.1"
                     /translation="MAKEKKIIVIGGGTGTYTALVGLKKYPVDLTAVVSMADDGGSTK
                     TLREEFGVLPPGSVRPALVALSHAPQAISNLFEFRFAEGEGLKGHSLGNLFLTALAKQ
                     YGSFERAIEEAGRMLNIHGRVIPSTLDNCKLVAQLENGEIIRGETNIDVPKHDGSLKI
                     QKVWLDPQCAGNPKAMEALKTADIIVIGPGDLFTSLVPNLLVEGIADAVRESKAKKIF
                     VCNLMTKFGETSNFTASDFVEVLEQYIGKGTLSHIIVNDKKPDDDRIAKYEKAHAHMV
                     EYDKKQLKEKGLKVIEENVLRPVGLIRHNSDTLARIICKL"
     gene            complement(14297..16126)
                     /gene="glmS"
                     /locus_tag="VFE94_02595"
     CDS             complement(14297..16126)
                     /gene="glmS"
                     /locus_tag="VFE94_02595"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZX50019.1"
                     /translation="MCGIVGYIGKKEALPLLMEGLRRESYRGYDSSGVCVFNGSEVVC
                     QKAVGKLEELEKKVSTQEIAGGIGIGHSRWATHGGVTEENAHPHTDCKGNIFVAHNGI
                     IENFKQLKDKLQKDGHVFQSQTDTEVLAHMVEQFFKGNLEDAVRKALTYIKGTYGLVV
                     IAKEDPGKIVAARLSSPLVLSINGSGGFVASDPAALIAHSNKMIFLDDREIAVIKEDD
                     FAVTDLENHEKKKEVTELDWTVEEAQKGGYEHFMLKEIMEQPESLRNALRGRLIEKEG
                     MVKLGGLESIETELRNTERLNIIANGTAYIAGLVGEYMLEEYAGIPVEVDYGSEFRYR
                     KPILNEKTASLFISQSGETADTLGALQEVKRKKGLSLGIINVVGSSVAREIDAGVYNH
                     AGPEIGVASTKAFTSQLAVLALLTMYLGRQRDMSLVTGQRIAKELALLPNLVQQTLEA
                     NKDRVKEIAEKYKNAKNFLYLGRKYNYPIALEGALKLKEISYVHAEGYSAGEMKHGPI
                     ALIDENFPTVVIAPTDSVYEKMISNIQEIKARKGPIIAVATEGDKEIVELADDVLYIP
                     KTLEMLTPILSVIPLQLFAYYIAVFHDLDPDKPRNLAKSVTVE"
     gene            complement(16155..16952)
                     /locus_tag="VFE94_02600"
     CDS             complement(16155..16952)
                     /locus_tag="VFE94_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50020.1"
                     /translation="MALVGAGAFLAALAFRPLPLANTQQAALVIQETGGMEASDSEEW
                     WVNSGAQVEKTESGFQTIQGELPWYSWWRIRYLLSNPVDTDNGEHPQNILRFVNKSKW
                     ENGQQTMYVRVHRLNMSASPERGAWSGIFFLHRYKDGDNLYYAGIRQDGAAVIKKKMN
                     GVYYTMAYAPWYKEEAPYNREINPNLIPGKQWIGLRTLITSNEDGSVLIRLLLDEEGN
                     GSWQTALEARDEGSQYGGDPIRGEGHGGMRSDFMDMEFRDYKVEPLS"
     gene            complement(17018..>18554)
                     /locus_tag="VFE94_02605"
     CDS             complement(17018..>18554)
                     /locus_tag="VFE94_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZX50021.1"
                     /translation="KKNELERTEVSKEKTGVELKGVKAINPGTKEEIPIWIADYVLAS
                     YGTGAIMAVPAHDERDFEFAQKYNLPIIMVVCPHYPAKTCPVMDEAYLGEGHLVASGK
                     FDGMPNEKAKGEITKAVGGTKKTNYHLRDWLVSRQRYWGAPIPIVYDPEGKPHAVPEK
                     YLPWMLPTDAEFKPTGYSPLADSKEFVRRTEKIFGKGWKPEVDTMDTFVCSSWYYFRF
                     ADPANGKEFASKKAIQQWLPVDVYMGGAEHTVLHLMYARFFTKVLRKLGYVDFVEPFW
                     KLRHQGIILAENGQKMSKSKGNVVNPDDIIEEYGADTLRMFEMFLGPLEDMKPWNTKS
                     IVGLYRFLNRIWNFQFSISNFQANPKKDLENLRHKTIKKVTEDIENLRFNTAIAALME
                     YSNALGETMNKKDVETLLILLAPFAPYMTEELWQQMGNKGSVHSQKWPQHDAKKIQET
                     KIRLILQVNGKVRDMVEADANIKEAEARELALQNEKMQKWLEGKQPKKVIFVAGRLVN
                     VVV"
BASE COUNT         4538 a         4793 c         3798 g         5425 t
ORIGIN      
        1 tgggacaagc ccacgggcga gaaatgccaa acctgcaact cgcttatggt agaaaaagcc
       61 aaagccgcca aatgctccaa caaagactgc gaaaccaaca aaaagaagta gtttacaaac
      121 cccaaattct ctggtaaaat aggctctgca catgcccggg tggcggaatt ggcagacgcg
      181 ctggactcaa aatccagtga gggcaacctc atgagagttc aagtctctcc ccgggcactt
      241 cgattaagaa aaacggctgt tagccgtttt tctcttttac gagttttgcg aatttggaga
      301 aggtttgcgg gtcgcggggt tcaaaatcta cttttctacc ttgttttttc gttgcagaaa
      361 tctctgcgca agcgagtact taattaaaga ctgataagga atatcctgtt gattagcagt
      421 acgcttgatc ttctctaaca aatgttcggg taagcgaaga gagatcgttt tggttgacgg
      481 tttaagattt gagaaagaaa cccgtttcgc tttggaccag tctatatagt ctgcagaatc
      541 ttctttggcc caaaaggcgc gttcctgttc ttcgttttta aattgtggaa tttttttcat
      601 ctgtttcata ttgttgtttt tcttttctgc ctgcagcgcg ggcagaaatt actcttataa
      661 gattttctct aatggtgaac gaaataaata gtagtctttc ttcatttgtt cttccaagag
      721 catagtatcg ttgttcttgt tccgagtgct ttatatctgg cacaatcagt gattcgttaa
      781 aaaagacttc ttcacattca ctgttggaaa ctttgtgctt tgtccagttt ttatctctat
      841 ttccttcatc ccattgaaac ccagtaaaat gtggtatatc cgtcatatag ggttactact
      901 tgtatatctc tactatatac aagcaaaggt gcgttgtcaa tcccttactc cgcaattacc
      961 ctagctcttt ttacgagttt tgcgaacttt gcaaaggttt tttggtcttt gggttcaaaa
     1021 tttatggagg ggtccaggga tttggcgagc agtacctaat cttctattat gaactcttca
     1081 gaatatacgg tttctcttgt ttgttcgtcg tttatatcgt tcctgactaa gaccttaact
     1141 ctatacttgc ccgcgggtgc tacgtttgat accaattgaa aattcgtaga acaagactct
     1201 tcttcttgat tccacagaaa ccctacttgc tcatttggca ctaagggagt cactggcgga
     1261 gcgttgcata aagcaccgca gggacagttg acttgtgaaa cagcgaccca actacgtcct
     1321 acaaacctct cgatagcata tgggtatggc tcaagccatt ttgtagtttc gtagttgttc
     1381 ttggctgtaa tgcgtactac ttctccttgc ctgtatgtgg atttatccgt cattatccga
     1441 acaagatttt catcgatagc tgtttcttcg aacacaatct tatctgccac gatttgatgt
     1501 tcccagaggc cgagatggcc aaagttcaaa ctgcgttcca gatttcccgt ttggaatgtg
     1561 ccgtagatgg tgacttttgc cgttctgctt ggcgctgatt tttccactcc ataaaaattc
     1621 tctggtcgat tctcgaattc cgtatccgca atcatctcaa cccaaatgtc atatccatct
     1681 gccgtttcgc caaagttgct gtcttcaaac ccccaacggt agattccatc aaccatcacc
     1741 gtatgaccgt tcagtaactc aggcataacc ttattcaact cggggtaacc cttgagttct
     1801 tgcattgttg ttggcctgta gtatgtgcct acacagcgac tctgttcgca tttgagaatc
     1861 tggggaggtt gagcgcactg ctcacacact acatcttttc ttcgacatcc ggcttcttcc
     1921 cattcagacc tcttttcttt taggtttgcc gtatccactt ctttattaac aagactccag
     1981 caagagcata gtggatatac tgcttcagag ctcaccacgc aatcctcatc cacctcgcag
     2041 tagtttgcct gtttgacgag tgtatctatt tcctgttcaa gttgatcgca ttgggcataa
     2101 gccaccggat taataccaac ggatttattg aaaatgatga agaaaatcgc gccaatgact
     2161 acaagaacag ctacgagaac aaacaaggct atttttactt tcttgtgccc tccttgtttt
     2221 gggccatcaa acggtgttgg attcaatgat tgattgggtt gttgactttg ttgatccata
     2281 aaatcagtat aacaaagttt tgtatattgc aacaaggatg aaggtacgtt ccagcactat
     2341 cgtatagata caccaaaaaa cggctgctta gccgtttttc ttttgggcga gggtggcgaa
     2401 tttggtgaat actttactat ctttgggttc gaaatttata gacgggtcga gggatttgac
     2461 gagatgtaat ttgaattggg cggggcggga gaatgcgacg acgcgtttgc agcctttttc
     2521 ctttgctaat tgcattgttt gctcaataag caaaaaacta ggtggcgcgt ttttcgatcg
     2581 tacaattttc tttcgcttgt ctattgttgg atattctacc agcacattat agcccatggc
     2641 ttccgtgtct tccggcctgc cctggggcag aatttttata actttagctc ccttttccat
     2701 tcctcccttt ggctttgcgt gaaaccgcag cacgttgtct aacccggttt ttatatattc
     2761 ttcaataacc tcatctgcca cctcttgcga aacctctgca cgaggaggga ctgtccctcc
     2821 tcgcgcttcg tcggttacgg taatagacgc gcataccatt atttcgcctt tgggattatt
     2881 attttgcaaa gttccgttgt ttgttgcctg gtcccaagtt tttgcagcct ccaaggcatc
     2941 gcctgacatt tgcaaagcgc ttagcgtacc taataaactt tcttcctcgt cgaatacgcc
     3001 aataagatac ccgttttttg cgcgctgctc aaattcttca acgctcgaag ccactggcca
     3061 tcgcttgttg tttacctcta ccgcctttgc gataattgct ttttcgcgag gttcaattaa
     3121 acggcaatac aaatgctgct gtttttctgc agccacccgg gcctgcacca tgtcttttac
     3181 ttttacacgc tgaattttgc ctgtagaagt ttttggcagt tcttcgtcca atacaaaaac
     3241 cttgacggga gcctcgtaac gcgaaagtcc aaccaatttg ccttcacccc caaaacgcac
     3301 aattttctct gcaatatccc cattttcctc cggatgcacc agggtaatta cagctgccac
     3361 ctcctccccc atgcgggagt cctcgtaccc caccacgcat acttcgttaa tttctgcaaa
     3421 attcttgagc aaggcgtctt ccaccgcggc cggggaaata tttaccccgc ctttaataat
     3481 aatttctttt atgcggccct ttataaaaaa gtatttctct cccgcgatta tctcataata
     3541 tccaaggtcg ccagagtgga accagcctgc agaaaaatct ttttcggtag cttctttgtt
     3601 gttccagtag ccgtccccga ccacggggcc gcgaatgcaa atttcgcctt cctctttttc
     3661 tgtagatcct gaaccgtctt ctttgaggat ggttacatta caatacgcaa gttctttgcc
     3721 aatgctgttt ttgcgcgtaa gttccctata cagtttgcgc ggcaagccaa taggcacgcc
     3781 ggtaacgcgc aacgcggttt cggtttggcc gtacccttgc accacatcta tggcaaacgt
     3841 ttccataaac cgcatggttt cttctggtag caccggggca gacccaatta aaatgcgctt
     3901 gaacgccaaa ggatattttt tggcagaata ctcttctttt cttgcaagca agtcgtgcaa
     3961 aatagtcggc acaatgctgg ttatggttac tttgtgcttt gacgccacct gccaaaattt
     4021 ggaagcagaa taccgcgagc ttaacacaat agtgccgccc gccagcaaag tggcaaggct
     4081 catagtggta gaatttatat ggtgcaaggg caatacaatg ccaaatcggt cgtttggcga
     4141 aagcttttgc cattctgcta ttccctgcgc gtttgccatc atagcgcgca aggaaagcga
     4201 cacgcctttt gggtttgccg tggtgcctga agtgtataaa agtaaatact cgtccttggt
     4261 cgaacccgaa gaaagtggcg gattttgatc ggattgtttt tccagggctt caagcacttc
     4321 ctgctcgtct tcaaaaaata cttttgcgtt ggcatctttt agcttaaatt ccttgcgttc
     4381 tggcgtgtcg cgctttacgt ctatgggcac gctggaagcg ccaagcaagc cggctgccag
     4441 ctccagcact aaaatagtag aagtattctc aaaagcaaaa gctacgcggt cgcctttttg
     4501 cactcctttt tttgctaaaa aacaagctgc tttttgcgcc aattccaaaa gtttagcata
     4561 cgaaatgctg cgttcttgcc ccgaatccac gtttactgaa acaatagctt cttttttgcc
     4621 aaattctttg gcgtgatgct ctagcaactc tgctatagac ccaaaaacag gcttaaatac
     4681 ctgtcttttc ccgccttttt caaaatccgt aatttccata ttatattgta ccgtatcatg
     4741 aaaaaaagag gcgctcaacc gagcacctct tttttcctga tattcgagtc tagcgtttct
     4801 ttttaaacgc ctttacccac tcttcgagat gggccgctgc cacgtcgcgg cctccaaggc
     4861 caagcgccag cacaaaagca accgcaaccg cgcccacgac aagcgcaaac gcaatgttaa
     4921 taatgtcgtt tgccaagccc atttgccgca atgccatagc tcccgcaaac accaaaatcg
     4981 agatttttgc cgccaacgcg agaaagtctg cttttttgtt tttgcttttt gccactgtgt
     5041 tggaagccac atttgccaga tacaggccaa tcgcgaaaat aaccactcca agaataatgt
     5101 gccccgaaaa cacgataaac tctgcggtaa gatttgccaa tactccaaaa ccaagcaagc
     5161 tcaaggattc cacaaatgca aacaggataa tagcaacgag cgccaaatat cctaccacgt
     5221 cggagggctt ccatcggctc gaactctctt cgccaaaccc cagcattacc agcactttat
     5281 cgaatccaat attggccaga aggttggtta ccaaacctcc tacaattctg cctaaaatat
     5341 aagcaaacgc cattaccaag accgcggcaa atatcgaagg aaccgcagcc aagataatat
     5401 tcagcatgtt tgaggccggt tgtgtaatcg cctccaaacc caacgcgtgc aaacttgcga
     5461 ttaatacagg gataagcacc agcacgtaag ctatccttcc caacattcca gagagggttt
     5521 gacttcccaa tacctggcct aagccgaccc gttctgctat ctggtctacc ccaacagaca
     5581 ttaacaaatt ggttgcaatt cgctccacta tcctggccac aaaccaccca atgagcaaca
     5641 caagccctgc agcaagaata ttaggcagga aactcaggat ttcgttggtc atgccctgca
     5701 caggccccag caatccgccc acctctaaag cgtcgagcaa agccggcaga aacaacaaga
     5761 acaccagcca atatacggcg tcagaaaggg ttttagccaa gggcgttctt ttttcgcggc
     5821 gcactccagc ctgcatctcc agatagctgt caatttttgc caagcccagc actcttttga
     5881 taaccatcct tgcgccagaa gcaacaaccc aagccagcgc caatacaaac gcggcttcaa
     5941 taaaccgggg gatataggct ccgatttctc cgaggaattg gtcgagcgaa tctgcgataa
     6001 gggttagccc gagggtttca aaaaacgcca ccagcgtaaa aagcaacaca agataaaata
     6061 ccgcttttgc gatccagcgt tctacctcga ttcctcttac cgattctgat cccaagagcc
     6121 aacccgcaat cttctcgtct actgtggtgc ggcgaagcgc tcctctcacg atgcccgcaa
     6181 ccaccaaacc aataatccaa ccaagtagca aaattgccaa agctccgatg agcgtaggaa
     6241 gatacgaact caattgaatt gcaatgtcat taagaaatac caaattattc atatatgttt
     6301 atttgtttct aacaaaatac tagtaaactt ttactcgacc tattatttca attatattat
     6361 tgtaccactc caaacaaaaa acagaaacat gttttccaca cgttcctgtt ttgcaaagaa
     6421 aatttgcact tctcgttaca gatttacttg ctcataaaga tttttaagct cttgagattg
     6481 ttgttccagc gacttgaatc cttgggcaat attcgactct gtaatataca cctggccatg
     6541 ctctcttatt tgtcggtttc gctcttcatt ccgtttcgtg tttgcttcaa cttctggacc
     6601 tatcagttcg tactggaaca atcggtagta ttcgttaaac ttcaaaaagg tttcttttat
     6661 atcttcaaga ttttccttgc gtatttcata cgcaaaagga tcaacaccct cgggcacttc
     6721 aagattatta attcttgata aggcgtcctg ggcgtaatca gccacgcttt gagtttgagt
     6781 aataaatggg gttcgatcgc ttcccgaata ccgcatttcg tcgtacacgc taatctctcg
     6841 atccaaatca tcgccataag catctataac ttttttataa aacacctggt ctgcaaaatt
     6901 taagcttaca actctttcag cttcagcgag ataggcgttc actcggtcac ttaatggaac
     6961 aatctttttt ggaattggaa gttggccaat ttctgcttgg gcctgcgcaa tattctctgc
     7021 cgcagcttct agttcttcaa tatctaaaat tgcgtcatct cttgcgtctt ctcgtttatg
     7081 gtattgagta tttctatcaa gaaatgtatt ttcctttaca gtgaaggttt gcgttgtaat
     7141 cctttcaaaa attggtcgag atttttcgat atactcttgc gcccgcaccc ctgggacata
     7201 ccacaaaaag taaagcgcag cggcaacccc aatcagaaca acgcttatca gcaaaacagc
     7261 tatccataag tacttttttc ggggtgaaac tttttgattt tgagcaaaac tctcgaccac
     7321 ttttccttgc tgcgcattat acattgaata atcttatcaa aaagtagcca aaaaggctac
     7381 tgttcgacca cgatacgaca aagcttaaac tcttttatct gcgagggtta cgaggttgtg
     7441 gaacaaacag gcgacggtga gggggccgac gccgccgggg acgggcgtta taaagctggc
     7501 ttttgcagaa acacttacaa agtcaacgtc gcctgatgtt ttgccagatt ccaccgaagt
     7561 tccagcatcg attacaactg ccccttgctt aatcatgtct cccgtaatga gtttgggaat
     7621 tcctacgccg gaaattataa tatctgcagt tctgcagagc gcagatagat cttttgttgc
     7681 tttgtctgca atttcaaact gcaccccttg gttttttaac catatgccca aaggcacgcc
     7741 taccaacctt ccatgtccaa ccagcacaac ctttttgccg cggatttcaa ctccataagc
     7801 agagaataga gcctgcacag ccccaactgt gggcggaagc acaggaaacg tgttttcttt
     7861 aaattgttcc aaagccgttt cgcttagcac gtccacgtct ttttccaacg gaattttact
     7921 gagcacttct tccctgttta tgtgtttggg aagcggaagc tgcactataa ctccgtgcgt
     7981 atttgggtct gtgcttattt gcgttacttc ttgttctaaa ccttcttgag taatatccgg
     8041 agaaagccaa aacacttgca ccgcaattcc agcctctgct cccacttttt gcttttctgc
     8101 aatataggtt tcagaaacct tgtttttgcc aacctgaatt acggcaagct ttaacgattt
     8161 tccctttgcc cgttctttaa cgtcgcgcaa catctcttgc gctacctttt tgccgtcgag
     8221 tatttcaact gccataggtg tttttggtat tcctcaaatt tagtataata aacccatctt
     8281 gtctatgatt ggcaaaaaaa cttcaaaact ggattccaaa aagcgctatc ttcaaatagc
     8341 gctcaacagc acgctcaaag aggcaggaag cattatctcg caacttcccg tgtcagaccg
     8401 cattttggtt gaagcaggaa caccctttat caaacggtat ggagtggaag gcatcaaaca
     8461 gcttaaaaag ctctatacct tgcgcattgc gggagagaaa atacatgggc aattagagga
     8521 atttcccgtg caaggatttg gcgggctttc ggtgtttttc tccaaacttg cagaactgaa
     8581 ccgccaaagc cagcccgctt cgcaaaaact ccagttcaaa ctgcctttta acccttatgt
     8641 ggtagcagac ttaaaaatga tggaccgcgg agccaccgaa gttgaaattg ctcaagatgg
     8701 gggcgctgac gccgctgtag ctcttggaca cgcccctatt gaatcgctcg acgcctttat
     8761 tgcgcactgc agcgacgtgg gcttagactc catgatcgat atgatgaacg tggaatttcc
     8821 cctgtctgtt ttgcgaaaac tcaaaagtcc tcctaacgtg gtactattgc accgcggcgt
     8881 cgacgaagag cattacaaca gacaaaaaca gcttcctctg catgagatac gccgcatcaa
     8941 atccaattac gacacgctta ttgcaatagc tggcggagat acgcaccggg aagtgcaaag
     9001 cgccatattc aacgacgcag atattgtggt ggtgtggaaa tcgttcttcc acagcacgca
     9061 acacacggcc cagctcgccg aggagttttt gcgtgagata aaatagttcg gcttctcccc
     9121 tacccctttc tctcgtggaa catttgggcc aaggatttta acgtgtcggt aacctcaata
     9181 ggaagggcaa aaataacggt ttttgacggg tctggattaa ttttttctat ggtttggagc
     9241 gttcgcagag acatgccgcc cggcacgttg cttaacacgt ttgccgcctg cgaaagcttt
     9301 tctgccgcgg aaaactctcc ttctgcctga ataataatgg cgcgcctttc gcgctctgct
     9361 tccgcctgct ttgccataac gcgcttcatg tcggccggca attcaatatc ctgtatttta
     9421 atggtggaaa cgtcgatccc ccaagggtcg gtggccacgt ctacaatctt tttaatctcg
     9481 tctgcaattt tgtcgcgttc agacaataaa gtgtccagct caacgccgcc gataacgtct
     9541 cgcaaagcag cctgcgcgta caggcttacc gcgcgcctga aattctgaat ttcaagaatc
     9601 gctttttccg cgtttgccac gcgaaaatac accaccgcgt taatgcctac ggttacattg
     9661 tctttggtaa taacctcttg ctggggaatg tctaccgtat ccacgcgcaa atccaccttg
     9721 gtcatgcgct gaatgccaat aagcagccac gttaaaccag gaggtcgggt acccgtgtat
     9781 cggcctaaag taagcactac tccccgctca tattggtcta tcacacggag ccccgcaaac
     9841 acaaggaaaa cgagtattat tgcaacgccc aacagtattg ccatatatct agattgtacc
     9901 aaacttcccc agtctaggga atgggaaact ttttacacag ccctgcaacc tcttttttta
     9961 ctttgggtgc tgcttttgga tcatcaatta cttctgcaat ccacgaggcg atttgcttca
    10021 tttctttttc tttcatgccg cgcgtggtta ccgccggcgt tccaaaacgc acgccagaag
    10081 gattaaacgg agtgttgggg tcgaaaggaa tggtattgcg gtttgccaca attcccgcgg
    10141 attccaaaag ctcctgcgcc tgcttaccct taatgttttt attgcgcaga tcaacgagca
    10201 ttacatggtt gtcggttccg ccagaaacaa tgttaaagtc gtgcgtctgc aacgcagccg
    10261 ccaaggcatt tgcgtttttc acaatttgcg cggcatatgt gcgaaaggcc ggagttgttg
    10321 cttctttgag cgcaatgcct aaagctgcaa tcgtatggtt gtgcgggcca ccctgcaagc
    10381 ccgggaacac tttaggaaat atcttctctc gcaactcttc gcggcataca atcatggctc
    10441 cccgggggcc gcgcaaggtt ttatgggtag tagtagttac cacatccgca tgagggaacg
    10501 ggcttggatg cgcctttccc actatcaacc ccgtaatatg ggcaatatcg cacataaggt
    10561 aagcgcccgc cttttgggca attttcccaa atgcggcaaa gtcgattgtt cgggaatacg
    10621 cgctataccc tgcaataatg agcttcggtt tttctttttc ggcaagctgg gcaattgccc
    10681 ggtaatccaa ctgctgcgtc ttcttattca cctggtaatg cacaatacga aaatccattc
    10741 cgctcaagct cacgggagaa ccatgcgtaa gatgccctcc atggcttaaa tccaaagaaa
    10801 gaatagtatc gcccatttca agcaagcctc gatataccgc atagtttgcc gggctaccag
    10861 agtatggctg cacatttacc gcccattctt gcccaatgcc aaaggccctg cgtacgcgtt
    10921 cctccacaag ggtttccagc ttgtccacat tttccatgcc gccgtaatat cgtttaaacg
    10981 gtctgccttc tgaatacttg gcaacgtata tggagcccaa agcttcccgc actgctttgg
    11041 aaggataatt ttcagaagca ataaggtcga cggtttcgac ctgccgcttt tcttcttttt
    11101 tgatgagatc aaaaatctct ttgtcttgtt tttggatagt cttcatcttc tcaagattct
    11161 cattttttcc cctgttatgt caacaatttt ggaaggtttt gattttggca gcttaccctc
    11221 gtcaaccacc aaatcgggct ggtgttttct tctggcaaac tgaacattta cttctgtggc
    11281 agacgaacac gaaggctggc cggaaacgtt tgccgaggtt ccggtaagcg ggcgattgag
    11341 ctttttcaaa agcgctgcca ccagttcgtg ctttggaata cgaagggcaa ctttgccgtc
    11401 catttccagc gctttcggga gcacatgctt actctggaac accactgtaa ctttgcctgg
    11461 ccaaactttc cgtagaaatt cctcctgttc tttggaaacg cgcgcaaact tttttgccat
    11521 ggcaacattt tttacaaaca cgggtaaagc cttgcctttt cccctgcctt ttatttgaaa
    11581 aatccgtttt actgcttttt cattcgttgc gtctgccacc aacccataca ccgtgtctgt
    11641 cggacacacc accaccttcc cctttttaat catttccgtc gcctcttgta cgcttgccat
    11701 tctgctatta tacctatttc tttaccgaat caaacttggc ataaaaggta gtattatcct
    11761 ccaagttttc aaacacatgc gtggcgggcc ctattttgca gttggcgcca accaacacgc
    11821 ctggcatagt gccagaatga atgccgaaaa tggagttgtc gccaacgatg acgccgagtt
    11881 ttggcaaccc atgaataggc tctttatcca atcgaaggtt tccggtaata aatccggccc
    11941 cgaacctgca gtttttgccg ataatagagt ctcctgcata tcctgaatgc aacactgttc
    12001 cctcctggat aatagagtgc tttatttcca taaaagcccc tgttttgcag ttggcttcaa
    12061 tatttactgg cccgcgaatc acgttgccat ggccgattac gcagttgtcg ccaataaata
    12121 ccgggccgtc taaaacaacg ttttccccga tttttgcgcg cggggaaata acaggttttt
    12181 gcgttgcaaa caaataatcc attaccgaaa acaaatccca cggatatttt aaggtcattt
    12241 cctgctcttg cataatcaga agcgcagttt ttttatcttt caaataggca ttagtggcat
    12301 tgataatatc cgcctcatgg tgtttgggca gcttttcata gtattcaaaa aaatcgggcc
    12361 caagtaaacg cgcgccccta atagcgaggt gagacggctc ttccccttct ttgggattct
    12421 caataatttc cataactcga tcaccctcca tccgcgcaac tccgtattct gaagggtttt
    12481 gcacttcgtt tcccacaagc actgcctctg ctccttcttt ttgcttcgca atcatttgag
    12541 ctaccaattc tttggaacct accttattgc cccacaaaag aacaaaaggt tcttttataa
    12601 aattctttgc ctgccacagg gcgtttcccg tacccaaggg cttttcctgc accacgtagg
    12661 aaacattcat atccaaattg ttttcttgag ccagcatttc ttgtatcgag gaatttttac
    12721 ttaccacaat gcacacctct tttatgccgt tttctgccaa cccttttaaa gtccagtaca
    12781 gcaaaggctt ccccaacagt ttgaactggg atttatgaat gccgttgttt aacggccaaa
    12841 acctttttga ttcgcctgct gcgataacaa ctgcctgcat gtgcctgata atagctaaaa
    12901 tgttttaaaa atcaagcttt atctcgaagc ggtggaactg gtggccgctg tattcaccgg
    12961 tccaaggaag caacgaaaac ccttttttca gataaaaatc tttgttttgg tattcaaaaa
    13021 atgcgcaggc acataacgcg ccaaacccct tttctttcgc aaagtgcttg gcaaactcaa
    13081 ttaaggtagt acccaagccg tctgattgcc tttctggcgc tacggcaagg gtttcaatat
    13141 acattttccc gttgcgtacg cgaaggctga taattcccac aattccgccc ccttcttctg
    13201 ccacgaaata ttccccccgg tcgatttgcc ttacgatgta atcctcggta ttccagacaa
    13261 agtctggcat ttcaatgtac agcaaattcg ccagttgcaa gattttttgt ttgtcgttga
    13321 aatcggcttt ccgcaattgc ataattagag cttgcaaatg atgcgggcta atgtgtctga
    13381 attgtggcga ataaggccta cggggcgcag cacgttttct tcaataactt tcaggccttt
    13441 ctcctttagc tgcttcttgt cgtattctac catatgcgcg tgcgcttttt catactttgc
    13501 gatacggtcg tcatcgggtt tcttgtcgtt tacgataata tgggagagcg ttccctttcc
    13561 aatatattgt tccagcacct caacgaaatc gcttgcggta aagttgcttg tttcaccgaa
    13621 cttagtcatt aagttgcaca caaatatctt ttttgccttg ctttctcgca ccgcatctgc
    13681 aatgccttct accaaaagat tcggcaccag gctggtaaac aagtcgccgg gcccaatgac
    13741 gataatgtcg gctgttttga gagcttccat ggctttcgga ttgcccgcgc actgcgggtc
    13801 taaccacact ttctgaattt ttaaactccc gtcgtgcttg ggaacgtcga tatttgtttc
    13861 tccccggata atttcgccgt tttccagttg cgccaccagt ttgcagttgt ccaaggtcga
    13921 aggaataacc cggccgtgaa tattcaacat acgccctgct tcttcaattg cccgctcaaa
    13981 actcccgtat tgtttggcaa gagcagtaag aaacaaattg cccaaactgt ggccttttaa
    14041 accttcgccc tctgcaaagc gaaactcgaa taagttggat atggcctggg gcgcgtgaga
    14101 taacgccact aaagctgggc gcacggatcc cggcggcagc acgccaaact cttcccgcag
    14161 cgttttggta gagcctccgt cgtctgccat ggaaactacc gccgtaagat ctaccggata
    14221 ttttttcagc ccaaccaaag cagtgtatgt tcccgtcccc ccgccgataa caataatctt
    14281 tttctctttt gccatattat tctacggtta cggatttggc gaggttgcgg ggtttgtcgg
    14341 ggtccaggtc atggaacacg gcaatgtagt acgcaaacag ctgcaacggg attaccgaca
    14401 aaatcggagt aagcatttcc agggttttcg ggatatacaa tacgtcgtct gctaactcta
    14461 cgatttcctt atccccttcc gttgccacgg caattatcgg gcctttcctg gcttttattt
    14521 cctggatatt tgaaatcatt ttttcataca cgctgtcggt tggcgcaatt acgaccgtag
    14581 gaaaattctc gtctattaag gcaatggggc catgtttcat ttcgccggcc gagtacccct
    14641 ccgcgtgcac atacgaaatt tcttttaatt ttaaagcgcc ctccaaagct atgggatagt
    14701 tatattttct gcccaggtat aaaaagtttt tggcgttttt atatttttcc gcgatttcct
    14761 ttactctgtc tttgtttgcc tctaacgttt gctgcaccag gttgggcagc aaagccaact
    14821 cttttgcaat gcgctgaccc gttaccaaag acatgtcccg ctgcctgccc aggtacatgg
    14881 taagcagcgc aagcacggcc aactgcgagg taaaggcttt tgtggaagca acaccaattt
    14941 ccggacccgc gtggttatac acgccagcgt ctatttcgcg cgccaccgaa gatccaacca
    15001 cgttaataat tcccaaactt aatccttttt ttctttttac ttcctgcaaa gccccaagcg
    15061 tatctgccgt ttcgcccgat tgtgaaataa acaacgaggc tgttttttcg tttagtatgg
    15121 gctttcggta gcgaaattcc gagccataat ccacttccac gggaatgccc gcatactctt
    15181 ctaacatata ttctcccaca agtcccgcga tatatgccgt gccgttcgca ataatattta
    15241 agcgttcggt gttgcgaagc tccgtttcaa tgctttccag tcctcccaat tttaccatgc
    15301 cttctttctc aattaacctg ccccgcagcg cgttccgcaa gctctcgggc tgttccataa
    15361 tttctttgag cataaagtgt tcgtatccgc ctttttgcgc ttcttccacg gtccagtcga
    15421 gttcggttac ttctttcttt ttctcgtgat tttccaaatc ggttaccgca aagtcatctt
    15481 cctttattac ggcaatctca cgatcatcca ggaaaatcat tttattggaa tgcgcaatga
    15541 gcgctgcagg gtctgaagcc acaaaaccac ccgatccgtt tatcgaaagc accagcgggc
    15601 tcgaaaggcg agctgccaca attttgcccg ggtcttcctt ggcaatcacc acaagcccat
    15661 acgttccttt tatatacgta agcgctttgc gtaccgcgtc ttccaaattg cccttgaaaa
    15721 actgttccac catatgcgcc aaaacctcgg tgtcggtttg cgattgaaat acgtgcccgt
    15781 ctttttggag cttatctttg agctgcttaa aattctcgat aatgccgttg tgcgccacaa
    15841 aaatattccc cttgcaatct gtgtgaggat gggcgttttc ttcggtaact cctccgtgcg
    15901 ttgcccatcg ggaatgcccg atgccaatgc ctcctgcaat ctcttgcgtg gaaactttct
    15961 tttctaactc ctctaacttt cccaccgcct tctggcaaac aacttccgaa ccattgaaaa
    16021 cgcaaactcc cgatgagtcg tacccacggt agctttcacg ccgcaacccc tccattaaca
    16081 aggggagggc ctccttttta cctatatatc ctacgatccc acacatacga acttttgtag
    16141 cacaaacaac aaagttatga aagtggctct actttgtagt cccgaaattc catgtccata
    16201 aaatcagatc gcatcccccc atgcccttcc ccccgaatag ggtcgccccc gtactgggag
    16261 ccctcgtctc gggcttccaa ggcagtctgc cagctgccat ttccctcttc gtccagtaaa
    16321 agccggataa gcaccgaccc gtcttcgttg ctcgtaatca gggttcgcaa accgatccat
    16381 tgctttcctg gaatgaggtt ggggtttatt tccctgttat agggtgcctc ctccttatac
    16441 cacggagcat aggccatggt gtagtatacc ccgttcattt tcttcttaat taccgcggcg
    16501 ccgtcttgac ggatacctgc ataatacagg ttgtcgccgt ccttgtacct atgcaagaaa
    16561 aagataccgc tccatgcgcc tcgttccggg cttgcagaca tgttcaaccg atgcacgcga
    16621 acatacattg tttgctggcc gttttcccac ttgctcttgt tcacgaaacg caaaatattc
    16681 tggggatgtt ctccgttatc ggtatccacg ggattggaaa gcaaatatcg aattcgccac
    16741 cacgagtacc acggaagctc cccttgaatc gtttggaatc cgctttctgt tttttcaact
    16801 tgcgcgccag aatttaccca ccattcttcg ctgtcgcttg cttccatacc ccctgtttcc
    16861 tggataacca gtgcagcctg ctgggtattt gcaaggggca aagggcggaa cgctaaagca
    16921 gccaaaaacg ctccagctcc cacgagggcc aaagttggta tagctctacg aattggtatt
    16981 tgcacacgta aactataacg cttgcccatt cgagatgtta caccacgacg tttaccaatc
    17041 gccccgcaac aaagataact tttttgggtt gcttaccctc gagccacttt tgcatttttt
    17101 cgttttggag cgcgagttcc cttgcttctg cttcttttat attcgcatct gcctccacca
    17161 tatcgcgcac cttgccattt acctgtaaga taaggcggat tttagtttcc tggattttct
    17221 ttgcgtcatg ctgcggccac ttttggctgt gcacactacc cttattgccc atctgctgcc
    17281 ataactcctc tgtcatataa ggagcaaaag gagcgagaag aatcagaagc gtttcaacat
    17341 ctttcttatt catggtttct cctagagcat tcgagtattc cattagggca gcgatagctg
    17401 tgttgaagcg cagattctcg atgtcctcgg taactttctt gattgttttg tgccgtagat
    17461 tttctaagtc ttttttggga tttgcttgaa aattggaaat tgaaaattgg aaattccaga
    17521 ttctgtttaa gaatctgtac aggccaacaa tgcttttggt gttccaaggc ttcatgtctt
    17581 ccaaggggcc caaaaacatt tcaaacatac gcagggtgtc tgccccgtat tcttcaataa
    17641 tgtcgtctgg gtttaccacg tttcccttgg attttgacat tttctgcccg ttttcggcta
    17701 aaattatgcc ttgatggcgc aatttccaga agggctctac aaaatctaca tacccaagtt
    17761 tacgcagcac tttggtaaag aaacgggcgt acattaagtg caacaccgtg tgctctgccc
    17821 cgcccatgta tacatcaacc ggcaaccact gctgaattgc tttttttgaa gcaaactctt
    17881 ttccattggc cggatcggca aacctaaaat aataccagga agagcacaca aacgtgtcca
    17941 tagtatctac ttccggtttc cagcctttgc cgaagatttt ttctgtgcgc cttacaaact
    18001 cttttgaatc tgccaaaggc gaatacccgg taggtttaaa ctccgcgtct gtcggcagca
    18061 tccaaggcaa atatttttct ggcaccgcat gcggctttcc ctcggggtcg tacacaatag
    18121 gaataggggc gccccaatac cgctgcctgg aaaccagcca atcgcgcaaa tgatagtttg
    18181 ttttctttgt gccgcctacc gctttggtaa tttccccttt tgctttttca ttgggcatgc
    18241 catcgaactt ccccgaagcc actaaatggc cttctccaag atacgcctcg tccatcacag
    18301 gacaagtttt ggcagggtaa tgcgggcata ccaccattat aatcggcaag ttgtattttt
    18361 gggcaaactc aaaatcgcgt tcatcgtgcg cgggaactgc cattatggcg ccagttccgt
    18421 agcttgccag cacataatct gcaatccata taggaatttc ttctttggtg cctggattta
    18481 ttgcttttac tccttttagc tccacgcctg ttttttcttt ggaaacttct gtgcgctcga
    18541 gttcgttctt tttt
//
LOCUS       DASRUP010000066         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_337599, whole genome shotgun sequence.
ACCESSION   DASRUP010000066 DASRUP010000000
VERSION     DASRUP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337599"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFE94_02610"
     CDS             complement(<1..300)
                     /locus_tag="VFE94_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZX50022.1"
                     /translation="MEDCIFCKIAERKAPAEIVHEDEHFMVFPDINPKASLHLLIIPK
                     KHIENLQEVPESDTELMGKLLLCAQKVANKKQLKGYKLAMHVGREGGQEVDHLHMH"
     gene            complement(302..1609)
                     /gene="hisS"
                     /locus_tag="VFE94_02615"
     CDS             complement(302..1609)
                     /gene="hisS"
                     /locus_tag="VFE94_02615"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZX50023.1"
                     /translation="MSKKLKFQTPTGMHDVLPEDQFLYEKIYGVVDDVAKFYGFGKIE
                     TPMVEDAALYEKSTGATTDIVEKQMYAFKTKGGDALALRPEFTPGIVRSYIQHGMINL
                     PQPVKLYSYGPLFRYEHPQAGRFRQFRQLDFEVFGEQSAAIDAQIIQIFYAILKDLKF
                     SHLIIEVNSIGDNQCRPYYKKLLSNYLRPRQNSLCADCRRRIRENPLRTLDCKEEKCQ
                     RVRSQAPQIIDHLCEECKTHFKSLLEFLDEADLPYHLDPYLVRGLDYYTKTVFEVFSD
                     KVMEGTSVEGEVVQRSALVGGGRYDALARMLGGKDVPASGAAAGVERILSLMKEMQLA
                     GVPEQPVKVFLAQLGDLPKKKSLKLMEEFRKARIPIAESLGRDSLRTQLARADKLEVE
                     YTLILGQREVLDGTIVIRKMDTGEQQNVKSENVVEEIKKRLKK"
     gene            complement(1606..2157)
                     /gene="lepB"
                     /locus_tag="VFE94_02620"
     CDS             complement(1606..2157)
                     /gene="lepB"
                     /locus_tag="VFE94_02620"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZX50024.1"
                     /translation="MKSTLSFLWEIAKIAVIALVLVVGMRLFVFQPFLVRGASMEPNF
                     HNWDYLIVDELSYRFREPQRGEVIVFKFPNNPADRYIKRIIALPGETVVVEAGKVGVY
                     RGQEEVILDEREYLSLLDTPGSVQTTLGPNEYFVMGDNRPESSDSRSWGTLSEDLIIG
                     RVLLRVFPISAFAKVEAPEYESL"
     gene            2218..2928
                     /locus_tag="VFE94_02625"
     CDS             2218..2928
                     /locus_tag="VFE94_02625"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZX50025.1"
                     /translation="MYLIAGLGNPGTQYAKTRHNAGFLVVDTFAKEFGFPAYSLDQKA
                     NALVAENILSHMKVMLVKPQTMMNASGKAIRVLVQRHGIRDWLLTPSGSAKLQQRHSQ
                     SARGILNTPVAFLKRQTGIMQALSPMRAEREPSYLIVVHDDLDLALGKIRISVGSGSA
                     GHRGVESVIQQLGNKDFARIRLGILPKEKPSDVEDFVLKRFMPEEEPIAQQAVLNAVH
                     ALELIVTDGIERAMNEYN"
     gene            complement(2969..>3519)
                     /locus_tag="VFE94_02630"
     CDS             complement(2969..>3519)
                     /locus_tag="VFE94_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX50026.1"
                     /translation="SDLADANLEEVIRGIRSSQLGIIVLAEKTQGDNVRLAVAFAAGA
                     DDYIRLPVDLSELMLRTYAVLRRLQAPEVAGVSTKKSSLRVGDLLLKSGTQELFLAGK
                     KIELTSTEFEMLYLLVSNRHRVVSKGTLKSEVWGANYRDEKIVQKYVQRLRQKLGDDA
                     KNPILIATVHGVGYRFIGPPPE"
BASE COUNT          872 a          925 c          783 g          939 t
ORIGIN      
        1 gtgcatatga agatggtcta cttcctggcc cccttctctg cctacgtgca ttgcaagttt
       61 atatcctttt agttgttttt tgttggcgac cttttgagcg caaagtaaca atttgcccat
      121 cagttctgta tcactttctg gcacttcttg caagttttca atatgctttt tgggaataat
      181 cagcaagtgc aaggaagcct tagggttaat atcgggaaac accataaagt gctcgtcctc
      241 gtggactatt tcggcgggcg ctttcctttc tgctattttg cagaaaatac agtcttccat
      301 gttatttctt gagccgcttt tttatctctt ccaccacgtt ttctgacttc acgttttgct
      361 gttctcccgt atccattttt cgaattacaa tggtgccgtc cagcacttcg cgctggccca
      421 agataagcgt gtactccacc tccagcttat cggctcttgc cagttgcgtg cgcaaagaat
      481 ccctccccaa agactctgca ataggaattc tggcttttcg gaattcttcc ataagcttta
      541 agctcttttt cttgggcaag tcgccaagct gcgcaagaaa cacttttact ggctgctctg
      601 ggacgccggc aagctgcatt tctttcataa ggctcaaaat gcgctctact ccggcagcag
      661 ctccggaagc aggcacgtct ttccctccga gcatgcgggc caaagcgtcg taccttcccc
      721 ctcccaccaa agcgctgcgt tgtacaactt cgccttccac agaagttccc tccataactt
      781 tgtctgaaaa cacttcaaat acggtctttg tgtagtagtc gagtccgcgc actaaataag
      841 ggtctaagtg atatggcaag tcggcttcgt ccaagaactc cagtagcgac ttaaagtgcg
      901 ttttgcactc ttcgcacaag tgatcgataa tctggggagc ctgcgatcgc acccgctggc
      961 acttttcttc tttgcagtct aacgtacgca aaggattttc gcggattctt cgcctgcaat
     1021 cggcgcacaa agagttttgc cgcgggcgca agtagttaga gagcagtttc ttgtaataag
     1081 ggcggcactg gttgtcgcca atgctgttta cttcgattat caaatgggaa aattttaaat
     1141 ctttgagtat ggcgtaaaaa atttggatta tctgggcgtc tattgccgcc gattgctctc
     1201 cgaatacctc aaaatccagt tggcggaact ggcgaaaacg gccagcttga ggatgttcgt
     1261 atcgaaacaa aggcccgtag gagtaaagct tcacgggctg gggcagattt atcatgccgt
     1321 gctgaatgta ggatctcacg atgcctgggg taaattcggg gcgtaaggcc aaagcatcgc
     1381 cccctttggt tttaaacgcg tacatttgct tttcgacaat gtcggtagtg gcacccgtac
     1441 ttttctcgta caaagcggcg tcttccacca tgggcgtctc aatttttcca aaaccgtaaa
     1501 acttggcaac gtcgtccact accccataga ttttttcata caaaaactgg tcttctggca
     1561 agacatcgtg cattcctgta ggagtctgaa atttgagttt tttgctcata gcgattcgta
     1621 ctctggcgcc tccactttcg caaacgccga aatcgggaat acccgcagca atacgcgccc
     1681 aataatcaag tcttcagaga gtgttcccca acttcgagaa tcagacgatt ccggcctgtt
     1741 gtctcccatt acgaaatatt cgtttggccc aagagtcgtc tgtaccgatc ccggagtatc
     1801 gagcaaagaa agatattccc tttcgtcgag gataacctct tcttgcccgc gatacacgcc
     1861 cacctttcct gcttccacga ctacggtttc tcccggcagg gcgataattc gcttaatgta
     1921 cctgtcggca ggattgttgg ggaatttaaa aacaataact tcgccgcgct gtggctcccg
     1981 gaaccgatac gaaagctcgt cgacaatgag ataatcccaa ttgtgaaagt taggctccat
     2041 agaggctccc cttactaaaa agggctggaa cacaaacagg cgcatgccta ccaccaatac
     2101 caacgcaata acggcaattt tggcgatttc ccaaagaaaa gaaagggtgc ttttcatatt
     2161 actcccctat tatacccaaa tatgttcttt tttgcaagat tcggttatac tatctcaatg
     2221 tatcttatag ccggcctcgg caacccagga acccaatacg cgaaaactcg ccacaacgca
     2281 gggtttttgg tggtggacac ctttgccaaa gagtttggat tccctgcata tagtctcgac
     2341 caaaaggcaa acgcgcttgt tgcagaaaac attcttagcc acatgaaagt gatgctggtc
     2401 aaaccacaga ccatgatgaa tgcctctggc aaagccatac gagttctcgt acagcgccac
     2461 ggcataagag actggctgct cacgccttcg ggatctgcaa agctccaaca gcggcactcc
     2521 caaagcgccc gaggaatact caacactcct gtcgcctttc tcaaaaggca aacagggatt
     2581 atgcaggctt tgtcgcccat gcgggcagag cgcgaaccca gctatcttat tgtggttcac
     2641 gacgaccttg atttggctct ggggaaaatc cgcatttccg tggggtcggg atctgccggg
     2701 caccggggag tggaatctgt tatacaacaa ttgggcaaca aagattttgc cagaatacgc
     2761 ctgggcattt tgcccaaaga aaaaccttcc gatgtggaag attttgtact caagcgcttt
     2821 atgcccgaag aagaacctat tgcgcagcaa gcagttctca acgctgtgca cgccctcgaa
     2881 ctcatcgtta ctgacggcat agaaagggca atgaacgagt acaactaaaa gaaaaagtcg
     2941 gcggaaccat ggttccgccg actggttttt attcgggtgg tggaccaata aatcggtagc
     3001 ctactccatg caccgtagcg atcaatattg gattcttggc atcgtctcct agtttttgcc
     3061 gcagtcgttg gacatatttt tggactatct tctcgtcacg atagttcgcc ccccacactt
     3121 cacttttcaa ggttccctta gaaactaccc tgtgcctatt gctcactaaa agataaagca
     3181 tctcaaactc cgtagacgtc aactctatct ttttccctgc caggaaaagc tcttgtgttc
     3241 ctgacttaag caacaagtcg ccgacccgca aagaagactt ctttgttgac acacctgcaa
     3301 cctctggcgc ctgaagccta cggagtaccg cgtatgttcg aagcatcaat tccgatagat
     3361 ctacaggtaa acgaatgtag tcatccgccc ctgcggcaaa agcaacagct aagcgcacat
     3421 tgtctccctg tgttttctcg gcaagaacga tgatgccaag ttgtgaggaa cgaattccac
     3481 ggattacctc ttcgaggttt gcatccgcca aatccgagc
//
LOCUS       DASRUP010000067         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1081084, whole genome shotgun sequence.
ACCESSION   DASRUP010000067 DASRUP010000000
VERSION     DASRUP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081084"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFE94_02635"
     CDS             <1..403
                     /locus_tag="VFE94_02635"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZX50027.1"
                     /translation="SQIRLNAKLNGINSKRYRVLQSDVFSGVKGKYDYILANPPYVPE
                     SRKSRVQKSVLEQEPYHAVFGGKDGLQYIREFLGEAEKHVVPGGVVYLEFDSLQKVEV
                     VRLAKEVGAASVEPCKDQYNKMRYARIVFTA"
     gene            378..1601
                     /gene="xseA"
                     /locus_tag="VFE94_02640"
     CDS             378..1601
                     /gene="xseA"
                     /locus_tag="VFE94_02640"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZX50028.1"
                     /translation="MQESFLQPEMDREGGMIYSVEECHFLVQETLDRLGRVKVRGEMN
                     DVKTVRGQMAFFDLKDSNGKDFVVKCSIFGWEFKKYLHLIENGMEVVAVGKLRMYKNG
                     SLSIVVQEVQPYGEGAWRKAFEALKKKLEAKGYFDESRKREIPQVIQRIGLITSEEAQ
                     GLYDFRQNLGNYGFKVFLYSVWVEGDRAEESIVGAFQWFNKNRPDLDVLVLIRGGGGF
                     ENLKVFNSEKIADAIASSRLPVITGIGHEKDESIADYVADKRLSTPTAVAAFLRTQRE
                     ELIRDVEAIRESLQEKMQDVLEEKKHAINTQAERLHRGFGRVFEGFATLKQRLLHAHY
                     TYEKQIELYFARVERIQGMLASLNPENVLQRGYSVVFNAEKKVVKDASQLKLGEQVGV
                     RVAKGRFVSRVEEIQ"
     gene            1608..1826
                     /gene="xseB"
                     /locus_tag="VFE94_02645"
     CDS             1608..1826
                     /gene="xseB"
                     /locus_tag="VFE94_02645"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZX50029.1"
                     /translation="MAQQKPNLQEALQKLEQLVKDLEQKDVDVEAGLAKFKEGVVLVK
                     FCQGQLKEAENEFKKLKTELEQAEEALE"
     gene            complement(1786..>2032)
                     /locus_tag="VFE94_02650"
     CDS             complement(1786..>2032)
                     /locus_tag="VFE94_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HZX50030.1"
                     /translation="MPPRHVTIHELELLEFKPPFARLRVVCSSGTYIRTLVEDIGKAL
                     GVGAYTKELKRTRVGEYKIEDSKTLIPTPLLLARVLF"
BASE COUNT          613 a          401 c          572 g          446 t
ORIGIN      
        1 ttcgcaaatt cgcttgaatg cgaaactcaa tggtattaac tcaaagcggt atcgagtttt
       61 gcaatccgac gttttctcag gggtgaaagg aaaatacgat tatattctgg caaatccgcc
      121 ctacgtgcca gaaagcagga aatcacgcgt gcaaaagtcc gtgttggagc aggaacccta
      181 ccatgctgtt ttcggcggca aagatgggtt acaatatata cgagagtttt taggcgaagc
      241 agaaaagcat gttgtccccg gcggggtagt gtatctggaa ttcgactcgc tccagaaagt
      301 agaggtggtg aggttggcaa aagaagtcgg agccgcaagt gtagaaccat gtaaagacca
      361 atacaataag atgcgctatg caagaatcgt ttttacagcc tgaaatggac agggagggcg
      421 gcatgatata ctccgtggag gagtgccatt ttttggtgca ggaaacactc gatcgtttgg
      481 gcagggtaaa ggtgcgaggc gaaatgaacg acgttaaaac cgtgcggggc caaatggcct
      541 ttttcgacct caaagactca aacggcaaag actttgtggt aaagtgctcg atattcggct
      601 gggaatttaa aaagtacctg caccttattg aaaacggcat ggaggttgtg gcggtgggga
      661 aactgcgcat gtataaaaac ggttctttga gcattgtggt gcaggaagtt cagccgtatg
      721 gcgaaggcgc gtggcgcaag gcattcgagg cattaaaaaa gaaactcgag gccaaagggt
      781 atttcgacga atcccgaaaa cgcgaaattc cgcaggttat ccagcgaatc gggctgatta
      841 cttcagaaga agcgcagggc ttgtacgact ttcgccagaa cctggggaac tatggcttta
      901 aggtattttt gtacagcgta tgggtagagg gcgatcgagc cgaagaaagc atagtgggag
      961 cattccaatg gtttaataaa aacaggcccg atttggacgt attggtgctt attcggggcg
     1021 ggggcgggtt tgaaaacctc aaagtgttta attcagaaaa aattgccgac gccattgctt
     1081 cttcccggtt gcccgtaata accggcattg gccacgaaaa agacgaaagc atcgcagact
     1141 atgtggcaga taaacggctt tcgactccta ctgcggttgc cgcatttttg cgcacccaaa
     1201 gagaagagct catacgggat gtagaagcga tacgggaaag tttacaagaa aaaatgcaag
     1261 acgtattgga agagaaaaag cacgcgataa acacgcaggc agagcgcttg caccgcggct
     1321 ttggcagagt gtttgaaggc ttcgccaccc tcaagcagcg gttgttgcac gcgcattata
     1381 catacgaaaa acaaatagag ctgtactttg caagggtgga gcggattcaa ggcatgcttg
     1441 cttctttgaa tccggaaaat gtgctacaac gagggtacag cgtggtgttt aacgcagaaa
     1501 agaaagtggt aaaagatgca tcgcagttga agctgggcga acaggtgggg gttcgcgtgg
     1561 cgaaaggcag atttgtctcg cgcgtagaag aaatacaata aaaatatatg gcgcaacaaa
     1621 aaccaaatct tcaagaagca ttgcaaaaat tagagcagtt ggtaaaagac ctggagcaaa
     1681 aagatgtcga cgtggaggct ggcttggcaa agtttaaaga gggagtggtg ctggtaaaat
     1741 tttgccaggg ccagctcaaa gaagccgaaa acgaatttaa gaaactcaaa acagaactcg
     1801 agcaagcaga agaggcgttg gaataagggt tttactatcc tcaattttgt actcgccaac
     1861 gcgcgtgcgt ttgagctctt tggtataggc gccaacacca agcgctttgc caatatcttc
     1921 caccagagta cggatatacg tgccagaaga acaaacaaca cggagccgtg caaacggcgg
     1981 cttaaactca agcagttcta attcgtgtat ggttacgtgg cgcggaggca ta
//
LOCUS       DASRUP010000068         9288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1598235, whole genome shotgun sequence.
ACCESSION   DASRUP010000068 DASRUP010000000
VERSION     DASRUP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598235"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFE94_02655"
     CDS             <1..202
                     /locus_tag="VFE94_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZX50031.1"
                     /translation="IQGYLPEQMAEEEIRKLVKEAIAKTGAQSPQDMGKVMAALMPQT
                     KGKADGGQVSQIVKQQLAGGES"
     gene            complement(230..1177)
                     /locus_tag="VFE94_02660"
     CDS             complement(230..1177)
                     /locus_tag="VFE94_02660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZX50032.1"
                     /translation="MIFRASIVAFAFLIPAVSWGFLQWADQEPETVTIPQAVPAAPKV
                     KEPQELTLLFVGDIMLDRGVEYELKKNGADWTWPFHHIADFLAKADLVFGNLESQISD
                     KGVNVGSIYSFRADPQSMQALTYAGFDVLSVANNHSFDYTKQAFEDSKARLQQAGIMP
                     LADELVIKELEHATIGFLAYSNFPGPALVDWNNLNQVIRNIRQSAEKVDILVVSLHAG
                     EEYQPQPNQFQKAFARAAIDAGADIIAGHHPHVLQPLEQYKEGWIAYSLGNFVFDQKF
                     SEETMRGAILKVVIADKKIKEASLLPTKLNDSYQIELVQ"
     gene            1238..1654
                     /gene="ybeY"
                     /locus_tag="VFE94_02665"
     CDS             1238..1654
                     /gene="ybeY"
                     /locus_tag="VFE94_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZX50033.1"
                     /translation="MIEVSNRTRVQIDENALRKTAESVLRKEGKRGVGLSIALVGPIE
                     AKALNKKHRKKSNVPNVLSFSLGKKMPGLNEAELGEIVLCPLQIRKDAQKYGILFDKQ
                     LTWMLIHGVLHLLGYDHATDKEARAMEQKEQDYLFS"
     gene            1651..2856
                     /gene="ftsA"
                     /locus_tag="VFE94_02670"
     CDS             1651..2856
                     /gene="ftsA"
                     /locus_tag="VFE94_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZX50034.1"
                     /translation="MKAKILTGLDIGTHSIKMAVLQKREGGEELEILGFGEELSSGVR
                     RGQVVSPEEVSRHVVALKNRIENISGQHIEEVVCNVGGSHLFMVPSHGIVAVSRADGH
                     ISQEDVERVLQAAQAFSLPSNKEILEVFPRHFVVDGQEQVKEVVGMKGVRLEADVLAV
                     CGFSPYVKNATDVVLISGLEVLDVVPSPLMASSCVLTRQQKELGVGVLDMGAGTTGLA
                     VYEEGDLIHTAVFPIGAENITNDIAIGLRCEPAVAERVKLEFGTCQPGKGRKMERIEL
                     ADGEHLVFSQKILSHIIEARVKEIFQLVNKELKQISRQGTLPGGMVLSGGGARLPKIV
                     ESAKKDLKVTVKVGTPHGVASVQSDPAYLGVIGLAQLAYDEDYRPQKRKLELGFLQNF
                     KKWFKPFIP"
     gene            2860..3795
                     /gene="ftsZ"
                     /locus_tag="VFE94_02675"
     CDS             2860..3795
                     /gene="ftsZ"
                     /locus_tag="VFE94_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZX50035.1"
                     /translation="MEQTTTIKVMGVGGAGGNAIERMMQCKIKGIELIAVNTDMQDLK
                     KIRAHRKIRIGQESTRGLGAGMNPELGKRAAVESREELTEVLKGADMVFVTAGLGGGT
                     GGGAIPVVAEIAKSQGALTIAVVTRPFSFEGIWRGKLAERALLELKSKVDTLLVIPND
                     QILQIIDQDTSVISAFWKADEILREAVQGISDLIVVPGIINVDFADLKSIMKNSGKAV
                     FGMGQAKGEKRIDQALEEALHSPLLDISIKGAKGILFNVGSSDDVTLSEIQEAAKKVQ
                     EQVAPQAKIIFGAVHDKSLKQGEVRITLIATGFTG"
     gene            3792..5120
                     /locus_tag="VFE94_02680"
     CDS             3792..5120
                     /locus_tag="VFE94_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZX50036.1"
                     /translation="MTFSKPFLAACLVLAVLFIWRYQTVETEALEKAGAITQYAGQEV
                     QFEAKVVKAPEERFSSMHAVVQPIFATEGRILLTTQEAFLVQYGDIIQVQGILERPGT
                     FDGFDYAKFLAKDGIYAIMRDPKVEVVQRGAYSRLGERTLAAVFALKETFRDTLEQHL
                     SSRHSSVMVEMLLGDKDVMSDDLAQDLNATGLRHIIAISGAHIAILTMYLMPFLIWFG
                     LWRQQAFYVTLALLIFYVVLTGLQPSAVRAGIMGGMFLLGQHLGRPYAALRALVLSAG
                     AMLLLNPLLLTRDAGFQLSFLAVLGMILLLSPMLSLLPKKMPARQLLAMTLAAQVFTL
                     PILIWNFGQISVVSVVTNILIVPIVPLLMGLGFALMIGGTLLSFVGLLVSFPVAVMLE
                     YTLWVVDFFAAFPFAAVQTENFPWFWFLPFYAVVAFLHWKLRKREGFLVY"
     gene            5127..6407
                     /locus_tag="VFE94_02685"
     CDS             5127..6407
                     /locus_tag="VFE94_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50037.1"
                     /translation="MFGLRRKKAWVVAVDMGYGHQRAALPFKDIAQGGQIITANNYPG
                     IAQGDRKIWEDTQRLYETISRFKKFPILGDFVFSLFDKFQEIKEFYPSTQSIDSPTMQ
                     LKQIYRIMENKKWGRNLIWKLNRKPLPLITTFFTPAYMAEFWGYKGPIYLVIPDTDIS
                     RAWAPLKPSQSKIIYCASTQRAADRLQRYGVREKNVVVTGFPLPGEFTRASAFLAKRD
                     LKRRLVVLDPEGKYRARYEKLVEQYVGKLPSEKAKQPVNLTFAIGGAGAQVEIAKDMA
                     ESVAPLLKSGKLRLHLVAGIHRELAADLRKKLKARSIVIHSSPSKKKYFADFARLLSQ
                     TDILWTKPSELVFYAGLGIPIIIAPPIGSQEVLNRKWLLDIGAGLDQKKPSLVHQWLP
                     DLIREGNFAEAAMQGFVEIEKNGTENIKKLVCGL"
     gene            6395..7315
                     /locus_tag="VFE94_02690"
     CDS             6395..7315
                     /locus_tag="VFE94_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50038.1"
                     /translation="MWVVIAITGYFLLAITNTIDKYLLSGPLLSPKVYSFFVGMLSVA
                     AVLLIPFGVLEFPGAATVLLAIGAGAVRVGALFVLFSGLRKFEVSRIVPALGGAAPIF
                     TLILVFALSGEREVFSGQNFPAFVLLVAGSIGVTLERKALVTVQSLLYAACAALLFSV
                     FFVLAKFVYEAQSFLSGLVWIALGSFLASFVFLGFKEVRDTLHKLRSPKKRRRPAFQK
                     LGVLVAGNQIIGGSAIFLQSLAVALVPFGLLPFVNALEGVTYFFLFVITLFLSLYFPS
                     VLKESSSPWSIAQKVISIILIGAGLALLAA"
     gene            7312..8310
                     /locus_tag="VFE94_02695"
     CDS             7312..8310
                     /locus_tag="VFE94_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HZX50039.1"
                     /translation="MKKFLKVFGIGILLLLAVGAAYLFVGGTQEAKTITWGVNFSANH
                     ATILGLNWQAVYTALLDELQVKRLKVSLDWDSLESERGTFSFANADWLVAEAEQRNAQ
                     LILVVGMKTMRWPECHLPEWAKGLPKEEQQQEILELLEALVARYKDAPALAKWQVENE
                     PLFKFGDCPWVDRKFLEKEVELVKSLDADHPIVMSDSGELSFWIRAARIGDVVSTTMY
                     RKVWFHEIDRYVTYPLPAVFYARKAGYIDTFFGKEVIVGELQAEPWGPGKLLYDTTME
                     EQDRAFDMAQFQKNIEFARQTGMQEFYLWGAEWWYWRKENGNDDSFWTEARKLFIQ"
     gene            8315..>9288
                     /locus_tag="VFE94_02700"
     CDS             8315..>9288
                     /locus_tag="VFE94_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZX50040.1"
                     /translation="MRKPIYLDYAASTPLDKGVLEAMMPYLRRHYGNPSSQHSFGQKA
                     RAAIEQAREQASRFLGCNADEVIFTSGATEANNLVMKGAIQNTKPHIVTTAIEHESVL
                     EPCRSLEKEGIAEVTYVAPQKDGIVRAEDVMGAIKPNTVLVSVMYANSEIGTVQPVGE
                     IGAAIQELRRNKKQAYPVFHSDAVQAANFLDCNTQTLQVDLLTLSSHKIYGPKGVGVL
                     YKRNKMPLKAVLEGGGQEMGVRSGTENVAAIVGMGAALEALHNPRMLVTNIKIRQLRD
                     KLIKGVLRRIPDSKLTGSAERRLANNAHFRFRGVEGRDVVVLLDQKGIA"
BASE COUNT         2390 a         1951 c         2542 g         2405 t
ORIGIN      
        1 tatccaaggc tatttgccag aacaaatggc agaagaagaa atccgcaaat tggtcaaaga
       61 agccattgca aaaaccgggg cgcagagccc gcaagacatg ggaaaagtaa tggcggcttt
      121 aatgccgcaa acaaaaggca aggccgacgg cgggcaggta agccaaatcg taaaacaaca
      181 gctggcagga ggagaaagct agccccgacg gtaccgtcgg ggtttttgtt tactgaacga
      241 gttcaatttg atatgagtcg ttgagtttgg tggggaggag ggaggcctcc tttatttttt
      301 tgtccgcgat tacgactttg agaatggcgc cgcgcatggt ttcttccgaa aacttttggt
      361 cgaatacaaa atttcccaag ctgtaggcaa tccagccttc tttgtattgc tccaaaggct
      421 ggagaacgtg ggggtggtgg cccgcaataa tatctgctcc ggcatcaata gcagcccggg
      481 caaaggcttt ttgaaattgg tttggttgcg gctggtattc ttcgcctgcg tgcaaagaaa
      541 ccacaaggat atcaactttt tctgccgact ggcggatgtt tctgataacc tggttgaggt
      601 tattccaatc aactaaagcc gggccaggga aattgctgta ggcgaggaat cctattgtgg
      661 cgtgctcgag ttctttgata acaagttcgt cggcaagcgg cataatgcct gcttgctgca
      721 gtcttgcttt ggaatcttca aaggcctgtt tggtatagtc aaagctatgg ttgtttgcca
      781 ccgagagtac gtcgaatcct gcataggtta aggcctgcat agattggggg tcggcgcgaa
      841 acgaataaat gcttcctacg tttactccct tgtctgaaat ttggctttcc aggtttccga
      901 atacaaggtc tgctttagcg agaaaatctg cgatatggtg gaaaggccac gtccaatcgg
      961 ccccgttttt cttgagttca tactctacgc cgcggtctaa cataatgtcg cccacaaaga
     1021 gcagcgtaag ctcttggggc tcttttactt ttggtgctgc gggtactgct tgtggtatgg
     1081 ttactgtttc tggttcttgg tctgcccact gcaaaaagcc ccagctcacc gcaggaataa
     1141 gaaaggcaaa cgcaacgata cttgctcgaa aaatcatacc gtgtttatac cttagattta
     1201 cacctttttt tcgacagtgt acaatagaag attgccaatg attgaagttt ccaacagaac
     1261 tcgagtacag atagacgaaa atgcgttgag aaagactgca gaaagcgtat tgcgaaagga
     1321 agggaaacgg ggggttgggc tttcgattgc gcttgtgggc cccatagagg caaaagcgct
     1381 gaacaaaaaa catcgtaaga aaagcaacgt gcccaacgtt ttgtcgtttt ccttgggtaa
     1441 aaaaatgcct ggtttgaacg aagctgagct gggagaaatt gtgctttgcc ctctgcagat
     1501 aaggaaagat gcccaaaaat atggtatact ttttgacaag cagctaactt ggatgcttat
     1561 tcacggtgta ctgcatcttt taggatatga ccatgccacc gacaaagagg cgcgagccat
     1621 ggaacaaaaa gaacaagact accttttttc atgaaagcta aaattttaac cggcctcgat
     1681 attggaacgc actccataaa aatggctgtt ctccaaaaaa gagaaggggg agaggagctt
     1741 gaaattcttg ggtttggaga agagttgtcg tctggagtaa ggagggggca ggtggtaagt
     1801 ccagaagagg tctcgcgcca cgtcgttgcc ttaaagaacc gcatcgaaaa catctcgggc
     1861 cagcatatcg aagaagtggt ctgcaatgtc gggggaagcc atttgtttat ggtgccctct
     1921 cacggaattg tggcggtatc aagagcagac ggccatatct cccaggagga tgtcgagcgg
     1981 gtattgcagg cagcgcaggc tttctctttg ccttcaaaca aggaaattct cgaagtattt
     2041 ccgcgccatt tcgtggttga cggccaagag caggtaaaag aggtggttgg catgaaaggc
     2101 gtaaggctcg aggccgatgt tcttgccgta tgcggatttt ccccttacgt aaaaaatgcg
     2161 acagacgtcg tgcttatctc ggggcttgaa gttctcgatg tcgtcccttc tccgcttatg
     2221 gcgtcttctt gcgtgctcac gcgccagcaa aaagagttgg gggttggagt gctcgatatg
     2281 ggggccggca ctacaggttt ggcggtatac gaagaaggcg atttaattca tacggctgta
     2341 ttccccatag gcgctgaaaa cattaccaac gacattgcca ttggattgcg gtgcgaaccc
     2401 gctgttgcag aacgcgtaaa actcgaattc ggcacatgcc agccgggaaa aggcaggaag
     2461 atggaaagaa tcgagttagc cgacggggag catttggtat tttcacagaa aatattgagc
     2521 catattatcg aagcccgggt caaagaaatt tttcaactgg taaataaaga gttaaagcag
     2581 atttcccgac agggcacgct tccgggagga atggtattga gtggaggagg ggcaaggttg
     2641 ccaaaaatcg tggaatctgc gaaaaaggac ttaaaggtta ccgtaaaggt aggaacaccc
     2701 catggggtgg ccagcgtgca gtccgatccc gcctacttgg gcgtaatcgg cttggcacag
     2761 cttgcctacg acgaagatta tcgcccgcag aaaaggaaac tggaactcgg ctttttgcag
     2821 aattttaaga aatggtttaa accctttatt ccttaggata tggagcaaac aacaacaatt
     2881 aaagtcatgg gagtgggcgg cgcgggggga aacgccatcg agcgcatgat gcagtgtaag
     2941 attaagggca ttgagcttat cgcggtaaat accgacatgc aggatttaaa gaaaatccgc
     3001 gcccatcgga aaatacgcat aggccaggag tcaacgcgcg gtctgggagc cggcatgaat
     3061 cctgaacttg gcaagagggc tgccgtggag agcagggaag agctcacgga agtcttaaaa
     3121 ggagcagaca tggtatttgt aaccgcaggg ctcggaggcg gaacaggggg aggagcaatt
     3181 ccggtggtag ctgaaattgc caagtcccag ggagccctga ccattgcggt ggtcacgagg
     3241 cctttttctt ttgagggcat atggaggggt aagctggcag aacgcgcgct tctggaatta
     3301 aaaagcaagg tagatacctt gttggttatt cccaacgacc agattttgca aattatagat
     3361 caggatacgt cggtaatttc tgccttttgg aaggcagacg agattttgcg ggaagcggta
     3421 cagggcattt ccgatttaat cgtggttccg ggcattatca acgtggactt tgcggactta
     3481 aaaagcatta tgaaaaactc gggcaaagca gtgtttggca tgggccaggc aaagggagaa
     3541 aagcgcatcg accaggcgtt ggaagaagcg ctgcattctc cgctgctcga catttccatt
     3601 aaaggcgcca aaggcatttt gtttaacgta ggcagttcag acgatgtaac cttaagcgaa
     3661 atccaggaag ctgcaaaaaa agtgcaggaa caagtggctc cccaggcaaa aattatattc
     3721 ggcgcagtgc acgacaagag tctcaagcag ggagaagtac gcatcactct tattgccacc
     3781 gggtttacag gatgactttt tccaaaccgt ttcttgctgc gtgccttgtg ctcgcagttt
     3841 tgtttatctg gcgttaccaa accgtagaaa ctgaagcgct tgaaaaagca ggcgcaatta
     3901 cccaatacgc aggccaggag gtgcagtttg aagccaaggt agtaaaagct cccgaagagc
     3961 ggttttcttc tatgcatgcg gtggtgcagc caatatttgc gacagaaggc cgcattctcc
     4021 ttactacgca ggaagctttc cttgtgcagt atggtgatat tatccaagtg caaggaatac
     4081 tggaacgccc cggtacattt gatggttttg actacgcaaa atttctggca aaagatggca
     4141 tatacgccat aatgcgggat cccaaggttg aggttgtgca aagaggcgct tactctcgct
     4201 tgggagaaag gacactggcc gcggtgtttg ctctcaaaga aacatttcga gatactttag
     4261 agcaacatct ttcttcgcgc catagttcag taatggtaga aatgcttttg ggagacaaag
     4321 acgtaatgtc agacgatttg gcgcaggact taaacgccac ggggctgcgg cacattattg
     4381 ccatttccgg agcgcatatt gccatactta ccatgtattt gatgcctttt ttgatatggt
     4441 ttggtttgtg gcgccagcaa gctttttatg tcactctggc tctgttgatt ttctatgtag
     4501 tgcttacggg tttgcagcct tctgcggtaa gagcgggcat aatgggcggg atgtttttgc
     4561 ttggccagca tttgggaagg ccgtatgccg ctttgcgggc gttggtgctg tcggccggcg
     4621 caatgctttt gctcaatcct ttgttgctca caagagacgc aggattccag ctttcgtttc
     4681 tcgcggtgct tggcatgatt ttgctgcttt cacccatgct ttctcttttg ccaaagaaga
     4741 tgcctgcacg ccagctttta gccatgactt tagctgccca ggtgttcacg ttgcccattc
     4801 ttatttggaa cttcgggcag atttcggtgg tttcggtcgt taccaacatt ctcattgttc
     4861 ctatagtgcc tttgctcatg ggtttggggt tcgcgttgat gattgggggg acattgcttt
     4921 cgtttgttgg acttttggtt tctttccccg tggccgtaat gctggaatat actttatggg
     4981 tagtagattt ttttgccgca tttccctttg ccgccgtgca aaccgaaaac tttccgtggt
     5041 tctggttttt gcctttttac gccgtcgtcg ctttcctgca ttggaaactt cgaaagaggg
     5101 agggatttct ggtatactag acaaagatgt ttggattgag aaggaaaaaa gcctgggtgg
     5161 tggcagtgga catggggtat ggccatcaaa gagctgcgct gccatttaaa gatattgcgc
     5221 agggagggca gattattacg gcaaacaatt atcctggaat tgcccaggga gatcgcaaga
     5281 tttgggaaga tacgcagcgc ctatatgaaa ccatttcgcg ttttaaaaaa ttcccgatac
     5341 tgggagattt tgttttttct ttgttcgaca aatttcagga aataaaagag ttttacccca
     5401 gcacccagtc tattgattct cccaccatgc agttaaagca gatttaccgc ataatggaga
     5461 acaagaagtg ggggagaaac ctcatatgga aactcaatcg caagcctttg cccctgatta
     5521 ccacgttttt tacgccagcc tacatggcag aattctgggg atataaaggg ccgatttatt
     5581 tggtaattcc cgacaccgat atttcgcggg cttgggctcc tctgaagcct tcgcaaagca
     5641 agattatcta ttgcgcctct acccagaggg ccgcagaccg cttgcaaagg tatggtgtgc
     5701 gagaaaagaa tgtggtggta acggggtttc ctttgccagg agaatttaca agagcaagcg
     5761 cttttctggc aaaacgcgat ttgaaacggc gtttggttgt tttggatcct gaaggcaaat
     5821 accgtgcgcg ctacgaaaag ctcgtcgaac agtacgtagg caagctgccg agtgaaaagg
     5881 ccaaacagcc agtaaacctt acgtttgcca ttgggggagc gggcgcacaa gtagaaattg
     5941 cgaaagatat ggcagagagc gtggcgcctt tgttaaagag tggaaagttg cgcctgcatc
     6001 tcgttgcggg aatacaccgg gaacttgccg ctgatttgcg aaaaaagctc aaggcaagaa
     6061 gtattgttat ccattcctcg cccagcaaaa agaaatactt tgccgatttt gccagactgt
     6121 tgagccaaac cgatatcttg tggaccaagc cttcagaatt ggtgttttat gccggactcg
     6181 gtattccgat tataatcgcg cctccgatcg gctcgcaaga agtgcttaac aggaaatggc
     6241 tattggatat tggagcaggt ttggaccaga aaaaaccaag cctggttcat caatggttgc
     6301 ctgatttgat ccgtgaggga aattttgccg aggctgccat gcagggattc gtggaaatcg
     6361 aaaaaaacgg aacagaaaac atcaagaaac tggtatgtgg gttgtaatcg ccattaccgg
     6421 gtatttttta ctcgcaatta caaacacgat agacaaatat ctgctttcgg ggcctctctt
     6481 gagcccgaag gtatattcat tttttgttgg aatgctaagc gttgcggcag tcttgctgat
     6541 tccgtttggg gtattggagt ttcccggagc tgccactgtt cttttggcaa tcggagcagg
     6601 ggcagtgcgg gtcggcgcct tgttcgtgtt atttagcggg ctccggaagt ttgaagtttc
     6661 gcggattgtg ccagctttag gaggcgcggc ccctattttt accctcattc tcgtatttgc
     6721 tttatcggga gagcgtgagg tgttttctgg gcaaaatttt cctgcttttg tattgctcgt
     6781 tgcgggaagc atcggggtta ccttagagag aaaagcattg gtaaccgttc aaagtttgct
     6841 ttatgccgct tgcgctgcat tactcttttc ggtatttttc gtgctggcaa agttcgtgta
     6901 cgaagcgcaa agttttctct ctgggttggt gtggatcgct cttggatctt ttctggcctc
     6961 ttttgttttt ctgggcttca aagaggtgcg agatacctta cataagttgc gctcgccaaa
     7021 aaagcgacgt cgcccggcat ttcaaaaatt aggggttttg gtggcgggga accagattat
     7081 agggggaagc gcaattttcc tgcaaagtct tgcggtcgcc cttgttcctt ttggacttct
     7141 tccttttgta aacgcgttgg aaggagtaac ttattttttc ttattcgtta ttacgctctt
     7201 cctttctttg tatttcccct ctgtgctcaa ggaaagcagt tctccctgga gtattgcgca
     7261 aaaagtgatt tctattattc tcatcggagc aggattagct ttactggctg catgaaaaag
     7321 tttttaaagg tttttggcat cggaatactt cttttgctcg ccgttggcgc tgcatactta
     7381 tttgtaggag gtacgcaaga ggccaaaacc attacatggg gcgtgaattt ttctgcaaac
     7441 catgcgacaa tccttggctt gaactggcag gccgtataca ccgccctttt ggacgagttg
     7501 caggtaaagc gcctgaaggt gtcgctcgat tgggatagtt tggagtcaga gcgcggcaca
     7561 ttttcctttg cgaatgcaga ttggcttgtg gcagaggcag aacaaagaaa tgcgcaactt
     7621 atactggttg tcggaatgaa aaccatgcgg tggccagaat gccaccttcc cgaatgggca
     7681 aaagggctgc cgaaagaaga gcagcagcaa gaaattcttg agcttttgga ggccctagtg
     7741 gcgaggtata aagatgcccc ggctttagct aaatggcagg tggaaaacga gccgttgttt
     7801 aagtttggcg attgtccgtg ggtagacagg aagttcttag aaaaagaagt ggagttggtg
     7861 aagtcgcttg acgccgacca ccctatagtg atgtcagata gcggagagtt atcgttttgg
     7921 attcgggccg cgcgcattgg cgacgtggta agcactacca tgtaccgcaa agtttggttt
     7981 cacgaaatcg atcggtatgt gacctaccct ttgcccgccg tcttttacgc aagaaaggca
     8041 gggtatattg atactttttt cggcaaagaa gtaattgttg gggaattgca ggcagaaccc
     8101 tgggggcctg gcaaactgct gtacgacacc actatggaag aacaagacag agcttttgat
     8161 atggcgcaat tccaaaaaaa catcgaattc gcacgccaaa ccggcatgca agaattctac
     8221 ctgtggggag cagaatggtg gtattggaga aaagaaaatg gaaacgatga tagtttctgg
     8281 acggaggctc gaaaattgtt tatacaataa agacatgaga aagccgattt atttggatta
     8341 tgcggcctcg acacctttgg ataaaggggt gttggaggcg atgatgccat atttgagaag
     8401 gcattacggc aacccttctt cgcagcacag ctttgggcaa aaggcacggg ctgccattga
     8461 acaagccaga gaacaggcct ctcggttttt gggttgcaac gcagatgagg taatcttcac
     8521 gagtggtgct acggaggcga ataacttggt aatgaaaggc gccatacaaa atacaaaacc
     8581 gcatattgta acaacggcca tagaacatga atcggttttg gaaccgtgca ggagcctcga
     8641 aaaagaggga attgcagagg ttacctatgt ggcgccccaa aaagacggta ttgtgcgcgc
     8701 agaagatgtt atgggagcaa taaagcccaa cacggtattg gtttctgtta tgtatgcaaa
     8761 cagcgaaatc ggcactgtgc aaccggtagg ggaaatagga gctgcaatcc aggagttgcg
     8821 gcgtaacaaa aaacaggcct accccgtttt tcatagtgac gcggtgcaag ctgcgaattt
     8881 tttagattgc aatacccaaa cgcttcaggt ggatttgctt acgttaagct cgcataaaat
     8941 ctatggtccc aagggagtcg gcgtgctgta taaaagaaat aaaatgccgc tcaaagcagt
     9001 gctggaagga gggggtcagg aaatgggtgt gaggtctggc accgaaaatg ttgctgccat
     9061 tgtaggcatg ggagccgcgc ttgaggccct gcacaatccc cgcatgcttg ttaccaacat
     9121 taaaatccgt cagcttcgag acaagctcat caaaggagta ttgcgaagga ttcccgacag
     9181 caagcttact ggttctgcag aacggcgttt ggcaaacaat gcgcactttc ggtttcgcgg
     9241 ggtagagggc cgcgacgtgg tggtgctgct cgaccaaaaa gggattgc
//
LOCUS       DASRUP010000069         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1912194, whole genome shotgun sequence.
ACCESSION   DASRUP010000069 DASRUP010000000
VERSION     DASRUP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912194"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1009)
                     /locus_tag="VFE94_02705"
     CDS             complement(149..1009)
                     /locus_tag="VFE94_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZX50041.1"
                     /translation="MFIDTHGHVNFNAFREDSSEVLQRTLEGHVWVVMPGSQYSTSKR
                     AVEMAEKYEQGVYAAVGLHPIHIGEQRKVDVLEVQSEEKAEQPWETFETRSEEFNYEE
                     YKKLAQSKKVVAIGEIGLDYYYFPKSKVKREEIKRKQKETLRQQMDLADEMHLPVIIH
                     CRVAHEDLLEFLGERKQVRGVVHSYTGTAEQAQKFMDFGLYFGFNGLILKNVPALPDP
                     KEIIASIPLDRIVLETDSPYLLPPGASKERNEPLFVKYIAAEIAKIKKVSLEKVAEST
                     TNNAKKLFTV"
     gene            complement(2467..2556)
                     /locus_tag="VFE94_02710"
     tRNA            complement(2467..2556)
                     /locus_tag="VFE94_02710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2518..2520),aa:Leu,seq:gag)
     gene            complement(2870..>3420)
                     /locus_tag="VFE94_02715"
     CDS             complement(2870..>3420)
                     /locus_tag="VFE94_02715"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HZX50042.1"
                     /translation="LQARLEALGYSLSRDEISSVFESFKVLADKKREVTDRDLEILMD
                     EEKRESTEPVSYTLDQVHFSSGDHTISTATIRLIGPDGKANDGAATGNGPVDAVCKAI
                     DLVIGCSCKLSNFSVDAVDQGKDSQGAVNIRIENNGRIFSGRGASTDIIVASAKAYLS
                     AVNRMLAADEEATLVPVGTTPD"
BASE COUNT          836 a          919 c          705 g          960 t
ORIGIN      
        1 actttgttgt tagcgaccct tctttttttt ctttgccaca atcgttggac ttccttatgt
       61 attcgaagcc catgttcgaa agagtgtata tccggcgcga ccacgccaga actcttacaa
      121 cttttttata ccattgcaca aagtagtgtc aaactgtgaa taactttttt gcattatttg
      181 tggtactttc tgcaaccttt tcgagcgaaa ctttcttgat tttggcaatt tctgctgcaa
      241 tatactttac aaaaagaggt tcattgcgtt ccttggatgc gccaggcggc aaaagatacg
      301 gcgaatcggt ttccaagaca attctatcga gcggaatgga agctattatc tctttgggat
      361 cgggcagggc tggcacattt tttaatatga gcccgttaaa cccaaaatac aaaccaaaat
      421 ccataaattt ttgcgcctgt tctgcggttc ccgtgtagct atgcaccact cctcgtacct
      481 gctttcgctc gccgaggaat tccagcaaat cctcgtgagc aacccggcag tggataataa
      541 ccggtaaatg catttcatct gccaagtcca tttgctggcg cagagtttct ttttgctttc
      601 gttttatctc ctctcttttc actttgctct tcgggaaata gtaataatcc aaacctattt
      661 ctccgattgc caccactttt ttactttggg caagtttttt atattcctcg tagttaaatt
      721 cctcgctgcg cgtttcaaat gtttcccagg gctgctccgc tttttcttca gactgcacct
      781 ctaatacatc caccttcctt tgctcgccaa tgtgaatcgg gtgcaggcct acggcagcat
      841 acactccttg ctcgtatttt tctgccattt ccacagctct tttggaagta gagtattgag
      901 accccggcat tacgacccaa acgtgtccct ccagcgtacg ttgtagcacc tccgaggaat
      961 cctccctgaa tgcgttgaaa tttacatgcc catgcgtgtc aataaacata accttattct
     1021 aatctcttta tctaaaaaga aaagcctgtc atggcgacag gcctatagga gttcctccaa
     1081 gcgcttcaag gagaagtctc ttaagtcccg gatccttctt tcgtccgaaa agaggtatct
     1141 ggacgcattc atatgtagat ccctgtatcc aataaggaac ttctaactcc tcaacgcagt
     1201 acggtcgaaa gaggatgaat cgagaccctt gaacccgtgc cctttcgggt acggtttctt
     1261 taacgtgaag atacaactct cgctccttta aaagaacgga gaatatctcc tcgagttgct
     1321 ctacgcgacc cctccagtct tgttcaagga atactgcaac cggtttattc gaaagcatat
     1381 cacctcccca aagataaggt actggttctt tttaagagcc gtaccagatt cttccacttg
     1441 ttgtcaatcc ctcttttcca cacttctccg cccactcgca ataaaagagg gcctagtcat
     1501 cagactggcc ccgaacgaaa acgaaagatt ttgcccctcg tatttgagga ggcagcatat
     1561 cttccttaag gtgctgcttg ggaacaactt gcccacagcc ataatagcgg ccgggtttaa
     1621 gcaaatttct tctctcattg cgagaaaggc tcaacaaaac tacacatgag ctgcttgccc
     1681 gatctttatc gtccactaaa agacgggcac tctcaactac actgcttaac tctctcagaa
     1741 cctttgccgc aggtctatcg ctttttaggg caacatattc caaaaccatc ttatattccc
     1801 ttcatacctg tgcgccagct tcccacctgc cacggcttta gcaactcaaa atccttattc
     1861 ggttgtttag gtgttttctt attctgacca gaactcatcg ctcctcctca atactgctct
     1921 tctgcaccga atttgattcg agcgcagtga gcgagattct ggcaaatgcc gctcactagg
     1981 ctcaagtcaa accccttcga cttcgctcag ggtaaaaaaa cgacctcccg tagggaggcc
     2041 gtataagttc aaggtttgtt gttctttatt tctctatata cacaaaggcc tcccttttgg
     2101 tgggtcgaat aataattcca ataatctcaa tgaggccttt tataaaactc atattgctat
     2161 tttatgaaaa taaaaagggc ttgtcaagct tttagcttac aagcccatga gccttgcggc
     2221 cctcaataaa ggtgagaaat ttgcgtcgct tattttattc cttggtacgt cgacgcgacc
     2281 cgcaccatta ggtaagccat aggggccaaa gtcaggccac aacccagatg caaaaggttc
     2341 ctcgcaaccc gcacagtcac tactaccaca agcccgacag gcctgcactc cgtcctctat
     2401 tggttttcca ataccgtctt gcatcatccc ctccttggat ttttatgttt tgttaccaaa
     2461 aagtgctggt gcggcaggtg ggagttgaac ccacaagcat ccgtggatgg cacccccctc
     2521 aagagtgtgc cccatgccac gtgggcgaac tgccgcatat gtgctgtttt gagcgcagcg
     2581 agcagaattc aactactcaa actcttctca ctgcgctcaa acaaaacgac ctcctttagg
     2641 gaggccgctc aaagttcttt gctatatctt tcctatttct ttcttagcat aaaaaggcct
     2701 ccctttaacg aggtaacgac aatgaccaag actgccaaag ctcgttgtga gaaacctttt
     2761 atgctcatcg ttcatatctt acgaaaataa aataggactg tcaagagttg cataaatgct
     2821 aacttaacag tcctttacgg aagcgaacca aatcgcctgt gtaactatat tagtcgggag
     2881 ttgttccgac tggcaccaaa gtggcctctt cgtcggcggc cagcatacgg tttaccgcgc
     2941 taagataggc cttggcgctg gccacgatga tgtcggtgga ggccccccgg cccgagaaga
     3001 tgcggccgtt gttctcgatg cgaatgttta ccgcgccttg ggaatctttg ccctgatcaa
     3061 cagcgtcaac gctgaagttg gaaagcttac aactacaacc aataaccaag tcgattgcct
     3121 tacatacggc atccaccgga ccgttacctg tggcagcacc gtcattcgct ttcccatcgg
     3181 gaccgataag acgtatggta gcagtagaaa tggtgtggtc gccgctggag aagtgcacct
     3241 ggtccagggt gtagctcacc ggctcggtgg actcccgctt ctcttcatcc atgaggattt
     3301 ccaggtcccg gtcggtaact tcccgcttct tgtccgccag taccttgaag gactcaaaca
     3361 cgctggagat ttcgtcccgg gacagactgt agcccagggc ctccaggcgg gcctgaagcc
//
LOCUS       DASRUP010000070         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_959408, whole genome shotgun sequence.
ACCESSION   DASRUP010000070 DASRUP010000000
VERSION     DASRUP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959408"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFE94_02720"
     CDS             <1..1227
                     /locus_tag="VFE94_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZX50043.1"
                     /translation="QTIQAVRPEWVLEIEGTVQERPEKLKNPNIATGDIEIAAETVHV
                     LTQAKTLPLPIDTDGYEIGEDTRLHYRYLDLRRERLQHNLKLRHQGIQFIRNFLSNKG
                     FVEIETPVLSKSTPEGARDYLIPSRLYAGKFYALPQSPQQYKQLLQVAGFERYFQIVR
                     CFRDEDPRADRQPEFTQLDIEMSFVEQEDVLQLIETLYTQLVEELFPEKRIQKTPFPR
                     ISYKEAMEKWGSDKPDLRENKEDPNELAFAFIVDFPAFEWKEQENRWDAVHHPFTRPQ
                     VNSIEEIKKDPGGVLAHQYDLALNGYEVAGGSLRIYDPKLLADIFEFMGNSKEDVRAK
                     FGHMLEAFEYGVPPHGGIASGLDRLFAVLLGEQSIREVMAFPKTGDAQDLMMQAPSEV
                     SEQQLKELHLKVEKGK"
     gene            1224..2213
                     /locus_tag="VFE94_02725"
     CDS             1224..2213
                     /locus_tag="VFE94_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZX50044.1"
                     /translation="MISKNLKVFLISFCLSLPFWWGTNTLEDVLTRFFFLQELQTNPE
                     WQAAYAAQIKIEAMLHGAYPLSTKQAQSLSVDAHAVLSTLVKEDGSTKILFEKNGQAP
                     LPIASLTKLMTGLVALKNYSPEQDVFITEQAVGELGETGMLKAGDVFPVKDLLHLALM
                     ESSNDAAAALAQPVGRTEFVAAMNHEASALGLGNTSFVNPTGLDSIAPRGNYSTATEL
                     TQLSVYLSREYPQVFDILSQQRLNLYTADGRFHHAMTNTNVLLDWRDWPAKILGGKTG
                     LTPQAKQCLLLVLESPDKKGYIINVILGSDDRFGEMEKLLRWTLNSYQWNLPQ"
     gene            2195..3307
                     /locus_tag="VFE94_02730"
     CDS             2195..3307
                     /locus_tag="VFE94_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50045.1"
                     /translation="MEPASIDLSQSVIKIATLGTASFLLAFFLTPFLTNILYKHKLWR
                     KTVRKQALGGGGLPFFEKFHSEGEVKTPRFGGVLIWIVPPIIAFVFFVLSYTNIWWFE
                     KFSFLSRGQTWLPLATLVAASAVGFLDDLMQVIAPPTKGFFKPVWSLFGKYVAGGLDL
                     KYRFALVTLIGLTGALWFFYKLEWSTIHVPGNGDWTIGLWYIPLFVIVMLATYSGGVI
                     DGIDGLSGGTFASIFAAFGVIAFAQQQIDLAAFCFVVAGAILAFLWFNIPPARFYMGE
                     TGIMGLTAALTVVAFLTDSVLVLPIIAILLVVESASVIIQLLSKKLRHGKKVFLAAPI
                     HHHFEAKGWPPYKVTMRFWVIGVVAAITGVAIRLLG"
     gene            complement(3591..3815)
                     /locus_tag="VFE94_02735"
     CDS             complement(3591..3815)
                     /locus_tag="VFE94_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50046.1"
                     /translation="MPQFPPFFKVDFAQKESRLGLSETVLGILGRALTYGDWKALRGY
                     KQHGFAYSLRKYPQEVLWIARKDKMGNSAI"
BASE COUNT         1010 a          986 c         1004 g          999 t
ORIGIN      
        1 cagaccattc aggcagtgcg gccagaatgg gtgcttgaaa ttgaaggcac ggtgcaggaa
       61 cgcccagaaa agcttaaaaa ccccaacatt gccacgggcg acattgagat tgcggccgag
      121 accgtgcatg ttctcacaca ggcaaaaacc ctgccccttc ccatagacac cgacggctac
      181 gaaataggcg aggatacgcg cttgcactat cggtacctcg acctgcgccg cgagcgcctg
      241 cagcacaatt taaaacttcg ccaccagggg attcagttta tacgaaactt tcttagcaat
      301 aaagggtttg tcgaaataga aactcccgtg ttgtcgaagt ctacgccaga gggagcgcgc
      361 gactatttga taccctccag gctgtacgca ggtaaatttt acgctttgcc tcaaagcccg
      421 caacagtaca agcaattgct gcaagtggct ggctttgaac ggtatttcca aatcgtgcgc
      481 tgttttcgcg acgaagaccc aagggcagac cgccagcccg aattcaccca gctggatatt
      541 gaaatgtcgt ttgtggagca agaagacgtg ctacagctca ttgaaacact atacacgcaa
      601 ctggtggaag aacttttccc tgaaaagcgc atccagaaaa ctcctttccc ccggatatca
      661 tacaaagagg caatggagaa gtgggggagc gataaaccgg atcttaggga aaacaaagaa
      721 gaccccaatg aactggcttt tgcttttatt gttgactttc cggcgtttga gtggaaagaa
      781 caggaaaatc gctgggacgc ggtgcaccac ccttttacca ggccgcaggt aaattcgatt
      841 gaagagatca aaaaagaccc gggcggcgtg cttgcccacc aatacgacct ggccctcaac
      901 ggatacgaag tggcgggggg aagcctgcgc atatacgacc cgaaattgct ggcagacatt
      961 tttgaattta tgggaaacag taaagaagat gttcgggcaa aattcggcca catgctggag
     1021 gcttttgaat acggcgtgcc gccccacggc ggaattgcct cgggcctcga ccgcttgttt
     1081 gcggtgcttt tgggagaaca gagcattcgg gaggtaatgg catttcccaa aacaggggat
     1141 gctcaagacc ttatgatgca agcacccagc gaagtttcag agcaacagct caaagaactg
     1201 catctcaaag tagagaaagg caaatgattt caaagaatct caaagtattt cttatctctt
     1261 tttgtttgag ccttcctttc tggtggggga cgaatacttt ggaagacgta ctcacccggt
     1321 tctttttctt acaggaactg caaaccaacc ccgagtggca ggcagcatac gctgcccaga
     1381 taaaaattga agcaatgctc cacggagcat atccgctttc tacaaaacag gcgcaatccc
     1441 tttctgtaga cgcacatgcc gtactctcta cgcttgtgaa ggaagacgga agcaccaaaa
     1501 ttctttttga gaaaaacggc caggcacctt tgcccattgc cagcctcacc aaactcatga
     1561 cggggcttgt agcgctcaaa aactattccc cggagcagga tgtgtttatt accgaacagg
     1621 ctgttgggga actgggagaa acaggcatgc tcaaagcagg cgatgtgttt ccggtaaaag
     1681 atctgctgca cctggcgctt atggagtcgt cgaacgacgc tgccgcagct ttagcccaac
     1741 ccgtgggaag aaccgaattt gttgccgcaa tgaaccacga ggcttctgcc ttagggcttg
     1801 gcaatacgtc gtttgtaaac cccaccggat tagactctat tgcccctcgc ggaaactatt
     1861 ctacagcgac agagcttacc caactttctg tttatttgtc gcgcgagtac ccccaagtat
     1921 tcgacatcct ttcacagcaa cggctcaatc tgtatactgc agacggccgc tttcatcatg
     1981 ccatgaccaa tacaaatgtt cttttggatt ggcgagactg gcccgcaaaa attttggggg
     2041 gcaaaacagg acttaccccg caggccaaac aatgcctttt gctggtactc gaaagtccgg
     2101 ataaaaaagg gtatattata aatgttatct taggttcaga cgacaggttc ggcgaaatgg
     2161 aaaaactcct ccgctggacc ttaaactcct accaatggaa cctgcctcaa tagatctttc
     2221 gcaaagcgtt attaaaattg caacactggg aaccgcaagt tttttgctgg cgtttttctt
     2281 aacgccgttt cttaccaaca tcttgtacaa acacaaactc tggcgcaaaa ccgtgcgaaa
     2341 gcaggccttg ggcggggggg gattgccgtt tttcgaaaag ttccattcgg aaggcgaagt
     2401 aaaaactccc cggtttggcg gcgttctcat ttggattgtg ccgcccatta ttgcctttgt
     2461 attctttgtg ctctcgtata ccaacatctg gtggtttgaa aagttcagct ttttgtcccg
     2521 cgggcaaaca tggctgcctt tggcaacact ggtggcggca agcgccgttg gctttttaga
     2581 cgacttgatg caggtaatag ctccaccaac caaaggcttt tttaaacccg tgtggagttt
     2641 gtttggcaaa tacgtggcag gcggcctcga cctgaaatat cggtttgccc tggttactct
     2701 tattggcctt acgggagctc tgtggttttt ttacaagtta gagtggagta ctattcatgt
     2761 tcccggaaac ggcgactgga ctattggcct ctggtacatt cccctgtttg ttatcgttat
     2821 gctggccacg tatagcgggg gagtcataga cggcatcgac ggcctttcag gcggcacatt
     2881 tgcttcgatt ttcgcggcgt tcggcgttat tgcgtttgcc cagcagcaaa ttgacctggc
     2941 cgcgttttgc tttgtggtgg ccggagccat actggctttt ctttggttta atattcctcc
     3001 tgcccgtttt tacatgggag aaaccggaat aatgggccta accgcggctc tcaccgtggt
     3061 tgcttttctt accgactcgg tgctcgtact tcctattatc gctattttac tggtggtaga
     3121 atctgcctcg gtcataatcc agttgctttc caaaaagctg cgccatggca aaaaagtgtt
     3181 tttggccgcc cccattcacc accattttga ggccaagggc tggcctccgt ataaagttac
     3241 catgcgcttc tgggtgattg gtgttgtcgc cgccataacc ggtgtggcca tacgcctttt
     3301 gggatagtgt tacccttgac aagaccataa aaatagcgta tcttaatacc agcccagtta
     3361 acaatgggga aactaataac gacgggaggt tgtgcatgga agccgcgggt gctgtgttgc
     3421 ggagagacca cgtttggtgg gtgcaggagg tgtcttcgcc tccaaccgag gagatacagg
     3481 gagaaaaatg ggccgctgtt gtatggccat ggggtgagag gaccatactt ttccccaacc
     3541 ctctccttgc ggacactatt ttcgactctc tagccgaaag tcgacaaact ttagatcgcg
     3601 ctgtttccca ttttatcttt ccttgcgatc cacaagacct cttgtggata cttgcggagg
     3661 ctgtaagcaa aaccatgctg cttataaccc ctcaaggctt tccaatcccc ataggttaat
     3721 gctcttccga gaatgcctaa aaccgtctca ctcaaaccga gacggctttc tttttgcgca
     3781 aaatcgacct tgaaaaaagg gggaaattgt ggcattgtag gggatatgaa agcaagggag
     3841 ttgcggaaaa agtatccgac atttacttat aaaggatact catacgaggt gaaagacggc
     3901 gacttggaaa tggcgtttga ctttaggctg ggccaaacca ggtttagtcc acgggtggtt
     3961 atcaaaaacg ttccggcgaa aagctggttg cttagcccg
//
LOCUS       DASRUP010000071         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1457897, whole genome shotgun sequence.
ACCESSION   DASRUP010000071 DASRUP010000000
VERSION     DASRUP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457897"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1087)
                     /locus_tag="VFE94_02740"
     CDS             complement(176..1087)
                     /locus_tag="VFE94_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZX50047.1"
                     /translation="MFKRQILGGIVAFSLLAGSIIPSASFAQGTTDTALIQQLQAQIQ
                     SLLAQVQQLTSQISQLQGQQSELQAEIKELRLEARQLREGIQGEDVRLLQEILATDPV
                     IYPQGLITGFFGPLTKIAVIKFQAKFGISQIGEVGPQTRAKINELLAEGAGKSGKVPP
                     GLLIAPGIAKKLGQDQDDDDDDEDDDDDETDATAPTIFSLSPLDNATLVAVDVNLVLT
                     FSENVVSGSGNVAIKKTSDDSVVEVVEASATAGDGTKTITVNPAANLAANTGYYVTVG
                     ADAFKDAAGNMFAGISDKNTWNFTTAP"
     gene            1276..1467
                     /locus_tag="VFE94_02745"
     CDS             1276..1467
                     /locus_tag="VFE94_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HZX50048.1"
                     /translation="MGDTSKRGFASMDPVRQREISRKGGKASHSGGFASMDPEKQRQI
                     ARKGGKASHGGGRRVATNW"
     gene            complement(1503..2051)
                     /gene="thpR"
                     /locus_tag="VFE94_02750"
     CDS             complement(1503..2051)
                     /gene="thpR"
                     /locus_tag="VFE94_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZX50049.1"
                     /translation="MKPHRVFIAINLPDEAKEKLLAYKTKWPEVPARWTTPENLHLSL
                     AFLGNTSDQELKEVCESMQEVGERHKPFSININRIVYGPDAKRLRMIWAVGESSHELL
                     ALQKDVEKTLEQESDQTFSPHLTLARLKAFELQRMEAEELPEVNEEISLSFDVNSLGV
                     MESAVKRSGAEYTVVQSCKLGA"
     gene            2231..2917
                     /locus_tag="VFE94_02755"
     CDS             2231..2917
                     /locus_tag="VFE94_02755"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZX50050.1"
                     /translation="MAKKRESSNKKLAEIFLHTADLLEINSVPFKPAAYRKAAKALEE
                     LEQDVLDVYKEHGVKGLEKIRGVGKSIAGKIEEFLTKKKIPFYEELKEKTALREIVTH
                     FFETKNISIKQLKMNAKKQKIIYARYTAPAKQLLELAGSVEKAKKAIDIVAAWANTRG
                     LDYTIETVFKKWLELDRLKPKEVVKKAFYRDMPMVWSEAKKKWFVVADSGEWLEYADD
                     EDKIEWKIVK"
     gene            2899..3657
                     /locus_tag="VFE94_02760"
     CDS             2899..3657
                     /locus_tag="VFE94_02760"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZX50051.1"
                     /translation="MENSEIKIAFLGREFGLVVRNRLQEAGYTLVEAANDEVDLLVVG
                     FWGNIVRKGDLEKPKYGALNVHPSLLPKYRGPTPVPFTILNGEIETGVSIIKMDEEVD
                     HGPLLAQREFSIFNYQFSNGVGKPTTPELLQVLWEMGADLLVEILPKWIAGEITPEEQ
                     DHSKATYTKKLTREDGLIDWKQNPEYIDRQIRAFYPWPGAFFFWKGKRVKILKAHVEQ
                     EKLIVDELQMEGKKPTTLNEFLLGHPDFTMPPER"
     gene            3733..>4229
                     /locus_tag="VFE94_02765"
     CDS             3733..>4229
                     /locus_tag="VFE94_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50052.1"
                     /translation="MLSYYELRKGVQFIYEGQPYEVLEFRQMGKSQDVVVAQTKIRNL
                     ITGRVLPKNFHQGDVFQEADLEKFQAKFIFANRGKFTFARADDPGKRFELTEEQVGGA
                     AKFLKSNQEVEAIIFQEKVISITPPIKVQLKVKDAPPGVQGDRSAGGTKTVTLETGAV
                     INVPL"
BASE COUNT         1148 a          961 c         1051 g         1069 t
ORIGIN      
        1 gggcgtacgg cctgctgata ggcaagagtc tttacctgct ctcgaattgc catggcttgc
       61 ttgagtactt cgtcgatttg cgttttgaga gttgcaaaat cagttcccag aacaaaagtg
      121 gggtacaaag accccactag aatgcctgta atgccaataa gagtgaaaaa ctgcttcacg
      181 gggcggtagt aaagttccag gtgtttttat cgcttatacc ggcaaacatg ttacccgctg
      241 cgtctttgaa agcgtcggca cccaccgtta catagtatcc ggtgtttgcc gctaggtttg
      301 cggcgggatt taccgtaatg gtttttgttc catctcctgc tgtcgcacta gcttcgacaa
      361 cttctaccac cgagtcgtcg ctcgttttct ttatggcaac gttcccgctg cccgaaacaa
      421 cattttcaga aaacgtgagt acgaggttta cgtcgacagc tacgagggtt gcgttgtcta
      481 gcggagaaag gctgaaaatg gttggggctg tcgcatcggt ttcgtcgtcg tcatcgtctt
      541 cgtcatcatc atcgtcatcc tgatcctggc caagtttctt ggcaatgccg ggggcaatta
      601 agaggcctgg aggaactttg ccggatttac ccgcgccttc tgctaaaagc tcgttaatct
      661 ttgcccttgt ttgcgggcct acctctccaa tctgactaat accaaacttt gcctggaatt
      721 ttattactgc gatctttgtg agagggccaa aaaacccggt tataaggcct tgggggtaga
      781 ttacagggtc tgtggcaaga atttcctgca aaaggcgtac atcttctccc tgtatgcctt
      841 cgcgtaattg ccttgcctcg agcctaagct cttttatttc ggcctgcaat tccgactgct
      901 ggccttgcag ctggcttatt tgcgacgtta actgctgaac ttgcgccaac aggctttgaa
      961 tttgcgcctg cagctgctgg ataagagctg tgtcggttgt gccctgcgca aaacttgctg
     1021 aaggaataat gcttcccgcg agaagactga aagcaacaat ccctcctaga atctgtcttt
     1081 taaacatata aaaggattaa gaaacttata atggtgatac gactcttgct catattatac
     1141 tcttttgtac acaaacgctg caatagcctc gtctgttaca cagagtttaa gacacctaat
     1201 ggtatacggt gtgttagtac catactacga acggtgaaag gtcatgggaa ttacaaatta
     1261 gttagcatat gtgctatggg agacacatca aaacgcgggt ttgcctctat ggatcctgta
     1321 aggcagcggg aaatctcgag aaagggtggg aaagcctcgc actcgggggg ctttgcttcc
     1381 atggatcctg aaaagcaaag gcaaattgca aggaaaggtg gaaaagcttc tcacggagga
     1441 ggcaggaggg tagccaccaa ctggtaaaca aaggccccga cggacagtcg gggcctttgt
     1501 ttttatgctc caagcttgca gctttggaca accgtgtatt ctgcgccgga gcgttttacg
     1561 gcactctcca taacccctag ggagtttacg tcaaaagaaa gcgagatctc ttcgttgacc
     1621 tctggtaact cctccgcttc catgcgctgc agctcaaatg ctttgagccg tgccaaagta
     1681 agatgcggtg aaaatgtttg atcactctcc tgttccagag ttttctctac gtctttttgc
     1741 aaagccagaa gttcatgcga actttcgcct actgcccaaa tcatccgcag cctttttgcg
     1801 tcgggcccgt atacaattcg gttgatgttg atggaaaatg gtttgtgccg ctcccctacc
     1861 tcctgcattg actcgcaaac ttccttcaat tcctggtcag acgtattccc caaaaacgcc
     1921 agcgacaaat gcaggttttc tggcgtagtc cagcgtgccg gtacctccgg ccattttgtt
     1981 ttgtatgcca gcaatttctc tttagcctcg tctggcaagt ttatggcaat aaatacccta
     2041 tgcggtttca tagttaagct tactttatcg taattttttg gccatttcaa cataaggtac
     2101 ctaagtcggg cgatcgcctg atttaggtac ctatgtggat gtttaacgcc gggctttaaa
     2161 cattttgccc aaaaagaaaa ggccccgacc gattgaagtt tgaggaaaag aaggctagaa
     2221 ttgaagtgca atggcgaaga agagagaatc aagtaacaaa aaactggcgg agatattttt
     2281 gcatacggca gacttgctgg agattaacag cgtgcctttt aagcctgccg cctatcgcaa
     2341 agcggcaaag gctttggaag agttggagca agatgttttg gatgtgtaca aagagcacgg
     2401 cgtgaaaggg cttgaaaaaa tccggggcgt tgggaaaagt attgcgggta aaatcgaaga
     2461 gttccttacc aaaaagaaaa tcccctttta cgaggaactc aaagaaaaaa ccgcgttgcg
     2521 cgaaattgtt acgcactttt ttgaaacaaa aaacatttcc ataaagcagc ttaaaatgaa
     2581 cgccaaaaag caaaaaatta tttacgcccg ctatacggct cccgcaaaac aattgcttga
     2641 gcttgcgggt tccgtggaaa aagcaaaaaa agcaattgat attgtggccg cctgggcaaa
     2701 cacgcgcggg ttggactaca ccatagaaac cgtgtttaaa aagtggctgg aactcgaccg
     2761 cctaaaaccc aaagaggtgg taaaaaaggc gttttaccgc gacatgccca tggtgtggtc
     2821 ggaggcaaag aaaaaatggt ttgtggtggc agacagcggg gaatggcttg aatatgccga
     2881 cgacgaagac aaaatagaat ggaaaatagt gaaataaaaa ttgcgttttt gggtagagaa
     2941 tttgggttgg tcgtacgaaa caggcttcag gaagctggct acacgttagt ggaggccgca
     3001 aacgacgaag ttgatttatt ggtggttggg ttttggggaa atattgtgcg aaagggagat
     3061 ttggaaaaac caaaatatgg cgcgttaaac gtgcaccctt ccctattgcc taaatatcgg
     3121 ggtcccacgc ccgtgccctt taccatttta aacggcgaaa tcgaaaccgg agtaagcatt
     3181 attaaaatgg acgaagaggt tgatcacggg ccgttgctgg cgcaacggga attttcaatt
     3241 ttcaattatc aattttcaaa cggcgtcggc aagcccacta ccccggaact tttacaggtt
     3301 ctttgggaaa tgggagctga tttgctggta gaaatattgc ccaagtggat tgcgggcgaa
     3361 attacgcccg aagagcagga tcacagcaaa gcaacgtaca cgaaaaagct taccagagaa
     3421 gatggcctta ttgactggaa gcaaaatcca gagtatatag ataggcagat tcgggcgttt
     3481 tacccttggc ctggcgcatt cttcttttgg aagggcaagc gcgtaaaaat tctcaaagcg
     3541 catgtggaac aagaaaagct gattgtcgac gagctccaaa tggaaggcaa aaagccaact
     3601 acgctaaacg aatttttgct cggccatccg gattttacaa tgcctcccga gcgataaaat
     3661 tttgctcgcg ctttacccca gagggaccct acgcgtctca caaaatttca ccgctcgtcc
     3721 ggctgcaaat ctatgttgtc atattatgag ttgagaaaag gcgtgcaatt catttacgag
     3781 ggccagccct acgaggtttt ggaattccgc caaatgggaa agtcgcaaga cgtggtagtg
     3841 gcgcaaacca aaatccgcaa ccttattacg ggccgcgtgc ttcccaaaaa ctttcaccag
     3901 ggcgacgtgt tccaagaggc agaccttgaa aaattccagg caaagtttat ttttgccaat
     3961 cgtggaaagt ttacatttgc acgcgcggac gaccccggca agcgttttga gcttacagaa
     4021 gagcaggtcg gaggcgccgc caaattttta aaatccaacc aggaggtaga agcaattatt
     4081 tttcaagaga aagttattag tattactccg cccataaagg tacagctcaa agtaaaagac
     4141 gctcctccgg gcgtgcaggg cgaccgctcc gcgggcggca ccaaaaccgt aaccctggaa
     4201 accggcgccg ttataaacgt gcccctgtt
//
LOCUS       DASRUP010000072         5450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_813106, whole genome shotgun sequence.
ACCESSION   DASRUP010000072 DASRUP010000000
VERSION     DASRUP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813106"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFE94_02770"
     CDS             <1..385
                     /locus_tag="VFE94_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZX50053.1"
                     /translation="QILRVEGKGDAGRRKGKAGDLYIRIVVKKHPLFERRGDDVVLKK
                     DIAFSQAVLGDEIETPTLEGTNFLLKIPEGAESGEVLRIKGKGIPHFSGLGRGDMYVQ
                     LNIATPKKLSKEQKEALERLKDLGI"
     gene            404..475
                     /locus_tag="VFE94_02775"
     tRNA            404..475
                     /locus_tag="VFE94_02775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:436..438,aa:Lys,seq:ttt)
     gene            914..1207
                     /locus_tag="VFE94_02780"
     CDS             914..1207
                     /locus_tag="VFE94_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50054.1"
                     /translation="MPISAQRLFEELYGFLGEMVLRESIIDDTGEEVRIEDAIEQVVR
                     GLLQEQAAIVRLYFDERNPKALSKAAEENLKEQALENLRNPNLGLGRFKTIIG"
     gene            1234..1584
                     /locus_tag="VFE94_02785"
     CDS             1234..1584
                     /locus_tag="VFE94_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX50055.1"
                     /translation="MSYVIIVDDDPASLRMAQTMVAQLDWEYVGTSNGAEALAEAQRR
                     KPDLFLSDTNMPGMGGIELLKIVESDENFRGIPVILMSGENREIEARENGAAAFIQKP
                     FRLEDLRAAIEKAM"
     gene            1684..2736
                     /gene="dinB"
                     /locus_tag="VFE94_02790"
     CDS             1684..2736
                     /gene="dinB"
                     /locus_tag="VFE94_02790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZX50056.1"
                     /translation="MANRIIILLDMDYFFAQVEERESPQFKGKPVVVGADPKEGKGRG
                     VVSTANYVARKYGIHSAMPISKAWQLCPTAIFLPPNFELYSRTSENIMNIIRGFSDKV
                     EQTSLDEAYIDVSFCKTYKAAEKIAKDMRKEILEKERLTCSCGVGPSKMIAKIACEMA
                     KPDGIGVVTEKEAEKFVEPLDIEKMPGIGQKTAAILRSSNFQTVADIKKLSEHDLEDL
                     FGVRGKEMYFNVRGIDNSQVETEREAKSIGKEVTFETDTRDPELLVLTFEQLCRQVAQ
                     ELKEEGQAFKTVTVVCRFSGFETHTKSKTFKEASYDQGLLRAEATKLFLRFLVEKQKP
                     LRLIGVRVQLAPLTKA"
     gene            2750..3949
                     /locus_tag="VFE94_02795"
     CDS             2750..3949
                     /locus_tag="VFE94_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZX50057.1"
                     /translation="MIRNILIFVLFLGVLGSFAGGFYIGKIYGYVAPIEGVTNLEDGK
                     PGDVDFSLFWDAWRVIQEQYVGPEPLNYEAMVHGAVKGMVESLGDPYTTFLDPKDTKL
                     FLEDLSGSFGGVGMEIGMRESRLQIIAPLEGTPAEKAGLRAGDNILRIGEQFTNDMTI
                     DEAVSLIRGPEGSKVMLLVLRDGWDEPREFDIERATITIPSLEWEFVEGDIAYVRLFQ
                     FSEKANQDFQRAANEILASSSQKVIIDVRNNPGGFLQVAIDIAAWFVDRGDIVVIEDS
                     GDKGEREEHKARGNAAFRDHDVVVLINEGSASASEILAGALRDNNQVQLIGKKSFGKG
                     SVQELETLKDQSSLKVTIANWLTPLGNLITGKGLEPDVEVELSDEDFEANRDPQLDKA
                     IEVLKNL"
     gene            3964..4416
                     /gene="rplI"
                     /locus_tag="VFE94_02800"
     CDS             3964..4416
                     /gene="rplI"
                     /locus_tag="VFE94_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZX50058.1"
                     /translation="MKVILLQDVEKVGKKFEVKEVADGYGRNFLLARNLAKPATPDAL
                     EWLEMQKEIVSQKAEEDLKTMQDLASSLDDLEVPITVKVGEEGQLFESINAQKITERL
                     KEMGHEVKKNQVRLEEPIKELGEFPVKIHLDHNLEAEIMVILTEHPAP"
     gene            4437..>5450
                     /locus_tag="VFE94_02805"
     CDS             4437..>5450
                     /locus_tag="VFE94_02805"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZX50059.1"
                     /translation="MTKYIFVTGGVMSSVGKGVATASIGKILQARGYSVSLIKCDMYV
                     NIDAGTMRPTEHGEVFVGEDGIEADQDLGTYERFVNQVSTREHYITTGQVYAEVIKRE
                     RNLEYKGEDVEVVPDIPNEILRRIEIVRKKDKSDFIVVEIGGTVGEYQNILFLEAARM
                     LKLKRPKDVLFVLVSYLPVPGKIGEMKTKPTQYASRTLNSVGIQADFILCRSEQRIDE
                     KRKEKLAIFCNIDKDAAIAAPDVDNVYEVPLNFVKDELDLKILKRFGLKPREKDMREW
                     ASFMAKAEKLTKTVKIGIVGKYFDTGDFTLADSYISVIESLRFACWANGVKPELTWLS
                     AERL"
BASE COUNT         1584 a         1065 c         1519 g         1282 t
ORIGIN      
        1 ccagattttg cgcgtagaag gaaaagggga tgccggcaga agaaaaggca aagccggcga
       61 cctgtacatt cggattgtgg taaaaaagca cccgttgttt gagcggaggg gagatgatgt
      121 tgtgctcaaa aaagatattg ctttttccca ggctgtactg ggcgatgaaa tcgaaacccc
      181 cacgctcgag ggaacaaact ttcttttgaa aatccccgag ggagctgaat ccggcgaggt
      241 gctgcgcata aaaggaaaag gtattccgca tttttccggc ttgggcaggg gagacatgta
      301 cgtgcagcta aacattgcaa cacccaaaaa actctccaaa gaacaaaaag aagcgcttga
      361 gaggttgaaa gatctcggaa tttagggtat actatagagc gacgcgccca tagctcaatg
      421 gtagagcagt ggccttttaa gccattggtt gaaggttcga gtccttctgg gcgcacactt
      481 cctttattta gcgtaaacaa caaaagcggc tttacagccg tttttgtatt gtcgaacaaa
      541 tcttacgatc gtgaatttag ttcgacaatg cacccttgct ttttgttccg attttgcgta
      601 cgattttctt aaggaggaag tgaacatgga tgaaaacgaa aaagctaagc gcattctaga
      661 agcacttaaa aatccgccgc cggaggtcac agaaacatat caaaggaaac tcgctgaaat
      721 ggaaaagggt ttgggcggcc ccccgtcgcc agaaacactt gctcggcgat gcacctggct
      781 aggtttaacc tagtacaaga ataaggcgct ggggaggaga tagaaaaact tcttggcgcc
      841 ttttgttttg acaagagaaa gagaacgtgc tagtatattg tagacccaaa aatcaaaaag
      901 agaggtgcag aaaatgccga ttagcgctca aagactgttt gaggaacttt atggatttct
      961 cggggaaatg gttctacggg agtccataat agatgacacg ggagaggagg tgcgaattga
     1021 ggatgccatt gagcaggtgg ttcggggtct tctccaagaa caagccgcta ttgtgcgact
     1081 atactttgac gaaaggaatc caaaagctct ttccaaggcc gccgaagaga acctaaaaga
     1141 acaggctctg gaaaacctgc gaaacccgaa cctaggactt gggcggttta agaccatcat
     1201 tggatagaaa ctttataacg agaggtggtt tttatgtctt atgtaataat cgtggatgat
     1261 gacccagctt cattgcgtat ggcacagaca atggtcgctc aactagattg ggagtatgtc
     1321 ggtaccagta acggcgctga agctcttgcc gaagcccaga gaagaaagcc tgatctgttc
     1381 ttatctgata cgaacatgcc tgggatgggc ggaattgaac tactcaaaat tgtggaaagc
     1441 gacgaaaatt tccgcggcat tccagttatc ctcatgagtg gggagaatag agaaatagag
     1501 gcacgagaaa atggagctgc cgcttttatc caaaagccct tcaggttaga ggatttgcgg
     1561 gcggctatcg aaaaggcgat gtagtaggta gtccttcaat aattcttttt agtccctgcg
     1621 agtaatttgg ggaaatacgc tcgcggggac ttttattttg ggaaatggta gaattatatg
     1681 gttatggcga atcgaattat tatattgctg gacatggact atttttttgc gcaggtggag
     1741 gagcgcgaga gcccgcagtt taaaggaaag ccggtggtgg ttggggcaga cccaaaagaa
     1801 ggcaaaggca ggggagtggt gtccacagcg aattatgttg cgaggaagta tggcattcac
     1861 tctgccatgc ccatatcgaa agcttggcag ctttgcccta ccgctatatt tttgccgcct
     1921 aattttgagc tgtatagcag aacctcggaa aacattatga atattattcg tggcttttca
     1981 gataaagtag aacaaacgtc gctcgacgaa gcatatattg acgtgagctt ttgcaaaacg
     2041 tacaaggcag cagaaaaaat agccaaagac atgcgcaagg aaattctgga aaaagaaagg
     2101 cttacctgta gctgcggcgt ggggccaagc aaaatgatag cgaaaatcgc gtgcgagatg
     2161 gcgaaaccag atggaatagg ggtggtaacc gaaaaagaag cagaaaaatt tgtagagcct
     2221 ttggacatcg aaaaaatgcc gggcattgga cagaaaacag cggcaatttt gcgctcgtca
     2281 aattttcaaa ccgtggcaga tataaagaag ctttcagagc atgatttaga agacctgttc
     2341 ggggtgcgcg gcaaagaaat gtactttaac gtgcgtggta tagataatag ccaagtggag
     2401 acagaaagag aggcaaagtc cattggcaaa gaagttacct ttgaaacaga tacgcgagat
     2461 ccggaattgc ttgtgcttac gtttgagcaa ttgtgccgcc aggtggcgca agagcttaaa
     2521 gaagaggggc aggcgttcaa aacagttacg gtggtgtgca ggttctccgg gtttgaaacg
     2581 cacaccaaat ccaaaacatt caaagaagca agctacgacc aggggctgtt gcgtgcagag
     2641 gcgacaaaac tgtttttgcg ttttttggta gaaaagcaaa agcccctacg gcttattggc
     2701 gtaagggttc agcttgctcc tttaacaaaa gcatgataga ataaggcctg tgatacggaa
     2761 tattctcatt tttgttttgt ttttgggcgt gctcggcagt ttcgcaggcg gattttatat
     2821 agggaagatt tatggatacg tggctcctat cgagggagtc acgaatttgg aagacggcaa
     2881 gcccggcgac gttgattttt ctttgttttg ggatgcctgg cgcgttatcc aggaacaata
     2941 tgtggggcca gaacccctga attacgaagc aatggtgcac ggcgcagtaa agggaatggt
     3001 agaaagcctg ggagatccgt ataccacgtt tttggatccc aaagacacca agttgttttt
     3061 ggaagacctc tcgggttctt ttgggggagt aggtatggag attggcatga gagaaagcag
     3121 gctgcagatt atagctcctt tggaagggac gcccgcggaa aaagcaggct tgcgtgcagg
     3181 ggataatatt ttgcgcatcg gcgagcagtt taccaacgac atgaccattg acgaggcggt
     3241 ttctctcata cgagggccag agggaagcaa ggtaatgctt ttggttttgc gcgacggctg
     3301 ggatgagccg cgcgagttcg atattgaacg cgccaccatt acgattcctt cgcttgaatg
     3361 ggagtttgtg gaaggggata tcgcgtatgt aaggctcttt cagttttcag aaaaggcaaa
     3421 ccaggatttt cagagagcgg caaatgagat tcttgcctct tcttcacaaa aagtgataat
     3481 tgatgtaaga aataatccag gagggttttt gcaggtagca atcgatatcg cagcctggtt
     3541 tgtagaccgg ggagacattg tggttattga ggattcgggc gataagggag agcgcgaaga
     3601 gcacaaggcc cgcggaaatg cagctttcag ggaccacgac gtggttgtgc tcataaacga
     3661 agggtcggct tcggcttccg aaattcttgc tggcgccttg cgcgacaaca accaggtgca
     3721 gcttataggc aaaaagtctt ttgggaaagg atcggtacag gagttagaaa ccctgaaaga
     3781 tcagtcttcg ttgaaagtaa caatagccaa ctggctgact cctttgggaa accttattac
     3841 cggcaagggg ttggagcccg atgtggaggt agagctttca gacgaagact tcgaggctaa
     3901 ccgcgacccc cagcttgaca aggctattga agtgcttaaa aatctatgag aggatagggt
     3961 attatgaagg tcatcctgtt acaagacgtc gaaaaagtag gtaagaagtt tgaggtaaaa
     4021 gaggtagccg atgggtatgg gcggaatttc ctactggctc gaaatttagc caaacccgca
     4081 acccccgacg ccttagaatg gctcgagatg caaaaagaga ttgtttcaca aaaagcagaa
     4141 gaagatctaa aaactatgca ggatttggcc tccagcctcg atgacttgga ggtgccaata
     4201 acggtgaaag tgggagaaga agggcagttg tttgagtcga ttaatgcgca aaaaattaca
     4261 gagcgcttaa aagaaatggg ccacgaagta aaaaagaatc aggtaaggct tgaagagccc
     4321 ataaaggagc ttggcgagtt tccggtaaag atacatttag accacaatct tgaagccgaa
     4381 ataatggtta tcctaaccga acaccccgcc ccataggcgg ggtgtctctt ttttgcatga
     4441 ccaagtatat ctttgttacg ggaggcgtga tgtcgagcgt aggaaaagga gtggcaacgg
     4501 catccatagg aaaaattctg caggccagag gctattcggt gtcgctcatt aagtgcgata
     4561 tgtacgtgaa tattgacgct ggcactatgc gccccaccga gcacggcgaa gtgtttgtag
     4621 gagaagacgg cattgaggcc gaccaggatt tgggtacgta tgagcgtttt gtaaaccagg
     4681 tttccaccag ggagcactat attaccacag gccaggtgta tgccgaagtt atcaagcgag
     4741 agcggaacct ggaatacaag ggagaagacg tggaagtggt gcccgatatt cccaacgaga
     4801 ttttgcgccg cattgagatc gtccgcaaaa aagacaaaag cgattttatc gtggtggaaa
     4861 tcggcggcac ggtaggggag taccagaaca ttctgttttt ggaggctgcc cgcatgttga
     4921 aattaaagcg ccccaaagac gtgctgtttg tgctggtaag ctacttgccc gtgccaggca
     4981 aaattggcga aatgaaaaca aagcccaccc agtatgcttc ccgcacgtta aactcggtgg
     5041 gaattcaggc cgactttatc ttgtgccgtt cagagcagcg catagacgaa aagcgcaaag
     5101 aaaagcttgc cattttttgt aatatcgaca aagacgcggc cattgccgcc cccgacgtgg
     5161 acaacgtata cgaagtgccc ttaaactttg taaaagacga gctggatttg aaaattttaa
     5221 agcgctttgg gctaaagccc cgggaaaaag atatgagaga gtgggcaagc ttcatggcaa
     5281 aggcagaaaa gcttacgaaa acggtgaaga tcggaatcgt aggaaagtat tttgatacgg
     5341 gcgactttac tttggcggat tcctatattt ctgttattga gtctttgcgg tttgcctgtt
     5401 gggcaaacgg cgtgaagcca gagcttacct ggttatcggc cgagcgtttg
//
LOCUS       DASRUP010000073         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_587377, whole genome shotgun sequence.
ACCESSION   DASRUP010000073 DASRUP010000000
VERSION     DASRUP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587377"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /gene="carB"
                     /locus_tag="VFE94_02810"
     CDS             complement(<1..1914)
                     /gene="carB"
                     /locus_tag="VFE94_02810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZX50060.1"
                     /translation="MQKGKIKKIIVLGSGALKIGQAGEFDYSGSQAIKALKQEGVETV
                     LINPNIATVQTDEGLADKVYFLPVTPYFVQRVIEKEKPDSIALSFGGQTALNCGLALA
                     EQKVLAKYNVQVLGTPLKAVRETEDRDLFRQKLQEIQVEIPKGKAITTPKQGLGFAKE
                     VGYPVFVRLGFALGGQGSGIARTEKQLQEILHNAFGQSPSVLIEEYLEGWKEVEYEMM
                     RDSAENCIAICNMENIDSMGIHTGESIVVAPSQTLGNTEYHFLRQICLRVVEKFGIIG
                     ECNVQLALSPTSMEYRVIEVNARLSRSSALASKATGYPIAFAAAKVGLGYTLDQLDNK
                     ITQTTKAFFEPALDYVVVKIPRWDLQKFRYVKKEIGPEMKSVGEVMAIGRTFEEALQK
                     AVRMLAKGLHGVVGDNNFNGDLEPYLRIPHEKRFIAAAKAIEQGWSIEKLSSLMQIDA
                     WFLQKLDRIVEIKKKLETQKLTKELLLQAKQAGFSDWQVGKFTGNKEGEEIREIRKKW
                     DLHPWVKQIDTMAAEWPAKTNYLYTTYNATSHDVEFPRQKSVLILGSGAYRIGSSVEF
                     DWCCVQAGKAAQELGYKTIMLNYNPETVSTDFDQCDRLYFDEISLERVQDIYELENPK
                     GMIVSMGGQIPNNI"
     gene            complement(1896..2981)
                     /gene="carA"
                     /locus_tag="VFE94_02815"
     CDS             complement(1896..2981)
                     /gene="carA"
                     /locus_tag="VFE94_02815"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZX50061.1"
                     /translation="MERKRQKAKLVLEDKSFFEGFLFGHPRSAAGEVVFQTGMVGYPE
                     AMSDPSYFGQILTFTFPLIGNYGVPSQEKSAEGFFLHFESGRIQPKGIIVQDYSFAYN
                     HWQAVQSLEQWMLQNKVPGMYGIDTRALTKKLREKGTMLGKIIVGAQKINAYDPNATD
                     VLGEVTCKDPTEYGTGGPRVLLIDCGAKEGIVRSLRSRGATVVRVPYDADISKYQYDG
                     ILISNGPGDPKKAAKTIAQIKKALLQTKPVFGICLGSQLLGLAAGGDTEKLKYGHRSQ
                     NQPCKNLQTGKCYITSQNHGFAVTEKSLPKDWQVWFQNINDHTVEGVKHKTKPFFSVQ
                     FHPEANPGPLDANFLFDDFLNLCKKAK"
     gene            complement(3065..3694)
                     /locus_tag="VFE94_02820"
     CDS             complement(3065..3694)
                     /locus_tag="VFE94_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50062.1"
                     /translation="MVQNGLYLAERPNEKEERPPYTDLSLTLRAFLKHSRKPPVWYHT
                     IAWGRLIAGAHEVRKEAISYRNFKVGCGVAATKNGRQYAFFAANRKPLKEWGKICAEG
                     LAIDAALEAGCDSINGLVVIGAPQIDHGSGMLSSVLHPCEKCRPMFLKLPQISPDTFF
                     LGISARNEVKPLEDDYPLGLPLAAKELWRLGSLLAAHERAQMGRYELIH"
     gene            complement(4244..>4394)
                     /locus_tag="VFE94_02825"
     CDS             complement(4244..>4394)
                     /locus_tag="VFE94_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZX50063.1"
                     /translation="KKEAIIMHPLPRVDEISQAVDADPRAAYFRQAQNGLYVRMALLH
                     YLLSK"
BASE COUNT         1022 a         1091 c         1023 g         1258 t
ORIGIN      
        1 aatgttgttg gggatttggc cgcccatgga tacaatcatg cctttggggt tttccagctc
       61 gtatatatcc tgcacgcgct ctaaagaaat ttcgtcaaag tacagccggt cgcactggtc
      121 aaagtcggtg cttacggttt ctgggttgta gttgagcatt atggttttgt agcccagttc
      181 ttgcgctgct ttccctgcct gcacgcagca ccaatcgaac tccacagacg agccaatgcg
      241 atacgcaccc gaccccaaaa taagcacgga cttttgcctg ggaaattcca catcgtggct
      301 ggtggcgttg tacgtagtgt acaaataatt tgtttttgcc ggccattcgg cagccatggt
      361 gtcgatttgt tttacccatg ggtgcaaatc ccactttttg cggatttcac ggatttcttc
      421 gccttctttg ttgcccgtaa acttccctac ctgccagtct gaaaacccgg cttgttttgc
      481 ctggagcagc agttctttgg taagtttttg cgtttccagt tttttcttta tttccacaat
      541 gcgatctaac ttttgcaaaa accatgcgtc tatttgcata agcgaagaaa gcttttcgat
      601 agaccaacct tgctcaatgg ctttggctgc ggcaataaaa cgtttttcgt ggggaatgcg
      661 caagtaaggc tccaagtctc cgttaaagtt gttgtcgccc accacgccat gcaagccttt
      721 tgccagcatg cgcaccgcct tttgcagagc ctcttcgaaa gttctgccaa tggccataac
      781 ttcgcccacc gacttcattt caggcccgat ttctttttta acatagcgga atttttgcaa
      841 atcccagcgg ggaattttta ccactacgta gtctaacgcc ggttcaaaaa acgctttcgt
      901 ggtctgggta attttgttgt cgagctgatc gagcgtatag cctaagccta cttttgccgc
      961 cgcaaatgca atagggtatc cggtggcctt gcttgccagc gccgagcttc gggaaagcct
     1021 ggcgtttact tcgataacgc gatactccat agaggttggc gaaagagcca attgcacatt
     1081 gcactcccct attatgccaa acttttccac tacgcgaagg catatctggc gcaaaaaatg
     1141 atactcggtg ttgccaagcg tttgcgaagg agccaccaca atgctttctc ccgtgtgaat
     1201 gcccatggag tcgatatttt ccatgttgca aatggcaatg cagttttctg cgctgtcccg
     1261 catcatttcg tattccactt ctttccaacc ttccagatat tcttcgataa gcaccgacgg
     1321 gctttggcca aacgcgttgt gcaaaatttc ctgcagttgt ttttcggttc tggcaatgcc
     1381 cgacccctgg ccgcccaagg caaagcccag tcgaacaaac acggggtagc caacctcttt
     1441 tgcaaagcca aggccctgtt ttggagttgt gatggctttc cctttgggaa tttcaacctg
     1501 tatttcctga agcttttggc ggaacaagtc gcgatcctct gtttcccgaa cagcctttag
     1561 aggggttccc aatacttgca cgttgtactt tgccaacact ttttgttcag ccaatgccag
     1621 cccgcaattt aacgcggttt gtccgccaaa ggaaagcgca atggagtcgg gcttttcttt
     1681 ttcgataacg cgctgtacaa aatacggagt aaccggcaaa aagtacacct tgtcggctaa
     1741 gccttcgtcg gtttgcaccg tggcaatgtt ggggtttatt aaaacagttt ctacgccttc
     1801 ttgttttaat gctttaatcg cctgggagcc agaataatca aattcgccgg cctggccgat
     1861 tttcaaagcg cccgatccta acactatgat tttttttatt ttgccttttt gcataagttc
     1921 aaaaagtcgt cgaataaaaa gttggcgtcc aaagggccgg ggttagcttc gggatgaaac
     1981 tgcaccgaaa aaaacggctt ggttttgtgc tttactccct ctaccgtatg gtcgtttatg
     2041 ttttgaaacc aaacctgcca gtctttgggc aagctttttt cggttacggc aaacccatgg
     2101 ttttgtgaag taatatagca cttgccggtt tgcagatttt tgcacggctg gttttgagac
     2161 cggtgcccgt actttaattt ttcggtatcc cctcctgcag ccaaaccaag caactggctg
     2221 cccaaacaaa tgccaaatac cggttttgtc tgcaaaagag cttttttgat ttgcgcaata
     2281 gttttggcag cttttttggg gtcgccaggc ccgttggaaa ttaaaatgcc gtcgtactgg
     2341 tattttgaaa tgtctgcgtc atagggtacg cgtactacgg tggccccacg gctacgcaac
     2401 gagcgcacaa ttccctcttt tgctccgcag tcgataagca atactctcgg gccacctgtt
     2461 ccgtattctg ttggatcttt gcacgttact tcgccaagca catcggtagc gtttggatca
     2521 taggcgttta ttttttgcgc gcccactata atcttgccaa gcatagtgcc tttctcgcgc
     2581 agctttttgg taagtgcccg agtgtctatg ccatacatgc ccggcacttt gttttgtaac
     2641 atccattgtt ccaaactctg cactgcctgc cagtggttgt acgcaaacga gtaatcctgc
     2701 acaataatgc ctttgggctg aatgcggcca gattcaaaat gtaaaaaaaa tccctccgca
     2761 gatttctctt gcgaagggac gccatagttg ccaataaggg gaaaagtaaa tgtgaggatc
     2821 tggccaaagt acgaagggtc gctcatggct tcgggatacc ccaccatgcc tgtttgaaag
     2881 accacttccc cggccgcgga tctcgggtgg ccaaacaaaa atccctcaaa gaaggacttg
     2941 tcttctaata ctaatttcgc tttttgtctt tttctttcca tgggtgacac aaattttacc
     3001 attgtaccca ccccaacctt tcctgtcaac catgattcat ccaaaaagaa aagcgctaga
     3061 actattagtg tatgagttcg tagcgcccca tttgtgccct ttcgtgggcg gcaagtaagc
     3121 ttcccaaccg ccaaagttcc ttagctgcta gcggtaaacc tagaggataa tcgtcttcga
     3181 gcggtttcac ttcgtttctc gccgatatcc ccaagaagaa ggtatcgggg gaaatctgtg
     3241 gaagttttaa gaacatgggg cggcactttt cgcatgggtg caaaacagag gaaagcatcc
     3301 cgctcccatg atctatctgg ggcgcgccga ttaccactaa accgttaatc gagtcgcacc
     3361 cggcctcgag tgctgcatct attgccagac cttccgcaca aatttttccc cattctttga
     3421 gcggcttacg gtttgcggca aaaaaagcgt attgcctacc atttttagta gccgcaacac
     3481 cacaacccac tttgaagttt cgatacgaaa tcgcttcctt ccggacttca tgcgctcccg
     3541 caattagtct cccccaggca atagtatggt accacacagg aggtttgcgg ctgtgcttca
     3601 ggaaggcccg cagcgttagc gagaggtcgg tgtatggagg cctttcttct ttttcattcg
     3661 gcctttcggc aaggtataac ccgttttgta ccacgatcgc gcgctccctt cctaatattt
     3721 tgggctattt tttagggctg gtttatgcat accataaaac agctatttgc gcaatagcca
     3781 agcaatggca tggggcttga ttttttctgt ttaatcagta taattttgac atgccctcta
     3841 tatggctata gtgcggaggt gaatatctcg tgaggcgaca gagaagaggt tatcttcaca
     3901 taatcaagat aatcacacaa agaccagatg ggaaaactgg aaaaatttat gcgggggttg
     3961 ttgcgccaac aaagttcttt cccgaagacg ttatccggga atacggcgaa attaagggtt
     4021 tcgccaccaa gcttgagtcg tttaagcctc cctttgaaac gctcgggatg tttaggaagg
     4081 cttttccata caaaggtggg ccatgggaag ttattttcga aaccgaacct ggggtaagtg
     4141 aatctgggag atcggttacg ttcctttgga acattctcgt tttgtctaaa gggtaggaca
     4201 caaaagtgct ccgagcaaga acaacaccgc tcggggcttt attttacttg gataaaagat
     4261 agtgtagtag agccatgcgc acatacaggc cgttttgggc ttggcggaaa taggcggcgc
     4321 gggggtctgc atccactgcc tgagagatct cgtctacccg gggcaaaggg tgcataatga
     4381 ttgcttcttt tttc
//
LOCUS       DASRUP010000074         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_941819, whole genome shotgun sequence.
ACCESSION   DASRUP010000074 DASRUP010000000
VERSION     DASRUP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941819"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..286
                     /locus_tag="VFE94_02830"
     CDS             86..286
                     /locus_tag="VFE94_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZX50064.1"
                     /translation="MEQGTIVRLTDRGFGFISREGQEKDLFFHSNELRGVEFNELRVG
                     DKVTFEVAESPKGPNATNVSRA"
     gene            410..607
                     /locus_tag="VFE94_02835"
     CDS             410..607
                     /locus_tag="VFE94_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HZX50065.1"
                     /translation="MKSTPEVEQYIQGVTFPASKDELLVQARENDAPVEVISALEDLP
                     VDKFEGAEEVEKAMGATAGNS"
     gene            complement(644..1048)
                     /locus_tag="VFE94_02840"
     CDS             complement(644..1048)
                     /locus_tag="VFE94_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HZX50066.1"
                     /translation="MAYARPRQSKYELRIGKSYAGLGLFAQQPIPRGAFVIEYFGPFL
                     SSKEADTKGGKYLFEVDKDLVVDGTSRKNLARYLNHACKPNCYAEIDGERIFIYTRRR
                     IGEGEELTYHYGKGYFEDHIKPTGCKCASCRN"
     gene            1110..2210
                     /locus_tag="VFE94_02845"
     CDS             1110..2210
                     /locus_tag="VFE94_02845"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HZX50067.1"
                     /translation="MYQITVARAKAFYRKYERTISPVAFAAGFVWDNITLQRIDLLYE
                     NLTFAWNLVLALAVIAVINAYEVGYLRNRFTNRYVTFLPILLQFSFGGLFSAFIVFYV
                     RSGSLWVSWPFLLFLTLLFVGNDFFRKRYLRLTFQLSIFFIALFTYSVFVLPVLVGKM
                     GTGVFLASGVLALLVTALILLAFMRFMPGRLERNKKALLLSIGGMYLAFQLMYFANII
                     PPIPLSLKESGMYHSIELTSEEGYLYEVQFEPAPWYFFKEASDTLHVEPGAKVYHYNA
                     VFAPTRINTTISHRWLYFSEQKDEWVQISLLPFPIAGGRDGGYRGYSFISNAAPGKWR
                     VDVVTGRGQVLGRRMFTVIAADQPPQLETGFR"
     gene            2316..3254
                     /locus_tag="VFE94_02850"
     CDS             2316..3254
                     /locus_tag="VFE94_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZX50068.1"
                     /translation="MQAIPGGVFGKIESQGMYKFNANVRETPGMSAILGVLPVGSEVD
                     ILEEQAGWYHIKHQETNLEGWTASWIVERKESAPAATQAEPAAPTASAEKRYGVVQRN
                     SNVRTGPMQTVLAVLPEGTRVEILAEENGWSRIVYEGEKEGWIASWLLEPSIDKAALN
                     EYWLAKVNELRQVAGLPILETKEALLNTAATWAAHLASTGQITHTRPDGSSAHSWIAK
                     QGIAFTERYAPGGWKTNYFTENIGFRYSSLAMAEAKLALDSIFASYVAEAPWNGAHYQ
                     TLYHPDWNSVGAGFSFDPLGNGKYKLYAVFHYGSLP"
     gene            3302..3841
                     /locus_tag="VFE94_02855"
     CDS             3302..3841
                     /locus_tag="VFE94_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX50069.1"
                     /translation="MEQEDIKLIEAARSDKAAFEALYQKYTQKVYNYFWYRVGHNKEI
                     AEDLMQQTFLKAFQHLQEFEVRDASYLTYLLKIAHNTLVNYYRVPKPIPLSLLHEIPG
                     ESSFRIHKRHDATLLWRAVRQMPEAEKNALLLKYQKELSIKEVAHIMEKSENAVKLIL
                     SRARKKLASNPRLSRSPSF"
BASE COUNT          997 a          855 c         1023 g         1003 t
ORIGIN      
        1 gggccagaac tccaagaaaa acgaagtcgg gttttagtct ggagccagct gcgtccgata
       61 gggtaacgag ctagtttctt ttaaaatgga acaaggaacc atagttcgac tcacagaccg
      121 cggatttgga ttcatctctc gcgagggcca ggagaaagat ttattctttc actccaatga
      181 gctccgaggc gttgaattca atgagcttcg agtaggcgac aaagtcacct tcgaggtggc
      241 agaaagcccc aaagggccca atgctaccaa cgtgagccga gcataacaca aaaacacccg
      301 gagcttgtcg aagggtgttt ttgttatggc tagaaaggat gaaacgtttt tggttttggt
      361 gtgtttagta ttgtggtcga ataataacca tttattctcc cgcaatacta tgaaaagcac
      421 gccagaagta gaacaataca tccagggggt aactttcccc gcttccaaag acgagctgct
      481 tgtgcaggcc cgagagaacg acgctcccgt agaggttatc tctgcgctgg aagatttgcc
      541 ggtggataaa tttgaagggg cggaagaggt agagaaagca atgggagcaa cggcaggaaa
      601 cagctaggat gaaaaacgag caaccgtgtg gttgcccgtt tttttagttt cgacaagaag
      661 cgcacttgca gccggtaggt ttgatatggt cttcaaaata acccttgccg tagtggtagg
      721 tgagctcttc cccctcgcca atgcggcgcc ttgtataaat aaaaatacgc tccccgtcta
      781 tttccgcata gcaatttggc ttgcacgcat ggttgaggta acgggcaagg ttttttctcg
      841 atgtgccgtc gactactaag tctttgtcaa cttcaaaaag gtatttgccg ccttttgtgt
      901 ctgcttcttt gctcgaaaga aaagggccaa aatattctat aacaaaagct ccccgcggaa
      961 tgggctgttg ggcaaaaagc ccaaggcctg cgtagctttt gcctatcctt aattcgtatt
     1021 ttgattgtct ggggcgggcg tatgccatac cgagataaga gtataccaga ttccttgagc
     1081 cttgcaaggt tatgcagtag agtaaaggaa tgtatcagat tacggttgca agggcaaagg
     1141 cgttttaccg gaaatacgaa cggactatct cgcccgtggc ttttgctgct gggtttgtgt
     1201 gggacaacat tacgcttcag agaatcgacc tgctttatga gaacctcacc tttgcgtgga
     1261 acctggtatt ggcgcttgca gtaattgcag taataaacgc ctacgaggta gggtacttgc
     1321 gaaaccgctt taccaaccgc tatgtaacgt ttctgcccat tctgctgcag ttttcgtttg
     1381 gagggctgtt ttcggccttc attgtgtttt atgtgcggag cggatctttg tgggtaagct
     1441 ggccgtttct gcttttctta acactattgt ttgtgggcaa cgactttttc cgcaaacggt
     1501 atttgcgcct cacgtttcag ttgagcattt tctttattgc tctttttacg tattcggtgt
     1561 ttgtgctgcc cgtgctcgtg ggcaaaatgg ggacaggggt ctttctggca agcggcgttt
     1621 tggctctttt ggtaacggcg cttattcttc ttgcttttat gcgcttcatg ccgggcaggt
     1681 tagagcgcaa caaaaaagcg ctccttttga gtatcggggg catgtacctg gcgttccagc
     1741 ttatgtattt tgccaacatt attccgccca ttccgctttc cttgaaagag agcggaatgt
     1801 atcattctat tgaactcaca agcgaagaag ggtatttgta cgaagtgcaa ttcgaaccgg
     1861 ccccgtggta ctttttcaaa gaggcgtctg acaccctgca tgtggaaccg ggagctaaag
     1921 tgtaccacta caacgccgtg tttgcgccca caagaattaa taccaccatt tcgcaccgct
     1981 ggctgtattt ttcagaacaa aaagatgaat gggtccagat aagtcttctt ccgtttccta
     2041 ttgcaggggg gcgcgacggg ggctatcggg ggtatagttt tatcagcaac gcagctcccg
     2101 gcaagtggcg tgtagacgtg gttacgggcc gcggccaggt gctgggccgc agaatgttta
     2161 cggtgattgc ggcagaccag ccaccgcagc tcgaaaccgg ctttcgctag gggtctgact
     2221 accccatgtt tgccttgggg ggtagcaaga gggtataata tagggaaatg agcaattcgt
     2281 taaagttttg gtccgtttta gttttacttt gcgttgtgca agcgatacct ggaggcgtgt
     2341 ttgggaagat agagagccaa ggcatgtaca agttcaacgc aaatgtaaga gaaacgccag
     2401 gaatgtctgc cattcttggc gtgcttcccg ttggatctga agtggacatt ttggaagagc
     2461 aagcaggctg gtaccacata aaacaccagg agaccaatct ggaaggatgg actgcttcat
     2521 ggattgtgga gcgtaaggaa agtgcgccag ctgcaacgca agcggagccc gctgccccga
     2581 ctgcaagcgc agaaaaacgc tatggcgtgg tgcagcggaa ctcaaacgtg agaacaggcc
     2641 ctatgcaaac ggttcttgcg gtgttgcccg agggaactcg agtggaaata ctggcagagg
     2701 aaaacggctg gtctcgcatt gtatacgaag gggagaaaga aggctggata gcttcgtggt
     2761 tgttggaacc gagtattgat aaggcggctt taaacgaata ttggctggca aaagtaaatg
     2821 aactgcgcca ggttgcaggt ttgccaattt tggaaacaaa agaagcgctc ttgaataccg
     2881 cagccacatg ggctgcgcat ttggcaagta ccggccagat tacgcatacc aggcccgatg
     2941 gttcttctgc acactcatgg attgcgaaac aaggaattgc atttaccgaa cgttacgctc
     3001 caggcggctg gaaaaccaac tatttcacgg agaatatcgg atttcgctac agctctttag
     3061 ccatggccga agcaaaactc gccttagaca gcatattcgc ctcttatgta gcagaagctc
     3121 catggaacgg cgcgcactat caaactttgt accatcccga ttggaacagt gttggagccg
     3181 gtttttcttt tgatccttta ggaaacggca agtacaagct gtatgcggta ttccattatg
     3241 gaagtttacc gtagccatgt aacaaatagt tttttgctgc gttggtatag gtacaatgac
     3301 aatggagcag gaagacatca aacttattga agcggcaagg agtgataagg cggcttttga
     3361 ggcgctctac caaaagtata cgcaaaaagt gtataactat ttttggtatc gtgtaggcca
     3421 taacaaggaa atcgccgaag atttaatgca gcaaacgttt ctgaaggcgt ttcagcattt
     3481 gcaggagttt gaggttcggg acgcttctta ccttacgtat ttattgaaaa ttgcgcataa
     3541 tactttagta aactattacc gggtgcccaa acctattcct ttaagcttgc tgcacgaaat
     3601 accgggcgaa agttctttcc gcatacacaa gcgacacgac gctaccttgc tttggcgtgc
     3661 ggtacggcaa atgccagagg cagagaaaaa cgctttgctg ttaaagtatc aaaaggagct
     3721 ttcgattaag gaagtagccc atattatgga aaaaagcgag aatgcggtaa aactcattct
     3781 ttcccgggca aggaaaaaac tcgccagcaa ccctcgtctt tccagatccc cctccttttg
     3841 aggaggtacc ggcaaaacac aaaccccgcc agtccatg
//
LOCUS       DASRUP010000075         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2244598, whole genome shotgun sequence.
ACCESSION   DASRUP010000075 DASRUP010000000
VERSION     DASRUP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244598"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFE94_02860"
     CDS             complement(<1..491)
                     /locus_tag="VFE94_02860"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HZX50070.1"
                     /translation="MKQIFIPISIIALVAVVAGGALYFYQQQQTPAPEEPGNNNVSNF
                     AECEAAGYPIMESFPRQCRTPQGQNFTEDIGNELEKADLIRVDNPRPNQEIASPLMIE
                     GEARGTWYFEASFPIRLLDEEGNELAVAIAQAQDEWMTEEFVPFKATLEFDTPETSVG
                     TLV"
     gene            complement(488..826)
                     /gene="raiA"
                     /locus_tag="VFE94_02865"
     CDS             complement(488..826)
                     /gene="raiA"
                     /locus_tag="VFE94_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZX50071.1"
                     /translation="MRIEIKYSQFQPAQDVEEYARERIGSLERFLKSFEKNHEITVFV
                     EIARSTRHHRKVDVFYAEATFETVPGGKQLRAEATAPDIRLAIDQVKEKLKKEIRRYK
                     DKLVETRRRK"
     gene            complement(843..1523)
                     /locus_tag="VFE94_02870"
     CDS             complement(843..1523)
                     /locus_tag="VFE94_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HZX50072.1"
                     /translation="MEEEQIEEFEQKPSTASRVFRAVVALAVIAGMVYWGGLYQYFFF
                     QRTSPAIEQEELEAVIDAETLVLPVTIFIIQSEGSEGSERTKQDATRLVEQADRIWEQ
                     AGINLEILAIHEAQKSDQEIELFHRNAKAFTSGLEGFDADSINVLLVHNLRGLNGLAY
                     GGIPVVAVADYTAGYDFRVLAHEIGHKLGLGHVSVKEGGLMEQGADGFELSLEEVTTA
                     RKFAKGFE"
     gene            complement(1728..2441)
                     /gene="uppS"
                     /locus_tag="VFE94_02875"
     CDS             complement(1728..2441)
                     /gene="uppS"
                     /locus_tag="VFE94_02875"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZX50073.1"
                     /translation="MEQEDKIPQHIVILPDGNRRWAKERGLDTLEGHKAGYEKLTQLC
                     HWAKDRGVKVVTAFGFSTENWNRSEREVNYLMGLLEFALRENFSSKATKNEIKELGLR
                     VRIIGQKERLPQSLQETIAEVEEHTKANTNLHLNLAISYGGRWDIVQAAKQIMQDGIK
                     PEDLTEELFGKYLSTGDLPDPDLFIRAGGEQRLSNLVLWQAAYSELYFLPKYWPDFSE
                     QDFDDALAEFARRSRRFGH"
     gene            complement(2425..2613)
                     /locus_tag="VFE94_02880"
     CDS             complement(2425..2613)
                     /locus_tag="VFE94_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50074.1"
                     /translation="MLNTPILARITEFVKDHEIQIILGVGVVLISLLSFAIGYLTAKE
                     QLKEPLRMEQTQAYGTGG"
     gene            2625..2903
                     /gene="rpmA"
                     /locus_tag="VFE94_02885"
     CDS             2625..2903
                     /gene="rpmA"
                     /locus_tag="VFE94_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZX50075.1"
                     /translation="MSTTKSSGTAKNMRDSAPKYLGVKLFAGQKAKAGQILIRQRGSL
                     FLAGKNVGTGNDYTLFALKEGIVRFVTRKKKRFDGSSRQAKEVEILPS"
     gene            complement(2900..>3646)
                     /locus_tag="VFE94_02890"
     CDS             complement(2900..>3646)
                     /locus_tag="VFE94_02890"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZX50076.1"
                     /translation="IGIVGKYFDTGDFTLADSYISVIESLRFACWANGVKPELTWLSA
                     ERLEKDTAAVKQLKGYDGILIPGGFGSRGTEGKIKAIQFCRENKLPYFGLCLGLQLAV
                     IEFARNVCGLKGATSTEFDPKASDPIIDVMPEQKVLLEEKRFGASMRLGAYPCQVEKG
                     TLAYKAYGQESISERHRHRYELNNEYRKQLEEKGMVMSGVNKERDLVEIVELKGHPFF
                     LGTQFHPEFKSRPLQAHPLFLQFVKACAKS"
BASE COUNT          867 a          992 c          797 g          990 t
ORIGIN      
        1 aacacaagcg tacccaccga agtctcgggg gtgtcaaatt cgagagttgc tttaaagggc
       61 acaaattctt cggtcatcca ctcgtcttgg gcttgcgcaa tggctactgc gagttcattc
      121 ccctcttcgt cgagcagacg aatcgggaaa gaggcctcga aataccatgt tcctcttgct
      181 tctccctcga tcatgagcgg gcttgcgatt tcctggttcg ggcgcggatt atccacccga
      241 attaaatccg ccttttcaag ctcgtttcca atatcttcgg taaagttctg cccttggggc
      301 gtgcggcact gccgggggaa actctccata atggggtatc ccgcagcttc acattctgca
      361 aaattgctta cgttgttgtt ccccggctct tctggggctg gcgtttgctg ttgctggtaa
      421 aaatacaggg ctccgcccgc gacaaccgcg accagcgcga taatgcttat gggtataaaa
      481 atctgtttca ttttcttctt ctggtttcca ctaatttatc tttgtatcga cgtatttctt
      541 tttttaattt ttcctttacc tggtctatgg ccaaacgaat atcgggtgcc gtagcttctg
      601 cgcgaagttg cttgccgcct ggaaccgttt cgaacgtagc ttccgcgtaa aacacgtcca
      661 ctttccggtg gtgccgtgtg gaacgcgcaa tttctacaaa cacggtaatt tcgtggttct
      721 tttcaaaact cttgagaaac cgctctaaag aaccaatgcg ttctctcgcg tattcttcga
      781 cgtcttgggc cggctgaaac tggctgtact ttatttcgat tcgcatatac ctttactata
      841 tattattcaa acccctttgc aaacttgcgg gcagtggtga cctcctccag cgaaagttca
      901 aaaccatcgg ccccctgctc cattaaaccg ccttctttga ctgaaacgtg gcctaaacct
      961 aacttgtgac cgatttcgtg cgccagcacg cgaaagtcgt atcctgccgt atagtcggcc
     1021 accgcaacca cgggaattcc cccgtatgcc aaaccgttca gcccacgcaa gttgtgcacc
     1081 aataatacat taatggagtc tgcgtcaaac ccctcaagcc cactggtaaa agctttggcg
     1141 tttcgatgga acagctcaat ttcctgatca ctcttttgtg cctcgtgaat cgcgagaatt
     1201 tccaggttaa tgccggcctg ttcccaaatg cggtctgcct gttccaccaa gcgcgtggca
     1261 tcttgctttg tgcgttctga cccttcagaa ccctcacttt gaataataaa tatcgtaaca
     1321 ggcagcacaa gagtttctgc atcgattacg gcttccaact cttcttgctc gatagcaggc
     1381 gatgtgcgct gaaagaaaaa gtactgataa aggccacccc aatacaccat cccggcaatc
     1441 acggcaagcg caaccactgc gcgaaaaacc cgcgaagcgg tactcggttt ttgttcaaat
     1501 tcttcgatct gctcctcttc catgtccagc cataatacca tccgaccacc caattgacaa
     1561 tttccggtgc ccagagtagt gtgaaagtgt gatgggagct aatctcgccc attacggcct
     1621 ccgctgcacc ttctacgcta gtcgatagcg tagctcaata gctcaaagaa gcccttccct
     1681 agagtcttgt acttcaagca ggaagggctt tttacgttga ggcgaaacta atggccaaaa
     1741 cggcgagagc gacgggcaaa ctcggcaaga gcgtcgtcga agtcctgctc tgaaaagtcg
     1801 ggccagtact tgggcaaaaa atagagttca gaatatgccg cctgccacag cacaaggttc
     1861 gacaagcgct gctccccgcc cgcccggata aacaagtcag ggtctggcaa gtcgcctgtg
     1921 ctcaaatatt tgccgaataa ttcttcggtg aggtcttctg gctttatgcc atcttgcata
     1981 atctgctttg ctgcctgcac aatatcccat ctccccccgt agcttatggc aagatttaaa
     2041 tggagattgg tattggcctt ggtatgttct tccacctctg caatagtttc ctgaagggat
     2101 tgcggaaggc gctctttttg gccaataatt cttacccgca agccaagttc tttgatttcg
     2161 tttttcgttg ccttactcga aaagttttcc cgcaaagcga actccaaaag acccattaag
     2221 tagtttactt ccctttctga acggttccag ttttctgtcg aaaagccaaa agcagtaact
     2281 acttttactc ccctgtcttt tgcccaatgg caaagctggg tcagcttttc gtaccctgcc
     2341 ttatgccctt ccagagtatc caggcctctt tcttttgccc atctccggtt gccgtcgggc
     2401 agaattacaa tatgttgggg tatcttatcc tcctgttcca taagcttgcg tttgttccat
     2461 gcgcaaaggc tcttttaact gttcctttgc cgtgagatac cctatagcaa aagaaaggag
     2521 cgaaatcaac actaccccca cgcccaaaat gatctggatc tcgtggtctt tgacaaattc
     2581 tgtaatcctt gctaggatag gggtattaag cataatctac ttttatgtct acaacgaaat
     2641 cttcgggaac agcgaaaaat atgcgggatt ccgcccccaa gtaccttggg gtcaaactct
     2701 ttgccgggca aaaggccaaa gcaggacaaa ttttaatccg ccagcggggc agcctctttt
     2761 tggcaggcaa aaacgtggga accggaaacg actacaccct ctttgctctc aaagaaggca
     2821 ttgtgcggtt cgttaccaga aagaaaaaga gattcgacgg ctcctcgcgc caggcaaaag
     2881 aggtagaaat acttccttct taactctttg cacaggcttt cacaaattgc aaaaagagcg
     2941 gatgtgcttg cagcggtcgt gatttaaact ccggatgaaa ctgggtaccc aaaaagaaag
     3001 ggtgcccttt tagttccacg atttccacca gatcgcgttc cttgtttaca ccagacatca
     3061 ccatgccctt ttcttccagt tgtttccggt attcgttgtt gagttcgtag cggtggcgat
     3121 ggcgctctga aatgctttcc tggccgtacg ctttataggc tagagtacct ttctccacct
     3181 ggcatgggta tgctcccagg cgcatggaag ccccaaaccg cttttcttcc aaaagcactt
     3241 tttgttctgg cataacgtcg ataatcgggt cgcttgcttt ggggtcgaac tctgtagagg
     3301 tggcaccttt caggccgcac acgtttctcg cgaactcaat aaccgccagc tgcaacccca
     3361 aacaaagccc aaagtacggt aatttgtttt cccggcaaaa ctggatagct tttattttcc
     3421 cttcggtgcc ccggctcccg aatccgcccg gaattaaaat gccgtcgtac ccttttaact
     3481 gctttactgc agcagtatct ttttccaaac gctcggccga taaccaggta agctctggct
     3541 tcacgccgtt tgcccaacag gcaaaccgca aagactcaat aacagaaata taggaatccg
     3601 ccaaagtaaa gtcgcccgta tcaaaatact ttcctacgat tccgat
//
LOCUS       DASRUP010000076        43596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_995392, whole genome shotgun sequence.
ACCESSION   DASRUP010000076 DASRUP010000000
VERSION     DASRUP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 43596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43596
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995392"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFE94_02895"
     CDS             complement(<1..594)
                     /locus_tag="VFE94_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50077.1"
                     /translation="MFRFLVAGIVVCSVSLLALQIFVSIQPAEVHAQPASGCADGTTE
                     QFLGTLPNGAEVHGCNGAVPFVNADALCAASAHVGDVVTDSGLKGVLEGTPGSAIRWI
                     HINGDPGSGLGLEANPANRACWGTLSPCASTNPSNIRPVHSINYSLYYNGTEVLAGTG
                     QAVDGVGNFGSVCIAPPPPPDVTITGPAPGSWQTGNFQ"
     gene            complement(609..3803)
                     /locus_tag="VFE94_02900"
     CDS             complement(609..3803)
                     /locus_tag="VFE94_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HZX50078.1"
                     /translation="MHRPAINTGFQFLVLATVLCGVTLLSLQVFVSLQPAEVHAQPAS
                     GCADGTTEQNFSTLPDGRTVHGCNGSVTFANADSLCAPSATVGDVVLDNGLKSALEST
                     TGSERRWIHMNGDPGPLLGGPEPAVGLCWGTQAPCTMLSSSAFGPIATFRPAHGILSS
                     LYSLYYNGTNVLGNRGNAYPSGSPLDGAICITPPPPPSVTITGPAPSSWQNGDFQVQY
                     DATFPTSPPQSQTFDYTGDSQPWTVPSGVTEITVDVRGAQGGSSTGGLGGRVQATIPV
                     PSGQTLNIYVGGAGAGPTGGFNGGGFGGASNPGQGGGGASDIRTVSGPWDDPAGLNSR
                     LVVAGGGGGRGSGPGSSVGGGGGAGGDLVGANGLPGSGGFGVGTWGGTGGEGGTQLSG
                     GGLGWPNGFAGTPGSFGQGGGGGRFNAGFRFYHGGSGGGGWYGGGGGGGGTNEDGSSD
                     DKGGSGGGGGSSYCEPPKCLNPTHTQGYQTDNGQVVITWQPTVSGVCEIETNDGGVGW
                     QNRGTVSCGANQSATVQVGASGWCIQEGQNACNVRIVGKPPSTPISQTFAYTGGVQTW
                     PVPPGVSEIEVELWGAQGERGIDKFYSGLPASGGSGGRGGYVITRLQNLTPGQVLQIF
                     VGGNGTSGFNGGGPGGGGGASDIRIGASRIAVAGGGGEGGNGFVEFDDVHLASGGSGG
                     SGGGLMGGKGGNGSVDYNVGWYQARSRPAPSPSIGGGGGDTINNQGGSAGGGSATSGS
                     FGTGGQGDSFSGGSGGGGYYGGGGGQGGGTPTNAAVGGAGGGGGSSYVDPAYEITGST
                     THQQGVRSGNGQVIIRWVESTVPSVQDERDFSIDYTKPVASFDVPPTPADGSDLAADF
                     PIKYNATDAFIDTCSLSSSNDGGGTWTDHNTGPSACGTGLTDMGLIPGWCSPGSKCFI
                     RAQATDLAGNFSEAILRYNSAFTGGLVPCGRDSDDPLTTGLDESQPCTLCHGFALVHN
                     IITFLLFPSSFNNGFAIVMLLGALMIVVGGFSIIIGNLGNPALLNRGRGIIFTTVIAL
                     LIIYGAWVAVDFALDLLGVTIWTGTSSWWQIQCG"
     gene            complement(3811..4620)
                     /gene="rsmA"
                     /locus_tag="VFE94_02905"
     CDS             complement(3811..4620)
                     /gene="rsmA"
                     /locus_tag="VFE94_02905"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZX50079.1"
                     /translation="MNFASSKTIKTVLQKHTIKPSRGLGQNFLVEDHIVQKMVVAASI
                     QPQDTVLEVGPGIGTLTQELAKHAKKVVAVEKDAKMVEILKGTLAEFKNVEVIHGDAL
                     KTQVPKQTYKVVANLPYYISAPTIRKFLEAENRPQSITLIIQKEVAQRICAKPPDMSI
                     LAVSVQFYATPKIISYIKKTSFWPQPHVDSAIIHIVPRKEKPDIDAQKFFSVVKAGFT
                     HPRKQLLGNLSAGLKRPREHMETILRKNSIDPARRAETLSVQDWINLANDL"
     gene            4697..5743
                     /locus_tag="VFE94_02910"
     CDS             4697..5743
                     /locus_tag="VFE94_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZX50080.1"
                     /translation="MRKEREEPSSSINRRARKPFFTVALGAIFLLALAGLGATVFSDS
                     SGVGARFSAPLPSTSAMHLYANQADVAETPEFVTVGETGVLATAPPVAVSPKVLGAII
                     GEANFAQQNRRELLHYAVEEGDTIKSLSERFGISQNTILWVNELSSVSPLTLGQELVI
                     LPVSGALHLVRPADTLSEIALWYQGDAEQIMEANGIASAKDIFVGDFLIIPGGIMPKS
                     LPVGRLTPLANSYFIYPVSRSHKLSQGLHAFNAVDLSNGQCNEPVYSAAGGTVQKVGY
                     HSVAGNHVRVLHPNGVVTFYGHLSGQTVEVGQKVSQGAIVGYTGHTGYTIPAGPDGCH
                     LHFEVRGASNPFVK"
     gene            complement(5782..6135)
                     /locus_tag="VFE94_02915"
     CDS             complement(5782..6135)
                     /locus_tag="VFE94_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50081.1"
                     /translation="METTDIGELKTRLEKEKERLESELASFATKDPNVKGDWDSKYPR
                     IPGSNLEEAADEVEEYSTRLHLEHSLETKLKDINDALDRIEKGTWGTCQNCGNPIATE
                     RLQISPEAATCGKCK"
     gene            complement(6145..8316)
                     /locus_tag="VFE94_02920"
     CDS             complement(6145..8316)
                     /locus_tag="VFE94_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZX50082.1"
                     /translation="MPPRQQEPFRPEQKREYLERGEVRTMAKDIARLREEEAKKEQQR
                     IAQLKAQQNAQREKDAVAKIRSAALAAKQREETRKEERMRNIQSSILPPGEENRFEAL
                     PTPPSRSKRVLVRAIIVIIFAFIVLNIGLFLYWFVFQNQSVEIPFLSQFFSQEQTTPT
                     PTPPPASGPSPQPPPQAQPAPTPSDPLGVAHTSTLLFSEKADLTSTLKQSLEADQQEG
                     FTKLVFRNRQDNTSLESANSFFGIFEVPVPEAVSNQLQQTSFFFLYSYERGNRFGIVS
                     EVADPTALWQSLRAWEPQMEQAFVPLRDFWGNPGNGYTKSFRSTSYNGVEVRFQTFST
                     QDVGIVYTIVNNRFILASSFEAIKEAIDELQNSPGALLQNQILAVENSPGLFKPPQNL
                     SLEQAIGQVLLIGFEGTYLTPELEELMKRLRPGGVLLLARNITDSQQLQKLTQELQAL
                     SLEYSSLPLLVAVDQEGGLISRIQFGKEKTAQSTIETPAQAYAVGKERARELKSLGVN
                     LNLAPVLDTTGPHDFLFERTFQTEGLEAGRLARALLAGQKSENILSTLKHFPGYGSIS
                     FNPEKKLATVQEFPDISPFAFALSSEPEFLLTSNVIYSTVDKNYPFSFSIEGMSLIRE
                     ELGFQGIILSDDLAQPALLDNYSFEEIVFLPLRAGMNILMFAETPEATQAHKLLLQQA
                     ATDETLRKQIEASANRVLDLKKKFFFPSEEPLQFNHLSQNK"
     gene            complement(8578..10683)
                     /locus_tag="VFE94_02925"
     CDS             complement(8578..10683)
                     /locus_tag="VFE94_02925"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZX50083.1"
                     /translation="MNKYTVKLGEKEITIEFPNWAEQANGNVLIRTGETLTYVTAVMS
                     RSETSNQGFFPLIVDYEERFYAAGRILGSRFIRREGRPSDEAIICARLIDRAIRPLFP
                     KNFKREVQIITTCLSWDAQNDPDVAGLLGASIALSLSDIPWKGPLAGMRVGRINGQFV
                     VNPTYEERGQSDLDFVLAAVKEGQELLFNMIEAESDEVSEEVYEQALAFAKPHLEALI
                     AFQEDLIGKLGKEKIAHEERSPIEETVRKELQNWLEPKLEHALFQGDKQARMAAVDEV
                     KKEFTAFTKEKYENAEVLSHVDSFFDEETDRIVHKGALERNQRVDDRKLDEIRPLSAL
                     AGFLPRTHGSGLFERGQTRTLSILTLGAPGDQRLLEGMEFIGKKRFMHHYNFPPYAPG
                     EVKMLRGPGRREIGHGMLAEKGLFPVIPTPEEFPYTIRIVTEVVSSNGSTSMASVCSS
                     TLALMDAGVPIKRPVAGISMGLMLGQNGEYKLLTDIQGPEDHHGDMDFKVAGSKQGVT
                     AIQLDVKVRGITEHIFKETLAKAKKAHSHILDEVIAKILAEPRKELSPWAPKIATLQI
                     NPEKIGLLIGPGGKTINKIIDEFDVQIDIEETGLVFVTGQDAAQLEQALETVRNLTRE
                     VQVGEIFQGKVKRIMEFGAFVEVGGGQEGLVHISQLAPYRVNKVQDIVKLGDIIPVKV
                     IEIDAQGRINLSLKEAKEQ"
     gene            complement(10680..11210)
                     /locus_tag="VFE94_02930"
     CDS             complement(10680..11210)
                     /locus_tag="VFE94_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZX50084.1"
                     /translation="MSLKNKDQRVTVFIDVQNLYHSAKNLHQARVNFREIVKNAVAGR
                     KLIRAFAYVVRTKTGEEKPFFDALTSVGIETRVKDLQEYYGGLKKADWDVGIAVDAIK
                     TADSADAVVLVSGDGDYVPLVEYLKNHGKRVEVMAFGKSTSSRLREVADEFVDLEEEE
                     DKYLLKKSRWPLKITR"
     gene            complement(11223..11489)
                     /gene="rpsO"
                     /locus_tag="VFE94_02935"
     CDS             complement(11223..11489)
                     /gene="rpsO"
                     /locus_tag="VFE94_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZX50085.1"
                     /translation="MLQTKDKQKIIEKYKTHAIDSGSPEVQVGILSEEIRKLLSHLKK
                     HAKDFHSKRGLLKMVARRRTLLAHLKREDEKRYKSLLKKTGLKK"
     gene            complement(11530..11600)
                     /locus_tag="VFE94_02940"
     tRNA            complement(11530..11600)
                     /locus_tag="VFE94_02940"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11566..11568),aa:Lys,seq:ctt)
     gene            complement(11650..12192)
                     /locus_tag="VFE94_02945"
     CDS             complement(11650..12192)
                     /locus_tag="VFE94_02945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZX50086.1"
                     /translation="MYIPIILGTAREGRRSEKAANFVLREFQKQEGVETELIDVRDYR
                     IAATDDTEEQEPAKRFSEKVSRADGLIIVAPEYNHGYPGELKMMLDMLYEEYNRKPLG
                     VCGVASGGMGGVRMVEKLRLVAIELQMVPIRTAVYFSNVKTLFDEQDNITDPSYPERL
                     QKFFGELLWYAKALKAAREP"
     gene            complement(12208..12759)
                     /locus_tag="VFE94_02950"
     CDS             complement(12208..12759)
                     /locus_tag="VFE94_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50087.1"
                     /translation="MNNFKDLIAFVDKAVRNRKYPKNTGHGIKRALQLFQEELNEDEL
                     NSVDKFEENIEQIYKSVCSKHGGKDFTDTSLATYKSRVQKVLKDYKTYGIDPTKMASW
                     TPRVVQRTKRSIPNAVQKGKGSIEDHEIANAQAIVRIDLPLRGNSQKFVIEVPWDITK
                     NEVATLTAVLGSLSSKDEKNLNL"
     gene            complement(13005..13075)
                     /locus_tag="VFE94_02955"
     tRNA            complement(13005..13075)
                     /locus_tag="VFE94_02955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13041..13043),aa:Ala,seq:cgc)
     gene            complement(13077..13742)
                     /locus_tag="VFE94_02960"
     CDS             complement(13077..13742)
                     /locus_tag="VFE94_02960"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZX50088.1"
                     /translation="MRINVFLAQQLGISRRAADKLIADKKVQVNGSLAKLGQLVDPAK
                     DRVSANGKTLQSKQKESITVALYKPKGYITTRSDPKKRKIVMSLLPREFQDLKPAGRL
                     DYESEGLLVLSNKGDLIYKLTHPKYEKQKEYRLVFKKAVSKELINAFLKGIRLTEGIA
                     KADKVTQIDKNELEVIIHQGWNRQLRRMAEACHYEIAHLIRTRIGDIKLENLKPGEWR
                     KIK"
     gene            complement(13748..13819)
                     /locus_tag="VFE94_02965"
     tRNA            complement(13748..13819)
                     /locus_tag="VFE94_02965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13785..13787),aa:Pro,seq:cgg)
     gene            complement(13821..15269)
                     /locus_tag="VFE94_02970"
     CDS             complement(13821..15269)
                     /locus_tag="VFE94_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZX50089.1"
                     /translation="MKAIPKEVQEIGETLEKQGFEAYVVGGCVRDLLLGREPEDWDVT
                     TQARPEELQKIFPESFYENKFFTVTVLTQSQNPKLKEIEITTYRSDHKYTDRRRPEEV
                     KYAQTLQEDLSRRDFTVNAIALRGKEIIDPFGGQEDLKAKIIRAVGEPEARFQEDALR
                     MMRAARLATQLGFFIEPKTKEAIQKNAALLQEISKERIRDEFCKIVMAKQAMAGIELL
                     RELKLLSFIAPELEEGYEVSQNKHHKYTVWEHNLRSLQYATEQNWSLEVRLAALLHDV
                     AKPRVKQGDGPDSTFYNHEALGSRMARDLMARLKFPRKQIELISDLIRYHMFYYNVDE
                     VTESSVRRLIRKVGAEHMQDLLYLRMADRIGSGVPKAQPYRLRHWQYMIEKVSQDPIS
                     PGMLAVNGNDIMKLGNIEPGPKIGQVLDVLLGEVLEDPKKNEKDYLEQRVKEFLSLPE
                     KELQELAKKARQERDQVETQRDETTKQKYWVK"
     gene            15337..15411
                     /locus_tag="VFE94_02975"
     tRNA            15337..15411
                     /locus_tag="VFE94_02975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15370..15372,aa:Arg,seq:tcg)
     gene            15448..15810
                     /locus_tag="VFE94_02980"
     CDS             15448..15810
                     /locus_tag="VFE94_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50090.1"
                     /translation="MSIENPFSNPTPPQEEAAKPEKRESGEDLKENDLIIADDQLELA
                     KDILEESHIPHEDKEHKKREEVRGEVDYDVPGEFVVSINNPERPDEPADFEFTKQVFQ
                     LFKDSEIAVRYGKNPERG"
     gene            15956..17188
                     /gene="nusA"
                     /locus_tag="VFE94_02985"
     CDS             15956..17188
                     /gene="nusA"
                     /locus_tag="VFE94_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZX50091.1"
                     /translation="MIDFKNFALLLESIAEEKGLSKDRVIETVEQAIAAAYKKEFGKR
                     GQNIKASLNIQTGNIEFWQLKEVVDQDMIYSEEELEAMKSGEEDASSEAPAKEEEAEG
                     EKKVRFNPERHIMLDEAKKIQKGAKVGDEIELALETKTEYGRIAAQTAKQVILQKIRE
                     AERDSVLEEFQSKEEELVSGIVQRVEGRIVFVDLGKTLGVLPAEEQVPGEFYRLGQRL
                     KFYLLRVEDSPKGPMIFLSRAYPKFISKLFELEVPEIAAGTVEIKGITREAGSRSKIA
                     VASHEDGVDPVGSMVGQRGTRVSAVINELGGEKIDIIEWAEDPEFYIANALSPAKVLE
                     VEVQPKNRAIAIVAEDQLSLAIGRDGQNVRLAAKLTGWKIDVRTPEGRQTGASEEADE
                     EESDAKEEVSSETPAKEE"
     gene            17192..18217
                     /locus_tag="VFE94_02990"
     CDS             17192..18217
                     /locus_tag="VFE94_02990"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZX50092.1"
                     /translation="MKYSQKDLGKSQIEIDVELATEEFEAYRPKAVAELAQSLRVEGF
                     RPGHVPAAIAARELAETNVLEEMARQAISETYRTIVQEQNLPVIGDPTVEIRKIAANN
                     PFEFRVKVAVVPEVQLPDYKESAGQVARKQVQVEEKEIDEALTWLQQSRKAQDSSTLE
                     LSDEFAQSLGNFANLAALREGVKEGLQQEKEMKETERLRQEIVEKIAEKSQVAVPEVL
                     VEREKAVLLDNVKRGVAEVMQMEFAEYLQKSGKSEQELLDSFAKEAEQRVKRFLVLRE
                     VAKKEGIEPTKEEVEQEANQILNHYASAKKAQKEIDPVRLKEYTEGVIRHEKTLQFLE
                     QFASPNV"
     gene            18214..18795
                     /gene="clpP"
                     /locus_tag="VFE94_02995"
     CDS             18214..18795
                     /gene="clpP"
                     /locus_tag="VFE94_02995"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZX50093.1"
                     /translation="MNLIPTVIEKSQYGERAYDIYSRLLKERIIFLGAPINDMVANSV
                     IAQMLFLASKDPKKDIQLYINTPGGLVTSGLAIYDTMQFVKCPISTVCVGMAASMGAV
                     LLAAGEKGKRFALPNAQIMMHQPLGGATGQAVEIEITAKQIVKIKERLNKILASHTGQ
                     SYEKVEKDTDRDFYLTADEATEYGLIDKVIREK"
     gene            18875..20407
                     /gene="rny"
                     /locus_tag="VFE94_03000"
     CDS             18875..20407
                     /gene="rny"
                     /locus_tag="VFE94_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZX50094.1"
                     /translation="MTEQLILLVAGVISLATGVIVGYYARQSIAKRRAGTVEQKLQQK
                     VNEAKEQADQIIVAAEQKAKEVAEGAKSEEEQRRRGFLKTEQVFLQREETLTKRISDF
                     EEKEDTFRKKVEKLQSVEKDLETAQEEVSKKLESVAGLSREDAKKQIFDRVEKEYEQD
                     IVGRIRKLESEGQERFERRAKEIVAYAIQKTAVSQTQEITTTTVVLPSEDLKGKIIGK
                     EGRNIRSLERLAGVEIVVDETPEAVVISGFDPLRRHIAKVALERLIKDGRIHPARVEE
                     EVAKVQAEIDGQVKEAGEAAAYDLGIVGLDPKLIQLLGRLKFRTSYGQNVLLHSIEVA
                     YLASALAAEVGADVTVAKKAGLFHDIGKALDHQVEGSHVEIGMRVLEKFGAEKEVITA
                     MKSHHEDYPYESIESILVQAADAISASRPGARKDTLENYLKRIGELESLANSFEGVEK
                     VYAIQAGREIRVFVHPEDIDDTGAQKLARDIANRIEEELHYPGEIKVTLVRENRFVEY
                     AR"
     gene            20476..21696
                     /locus_tag="VFE94_03005"
     CDS             20476..21696
                     /locus_tag="VFE94_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZX50095.1"
                     /translation="MEEAIQHGAGVTTFLWIAVILLAAKLASLVEKFGQPSVLGELVM
                     GVILGNLVLLGINVFEPIKHDSIIAFLAELGVVILLFQIGLESNIAKMRKVGVRAFLV
                     ATIGVVVPFVLGTYVVGPWLLPGLEQNAYLFLGAALTATSVGITARVFQDLGKLHISS
                     AQIVLGAAVIDDVIGLIILAVVSALVTAGAVSLGVISLIVAKAIFFLVGAIVVGHFAA
                     PFLGKMLSKINTGVGMKFALALSFGLVFAYLAQAQFGLAPIVGAFAAGLVLDPVHFRF
                     FKEERSVEDLIEPVAFFLVPVFFIVTGMSVDLTTLFKPQILLVALGITAVAFVGKIVS
                     GLAAGRGDRLLVGFGMIPRGEVGLIFATIGASLGVVSPDVFSVIVIMVILTTLLTPPI
                     LTFLLKRQPAAAVA"
     gene            complement(21954..22235)
                     /locus_tag="VFE94_03010"
     CDS             complement(21954..22235)
                     /locus_tag="VFE94_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50096.1"
                     /translation="MAGLQTLVGSSVAIIRRIEGEIADAYIATLQGVDENRNVSATNV
                     FDQKTEKSLISGTFYLGFDPPFSTIEIMRLHPPHPRAWWPRRKGAGYHF"
     gene            complement(22346..22777)
                     /locus_tag="VFE94_03015"
     CDS             complement(22346..22777)
                     /locus_tag="VFE94_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50097.1"
                     /translation="MRYTNPFFILIVELAVLWFVSYFIAANWAIPALNESGDTVLFVG
                     ITFVQIFILNVTSYLILRFIYSKTKGLTPSKYHPRRIALFILAAFLPVLLAALAFLYV
                     YSTPELVITIGNVKAAPFLLLLPLPLFVLHLGVAKLLANRK"
     gene            complement(22794..24821)
                     /locus_tag="VFE94_03020"
     CDS             complement(22794..24821)
                     /locus_tag="VFE94_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50098.1"
                     /translation="MRLFFVLFSAAVSLVIFPSSLTNAQAVAVFEDADASVETSVLAA
                     SISPVESVFASFGNGIGVQSLLIQPTISPRENDNVSWGNADATLQATTAPPIFPPAVN
                     FEELLAEKFKPILYFHEEERFRPQEIQVVLDTADIVNTENHIVEETSPLAPTDLSSRT
                     ESHYKLNLDGNTFIEHIPFTDIPYKNTAYAHILEDEGHIVLQYWFFYYYNSWEGGPDH
                     EGDWEFIQLIFEQNKSIEEVVKQSIWPERSAYSAHLGGYRSNWDNVEKENDRPVVYVA
                     EGSHANHFQRGVCDVTIPTGIGSDRGTSAKKLGTLDLRIVIDSILGGPSISTTPQKWL
                     LFGGRWGEDDVPFVKESPRGPLQQPERWPTPISWTNLNTITWDDFYNDSCDGDFDGIW
                     NNVDTQPNDPSNDFDAPGPLGRNTSGTITNRGDRDIKIRDAAVGGAHITADFGSERAT
                     IKFDTLKCITLGTLPVIGPFLSIPKFVPFIGTTTCGSMEVAAEEGSAELQITVGTLEV
                     VGTVSAGQILKYDVVDDQLVIETSGEGEIPLVINGKSTIVQAGDIFSVHEVQIDIKPG
                     SDTNPMNCEAKGNGVIPVAILTTDAFDATTVDHTTVLFEGASETHKSGKNKAMTRHEE
                     DVDKDGDVDLVSHFRQNETTLTCESTEATLFGKTFSGNIFVGTDSIDSKDK"
     gene            complement(24834..25718)
                     /locus_tag="VFE94_03025"
     CDS             complement(24834..25718)
                     /locus_tag="VFE94_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50099.1"
                     /translation="MIHKISLPLAGAVLLFLVAVVFAFYSFAAFQKEGEIPSLFGIPL
                     IRPSLAQSGSPGLPDSQAGLSSYVQRDPSLIDFATISPIFDETIQNGDNYIIGAFNIS
                     RTEIGGTPAPISIHVRVYVDKDGFIIAYLTKDKLAADMFRWHLLDPIINPLDTVLSDA
                     LVAVTTTVGAPAPTDASWYHWNYPSATHFSAAAKRGVGNMYMTIPAGAVFSENPSYSW
                     NGNKQSGSSSTSGTLTLHSIDGTQTQLEPPLGNNRFNIQNLPSIDPGPRYTFEIQNGV
                     GGVAANHFLGVAVVYQIP"
     gene            complement(26094..26765)
                     /locus_tag="VFE94_03030"
     CDS             complement(26094..26765)
                     /locus_tag="VFE94_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50100.1"
                     /translation="MIAFRNVFPQKPLLVTMLAGNENLIVHNAHIARDVGVSGVFIVG
                     HNDVSVEDLQGCYLAVQRACPNWWVGIKYPKLDNEQAIQQAAADAVSGVWLNCNINVI
                     AEQPDAQIKQLRAWQKQYGAKDTLLFGELQEPVWLPGVAAYFAARHVDVVLTKEKDRI
                     VVGINEFGLMKRVLDGRPLGVITHTPENVLPYADCVIVPVLAKRPFVKLPPYRIHQLA
                     QALAG"
     gene            complement(26867..26940)
                     /locus_tag="VFE94_03035"
     tRNA            complement(26867..26940)
                     /locus_tag="VFE94_03035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26906..26908),aa:Gly,seq:tcc)
     gene            complement(26956..27026)
                     /locus_tag="VFE94_03040"
     tRNA            complement(26956..27026)
                     /locus_tag="VFE94_03040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26992..26994),aa:Pro,seq:tgg)
     gene            27088..28062
                     /locus_tag="VFE94_03045"
     CDS             27088..28062
                     /locus_tag="VFE94_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZX50101.1"
                     /translation="MTFGEFLRKLSIAEECRRYNTSLWQCPQFLFTVMGLLIILSVVF
                     SFILGNRYIRDPEITLLFILSLTGVLFAMAFIITRSFEQLAEANRLKTEFIGIVSHQL
                     RSPLTNLKWGLDFLMTETTQHTQQEEGYFHILKDNVNRMHEMVNDLLTVSRAEQGTLP
                     FQEKEFDFAELVEQMLAEFQSAIQASNIQVKVEGERVPKLYNDPSLVRHILGNLVDNA
                     IRYAWRDHEPIPNGDRKAQIVIAYRGAGDRLRVEVRDNGVGIPRQDQKYIFQKFFRST
                     NAVRRQTEGSGLGLFIVKSLLEKAGGEIGFASEENKGSTFWFTLPFKK"
     gene            28074..28442
                     /locus_tag="VFE94_03050"
     CDS             28074..28442
                     /locus_tag="VFE94_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX50102.1"
                     /translation="MKRILFIEDEAAIHKTFTDVLAREDYEIISALDGEIGLRLAKEK
                     KPDLILLDLILPKMDGFEVLKALKADEETKRIPVIVLTNLEQMEDIQKAIDLGARTYL
                     VKSNYDLKEVVDMMKKAIEE"
     gene            28460..30214
                     /locus_tag="VFE94_03055"
     CDS             28460..30214
                     /locus_tag="VFE94_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZX50103.1"
                     /translation="MNVEPGQLKAFLVDAGLVKEKDFEEAVKKSKKTTKPLADVLLAE
                     GFLSEEELMKTEGYILGIPFVSLTDEKIDPEVLKIIPEAIAKSHNIVAFRKKDSELEV
                     AMLDPDDLGTIDFIKKKSNLKILPRLTTPESIKYVLRQYTKTLQAEFGDIIQKEVTGI
                     KALKEQGGGQEAEGKELERVAEELPVIRIVDTLLKHAILQRASDIHIEPTEKEVVVRY
                     RIDGVLRDAMVLPRQAAAGIVARIKVLSNLKLDEHRLPQDGRFKIETDEYKYSARVSV
                     LPVFDGEKVVMRLLAEGSKAFSLGELGFRGEALELVQRNLKRPVGMLLVTGPTGSGKT
                     TTLYTSMEILNTPEVNISTVEDPIEYRMPRVNQTQINPKVGLTFASGLRSLLRQDPDV
                     LMVGEIRDQETANLAINAALTGHLVLSTLHTTNAAGSIPRLIDMGVEPFLISSTLNLV
                     VAQRLVRKLCEEKEAYQLSEKDLEELARYCNLERVLEALVAEKIIKPKQTLRDIDFFK
                     PKPTADFPDGYSGRLGIYEALPVTESIKELIVQNATSDKIAHQAQSEGMLTMVEDGFV
                     KAAQGLTTLEEVLRVIME"
     gene            30222..31448
                     /locus_tag="VFE94_03060"
     CDS             30222..31448
                     /locus_tag="VFE94_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZX50104.1"
                     /translation="MPQFTYTAITAEGQKVTGTETAESERDLGRILYEKGHILTTVKA
                     GEKGKLELPTLKEAFSFLQRVSLMEKLMFTRNLQVMVAAGIPLPKGLDVLAEQTESSK
                     FRRVIIDVKKRVVEGQTLSLSMEAYPHVFSELFTNMVKVGEESGTLENVLSQLTVQLE
                     RQHELKSKIMGALLYPSVVITAMIGIGVLMLIAVIPKLAETFEELGAELPLTTRVVIG
                     LSRFLASYWFIVFPIVLVLLVLLFRFIQTGTGKRLFGQMLLHAPVFSGIVRKFNAALM
                     TRTISSLIEAGVPLVRGLEITSKVVGNSFFEDSLKEAAVRVGKGSRLSEVLREYRSLY
                     PVIVIQMVEVGEETGETGSILAKLAEFYEEEVTQVTKNLTSIIEPILMLIIGAAVGFF
                     AVSMIQPMYSMLGAIQ"
     gene            31453..32382
                     /locus_tag="VFE94_03065"
     CDS             31453..32382
                     /locus_tag="VFE94_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZX50105.1"
                     /translation="MRGFTLAEILVVVAILGLLVAMSLPVFSIFREESRVTTTAEEVA
                     SILRLAQNRTLASQGDTKHGVYFDNIASPHQYVLFQTNTDYASRNAPEDEVYEISDKL
                     EFSVIDFAGGQEVVFERLTGATNQAGSVTVRVASDPAKVRTVFVESSGNVEIGSSISP
                     GDDGNCTSDTPPGGRVCDTRHVHIEYGPRVIDTLTETITLRFSSPPDPDITHDIVIAS
                     SLSGGQIVWEDDIDVYLEKQHVKIHTHKLNDFGVTEFSIHRASDPIHTKPLLITISGD
                     LPAPNKLIEYDAAGDIVISGTESTSVILPYDIQ"
     gene            32369..32875
                     /locus_tag="VFE94_03070"
     CDS             32369..32875
                     /locus_tag="VFE94_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZX50106.1"
                     /translation="MTSNNKGGSVVELIVVVAIIGGSLAAILGLVALSLVSSGIARQT
                     QEAVFLAQEAMEALRNYRDGVPWDQDDPLNQYDGFEYVNFALNPFHLEKSTDSPPQWK
                     LVSGAETVGIFTREIIMEFAHRDANDDIVPSGTQDPDTLAILVRVSWQERGRTHTVEI
                     ASYLTNWK"
     gene            32878..33393
                     /locus_tag="VFE94_03075"
     CDS             32878..33393
                     /locus_tag="VFE94_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZX50107.1"
                     /translation="MRNTKGFTILEVVVYTGILMIVVTALIGFLLWMVQSNTKTAVLH
                     QVAEAARNAIDEMEKEIREAESLYTPTTSLTQLSLETKKNVPLGETSTYVDFFLCGTR
                     LCVKREGQNAIALTSESLDVTNLQFQEVRTGGVSSLSIIFDVERQNPGNHPEFAASIQ
                     ARAVVSLRAYE"
     gene            33386..34669
                     /locus_tag="VFE94_03080"
     CDS             33386..34669
                     /locus_tag="VFE94_03080"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HZX50108.1"
                     /translation="MNKGFVGLYITIIVLMVTLAVLVSMGFSVVVQQRIAQNTVQSAQ
                     AYYGAEAGVEDALIRLKSGKNVCNPPGSPPCSNSISLWSGPAIVITVSDVVGGARTVT
                     AEGDATNRFRTVQTVFEISSTGVGFNYGAQIGEGGLQMDNNAIVNGNVFSNGNVIGEN
                     GSRITGTVQIAGPGKILDNVDIGTVGIGGDAYVDICQNSGSGTRIEGDLHTNSAGPCV
                     VVGSTTSSGLPIAPIPLPILDSQIDAWKLDADDGFPLVNITVTDTLPGVDLSLGPKKI
                     TGELIVDNNATLEVTGTLWVVGKVTIKNGAVVQLNPGYGTSSGMIISNDIVLLDNNSV
                     SKGSGQAGSYLMYISTSTSSQAIESKNNAKADILYTNTGTIKVDNNAGLREVVGYRMH
                     LLNNAVITYESGLANSAFTSGPGGGWVVTSWKEIE"
     gene            34689..35798
                     /gene="pilM"
                     /locus_tag="VFE94_03085"
     CDS             34689..35798
                     /gene="pilM"
                     /locus_tag="VFE94_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HZX50109.1"
                     /translation="MAGLLTLHPKAFGLDLSDLSLKIVSLEERGKGFRLSSFGEASIP
                     EGVIEGGEIRKEEKLVQVIRKAVAEVKGKKIQTNYMVASLPEEKGFVQVVQFPPMGKE
                     ELGNAIRLQAENYIPYPVDTVYLDFTVIPPSHNHVDHLDVLIASLPRTIVDSYISAFE
                     KAGFTPRALEIDALALSRAVIEKGVAPVPVLLVDVGNARTKFSIFSGYSLRFTTSVPV
                     SANTFTQAVATRLGVEEERAEELKRMYGLDSIEEKLGDEVFQAMAPSATEFVKQIQKY
                     LTYYETHIPHQHLDRPQKKIKKVVLCGGGANLRGLPEFLMRFLKVEVAVGNPWVNILS
                     ASLKELPALPFQDSLRYSTALGLGLRGVKREVSDD"
     gene            35791..36318
                     /locus_tag="VFE94_03090"
     CDS             35791..36318
                     /locus_tag="VFE94_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZX50110.1"
                     /translation="MINLLPPEYRQRLKDEEYFRLVLILGALLLLFFVSLSLMLLSIR
                     IYLSGEIDAQKILVERQQEEYNRENPLELNVKDLNRSVAQLAAFYKEQILLSETLQEV
                     TAALPPQAYLTSFVYNAGAPGKISLAGFAPTSEDLQAFRGTLEQNPRFSNFFFPLSNS
                     TSERDITFSFTFTVQ"
     gene            36321..36848
                     /locus_tag="VFE94_03095"
     CDS             36321..36848
                     /locus_tag="VFE94_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50111.1"
                     /translation="MNTKRNLLLAAALFLGAAFVFVSFVVYPVFRGVVHDHERALEQR
                     REFVQLGEDREHLAEFERLFMREQSAFDQLERVFLDLRSPIEFFRFLDRSAEEFGITL
                     EKTPGLPQTTPEEPWTYVDVHLEGEGTYPGALAFIEKIESSPYLLELKGLNFSKAEGQ
                     LLVRFSINTRIYAKD"
     gene            36835..37170
                     /locus_tag="VFE94_03100"
     CDS             36835..37170
                     /locus_tag="VFE94_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50112.1"
                     /translation="MRKISFPKRFNSKKILAFLRWMGENAFLGFLVLLVIALCISSLV
                     FYRYVFLLESAQLEFRPSQNTFQTKPFEDIVRVWQERGQAFEQASPENYRDAFAPAPL
                     PPEAEESDN"
     gene            37207..37278
                     /locus_tag="VFE94_03105"
     tRNA            37207..37278
                     /locus_tag="VFE94_03105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37240..37242,aa:Arg,seq:tct)
     gene            37295..37368
                     /locus_tag="VFE94_03110"
     tRNA            37295..37368
                     /locus_tag="VFE94_03110"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37329..37331,aa:His,seq:gtg)
     gene            37443..40340
                     /locus_tag="VFE94_03115"
     CDS             37443..40340
                     /locus_tag="VFE94_03115"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZX50113.1"
                     /translation="MLSLLGILIFVFIAVWLHRRISRIEELLESKSAPLLSPAPQQAL
                     SPLVPTQQTQTPISGNEELAVIDKFVVWAKEDWLLKLGAFLLLIGFGWLATYAFANNW
                     IGPMGRITLGLVAGVLILLLGWWRIKTYVYQGSTFLVLGSSVILLTTFAARIVYDFFT
                     PLAALGIMFMSAAFVAFASVIYRHLPLALASLLSAGVAPLLTSPLSFAVSLSTDFVTL
                     FSYLLVVTIGTVWVVALTGWRILTTVALIIVALYSLPHLFIGRSLGVEIVLAFTFAFT
                     AVFFITSILGILKLAKEKIVPDLITAGGTGLFLLVWILMAAPEEWKSILMVAWILVFA
                     FGGFLAFRLTQRIEPFYVYLGVSIAMLAGATAVELQGAALVIAYSIESGVIALVAYAI
                     LRDLRIAQMLSLLLLGPAYLSVGSVISRSWNIGVVHKDFFVLFVLAVIFFGLGLFFLW
                     AQKQKISNEKKESAYFTAGLLIVGSAYMHLLLWLSLHATKLSDDVATMFSLVAYTVIG
                     LATYFYGKTKEKRIMRLYGAVLLTLVVGRLLVIDVWSMALAGRVVTFFLVGILLTTTA
                     FVGRSKKALPMLVLATAGAMTFYASSAEALSTASQETISAYRQLKELQPVAILVPTVV
                     EIPFESQELERFSFAVLDEATDTFQPVFFKQETSVNEIPITTINATNQSALNAGRMVD
                     GNVQTYTAFALPEEAQGSTQLILQSTRPITSSSLTLLLDNHVALPTSIEIRAQVSGTN
                     TIVLAKIPMRQATIQFPRTTSATWTIALTYAQPLRISELKLAQENATRSSSHALRFLA
                     QPGRAYQVYFNPDRYVSPPVGEAGNLADNQGVLRLPSPLSQTNVYYKQADVDLDNIPD
                     ILDNCVSVRNTDQADVNRNGRGDACDDFDRDGIVNSQDNCPNDPNRGQADVDADGIGD
                     VCDTEESRFTERYKWIPWLGIAFAALTLIVLFALTARSTLQKRNQDKPPTV"
     gene            complement(40420..40557)
                     /locus_tag="VFE94_03120"
     CDS             complement(40420..40557)
                     /locus_tag="VFE94_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50114.1"
                     /translation="MIFDILSPLVSILDDDGMTDDDGMMDDNTEEDTDDDTDVEEEED
                     E"
     gene            40698..40769
                     /locus_tag="VFE94_03125"
     tRNA            40698..40769
                     /locus_tag="VFE94_03125"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40731..40733,aa:Arg,seq:cct)
     gene            40799..40869
                     /locus_tag="VFE94_03130"
     tRNA            40799..40869
                     /locus_tag="VFE94_03130"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40831..40833,aa:Ala,seq:ggc)
     gene            40874..40958
                     /locus_tag="VFE94_03135"
     tRNA            40874..40958
                     /locus_tag="VFE94_03135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40908..40910,aa:Ser,seq:cga)
     gene            40967..41359
                     /locus_tag="VFE94_03140"
     CDS             40967..41359
                     /locus_tag="VFE94_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HZX50115.1"
                     /translation="MALFKKQQPEEQTPQALRKKVQALEKRLEQTAKELEQLKQQTRQ
                     AVTRVGIVRFNPFAEMGGDQSFAVALLNEEHTGFVLTSYYGKDLNRVFAKPVQKGSSR
                     YELSDEEREALEKAMGVEKPKSKRRKKK"
     gene            41364..42875
                     /gene="infB"
                     /locus_tag="VFE94_03145"
     CDS             41364..42875
                     /gene="infB"
                     /locus_tag="VFE94_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZX50116.1"
                     /translation="MITQNAENRIAKEHTGARAPVVVVLGHVDHGKSSLLEAIREDFR
                     ITAKESGGITQHMGAYQAQYQGKNITFIDTPGHELFSAMRSRGAKVADIAVLVVAADE
                     SVKPQTVEAIEQVKKAGIPMVVAINKIDKLQANSQRVKQELAQHEVIVESYGGNVPSV
                     ETSATTKQGISELLEMLLLVADLESLKANPEQPAEGVVIESSLDPKRGPLASLLVLNG
                     TLRLGDWLSTPSSSGKARMIEDFQRQQIAEAGPATPAVVLGFLIPPRVGDVFRAFESE
                     TAVQEFVWENELKEVAKGASEVNPEAKLYNIILKADVAGSLEALEDVLHNLPQGEVQL
                     RLVEAGVGEITENDVKLARSTTAVVFGFRTKVSVQAADLAEKEQISIETFQIIYELIQ
                     RVREAMEQSLKDEGGRQELGTLNVLAIFLVDKTRQVIGGKVVEGEVRKGVRAEIIRAG
                     EVVGQGKVTNVQHDKKDVPSVAKGKECGLSFEAAEKVQAGDQLQFFVVRAPKK"
     gene            42877..43218
                     /gene="rbfA"
                     /locus_tag="VFE94_03150"
     CDS             42877..43218
                     /gene="rbfA"
                     /locus_tag="VFE94_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZX50117.1"
                     /translation="MKNRIAQVEELMRHELANIILREVEMPEGAVVTLTRVVAAGNLQ
                     QARVYMSVVPDEKGGEALKVLSQNIYEIQQILNGRLKMRPVPKIQWVLETATAEAQHI
                     EELLDKIKKQG"
     gene            43230..43304
                     /locus_tag="VFE94_03155"
     tRNA            43230..43304
                     /locus_tag="VFE94_03155"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43262..43264,aa:Cys,seq:gca)
     gene            43307..43388
                     /locus_tag="VFE94_03160"
     tRNA            43307..43388
                     /locus_tag="VFE94_03160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43341..43343,aa:Leu,seq:taa)
BASE COUNT        11052 a        10102 c        11151 g        11291 t
ORIGIN      
        1 ctgaaagttc cctgtttgcc acgacccagg tgccggcccg gtaatggtta cgtctggcgg
       61 aggcggaggt gctatgcaaa cagaaccaaa gttgccgacc ccgtcgaccg cctgccccgt
      121 gccggcaaga acttctgtgc cattataata caaggaatag tttatactat gaactgggcg
      181 aatatttgat ggatttgtag aagcgcatgg gcttaaggtt ccccaacatg cccgattagc
      241 agggttcgct tcaagtccaa gacctgaccc aggatcgccg tttatgtgaa tccagcgtat
      301 ggctgaaccg ggcgtaccct caagcactcc cttaagcccg ctatcagtaa ccacatctcc
      361 aacatgagcg cttgcggcgc ataaagcgtc tgcgtttacg aaaggaacgg ctccattaca
      421 accatgtact tccgccccgt ttggaagtgt tcctaggaat tgctctgtgg tgccgtcggc
      481 gcagccgctg gccggctggg cgtggacttc tgctggctga atagaaacaa aaatctgcag
      541 ggcgagcaaa gatacactgc atacgacaat acctgcaacc agaaatcgaa acattatact
      601 attataattt atccgcattg aatctgccac caggaactgg tgccggtcca tatagtaacg
      661 ccaagaaggt cgagcgcaaa gtcaacggca acccaagccc cgtaaataat aagcaatgca
      721 ataaccgtgg taaaaataat tcctctgccc ctgtttaaaa gagcggggtt gcccaggttt
      781 cctataatta tactaaatcc acccaccacg atcataagcg cgcccaaaag catcacaata
      841 gcaaaaccat tattgaaact cgaggggaag agcaaaaaag taataatgtt gtgtacgagc
      901 gcaaatccgt ggcacagggt gcatggctgg ctttcgtcga gtcctgtggt gagggggtcg
      961 tctgaatccc tgccgcacgg caccagcccg cccgtaaacg cgctgttgta gcgcagtatg
     1021 gcctcgctaa agttgccggc caggtctgta gcttgcgcgc gaataaagca tttgcttccc
     1081 ggcgagcacc agccgggaat tagtcccatg tcggttagcc ctgtgccgca agcagaaggc
     1141 cctgtgttat ggtcggtcca cgttcctcct ccgtcgtttg agctggaaag gctgcacgtg
     1201 tctataaacg cgtctgtggc gttgtactta ataggaaagt ctgccgccag gtctgatcca
     1261 tctgcgggcg tcggcggtac gtcaaaactt gccacgggtt tggtatagtc tatggaaaaa
     1321 tcccgttcgt cttgaacaga cggcactgtt gattctaccc agcgaataat aacttgtccg
     1381 tttccagaac gtacgccctg ttggtgagtg gtagatcctg tgatttcata ggctgggtct
     1441 acatagctgg aacctccgcc cccgccagca ccaccgacag cagcattggt aggagtaccg
     1501 ccaccctggc ctcctcctcc tccgtaatac ccaccgcctc cgctgccgcc agaaaatgag
     1561 tcaccctgtc ctcccgtgcc aaaacttccg cttgttgcag atcctcctcc tgcgctgcct
     1621 ccctgattat ttatagtatc tccgcccccg cctccgatcg aaggcgaggg agcgggtcta
     1681 cttctagctt gataccaacc aacattatag tctaccgaac cgttgcctcc tttaccgccc
     1741 ataagtccac ctccgctgcc gccacttcct ccgctggcta aatgaacgtc gtcaaattca
     1801 acaaacccgt tgcctccttc tcctcctccg cccgcgacag caatacggct tgcccctatt
     1861 cttatatcag aagcgcctcc gcctcctcct ggtccgcctc cgttaaaacc tgaagttccg
     1921 tttcccccca cgaagatttg aagcacctgt cctggcgtaa ggttttgtaa acgagtgata
     1981 acatatcctc ctcgacctcc gcttcctccg cttgctggta ggcccgagta aaacttatca
     2041 ataccccttt ccccctgggc gccccacagc tcaacttcta tttctgaaac tcctggtggc
     2101 acaggccagg tttgtacacc tcctgtatag gcaaaggttt gcgaaattgg agtgctgggc
     2161 ggctttccca caatccttac attgcaggcg ttttgccctt cctggatgca ccagccggaa
     2221 gcgccgacct gaactgttgc cgattgatta gctccgcaag agactgtgcc tcggttttgc
     2281 cagccaacac caccgtcgtt tgtttcgatt tcacatacac cagaaacagt tggctgccac
     2341 gtaatgacta cctggccgtt atctgtttgg tatccttggg tatgtgttgg gttaagacac
     2401 tttggtggtt cgcagtagct tgagcctccc cctcctccac ttcctccttt gtcgtcagaa
     2461 cttccgtctt cattggtacc tcctccaccg cctccaccgc cgtaccagcc tcctcctccg
     2521 ctgccgccat ggtaaaaccg aaatccagca ttgaatctgc cacccccgcc accttgccca
     2581 aacgaaccag gggtgcccgc gaagccatta ggccaaccta aacctccccc acttagttgg
     2641 gtgccgcctt ctccgccagt accgccccat gtgccaacac caaaaccgcc actaccagga
     2701 aggccattgg cacccacaag atcgccgccg gcaccaccac ccccgcccac ggaagaacca
     2761 gggccgctac cccgaccacc tccgcctcct gctaccacaa gccgactgtt aagcccggca
     2821 ggatcatccc atggtccaga aacggtgcgt atgtcagagg cacctccacc tccttgaccc
     2881 ggattggatg cacctccgaa ccctccgccg ttaaagcctc cagttggtcc tgctccagcg
     2941 cctcctacat agatatttaa ggtctgcccg ctcggcacgg gaatggttgc ctgcactcgg
     3001 ccgccaagcc cacctgttga gctgccgcct tgcgcccctc gcacatccac cgtaatttcc
     3061 gtaacgccgg agggaacagt ccaaggttga gaatctccgg tatagtcgaa tgtttggctt
     3121 tgaggaggag aagtaggaaa ggtggcgtcg tactgcacct gaaaatcccc gttttgccat
     3181 gaactaggcg ccggcccggt aatggttaca gacggcgggg gtggaggtgt aatacaaata
     3241 gcgccgtcga gtggagagcc tgaagggtaa gcattgcccc tgtttccaag gacatttgtt
     3301 ccgttgtaat agagagagta gagggaacta agaataccgt gcgcgggacg aaaggtcgca
     3361 attggtccaa atgccgaaga actcaacata gtacaaggag cttgtgtccc ccagcacaag
     3421 ccgactgctg gttctggacc ccccaacaag ggcccagggt caccgttcat atgaatccaa
     3481 cggcgttctg aaccggtcgt actctcgagt gcgcttttaa gtccgttgtc taaaaccacg
     3541 tcgcccacag tcgcgcttgg ggcgcaaaga gagtctgcgt ttgcaaaagt tacacttccg
     3601 ttacatccat gaactgttct gccgtcgggg agtgtgctaa aattctgctc ggtggtgccg
     3661 tcggcacagc cgctggcggg ttgggcgtgg acttctgctg gctggagcga aacaaaaact
     3721 tgcagcgaga gaagagttac gccgcataat accgttgcta aaacgagaaa ttgaaaccct
     3781 gtgtttatcg cggggcgatg cattactata ttataaatcg tttgcgaggt ttatccaatc
     3841 ctgcacagac aatgtttccg cgcggcgggc ggggtcaatg ctgttttttc gcaagatagt
     3901 ttccatgtgt tcccttggac gtttcaggcc ggcagacagg ttgcctaaca gctgttttct
     3961 ggggtgggtg aaccccgctt ttaccacgga gaaaaacttt tgcgcgtcaa tatccggttt
     4021 ttctttgcgc gggacaatat ggataatggc agaatccaca tggggctggg gccaaaacga
     4081 tgttttctta atatatgaaa taattttggg cgtggcgtaa aattgcaccg aaaccgccaa
     4141 tatgctcatg tccgggggtt tcgcgcaaat tcgttgcgca acctcttttt ggattataag
     4201 cgttatggac tgcggccggt tttccgcctc gaggaatttt ctgatggtcg gagcactaat
     4261 atagtatggc aagttagcaa ctaccttgta tgtttgcttt ggaacctgag ttttgagagc
     4321 gtccccatga atgacttcca cgtttttgaa ctctgccagt gtccccttga ggatttccac
     4381 cattttcgcg tctttttcaa cggccaccac tttttttgcg tgctttgcca attcctgggt
     4441 aagggtgcca atgcccggcc ccacttccaa aaccgtgtct tgcggctgaa tgctggcggc
     4501 aacaaccatt ttttggacta tgtggtcctc gacaaggaaa ttctggccca agccgcggct
     4561 tggctttatg gtatgtttct gcagcacggt ttttatagtt ttggaggaag caaaattcat
     4621 tggtatttgg tatattgtac cctgtggata caacgcttga ccagggcctt tgagaagagt
     4681 ataaaggata gtgcctatga gaaaagagcg agaagagcct tcaagttcga ttaatcgtag
     4741 agcgcgcaaa ccctttttca ccgtagctct tggagctatt tttttgttgg ctctggcagg
     4801 tttgggagcc acggtattct ctgattcttc tggggtggga gcgcgttttt cggctccctt
     4861 gccctcaacg tctgcaatgc acctgtatgc gaaccaggca gacgtagcag aaaccccgga
     4921 attcgtaaca gtaggagaaa caggagtttt agctaccgct ccgcctgttg cggtaagccc
     4981 caaggttctg ggagctatta ttggcgaagc gaatttcgcc cagcaaaaca gaagggagct
     5041 tcttcattat gccgtggaag agggcgatac gataaaaagc ctttcagaac ggttcggcat
     5101 ttcgcaaaac actattcttt gggtaaacga gctttcgagc gtttctccgc ttactctggg
     5161 ccaggaactg gttattttgc cggtatctgg cgctctgcac ttagtgcggc cagcagacac
     5221 gttaagcgag attgccttat ggtaccaggg agatgccgag caaatcatgg aggcaaacgg
     5281 gatagcttcc gccaaagata tctttgtggg ggacttcttg ataattcccg gggggattat
     5341 gccaaaaagc ctgcccgtag ggcgtttaac tcctttggca aattcctact ttatttatcc
     5401 ggtatctcgc tcgcacaaat taagccaggg gctgcatgcc tttaacgcag ttgacctaag
     5461 caacggccag tgcaacgaac ctgtgtattc tgccgcgggc ggaaccgtgc agaaagtggg
     5521 ctatcacagc gtggcgggaa accatgtgcg ggttttgcac cccaacggag tggtaacctt
     5581 ttacggccat ctttcgggcc aaacagtgga ggtaggacaa aaagtttccc agggcgcaat
     5641 agtgggctat accggccaca caggctatac tattcctgcc ggtcctgacg gatgccacct
     5701 gcactttgag gtgcgcggcg catcaaatcc ctttgtgaaa taaaaaaacg gggcagaggc
     5761 cctgtttttt tatgtgctta cttatttgca cttgccgcag gtggcagctt ccggagaaat
     5821 ttggaggcgt tctgttgcaa tagggttgcc gcagttttgg caagtgcccc aagtgccctt
     5881 ttctatacgg tctaaggcgt cgtttatgtc tttgagcttg gtttccaagc tgtgttccaa
     5941 gtgtagccgg gtggaatatt cttctacttc atctgctgct tcctcgaggt tgcttccggg
     6001 tattctggga tatttggagt cccagtcgcc ttttacgttg gggtctttgg ttgcaaaaga
     6061 agcgagctcc gattctaagc gttccttttc tttttcaagg cgggtcttta gttccccgat
     6121 atctgttgtt tccataaagt tttcctattt attttgtgaa agatgattga attgcagagg
     6181 ttcttctgaa gggaagaaaa actttttctt gaggtcgagt acgcggtttg cagaagcctc
     6241 aatttgtttg cgcagtgttt cgtcggttgc agcctgttgc aaaagcagtt tgtgcgcctg
     6301 ggtggcctct ggggtctctg caaacattag tatattcatt cctgcacgta aaggcaagaa
     6361 aacgatttct tcaaaggaat agttgtcaag aagggcgggc tgtgcaaggt cgtcagaaag
     6421 gataatgcct tgaaacccga gctcttctcg aatgagagac atgccttcta tagagaagct
     6481 aaatgggtag tttttgtcca cggtagagta aataacgttg gaagtaagaa gaaattccgg
     6541 ttcagaggaa agcgcaaatg cgaaaggaga aatgtcggga aactcctgta cggtagcaag
     6601 tttcttctct gggttaaaag aaatgctgcc gtatcccggg aaatgcttga gcgtagagag
     6661 gatattctct gatttttggc ccgctaaaag cgcgcgagcc aaacgcccgg cttccaaacc
     6721 ctcggtttga aaggtgcgct caaacaaaaa atcatgtggc cccgtggtgt ccagcacagg
     6781 agcaaggttg aggttcacgc caagactttt gagttcgcga gcgcgttcct tgcccaccgc
     6841 gtaggcttga gcaggggttt caatagtaga ttgggcggtt ttttcctttc caaactgaat
     6901 tcttgagata agtcctcctt cttgatctac cgcgacaagc agaggaagag agctgtattc
     6961 gagagataac gcctgcagct cttgcgtaag tttttggagt tgttgggagt ctgtaatgtt
     7021 tcttgctaaa aggagaacgc cccctggtcg gagacgtttc atgagttctt caagttctgg
     7081 ggtaaggtac gtgccttcga atcctatgag taatacctgg cctattgctt gctccaaaga
     7141 aaggttttga ggaggtttaa aaaggcctgg actgttttct actgcgagta tctgattttg
     7201 caataaggca cccggagaat tttgcagctc gtcaatagct tcttttatcg cctcaaaaga
     7261 agaagcgaga ataaagcggt tgttcactat tgtgtacaca atgcctacgt cctgcgtgga
     7321 gaacgtctga aaacgaacct ctaccccgtt atacgacgtg ctgcggaaag attttgtata
     7381 tccgtttccc ggatttcccc aaaagtcgcg taaggggacg aaagcttgtt ccatttgagg
     7441 ctcccatgcg cgcaaacttt gccagagcgc cgtagggtct gcaacttccg agactatgcc
     7501 aaagcggtta cctctttcat aagagtagag gaagaaaaac gaggtttgtt gaagttggtt
     7561 gcttactgct tcgggaaccg gaacttcaaa aattccaaag aaagagttcg cgctctccag
     7621 tgatgtattg tcttgtctgt tgcgaaagac gagcttggta aaaccctcct gttgatctgc
     7681 ctccaacgat tgtttgaggg tactggtaag gtctgccttt tctgaaaaca gaagggtaga
     7741 agtatgggca acaccaagag gatcagaagg agtgggcgcg ggttgcgctt gaggaggggg
     7801 ttgaggggaa gggccgctgg ctggcggagg tgtgggcgtt ggggtggttt gctcttgaga
     7861 gaaaaactgg gaaaggaaag gaatttccac cgattgattt tggaaaacaa accagtagag
     7921 aaataggccg atattgagaa cgatgaacgc aaaaataatg actatgatag cccgaaccaa
     7981 aactctttta cttcgggaag ggggcgtggg aagagcttca aacctgtttt cctcgccagg
     8041 aggcaaaatg gagctttgga tgttgcgcat tcgttcttcc tttcttgttt cttcccgctg
     8101 ctttgccgcc aaagctgccg atctgatttt cgccacggcg tctttctctc gctgggcgtt
     8161 ttgttgcgct ttcagttgcg cgatgcgctg ttgttctttt tttgcctctt cctcgcgaag
     8221 ccgggcaatg tcttttgcca tggtgcgtac ttcgccgcgt tccagatatt cgcgtttttg
     8281 ttccggccgg aagggctctt gttgcctggg cggcattggt ttttctggag gctgaatcgg
     8341 tggtgcaggg ctgccagatg gcgtaggagt tggccgttct ggctgcgatg gccggggaaa
     8401 aggaggggga gaaactggcc tcgatggtgg tgccggagtt ggcctggggg attctggaga
     8461 aggctctatt gggatattgg ggggaggagg ttgcttgcga acaaaattgt cttcttgcat
     8521 tctggtattt tctcatttcc aagaaacatt taccagcaat gcacctgtgg aaaactttta
     8581 ctgctccttt gcctccttga gagaaaggtt aatccttccc tgtgcgtcga tttcgattac
     8641 ttttacggga atgatgtcgc cgagctttac aatatcctgt accttgttta cgcggtaggg
     8701 agctaactgt gaaatatgca caagtccttc ttggcctcct ccaacctcca caaacgcgcc
     8761 gaattccata atcctcttta ccttgccctg aaaaatttct cctacttgca cttcccgggt
     8821 aaggtttctc acggtttcca gggcctgttc cagttgtgcc gcatcctgcc ccgttacaaa
     8881 aacaaggccg gtttcttcaa tgtcgatttg cacgtcgaat tcatcgataa ttttgttaat
     8941 cgttttgccc ccagggccga taagaagccc tattttttct gggttgattt gcaaagtggc
     9001 aatcttggga gcccagggag aaagctcttt tctgggttcg gcaagtattt tggcaataac
     9061 ttcgtctaaa atatgggaat gggctttctt ggctttggct aatgtttctt tgaatatgtg
     9121 ttcggtaatt ccccggactt ttacgtccag ctgaattgcg gtaacacctt gtttggaacc
     9181 cgccacttta aaatccatgt cgccgtgatg atcttccggg ccctgaatgt cggttaacag
     9241 tttatattcc ccgttttgtc ctagcatgag ccccatggaa attcctgcca cgggccgttt
     9301 aatgggaact ccggcatcca ttaaggcaag cgtggaagag catacggaag ccatagaggt
     9361 agagccgttg gaagatacca cttctgttac aatacgaatg gtgtaaggaa attcttccgg
     9421 ggttgggata acgggaaaca agcctttttc tgcgagcatg ccatgtccta tctctcgtcg
     9481 gccagggcct cggagcattt ttacctctcc tggcgcataa gggggaaagt tgtagtggtg
     9541 cataaaacgc tttttgccaa tgaactccat gccttccaaa aggcgttggt cgccgggtgc
     9601 gccaagcgtc aaaatagaaa gggttctggt ttgcccccgt tcaaaaagac cagaaccatg
     9661 cgttctggga aggaacccgg ccaaagcaga taatggtctg atctcgtcga gtttccggtc
     9721 gtccacgcgc tgatttcttt ccagggctcc cttgtggaca atgcgatcgg tttcttcgtc
     9781 gaaaaaggaa tcaacatgcg aaagaacttc ggcgttttcg tacttttctt tggtaaaagc
     9841 cgtaaactct ttttttactt cgtccaccgc tgccattctg gcctgcttat ctccctgaaa
     9901 aagagcgtgt tcgagtttgg gttcgagcca attttggagt tccttgcgca ctgtttcttc
     9961 tataggcgag cgttcctcgt gcgcgatttt ttctttgcca agcttcccga taaggtcttc
    10021 ttgaaacgca ataagggctt ccaaatgcgg cttggcaaat gcaagcgcct gctcatatac
    10081 ttcttctgac acttcatcgg actcggcctc gatcatgttg aacaggagtt cctgcccttc
    10141 ttttactgcg gccagcacga aatcaaggtc gctttgtccg cgctcttcgt aagtggggtt
    10201 taccacaaac tggccgttta ttcttcctac gcgcatccca gccaaaggcc ctttccaggg
    10261 aatgtcggaa agggaaagcg caatagaggc gcccaaaagc cctgcgacat caggatcgtt
    10321 ttgtgcgtcc caggaaaggc aggtggtaat tatctgtact tctcgtttga agtttttggg
    10381 gaacaagggg cgaatcgcgc ggtcaataag tcgggcgcaa attatggctt cgtcggaagg
    10441 gcgtccctcg cggcgaataa agcgagaacc caagattctg cctgcggcat aaaaccgttc
    10501 ttcgtagtca acaataagag gaaaaaatcc ctggttggaa gtttcagagc gcgacataac
    10561 ggcagtaacg taggtgagcg tttctcccgt ccggataagt acgttcccgt ttgcctgctc
    10621 cgcccagttg gggaattcaa tggtaatttc tttctctcca agctttacgg tgtatttgtt
    10681 catcgtgtaa ttttaagagg ccaacgagac ttttttaaca gatatttatc ttcctcttct
    10741 tccaagtcaa cgaattcatc ggccacctcc cgaagccttg aagaagttga ttttccaaat
    10801 gccataactt ccacgcgttt tccgtggttt ttgaggtatt ctacaagagg cacatagtcg
    10861 ccgtcgcccg ataccagcac taccgcgtct gcgctgtctg cggttttaat ggcgtcgaca
    10921 gcaatgccaa cgtcccagtc cgcctttttg aggcctccgt aatactcctg aagatctttt
    10981 acgcgagttt caatgcctac gctcgtgagg gcgtcgaaaa acggcttttc ttctccggtt
    11041 ttcgtgcgca ctacataagc aaacgcacgg ataagttttc tgcctgcaac cgcgtttttt
    11101 acgatttcgc ggaagtttac gcgggcctgg tgaaggtttt ttgccgagtg gtacaggttt
    11161 tgaacatcga taaaaacagt aacccgctga tctttatttt tgaggctcat gtcgaaatgg
    11221 ccttattttt ttaggccggt ttttttcaaa agagatttat atcgtttttc gtcctcccgt
    11281 ttgaggtgcg ctaaaagagt gcggcgcctg gccaccatct ttaaaagacc tcgtttggag
    11341 tgaaaatcct tggcgtgctt ttttaaatgg gaaagcaact tccggatttc ttcagacaaa
    11401 atcccaactt gcacttcagg agagcctgaa tcgatagcgt gagttttgta tttctctatg
    11461 attttttgct tgtctttggt ttgaagcatt aggcgtatta tagcagaaaa gcaaaaattt
    11521 gcaagtgggt gcccccagaa ggaatcgaac cctcatcaac ggattaagag tccgcagctt
    11581 taccattaag ctatgggggc ttttggcgag gtttattata taaggaaaat cgctactttg
    11641 caaccttttc tatggttctc ttgcagcttt taatgccttg gcgtaccaca ggagttcccc
    11701 aaagaatttt tggaggcgtt cggggtaaga ggggtcggtt atgttgtcct gttcgtcgaa
    11761 cagggttttt acgttggaga aatataccgc ggttcgaatg ggcaccattt gcagctctat
    11821 agccacaagc cgcagctttt ctaccatgcg cactccgccc atgccgccac tcgccacgcc
    11881 gcaaacgcct aggggctttc ggttatactc ttcatagagc atatcgagca tcattttgag
    11941 ttcgcctgga tacccgtgat tatactctgg agctacaata ataaggccgt cggccctgct
    12001 cactttttct gaaaaccttt ttgcaggctc ctgctcttcg gtgtcgtcgg tagctgcaat
    12061 tctgtagtct cgaacgtcaa taagctcggt ttccacgcct tcctgctttt ggaattctcg
    12121 caacacaaaa ttcgccgcct tctctgatct tcgaccttct ctcgctgtgc ctaggattat
    12181 gggaatatac atatgattat tacaatttta taagttcaga ttcttttcat ctttgctact
    12241 caacgaaccc aggacagccg taagcgttgc gacctcattt tttgtaatat cccaagggac
    12301 ctcgatcaca aatttttgtg agttgcccct aagaggaagg tcaatccgga ctatggcttg
    12361 agcatttgct atttcgtggt cttcaatact tcctttgcct ttctgaactg cgttggggat
    12421 tgatcgtttt gttcgctgca ccaccctagg tgtccagctc gccattttgg ttgggtcgat
    12481 gccgtatgtt ttataatcct taaggacctt ttggacgcgg gatttgtagg tagccaaaga
    12541 agtatcggtg aagtcctttc ctccatgctt tgagcaaaca cttttgtata tttgttcaat
    12601 attttcttcg aacttatcga cagaattgag ctcgtcttcg ttgagctctt cctggaaaag
    12661 ctgcaaggct cgttttatgc cgtggcctgt gtttttggga tattttcggt ttcggaccgc
    12721 tttatctacg aaggcgatca aatctttaaa attattcatg cgagattgct ccaaaataaa
    12781 ctttttagat gaccttgatg ggatgtactc aatataccgt atcaacccta tcttttcaaa
    12841 tggatacccc aatctcctct ctctagatag aaaaaaacac gcgttgaaac gtgtatcttc
    12901 attttgacga aaatcctagc agtttggttt ctccacactt tctccacacc tataaacctc
    12961 ttaggggtat tcttcagcat aagacttgcc tcgttagaca tttgtggacc tggggagaat
    13021 cgaactcccg actcggcaat gcgaatgccg cgtaatacca ctttactaca ggccctttat
    13081 ttaatttttc gccattcacc gggttttaga ttttcaagct taatgtcgcc tatgcgcgtg
    13141 cgaattaaat gagctatctc ataatggcat gcttctgcca tgcggcgcaa ctggcggttc
    13201 cagccttggt gtatgataac ctccagctcg ttcttgtcga tttgggttac tttgtctgct
    13261 tttgcaatgc cttcggttaa gcgaatacct tttagaaagg cgtttataag ctctttgctt
    13321 acagcctttt taaaaaccag gcgatattct ttttgctttt cgtatttggg gtgggtaagt
    13381 ttgtaaatta agtcgccctt gttgctcaaa acaagcaggc cctctgattc gtagtctaat
    13441 cttccggccg gctttaagtc ttgaaactct ctgggcagga ggctcattac gatttttctt
    13501 ttcttggggt cagatcgggt ggttatgtac cctttgggtt tataaagcgc aacggtaatg
    13561 gattctttct gtttgctttg gagagttttc ccatttgcag agacgcgatc ttttgcaggg
    13621 tctacaagct ggccaagttt tgccaggctt ccattaactt gcactttctt gtctgcaatt
    13681 aatttgtctg ctgcgcggcg ggaaatccca agctgctgcg caagaaatac gttaattctc
    13741 ataagtgtcg gggatgtggg ggttgaaccc accatctcat cgtcccgaac gatgcgtgtt
    13801 gccgctacac taatccccgt ttactttacc caatattttt gtttggttgt ttcgtcgcgt
    13861 tgggtttcga cctggtcgcg ttcttggcgc gcttttttcg caagctcttg cagctctttt
    13921 tctggtaacg aaaggaactc ttttacccgt tgttccaaat aatctttctc gttctttttg
    13981 gggtcttcaa gcacttcgcc caataggaca tcgagaactt gtcctatctt tggccctggc
    14041 tctatgttgc ctaacttcat gatatcgttg ccgttcacag caagcatgcc gggcgaaata
    14101 gggtcttgcg ataccttttc aatcatatac tgccagtggc gaaggcggta gggttgggct
    14161 ttcggaacgc ccgatccaat gcggtctgcc atgcggaggt agagcaggtc ttgcatatgt
    14221 tctgcgccca ctttgcggat aagacggcgc accgacgact cggttacttc atctacatta
    14281 tagtaaaaca tgtggtaacg aataaggtcg gaaatgagct ctatttgttt gcgcggaaac
    14341 ttgaggcggg ccatcaggtc ccgcgccatg cgagatccca gcgcttcgtg gttataaaat
    14401 gtggagtcgg gtccatcccc ttgctttact cttggttttg ccacatcgtg cagcaacgca
    14461 gccaaacgaa cttccaggct ccagttctgt tctgtcgcat attggagcga gcgaaggttg
    14521 tgttcccaca cggtgtactt atggtgtttg ttttgcgaaa cttcatatcc ttcctcgagc
    14581 tcgggtgcaa taaaggaaag caacttgagt tcccgcaaaa gctcaatgcc ggccatggct
    14641 tgttttgcca taacaatttt gcagaattcg tctctgatgc gctctttgga aatttcctgc
    14701 aggagggcgg cgtttttttg tattgcttct tttgttttgg gttctatgaa aaacccgagc
    14761 tgcgttgcca gtctggcagc gcgcatcatg cgcagagcat cttcctgaaa gcgggcttcg
    14821 ggctctccta cggctcgaat aatttttgct ttcaagtcct cttgtccgcc aaaagggtcg
    14881 ataatttctt ttccccgaag cgcaatggcg tttaccgtaa aatcgcgccg cgaaagatcc
    14941 tcttggagcg tttgggcgta tttcacttct tctggccttc tgcgatcggt atacttgtga
    15001 tctgatcgat acgtggtaat ttcgatttcc ttgagtttag gattttggct ttgtgtaaga
    15061 acagttaccg tgaaaaactt gttttcgtaa aagctttcgg ggaaaatctt ctgtaattct
    15121 tcggggcggg cttgtgtggt aacatcccaa tcttcgggtt ctcggcccaa aagcaaatct
    15181 cgtacgcacc cgcctaccac ataggcttca aacccttgtt tttccagcgt ttccccaatc
    15241 tcttgtactt ctttcgggat tgccttcatg gagtgtatcc tagcttattt tcttgaattt
    15301 ttcaattcaa aatgctagaa taagggtaat ttgagagcgc ccgtagctta acggacagag
    15361 tgctgggctt cggacccagt gatgagggtt cgattccttc cgggcgcacc agaaaggatt
    15421 cgcatttaat aattaactaa taaacatatg agtattgaaa acccattcag caatccaaca
    15481 cctccgcaag aggaagctgc aaaaccagaa aaaagagaaa gcggcgaaga cctgaaagaa
    15541 aacgacctaa taattgcgga tgatcagctc gaacttgcta aagacattct tgaggaaagt
    15601 catatcccgc atgaagacaa ggagcataaa aagagagagg aagtacgcgg cgaggtagac
    15661 tacgacgttc ccggagaatt cgtagtaagc atcaataatc ccgagcgccc cgacgagccc
    15721 gcagattttg aattcacaaa gcaagttttc caacttttca aggattcgga aatagctgta
    15781 cgttatggaa aaaatccaga acgcggatga tgtaaaccat aaattaattt ttgatcttgc
    15841 cgtcaagcta ctccatgctt ccgcaccagc ttgacatgcc aaagagcttg tgatacaata
    15901 gcactattga aatgagaatg gtgtccgctt ggcgggcatt gttttgtaaa caaccatgat
    15961 agactttaaa aatttcgcat tgcttttgga aagtatcgcc gaagaaaaag ggctttccaa
    16021 agaccgcgtt attgaaactg tagagcaggc cattgctgcc gcgtacaaaa aagaattcgg
    16081 caagcgaggc cagaatatca aggcatctct taatatccaa acgggcaata ttgagttttg
    16141 gcaactcaaa gaagtggttg accaagacat gatttattca gaagaggaat tagaggcaat
    16201 gaaaagcggc gaggaagacg cgtcttcgga agctcccgca aaagaggaag aggccgaagg
    16261 agaaaagaaa gtcaggttta acccagagcg ccatattatg ttggacgaag ccaagaaaat
    16321 acaaaaggga gcgaaagttg gcgacgaaat tgaactcgcg ttggaaacaa aaaccgaata
    16381 cggaagaatc gccgcgcaaa cggcaaaaca ggtaattctg cagaaaatcc gcgaagcaga
    16441 gcgagactcg gtgctcgaag aattccagtc gaaagaagaa gagctggttt cgggcattgt
    16501 gcagcgcgta gaaggaagaa tcgtgttcgt ggatttggga aaaacgctcg gcgttctccc
    16561 tgcagaagag caggtgccgg gagaatttta tcgcctgggc cagcgtttaa agttttacct
    16621 tttgcgtgta gaagattctc caaaagggcc catgattttt ctttcgcggg cgtaccccaa
    16681 gtttatctcc aagcttttcg aactcgaagt acctgaaatt gcggcaggaa ccgtggaaat
    16741 taaaggcatt acaagagagg ctggttctcg cagcaagata gcggtagctt cccatgaaga
    16801 cggtgtggat ccggtaggtt ccatggtagg gcaacggggg acaagggttt ccgcggtgat
    16861 caacgaattg ggaggcgaga aaatcgacat tatcgaatgg gccgaagacc ccgagtttta
    16921 cattgccaat gcgctttctc ccgcaaaggt gttggaggtg gaagttcagc ccaaaaaccg
    16981 tgccattgcc attgttgcag aagaccagct ttctttggcc attggccgcg acggacagaa
    17041 cgtacgcctg gctgcaaaac ttaccggctg gaaaatcgat gtgcgcacgc cagaaggaag
    17101 acaaacgggt gcgagtgaag aagcagacga ggaagaaagc gatgccaaag aggaggtgtc
    17161 ctctgaaacc ccggcgaagg aggaataatc tatgaagtac tcgcagaaag atcttggaaa
    17221 atcgcaaatc gagattgacg tggaacttgc aacagaggag ttcgaagcct accggccaaa
    17281 agcagtggca gaactcgcgc aaagccttcg ggtagaaggg tttcgcccgg ggcatgtgcc
    17341 tgcagcaatt gccgcaagag agctcgcgga aaccaacgtg ctcgaagaaa tggccagaca
    17401 ggcgatttct gaaacctatc gcactattgt gcaagaacag aatctgcctg ttattggaga
    17461 tccaacagtg gaaattcgaa agattgctgc aaacaacccg tttgaatttc gcgtgaaggt
    17521 tgcagtggtg cccgaggtgc agctccctga ttacaaggaa tccgcaggcc aggttgcgag
    17581 aaagcaggtg caggtggaag aaaaggaaat agacgaagcg ctcacctggt tgcagcaaag
    17641 cagaaaagcg caagacagct ccactctcga gctttccgat gagtttgcgc agtctctggg
    17701 taactttgct aatctggccg cgttgcggga aggcgtaaaa gaaggtttgc agcaggaaaa
    17761 ggaaatgaaa gagacagagc ggttaaggca ggaaattgtt gagaagatcg ccgaaaagtc
    17821 acaggtcgcc gtgccggaag ttctcgtgga gcgggaaaag gcagtgttgc tcgacaacgt
    17881 gaagcgcggg gttgccgaag tcatgcaaat ggagtttgca gaatacctgc aaaaatccgg
    17941 caaatcagag caagagctgc tcgactcttt cgccaaagag gcggaacagc gggtaaagcg
    18001 atttctcgta ctgcgcgagg tggcaaagaa agaaggtatc gagccaacca aagaagaggt
    18061 tgagcaagag gccaatcaaa ttcttaacca ttatgcttct gcgaaaaaag cgcaaaaaga
    18121 gattgacccc gtcagattaa aggaatatac ggaaggcgtg atacgccacg aaaaaacatt
    18181 acaattttta gaacagtttg cgtcaccaaa cgtatgaact tgatccctac agtaatagaa
    18241 aaaagccaat acggagagcg agcctacgat atttactcca ggcttctgaa agagcgcatc
    18301 attttcctgg gtgcccccat taacgacatg gttgcaaact cggtaatcgc gcaaatgctg
    18361 tttttggctt ccaaagatcc caaaaaagac attcagttgt atattaatac gccaggcggc
    18421 ctggttactt caggccttgc catttacgat accatgcagt ttgtaaaatg ccccatttcc
    18481 acggtgtgcg tgggcatggc agcttccatg ggggcggtgc ttttggctgc aggcgagaaa
    18541 ggcaagcgct ttgccctgcc gaatgcccaa atcatgatgc atcagccctt gggcggagct
    18601 acgggccagg cagtggaaat tgaaattacc gcaaagcaga tcgtaaaaat taaggagcgc
    18661 ttaaacaaaa ttttggcttc acacacaggg cagtcctacg aaaaagtgga gaaagacacc
    18721 gaccgcgact tttacctcac cgcagacgaa gccaccgaat acggccttat cgacaaggtc
    18781 atcagggaaa aataagagtg gtttttgaca ggcggaacga aatctggtat tctctatact
    18841 agagaaatga caagtcaact gataaatcga atatatgaca gagcaattaa tcctgcttgt
    18901 ggcaggcgtg atttcgcttg cgacaggcgt tattgttggg tactatgccc gacagtccat
    18961 tgccaagaga agagcgggga cggtagagca aaagctccag caaaaagtta acgaagcaaa
    19021 ggaacaggcg gatcagatta tagtcgccgc cgaacaaaaa gcaaaagaag tcgccgaggg
    19081 ggcgaaatca gaagaagaac agcgcaggcg cggatttctc aaaacagaac aagtgttttt
    19141 acaaagagaa gaaacgctca ccaagcgtat ttctgacttt gaggaaaaag aagacacatt
    19201 ccgtaaaaaa gtagaaaagc tccagagcgt cgaaaaggac ctcgaaacgg cgcaagaaga
    19261 ggtaagcaag aaactggaaa gcgttgccgg gctctcgaga gaagacgcaa aaaagcagat
    19321 tttcgatcgc gtcgaaaaag aatacgaaca agacattgtg gggcggatac gcaagttgga
    19381 atcggaaggc caggagcgtt ttgagcgcag ggccaaagaa attgtggctt acgccattca
    19441 aaaaaccgcc gtgtctcaaa cccaggaaat taccaccacc accgtggtac tgccttcaga
    19501 agacctgaaa ggcaagatta ttggaaaaga aggaaggaac attcgttcgt tggaaaggct
    19561 ggctggcgtg gaaatcgtgg tagacgaaac gcccgaagcc gtggtgattt cggggttcga
    19621 cccgttgcgc cgccacattg ccaaggtggc tttggagcgt ttaatcaaag acggcagaat
    19681 tcatcctgcc cgcgtggaag aagaagtggc aaaagtgcag gctgaaatcg acgggcaagt
    19741 gaaggaagcc ggagaagctg cggcctacga ccttgggatt gtggggttag accccaaact
    19801 tattcagctg ttgggccgcc ttaagtttcg caccagctac gggcaaaatg tgctgcttca
    19861 ctccattgaa gtggcgtatt tggccagcgc tctggctgcc gaagtagggg ctgatgtaac
    19921 cgttgccaaa aaagccggcc ttttccacga cattggaaaa gccttagacc accaggtaga
    19981 aggctcgcac gtggaaattg gcatgcgggt gctggaaaaa ttcggtgcgg aaaaagaggt
    20041 aatcacggca atgaagtcgc accacgaaga ttacccgtat gaatctatcg agtccatttt
    20101 ggtgcaggca gccgacgcca tttcagcctc gaggccgggc gccagaaaag atacgctcga
    20161 aaactactta aagcgcattg gcgagttgga aagcctggca aacagttttg aaggagtgga
    20221 aaaggtatat gccattcagg cgggaagaga aattcgcgta tttgtgcatc cagaggatat
    20281 cgacgacaca ggcgctcaaa aactcgcccg cgacattgca aaccgcattg aagaagagct
    20341 tcactacccc ggcgaaatta aggtaaccct tgtgcgcgaa aaccgtttcg ttgagtatgc
    20401 gaggtagcgg tcgatagtct gtgcaaaaag cgcctgttgc aaggcgtttt ttgttgtggt
    20461 agaatttttt tcgacatgga agaagcaata caacatggag caggcgttac cacctttctg
    20521 tggattgccg ttatcttgct tgccgcgaaa cttgcaagtt tagtagaaaa attcggccag
    20581 ccttcggtat tgggcgagct ggtaatgggt gttatccttg gaaacctggt tctcttgggc
    20641 atcaatgtgt ttgagcccat aaagcacgac tccattattg cgtttctggc agagcttggc
    20701 gtggtcatat tgctattcca aattggtttg gagtcaaata ttgcaaaaat gcgcaaagtg
    20761 ggcgtgcggg cgtttctggt ggcaaccatt ggcgtagtag ttccctttgt tttgggaacc
    20821 tatgtggtag ggccatggct tttgcccggc ctcgaacaaa acgcctatct tttcttggga
    20881 gcggcgctta ccgcaacctc ggtggggatc acggcccgcg tattccagga cctaggcaag
    20941 ctgcatattt cctcggccca aattgttctt ggagccgccg tcatcgacga cgtcataggc
    21001 ctcatcattt tggccgtggt ttcagcctta gtaacggcag gcgccgtaag ccttggcgtg
    21061 ataagcttga ttgtggcaaa agcaattttc tttttggtgg gcgccattgt ggtggggcat
    21121 tttgcagctc ctttccttgg caaaatgctt tccaaaatta atacgggtgt tggcatgaaa
    21181 tttgcccttg ccttgagctt tggcctggtg tttgcttact tggcgcaagc ccagtttggt
    21241 ttggccccta ttgtaggagc ttttgccgcc ggcctggtac tggatcccgt gcatttccgg
    21301 ttttttaaag aagagcgaag cgtagaggac ttaattgaac cagtggcgtt tttccttgtg
    21361 cctgttttct ttattgttac aggaatgtcg gtagatttaa caacgctgtt taaacctcaa
    21421 attttactgg tggcattggg aattaccgca gtagcctttg tgggaaaaat tgtttcagga
    21481 cttgcagcag gccggggcga ccgcctgctg gtaggttttg gcatgattcc ccggggagaa
    21541 gtgggcttaa tctttgccac cattggagct tcccttggtg tagtttcccc agacgtgttt
    21601 tcggttatag ttatcatggt aattcttacc acgcttctta ctccacccat tttgaccttc
    21661 ttgcttaaac gccagcctgc cgcggctgtc gcatgagtgt ctcaaataaa acagcccgcg
    21721 atgaaagttg cgggcttaag cgaaaaaaaa gtgctaggca gcctcgtgtg gtgcaaggca
    21781 cgaggctgcc cgacgagagg tgcaactccg gagttcaggg cccccggaga gagaaattcg
    21841 tagtcatgcc aggagaattc cgtacaagct ctgtgcgctt aaagcaaaaa cggaatcctc
    21901 ccggcaactt ctttgtagca gagctcggta aaatgtcaat aaaaacagcg ggttcaaaaa
    21961 tgatagcccg ctccttttct cctcggccac catgctctgg ggtgtggtgg gtgaagtctc
    22021 atgatctcga tagtgctgaa tggggggtcg aatccaagat agaaggtccc cgaaattaaa
    22081 gacttttctg ttttctggtc gaaaacattc gtcgcagata cgtttcggtt ttcatctacg
    22141 ccttgcaggg tagcgatgta ggcgtcggct atttctccct cgattcgccg tataatagca
    22201 acagaacttc ctactaatgt ttgcaatcca gccatggcgt tcacctaaag gtacgaatac
    22261 actatagtac gcgcacccaa ggttaatgtc aatgggaaac cactgtttga agttaagctt
    22321 caaacagcac aggctttgga gaattctatt tcctatttgc gaggagtttt gcaacgccga
    22381 gatggaggac aaaaagaggt agtggcaaca gtaaaagaaa tggtgccgcc ttaacgttcc
    22441 caatggtgat tactagctca ggagtcgaat aaacatataa gaaagctaag gctgctagaa
    22501 ggacaggtag gaatgcggca agaataaaga gtgcaattct tctgggatga tatttcgaag
    22561 gggttaatcc ttttgtcttt gaatagataa accgaagtat aaggtaagat gtgacattta
    22621 gtataaaaat ttgtacaaag gtaattccaa cgaaaagtac cgtatcaccg ctctcgttta
    22681 acgcagggat tgcccagttg gccgcaataa aatacgaaac gaaccataaa actgcaagtt
    22741 cgacaataag aataaagaac ggattcgtat atctcatatt cctatagttt atcttactta
    22801 tccttactgt caattgaatc tgtgccaaca aagatgttac cagagaaagt tttaccaaag
    22861 agggtggctt ccgttgattc acaagtgagg gtcgtttcat tttggcggaa atgagacacc
    22921 aaatcaacat cgccatcttt gtccacgtct tcttcatgcc gcgtcattgc cttgttcttc
    22981 ccacttttgt gcgtttcact cgcgccctca aaaagtacag tggtatggtc tacggttgta
    23041 gcgtcgaaag catctgtagt taagatggct acagggatta caccattccc tttagcttcg
    23101 cagttcatag gattggtgtc ggaaccaggc ttaatatcaa tttgtacttc gtgtacagag
    23161 aatatatctc ctgcttgaac tattgtagat ttaccgttga ttacaagagg aatttcccct
    23221 tcaccagagg tttctataac aagttgatca tcaacaacgt catatttcaa aatttgtcca
    23281 gcgcttactg ttcctacaac ctcgagagtt cctaccgtta tttggagctc ggcgcttccc
    23341 tcttctgctg caacttccat actcccacag gtagttgttc cgatgaatgg aacgaatttt
    23401 ggtatagaaa ggaagggccc aataaccggg agagttccta aggtgataca tttaagtgta
    23461 tcgaatttga tggttgcgcg ttcgctgccg aaatccgcag taatatgtgc gcctccaact
    23521 gcagcatctc taattttaat gtcgcgatcc cctctatttg taatggtacc agaggtattt
    23581 ctgccgagtg gtccgggagc atcaaagtca ttggaaggat catttggttg tgtatcgacg
    23641 ttgttccaaa ttccatcaaa gtccccatca cagctatcgt tatagaagtc gtcccaagtt
    23701 attgtgttta agttagtcca agaaataggg gtaggccatc tttctggttg ttgaagtggt
    23761 cctcttggac tttcttttac gaacggaaca tcatcttcgc cccatcttcc accgaacaaa
    23821 agccatttct gtggcgttgt gctgatcgaa ggtcctccaa gaatgctatc gataacgatt
    23881 ctgaggtcaa gggttcctag tttcttggca gaagttccgc gatcgctccc aattcctgtc
    23941 ggaatagtta catcacatac gcctcgttgg aaatgatttg catgagatcc ctctgcaaca
    24001 taaactactg gtctgtcgtt ttctttttct acattgtccc aattacttcg atagccacca
    24061 aggtgggcag agtaagccga tctttcaggc caaatacttt gtttaacgac ctcttcgata
    24121 gatttattct gttcgaatat taactggata aattcccagt ctccttcgtg atcagggcct
    24181 ccttcccaac tattgtagta gtagaaaaac caatattgca ggacaatgtg gccttcatct
    24241 tcaagaatgt gagcataagc agtgttttta taagggatat cagtaaacgg aatgtgttcg
    24301 ataaacgtgt taccgtccag attgagtttg tagtggcttt cggtacgaga gcttaagtcg
    24361 gtaggggcaa gtgggctcgt ctcttcgact atgtgatttt ctgtattaac aatatctgcc
    24421 gtgtccaaga cgacttggat ttcctggggg cggaagcgct cttcttcgtg aaaatacaaa
    24481 attggtttga acttttcagc gaggagttct tcgaagttta cggcaggagg aaaaatgggc
    24541 ggtgcggtag tagcctgaag cgtcgcatcg gcatttcccc aggaaacatt gtcgttttcc
    24601 cggggagaga ttgtgggctg tatgaggagt gactgtacgc caatgccatt gccaaaagat
    24661 gcaaatacgc tttcgacagg gctaatagag gccgcgagaa ccgaagtttc aacagatgcg
    24721 tctgcatctt caaaaacagc aacagcctgg gcgtttgtta ggctcgaagg gaaaattaca
    24781 agactaaccg cagccgagaa taaaacgaaa aacaagcgca ttggacttgc aagttaaggg
    24841 atttgatata ccacggctac tccaaggaaa tgattggcgg caactccccc tacaccattc
    24901 tgaatctcga aggtatatcg tggtcccggg tcgatcgaag gaaggttttg gatgttaaat
    24961 cgattattcc caagaggagg ttcgagttgt gtttgagtgc catcgattga atgaagagtg
    25021 agagtcccac ttgtactgct agaacccgat tgtttattgc cgttccagga gtaagaggga
    25081 ttttcggaaa atacggctcc tgcaggaatc gtcatgtaca tgttacccac acctcttttt
    25141 gctgccgccg aaaaatgcgt ggccgaagga tagttccagt gataccacga ggcgtcggta
    25201 ggggcaggag cgccaacggt agtagttacc gctacaagcg catcactaag cacagtatct
    25261 agagggttaa ttataggatc caaaagatgc cagcgaaaca tatcggcagc aagtttatct
    25321 ttggtaaggt aggctatgat aaagccgtct ttatccacgt acacgcgaac gtggattgat
    25381 attggcgcag gcgtaccccc tatttcggtg cgggaaatgt tgaaggcgcc gataatgtag
    25441 ttgtcgccgt tttggattgt ttcgtcgaag atagggctaa ttgtggcaaa atcaataagg
    25501 ctggggtcgc gctgcacata agagcttagg ccagcttgag agtcggggag ccctgggctc
    25561 ccgctttgtg ccaggcttgg gcgaataaga ggaatgccaa agagcgaggg tatttcaccc
    25621 tctttttgaa aggcagcgaa gctatagaaa gcaaaaacaa ctgcgaccaa aaatagcaaa
    25681 actgcgcccg cgaggggaag agaaattttg tgtatcatat atgcttatgg taacaattgg
    25741 ggcagaggcg tcaaccccgt tagagacaga ggtactgcga gccctcgcgg tctctaacgg
    25801 ggtcaacctc tcgctacgag tgttatgtgg ataacataca ccagtttatt gtcatggcag
    25861 gaaattggtg aattacttag acgacttcgt atgtctatag gggaaaaaga gagttgcggg
    25921 agagggcgag gtgtgagttt gtacctactt agaacttcgt cccatcttca gcccaccagg
    25981 gtaagcacca ccactgcaaa gcagtggttt tcttttttgg gtacaatttg catgcaaata
    26041 gaaaaccacg cagattgggc gtggcttggg ctttgtagta aggtgctggg ggtctagccg
    26101 gctagggctt gagcgagttg gtggatgcga taaggaggca gttttacaaa gggccgtttt
    26161 gccaaaactg gcacaatcac acagtcggcg tatggtagga cattctccgg cgtgtgggta
    26221 atcacgccga gtgggcgtcc atcgagtacc cgtttcatga gcccgaattc gttgatgcct
    26281 acaacaattc tatccttctc tttggttagg acaacatcaa catgccgggc agcgaaatat
    26341 gcagcaacac caggaagcca cacgggttcc tggagctcgc caaaaagcaa agtatctttt
    26401 gccccatatt gtttttgcca ggcgcgcaat tgcttgattt gcgcatctgg ctgctctgct
    26461 atcacattga tgttgcagtt tagccagacg ccggacacgg cgtctgctgc cgcctgttgg
    26521 atagcctgtt cgttgtcgag tttaggatac ttgattccta cccaccagtt agggcaagcc
    26581 ctttgtactg ctaggtaaca gccctgtagg tcctctacag aaacatcgtt atggccaacg
    26641 ataaataccc cacttactcc tacgtcgcgt gcaatgtgcg cgttatgtac gattaggttt
    26701 tcgttgcctg caagcatggt aacgagcagt ggtttttggg ggaaaacatt cctgaaagca
    26761 atcatactgg tcctcctgtc atacggtgtt gttgtcagta tgcctaaaag ctttgcaaaa
    26821 gtcaaaacaa aaacgggaca tataattatg cctcgttttg gtttgctgga gcgggtgatg
    26881 ggaatcgaac ccacgtagtc tggttggaaa ccaggagcac taccattgtg ctacacccgc
    26941 aaatgctgag ttgtgtcggg gtacccagat tcgaactggg actacatcct cccaaaggac
    27001 gcgtgctacc gttgcactat accccgtgct gtggattata ctctagcatt tttgcggagt
    27061 tttttcaatc tgtggtataa tttctcaatg acgtttggag agtttttacg gaagttaagc
    27121 atagcggaag agtgcaggag atacaacact tctttgtggc aatgtcccca gtttcttttt
    27181 actgttatgg ggctgcttat tattctttcg gtagtattct ctttcattct gggaaaccgg
    27241 tatattcggg atccggaaat taccctttta tttattcttt cgttaacggg cgtgctgttt
    27301 gctatggcgt ttattatcac ccgcagcttt gaacagctgg ccgaagccaa ccgcctgaaa
    27361 accgaattta tcggcattgt gtcccatcag ttgcgttctc ctcttacgaa cttaaagtgg
    27421 ggtttggact ttttaatgac cgaaaccacc cagcacaccc agcaggaaga gggatatttt
    27481 cacattttaa aagataacgt gaacaggatg catgaaatgg tgaacgacct gcttaccgta
    27541 tcgagggccg aacagggcac gctccccttc caggaaaagg aatttgactt tgcagagctg
    27601 gtggaacaga tgctggctga attccagtct gcaattcagg cctcgaacat ccaggtgaaa
    27661 gtagaaggag agcgcgtccc aaagctttac aacgatcctt ctttggtgcg gcacattctg
    27721 ggcaacctgg tggacaacgc catcagatat gcctggcgag accacgagcc gatcccaaac
    27781 ggagacagga aggcgcaaat tgttattgcg tatcgcggcg cgggcgaccg cttgcgcgtg
    27841 gaggtacggg acaacggcgt gggaattccg cggcaagatc aaaagtacat tttccaaaag
    27901 tttttccgtt cgacaaacgc agtaaggcgg caaacagaag ggtcggggct cggattgttc
    27961 attgtaaagt cgcttttgga aaaggccgga ggcgaaattg gatttgcatc agaggagaac
    28021 aaagggtcga cgttctggtt caccctccct tttaaaaagt aactaacaaa cacatgaaac
    28081 gcatattgtt tatcgaagac gaagcagcca ttcacaaaac ctttaccgac gtgctcgcgc
    28141 gcgaagacta tgagattata tcggctttag acggggaaat cggactgcgg ctggcaaaag
    28201 aaaagaaacc cgatcttatc ctgctagact taatactgcc caagatggat ggttttgaag
    28261 tactcaaggc gttaaaagca gacgaggaaa cgaaaagaat ccctgttatt gtgctcacca
    28321 atttggagca aatggaagac atccagaaag cgattgatct gggagcgcgc acctacctgg
    28381 tgaagtcgaa ctacgactta aaagaggttg tagatatgat gaaaaaagca atcgaggagt
    28441 agggaacacc caattgttca tgaatgttga gccgggacaa ctaaaagctt ttctcgtgga
    28501 cgcggggctg gtaaaagaaa aagatttcga ggaagcggta aagaaatcaa agaaaacaac
    28561 aaaacctttg gcagacgtgc tgctggcaga gggttttttg tcggaagagg agttgatgaa
    28621 aaccgaaggg tatattcttg gcattccttt tgtaagcctt acggatgaaa agattgatcc
    28681 tgaagtactg aagattattc cggaagccat agcaaaatcc cacaatattg tggcttttcg
    28741 caaaaaggat tcagaactgg aagttgccat gctagatccc gacgacctgg gaaccatcga
    28801 ctttattaaa aagaagtcga atttgaaaat tctcccgcgc cttactacgc cagagagcat
    28861 aaagtatgta ttgcgccagt acacgaaaac ccttcaggcg gagtttggcg atattattca
    28921 aaaagaggta accggcatta aagcgctcaa agagcaagga ggaggccagg aagccgaagg
    28981 caaggagtta gagcgggttg cagaggagct gccggttatc cgcatagtgg acacgctttt
    29041 aaaacatgcc atattgcagc gggcctccga cattcatatt gagcctaccg aaaaagaagt
    29101 ggtggtgcgc taccggatcg acggcgtgtt gcgcgacgcc atggtgctac ccaggcaggc
    29161 cgcagccgga attgtcgctc gcattaaagt gctttcgaac ttaaagttag acgagcaccg
    29221 tttgccccag gacggccgtt ttaaaataga aaccgatgag tataaatatt cagcgcgtgt
    29281 ttcggtgctg cccgtgttcg acggagaaaa agtggtaatg cgtttgcttg ccgagggatc
    29341 taaagccttt tccttgggag agcttggctt tcgcggtgaa gccttagagc tggtgcagcg
    29401 aaaccttaag aggccggtgg gcatgctttt ggttaccggg cctacgggat ccggaaaaac
    29461 caccacgttg tatacttcca tggaaatttt aaatacgccg gaggtaaata tttccaccgt
    29521 agaagatcct attgaatatc gcatgccgcg cgtgaaccaa acccagataa atcccaaggt
    29581 gggcctgacc tttgcttccg gtttacggtc tttgttgcgc caggaccccg acgtgttaat
    29641 ggtaggggaa attcgcgacc aggaaaccgc aaacctggcc attaacgcgg cgctcaccgg
    29701 gcatttggtg ctttctacgc tccatactac gaacgcggca ggaagcattc cccgtttgat
    29761 cgatatgggg gttgaaccct ttttaatttc ttccacactg aaccttgtgg ttgcccagcg
    29821 tttggtgcga aagctctgcg aagagaaaga agcatatcag ctttcagaaa aggatctcga
    29881 ggaacttgcc aggtattgca atttggaacg ggttctcgaa gccctggttg cagagaaaat
    29941 catcaaaccc aaacaaacgc tccgagacat cgattttttc aagccaaaac ccacggcaga
    30001 cttccccgac ggctattcgg ggcgcctcgg gatttacgaa gcgttgccgg ttaccgaatc
    30061 tattaaggaa ctcattgtgc aaaatgccac gtcagacaaa atcgcccacc aggcgcagtc
    30121 ggaaggcatg ttgaccatgg tggaagacgg ttttgtaaaa gcggcgcaag gcctcacaac
    30181 cttagaagag gtgttgcgcg ttattatgga ataattattc tatgccccaa tttacctaca
    30241 cagcaattac cgcagaggga caaaaagtta cgggtacgga aacagcagaa tcagagcgag
    30301 acctgggaag aatcctttac gaaaaagggc atattctcac gaccgtaaaa gcaggggaaa
    30361 aagggaaact cgaactcccc acactaaaag aggcgttttc gtttttgcaa cgggtttcac
    30421 tcatggagaa actgatgttt acccgaaatc ttcaggtaat ggtggcggcc gggattcctt
    30481 tgcccaaagg cctcgacgtg ttggccgaac aaacagaaag ttcaaaattt cggcgggtta
    30541 ttattgatgt gaaaaaacgg gtggtggaag ggcaaacact ctctttatct atggaagcct
    30601 acccccatgt gttctccgaa ctcttcacca acatggtaaa agtgggcgaa gagtcgggaa
    30661 ctttagagaa tgtgttatcc cagctcacgg ttcagttaga gcgccaacac gagttgaagt
    30721 caaaaattat gggagctttg ttgtatcctt ccgttgtgat aaccgccatg ataggcattg
    30781 gggtattaat gttaatagcg gttattccaa aactggcaga aacattcgaa gaacttggcg
    30841 cagaactccc ccttaccacc agagtagtaa ttgggctaag ccgttttctt gcttcgtatt
    30901 ggtttatcgt atttccgata gtgctcgttt tgctggtgct gctctttcgt tttattcaaa
    30961 ctggcacggg caagcgcctg tttggccaga tgcttttgca tgctcccgta ttctctggca
    31021 ttgtacgaaa gttcaacgcg gcgttaatga cccgcaccat aagttcgctt attgaagctg
    31081 gcgtgccttt ggtgcggggg ctcgaaatta cctcgaaagt ggtggggaac agcttctttg
    31141 aagattccct gaaagaggcg gctgtcaggg tggggaaggg atcgaggctg tctgaagtgt
    31201 tacgggagta ccgtagtctg tatcctgtta ttgtcattca aatggtggaa gtgggagaag
    31261 aaaccggaga gacaggaagt atcttggcaa aacttgcgga gttttacgaa gaagaagtca
    31321 cgcaagtaac caaaaacttg acttcgatta tcgaacccat attaatgctt atcattgggg
    31381 ccgcagtagg ttttttcgcg gtttccatga ttcagcccat gtattcgatg ctcggagcca
    31441 tacagtaaca atatgagagg ctttacgcta gctgaaattt tagtggtggt ggctattctt
    31501 ggactcttgg tagcaatgtc gttgccggta ttcagcattt tccgcgaaga aagccgcgtg
    31561 acaaccactg cagaagaggt tgcgagtatt ttgcgcctgg cgcagaacag aacactggct
    31621 tcccagggcg acacgaagca cggagtgtac tttgacaata tcgccagccc ccaccagtac
    31681 gtacttttcc aaacaaacac cgactacgcc agccgtaatg ccccagaaga cgaagtgtac
    31741 gagatttcag acaaactcga gttttctgtt attgattttg caggagggca ggaagtggtg
    31801 ttcgagcgtt tgaccggagc cacaaaccag gctggcagcg tgacggttcg ggtagcttct
    31861 gatcctgcga aagtgagaac cgtgtttgtg gaaagttcgg gcaacgtgga aatcggcagc
    31921 agtatttctc ctggagatga cggcaactgt acctccgata cgcccccggg cggccgtgtc
    31981 tgcgataccc gccatgtgca tattgagtat ggcccccgcg ttatcgatac cctaacggaa
    32041 actattacgc ttaggttttc ttcgccgccc gatccggata ttacccacga cattgtaatt
    32101 gctagttcct tgagcggcgg ccaaattgta tgggaagacg atattgacgt gtacctagaa
    32161 aagcagcatg taaagatcca cacgcacaag ctgaatgact ttggcgttac agaattttcc
    32221 attcaccgcg caagcgaccc tattcatacc aagccgttgc ttattaccat tagcggagac
    32281 ttgcccgcgc ccaacaagct cattgagtac gacgcggcgg gcgacattgt tatttcagga
    32341 acagaatcaa ccagtgttat tcttccctat gacatccaat aacaaaggag gatcggttgt
    32401 ggaactgata gttgtggttg cgattattgg gggctctttg gcagccattt tgggattggt
    32461 cgcgctttct ttggtaagct ctggcattgc aaggcaaacg caagaggcag tgtttctcgc
    32521 gcaagaagcc atggaagcgc tgcggaatta tcgcgacgga gtgccgtggg atcaagacga
    32581 ccccttaaac caatatgatg gttttgaata cgtaaacttt gccctgaatc cgttccacct
    32641 tgaaaaatca accgactccc caccccagtg gaagcttgtt tcgggggcgg aaaccgtagg
    32701 gatttttacc agggaaatta ttatggaatt tgcgcatcgc gacgccaacg acgatatcgt
    32761 tccaagcggt acgcaggacc ccgacaccct tgcaattttg gtgcgcgttt catggcagga
    32821 acgcggcaga acacatacgg ttgagattgc ttcttatctt accaactgga aataattatg
    32881 agaaatacaa aagggtttac tatactggag gtggtggtat atacgggcat tttgatgatt
    32941 gtggtaacgg cgctgatagg ctttttactt tggatggtgc aatcaaatac gaagaccgcc
    33001 gtattgcacc aggtggcgga agccgccaga aacgccatag acgaaatgga aaaagaaatc
    33061 agggaggcgg aaagcttgta caccccaacg accagcctaa cccagctttc tctggaaacc
    33121 aaaaagaatg tgcctttggg cgaaacttcc acgtacgttg atttctttct ttgcggcacc
    33181 aggctgtgcg taaaacgcga aggccaaaac gccatagcgc ttacgtcgga aagtctggat
    33241 gttaccaatt tgcagttcca ggaagtacga acgggaggcg tgtcttcctt gagcattatt
    33301 ttcgacgttg agcgccagaa tccggggaac catcccgagt ttgcggcttc catacaagca
    33361 agagcagttg tttcgttacg ggcttatgaa taaaggattt gtagggttat acattacgat
    33421 tattgtgtta atggtaacgc ttgccgtttt ggtaagcatg ggatttagtg tggttgtaca
    33481 acagcgcatt gcgcaaaata ccgtgcagtc tgcccaggcg tattacggcg cggaagccgg
    33541 tgtcgaagac gctctgatta gattgaaatc agggaaaaac gtatgcaacc caccaggatc
    33601 tcccccttgt tccaactcca tttccttgtg gagcgggcca gcaatcgtaa taaccgtttc
    33661 cgacgttgta ggaggcgcga gaacggttac cgcagaagga gatgctacaa accgtttccg
    33721 caccgtgcaa acggtgtttg agatttcttc tacaggggta ggttttaact atggagcgca
    33781 gataggcgag ggcggccttc aaatggataa taacgctatt gtgaacggca atgtgttttc
    33841 caatggaaat gttatagggg aaaacggaag ccgaattacc ggcaccgtac agatagctgg
    33901 accagggaaa atactggata atgtagatat cggaaccgtt ggcattggag gtgatgcata
    33961 tgtggatatt tgtcaaaata gcggtagcgg cacgagaatt gagggagatc ttcatacaaa
    34021 tagcgctggc ccatgtgtgg tagtcggctc gacgacaagt tcgggactac caattgcccc
    34081 aattcctttg cctattctcg atagccaaat tgatgcatgg aagttagatg cagatgatgg
    34141 ttttcccttg gtaaacatta cggttaccga tacgttacca ggagttgacc tttcgcttgg
    34201 ccccaaaaaa ataacagggg agctgatcgt ggacaacaat gccaccctcg aggttacagg
    34261 caccctttgg gtcgttggca aagtaaccat aaaaaatggc gccgtcgtgc aacttaatcc
    34321 tggctatggt acttcaagcg gcatgattat ttcaaatgat attgttttgc tggacaacaa
    34381 cagcgtctcg aaaggctcgg gccaggctgg gagttattta atgtatattt ccacttctac
    34441 ctcgagtcag gcaattgagt ccaaaaacaa tgcgaaagca gacattctgt atacgaacac
    34501 aggtaccatt aaagtagaca ataacgcagg tcttcgggag gtcgtgggat atcgtatgca
    34561 cctattgaat aatgcggtga ttacttatga gagcggattg gcaaactcgg cgtttacgtc
    34621 ggggccgggg ggcggctggg tggtaacaag ttggaaagaa attgaataac aagatacaat
    34681 aagtactaat ggcaggactt cttacgcttc atcccaaagc atttggttta gatctgtctg
    34741 atttgtcgct caagattgtg agtcttgagg agcgcggcaa agggtttagg ctttcttcgt
    34801 ttggcgaggc aagcattccc gaaggagtta tcgagggggg agaaattaga aaagaagaga
    34861 aacttgtcca ggtcattcga aaagcggttg ccgaggtaaa agggaagaaa atccaaacaa
    34921 actacatggt agcttccctt cccgaagaaa aagggtttgt tcaggtggtg caatttcccc
    34981 ctatgggcaa agaagaactt ggtaacgcta tacgcctgca ggcagaaaac tacataccct
    35041 atccggtgga cactgtgtac ctcgatttta cggttattcc cccttcgcac aatcatgtcg
    35101 accatttaga cgtgcttatt gcttctcttc cgcgcaccat agttgattct tacatttcgg
    35161 catttgagaa ggcggggttt actccccggg cgcttgagat agacgccctt gccttgagca
    35221 gggcagtcat agaaaagggg gttgcgcccg tgcctgtatt gcttgtggac gtgggaaacg
    35281 caagaacgaa gttcagcatt ttttcagggt acagtttgcg tttcaccact tccgtcccgg
    35341 tttctgccaa tacgtttacc caggccgtgg caaccaggct gggagtagaa gaagaacgtg
    35401 cagaagagct caaaagaatg tacggtttgg attccattga agaaaaactg ggcgacgaag
    35461 tttttcaggc catggcgccc tcggccacag agtttgtaaa acaaattcaa aaatacttaa
    35521 cgtactacga aacccatatt ccccaccagc atttagaccg cccccaaaag aagattaaaa
    35581 aagtcgtgct ctgcggaggc ggagccaacc tgcgggggct gcccgaattt cttatgcgtt
    35641 tcttaaaagt ggaagtcgcg gtgggaaacc cctgggtgaa tattctttcc gcttctttaa
    35701 aagagttgcc ggctctcccg ttccaggatt ccctgcgcta tagcacggct ttgggcctgg
    35761 gcctgcgggg cgtgaagaga gaagtttccg atgattaacc ttcttcctcc agaatacaga
    35821 cagcggctca aagacgagga atattttcgc ctcgttctta tcttgggtgc gctccttttg
    35881 ctgtttttcg tatcgttgtc tttgatgctc ctgtcgattc gcatttatct ttcgggcgag
    35941 atcgacgccc aaaaaatttt ggtagaacgc cagcaggaag aatacaatcg cgagaatccc
    36001 ttggaactga acgtaaaaga tcttaatcgg agcgtagccc aactggcggc attttacaaa
    36061 gagcaaattc tgctttctga aaccctacag gaagttacgg ccgctctgcc ccctcaagcc
    36121 tatcttactt cttttgtgta taacgccggc gctcccggca aaatttcatt ggcaggtttt
    36181 gccccgacaa gcgaagactt gcaggcgttc agagggacct tggagcagaa tccccgattt
    36241 tcgaactttt tctttccgct ttcaaactcg acaagcgagc gcgatattac gttttccttt
    36301 acgtttacgg tgcaatgaaa atgaatacca agcgaaattt attgttggca gcagcgcttt
    36361 tcctgggagc ggctttcgtg ttcgtttcct ttgtggtgta tccggtattc cggggagtag
    36421 tgcacgacca cgaaagggcg ttagagcaaa ggagggagtt cgtccagctt ggagaagata
    36481 gagaacacct cgcagaattc gagaggctgt ttatgcgaga gcaaagcgca ttcgaccagt
    36541 tagaacgagt cttccttgat ttgcgctcgc ctattgagtt tttccgtttt ctcgaccgga
    36601 gcgccgaaga gtttggcata accctcgaaa aaacgccggg gctcccccaa accacaccag
    36661 aagaaccctg gacatacgtg gatgtgcatc tcgagggaga aggaacatat cctggcgccc
    36721 ttgcgtttat agaaaagatc gaaagttccc catacttgct cgagctaaaa ggccttaact
    36781 tttcaaaggc agaaggccag ttgttggtgc gattttccat taatacaaga atatatgcga
    36841 aagattagtt ttcccaaacg ttttaacagc aagaaaattt tagcttttct tcggtggatg
    36901 ggggagaacg cttttcttgg gtttttagta ctgcttgtta tcgccttgtg tatatcttct
    36961 ttggtgtttt accgttatgt gttcctttta gagagcgccc agttggagtt ccggccctca
    37021 caaaatacct ttcaaaccaa accttttgag gatattgtgc gagtgtggca agaaaggggg
    37081 caggcttttg agcaggcaag tcctgaaaac taccgggatg cgtttgcacc tgctcccttg
    37141 cctccagagg cggaagaatc agacaattga ccggaaggat ttttttctct ataatatcta
    37201 tgctttgtcc ctatagctca atggatcgag cacgggcctt ctaagcctgg gatcgaggtt
    37261 cgactcctcg tagggacaca agagagaata catggtgggc gtagcttagt ggcttaaagc
    37321 gtcgctctgt ggcagcgaag atcgccggtt caaatccggt cgtccaccca caaaaccaaa
    37381 atacggcagc tgtggataaa tacattatgc cggaagggaa atacgttgca taataaatta
    37441 agatgttgtc tctcctcggt attcttattt ttgttttcat tgccgtgtgg ctccataggc
    37501 gtattagtag gatcgaagaa ttgttggaat caaaaagcgc gcccttactt tcacctgctc
    37561 cgcaacaggc tttgtctcct ttggtgccaa cacagcaaac gcaaacgcct atctccggga
    37621 atgaagaact tgccgtaatc gataaattcg ttgtttgggc aaaagaagac tggttgttaa
    37681 agctcggggc ttttctgttg cttattggtt ttggctggct tgcaacgtat gcatttgcga
    37741 ataactggat tggccctatg gggcgcatta cccttggctt ggttgcaggc gtccttatct
    37801 tgcttctggg atggtggcgg attaaaacct atgtatatca ggggagtaca ttcctcgtgt
    37861 taggctcctc ggtcattttg cttaccacgt tcgccgcaag gatagtctac gacttcttta
    37921 ctcctttagc ggcactcggc ataatgttta tgagcgccgc ttttgttgct tttgcaagcg
    37981 ttatctaccg ccaccttccc cttgctttgg caagcttact atccgcggga gtcgcccctc
    38041 ttcttacgag tcctttgagc tttgcagttt ctttaagtac ggacttcgta actctgttct
    38101 cgtatctgct tgtcgtaaca attggcacgg tatgggttgt cgctctcacc ggatggcgca
    38161 ttcttacgac agtcgcactt atcatagtgg ctttgtatag tctcccccat ctctttattg
    38221 gcagatcttt aggcgttgaa atagttctcg cttttacatt tgcctttacc gctgtgttct
    38281 ttataaccag catactcggc attttgaaac tggcaaagga gaagattgtt cccgacctca
    38341 tcacagcagg cggcaccggg ttattccttc tcgtgtggat tctcatggca gctcccgaag
    38401 agtggaaaag cattctcatg gtggcctgga ttttggtatt cgcctttggc ggatttctcg
    38461 cttttcgcct cacgcaacgc attgaacctt tctacgtata tttgggggta agcattgcca
    38521 tgcttgctgg cgcaaccgcg gttgagttgc aaggagctgc tcttgttatt gcctacagca
    38581 tagagagtgg ggttattgca cttgttgctt atgcgatttt gagggattta cggatcgctc
    38641 aaatgttgag tctgctgtta cttggcccgg cctatctgtc tgtgggaagc gtaatttccc
    38701 gatcgtggaa tatcggtgta gtccataaag acttctttgt actgttcgta ctggcggtaa
    38761 tattcttcgg attggggctt ttcttcttat gggcgcaaaa acaaaaaata agcaacgaaa
    38821 aaaaagaatc cgcgtacttt acggccggac tccttattgt tggctctgca tatatgcatt
    38881 tgcttttatg gctctctctc catgcgacaa aattaagcga tgacgttgcc acgatgtttt
    38941 ctttggtcgc ctacaccgtt atagggctag ccacgtattt ctacggaaaa acaaaagaaa
    39001 agagaataat gcgtttgtac ggtgccgtac tgctaaccct tgtggttggc cgccttctgg
    39061 ttatcgacgt ttggagcatg gctttagcgg ggcgcgttgt cacatttttc cttgttggca
    39121 tactcttaac aaccactgcg tttgtcggcc gaagcaagaa agcattgccc atgcttgttc
    39181 tagctacagc aggagccatg acgttctatg cgagcagtgc cgaggcttta tcgaccgcct
    39241 cacaggaaac gataagcgca tatcgacaac tcaaagagtt gcagcctgta gcgattttag
    39301 tgccaacggt ggtagaaata ccgttcgaaa gccaagagct ggaacgtttt agttttgcgg
    39361 tgttagacga agctaccgat acttttcagc ctgtcttttt taagcaggaa acatcggtaa
    39421 atgaaatacc tataacaacc attaatgcga caaaccagtc ggctctcaat gcgggaagaa
    39481 tggttgacgg aaatgtgcaa acctatactg cttttgctct gcccgaagaa gcgcagggaa
    39541 gcactcaact cattctgcag agtaccaggc ccattacatc ttcctcgctc actcttttac
    39601 tcgacaacca cgttgccttg cctacttcaa tagaaatacg cgcgcaggta agcgggacaa
    39661 ataccatcgt tcttgcaaaa ataccgatga ggcaggccac tatccagttc cccagaacaa
    39721 cctctgcgac atggacaatc gcgctcacct atgcacaacc tcttcgcata tctgaactta
    39781 aacttgcgca agaaaatgct acaagatcca gttcgcacgc actgcgattt cttgcacaac
    39841 ctgggcgtgc gtatcaagta tactttaacc cggaccgata tgtgtcgccg cctgtggggg
    39901 aagcaggcaa cctggcagat aaccagggcg tgcttcgttt gccttcacct ctttcgcaaa
    39961 cgaatgtgta ttataaacaa gcggatgtcg atctcgacaa tataccagat attcttgata
    40021 actgcgtatc ggtgaggaat acagaccagg ccgatgttaa tagaaatgga aggggtgacg
    40081 cctgcgacga cttcgacagg gacggaattg taaatagcca agacaactgt ccaaatgacc
    40141 ctaatcgagg gcaagccgat gtagatgcag acggcattgg agatgtttgc gatactgaag
    40201 aaagcagatt taccgaacgc tacaaatgga ttccgtggct gggaattgcc tttgcagctt
    40261 tgacgcttat tgtacttttt gcgctaaccg cccgatcgac actccaaaaa cgaaatcagg
    40321 acaaaccgcc cacggtatag tctcaagcta caaacaataa acaacaacac ctgccttgag
    40381 ggcagggttg ttttgtttag aaaacggagt ttctcaatcc tattcgtctt cctcctcttc
    40441 tacatccgtg tcgtcgtctg tatcttcttc tgtgttatcg tccatcatac cgtcgtcgtc
    40501 agtcatcccg tcgtcgtcga gaatggagac caatggtgag agaatatcaa aaatcatagt
    40561 ttgaccttta atgttttgtt acaaaactca ttatagaaag taaagaggtc ttgtcaactc
    40621 cccgttttcg cagttattca caaggttgcg gaaaggcacg ttttatggta gaatttctca
    40681 taatgtttgc cttgcgagcc cccgtagttc aacggacaga acaggaaact cctaagttct
    40741 cgatagaggt tcaattcctc tcgggggcac actttgcagg ctcagtgtag acaaacaggg
    40801 gcctatagta aatcggcatt acgcctccat ggcatggagg agggcggggt tcaattcccc
    40861 gtaggtccac aaaggagatg tgccggagcg gttgaacggg gcggtctcga aaaccgctat
    40921 accgaaaggt atcggaggtt cgaatcctcc catctccgca aagcctatgg ctttattcaa
    40981 aaaacaacaa ccagaagagc aaacgccgca ggctttgcgc aagaaagttc aggcgcttga
    41041 gaaaaggctg gaacaaacag cgaaagaact ggaacagtta aagcagcaaa cgcgccaggc
    41101 agttaccagg gtgggtattg tacggtttaa tccctttgcg gagatggggg gcgatcaaag
    41161 tttcgccgtt gcattgctca acgaagaaca cacagggttt gtgctcacca gttactatgg
    41221 aaaagactta aacagggtat ttgcaaagcc ggtgcaaaaa ggcagttcgc gttatgaact
    41281 ttcagatgaa gaaagagagg cgcttgagaa agccatgggt gttgaaaagc caaagagcaa
    41341 gaggagaaag aaaaaatagt attatgatta cacaaaacgc cgaaaatcgt atagccaaag
    41401 agcatacagg agcaagagcg ccggttgtgg tggtgttggg gcatgtggat catggaaagt
    41461 cttctttgct tgaggcgatc cgcgaggatt ttcgcattac tgccaaggag tcgggcggca
    41521 ttacccagca catgggggct taccaggcgc aatatcaggg caagaacatt acgtttatag
    41581 acacgccggg ccacgaactc ttttctgcaa tgcgttcccg gggggcaaaa gtagccgata
    41641 ttgccgtatt ggtggtggca gcagacgaaa gcgtgaagcc tcaaaccgtg gaagcaatcg
    41701 agcaggtcaa gaaggccggc ataccaatgg tggtggctat taataagatt gacaagcttc
    41761 aagccaactc ccaacgagta aaacaggagt tggcgcagca cgaagtaata gtggagtctt
    41821 atggcggaaa tgttccttcg gtggaaactt ctgccacgac caagcaggga atttcagagt
    41881 tgctcgaaat gcttttgttg gtggcagatc tcgaaagcct gaaggcaaac cccgaacaac
    41941 cggcagaggg cgtagtaatt gagtcgtctc ttgaccccaa aagagggcct ctggcaagcc
    42001 ttttggtact caacggcact ttgcgccttg gcgattggct ctcaactcct tcctcgtcag
    42061 ggaaagcgcg catgatagag gattttcagc gccagcagat tgcagaggcg ggccctgcaa
    42121 cgccagccgt agtattgggg tttttaatcc ccccgcgcgt aggagatgta tttcgggcgt
    42181 tcgaaagcga aaccgcagta caagagtttg tgtgggagaa tgagcttaag gaagtggcaa
    42241 agggcgccag cgaggtgaat ccggaagcaa aactttacaa cattatactg aaagcagatg
    42301 tagcaggctc tctcgaggcc ttggaagatg tgttgcacaa tcttccccag ggcgaggttc
    42361 agcttcgcct cgtggaggca ggcgtgggag aaattaccga gaacgacgtg aaactggcgc
    42421 gttcgactac tgctgtagtc tttggatttc gtaccaaagt aagcgtccag gcggctgatc
    42481 ttgcagaaaa ggagcaaatc tctattgaaa cgttccagat aatctatgag cttatccagc
    42541 gcgtgagaga agctatggag cagtcgctga aagacgaggg aggccgccag gagttgggca
    42601 cgctcaacgt actggcgata ttcctggtag ataagactag gcaggttatt ggcggcaagg
    42661 tggttgaagg agaagtaagg aaaggcgtgc gggccgaaat tatccgcgca ggcgaggtgg
    42721 taggccaggg caaggtaact aatgtgcagc acgacaaaaa agacgtgcct tctgtggcca
    42781 aaggaaaaga atgcgggctg tcttttgaag cagcagagaa agtgcaagca ggagatcagc
    42841 tgcaattttt tgtcgtaaga gctcccaaaa agtagtatga agaatcgcat tgcgcaggta
    42901 gaagaactca tgcgccatga gctggcaaac atcattttgc gggaggttga aatgccagaa
    42961 ggcgcggtgg ttaccttaac tcgcgtggtg gctgcgggca acttgcagca agctcgcgta
    43021 tatatgagtg tggtaccaga tgagaagggc ggggaagcgc taaaagtcct ttcgcaaaat
    43081 atctacgaaa ttcagcagat actcaatggg cgactcaaaa tgaggccggt gcccaaaatt
    43141 cagtgggtgc ttgaaacagc aaccgccgag gcccagcaca tcgaagaact cttagataaa
    43201 attaaaaaac aggggtaaaa tatagatatg gcgcgatagg caagtggtaa gccaagggtc
    43261 tgcaaaaccc ttatgcgtgg gttcgattcc cactcgcgcc tccaatgccc gggtggcgga
    43321 attggtttac gcagaggact taaaatcctt gaccgaaagg tttgtgggtt cgagccccac
    43381 cccgggcaca gaaaacaaaa cagcttccga aacggagctg ttttgtttct ttacccttcc
    43441 aaaacgacga ttgaggcgcg cttgatgcca aagtttttgg cgtcccaata ctcggcaaac
    43501 cagatatcta catggttttg caatctggta ttcatgcggt cttccaccac aaatactttg
    43561 ttgccgtaga gctcgggaat tttaatcttc gttccc
//
LOCUS       DASRUP010000077        10354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2290905, whole genome shotgun sequence.
ACCESSION   DASRUP010000077 DASRUP010000000
VERSION     DASRUP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290905"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1459)
                     /locus_tag="VFE94_03165"
     CDS             complement(137..1459)
                     /locus_tag="VFE94_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50118.1"
                     /translation="MRILLISSVVFVALAGAYAVFAQEEPAVEFPVAELGNCQSKEEC
                     HAYCDEVEHIEQCVNFAERHGLMKGGELEEAKKVVSALRQGAQLPGGCRNKEECEVYC
                     KDGAHIEECIAFAEAAGFMSPEELEDAKRVAPLMARGEMPGGCRSREECEAYCSQDGH
                     IEECAAFFEKAGFISVQEAEMFRKTGGRGPGDCRGREECEAFCNAPANQKVCFEFAKE
                     HDLIPQEELQQMQEGIEMFKRGLQSAPPEVAQCLKETVGEDVLAKIGAGTFLPSRELG
                     EHMRQCFETHLPNIIQQNIQEQVGGEVPPEAADCVQRILGDTRELQGPPTAEQEQRIR
                     EECFPQQQPLRPEGFEGSMPPEGEIPEEFREQYEQQFQEQFHQQFEQQFQEEYQRQLQ
                     QQSEVPQEFHEEESLPESSLLKQSFLGAVVEAMQPGLRILFPFLSLRL"
     gene            complement(1462..1704)
                     /locus_tag="VFE94_03170"
     CDS             complement(1462..1704)
                     /locus_tag="VFE94_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50119.1"
                     /translation="MGKNIFYFVVALAVLLLVGGVIFFATQQGAPSQEAQPNETLQQT
                     AGEEQQEEGLGSELNQNPGSKVPETNPLEGYKNPFE"
     gene            complement(1734..2705)
                     /locus_tag="VFE94_03175"
     CDS             complement(1734..2705)
                     /locus_tag="VFE94_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZX50120.1"
                     /translation="MKILLVQDLTRPHHGLGDEHLEKIKAVDPSLEVLAFSSTQLDEI
                     NRRIEDVDILAGTLWAIPEIHDVKKLKLIHSFSAGVEHVLTPEVKNSDVIVGNCSGIH
                     ATPIAEHVLGFLLIFTRRFYDTFQSQQKKRWEKNPKITELRGKTVLIVGLGNIGKEVA
                     RVVNGVGARVLAVDRGGEEKPEFVETLYAQDQLKEALPQADFVVLCLPLTGETRHLFD
                     MEKFRIMKNDAVLVNIARGGLVKEQDLIQALSQKVIGGAALDVTEEEPLSQESPLWEM
                     ENVVITPHHSGSSEKYMDRAVDVFCENLKAYLKGETLPNQVDKERGY"
     gene            complement(2709..3680)
                     /locus_tag="VFE94_03180"
     CDS             complement(2709..3680)
                     /locus_tag="VFE94_03180"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HZX50121.1"
                     /translation="MEDAIRNFSKQFEWEPDIEQAEQWKAVGKYIICGMGGSHLQGDV
                     FQNAYPGFDLSVHQDYGLPGWPKEVLRKTLVIASSYSGNTEETLSSFQEAVKNGYPAA
                     AISTGGELLNQVKQHGVPYIQIPDIGIQPRSALGYTFKALAKMVGRDDVVKQATQVGK
                     MLAESAATFEAQGKALAEKLHGKVPVIYASQKNYSIAYNWKIKFNETSKIPAFYNVFP
                     ELNHNEMNGFDVSDSTRELTKPFFFLFLRDGEDHPRIQKRMEVLEKQLYQRGLPVINC
                     ELTGNSRLEKIFSSLLFADWVALYLAQHYGRDPEQVPMIEEFKKLIG"
     gene            complement(3970..4215)
                     /locus_tag="VFE94_03185"
     CDS             complement(3970..4215)
                     /locus_tag="VFE94_03185"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HZX50122.1"
                     /translation="MATKKFKQEFRARTVGFIATALGLVVGLAWNDAVKTFIETIFPI
                     KGDTILLKFFYAVILTFIVVSVIILLMRFVEDEGDKK"
     gene            complement(4215..5441)
                     /locus_tag="VFE94_03190"
     CDS             complement(4215..5441)
                     /locus_tag="VFE94_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50123.1"
                     /translation="MEKLAEILGVPKEKLEELEKAMVAKGFESSLEKVAQQNEELVER
                     ALAELGVTSRNPEDITAALMAKAAESDEQLYKFLGLDPANLDFHKIAQTAREIATERE
                     GWFLKKEYGRQILRERQPQATLDFLGYKDTEELLQKQDVTEIFSALRFLETDSWMHKT
                     FDVAYTKFTPADFEHRPIEIQVLGKQWEEVAKKYVAKKHHNVSHLKEFGVIFLNPIAQ
                     TERGKFVRDFALLLHYFHEIAFYSRLFQKYAQQDNFQQNFIALLRGDVKEASEVKPGE
                     WLLVQRYLWKENPQDPRLFLPHVNPESMHWRKAEQDLVAFGKKYEEINLEFWDQYWSV
                     AEFFSGEQGTRTLVSFDLEDVAMMVAHKSEGKDIQLTYHQHEALWNRLFAAYVGGYDK
                     VEGHIIKNMEHGIIRL"
     gene            complement(5460..5720)
                     /locus_tag="VFE94_03195"
     CDS             complement(5460..5720)
                     /locus_tag="VFE94_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HZX50124.1"
                     /translation="MVDIQVSERFKKNYRKLPEKIKQRAKEREVVFRANPFDSILKTH
                     KLSGKEKGIWAFWINDSYRIKFIFLPNKEVLFLDIGTHKIYK"
     gene            complement(5720..5959)
                     /locus_tag="VFE94_03200"
     CDS             complement(5720..5959)
                     /locus_tag="VFE94_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50125.1"
                     /translation="MAKNLYVKDAVAVVEKEVKKKGGVVVLSLEEYRSLLERAVPLYQ
                     LHGKEAEEIDMLFKEGMAEYKAGKTTKIKSLSDLD"
     gene            complement(6071..6730)
                     /locus_tag="VFE94_03205"
     CDS             complement(6071..6730)
                     /locus_tag="VFE94_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50126.1"
                     /translation="MSKPLVIGTIVAVVGIFALGGFLLWQNVSNQVTHTPEQQTPPPP
                     VPSPQPQAIDNQQSEIDTSNWQTYRSDEFGFEVKMPENWMAREFEVKIDLGIFNLPEY
                     YAVSVSKIENNEGMTNREYAEQWFESRKDLLSDYEVSSTGAAEVSVGEYVGYKIQGFH
                     TGIEGESTIDVIFVELPNDDSIYQVQFPTGEFQTGQHIISPEENYQIANQILSTFRFV
                     E"
     gene            complement(6758..7864)
                     /locus_tag="VFE94_03210"
     CDS             complement(6758..7864)
                     /locus_tag="VFE94_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZX50127.1"
                     /translation="MDSAIESSNENQIADTATSSSEDSTEYEHKQCEYGEFSEDLYLD
                     TYVVKPGDTLLSIANHQLGSTARIDEIINANKFRDAPYDSLGSSDTIQAGWELYLSTV
                     FLPPSSGNLDAQQGKLIIADSGGYKGRMLLQNDVNYNPVNRAPLIFAESVAFFNDNSF
                     QEGDCIMAVGDRDARSDTGIIALAPQGDPSVFKAPVAVYEKHEVELPLETLECRYGFF
                     SSQEVLRSYTVRPGDTLYSVANGQVGDISRVPELREINTGRYTSLQQSDFIEIGWELL
                     LPPSWTDDFTGLYSGTVGFFGGEDKESFWIEANLTDHGASSSRFLKTPEMRYFGENTF
                     VRGDCVVTFHAGGARALGIAPQSEDYLRYFKYGE"
     gene            complement(7960..8955)
                     /locus_tag="VFE94_03215"
     CDS             complement(7960..8955)
                     /locus_tag="VFE94_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZX50128.1"
                     /translation="MFDVITIGSAVRDVFARTKDIKAIKDKKFAVGQAACFPLGSKLN
                     LDEVQFSVGGGAINTAVTFANLGYKVAAIAKVGADPEGSHILDKAAAYGIHCDFIGQD
                     KEHFTSFSFILSLPDGSRTVFRYKGASWHLQDSHIPWMDFRAKWLYINHLAGNSGNIL
                     PKLLERAKSKGMQIAWNPGTTQLEKPAELKKYMNYADLFIVNQEEAATITGIPYQKKK
                     QIFKKLDELVHGKVIMTKGAKGVDVSDGKTLWSAGVLPLAKDRIVDRTGAGDAFGSGF
                     VAAVMQKPSNVEHAIQFASANATGVLTQWGANHGLLKRGDSPFKFGKLEIKKTNV"
     gene            complement(8964..9470)
                     /locus_tag="VFE94_03220"
     CDS             complement(8964..9470)
                     /locus_tag="VFE94_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50129.1"
                     /translation="MPKIVIVAVIIGLIAVTGVLVWLIVGGGANGPEQPGEQNPTYPL
                     EPPQIILSYQGQEYRGVQGSYCWPAEEGVNPLCADTAFPNPQESIQVAAGDTVDVAIQ
                     NIEVAPIELGIGLFTSPEDATPTLVEVVSGLQSSFAPNAPPGEYIVIVSARWDVGDIY
                     YGFKINVQ"
     gene            complement(9475..>10354)
                     /locus_tag="VFE94_03225"
     CDS             complement(9475..>10354)
                     /locus_tag="VFE94_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZX50130.1"
                     /translation="LGATKKDMVVLVADTMESSRAHFFGEKFPERLIQVGVAEQNLTG
                     IAAGLAYEGKVPYVVTYAAFLLGRAWEPIRTTIAYPENHAVYVSSHAGLATGQDGPTH
                     QMTEDISITRCLPNFTVVAPADFEQAKKAVKAAYDLKGPVYLRGVREGTPNFTTSNTP
                     FEVGKAQVLREGKDVTAIGHGYMVYWLLQIAEELKDKVSIEVINLHTIKPFDEDTILR
                     SAKKTKRVFCAEDHNVIGGMGSAVAEFLGENCPVPIKLHGVYDTFTESGSPKDLWKKY
                     RLDKDGVRQVLMGFIK"
BASE COUNT         2485 a         2802 c         2134 g         2933 t
ORIGIN      
        1 gatacctcgg caaaagctgc aagagctttt tgcacctcct cttcctgcca gcccgcttct
       61 ccgagcaccc gttggatatc gcctcggctc tgcccctttt ccagggcttc ttttacaaat
      121 gtatagagtt cgtttgtcat agtcgtagag aaagaaacgg aaataaaatt cttaaccctg
      181 gctgcatcgc ctcgaccact gcgccaagga aactttgttt tagtaagctc gactctggga
      241 gagattcctc ttcgtgaaat tcttgaggga cctcactttg ttgttgaagc tggcgctggt
      301 actcttcctg aaattgttgc tcaaactgct ggtgaaactg ttcctggaac tgctgctcat
      361 attgttctcg aaattcctct gggatttccc cttctggggg catcgaccct tcgaaacctt
      421 cggggcgtag gggctgctgt tgtggaaagc actcctcgcg gatccgctgt tcttgttctg
      481 cggttggagg cccctgaagc tcccgtgtat ctccaagaat cctttgcacg cagtcggcag
      541 cttctggcgg tacctcacca cctacctgtt cttgaatatt ctgctggata atattgggga
      601 gatgagtttc aaaacactgg cgcatatgct ctccgagttc ccggcttggc aaaaatgttc
      661 cggccccgat ttttgcgagc acgtcttcgc caacggtctc tttcaggcac tgggcgactt
      721 cggggggcgc actttggaga ccgcgtttga acatctcgat tccctcctgc atctgctgca
      781 actcttcttg cggaatgaga tcgtgctcct ttgcaaattc gaaacaaact ttctggtttg
      841 ccggggcatt gcaaaaggct tcgcattcct cacggcctcg gcaatcaccc gggccgcgcc
      901 cgccggtttt acggaacatc tctgcttctt gtacgcttat gaatcctgct ttctcaaaaa
      961 acgcggcgca ttcttcaatg tgaccatctt gcgaacaata cgcctcgcac tcctctcgcg
     1021 atcggcagcc gcccggcatt tcgccccgcg ccatcaacgg ggctacacgc ttggcatctt
     1081 ccaattcctc cggactcata aacccggcgg cttcggcaaa cgcaatacac tcttcaatat
     1141 gcgcgccgtc tttacagtac acttcgcact cttccttgtt gcggcacccg cccggaagct
     1201 gtgcgccctg ccgcaaagcc gacacaacct tttttgcttc ctccagttca ccgcccttca
     1261 ttaagccgtg ccgctctgca aaattgacgc attgttctat atgttcaact tcatcgcagt
     1321 aggcatggca ctcttctttg ctttggcaat tcccaagctc cgcaacggga aactcaacgg
     1381 ctggctcttc ttgggcaaaa acggcgtaag ctccggccaa agccacaaaa acaacggaag
     1441 atatcaataa aattctcatg attattcaaa aggattttta tatccctcaa gagggtttgt
     1501 ttccgggacc tttgagccag ggttttggtt aagctcgctg cccaaccctt cctcttgctg
     1561 ttcttcgcct gcggtctgct ggagagtttc gtttggctgc gcctcttggc ttggagctcc
     1621 ttgttgtgtt gcaaaaaata ttacccctcc tacaagtaac aacaccgcca aggcaacaac
     1681 aaagtaaaaa atattttttc ccatattttt aaatacttcg attctgctca gtactagtat
     1741 cccctttctt tatcgacttg attagggaga gtctctccct taaggtacgc cttgagattt
     1801 tcgcaaaata cgtcgaccgc gcggtccata tatttttcgg aacttccgga gtggtgcggc
     1861 gtaatgacta cattttccat ttcccacaac ggactttctt gggaaagcgg ttcttcttcg
     1921 gtaacgtcaa gcgcagctcc cccaatcact ttttgcgaca gcgcctggat caagtcttgc
     1981 tcctttacaa gcccaccgcg cgcaatattt acaagcaccg cgtcattctt cattatacgg
     2041 aatttctcca tgtcaaacaa atggcgggtt tccccagtta agggcaagca cagtaccaca
     2101 aagtctgcct gggggagcgc ttctttcagc tggtcttgag cgtagagcgt ttcgacgaac
     2161 tctggctttt cttccccccc gcggtcaacc gccagcacgc gggctcccac cccgtttact
     2221 acccgggcaa cttcctttcc aatattcccc aacccgacaa tcaaaacagt tttaccccga
     2281 agctctgtaa ttttgggatt tttctcccat ctcttctttt gctggctttg aaaagtgtcg
     2341 taaaaccttc gggtaaaaat cagcaaaaat cccaatacat gctcggcgat gggcgtagca
     2401 tggattccag aacaattccc tacaattaca tcggagtttt ttacttccgg cgtcaataca
     2461 tgctctacgc cggccgaaaa ggagtgaatt agtttgagct ttttaacgtc gtggatttcg
     2521 ggtatggccc aaagagttcc ggcaagaatg tctacgtctt ctatgcggcg gttaatctcg
     2581 tctaattgcg tagaagaaaa tgccaatact tccaacgatg gatctactgc tttaattttt
     2641 tccaaatgct catctccaag cccgtgatgc ggacgcgtaa gatcttgcac caaaagaatt
     2701 ttcatgagtt agccgatgag ctttttaaac tcttctatca taggcacttg ctccgggtcg
     2761 cggccataat gctgtgccaa atacaacgcg acccaatcgg caaaaagcaa ggaagaaaag
     2821 attttttcca ggcgcgaatt tcccgtaagc tcacaattaa taaccggcaa acctctttgg
     2881 taaagctgtt tttccagcac ttccatgcgt ttctgaatac gaggatggtc ctcaccatcg
     2941 cgcaaaaaca aaaagaaaaa cggcttggta agttcgcgtg ttgaatctga aacgtcaaac
     3001 ccattcattt cgttgtggtt gagctctggg aacacattat aaaaagccgg gattttactg
     3061 gtttcgttga acttaatttt ccagttgtag gcaatagagt agtttttctg ggaggcgtat
     3121 ataacaggca ctttcccgtg caatttttcc gctaacgcct ttccctgggc ttcaaaagtt
     3181 gctgcgcttt ctgcgagcat ttttcccacc tgggttgcct gttttactac gtcgtcgcgc
     3241 cccaccattt tcgccaaggc cttaaaagta tagcccaagg cggacctcgg ctgaatgccg
     3301 atatccggga tttggatata tggcacgcca tgttgcttaa cctggttaag caactcgccg
     3361 ccagtggaaa tggcggctgc cgggtagccg ttttttaccg cctcttggaa cgaactcaac
     3421 gtttcttcgg tgttcccaga atacgagctt gcgataacaa gggttttccg gagtacctct
     3481 ttgggccagc ctggcagccc ataatcctgg tgaaccgaca agtcaaaacc agggtacgca
     3541 ttttgaaaca cgtcgccctg caaatgcgag ccgcccatac cgcatataat atacttgccc
     3601 acagctttcc actgctcggc ttgctctatg tctggctccc actcaaactg cttggaaaaa
     3661 ttccggattg cgtcctccat agcaaaatgt ccgtttataa aactgttatc atccaacctg
     3721 ctgcggccaa acccaatacg cttacaatac ccacaaattt ccaattcgtt tgcggatcgt
     3781 ttaattgcat tttcatgata taaaatgttt gcgatattac ttatatataa acgcgacctt
     3841 tccttttttg tatcatgctt tagttcgcta gaaggctgat aaccgcaatt acaagcgtaa
     3901 acaccaaaac gataaatcct aaaactatgg gaaattgggt tcgcaggttc atacactatt
     3961 actattgcat tattttttat cgccctcgtc ttccacaaac ctcatgagta aaattataac
     4021 acttaccaca ataaatgtaa gtatgaccgc atagaaaaac ttcagcagga tagtatcgcc
     4081 ctttatgggg aaaatagttt ctatgaaagt ttttaccgcg tcgttccacg ccaaacccac
     4141 caccaaccca agcgccgtgg ctataaaccc cacggttctg gcgcgaaatt cttgtttaaa
     4201 cttttttgtt gccattacaa ccgtattata ccatgttcca tattttttat aatatgccct
     4261 tccactttgt cgtagccgcc cacgtacgcg gcaaacaaac ggttccacag ggcctcgtgc
     4321 tggtggtagg taagctgtat gtctttgcct tctgacttgt gtgctaccat catggcaacg
     4381 tcctccagat caaaacttac aagagtgcgc gttccctgtt cgccgctgaa gaactctgcc
     4441 accgaccagt actggtccca gaactccagg ttaatttctt cgtacttttt gccaaaggcc
     4501 accaaatcct gctctgcttt tctccaatgc atggactccg ggtttacgtg cggcaaaaac
     4561 aaacgcgggt cttgcgggtt ttctttccac aaataacgct gcaccagcag ccactcgccg
     4621 ggcttaacct cgcttgcctc ttttacgtcg ccgcgcagca aagcaataaa gttttgctgg
     4681 aaattatctt gctgggcgta tttttggaat aaacggctgt aaaacgcgat ttcgtggaaa
     4741 tagtgcaaca ataaggcaaa atcgcgcaca aatttgccgc gttcggtttg cgcgatagga
     4801 tttaaaaata taacgccaaa ctcttttaaa tggcttacgt tatggtgctt ttttgccacg
     4861 tattttttag ccacctcttc ccactgcttg cctagcactt gtatctcgat aggccggtgc
     4921 tcaaagtcgg ccggagtaaa tttggtgtag gccacgtcaa aggttttgtg catccagctg
     4981 tcggtttcca aaaaccgcag ggcgctaaaa atttcggtta catcctgctt ttgcaaaagt
     5041 tcttcggtat ctttataccc aagaaaatcc agcgttgcct ggggctgcct ctcgcgcaaa
     5101 atctgccggc cgtattcttt ttttaagaac cagccttctc tttccgtggc aatttcgcgc
     5161 gcggtttgcg cgattttatg aaaatccaaa ttcgcgggat ccaaacccaa aaatttgtac
     5221 agctgctcgt cgctttccgc cgcttttgcc ataagcgcgg ctgtaatatc ttcagggttt
     5281 ctgctggtta caccaagctc ggctaatgcc cgctctacca gctcctcgtt ctgctgcgcc
     5341 accttctcca acgatgattc aaaccctttc gccaccatag ccttctccaa ttcttctagt
     5401 ttttctttgg gcacccccaa aatctccgca agtttttcca tagtattatt ccttttattc
     5461 tatttataaa tcttatgcgt gccaatatcc aaaaataaaa cttccttgtt tggaaggaag
     5521 ataaatttaa tacggtacga atcgtttatc cagaaagccc aaatgccctt ttctttccca
     5581 gaaagtttat gtgtcttgag gatggaatcg aaaggattgg ctcggaaaac cacctccctt
     5641 tcttttgcgc gttgcttgat tttctcggga agcttgcgat aattcttctt aaaacgttcc
     5701 gaaacttgaa tgtctaccat taatctaaat cactcaagga ttttattttc gtggtctttc
     5761 ccgccttata ctctgccatt ccttctttga aaagcatatc tatttcttct gcctctttgc
     5821 catgcaactg atataagggc accgcccgtt ctaaaaggct gcggtattcc tccaaactca
     5881 aaaccaccac ccctcctttt ttcttaactt ccttctcaac aaccgccacc gcgtctttta
     5941 catatagatt cttcgccata ataactttac tttagcagat tttcccgcct tctgcaactc
     6001 cctttggctc ccaaaatctc cgcaattttt ttccataaaa cttgtagtaa agtttaacct
     6061 taccttacta ttactccaca aaccgaaacg tggagagaat ttggtttgct atttggtaat
     6121 tttcctctgg actaataatg tgctgccctg tttgaaattc acccgtggga aactgaactt
     6181 gatagattga atcgtcgtta ggcaactcta cgaaaatcac atcgatagtt gactcgcctt
     6241 ctatgccagt atgaaatcct tgaattttat aacccacata ctctcctacg cttacttctg
     6301 cagcacccgt tgatgagact tcgtaatcgg aaagcaagtc ttttctactc tcaaaccatt
     6361 gctctgcgta ctctctattg gtcattccct cattattttc aatcttcgag accgaaactg
     6421 cgtagtactc cggaagatta aatataccta gatcaatttt tacctcgaat tcccttgcca
     6481 tccaattttc tggcattttc acctcaaacc caaactcgtc ggagcggtag gtttgccaat
     6541 ttgacgtgtc tatctcggat tgttgattgt cgattgcttg aggctggggt gacggcacgg
     6601 gcggcggtgg ggtttgctgc tcgggcgtgt gagtaacctg gttactcaca ttctgccaca
     6661 aaagaaatcc gccgagggcg aagatgccca caactgctac aattgttccg ataaccaatg
     6721 gtttgctcat aggtttaagg ctaaattatt tttacattca ctctccatat ttgaaatacc
     6781 gtaagtaatc ttcgctctgc ggcgcaatcc ctaacgctcg agctcctcct gcatggaacg
     6841 taaccacaca atctcccctt acaaaagtat tttccccaaa atacctcatt tccggagttt
     6901 ttaaaaatct tgatgaactc gccccatgat cggtcaagtt agcttctatc caaaagcttt
     6961 ctttgtcctc gccaccaaaa aagccaaccg ttcccgaata aagcccagta aagtcgtcag
     7021 tccacgatgg aggcaaaagc agctcccatc ctatctctat aaaatcactc tgttgcaaac
     7081 ttgtataccg tcctgtgttt atctctcgca gttctggcac ccgtgagata tccccaacct
     7141 gtccgttcgc aaccgagtac aatgtatctc caggcctcac cgtgtagctt cgcaaaacct
     7201 cttggctact aaaaaatcca tacctgcatt cgagagtttc aagaggaagt tcaacttcgt
     7261 gcttttcgta cacagcaacg ggcgctttaa aaacagaggg gtctccttgc ggcgccagag
     7321 ctataattcc ggtgtctgaa cgcgcatcac gatcccctac tgccattatg caatctccct
     7381 cctgaaaact attatcattg aaaaacgcaa cactctctgc aaagatcaag ggcgcgcggt
     7441 taacaggatt gtaatttacg tcattctgaa gaagcatcct gcctttgtac ccgccggaat
     7501 cagcaatgat taattttcct tgctgcgcat caagatttcc ggagcttgga ggaaggaaaa
     7561 cagtagacaa atacaattcc caacctgcct gtatagtatc actactcccg agtgaatcgt
     7621 aaggagcgtc acgaaatttg ttcgcgttga tgatctcatc aatgcgtgcg gtactgccaa
     7681 gttgatggtt tgcaatagaa agtaaggtgt cgcccggttt taccacgtac gtatccaggt
     7741 agagatcttc cgaaaattct ccgtactcac actgtttatg ttcatattcc gtactgtctt
     7801 ccgaggagga cgtcgccgta tcggctattt ggttctcgtt cgacgactct atagcagaat
     7861 ccatccgtgc aagttgctgc cgcaagacaa agccggcaac gagaactaca agaaggattg
     7921 cgattgtacc gagggtaaca agatttagtt cgcgacgagt catacattcg ttttctttat
     7981 ctcgagtttg ccgaatttaa agggagagtc gccgcgcttt aacaggccat ggttggcgcc
     8041 ccactgggta agcacgcccg tggcgtttgc cgaggcaaat tgtatggcat gctctacgtt
     8101 gcttggcttt tgcattacag ccgccacaaa cccggaacca aacgcgtcgc cagctcccgt
     8161 acggtctaca atcctgtctt ttgccaaagg aagcacgccg gcgctccaca gcgttttgcc
     8221 atcggacaca tctaccccct ttgctccctt ggtcataatt actttgccat gcaccaattc
     8281 atccaacttt ttaaaaatct gctttttctt ttggtatgga attccggtta tggtggccgc
     8341 ttcttcctgg tttacaataa acaagtcggc atagttcatg tactttttta gctcggccgg
     8401 cttttccagc tgggtggtgc ctgggttcca cgcaatttgc attcccttgc ttttcgcccg
     8461 ctctaacaac ttgggcaaaa tgtttccgga atttccggcc aaatggttta tatacagcca
     8521 ctttgcccga aaatccatcc agggaatatg ggaatcttgc aaatgccacg aagcgccttt
     8581 gtaccggaac acggtgcgcg aaccgtcggg caaagaaaga ataaacgaaa atgacgtaaa
     8641 atgttctttg tcttgcccga taaaatcgca atggatgccg tacgcggccg ctttgtctaa
     8701 aatatggctg ccttcgggat ctgcccccac tttggcgatt gccgccacct tgtatcccaa
     8761 attggcaaag gtaacggcgg tgtttatggc tcccccgccg acggaaaatt gcacctcgtc
     8821 caaattgagt ttggagccca aaggaaaaca cgcggcttgc ccaaccgcga attttttgtc
     8881 ttttatggct ttaatgtctt tagtgcgggc aaacacgtcc ctaaccgcag accctattgt
     8941 aattacgtca aacatacatt cttttactgg acattgattt taaagccgta atatatgtcg
     9001 cccacatccc aacgggcaga aactataaca atatattcgc cgggcggcgc gtttggcgca
     9061 aacgaactct gcaaaccgga aacaacttcc acaagcgtgg gcgtcgcatc ttctggcgac
     9121 gtaaacaaac cgattcccag ttctataggc gcaacttcta tgttttgtat ggcaacgtcc
     9181 acggtatcgc ccgcggcaac ctgaatactt tcttgcgggt ttggaaacgc ggtgtccgca
     9241 cacagaggat ttacgccttc ttccgcaggc cagcagtaac tcccttgtac tccccgatat
     9301 tcttggccct ggtaactcaa tataatttgc ggcggctcta aaggatatgt tgggttttgc
     9361 tctcctggct gctcgggccc attcgcgcca cccccaacaa taagccaaac caaaacaccg
     9421 gtaacagcaa taagcccaat tattacagca acaattacaa tttttggcat attcttactt
     9481 tataaaaccc ataagcacct gccgtacccc atccttatcc agcctatatt tcttccataa
     9541 atccttgggg ctgcccgatt ccgtgaacgt atcatacacg ccgtgcagtt ttatgggtac
     9601 cgggcagttt tccccaagaa attccgccac cgcgctgccc ataccaccaa taacattgtg
     9661 atcttcggcg caaaacacgc gctttgtctt ttttgccgag cgcagaatag tgtcttcgtc
     9721 gaacggcttt atggtatgca agttgattac ctcgatggaa accttgtctt ttaactcctc
     9781 tgcaatttgc aacaaccaat acaccatata cccgtgccca attgccgtaa catctttgcc
     9841 ttcacgcaga acctgcgctt tcccaacttc aaaaggcgtg ttgcttgtag taaaatttgg
     9901 cgtgccttcg cgcactcccc gcaagtacac cggccctttc aagtcgtagg cggcttttac
     9961 ggcttttttc gcttgttcaa aatccgcggg cgcaactacg gtaaagttgg gtaggcagcg
    10021 tgtaatggaa atgtcttccg tcatctgatg agtggggccg tcctgccccg tagccaagcc
    10081 cgcgtgcgag cttacgtata ctgcgtggtt ttcagggtac gcgatggtgg tacgaatagg
    10141 ctcccatgct ctgcccagta aaaaagcggc atacgttacc acatagggca ctttaccctc
    10201 gtacgccaac ccggccgcta tgccggttaa gttctgctct gccacaccca cctgtattag
    10261 gcgctcgggg aatttctcac caaaaaaatg cgccctggaa gattccatgg tatctgccac
    10321 cagcaccacc atgtcttttt tggtagcgcc cagc
//
LOCUS       DASRUP010000078         4807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1771651, whole genome shotgun sequence.
ACCESSION   DASRUP010000078 DASRUP010000000
VERSION     DASRUP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771651"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VFE94_03230"
     CDS             complement(<1..1107)
                     /locus_tag="VFE94_03230"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HZX50131.1"
                     /translation="MKIVFTGGGTGGHILPIIAIVRELKSTYKDTDLELFYMGPEDKF
                     GDVLLSQEGVQTFNVPAGKLRRYWGFAAFFQNLADLFIWIPLGIAKALKVLFFISPDL
                     IFSKGGYGAIPATVAGRLFLRIPVFLHESDIVPGKANLAAQRFALEVFTSFPQTRNFP
                     PKKVLLVGNPIRTRLLSGSVQEAHQLFQLVGGKPVLLILGGSQGAKRVNDMLLSVLND
                     ALSQFEIIHQTGYANFKEAEEEAAVAAAEENLPYYHPIPFLNENEIRHAYAAADFIVS
                     RAGSGTIFEIAALGKPSILVPLPESAQNHQVENAYAYAQTGAALVLEEPNVKPHFFLE
                     RIRNIIFAQEEMAHMSKAALQFAKPEAARIIANYI"
     gene            complement(1118..2221)
                     /gene="ftsW"
                     /locus_tag="VFE94_03235"
     CDS             complement(1118..2221)
                     /gene="ftsW"
                     /locus_tag="VFE94_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZX50132.1"
                     /translation="MQQRNSPDLPLLSLAVALLFVGMLILASVSVSFSLQNTGTTFYY
                     LNHQLLLGLLPGLVAATIAFFTPLPFIRKMSLWFLLFCIFLLALVFVPGIGSGETGGA
                     HRWLSAGPFSFQPAELLKVAFVLYLASWLSAKKKRRQENTKETLLPFAAMMGVVSLFL
                     ILQPDIGTLAVIAATGLTMYFLAGTPLWHAATLVGIGLLALGGLVLIAPYRFSRFAVF
                     LNPSLDPLGQGYQVKQALIGIGSGGLAGAGLGLSFQKFGVLPEPISDSIFAVFAEETG
                     FLGGLLLIGLIVAFAWRGLIVSKRVLSDQFASLAAAGIVSWIFLQSAVNIAAMIGLLP
                     LTGIPLPFVSYGGSALFSELLAVGILLNISKEA"
     gene            complement(2199..3506)
                     /gene="murD"
                     /locus_tag="VFE94_03240"
     CDS             complement(2199..3506)
                     /gene="murD"
                     /locus_tag="VFE94_03240"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZX50133.1"
                     /translation="MLIAELKDKEILILGFGREGQDSFLFLRTKFPQKSIGIADQKEF
                     DQLAPETQKLLNRDRKVVLYMGDSYLAQVNKYDVVIKSPGIPRGLVLPLVKKSQVLCS
                     QTSLFFDNCPGTIVGITGTKGKSTTASLIYEVLKQGKIKAHLIGNIEQPVFQFLEAAT
                     KDDVYVYELSSFQLEGLQKSPHIAVFLNLYPEHLDHHGDFKTYVQAKASIAKFQTPED
                     YFIFNETDPEVSRIAKLTKAAKIPFTPTRENGASFVAATEPVFLVAELFDISKEKAQK
                     AIAVFKPLAHRLELVGEKRGITFYNDSLATIPEATIAALDALGENVATLIAGGFDRGV
                     PMGKLAQRIAESNIKTLVLFPTTGEKLWKEIEKAQPNGELPKHFFANTMADAVEICYR
                     ETPKGKICLLSPAASSFNMFRDYKERGEEFKKFVKLYATKKLS"
     gene            complement(3515..>4807)
                     /locus_tag="VFE94_03245"
     CDS             complement(3515..>4807)
                     /locus_tag="VFE94_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50134.1"
                     /translation="TFTYKGYSYEVKDGDLEMAFDFRLGQTRFSPRVVIKNVPAKSWL
                     LSPTKQPILENLVFHLGLAEMPSYWKVTASPRIIVEAGNLNKTQINWWHDLFTKGMGE
                     YFYKNNIDFRSKNFLTIESGGPAQRSFGEGGGVVFSKKTSNKTLVPIGGGKDAVVTYE
                     ILKRAKQAIKPFVLNPTNAQKALLRKMKEGSAITVQRTIDPKLLELNREGYLNGHTPF
                     SSYLAFLTTLCAALFDYKYIALSNERSSNEGNVQYLGHEINHQYSKSFEFEKKFRIYS
                     KKYLTENIEYFSFLRPLYELQIAKLFAQYPQYFGTFLSCNEAHKTHSGRVKATGKWCG
                     ACSKCLFVFLVLSPFAREKTLRIFKKDLLQDKELKPLLDELTGKRGFKPFECVGTTKE
                     SRAALELQTRKDHPLLHAWNNKHFLPRNLEKALRSMLQ"
BASE COUNT         1235 a         1160 c         1135 g         1277 t
ORIGIN      
        1 gatatagttg gcaataattc gggcggcttc cggctttgca aattgcaaag cagctttcga
       61 catatgcgcc atttcttctt gtgcaaaaat aatgttgcgt attcgctcca agaaaaagtg
      121 cggcttcacg ttgggttctt ccaacaccaa agcggccccg gtttgggcgt acgcatacgc
      181 attttctacc tggtggttct gtgcggattc tggaagcgga accaatatgc tcggttttcc
      241 gagagccgcg atttcaaaaa tggttcccga tcccgccctg ctcacaataa agtccgccgc
      301 cgcgtacgcg tggcgtattt cgttttcgtt gaggaatgga atggggtggt aatacggcag
      361 gttttcttcc gcggctgcta cagcagcttc ctcttctgct tctttgaagt ttgcatagcc
      421 tgtttggtgg ataatttcaa actgtgaaag cgcgtcgttc agcacgctca aaagcatatc
      481 gtttacccgt ttggcccctt gggaaccgcc aagtatgagc aaaacaggtt ttcctcccac
      541 aagctggaaa agctggtgcg cttcctggac agaacccgaa agcaggcgcg tgcgaatggg
      601 gttgcccaca agaagcactt ttttgggcgg gaagtttctt gtttgcggaa acgacgtgaa
      661 cacctcgagg gcaaatcgct gtgcggcaag attggctttt ccgggcacaa tatcagattc
      721 gtgcagaaat accggaatgc gcaaaaacag ccgtccggca acggtggcag gaattgcccc
      781 gtatccgcct ttgctaaaaa taaggtctgg cgagatgaaa aagagaactt tgagagcctt
      841 tgctatgccc aagggtatcc agataaacaa gtcggccaga ttttggaaaa acgcggcaaa
      901 tccccagtat ctgcgcagtt tccccgcggg cacgttaaaa gtttgcacgc cttcctggga
      961 aagcagcacg tcgccgaatt tatcctctgg gcccatatag aacagctcca ggtccgtgtc
     1021 tttataggtt gacttgagct cccgtacgat agcgataata ggaagaatat ggccccctgt
     1081 tccgcccccg gtaaaaacaa ttttcataca ctttgaatta ggcttcttta gatatattaa
     1141 gcaaaattcc cacagctagc aactctgaaa acagcgcaga ccctccgtaa cttacaaagg
     1201 gcaaaggaat gcctgttaaa ggaaggagcc ctatcatggc cgcaatgttt accgcagatt
     1261 gcaaaaagat ccaggatacg attcccgcgg cagctaaact cgcaaactgg tctgaaagaa
     1321 cccttttgga tacaataagc cctcgccatg caaaagcaac gatgaggcct ataagcagca
     1381 gccctcccaa gaagccggtt tcttcggcga acacggcgaa aatggaatct gaaatgggtt
     1441 ctggcagtac gccgaatttt tgaaagctca acccaaggcc ggctcccgca agccccccag
     1501 acccaatgcc aatgagagcc tgttttacct gatacccctg gcccaaagga tcgagcgacg
     1561 gattaagaaa caccgcgaat cggctaaacc ggtaaggcgc aataagaacg agcccgccca
     1621 aagccaacaa tcctattcct accagtgttg ccgcgtgcca aagcggagtt cctgcaagaa
     1681 agtacatggt aagcccggtc gctgcaatta ccgcaagagt tccaatgtct ggctggagaa
     1741 taagaaaaag gcttaccacg cccatcatag cggcaaaagg cagcagggtt tcttttgtat
     1801 tttcctgcct gcgttttttc ttggcagaaa gccacgaagc cagatacaga acaaaagcca
     1861 ctttgagaag ttccgcaggc tggaacgaaa agggtcccgc cgaaagccac cgatgcgctc
     1921 ccccggtttc gccgcttcca atgcccggga caaacaccag cgcaagcaag aaaatgcaaa
     1981 acaaaagaaa ccaaagactc attttcctaa taaaaggaag cggggtaaaa aacgcgattg
     2041 ttgctgccac gaggcccggc aaaagcccaa gcagcagctg atggttgagg taataaaacg
     2101 tagtgccggt attttgcaaa gaaaaagaaa ccgaaacgct ggctaaaata agcatgccca
     2161 caaacaaaag cgcaacagcc aggcttagca aaggcaagtc aggagagttt ctttgttgca
     2221 tagagtttta cgaatttttt aaactcttct cctctttctt tgtagtcgcg aaacatatta
     2281 aagctcgagg ctgcgggcga caacaaacat attttgccct ttggtgtttc ccggtaacaa
     2341 atttccacgg catctgccat ggtgtttgca aaaaaatgtt ttggcaattc cccgtttggc
     2401 tgcgctttct caatttcctt ccaaagtttt tcgcccgtgg tcgggaaaag caccagcgtt
     2461 ttgatgttgc tttccgcaat gcgttgggcc agtttgccca tgggcactcc cctgtcgaaa
     2521 cctcctgcaa taagggtggc aacgttttct ccgagggcgt ctaaggcggc aatagtggct
     2581 tcgggaatag ttgccaaaga atcattgtaa aacgtaatgc ctcttttctc ccctaccagc
     2641 tctaaacggt gcgccagagg tttaaaaaca gcaatcgcct tttgcgcctt ttctttggat
     2701 atgtcgaaaa gttcggcaac aagaaagacg ggttctgttg ccgcaacaaa cgaagccccg
     2761 ttttctcttg tgggagtaaa aggaattttt gctgctttgg taagttttgc aatgcgcgaa
     2821 acttcaggat ctgtttcgtt aaaaataaag tagtcttccg gggtttggaa tttagcgatg
     2881 ctggctttgg cttggacgta ggttttaaaa tctccgtggt ggtctaaatg ctcgggatac
     2941 aaatttaaaa acacggcaat gtgcgggctt ttttgcagtc cttcgagctg gaaactcgaa
     3001 agctcgtata catatacatc atctttggtt gcagcttcca agaactgaaa gaccggctgc
     3061 tcaatattgc ctattaagtg ggcttttatt tttccctgct taagcacttc gtatataagc
     3121 gaggctgtgg tgcttttgcc tttggtgcct gttattccaa caatggtgcc ggggcaattg
     3181 tcaaaaaata agctggtctg gctgcagagc acctgggatt ttttgacaag aggcagcacg
     3241 agccctcgcg gaattcccgg agacttgatt accacatcgt atttatttac ctgggccaaa
     3301 tagctgtcgc ccatatacag cactaccttg cggtctcggt taagcagttt ttgggtttcg
     3361 ggcgccaact gatcaaattc tttctggtcg gcaatgccaa tgcttttttg ggggaatttg
     3421 gtgcgcaaaa acaaaaaaga gtcctgccct tcgcggccaa acccgagaat cagaatttcc
     3481 ttgtctttga gctctgcaat cagcatgaat gagcttattg tagcatagat cgcagagcct
     3541 tttccaggtt gcggggtaga aagtgcttat tgttccaggc atgcagcaaa gggtggtctt
     3601 tccgtgtttg caactcaagt gctgccctgc tttctttggt agtacctacg cactcaaaag
     3661 gtttaaagcc ccttttgccg gtgagttcat ccaaaagggg tttgagctcc ttgtcttgca
     3721 agagatcttt tttgaatatg cgaagcgttt tttcccgggc aaacggtgaa agcaccaaga
     3781 atacaaatag gcatttcgag cacgccccgc accatttgcc tgtagccttc actcttcccg
     3841 aatgtgtttt gtgggcttcg ttgcagctta aaaacgtgcc gaaatattgc gggtattgcg
     3901 caaacagttt tgctatttgc agttcgtaga gcgggcgcaa aaaactaaaa tattctatgt
     3961 tttctgtgag atactttttg ctgtagatgc ggaacttttt ttcaaactca aacgatttgg
     4021 aatattggtg gtttatctcg tggcccaaat actgcacgtt cccttcgttt gagctcctct
     4081 cgttagaaag cgcaatatat ttatagtcaa acaatgctgc gcacagcgta gttaaaaaag
     4141 ccaggtacga ggaaaacggc gtgtggccat tgaggtatcc ttcgcggttt aattccagaa
     4201 gttttgggtc tatggttctt tgaacggtaa tggcgctccc ctctttcatc tttcgtaaaa
     4261 gcgctttttg cgcgttggta ggatttaaaa caaaaggttt gatggcttgc ttagcgcgtt
     4321 tcaaaatttc gtacgtaacc accgcatctt tccctcctcc aatgggtaca agtgttttgt
     4381 tcgatgtttt ttttgagaat accactcccc caccttcgcc aaagcttcgc tgggcaggcc
     4441 caccggattc tattgtaaga aaattcttgc tgcgaaaatc tatgttgttt ttataaaagt
     4501 attctcccat cccctttgta aagaggtcgt gccaccagtt tatttgagtt ttgtttaagt
     4561 ttccggcctc cactataatt ctcggcgatg ccgttacctt ccagtagctc ggcatttccg
     4621 ccagccccaa atgaaacacc aaattctcca gtattggttg cttagtcggg ctaagcaacc
     4681 agcttttcgc cggaacgttt ttgataacca cccgtggact aaacctggtt tggcccagcc
     4741 taaagtcaaa cgccatttcc aagtcgccgt ctttcacctc gtatgagtat cctttataag
     4801 taaatgt
//
LOCUS       DASRUP010000079         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1933515, whole genome shotgun sequence.
ACCESSION   DASRUP010000079 DASRUP010000000
VERSION     DASRUP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933515"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..2206
                     /gene="thrS"
                     /locus_tag="VFE94_03250"
     CDS             473..2206
                     /gene="thrS"
                     /locus_tag="VFE94_03250"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZX50135.1"
                     /translation="MEKNPMENIRHSFAHVLASAVKKHYPKAELGIGPVIENGFYYDF
                     GKVKITDEDLPKLEAEMRKIAAGNHKFKKELWDPAKANRYYKKEGQPFKLDLIKELKK
                     GTVKVGMVYTGDVFLDLCRGGHVKNTLELPIDAFKLTKVAGAYWRGDEKNPQLTRVYG
                     VAFQSKEELDKYLWQQEEAKKRDHRVLGEKLKLFTFAPEVGPGLPLWLPNGTILRDEI
                     ENRAKAVEKEWGYQRVTTPHIAREELYKMSGHLPYYADSMYPAMKLDDGNYYLKAMNC
                     PHTHMIYRAEPKSYRDLPVRYAEYGTVYRYERSGTLAGLLRVRGFTQNDAHIYCTEEQ
                     AEEEFLNVMKLHEYWYKEVFGIADFYMRLSLPSKDKKKYAGAPDGWKKAVVIVRAAMK
                     RSGLPFVEVEDEAAFYGPKIDFQIKSVIGREETASTNQLDFIATQRFGLTYRDASGKE
                     KPVYVIHRAPLGSHERFIAFLIEHYAGAFPFWLAPEQIRIVPISDKFVGYAGKVREIL
                     LEKQKDLRITVDEANETLGKKIREGEIMKIPYLLIAGEKEQTQQNISVRARQKGDLGV
                     MSAQQFVSIIQ"
     gene            2203..3804
                     /locus_tag="VFE94_03255"
     CDS             2203..3804
                     /locus_tag="VFE94_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50136.1"
                     /translation="MTVSPHVTKLLEQYQLWEKESSFPEEEGTISVDEVLSHVASFYE
                     KIRGIVDWREEHLLRKTAIERILKRRMVVHGFSDDFAEGFLSELVRGGHFPNRRISLT
                     KVAEIQSIIEKYVHISTRYRQTNGKSRKNLEDWLYSIAAVEIEESLSNPRRERALIEL
                     MTAALADKIEIASKRKGKVALSPEEQMLQVYVAVQRALFKLDTSTIAYHLLEKFYEDW
                     KNPTEETLTYAAESLGTLHKNIFRILEHPYAEKFYQVAERYDTPYLIVGDIVNEDMDR
                     FPELLDNPAALEERIQQAYNIRLARLKSRIRRAAFYSTLSVFLTKILIALAIEVPLER
                     FVADGTLNYVAMGLNVAFPPLLMLGLVLTTQKTSRRNFERVTLEVMKVIQLHEQQDTY
                     KILPPRKRGGLMAGMVYGFYLLSFLFSFGLLVWVLMQLNFPIFSIIIFLLFVSLILFA
                     GTKIRQRAQELMIESPKENFINNIFDFFSLPLIQTGKWLSGKFVRYNILVLFLNFLIE
                     VPFQMFVEFLEQWRGFLREKKEEIH"
     gene            3801..4688
                     /gene="miaA"
                     /locus_tag="VFE94_03260"
     CDS             3801..4688
                     /gene="miaA"
                     /locus_tag="VFE94_03260"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZX50137.1"
                     /translation="MIVIVGPTASGKSDLGVQLARKFGGEVVSADSRQVYRGLDIGSG
                     KITKKEMMGIPHHLLDVASPRRRFSAAQYQKLAFRAIRDIQRRGKLPILVGGTPFYVY
                     SVVDGFVFPEVEPNPKLRGTLARLSNQELLEKLEELDPERANTLQQSSGQDKNKRRLI
                     RALEIVAATGKPVPKFNKVNPLFDTLMIGVKRSPEELKKRIRKRLLYRIDHGMLNEVK
                     NLHQSGLSWRRLEELGLEYRFVAQYLQKKISKQEMVDSIQRESEDFVRRQMLWFKRDP
                     RIYWIESEKEAEKLVQKFL"
BASE COUNT         1342 a         1041 c         1162 g         1231 t
ORIGIN      
        1 acttcgtagt tttgtaactt gaccaggctc aggttcgtct tctataatag ataatggact
       61 ggagagagta tattccagac ttaacggatg agcagataaa aggtgtcgca gagattttgc
      121 gcgatatggg tcaggtagcc ttggcttcag gagttattcc ctttctgttt ccgatattta
      181 tccccgaacg tgcgtttgca atgcttgttg gattaaccgc agctattatg ttttggtcgg
      241 ctagcattat aatgttaaac tatattaaaa gaccatgagc agtataatgt tattttactt
      301 ggcaatggct atattagcgc ttgctgcggc ggttcttacc catgccgttg taaccacgag
      361 acataaggaa gaccgcaaaa accatccgaa aaatggctaa attttgctag gatccatgga
      421 accctctctt attgaggggg tttttgtttt atggtaaggt atataaattg atatggaaaa
      481 gaatccaatg gagaatatac gccactcgtt tgcgcatgta ctggcctcgg ccgtaaaaaa
      541 gcattatccc aaggcggaat tgggcattgg ccccgttatt gaaaacggct tttattatga
      601 tttcggcaaa gtaaaaatta ccgacgagga tttgccgaaa cttgaagctg aaatgcgaaa
      661 aattgctgca ggcaatcata agtttaaaaa agaactttgg gatcctgcaa aagccaatcg
      721 ttattacaaa aaagaaggcc agccgtttaa actcgattta attaaagaac tcaaaaaggg
      781 tacggtcaaa gtcggcatgg tatacacggg tgatgtattt ctcgacctgt gccgcggcgg
      841 gcatgtgaaa aatactttgg agcttccgat agatgctttt aagcttacca aagtggcggg
      901 agcttattgg cggggggacg aaaaaaatcc ccagttaacc cgcgtatatg gcgtggcatt
      961 ccagagcaaa gaagagctcg acaaatattt gtggcagcaa gaagaagcaa aaaagcgcga
     1021 ccaccgcgtg ctgggcgaaa agctcaagct ctttactttc gcgcccgaag tggggcccgg
     1081 acttccgctt tggttgccca acggaactat tctgcgagac gaaatcgaaa accgcgcaaa
     1141 ggcggtagaa aaagagtggg gctatcaacg cgtaacaact ccgcacattg ccagggaaga
     1201 gctatacaaa atgtctggcc acctccccta ctacgcagac agcatgtatc ctgccatgaa
     1261 actggacgac ggcaattatt atttaaaagc catgaattgt cctcatacgc atatgattta
     1321 cagagcagag cccaagagct accgcgattt gccggttcgt tacgcggaat acgggacagt
     1381 gtaccgttac gagcgttcgg gtacgttagc cggacttttg cgcgtgcgcg gctttaccca
     1441 aaacgatgcc cacatttact gcaccgagga gcaggccgag gaagaatttt tgaacgtaat
     1501 gaaactgcac gaatactggt ataaagaagt gttcggcatt gccgattttt acatgcggct
     1561 ttcgcttcct tccaaagaca aaaagaaata tgccggagct cccgacggct ggaagaaagc
     1621 agtggtaatc gtgagggccg cgatgaaacg ctcggggctg ccttttgtgg aagtagaaga
     1681 cgaagccgcg ttttacggcc ccaaaattga ttttcaaatt aaaagcgtga ttggccgcga
     1741 agagacagcc tccaccaacc aactcgattt tatcgccacg cagagatttg gattgaccta
     1801 tcgggacgca agcggcaagg aaaagccggt ttacgttatc catcgggctc ccctgggctc
     1861 gcacgaacgg tttatcgcgt ttttgattga gcattatgcg ggcgcgtttc ccttttggtt
     1921 agcgccagag caaatccgta ttgtcccgat ttcagataaa ttcgttggct acgccggcaa
     1981 agtaagagaa atattgctcg aaaagcaaaa ggatttgcgt attactgttg acgaggcaaa
     2041 cgaaaccctg ggcaaaaaaa tccgcgaagg cgaaatcatg aaaataccat acctgcttat
     2101 tgcgggcgaa aaagagcaaa cccaacagaa tataagcgta agagcccgcc agaaaggcga
     2161 tttgggcgta atgtcggcgc agcaatttgt ttcaataata caatgactgt ttctccccac
     2221 gttaccaaat tattagaaca gtatcagctt tgggaaaaag aatcttcctt tcccgaggaa
     2281 gaaggcacca tttcagttga tgaggtgctt tcgcacgttg cttcgtttta tgaaaaaatc
     2341 cggggcattg tagactggcg cgaagagcat ttactgcgca aaaccgcaat cgagcgaatt
     2401 ttgaaacggc gcatggtggt gcatgggttt tccgatgact ttgccgaggg attcctttct
     2461 gaattagtgc gcggggggca tttccccaac aggcggattt cattgacaaa ggtggcggaa
     2521 atccaaagca ttattgaaaa atatgtgcat atcagcactc gttaccggca aacgaatggc
     2581 aaatcccgta aaaatcttga agactggctt tattcgatag ctgccgtaga aatcgaggag
     2641 tctctttcca acccgcgccg ggaacgggca ctcatagagt taatgacggc cgcacttgca
     2701 gacaagattg aaattgcctc gaaaagaaaa ggaaaggttg cattgagccc agaagaacaa
     2761 atgctccagg tatacgtagc ggttcagcga gcccttttta aactcgatac ttctaccatt
     2821 gcataccact tgctcgaaaa gttttatgaa gactggaaaa atcccaccga ggaaactctt
     2881 acctacgcag cggaaagcct gggaaccctg cacaaaaata tttttcgcat tttagagcac
     2941 ccgtacgcag aaaagtttta ccaggttgca gaacgttacg acacgccgta tttgattgtc
     3001 ggagacattg taaacgaaga tatggacagg tttccagagc ttctcgacaa ccctgcagcc
     3061 cttgaggagc gaatccagca ggcatacaat atacgccttg ctcggttgaa aagcagaatc
     3121 aggcgagccg ctttttacag cactctttcg gtgttcctca caaaaattct tattgcctta
     3181 gccatagaag ttcccttgga gcgtttcgtg gcggacggca cgcttaatta tgttgctatg
     3241 ggcttgaatg tcgcctttcc cccgttgctc atgctgggtt tggtgcttac tacccaaaaa
     3301 acctcgcgca gaaactttga gcgcgtaacc ctggaagtaa tgaaggttat ccagttgcat
     3361 gaacagcagg atacctacaa aattttgcct cccagaaaac gcggggggtt aatggcaggc
     3421 atggtatatg gattttacct gttgagtttc ttgttctcgt ttggtctgct ggtatgggtg
     3481 cttatgcaat taaatttccc tattttttct ataattattt ttctcctgtt tgtttctttg
     3541 atattgtttg cgggcaccaa gatcaggcag cgggcccagg agcttatgat tgaatctccc
     3601 aaggagaact ttattaataa tatattcgat tttttctctt tgcctttaat ccagacaggg
     3661 aaatggcttt ctgggaaatt cgtgcgttat aatattcttg tgttgttttt gaatttcctc
     3721 atcgaggtgc cttttcaaat gtttgtcgag tttttggaac aatggcgcgg ttttctccga
     3781 gaaaagaaag aagagattca ttgatagtaa ttgttggccc tactgcctct ggaaaaagcg
     3841 atttgggcgt gcagctcgcg cggaaattcg gcggtgaggt tgtttcggca gactcccggc
     3901 aagtatacag gggtctggat attggctctg gcaaaattac caaaaaagaa atgatgggta
     3961 tcccccacca tcttttggat gtggcctctc caagaagaag gttcagtgca gcccaatacc
     4021 aaaagcttgc cttcagggcg attcgcgata tccagcgccg cggcaaactc cccatattgg
     4081 ttggaggcac gccgttttat gtgtattcgg ttgtagatgg ttttgttttt cccgaagtag
     4141 aaccaaaccc taaactccgt ggtaccttag cccggctaag caaccaagaa ttactcgaaa
     4201 aattggagga actggaccca gaacgggcaa atacccttca acaaagttca gggcaggaca
     4261 aaaacaagcg ccgcttaatc cgcgcgctgg aaatcgtagc tgctaccgga aagccagtcc
     4321 cgaagtttaa caaggtcaat cccctttttg atacgttgat gattggagtt aagcgctcgc
     4381 ccgaggaact caaaaagcgt attcgcaaac gcttacttta tcgaatcgac cacggtatgc
     4441 ttaatgaagt aaagaatctc catcagtctg gattgtcatg gagacgttta gaagagcttg
     4501 gattagagta tcgatttgtc gcacaatatt tgcagaaaaa aatctccaaa caggagatgg
     4561 tggattctat ccaaagagag tcagaagact ttgtccgccg tcaaatgctt tggttcaaac
     4621 gcgacccccg catctactgg atcgagagcg aaaaagaggc agaaaaatta gtacaaaaat
     4681 ttctctaggc gacttcgccg agtttgcgct ttacctcaaa cgggttttca atatgggtga
     4741 gtactacttc atggccagac gtgccagcgg tattta
//
LOCUS       DASRUP010000080         6807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_926879, whole genome shotgun sequence.
ACCESSION   DASRUP010000080 DASRUP010000000
VERSION     DASRUP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926879"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /gene="atpD"
                     /locus_tag="VFE94_03265"
     CDS             <1..1088
                     /gene="atpD"
                     /locus_tag="VFE94_03265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZX50138.1"
                     /translation="GNPIDGKGEVKAERSSIYQPAPPLDQQKTEPEVFETGIKVIDLL
                     APMLKGGKVGLFGGAGVGKTVLLQELIANTAREHGGYSVFAGVGERTREGNDLYREME
                     ESGVLKNTAMVFGQMNEVPGARFRTALAGLRMAEYFRDEKNKDVLLFIDNIFRFVQAG
                     SEVSVLLGRMPSAVGYQPTLATDMGQLQERITSTKKGSVTSVQAIYVPADDITDPAPA
                     TTFSHLDSSIVLSRELASLGIYPAVDPLASRSSALDPRIVGEEHYQVARQVQQILQRY
                     KELQDIIAILGMEELSDEDKQTVNRARRIQRFLSQPFFVAAQFTGREGKYVPLADTVR
                     GFKEIIEGKHDAKEESDFYLKGGIEEV"
     gene            1370..1642
                     /locus_tag="VFE94_03270"
     CDS             1370..1642
                     /locus_tag="VFE94_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50139.1"
                     /translation="MVILHFLQKYKVGAIGGLIYGTLGFMVLYGLGACRTSQGACVAF
                     SPLVFHQTIVQLVAANLATPLFLIPLNAFVFIFEGAAIEMLTRKLK"
     gene            1746..1991
                     /locus_tag="VFE94_03275"
     CDS             1746..1991
                     /locus_tag="VFE94_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50140.1"
                     /translation="MNFPLRVFAIDREIFVGDAKSLIVPTTTGQIQILAEHTPLVSIL
                     KEGEVVIESADGTMQKLPILGGTVEVTDTEVVCLVNF"
     gene            1993..2565
                     /locus_tag="VFE94_03280"
     CDS             1993..2565
                     /locus_tag="VFE94_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50141.1"
                     /translation="MSNLQKIVILCVVAGIVVFGGFFLLNNYIYQEKQAEDAEQVVEQ
                     AEVMEEAQDEERSQLFSPEEVNAYVGTYGYGNVGEIGGSGILTIRETRPGILFFQLDV
                     NRGPPSYNMGYIEGEIALNEGIATYTTNEYGVCALAFQFEEDKLVVSYINEQDDCGFG
                     GNVHANREYLKTSPEPNDISPWYWKNEGGE"
     gene            3998..4405
                     /locus_tag="VFE94_03285"
     CDS             3998..4405
                     /locus_tag="VFE94_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50142.1"
                     /translation="MNKIWKIVILCGVVVAIAGGAGFYFFAQNGTEKEIEQTPKNLND
                     LARDARIKNEVNTLRAALEIYFVQNAQIGYPTSLSDLGPDNPWPNVAEPERFSYQQTN
                     NGQGYTLEASLHSGETVRYEESMEPSSESPETF"
     gene            4841..5221
                     /locus_tag="VFE94_03290"
     CDS             4841..5221
                     /locus_tag="VFE94_03290"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZX50143.1"
                     /translation="MINQHTCQALAIHCIDFRFQKVLTDYLESRFAGSYDRVAVAGGV
                     KNLPLEQCEVSVRLHSPKTIVLIQHEDCGAYGGSKQFADLEAEKAFQTQEIQKAAKVL
                     QEKFPGCAVEGYVMGLLGDLTSVL"
     gene            5231..5470
                     /locus_tag="VFE94_03295"
     CDS             5231..5470
                     /locus_tag="VFE94_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZX50144.1"
                     /translation="MPSVLMYTTPTCVYCREAKEFFKEHNVEYEEKDVVQDEQARNDM
                     IQKSGQMGVPVIDVNGEIVIGYNKKKLVELLGIQA"
     gene            5475..5696
                     /locus_tag="VFE94_03300"
     CDS             5475..5696
                     /locus_tag="VFE94_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50145.1"
                     /translation="MDIIGALELVGFTLDVAGKIMIAFTAIMVHQRFWKEHKVDEKVF
                     QTMRRERLVGIWGIVFIITGFFLQLPSKF"
     gene            5697..6518
                     /locus_tag="VFE94_03305"
     CDS             5697..6518
                     /locus_tag="VFE94_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZX50146.1"
                     /translation="MEPISFAFIVSAFVAGVITFLAPCTLPLVPGYLSFISGASVADL
                     QNPEKAGKAKAKIFLNGLFYVIGFSAVFILFGSLFGLGGSLLFQYRDILIQIGGAFVI
                     LFGLFLAAPAITGVTNGRINLNRYPPFSFLSAERQFHIGKKLTPGNPISSLLFGGTFA
                     LGWSPCVGPILGVILTLAASSATVSQGAILLFVFSFGLAIPFLLTALAVGWASTHFSQ
                     ITKYLTWVSLLGGIFLIVLGYMMVTGDFIVLNSLIFRFLAGIGLGRYEESLLDLL"
     gene            6535..>6807
                     /locus_tag="VFE94_03310"
     CDS             6535..>6807
                     /locus_tag="VFE94_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZX50147.1"
                     /translation="MQENGNSQNLLPTVFVIFGATGDLMRRKLTPGLFHLYREGHLPK
                     LFQVVGFAKDGMDSESFRAMVEGIVRERVPDAPEEQIASFTQLFVYQ"
BASE COUNT         1784 a         1396 c         1819 g         1808 t
ORIGIN      
        1 tgggcaaccc catagacggc aaaggcgaag taaaagcaga gcgctcgtct atttaccagc
       61 cggcgccgcc tttagaccag caaaaaacag agcccgaagt ttttgaaacc ggcattaaag
      121 ttatagactt gcttgccccc atgctaaaag gaggaaaagt tgggttgttc gggggagctg
      181 gcgtcggaaa aaccgtgttg ctccaagagc ttatcgccaa cacggcgcgc gagcacgggg
      241 ggtattcggt gtttgcggga gttggcgagc gtacccgcga gggaaacgac ctgtaccgcg
      301 aaatggagga atcgggcgtg ttaaaaaaca cggccatggt atttggccaa atgaacgaag
      361 tgccgggcgc gcgttttcgc accgccctgg ctggccttcg catggcagaa tattttcgcg
      421 acgagaaaaa caaagacgtg ctattattta tagacaacat tttccgcttt gtgcaggcgg
      481 gttctgaagt gtcagtgctg cttggccgca tgccttctgc cgtggggtac cagcccactt
      541 tagctaccga catgggccag ctgcaagaac gcattacttc taccaaaaaa ggatcggtaa
      601 cttcggtgca ggcaatttac gtgcctgccg acgacattac agaccctgct cccgcaacca
      661 cgttttcgca tttggactct tctattgtgc tttcgcgcga attggcttcg ttgggcatat
      721 atcctgccgt tgacccgttg gcttccaggt ccagcgcgtt agacccccgc attgtgggag
      781 aggagcacta ccaggtagcc cgacaggtgc agcaaatttt gcagcgctac aaagagctgc
      841 aggacattat tgccattctt ggcatggaag agctttccga cgaagacaaa caaaccgtaa
      901 accgggcccg ccgcatacag cggttcttgt cacagccatt ctttgtggcg gcgcagttta
      961 ccggccgcga aggaaagtat gtaccgctag ccgacactgt ccgcggattc aaggaaatta
     1021 tcgaaggcaa gcacgacgca aaagaagaat ccgacttcta tctaaaaggc ggcattgaag
     1081 aagtgtagtt ttaaaaaaag aagggccgca gaaatctgca gcctttcgag aatcctagga
     1141 tttggtagga tttagccggg gggcggccat ggatggtaaa aacctaagcc gagtacgcct
     1201 actatagcca aaacgattcc tccgtacgct aggttcattg catatcgagt attagtttta
     1261 gcatgtatag tcagccccgc tataagtgca attatcccta taattaatag gagcaaaaaa
     1321 gctaattcta tcatggtcca tcctcctgaa taagcttgaa tcccgtattg tggttatact
     1381 acacttttta caaaaataca aagtaggggc aataggcggg ttgatatacg gaacacttgg
     1441 gtttatggtg ttgtatggcc ttggagcttg ccgcacttcg caaggggcat gcgtggcgtt
     1501 tagcccgctg gtgttccacc aaactatagt gcagttggta gcagcaaacc ttgccacgcc
     1561 gttattccta atcccgctaa atgcatttgt atttatcttt gaaggcgccg caattgaaat
     1621 gctaactcgc aaattaaaat gaaaaatcgc aaacttttga aaattctcat tattgcggta
     1681 atcagtgtag ttatactttg gtttttgtat ggttggatat caactgcatt atgggaaaga
     1741 gtaatatgaa ttttccactt agagttttcg caatagacag agaaatattt gtgggcgacg
     1801 caaaatctct gattgtccca actacaacgg gccaaattca gattttggca gagcacacgc
     1861 cgttagtaag catccttaaa gagggcgaag ttgttattga atctgccgac ggcaccatgc
     1921 aaaaactccc tatcctcggc ggcaccgtcg aagtaactga taccgaggtt gtttgtttgg
     1981 ttaattttta atatgagcaa tctacaaaaa attgtaatat tgtgtgtggt ggcgggcatt
     2041 gtcgttttcg gaggcttctt cctgctgaat aactatattt atcaagagaa gcaggcggag
     2101 gatgcggaac aggtagtgga acaagcggaa gtgatggagg aagctcaaga tgaagaaaga
     2161 tcacaacttt tttcgccaga agaggttaat gcgtatgttg gaacgtatgg ttatggaaat
     2221 gtcggggaga taggtggcag tggtatcttg actattagag aaactcggcc gggcatactc
     2281 tttttccaac tagacgtaaa tagaggccca ccatcgtaca atatgggcta tatcgaggga
     2341 gaaattgctt tgaatgaggg cattgcaaca tacacaacca acgaatacgg tgtatgtgcg
     2401 cttgcctttc aattcgagga ggacaagtta gttgtttcgt atatcaatga gcaagatgat
     2461 tgtgggtttg ggggtaacgt acatgcaaat agggagtatc tcaaaacaag cccggagcct
     2521 aacgatatta gtccgtggta ttggaaaaat gaaggtggag aatgaaaaat accgaaaggc
     2581 gaggggtggg tattcgaggt ttttggacgg ggcgtgcgca aagtgcatgg gctaggactg
     2641 catgtgctag gaccgtccta gcacagcaaa cgcttttttg cagccgcggt ttccaaaaaa
     2701 gtcataaact ccgaatcgct gggttgattt ttcctaagag ataagtataa tgacgctcaa
     2761 gaaaggaggt gatcgttttg acgtctatta cagttctatt gtacattgct ctgggggcgg
     2821 ggattggggt tgttattgag ttttttgtgc gtcgccgcta ttactggagc tttccctgac
     2881 caattatcat gggcgcggtc ggcgccataa ttgggctggc agcatcagca ttcttgtatc
     2941 ttcatgtact tcccctcgta cttatggggg tgctgctcgc tattatcctt gggcttgcac
     3001 ttttgcctcg gcgtagacgg aatgtgtaag cccctcccac tttggctggg acgacatgtt
     3061 cgttcccggc ctttctattt gcataatgta gccggcaaga agccatttga actgcttgaa
     3121 ccgtttggac aacttgaacc gctttgccca atcaaaccac tcacaaaaca atcctacaga
     3181 ccagtccata ggactaaaag gctaagcgag tgggctaaac caggtttagc ccagccttag
     3241 cgtcattgaa acctccggag agggcagatc gcattttcgg cggttttttg gtagagtatg
     3301 gatagaaaga tcattggagg tgtcgcttga agaaaggcag tagtagggtt atacgtcccg
     3361 atatcaggca cctgcaggaa atttgtagaa gaaattgggg gttgcagatt agagaaatgg
     3421 caaagccgtc ggatgcgcgg ccaggcctgc cgactggcaa agttatccat agccgttagg
     3481 gaaaaaggag cgtgcgacgc agaagcgtct aaaaatggaa agtacccaag gcctccggca
     3541 aaattcgctg gaggcctttc tattttcata cggtgccggc ggagaaggca gtgttgcttt
     3601 ttgtttttct tatttgtata atcgctaaca tcaccgccaa ggaggggttg aaatgagtgc
     3661 cggttgtatg gaaccaggtg gccactcttt cgatactctc gagcttatcg agggcgtttc
     3721 ggaggatggc cgaaatgcaa gctttgaagt tcttgcatgt aggaattata ggtgcgacgc
     3781 cgttgtgcca gatcccgtag caaactttat gcggtggtca gaggaagaac gccgacagtt
     3841 tatgagggta atggccgagt ggggaaggac tgtagtattc cccgatcctc tataagcccc
     3901 gtttttgttg ctatgccaga caacatgcgg ggcgttttgt ttgcttttct ataaaaaact
     3961 gacgaggcaa gtaaaagtgt ggtagaataa atatagtatg aataaaatat ggaaaattgt
     4021 catattgtgc ggggtagtgg ttgctattgc cggaggggcg gggttttact tttttgcgca
     4081 aaatggaaca gaaaaggaaa ttgagcaaac tcccaaaaac ctgaatgatt tggccagaga
     4141 cgcgcggatt aaaaacgaag taaacactct gcgcgcggcc ttggagattt attttgtgca
     4201 aaacgcgcaa ataggatatc ccacctcgtt atccgatttg gggccggata acccgtggcc
     4261 caacgtggca gaacccgagc gtttttcgta ccagcaaaca aataacggcc agggctatac
     4321 gttagaggct agtttgcata gtggcgaaac agtgcgatac gaagaatcta tggagccgag
     4381 ttcggagtcc ccagaaactt tttagcgcag ctaggccttc ccgttgcttt tatggttacg
     4441 gtagggtata aaaagagagt ccaggatttc tctgtctagg aggtaatcat ggtctgccag
     4501 tatccgcacc ttacatggag agccgccgcg cctgggcgtt gctacaagcc tggggatatc
     4561 ggcatatttg agggaaagcc tcaagaagta gttgggagtt ttgggcaatt ccatagctac
     4621 gacaacgagg ctccggttgt cttctctgaa ggagacactt caggattctg cgacggcgta
     4681 gaaacacttg ggtggacgtg taaaaccctg gaatactgta tccacgaccc agtttgtgta
     4741 gtgtacatca aataccatga aatcgaacgc gatccttcta accctcccga cacaagttag
     4801 gaggttttat ttttagacta aaatctggta agcttatact atgataaacc aacatacatg
     4861 ccaggcgttg gcaattcatt gcatagattt tcgttttcaa aaggttttga ccgattacct
     4921 ggaaagtagg tttgcgggta gctacgaccg cgtggccgtg gccggaggag taaaaaacct
     4981 gcctttagag caatgcgagg tgtcggtaag actgcatagc ccaaagacca ttgtgcttat
     5041 tcagcatgaa gactgtgggg catacggggg cagcaagcaa tttgctgatt tagaagccga
     5101 gaaagcgttt caaacgcagg aaatacaaaa agcagcaaag gttttgcaag aaaagtttcc
     5161 aggttgcgcg gtagaaggat atgttatggg gcttttggga gatttgactt ctgtgttata
     5221 atagaaatat atgccaagtg tattaatgta tacaacacca acatgcgtgt attgccgcga
     5281 ggcaaaggag ttttttaagg aacataacgt ggaatacgaa gaaaaggatg tggtgcagga
     5341 tgaacaggcc agaaacgaca tgatccaaaa atccggccaa atgggcgtgc ccgtcatcga
     5401 cgtgaacggg gaaattgtta ttggatacaa taagaaaaaa cttgtggagt tgcttggaat
     5461 acaggcgtaa atgtatggat attatcggcg cgttagaact tgttggcttt acccttgatg
     5521 tagcggggaa aattatgatt gcctttaccg cgattatggt acaccaacgg ttttggaaag
     5581 aacataaagt agacgagaaa gtgttccaaa caatgcgccg cgagcgtttg gttggaattt
     5641 ggggaattgt atttattatt acgggtttct ttttgcagct accctcaaag ttttaaatgg
     5701 agccaataag tttcgctttt attgtatccg cgtttgtggc gggggttatt acgtttttgg
     5761 cgccttgcac tttgccgttg gtgcccggat atttgagttt tattagcggg gcttcagtag
     5821 cagacctgca gaatccggaa aaggcgggca aggcaaaagc aaaaatattt ttaaacggac
     5881 ttttttatgt aatcggcttt agcgccgtat ttattttatt cggaagttta tttgggttag
     5941 gtggttctct gctgtttcaa taccgtgata tacttatcca aataggcggg gcgtttgtca
     6001 ttttgttcgg attgtttttg gcggcgccgg ccataaccgg agtaacgaac ggcagaataa
     6061 acctaaacag atatcctccg tttagttttt tgtccgcaga gcggcagttt catattggca
     6121 aaaaactgac tccgggaaat ccgataagct ctttgctttt tggcggaaca tttgctttag
     6181 gctggagccc gtgcgtgggc cccattttgg gagtaattct tacgctggcc gcgtcttccg
     6241 ctacggtaag ccagggggca attctgctgt ttgtgttttc tttcgggttg gcaatcccgt
     6301 ttttgcttac cgcgcttgcc gtgggttggg catctacgca cttttctcag attaccaagt
     6361 atttaacctg ggtttcttta ctgggcggca tatttttaat agtgctgggg tatatgatgg
     6421 taacgggcga ctttatcgtt ttgaacagcc ttatttttcg ttttctagcg ggtatagggc
     6481 tgggtagata cgaagaatcc ttgcttgatt tgctctaaga ggtacaatag cacaatgcaa
     6541 gaaaacggaa attcgcagaa tttgctgccc acggtatttg tgatctttgg tgccacggga
     6601 gaccttatgc gccggaagct aactcccggg ctttttcatt tgtaccggga agggcatttg
     6661 cccaagctct tccaggtggt ggggtttgcg aaagacggca tggattcaga atcgttccgt
     6721 gcgatggttg aaggcattgt acgagaaaga gtgccagacg ccccagagga gcagatcgcc
     6781 tctttcacac agctttttgt atatcag
//
LOCUS       DASRUP010000081         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1555146, whole genome shotgun sequence.
ACCESSION   DASRUP010000081 DASRUP010000000
VERSION     DASRUP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555146"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..232
                     /locus_tag="VFE94_03315"
                     /pseudo
     CDS             107..232
                     /locus_tag="VFE94_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            248..1069
                     /locus_tag="VFE94_03320"
     CDS             248..1069
                     /locus_tag="VFE94_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50148.1"
                     /translation="MTFRFSNSFLQDSWNANAIESIRIELLEKDGVGARGGFYEGIGA
                     LRDVGQNHLLQLLALLCMDNPGGFGAELVRKKRAELLESLEIFGKGEVAKKTVRGQYE
                     GYRREKDVDPNSQTETYFRIEASLNTDKWRGVPIYLEGGKAMKEEKVEATITFRHPTP
                     CLCPPEQGKHYKNVLYYRIQPKEGIHIVFWVKKPGPKMVVEEKEFAFDYKAAFGGEEF
                     LDAYVRLLHNVIQGDQTLFVSTEEIMAEWKFVDPILRAWQKGITPLTIYQKNTMP"
     gene            1066..1938
                     /gene="gnd"
                     /locus_tag="VFE94_03325"
     CDS             1066..1938
                     /gene="gnd"
                     /locus_tag="VFE94_03325"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HZX50149.1"
                     /translation="MKKHIGYIGLGKMGASMVARLKETGWWVESYDISGKGTAKSIEQ
                     LVGRLQKPRVVWIMVPAGKPVDDVISQLAEMLDKRDTVIDGGNTFYEDSVRHGKELAA
                     KGIAFLDVGVSGGPGTVRQGKPAVMVGGDKKVFQKLKPLFGDLTAKESFGYMGTSGTG
                     HFVKMAHNGIEYGMMQALAEGFALMQKSPFELDLKEVARVYNNGSVIESRLTGWLASG
                     LKKYGEALTEVSGKVAHTGEGEWTVKTAKKLGVPAPVIRTSFDFRVQSAKKPSYTGKV
                     LTMLRNQFGGHNIK"
     gene            1940..2764
                     /locus_tag="VFE94_03330"
     CDS             1940..2764
                     /locus_tag="VFE94_03330"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZX50150.1"
                     /translation="MNIHRLHTREEARASAISEFETLVKEAQGSPLLLLFSGGSALEL
                     LGGFQEDLLQGYDSTIGVLDERYSKDPGTSNFAQLATSRFYQEAKMRGVHSIDTRAYE
                     GLHFQDIGERFAQALKEWKRKNASGKVIITQGIGEDGHTAGIMPYPLHRERFGKLFEN
                     ADKWVVAYDVRETEEKEVQPAPATRQNPLFARTVRGTTMLWQGRPKHTEEAFPMRITV
                     TLPFLRNIVDHSIVYAVGARKERILSRVLLEHGELWEFPARVIHEMKDVRVFCDIA"
     gene            2761..>2903
                     /locus_tag="VFE94_03335"
     CDS             2761..>2903
                     /locus_tag="VFE94_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZX50151.1"
                     /translation="MKPENLKTKIFLDSGDPAETKEAIELLGFLDGQTTNPSLIAKNP
                     ETTG"
BASE COUNT          821 a          621 c          830 g          631 t
ORIGIN      
        1 gaacaagctg ttttatattg cagcttcccc gcagttttac aaaacgctgt ttctccagct
       61 ggcaaagtcg cggctcacgt aaccctgcag ccttaaagaa ggctggacgc gcgttatcgt
      121 ggaaaagccc ttcgggaaag acctccaaac cgcccaggaa ctcgataaac tgttgggcaa
      181 gctttttaaa gaagagcaga tttatcgaat cgaccattat ttgggcaagg aaaccgtaca
      241 aaacattttg acctttcggt tttcaaattc ttttttgcag gattcctgga acgcaaatgc
      301 catagaaagc attcggatcg agctgcttga aaaagatggg gttggggcgc gcggaggatt
      361 ttacgaaggc attggcgcct tgcgcgacgt ggggcagaac catttgctgc agcttttggc
      421 gctgctgtgc atggacaatc cgggcggatt tggcgcagaa ctggtgcgca agaaacgggc
      481 agagcttttg gagtcgctcg agatttttgg caaaggcgaa gttgccaaaa aaacagtccg
      541 cggccaatac gagggctatc gcagagaaaa agacgttgac ccaaactccc aaaccgaaac
      601 atattttcgc atagaggcgt ctttgaatac agacaaatgg aggggtgtgc ctatatattt
      661 ggaaggagga aaggcaatga aagaagagaa agttgaggca acaataacgt tcaggcaccc
      721 taccccttgc ttatgcccgc ccgaacaggg caagcactat aaaaatgttt tgtactaccg
      781 tattcagccc aaagaaggca ttcatattgt gttttgggtg aaaaagccag ggcccaaaat
      841 ggtcgtggag gaaaaagagt ttgcctttga ctataaggcg gcattcgggg gcgaggagtt
      901 tttggacgct tatgtgcggc ttttgcacaa tgttattcag ggggaccaaa cgctttttgt
      961 gagcaccgag gaaattatgg cagagtggaa gtttgtcgac cctattttgc gggcgtggca
     1021 gaagggtata acaccactta caatttatca aaagaatact atgccatgaa aaaacacata
     1081 gggtacattg gtttgggaaa aatgggggcc agtatggtag ccaggctcaa agaaacaggc
     1141 tggtgggtgg agtcgtatga tatttcgggc aaaggcacgg caaaaagcat tgagcagtta
     1201 gttggaaggt tacaaaagcc ccgcgttgtt tggattatgg tgcctgcagg caagcccgtg
     1261 gacgacgtaa tttcccagct tgcagagatg ctggacaaaa gggacacggt aatcgacggg
     1321 ggaaacacgt tttacgagga ttcagtgcgg catggcaaag agctggcagc caagggaatt
     1381 gcgtttttgg atgtgggggt ttcgggcggc cctggcacgg tgcggcaagg caaacccgcg
     1441 gttatggtgg ggggcgacaa aaaagtgttt caaaaactca aacccctgtt tggcgacctt
     1501 acagccaaag aaagttttgg gtatatggga acatcgggaa ccggccactt tgtaaaaatg
     1561 gcccataacg gtattgaata cggcatgatg caggcgttgg ccgaagggtt tgcgcttatg
     1621 caaaagtcgc cgtttgaact cgatttaaaa gaagtggcca gggtctataa taacggaagc
     1681 gtaatcgaat cgcggctgac cgggtggctg gcaagcgggc ttaagaaata cggggaagcg
     1741 ttaacagaag tatcgggcaa agtggcgcat acaggcgagg gggaatggac cgtaaaaacc
     1801 gcaaaaaagc tgggcgtgcc cgcgcccgtt ataagaacat cctttgattt tcgcgtgcag
     1861 tcggctaaaa agccaagcta caccggaaaa gtcttaacaa tgctgcgcaa ccaatttggc
     1921 gggcacaaca taaagtagca tgaatattca ccgcttacac acaagagaag aggcgcgggc
     1981 cagcgccata agcgaatttg aaacattggt gaaagaggcg cagggcagcc cgctgctcct
     2041 gctgttttcc ggcgggtctg cccttgagct gttgggaggc tttcaggagg atttgctgca
     2101 agggtacgat tcaaccatag gtgtgctcga cgagcgctac agcaaagacc cggggacaag
     2161 caattttgcg cagcttgcca ccagcaggtt ttaccaggag gcaaaaatgc gaggcgtaca
     2221 ctcgatcgac acgcgcgcgt acgagggttt acactttcag gatatcggcg agcgatttgc
     2281 gcaggcgctc aaagaatgga aacgaaagaa tgcttcaggc aaagttatta ttacccaagg
     2341 aataggggaa gacggccata ccgcaggaat tatgccttat ccgttgcaca gggagcgttt
     2401 tggcaagctt tttgaaaatg ccgacaaatg ggtggttgca tacgacgttc gggaaaccga
     2461 agagaaggaa gtgcagcctg cgccagctac aaggcaaaat ccgcttttcg cgcgaacagt
     2521 acggggtact accatgcttt ggcaaggaag gcccaagcac accgaggaag ctttccctat
     2581 gcgaattacg gtaactttgc cctttttgcg caacatagtg gaccattcaa tagtctatgc
     2641 ggttggcgcc agaaaagagc gaattctctc tcgcgtgctc ttagagcacg gggagttatg
     2701 ggaatttccc gcgcgcgtta ttcacgaaat gaaagatgtg cgcgtgtttt gcgatattgc
     2761 atgaaaccag aaaatttgaa aacgaaaata tttttggata gcggagatcc tgcagaaacg
     2821 aaagaagcaa tagagctgct cggatttttg gacggccaaa ctacaaaccc gtcgcttatc
     2881 gcaaagaacc cggaaaccac agg
//
LOCUS       DASRUP010000082         5651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1609474, whole genome shotgun sequence.
ACCESSION   DASRUP010000082 DASRUP010000000
VERSION     DASRUP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609474"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3406)
                     /locus_tag="VFE94_03340"
     CDS             complement(<1..3406)
                     /locus_tag="VFE94_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50152.1"
                     /translation="MSTIAKKIFVGAVAAVMVATVAMPPVAQAQTIEQLQAQISSLLA
                     TIAQLQTQLATLAGGSTGGGLSGIPAGFTFTGNLSQGMSGQDVMYLQILLNSSADTQL
                     ASSGAGSPGSETQYFGPITRTGVVKFQNKYASEILTPLGLTAGTGFFGAGSRAKANML
                     LAAAPAPTPTPTPTPTPGTPPPTPPVVTPPAGNAVQVTLASDTPASTNIADAGNGNFT
                     KFWFTAGSSDVSVSKVVVTRKGLTANADLENIKLVDMNGVSRGSVGSLNVNNKSTLTF
                     SPALVIPANSSKGYYIRAGFVDGTTAGKTANFGIEANSDVTAGSATVMGAPIYGNEMS
                     VVSLTIGSVEAKEDGTTPDSQPDVGDIDVPVNNFKVVVGSTEAVTIEQITVQESGSAS
                     LTDTGNIELWDVTGNKSLGTVASWDAEGRASWANLNVVVDKGKTHRFKVRLDILDGPS
                     LTVNADLMDGSDVLMAVKGNTYNFYITPDGNDTDDWTNSNDGLGSSNQTIAAGSMVVS
                     KSPSTPATGNIAAADDQKLATFDYVVKGEEMRVSSTVIDFDFSGLTCAETTSVRIVDK
                     DNTIVAGPSDCASDALTFSDTYIVPVGTNMYSIIANISSNASTGDTVDVGIDQPGDMT
                     VRGVTSNNTVNATPATTEVEGNTLTVKAGDLDATTLATPAARSISQGIQDFIFMTGSL
                     SASDSGEDVNVTAITIANDPSSGASADEVINVEIWADMTAANSARGDVYETKIGDSVD
                     YDETVNDTADTIAFTLNQTVTVMKGSFVNIAVVADLSTSATDTETHTVDISAVTANGA
                     STGTSITVTPTGSGQAMTVTTGGTLTTTVDSSEPAASLFAANSTETTIAVFRLAANNV
                     EDLDLDDIDLRVSGGFKVATYKFYDGTTLVKTATGGVGSSTTAQSLNVPFDDNSFVVP
                     AAGNKKLTVKVDFLGTTTLAADNNLDVEVTMLNADSTGKSSGTTISGATDLTANAHSY
                     FLSYPSVTVSSSSPASGNLIPSANTLLAVFDVKAGAAEDITFSTGDANNFTVSLSVTI
                     GDSTDAAESFELKDENGISLDTVSTDLSVVGGDIDLAFDFTDAVFTVPAGQTKKLYVY
                     GDTGDLEDDGDSIQAWLDDGTATFIDWSIDSTGSYNHADRIFRGDLF"
     gene            complement(3507..4298)
                     /locus_tag="VFE94_03345"
     CDS             complement(3507..4298)
                     /locus_tag="VFE94_03345"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HZX50153.1"
                     /translation="MEEKELLSKISLLKSVKPREEWVFSTRQELLGDRVPKKSFFNLQ
                     KERFSGMLWHTAFLFKFAFSKPVLVVPVLVFLAVGGALLWQVSLRSLPGDALYSLRTT
                     VEQVPLSFSAEEEKVFLQIELAQKHLADLRRMAEENKIRNLASAIKIYEANATQVSQS
                     VTLIVENQPDKALQAGSQIVQLQKEKAEIEKILGAKIGEEHAESLENATKKIVEYEFA
                     DLETRSLTEGQLALFESAKADVEQGNYELALEKLLLISVDIHGEG"
     gene            complement(4346..4861)
                     /locus_tag="VFE94_03350"
     CDS             complement(4346..4861)
                     /locus_tag="VFE94_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX50154.1"
                     /translation="MEERFGEIYDQYIIKIYRFIVLKVDSQEVAEDLCSEVFTRVFQE
                     FQKQEIQNMQAFLYQVARHIIADYYRDRASFRIVRIEEAEGLVAREHSLLEKAATLSE
                     IEEVKQALANLRDEYQNLIIWRYLDELSIPEIAQLEGKSEGSVRVSLHRAMEALRGKL
                     EVPKDAPSVPV"
     gene            complement(4944..>5651)
                     /locus_tag="VFE94_03355"
     CDS             complement(4944..>5651)
                     /locus_tag="VFE94_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HZX50155.1"
                     /translation="LSRVSLTHPLASDILWKSDLQVMNRNFFVRLAFAAHRVADALPA
                     DEKVTEEIRETANAILVDLLLLTDKEFVSGEQKKELLPRLERELDVLLIYLDTAEKGG
                     WVKPENFALLKTEYTRIRDFLEIFEDFQETKAAVEPKKEPMHSVATAKAPVSQASRER
                     VVRQTPGKQEGADLTERQQRIIDFLRTKHQAQVWELQKVLPQVTKRTLRRDLDELLQK
                     NLVERKGEWNAVAYELK"
BASE COUNT         1287 a         1499 c         1301 g         1564 t
ORIGIN      
        1 cgaagagatc gccacggaag atcctgtctg cgtggttgta actccccgtg ctgtcgatag
       61 accagtcaat aaacgtggct gtgccatcgt ccagccaggc ctgaatcgaa tctccgtcgt
      121 cttccagatc gcctgtgtcg ccatagacat acaacttctt ggtctgccct gccggaacag
      181 taaagacagc gtccgtgaag tcaaatgcca ggtctatgtc gcctcccact acagacaagt
      241 ctgtcgaaac agtgtcaagc gagattccgt tctcatcttt caactcgaag ctttcagcag
      301 cgtcagtaga gtccccgatt gtcaccgata agctcaccgt gaagttgttg gcgtcgccag
      361 tcgagaaggt aatatcttcc gcggcgcctg ctttcacgtc gaatactgcc aacagcgtgt
      421 tcgcgctcgg aatcaagtta cccgatgcgg gtgaggaaga actcaccgta acgcttgggt
      481 aagacaggaa gtagctgtga gcgtttgccg tcaggtcagt cgcaccggat atagtggtac
      541 cggaagactt gcctgtgctg tctgcgttga gcatcgttac ctccacgtca aggttgttgt
      601 ctgccgccaa agtcgtcgtg cccaggaagt caacttttac ggtaagcttc ttgttaccgg
      661 ctgccgggac aacaaacgaa ttgtcgtcaa acggaacatt cagactttgg gcggtagtgg
      721 aacttcctac tccaccggtt gctgtcttca caagcgtggt cccgtcgtag aacttatacg
      781 ttgccacctt aaagcctcct gatacccgca agtcaatgtc gtccaagtca aggtcttcca
      841 cattgtttgc ggcaagcctg aagactgcta tggtcgtttc tgtgctgttg gccgcaaaaa
      901 ggctcgcagc cggttcagac gaatcaacag tcgttgtcag ggtacctccg gtggttacgg
      961 tcattgcctg gccgcttccg gttggggtta ccgttatgct ggtacccgta gaagctccat
     1021 tggcagttac cgcagaaata tccaccgtat gggtttccgt gtcggtagcc gaagtcgaaa
     1081 ggtcggccac aaccgcgatg ttcacgaatg aacctttcat taccgtaaca gtctggttca
     1141 gcgtaaaggc aatcgtgtct gcggtgtcgt tgactgtctc gtcgtagtca accgagtcgc
     1201 cgattttggt ttcgtacaca tctcctctcg cgctgttagc ggcagtcata tcagcccaaa
     1261 tctccacatt aatcacttcg tccgccgaag ctccggaaga tggatcgttg gcaatggtta
     1321 ttgccgttac gttcacgtct tctcccgaat cagaggcaga caagcttcct gtcatgaata
     1381 tgaagtcctg aatgccctgc gaaatgcttc gggccgcggg tgtcgcaaga gtcgtcgcgt
     1441 ccaaatcacc ggcttttacc gttaaggtgt tgccctcaac ttcggtagta gcgggtgtag
     1501 cgttaaccgt attgttgctg gtaactccgc gcacggtcat gtctccaggc tggtcaattc
     1561 ccacgtcaac ggtgtctccc gttgaagcgt tgctggaaat gtttgcgatt atcgaataca
     1621 tattcgttcc gacaggaacg atatacgtat cgctaaacgt cagcgcgtct gaagcacagt
     1681 cgcttggtcc tgcaacaata gtgttgtctt tgtcaacaat tcttacgctt gttgtttccg
     1741 cgcatgtcaa gccgctgaaa tcaaaatcaa tcaccgtcga agaaactctc atttcttctc
     1801 ccttcacaac gtagtcaaag gtggcgagtt tttgatcgtc ggcagccgca atgttgccgg
     1861 tcgcaggcgt agatggagac ttgctcacca ccatgctgcc cgcggcaatc gtctggttgg
     1921 aagaacccaa cccgtcgttg ctgttggtcc agtcgtcggt atcgtttcca tctggagtaa
     1981 tgtagaagtt atatgtgttg ccttttacag ccattaacac atcgcttcca tccattaagt
     2041 cggcgttcac ggtcaggctt gggccatcaa gaatatccaa gcgcacctta aagcgatggg
     2101 tctttccttt atccaccacc acgttcaagt ttgcccagct tgcccttcct tctgcgtccc
     2161 aggaagcaac tgtacccaac gacttgttgc ccgtaacatc ccacagttca atgttgcccg
     2221 tgtctgtcaa agaggcggat ccggattcct ggacagtaat ctgttcaatc gttacggctt
     2281 ctgtggaacc aaccaccacc ttgaagttgt tcaccggaac atcaatatct cccacgtctg
     2341 gctgggaatc aggcgttgtg ccgtcttctt ttgcttctac agaaccaatc gtaaggctca
     2401 ccaccgacat ttcgttaccg tatatcgggg cgcccattac ggttgccgat cccgcggtaa
     2461 cgtcgctgtt ggcttcaatg ccaaagtttg ctgtttttcc tgctgtcgtg ccgtcaacga
     2521 atccggcccg aatgtagtat cccttgctcg aatttgcagg gatcaccaaa gcagggctga
     2581 atgtcagggt tgacttgttg tttacgttca agcttcccac gctgcctctc gatactccgt
     2641 tcatgtcaac aagcttgata ttttccaagt ccgcgttagc ggtaaggcct tttctggtta
     2701 ccacaacttt ggataccgaa acatcggaac ttcccgcggt aaaccagaac ttggtaaagt
     2761 tgccgttccc tgcatccgca atgttagtgg aagccggagt gtccgacgcc aaggttacct
     2821 gtaccgcgtt gcctgctgga ggcgtcacta ctggaggggt tggcggaggt gtccccggtg
     2881 tcggagttgg tgttggggtg ggtgttgggg caggagctgc tgcaagcaac atgtttgctt
     2941 tcgctctcga tcctgcgcca aagaacccgg tacctgctgt taatcctaat ggtgtcaaaa
     3001 tttcagaagc gtatttgttc tggaatttta cgacacctgt tctggtgata ggcccaaagt
     3061 actgtgtttc agaccctgga gacccggcgc cagaacttgc taactgcgtg tcggcagaag
     3121 agttcaacag aatctggaga tacattacgt cctgtccaga cataccctgc gagaggtttc
     3181 ccgtaaaggt aaagcctgca ggaattcctg aaagtcctcc tcctgtcgaa ccgccagcca
     3241 acgtagccaa ttgtgtttgc aattgcgcaa tggtggcaag caacgatgaa atctgcgcct
     3301 gcaactgctc aatggtttga gcttgagcta caggaggcat tgcaaccgtt gcaaccatta
     3361 ctgcggcaac cgcacccaca aaaatctttt ttgcgattgt actcatctta tttgtttttc
     3421 tgaatttggt tttttaagta tatgttcgac aatactagtt gaagttttcg acctttaggt
     3481 ttttccaatc ccacaaaaag gtgggttcaa ccttcaccat gaatatctac cgaaatcagt
     3541 aacagttttt ccaatgcgag ctcgtaattc ccttgctcaa catctgcttt cgcgctttca
     3601 aataaggcga gctgcccttc cgtgagactt cgcgtttcca aatcggcaaa ctcatattcc
     3661 acgattttct tcgtggcatt ttccagactt tctgcgtgct cctctccaat ttttgcgcca
     3721 agaatctttt cgatttccgc tttttccttt tgtaattgta caatctggct tcccgcctgt
     3781 aaagccttat ccggctggtt ttccacaatt aaggtaacgc tctgggaaac ctgcgtggcg
     3841 tttgcctcgt aaatcttgat tgcggaagcg aggtttcgta tcttattctc ctctgccatc
     3901 cttctcaaat ctgccaaatg tttctgggca agttcaatct gtaaaaacac cttttcctcc
     3961 tcggcagaga agctcaaagg aacctgctcc accgtagtcc gcagggagta taaggcgtcc
     4021 cccggaaggc tgcgtaaact cacttgccac agcaaagctc cccctacagc caaaaatacc
     4081 agtacaggaa ctacgaggac aggcttgcta aaggcaaatt tgaacaaaaa ggcagtatgc
     4141 caaagcatac cagagaagcg ctctttttgc aagttaaaaa agctcttttt gggtaccctg
     4201 tccccgagca attcctgcct tgtcgaaaaa acccattctt ctctgggttt cacgcttttc
     4261 aaaagcgaaa tcttagaaag aagctctttt tcttccattg ttattatgtc ggaaaagcgc
     4321 aggttttgtt acactaggac aaaaactata ctgggacaga tggggcatct tttggaacct
     4381 cgagtttccc ccgaagagct tccatggccc gatgcaagct tacccttacc gaaccctctg
     4441 atttcccttc gagctgggca atttcgggaa tagaaagctc gtctaagtac cgccagataa
     4501 taaggttttg gtattcgtct cgcagattgg ctaaagcctg ctttacctct tctatttccg
     4561 aaagggtcgc tgctttttcg agcaacgaat gttctcttgc gacaagtcct tcggcttcct
     4621 caatacggac aatccgaaaa ctcgccctat ctcggtagta gtcggcaata atgtggcgcg
     4681 ctacctgata gagaaaagcc tgcatgttct ggatctcctg cttctggaac tcctggaaaa
     4741 cacgagtaaa tacctctgaa caaaggtctt cagcaacttc ctgggaatcc acctttaaaa
     4801 cgataaaccg gtagatcttg ataatatatt ggtcgtatat ttccccaaaa cgctcttcca
     4861 taggggtgaa ctaatgggac attgggcttg ttttccttaa ttgtcctatt tgtcctagtt
     4921 ttattcacac tttttccaca gttttattta agctcataag caaccgcgtt ccactcccct
     4981 ttcctttcta ccaggttttt ttgcaaaagc tcgtctaaat cccttcgcaa ggttcgtttg
     5041 gtaacctgcg gaaggacttt ttgcagttcc catacctggg cctggtgctt ggttcggaga
     5101 aaatcaatga tccgctgctg gcgttctgtc aaatctgccc cctcttgttt gcctggagtc
     5161 tgcctgacta ccctttctct gctcgcctgg ctcaccggtg cctttgcagt cgccacagag
     5221 tgcataggct cctttttggg ctcaaccgcc gcttttgtct cctggaaatc ctcgaagatc
     5281 tcaagaaaat cccggattct cgtgtactcg gtcttcaaaa gggcaaagtt ttccggtttt
     5341 acccaccccc ccttctctgc ggtatctaag tatatcaata ggacatcgag ctctctctct
     5401 aaacgaggaa gaagctcctt tttttgctcg cctgaaacaa attccttatc tgtcagcaat
     5461 agaagatcaa ccaaaatagc attggctgtc tcccttattt cctcggtaac cttttcgtct
     5521 gcgggtaagg catccgccac tcgatgagcc gcaaaagcaa gacgcacaaa aaagtttcgg
     5581 ttcattactt gaagatcact tttccacagt atatcagaag caagggggtg ggtcaacgaa
     5641 acccttgaca a
//
LOCUS       DASRUP010000083         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1976388, whole genome shotgun sequence.
ACCESSION   DASRUP010000083 DASRUP010000000
VERSION     DASRUP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976388"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFE94_03360"
     CDS             <1..662
                     /locus_tag="VFE94_03360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZX50156.1"
                     /translation="NNGSGSFAELAVADINKIAPRPKMLNYVETASLPLAGVSAWQGL
                     AEHIKAASGQKVLIHGGAGGIGAFSIQIAKHLGAYVATTVSTADMQYAKELGADEVIN
                     YESQHFWDLAHDYDAVFDASGSGPETYEKSFEVVKRGGIVVSMTEQPNNELAQHYGIT
                     TMRQSTDVTRQRLEKVAQLVDQGVLKVEVDKVFPLEQAAQALTYLQNQHSKGKVVIKI
                     K"
     gene            668..1105
                     /locus_tag="VFE94_03365"
     CDS             668..1105
                     /locus_tag="VFE94_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX50157.1"
                     /translation="MKSINPYLGFNGNCEEAFTFYKSVFGGEFSGMQRFREVDDEEAK
                     KVLKPEEGEKIMHIALPMGKDNFLMGSDSPEALGETKFGNHISISVHADSKEEADKLF
                     AGLSAGGQVTMPMADMFWGDYFGMCTDKFGFQWMVNYSQTQQK"
     gene            complement(1187..1468)
                     /locus_tag="VFE94_03370"
     CDS             complement(1187..1468)
                     /locus_tag="VFE94_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50158.1"
                     /translation="MLKKLAIMGVAVFFVLGAASSALAAGPSEHASCMGHEASAISPP
                     GSSEEFPNGMPGLRAFIGENFPVPPGVIYSTIAKLHEGSHEACDEALEG"
     gene            1562..1957
                     /locus_tag="VFE94_03375"
     CDS             1562..1957
                     /locus_tag="VFE94_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HZX50159.1"
                     /translation="MNNTKLVSWVLRIAVAGEFIGHGVFAIQGKQAWIEWFAAFGVGD
                     PEVAKQLLFLVGIVDIVLAVLVLIRPIPLALLWMAIWGFWTALIRPIVGEPIWDFIER
                     SANWGAPLALLLIVGLPKSFKGWLKGGKS"
     gene            1973..2203
                     /locus_tag="VFE94_03380"
     CDS             1973..2203
                     /locus_tag="VFE94_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HZX50160.1"
                     /translation="MAKKNIHTVYNAGGEKWKNKEEGLQKPLSSHRKKDPAVGKGEQV
                     AKKKRVEHLIHGKKGKIQSRNSYGNDPFPPRG"
     gene            2226..2426
                     /locus_tag="VFE94_03385"
     CDS             2226..2426
                     /locus_tag="VFE94_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50161.1"
                     /translation="MSIFDDYIAYAKDNPKGHWFKRKLFGWGWTPVTRQGWAVVHKRF
                     ELFSRPVGYGRYLSALAVKIIE"
     gene            complement(2458..2907)
                     /locus_tag="VFE94_03390"
     CDS             complement(2458..2907)
                     /locus_tag="VFE94_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HZX50162.1"
                     /translation="MKRYSPFLTVPVLGLAMAALFTVGQASAGGRPLDANLTGAAEVP
                     GPGDPDGSGSAHITLNQGQGEVCFEIAVQNISLPATGTHIHAAPAGVAGPVVVPLTPP
                     DASGTSSGCVDADEELIKAIRQNPEAYYVNVHTTDFPAGAVRGQLSK"
     gene            3077..3277
                     /locus_tag="VFE94_03395"
     CDS             3077..3277
                     /locus_tag="VFE94_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50163.1"
                     /translation="MQNVKGAMDHLRTHQSYPATKAELVATCNSLSDFSGEDKQEFES
                     KLPEGTYNSAEEVAEALGLPKE"
     gene            3370..3957
                     /locus_tag="VFE94_03400"
     CDS             3370..3957
                     /locus_tag="VFE94_03400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX50164.1"
                     /translation="MDDSEQTTQDTYNRIATEWAREHDTPDYWQIEFDIFSKLLPKGK
                     VLDVGCGAARDYPLFHKGGYDYTGIDYSEGLLREAKKKFPDARLERADILRLSFENDF
                     DGFWAVASLLHIPKAKMRLALENVKRAIKPGGIGMVVLKKGQGEKMEQDQRFFAYWEK
                     EEFEEVLARAGFRMLYFVERPVSERTTWLAFYVTL"
     gene            3963..4045
                     /locus_tag="VFE94_03405"
     tRNA            3963..4045
                     /locus_tag="VFE94_03405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3996..3998,aa:Ser,seq:tga)
BASE COUNT         1124 a          874 c         1106 g         1026 t
ORIGIN      
        1 taaacaatgg gtcaggttcc tttgccgagt tggcggtggc agatataaac aaaatagctc
       61 ccaggccaaa aatgcttaat tacgtggaaa ctgccagttt accgttggct ggggtaagcg
      121 cgtggcaagg ccttgcagaa catataaaag ctgcgagtgg gcagaaagtc cttatccacg
      181 gcggagctgg cggcattggt gccttttcta tacaaattgc aaaacatttg ggggcgtatg
      241 tggcaacaac cgtaagtact gccgacatgc agtacgcaaa agagcttggg gcagacgaag
      301 taataaatta cgaatcccag cacttttggg atttggctca tgactacgac gcggtattcg
      361 acgcgtctgg cagcgggcct gaaacctacg aaaaatcgtt tgaggtggta aaaaggggcg
      421 gcattgtagt ttccatgaca gaacagccaa acaatgaact ggcgcagcat tacggcataa
      481 ccactatgcg acaatccacc gatgttactc gccagcgttt ggaaaaagtt gcccagcttg
      541 tggaccaagg cgtgctaaaa gtagaagttg ataaagtttt tcctttagag caagcggccc
      601 aagcccttac ctatttgcaa aatcagcatt ccaaaggcaa agtcgtaata aaaataaaat
      661 aactcttatg aaatcaataa atccatattt aggttttaac ggcaactgcg aagaagcgtt
      721 taccttttac aaatccgtgt ttggcggaga gttttccggc atgcagcgct ttcgcgaggt
      781 tgacgacgaa gaagcaaaaa aagtgttaaa gccagaagag ggcgaaaaaa ttatgcatat
      841 tgccctgccc atgggcaaag ataattttct tatggggagt gacagcccgg aagcattggg
      901 ggaaacaaag tttggcaacc acatttccat ttctgttcac gcagacagca aggaggaggc
      961 ggataaactg tttgccggcc tttctgcggg cggacaagtt accatgccta tggccgacat
     1021 gttttggggc gactactttg gcatgtgcac cgacaaattc ggctttcagt ggatggtaaa
     1081 ctacagccaa acccagcaaa aatagcaaga caaaagagta aatgaaagta tacataaaac
     1141 acacctcgta cgcggaggtg tgtttttagt gcatttgccc tgcaggctaa ccttctaaag
     1201 cctcgtcgca ggcctcatgg gaaccctcgt ggagtttggc aatcgtactg tagataacgc
     1261 ccggcggcac agggaaattc tcgccaataa aagctcttag cccgggcatt ccgttgggaa
     1321 attcttccga tgatcccgga ggagaaattg ccgaagcttc gtggcccatg cagctggcgt
     1381 gttcggaagg tccggctgcc agagcagagg aggcggcacc cagtacgaag aatacagcca
     1441 ctcccattat tgctaatttt ttaagcatcg ttttgctggt ttattaactc ttttttgttt
     1501 cgacttttac tctaactttt cataataaac gcttctaaag catttgtgtt acaattgacg
     1561 aatgaacaat acaaaacttg taagttgggt tttacggatt gccgtggctg gcgaatttat
     1621 tggccatggc gtgtttgcaa ttcagggaaa gcaagcctgg attgagtggt ttgctgcgtt
     1681 tggagttggt gaccccgagg tagcaaagca actacttttt cttgtgggta tcgtggatat
     1741 tgttcttgca gttttggttt tgataagacc gatccccctt gcacttttgt ggatggcgat
     1801 ttggggtttc tggacagcct taatacgccc cattgtaggg gaacccattt gggattttat
     1861 tgaacggtct gcgaactggg gagcaccgct tgcgcttttg cttatagtgg gcttgccaaa
     1921 atcgtttaaa ggatggttga aaggtgggaa atcttgatat aataaaaata tcatggcaaa
     1981 gaagaatata cataccgtgt ataatgctgg aggggaaaag tggaagaata aagaggaggg
     2041 cttacaaaag ccgctttcct cgcaccgcaa aaaagatcct gcggttggca aaggcgagca
     2101 agttgcaaag aaaaagcgcg tggaacacct tatccacggc aagaagggca aaatccaaag
     2161 ccgcaacagc tacgggaacg atcctttccc gccccgcggg taaggaaaac gcggtagaat
     2221 gaaatatgag catttttgac gactatattg cgtacgcgaa ggacaacccc aagggacatt
     2281 ggtttaagcg aaagctgttt ggttgggggt ggacgccggt aacgcggcag ggctgggctg
     2341 tggtgcataa acgcttcgaa ttatttagtc gccctgttgg ttatgggcgt tatcttagcg
     2401 ctttggcagt aaaaattata gagtaaacaa agcgccccct tttaaggggc gttttgttta
     2461 ctttgagagt tggccgcgga ctgcgccggc gggaaagtcg gtggtgtgga catttacgta
     2521 gtaggcttca ggattctgcc gaattgcctt gatgagttct tcgtctgcgt ctacgcagcc
     2581 agaggaagta ccgcttgcgt caggtggagt aagcggaact accacaggcc ctgctactcc
     2641 ggctggagct gcgtggatgt gcgttccggt agccgggagc gaaatgtttt gcaccgcaat
     2701 ttcaaaacac acttcgcctt ggccttggtt tagggtgatg tgggccgagc ctgatccgtc
     2761 agggtcgcct gggcccggaa cttccgcagc tcctgtaagg tttgcatcga gcggccttcc
     2821 gccggccgaa gcctggccta cggtaaacag agcagccata gcgaggccca ataccggtac
     2881 cgtgagaaag ggggagtatc ttttcataag tgcatggata aaacttttta taactattcg
     2941 accctagtaa taacgtatgg agaataaaaa ggtgttacgc attccaacat tcattgactt
     3001 ggtcgcttgg gtatagtaag ttaagtgtag agtaagggtc ggttgtaata aacaataata
     3061 aaaccgtact atagttatgc aaaacgtcaa aggagccatg gatcacttgc gaactcacca
     3121 gagttaccca gctacaaaag ctgaacttgt cgcaacttgc aacagccttt ctgatttttc
     3181 cggagaggac aagcaagaat tcgaaagcaa gcttcccgag ggaacctaca attccgccga
     3241 agaagtcgcg gaagccctgg gtttgccgaa agaataaagt ttcgcagtac tcggacaaaa
     3301 ccatccttca agggtggttt tgttttgtga gtgcgttgtc aaggagctta ttttatgcta
     3361 ttttgaggca tggatgattc agagcagaca acccaagata cgtataatcg cattgcaaca
     3421 gagtgggcca gagagcacga tactccagac tattggcaaa tagagtttga tattttctca
     3481 aagcttttgc ccaaaggaaa ggttcttgat gtggggtgcg gcgctgcgcg cgactaccct
     3541 ctttttcaca aaggtggtta cgactatacc ggaattgatt attcagaagg ccttttaagg
     3601 gaggcaaaga agaaatttcc cgatgcaagg cttgagcgag ccgacattct gcgcttgtcg
     3661 tttgaaaacg atttcgacgg attctgggct gtggcttcgt tactgcatat acccaaggca
     3721 aaaatgcgcc tggcgttaga gaacgtgaaa agagcaataa agccgggcgg tattggcatg
     3781 gttgtattga aaaagggcca gggggaaaaa atggagcaag accagcgttt ttttgcctac
     3841 tgggaaaagg aggagttcga agaggtgctg gcccgggccg gatttcgtat gctctacttt
     3901 gtggaacgcc ccgtttcaga acgtacaacc tggcttgcgt tttacgtaac cttgtaagtg
     3961 ttggagggtt ggcagaacgg ctaatgcgcc ggtcttgaaa accggtggga gcaatccctt
     4021 gggggttcga gtccctcacc ctccgcttat aacaagtaat aagaactatt ccataataag
     4081 cgtacataac atatgcaaaa caagattttt atcgccattg cgggggccgc
//
LOCUS       DASRUP010000084         6625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_382502, whole genome shotgun sequence.
ACCESSION   DASRUP010000084 DASRUP010000000
VERSION     DASRUP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382502"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VFE94_03410"
     CDS             <1..1206
                     /locus_tag="VFE94_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50165.1"
                     /translation="CQDGTCDSEEAGSGTSPGNTADRTTYCLSTSGNGGAQAAKDTAG
                     NVGVAGCVVISYTPPSVSAYVALYTSGGSEVSGSEVSSTSATWTRVRSSAITLSDATD
                     YVVRIKADSNVTAEIANAKIIFEQTDAGGIDELEPVHQYINTLATETSASYVLQDFDN
                     EYNPSNWAGGTFAYLFEATLHGSVNPDTAAAQLYNVSDSEAIDDTAGGGDVESEVTTT
                     NGSYERKRSLDLSGNSDWPSAAKTLDTQLNNSGGTTSTASSWLIIQVTGLAPAAVISV
                     VVVDGAVVYGTVATSKNTTSGDLNDGQDGVNNGDTTENFNIKGQNATGGGCTWTLSDT
                     AGSDTYVHEFSTNSGTDWLDLSTSYRQLVAGVSTSATQSFDLRITVPTPSSCFGSQTV
                     DVTVQAVQG"
     gene            1211..3337
                     /locus_tag="VFE94_03415"
     CDS             1211..3337
                     /locus_tag="VFE94_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZX50166.1"
                     /translation="MIRAGEILAEHIRVGLVLVLFTPLLVGPIGVTFGEYPKVLFFRT
                     LVEVLLIFYVILVLRRPEYLPKNTLLLWAVVGFVVVVSLSAITGIHFERSFLGDLDRS
                     EGVLLYLHLLALFVVAAGILQRYTDWLVLFRWAVGLSGVVAIVGFLQRLNVIDLYSVS
                     GPYPAGTFTNTAFYASWLVIIFFLGLFLFFAESEWRRKAVWAAISIANGVVLLLTFSL
                     GAWVGAAAGIGFLVLAKGGKVFWASVCLFFVFGLTLFFAFGTALEADIINSLETRLPV
                     WDNAFKAWQERPLLGWGPESFSYVFELNRPESLLSIAAFFDRPHSKPLEILVHNGLVG
                     LAAYVFLFGVSGYYVLRSRDTLAFWPKVSLLALLLAYFAQNLFLFDTVSTLLVFFLLL
                     AFFSTVVLPQLRFSLPVPRMAKIPAYPLVGCLVLISLLSLYFVNLQPLKQNLLLVQGS
                     RFEPHDVGTALVYYQKALEQETLYQKDLEVFVTERLLTYMDEPWAQSAQLHIVALLAG
                     VVPTLQERTEKPDKIHRLYYVLLGGIRDKQYRTFGDTRFLAELEGILRKGVAAYPNRP
                     SFYLLLGRAKILQQQYEEGEAYFWEAYARSEGQPLAKARLLYKRIGGAYVEAREMERA
                     AEYFMKVWEIDQAWAKTNPEQKELFLVAGGERGFVEQVAHFHFIELGDLQTAKSIYEQ
                     AIEVYSGEDKDWFQSQYDLFLSKCCG"
     gene            3421..3915
                     /locus_tag="VFE94_03420"
     CDS             3421..3915
                     /locus_tag="VFE94_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50167.1"
                     /translation="MKSYSTIISVAVWAGLLIAAGAYAAQTGVVSATVTAQNISVAVQ
                     DASIAYGSLALSASYSTLAAEANDLQFASNIGNLTEDFNIQGADTASWTLESAIGSEQ
                     YTHEFSTNRGTVWVKLSTSYQQLVDAVAQNVSQQFDLQITVPSGTSDFTQQTAAVTVQ
                     AVAD"
     gene            3936..4706
                     /locus_tag="VFE94_03425"
     CDS             3936..4706
                     /locus_tag="VFE94_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50168.1"
                     /translation="MRTLFFVGLLVCVIPGVTFARVGVGVGVGKIQLDEPLRRGGIYE
                     LPNLPVINTGTEPGEYEARIEYHHNQPELVPPREWFRFEPTRFILEPGEVQQVKVLLT
                     LPIDEAEPGDYFAYVEGRTVSKQEDGTTHVGVAAAAKLYFSVIPSNKWDATLYRLSLL
                     WDRYSPWPQVVVAVLGLIIFLFVARLMFRRFFSLNLRVKGKESAGLPDKKGVDTIDEE
                     EILRLAEELKKKRRVAVVKRASTRKAATRKTTTRKKPE"
     gene            4710..5480
                     /locus_tag="VFE94_03430"
     CDS             4710..5480
                     /locus_tag="VFE94_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50169.1"
                     /translation="MQKLLYVIAVIVFLGMGGLFWHSHSSAQSDTLQTTVRITVCGND
                     VQESGEQCDGTDLAGESCTTQGFTGGTLSCDPACDFDTSSCTSTPGGGGGGGGGRVPA
                     TVSEPATQINFTGQASPGDTVFLLKDAQLAGTAVVGPDGVFSFSLRELSGGSYIFSLY
                     SQSPSGERSSFVTLPVQITPHTVTNVSGIVFDFEKPIDSAQARCADPNNDSHVDLVDF
                     SILLFWFERSAVPTHIDCNGDGKADLVDFSIMAYYWTG"
     gene            5500..6360
                     /locus_tag="VFE94_03435"
     CDS             5500..6360
                     /locus_tag="VFE94_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HZX50170.1"
                     /translation="MIIKFIIFIYLFLVVPFWADAAEFRVESDAESFGVGEEFLVTLV
                     VDTAGEAINAFEGKVVFPESLLELQGVREKGSVVSLWVQDPPSFSGLTPGGYNGRGVL
                     FSLVFKVKGEGSGIIDVEQGRALLNDGEGTPADLTISRFEFSIPNEPSISQNPMVETN
                     DTEPPEPFEIRIARDTSVFDGKPFLVFAAQDKNSGIDHYEISFLYKGKTTDWQLAENP
                     FLLEHEEDLEKIWVKAVDRAGNERIATLALGTKSSFLVFLLWGNAGILIGIGIVLIFL
                     LWRKKEKREK"
     gene            6364..>6625
                     /locus_tag="VFE94_03440"
     CDS             6364..>6625
                     /locus_tag="VFE94_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50171.1"
                     /translation="MQNSKYYKCYKSYNYYKPFFFFVVFLFGFSFFYLPEANAATLSL
                     QPSTGTFQVGSTFEVSVYIDTQKETINAIEAYLHFPADKLQLV"
BASE COUNT         1583 a         1450 c         1795 g         1797 t
ORIGIN      
        1 tgtcaagacg gcacttgcga ttcagaagag gcaggcagcg gcacttcgcc gggaaacacg
       61 gctgacagga ctacttactg tttaagcact tccggtaatg gcggcgcaca ggcggcgaaa
      121 gacacagctg gcaatgtcgg cgttgccggc tgcgtggtaa ttagttatac tcctccttca
      181 gttagtgcgt acgtggcgct ttacacgtcg gggggcagtg aagtgtcggg tagcgaggtg
      241 tcttcaacaa gtgcaacctg gacaagggtg cgttcttctg ccattacgtt aagcgacgcc
      301 accgattatg tggtgcggat aaaagcggat agcaacgtta ccgcagaaat tgccaacgcc
      361 aaaatcattt tcgagcaaac tgacgcgggc ggaatcgacg aattggaacc cgtgcatcag
      421 tatattaata ccctggcaac agaaacgagt gcctcttacg tacttcaaga ttttgataat
      481 gaatacaatc cgtccaactg ggctggagga acatttgcgt atctttttga agcaacgctt
      541 catgggagcg taaatccgga tactgctgct gcccagcttt ataatgttag tgattccgag
      601 gctatagacg ataccgcggg agggggagat gttgagagtg aggtgaccac aaccaatgga
      661 agctatgagc gcaaacggag cttagatctt tctggtaaca gcgactggcc gagtgctgca
      721 aaaactctag acacgcaact taacaatagc gggggcacaa cctctactgc ttcttcctgg
      781 cttattatcc aggtgacggg gctggcgcct gctgcggtaa tttccgtggt ggtggtagac
      841 ggggcagtgg tgtatgggac agtggctacc tcgaaaaata cgacttccgg ggatttgaac
      901 gacggccagg atggggtgaa caacggcgac actaccgaga atttcaacat taaagggcag
      961 aacgccacag gcgggggctg cacctggaca ctttcagata cggcaggaag cgatacctat
     1021 gtgcacgaat tttccacaaa ctcgggaacc gactggctcg atttaagcac aagctatcgg
     1081 cagcttgtgg ccggtgtttc aacgagcgca acgcagagct tcgatttgcg cattaccgtg
     1141 cccacacctt ccagttgttt tggctctcaa actgttgacg taactgtgca ggcagtgcaa
     1201 ggttaaagaa atgatacgcg cgggagaaat tttggcagaa cacattagag tgggactcgt
     1261 acttgtcttg tttactcctc tcttggtggg gcccatagga gttacttttg gggagtatcc
     1321 caaggtgctt ttttttcgca cgctggtcga ggtacttttg attttttatg tgatcctggt
     1381 tttaagaaga cctgaatacc tgcccaaaaa tactcttttg ctttgggctg ttgttggttt
     1441 tgtggtagtg gtgtccttga gcgccattac tggcattcat ttcgaacgaa gttttctcgg
     1501 cgacctcgac cgttcggaag gcgttttgct gtatctgcat ttgcttgcgc tgtttgtggt
     1561 ggcggccgga attctgcagc ggtatacgga ctggcttgtt ttgtttcgat gggctgttgg
     1621 gctttctggg gttgtcgcta tagttgggtt tctgcagaga cttaacgtta tagacctgta
     1681 ctcggtttcg ggcccttacc ctgcgggaac gtttacgaat actgcgtttt atgcctcctg
     1741 gcttgtcatc atttttttcc ttggactttt tctgttcttc gcagaatctg agtggcgaag
     1801 aaaagcagtg tgggcggcca taagtattgc aaacggcgtt gttcttttgc tcacattttc
     1861 tctgggagcc tgggttgggg cagctgccgg catcggtttt ttggttttgg caaagggtgg
     1921 gaaagtattt tgggcgagcg tatgtttgtt ttttgtgttc ggtttgacct tgtttttcgc
     1981 gttcggcacc gccttggaag cagacattat aaacagtttg gaaactcgtc tgccggtctg
     2041 ggacaacgcg tttaaagcat ggcaggaacg ccctttgtta ggctgggggc cggaatcttt
     2101 cagctatgta tttgaactga accgccccga gtctttactt tccattgctg cgttcttcga
     2161 ccgaccgcac tcaaagccgc tggaaattct tgtgcacaac ggccttgtgg ggttggcggc
     2221 ctacgttttt ctttttggcg tgtctggata ttatgtgttg cgttcaagag atacgcttgc
     2281 tttttggccc aaggtttcgc tgcttgccct tttgctggcg tattttgcac aaaacctttt
     2341 cttgtttgat accgtgtcca cgctgctggt attcttcctg ctactcgcgt ttttcagcac
     2401 tgtggtgttg ccgcagttgc gcttttcgct tcctgttccc cgaatggcaa aaataccggc
     2461 gtaccctttg gtggggtgcc ttgttttgat ttcgcttctt tctctgtatt tcgtgaatct
     2521 acaacccctt aaacaaaatc ttttgttggt gcaaggctcg cgtttcgaac cgcacgacgt
     2581 gggaacggcc cttgtgtact accaaaaggc attagaacaa gaaacactct atcaaaaaga
     2641 tttggaagtt ttcgttaccg aacggttgct tacctatatg gacgaaccct gggcgcagtc
     2701 ggcccaattg cacatagtgg ctttgttggc tggcgttgtc cccacattgc aagagagaac
     2761 cgaaaagcca gacaaaattc atcgcctgta ctatgtattg ctgggtggca tccgggataa
     2821 gcaataccgc acatttggag acactaggtt tctggccgaa ctcgaaggca tcctgcgtaa
     2881 aggagtcgcg gcgtacccca acaggccgtc attttacttg ttattgggcc gcgccaagat
     2941 tttgcaacag caatatgagg aaggcgaagc ttacttctgg gaagcgtatg cgcgttctga
     3001 aggccagccg cttgccaaag ccaggctttt gtacaaaaga ataggaggag cttatgtgga
     3061 ggcgagagaa atggaaaggg ctgcggaata ctttatgaag gtttgggaga tagaccaggc
     3121 gtgggcaaaa actaatcccg agcaaaaaga actctttttg gtggcaggag gggaaagggg
     3181 ttttgtagaa caggttgcgc actttcactt cattgagctg ggtgatctcc aaactgcaaa
     3241 gagcatctat gagcaggcca ttgaagtata ctcgggagaa gacaaagatt ggttccaatc
     3301 gcaatacgat ctgtttcttt ccaaatgttg tggataactt tgtttggagg ataagacaag
     3361 taggcatata ataaagcata ccttaaaagg tcgcctagaa ggcacgcaga catacattct
     3421 atgaaatctt attctaccat aatttctgtt gcggtgtggg caggtttgct gattgcagca
     3481 ggagcgtacg ccgcacagac cggcgtcgta tctgctacgg taaccgcgca aaacatttct
     3541 gttgccgtac aagacgccag cattgcatac ggctctctcg ccttgagcgc gagctatagc
     3601 actcttgcgg cggaagccaa cgacttgcaa ttcgcttcga acatcggaaa tcttaccgaa
     3661 gactttaata ttcaaggggc cgataccgca agctggaccc tcgaaagcgc aattgggtct
     3721 gagcaataca cccatgagtt ttccacaaat cggggaactg tctgggttaa attgagcacg
     3781 tcgtatcagc agttagtaga tgcggtagct caaaacgtga gccagcaatt cgacttgcaa
     3841 attacggtac cgagtggcac gtctgacttc acgcagcaaa ctgccgctgt aacggtgcaa
     3901 gcggttgcag actaacgcac ttgagaatct ttattatgag aacccttttt ttcgtggggt
     3961 tgttggtatg tgttattccg ggcgttacct ttgcaagggt tggtgtggga gttggggtgg
     4021 ggaaaatcca gctcgacgaa cctctccgcc ggggaggaat ttatgaactc ccaaatcttc
     4081 cagtaatcaa taccggaacg gaacctggcg aatacgaagc acgtattgaa tatcatcata
     4141 accagccaga actcgtacct ccgcgcgagt ggtttcgttt tgagccgact cgttttattt
     4201 tggagcccgg tgaagtgcag caggtaaaag tgctgcttac gctccctatc gacgaggctg
     4261 aaccaggcga ttactttgca tatgtggaag ggcgtaccgt aagcaagcag gaagatggca
     4321 ccactcatgt aggagtggca gcagctgcaa agctatattt tagcgtaatt ccttcgaaca
     4381 aatgggacgc gactctctac cgcctttctt tgctttggga tagatattct ccgtggccgc
     4441 aggtagtcgt ggcggttctt gggctgatta ttttcctttt tgttgctcgt ttgatgttcc
     4501 gccggttttt ctctttgaat cttcgcgtaa aaggaaaaga atcggcaggg ttgcccgata
     4561 agaaaggcgt tgatacgata gatgaagaag agatactgcg gcttgccgaa gagttaaaga
     4621 agaaacgaag ggtagcggta gtaaaacgag cgagcacgag aaaggcagcc acgcgaaaaa
     4681 caaccacaag aaagaagccg gaatgagtaa tgcaaaaact tctttatgtc atagcggtta
     4741 tcgtgtttct cggaatgggg gggttgtttt ggcattctca tagtagtgcc caatcagaca
     4801 cactacaaac tacggtgcgc attaccgtgt gcgggaacga cgtgcaagag tcgggtgagc
     4861 agtgcgacgg gacggattta gcgggagaaa gctgcaccac ccaaggcttt acaggtggta
     4921 cgctgtcttg cgaccctgcg tgcgactttg acacttcgag ttgcacctct acgcccggtg
     4981 gaggtggtgg aggaggcgga gggagggtgc ccgccaccgt ttcagaaccg gccacgcaaa
     5041 ttaattttac ggggcaggca agccccggtg atactgtttt tttgctcaaa gatgcgcagc
     5101 ttgcgggcac tgccgtcgta gggccagatg gagtgttttc cttttctctt cgcgaacttt
     5161 ctggagggag ctacatcttt agcctgtatt ctcaaagccc gagcggggaa cgctcatcgt
     5221 ttgtaacgtt gcccgtgcag attactccgc acacagttac caatgtgagc ggaattgttt
     5281 tcgattttga aaaaccaata gatagtgcgc aagctcgttg cgccgatccc aataacgata
     5341 gccacgttga tcttgttgat ttttcgatat tgcttttctg gttcgaacgc tcggctgttc
     5401 ctacacacat tgactgcaat ggagacggca aggccgattt ggtcgatttt agcatcatgg
     5461 cgtattattg gaccggctag taagattata ccaggtataa tgattataaa gtttataatt
     5521 tttatatatc ttttcttggt tgtacccttt tgggcagacg cagctgaatt tcgggtggaa
     5581 tcggatgccg aaagttttgg cgtgggtgaa gagtttttgg ttactcttgt ggttgatacc
     5641 gcgggtgagg caataaatgc tttcgaagga aaggtggtgt tccccgagtc tttactcgag
     5701 ttacagggtg tccgcgaaaa aggttcggtg gtgagtttgt gggtgcagga tccgccttct
     5761 ttttccggtt taactcctgg ggggtacaat ggaagaggag tgctgttctc tctcgtgttc
     5821 aaggtaaagg gagaaggaag cggaatcatt gatgtcgaac agggaagagc gctgttgaac
     5881 gacggagaag gaacgccagc agacctgaca atttctcgtt tcgaattttc catacccaac
     5941 gaaccctcaa tttcccaaaa cccgatggtc gaaacaaacg atacggagcc gccagaaccc
     6001 tttgaaattc gcattgcgcg agataccagc gtgtttgatg gcaagccgtt tctggttttt
     6061 gccgcgcaag ataaaaactc cggcatagac cactacgaaa tctcttttct ttacaagggt
     6121 aagaccaccg actggcagct cgcagagaat ccctttttgc tcgaacatga ggaagatttg
     6181 gaaaaaatct gggtaaaagc ggtggatcgc gccggcaacg aacgcatcgc gacattggct
     6241 cttggaacga aaagttcttt tcttgtgttt cttctctggg gaaatgctgg tatactaata
     6301 ggaataggaa ttgtcttgat ttttttgcta tggaggaaga aggagaaaag agaaaagtga
     6361 aaaatgcaaa attccaaata ttataaatgt tataagagtt ataattatta taagcccttc
     6421 tttttctttg tcgtttttct gtttgggttt tcgttttttt acttaccaga agcgaatgcc
     6481 gccacgctgt cgctgcagcc ttccacgggc acgtttcagg tggggagcac gtttgaggtt
     6541 tccgtatata tagatacgca aaaggaaaca ataaacgcaa ttgaagccta cctgcatttt
     6601 cccgcggaca aactgcagct tgttt
//
LOCUS       DASRUP010000085         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2141978, whole genome shotgun sequence.
ACCESSION   DASRUP010000085 DASRUP010000000
VERSION     DASRUP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141978"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..139
                     /locus_tag="VFE94_03445"
     tRNA            68..139
                     /locus_tag="VFE94_03445"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gln,seq:ttg)
     gene            144..386
                     /locus_tag="VFE94_03450"
     CDS             144..386
                     /locus_tag="VFE94_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZX50172.1"
                     /translation="MYFVYLIECEDKTIYTGITTNVERRFKEHTLGKGGAYTRSKKPK
                     KLLYTETHPNRSSALKRESEIKSWPRERKLQLIQSH"
     gene            638..1642
                     /locus_tag="VFE94_03455"
     CDS             638..1642
                     /locus_tag="VFE94_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50173.1"
                     /translation="MTDNSRLKEQIEKIELLKNAERWGSKYSLWEKTTERILQDSLHE
                     DALKLFKQQGTSFLKEDPEDSQKQYLQELDNRKQILEGLLAEAKEYRQNSIYPKAKFV
                     IAKIPPKSLLKKAILASIVFIVILIFVAYIFILTGELPDRINLGVFEYDLSFQTKESP
                     KITYTPQVYFYDIEGVVIPKKSSGLQGGNLNDFSKISIGLTTELISNSPVKITYIDFQ
                     ISASNSDQTSPDSKWVKIDDKDFVLQAGEQKTAMIVEIASKFLSELPTKHFPDSKQIS
                     VSLLTRLRLSNPETDEELARKQIQVICRNGTNLLLANHEEADFSVFYPSCLVMNIKDF
                     "
     gene            1802..2023
                     /locus_tag="VFE94_03460"
     CDS             1802..2023
                     /locus_tag="VFE94_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50174.1"
                     /translation="MENSHETTNEELARMIAEGFHGVDKRFDGIDKRFDQVEERLEHI
                     EKFLLEDHRRRIEKLEQRMQKLEDLLALQ"
     gene            2066..2443
                     /locus_tag="VFE94_03465"
     CDS             2066..2443
                     /locus_tag="VFE94_03465"
                     /inference="COORDINATES: protein motif:HMM:NF018271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50175.1"
                     /translation="MDNILLLGTVFIAVTLVGFYIAYLYGRKTKRFRWREYIAIIIWP
                     VLAVLGFAYFIDTKILSLFIISAVFGFVLEYILGLTYHKALNKRLWSYDRLSVGGYTS
                     LLTLPIWGIAGVVFWFLSKMVGL"
     gene            2564..2803
                     /locus_tag="VFE94_03470"
     CDS             2564..2803
                     /locus_tag="VFE94_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50176.1"
                     /translation="METITIPKRLLKEKDVVVIPRKEYEELLRIHKKHKGFYEELDKD
                     LDKAIKSYKAGQFYGPFKTVQESKSFLASRKRKKK"
     gene            2800..3045
                     /locus_tag="VFE94_03475"
     CDS             2800..3045
                     /locus_tag="VFE94_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50177.1"
                     /translation="MRHIFGKGFVERLENLPKETQQKFWKQLGFLVSDLRHPSLHAKR
                     YDEAKGIWQARVDRSYRFYFLIDEDIYILLDIRAHPK"
     gene            3125..>3708
                     /locus_tag="VFE94_03480"
     CDS             3125..>3708
                     /locus_tag="VFE94_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZX50178.1"
                     /translation="MAFIDEIEIRVKAGKGGDGVVRWLHTKEKEYAGPSGGNGGRGGN
                     VYAKALRDVHLLSRYRANKELAAENGKNGQAKDLHGSNGKDLELLLPVGSLIANKTTG
                     EKFSLNQEGEHILLLSGGFGGRGNKSFQSSRNRSPDKATPGKPGEEAAFFIEVELIAH
                     IGLVGLPNAGKTSLLNELTAAHGKVGDYPFTTLEP"
BASE COUNT         1157 a          762 c          878 g          911 t
ORIGIN      
        1 atagtccagt aggagggctg gattttatga agaaaatatg gtatagttta aatcgtattg
       61 tgaattttgc cgggtcgtct aacggtagga cgtctccctt tggaggagaa tattctaggt
      121 tcgaatccta gcccggcaac actatgtact tcgtctactt aatcgagtgc gaagacaaaa
      181 cgatttatac aggcattaca accaatgtgg aacgcaggtt taaggagcat acgctgggaa
      241 agggcggcgc ctacacgcgt tctaaaaaac caaagaaact tttgtacacc gaaacccacc
      301 caaacagaag tagtgcttta aaacgcgaaa gcgaaataaa gtcctggccc cgcgagcgaa
      361 aacttcagct tatccaatcc cattaaaata tgctaaatac aaaactacta ctcatctgga
      421 atatcgttct tacgctgctg gttgtagttt tgctttgggg aatatctgct ttatcgcaag
      481 agacaaataa cctttcctct cacaatactg cccaaaatgt tgtcctgtgc ggataccttg
      541 aagatatcca agcacagact ggcgtcgata gaacggtatc cccgaactgc caaggagata
      601 tcgaattgat ttatgatcta aatacaaaat aaggcgaatg actgacaact cacggctcaa
      661 ggagcagatt gaaaaaattg aattacttaa aaacgccgaa cgctggggtt caaaatatag
      721 cttatgggaa aaaacaaccg agcgaattct tcaagattct cttcatgaag acgctctaaa
      781 actcttcaaa caacagggta cttcttttct caaggaagat ccagaagaca gtcaaaaaca
      841 atatcttcaa gaactagaca accgcaaaca aatccttgaa ggtcttctag ctgaagcaaa
      901 agagtatcgc caaaactcta tatatccaaa ggcaaaattc gtcatagcaa agattccccc
      961 aaagtctttg ctcaagaaag caatcttagc ctctatcgtc tttatcgtca ttctcatctt
     1021 tgtagcttac atttttatcc ttaccggcga actaccagat aggataaact taggagtttt
     1081 tgagtatgat ttatcttttc aaacaaaaga gagccccaaa ataacataca cgccacaagt
     1141 ttacttctat gatatagaag gggtagtaat ccctaaaaaa tcttccggac tacagggagg
     1201 gaatcttaac gatttctcaa aaatatcgat tggactaaca actgagttaa tcagcaactc
     1261 tccagttaaa ataacataca tcgactttca aatatctgca tcaaatagcg accaaacgag
     1321 tccagactct aagtgggtga agattgacga caaggatttc gttctgcagg caggtgagca
     1381 aaagactgct atgatagtag agatagcatc aaagtttctt tctgaactac ccaccaaaca
     1441 ctttcccgac agcaaacaaa ttagtgtgag tttgcttact aggctacgct tgtcaaaccc
     1501 tgaaacagac gaggagttag caaggaaaca gatacaagtc atctgcagaa atgggactaa
     1561 cctcctatta gcgaaccatg aggaagctga tttttctgta ttttatccgt catgcctagt
     1621 aatgaatata aaagattttt agcatcgcca actcggaggg cgaggggcgc ggcacgtcag
     1681 cacacgcaac gaacggcggc acggcagcga ggcgggaagg tagaacggac gggcacgcaa
     1741 cctaacagca cgacagtttt tggtttgaca ttcaataggg gacaggtaca ataaaaatac
     1801 tatggagaat agccacgaaa caacaaatga agagctggcg cggatgattg cagagggctt
     1861 tcatggcgtg gataaaagat ttgatgggat agataagcgc tttgaccaag tagaagaacg
     1921 cctcgagcat atcgagaagt ttttgcttga agaccatcgc cggagaattg aaaaactgga
     1981 acaacgaatg cagaaactcg aagatttgct cgcactgcaa taatcaaaaa tcgccatttc
     2041 ggcggttttt tggtagagta ggggaatgga taatatttta ctgcttggga cggtatttat
     2101 tgccgtaact cttgtaggtt tttacattgc gtatttgtac gggcgcaaga caaaacggtt
     2161 tcgttggcga gaatatattg ccattattat ttggcccgta ctggcagttc tgggatttgc
     2221 atattttatt gatacaaaaa tattaagttt gtttattata agtgctgttt ttggtttcgt
     2281 tttagagtat attcttggcc ttacttatca taaagcacta aacaaaagat tgtggtcgta
     2341 cgacagactt tcggtaggcg gctacacaag cctgctgacg cttcccatat ggggaatagc
     2401 aggggttgta ttttggtttt taagcaaaat ggttgggttg tgactttacg gttgcgcgac
     2461 gcttcagcgc ggcgggaggg tagaacagac ggacacgcaa gacgtgccag aaagttgatt
     2521 ttctggtttg acgagagcgg aaatggatgt ataataagaa tatatggaaa ctattactat
     2581 ccctaaaaga ctgcttaaag aaaaggatgt tgttgttatt ccccgcaaag agtacgagga
     2641 gcttttgcgt atccataaaa agcacaaggg tttttacgaa gaactcgaca aggatttgga
     2701 caaggcaata aaaagctaca aagccggcca attttacggc ccgtttaaaa ctgtacaaga
     2761 aagcaaaagt ttcttggcct cccgcaaaag aaaaaagaaa tgaggcatat ttttggaaaa
     2821 ggttttgtgg aacggctgga aaaccttccc aaagaaacgc aacagaaatt ttggaaacag
     2881 ctcgggtttc ttgtaagcga cctgcgccac ccttcgcttc atgccaaaag gtacgacgag
     2941 gctaaaggca tatggcaggc acgagtagac cgatcgtatc ggttttattt tttgattgac
     3001 gaagacatct acattctctt ggacattcgc gcccacccga aataaggcaa ctacccctct
     3061 cactacgatc gtgagtatta atgacatagc ttctcgcggg gtggattttt tgatagggta
     3121 aagtatggca tttattgacg agatagaaat tagggtaaag gcgggcaagg ggggagacgg
     3181 cgtagtgcgc tggctgcata ccaaagagaa agaatacgca ggcccctcgg gcggaaacgg
     3241 gggccggggc ggaaacgtgt acgcaaaggc tttgcgcgac gtgcatttgc tttcccggta
     3301 tcgcgccaac aaagagcttg cggcagaaaa cggcaaaaac gggcaggcaa aagatttgca
     3361 tggatccaac ggcaaagacc tggaactttt gctgccggtg ggctctctta ttgccaacaa
     3421 aactaccggg gagaagttta gcctaaacca ggaaggcgaa cacatactgt tgctttctgg
     3481 cggatttggc ggcaggggga acaaatcgtt ccaaagttcg cgaaaccgct cgccagacaa
     3541 agccaccccg ggaaagccgg gagaagaggc cgcctttttt attgaggtgg aacttatcgc
     3601 gcatattggg ttggtgggct tgcccaacgc gggcaaaaca agcttgctca acgaacttac
     3661 ggcggcccac ggcaaagtgg gggattaccc gtttaccacg ttggagcc
//
LOCUS       DASRUP010000086         9026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136 k141_6671,
            whole genome shotgun sequence.
ACCESSION   DASRUP010000086 DASRUP010000000
VERSION     DASRUP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6671"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFE94_03485"
     rRNA            <1..659
                     /locus_tag="VFE94_03485"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            893..964
                     /locus_tag="VFE94_03490"
     tRNA            893..964
                     /locus_tag="VFE94_03490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:925..927,aa:Ile,seq:gat)
     gene            1014..1628
                     /locus_tag="VFE94_03495"
     CDS             1014..1628
                     /locus_tag="VFE94_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX50179.1"
                     /translation="MEKFPEHEPSVEGSSDEKAFQAYVEDLDLSPEDFNKKILDVGSG
                     SSQFAKWAKDHQVSSEIYSIEPYQQPQEISKSLQGRAETMPFQDNAFDLVVSNASIPN
                     VFLGEAPEVMKEKVRDSLVGFMRVVRPGGEIRLGRVLKGNVYESQQNISAAVEEVLEE
                     LRQAYGAEVEEIRYSAGDTYEYDAKQEPIRILARAYLIKIRKPN"
     gene            1706..1777
                     /locus_tag="VFE94_03500"
     tRNA            1706..1777
                     /locus_tag="VFE94_03500"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1738..1740,aa:Ala,seq:tgc)
     gene            1855..2382
                     /locus_tag="VFE94_03505"
     CDS             1855..2382
                     /locus_tag="VFE94_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50180.1"
                     /translation="MFNPEREPNTGEEQEGGSDYGESYEQGIRRNEEGIHSTPEGEEI
                     LKGMARKARGEEPQDNRHDQGQEEEVVKITNEDIWRNKIERRADLIDLTRPIPISEED
                     PTSFPPGILRDLREEIFRLRAEGKGGMDIYRELSKFTYPDLYRGYDTKVQEVAKLYNQ
                     ALNSLVRSKEVDTTE"
     gene            2927..3166
                     /locus_tag="VFE94_03510"
     CDS             2927..3166
                     /locus_tag="VFE94_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50181.1"
                     /translation="MRITIGQADKARGSLLKLLVQKIDIVEYVAMTRIERVKTYDSGA
                     PCPQDSCIAVRLCKPLPEGLVFPPEVDGVQVFVEN"
     gene            3307..3675
                     /locus_tag="VFE94_03515"
     CDS             3307..3675
                     /locus_tag="VFE94_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50182.1"
                     /translation="MRIRYEVVALRTGLAFVFLYTAVVGFMDPGAYIKWIPGFVLESL
                     PVGAVPFLISFFIFEAVLAVWLLWGKWGVAAAVISAALLFAITFLNLNAFGVLFRNVG
                     LIAAAVALGFMEGKGQTRNR"
     gene            3874..4680
                     /locus_tag="VFE94_03520"
     CDS             3874..4680
                     /locus_tag="VFE94_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX50183.1"
                     /translation="MTGKKKYILLIEDEATLSNLIESGLRDQGYEVKAAKDGNEGLRL
                     IRETKPDLVLLDIMLPGLKGFDILEKLHKEDGVLPGLPVIIISNSGDSIEIERALHMG
                     VRDYLVKVNFNPNEVVEKVNSVLEAEADKKKKKPVKLQPVKGNRVLLVEDDTILSDAL
                     ERKFVEKKYTVLKALNAALARRVLEEHEVDVILLDLVLPDEHGLSFLRQLKKNDATKD
                     IPVIITSNLGQQEEIDEGLKAGAVDYIVKTNTVPGEIFEKVQALVKKGQD"
     gene            4704..5903
                     /locus_tag="VFE94_03525"
     CDS             4704..5903
                     /locus_tag="VFE94_03525"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HZX50184.1"
                     /translation="MAIYAGIGLSKEKDAFQAGYAACKTAMEAAGGKTPELTIVFASV
                     SFGQPEVLKGVREASGGAPLVGCSDAGEITNEGPSQHSVGVMAISSDQIDFYTGLGRD
                     VKKGAREAGQAVAKEVKEKAKDPLQAFIMFPDVLTGNGADIVRGALDVLGEHFPVVGG
                     AAGDDFLFEKTYEYQDEEAAWGAVGGVGLAGKFSMGAGVRHGWVPIGVPMKVTKAAGS
                     VVHELNGRPAVSIYEDYFGDKAGELRKEPLARMAITYPLGIKLPGMDEYLIRDPITVD
                     EQGAITCAAEIPEGSEIRLMIGSTEKAVEAAQDAARKLMADFEKQGSQPKFVLMFNCI
                     AREKLFGQRANEEIQAVMEIIGKDVPLLGFYTYGEQAPIGGELHDTEKISSRFYNETV
                     VMFAVGE"
     gene            5919..6866
                     /locus_tag="VFE94_03530"
     CDS             5919..6866
                     /locus_tag="VFE94_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZX50185.1"
                     /translation="MDIFGVQTLVFGWLLLIVSGAVLASRAVAVYRKSKKIAEQTQRM
                     NARLQELLQESHETGKMLVRRDLELTQSNERLRDLDEIKSQFVSVAAHQLRTPLTGIK
                     WTLYALLEERMGKLNREQKKFAKDAYESNLRLIDLVNDLLDASRLEEGRFGFKKKRQA
                     FAPVVEETCETFRKTAAEKGIAFSLQMPKGAVPQLDFDENRMRLVLENLLDNAIKYTP
                     PGGSVTVKLWQEKSNVSVEVADMGIGMPEDQISKVFTKFFRAENAQLAQTSGTGLGLY
                     LVKNIVEHHGGAVFFTTKENKGSAFTFTLPTPQSDTKKI"
     gene            6863..7132
                     /locus_tag="VFE94_03535"
     CDS             6863..7132
                     /locus_tag="VFE94_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZX50186.1"
                     /translation="MKKTITLHPSRIGCPSIPGTMKGIVMSIPGVEDVKVRYEERSLD
                     ITFDEAKTTPEAIMEKIGQEMGLAMEVADSHGAKKEGNVSETCPM"
     gene            7251..8288
                     /locus_tag="VFE94_03540"
     CDS             7251..8288
                     /locus_tag="VFE94_03540"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZX50187.1"
                     /translation="MASPTNYYPELQEEIEKAGLMDKQPAYYYFKIFSTLALWGISIT
                     ILVVVDTFWLQLLNAAFLAFVFGQIGYIGHDAGHRGAFRSIRGNELAGLGASFFICLS
                     RSWWVTQHNQHHATPNDLEQDPHTTLPFLAFSKESALGKSKFMRRLVGYQAFYFIPLL
                     PLEGLGIRLASIQFLLKQRKVKYPVLEPLLMGLHFLLYFGLLFWFLTPWQVLGFFLVH
                     QALFGLYYGTVFAPNHKGMLVLEKDNKLDFLHTQVLTTRNVKPGLITDFWYGGLNYQI
                     EHHLFPLMPRNRLGAARKIIKTFCEQHDISYCETGTFRSYREILSYLHEVTAPVRGWP
                     WRGKGTLAQST"
     gene            8422..8868
                     /gene="sodN"
                     /locus_tag="VFE94_03545"
     CDS             8422..8868
                     /gene="sodN"
                     /locus_tag="VFE94_03545"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015226941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HZX50188.1"
                     /translation="MLYRIFEILHKIKPAKKVYAHCDIPCGIYTPEPALTAAKTVAKM
                     VEKIEELPQDMLPTLDTRNSFVRMVHVKEEHAQICKKELLILWTDYFKPEHLGDFPEL
                     HDTFWKAAKLCSKNKQGVNLQTAQELLKAVEEIAEIFRQAESSKHR"
BASE COUNT         2441 a         1932 c         2480 g         2173 t
ORIGIN      
        1 gaagctaacg cgttaagtat cccgcctggg aagtacggcc gcaaggctaa aactcaaagg
       61 aatagacggg ggcccacaca agcggtggat catgcggctt aattcgtcaa caaccgagga
      121 accttaccag ggctggaaac attcgtgaaa gcctctggaa acagaggccc tccgcaagga
      181 cacgagttca ggtggtgcac ggctgtcgtc agcatgtacc ttgaggcgct ccgttaaatc
      241 gggcaacatg cgcaaccctt accctgtgtt ttacgtgtca cagggaactg cctgagacaa
      301 tcaggaggaa ggcgaggatg acgccaagtc agcgtgtccc tttgatgtcc tgggccgcac
      361 gcatgataca atggggtaga cagcgggtcg caaagcagca atgcggagct aatccctaaa
      421 ctatccctca gttcggattg aggtctgaaa ctcgacctca tgaagccgga atcgctagta
      481 atcgcgtatc agctatggcg cgatgaatat gttcctgggc cttgtactca ccgcccgtca
      541 agtcaagcga gctgggaatg cccaaagtcc gcctatggcg gattaaggca ggctcagtga
      601 gagggactaa gtcgtaacaa ggcacgggta gcggaagctg tccgtggatc actacattct
      661 acccagtttg actggggagt gtcgaccaga gtcggttcgt gagatatctc gaactgaatc
      721 tggggtgttg gtcgccaacc tttgtatggc gactgggtcg gggcaactca aagaaataca
      781 acgttggttt cagaaaacaa actcttgcag aagggtttgt tttcgtataa tatagtcaat
      841 ggtacctttc attatacttg aagggtgaga atatatttgt tctcgcccgt tcgcgcgtgt
      901 agttcattgg cagaacgctt ctctgataaa gaagaggcag aaggttcgat tccttccacg
      961 cgcacccttc gaatatttaa gaaaggcttt ggagagtaaa agactatatg tctatggaga
     1021 agtttcctga acatgaacct tctgttgaag gttctagcga tgaaaaagcc tttcaggctt
     1081 atgtggaaga cttggacttg tcgcccgaag attttaacaa gaaaattctt gatgtaggga
     1141 gcggttcgag ccagttcgcc aaatgggcta aagaccacca agtgagttct gaaatttaca
     1201 gcatcgaacc atatcaacag ccccaagaaa tatccaaaag cctacaaggc cgggcagaaa
     1261 ccatgccctt ccaggataac gcgtttgatc ttgtagtatc gaatgcgtct atcccgaatg
     1321 tatttttagg agaagcccct gaagttatga aagaaaaggt tagagatagc ttggttgggt
     1381 ttatgcgtgt ggtaaggcct gggggagaaa tccgtttggg gcgagtgtta aagggaaacg
     1441 tctatgaatc gcagcaaaat atttccgctg ccgtcgagga agtcctcgaa gagttacgcc
     1501 aagcctatgg ggcagaagtc gaagaaattc gttattcggc aggggatacg tatgagtatg
     1561 acgcaaagca ggaacccata agaatccttg caagagcgta tcttataaag attcgtaaac
     1621 caaattaagc gtccttttct cgaggacgtc tctttattat ggccttgtca taaagctttt
     1681 tttctgttat cctatagatt gtttcgggcc cttagcttag tggtagagcg cttcatttgc
     1741 aatgaagagg tcaccggttc gaatccggta gggtccacat cagttattct tgttttcttg
     1801 catctatgtg gtataaatta accataagac taatcattta ttggcaggaa aattatgttt
     1861 aacccagaaa gagaacctaa cactggcgaa gaacaggaag gagggtcgga ttatggtgag
     1921 agttacgaac agggaattcg aaggaacgaa gaaggcatac acagcacgcc cgaaggtgaa
     1981 gaaattctca aagggatggc tcgcaaggca agaggggaag aacctcaaga taatcggcac
     2041 gatcaagggc aagaagagga ggtagtaaaa attactaatg aggatatatg gagaaataaa
     2101 atagaaagaa gagctgattt aattgacctg acccgtccca tacctatctc agaagaagat
     2161 ccaactagct ttcctccggg cattcttaga gatttaagag aagaaatttt taggttgagg
     2221 gcggaaggaa aaggaggcat ggatatatac agagagctgt caaagtttac ctatcctgac
     2281 ttgtaccgtg gctatgacac aaaagtacaa gaagtagcta agctttacaa tcaagcatta
     2341 aacagtttag ttaggtctaa agaggtggat acaacagagt aagaattcac ccgagcccga
     2401 agctagtttg agctaaaagc aagagaaatc aacccttcga cttcgctcag ggtaaactct
     2461 ttgccgactt tgcacggaag ccggatttta gtagaatagg ggaaagggta ttttagttca
     2521 tttggaggtt cctgccatga gtcaaaggac taggtgttat attatcgggg gaggcgccgg
     2581 tttaatgatt cttgtggcga ttgctacctt tatcgtcggc gatatggttt ttggcggtaa
     2641 gaccacgttc tacggttggt taacttgggc aacaagttct tgggttttag tttttctact
     2701 gatcggtacg agcctagtcc atatcattct tacggtgtgg gcatgggcac gccggaaaag
     2761 aatagctaac tcgtctccgt ggcaatatcg caaataaaag cattagggca ccttaccaaa
     2821 ggtgcccatt ttcttgccag ctttttaccg aggcggggtg ttggggagtg gactttttgg
     2881 tgtttcttgg tacggtgaga gtagaatttc ggccgggggt actgaaatga gaattacgat
     2941 tggccaggca gataaggctc ggggtagtct actaaagctg ctcgttcaaa aaatagatat
     3001 tgtcgagtac gtcgctatga cgcgcattga aagggttaaa acgtatgata gcggcgctcc
     3061 ttgcccgcaa gatagctgca tcgcggtaag attgtgcaag ccgttaccag aggggttagt
     3121 ttttcctcct gaagttgacg gggtgcaggt gtttgttgaa aactaaaacc ccatactgcc
     3181 gtaggccgag tttaaggggc agccgcaggc caatgcattt gctccttttt tagcgaggcg
     3241 gcgcagccag gcgcagtatt gtgcctgggt tttgttttga ctgcatacgt agtacaataa
     3301 agaataatgc gaattcgata cgaagttgta gcgttgcgga ccgggcttgc ttttgtgttt
     3361 ttgtacacgg cggtcgttgg gtttatggat cctggcgcgt atatcaagtg gattccgggt
     3421 tttgtgcttg agtcgcttcc ggttggcgcc gtgccgttcc ttatttcgtt tttcattttt
     3481 gaagccgtac ttgcggtttg gctgttatgg ggaaaatggg gcgtggccgc ggcagttata
     3541 agcgccgcgc tgttgtttgc aataacattt ttaaatctta acgcgtttgg cgttctgttc
     3601 aggaatgtcg gccttatcgc ggcagccgta gcgcttggtt ttatggaagg aaaaggtcaa
     3661 acaagaaatc gataaatgaa gtaagataca tggaaaagca ttatatatgc accggtggat
     3721 gcggaggaat ggcagaaact cccggcgttt gccaaactga aggttgccca gactatggcg
     3781 aacctctgaa agaatgcaat tgtaccgacg gccggcaccg tgaggaaaac tcggcctctg
     3841 gacgggaaaa ccagaaaact ccataatata gtaatgaccg gaaagaaaaa atacattctt
     3901 cttattgaag acgaagccac tctctcaaac cttatagagt ctggattgcg agaccaaggg
     3961 tatgaagtaa aggcggcaaa agacggaaac gaaggactgc ggcttattcg ggaaacaaag
     4021 ccggatttgg ttctgctcga tattatgctg cccggactca agggatttga cattttagaa
     4081 aaactgcata aagaagacgg cgtgctgccc ggcttgcccg tgattatcat ttccaactcc
     4141 ggagattcca ttgagatcga acgcgcgctc cacatgggcg tgcgagatta tctggtaaaa
     4201 gtgaatttta accccaacga ggttgtggaa aaagtgaaca gtgtgctcga ggcggaagcc
     4261 gacaagaaaa agaaaaagcc cgtcaaactc cagcccgtga aggggaatcg ggttctcctg
     4321 gtggaagacg atacaattct ttctgacgct ttggagagaa agtttgttga gaaaaagtat
     4381 acggtgctca aggcgctcaa cgcggccttg gcgcgaaggg ttttggaaga acatgaagta
     4441 gacgttattt tgcttgacct tgttttgccc gatgagcacg gtttgagctt tttgcgccag
     4501 ctgaaaaaaa acgacgctac aaaagacatt ccggtcatta ttacttcgaa tctaggccag
     4561 caggaagaaa ttgacgaagg gctaaaagcc ggcgcggttg attacatcgt aaagacgaat
     4621 accgttcccg gcgagatctt tgaaaaagtt caagcattag tcaagaaagg tcaagattaa
     4681 taacattcta gcgttattat tgcatggcaa tttacgcggg aataggttta agcaaagaga
     4741 aggacgcctt ccaggcaggg tacgcggcct gtaaaaccgc gatggaggcg gcgggcggca
     4801 aaacgcccga gctgaccatt gtattcgctt ccgtgagttt tggccagcca gaggtcttaa
     4861 agggcgtgcg cgaagcaagc ggcggcgctc ccttggtagg atgctcggac gcgggagaga
     4921 ttaccaacga aggcccgagc cagcactcgg tcggggtaat ggcaatttcg tcagaccaaa
     4981 ttgattttta cacgggtctg ggaagagacg tgaaaaaagg agcgagagag gcgggccagg
     5041 cagtggccaa agaagtaaaa gaaaaggcca aggacccctt acaagcgttt atcatgtttc
     5101 ccgacgtctt aaccggaaac ggggcagaca tcgtgcgcgg agcgctcgac gtgctgggcg
     5161 agcattttcc ggttgtgggc ggagccgcgg gcgacgactt tctgtttgag aagacgtatg
     5221 aataccagga cgaggaagct gcttggggag cggtaggagg ggtgggtctc gcagggaaat
     5281 tttccatggg agccggggtt cggcacggat gggtgcccat cggcgtgccc atgaaagtga
     5341 cgaaagcggc agggtcggtg gtgcatgagt taaatggccg tcccgcagta agcatttacg
     5401 aggactactt tggcgataaa gcgggagagc tgcgtaaaga acctttggcg agaatggcga
     5461 ttacctatcc tttgggaatt aagcttccgg gcatggatga gtatcttatc cgcgatccca
     5521 ttacggttga tgaacaagga gctatcacgt gtgctgccga aattccggag ggttctgaaa
     5581 ttcggctcat gatagggagt accgaaaagg ccgtggaggc ggctcaagac gccgcgcgca
     5641 agctcatggc cgattttgaa aaacagggct cgcaaccaaa gtttgtgctc atgtttaact
     5701 gtatcgcgcg ggaaaaactg tttgggcagc gggcgaatga agagatccag gcggtgatgg
     5761 aaattatcgg taaagacgta cctctgctgg ggttttatac ctatggggaa caggcgccta
     5821 tcggcgggga actgcacgac acggaaaaaa tcagctcgcg tttttacaac gaaaccgtgg
     5881 tgatgtttgc cgttggcgag taacggctca aagaacccat ggatatattt ggcgtacaaa
     5941 ccttggtatt cgggtggttg cttttgattg tgagcggcgc ggtgttggcg agtcgcgcgg
     6001 tcgcggtgta tcggaaatcg aaaaaaattg cggagcaaac ccagcgaatg aatgctcgcc
     6061 tgcaggaatt gttgcaagag agccatgaaa caggcaaaat gctcgtgcgg cgcgacttgg
     6121 aacttacgca gagcaatgaa cgcctgcgcg atttagacga gataaagtcg caattcgtgt
     6181 cggtggccgc ccaccaactg cgcacgccct taaccggcat taagtggacc ttgtacgccc
     6241 tgctggaaga acgcatggga aagctgaaca gagaacagaa aaagttcgcg aaagacgcgt
     6301 acgagtcaaa cctgcgtctg attgatttgg tgaatgattt gctggacgcc tctcgtttgg
     6361 aagaaggaag gttcgggttc aaaaagaaaa ggcaggcgtt cgcgcccgtg gtggaagaaa
     6421 cctgtgaaac cttcagaaaa accgccgccg agaaaggcat cgcgttctct ctgcagatgc
     6481 caaaaggcgc cgttccccag cttgattttg acgagaaccg aatgcgcctc gtgctggaaa
     6541 atctgctgga caacgcgatt aaatacacgc ctccgggggg ttctgtgacc gtgaagctct
     6601 ggcaggaaaa gagcaatgta agcgtggaag tggcagatat gggaatcgga atgcccgaag
     6661 accagatttc caaagtgttc accaagtttt tccgggccga gaacgcccag cttgcgcaga
     6721 caagcggcac gggcctgggg ctttatttgg taaaaaacat cgtagagcat cacggaggcg
     6781 ccgtattttt cacgacaaaa gaaaacaagg gaagcgcgtt tacgttcacg cttcccactc
     6841 cacaatctga tacaaaaaaa atatgaaaaa aaccattaca ttgcatccat cacgaatcgg
     6901 ctgcccttct atccccggaa ccatgaaggg aattgttatg tcaattcccg gcgttgagga
     6961 tgtcaaggtt cgctacgaag agcgctccct cgacataacg ttcgacgaag ccaaaaccac
     7021 gcccgaagct attatggaaa aaatcgggca agaaatgggc ttggcaatgg aagttgccga
     7081 ttcccacggc gccaagaaag aaggaaatgt ttctgaaacc tgtccgatgt gaatggaatt
     7141 gaattttttt aacgaggcgc gcagaatacc ttacgcgcct tttgcttttg aggtaattgt
     7201 ggtatggtat aacgagattc gcaagctgat ctttagaaag gagaaaattc gtggcatcgc
     7261 cgactaacta ctaccccgag ctccaggaag aaattgagaa agctggattg atggataaac
     7321 agccagctta ctactatttc aaaatctttt cgaccctggc attatggggt atcagcatca
     7381 ccattctggt ggttgtagat accttttggc tccagttgtt gaacgctgca ttcttggctt
     7441 tcgtgttcgg gcaaattggg tatattggtc atgatgccgg acaccgcggc gcgttccgat
     7501 cgattcgggg aaacgagctt gcaggtcttg gtgcaagctt ctttatttgc ctaagccgaa
     7561 gttggtgggt tacccagcat aaccagcatc atgcgacccc caacgatctt gagcaggacc
     7621 cgcataccac gctgcccttc ctggctttct ccaaggagtc ggcgcttggc aaatcaaagt
     7681 tcatgcggcg cttggtgggg tatcaggcat tctattttat acccttgctg cccttggaag
     7741 gcctgggtat tcgcctggcc agcatccagt ttttgctgaa acagcggaaa gtaaagtatc
     7801 ctgtcctcga gcccctgctt atggggttgc acttcctgtt gtactttggc ctgctgtttt
     7861 ggtttttgac tccctggcag gtattgggtt ttttcctagt acaccaagcg cttttcggcc
     7921 tgtactacgg tacagtcttt gcacccaacc acaaagggat gttggtcttg gagaaagaca
     7981 acaagcttga tttcctgcac acccaggtgc tcactacccg taatgttaaa ccgggcctta
     8041 ttaccgattt ctggtacggc ggcctgaact atcagatcga gcaccacttg ttccctttga
     8101 tgccgcgcaa caggttggga gcagccagaa agattatcaa gaccttttgc gagcagcacg
     8161 acatttctta ctgcgagacc ggcacgtttc ggtcgtatcg ggaaattctt tcctatttgc
     8221 atgaggttac tgcccctgtg aggggatggc cttggcgagg gaaaggaact ctcgcgcaaa
     8281 gcacctaata cgtaagacca tatcatccag cccctgtgta tcgcacgagg ggctttctat
     8341 tgacgaaggg ttatggttga gtacactgga ggtaggggag agatataaca aaaaagctcc
     8401 tctaacgtat tacatataaa tatgctttac cgtatctttg agattttgca caaaataaag
     8461 ccggccaaaa aagtgtatgc ccactgcgac attccctgcg ggatttatac gccagaaccc
     8521 gccttgactg cggcaaaaac cgtggcaaaa atggtggaga aaatcgagga gcttccccag
     8581 gacatgctcc caactctcga tacgcgaaat tcctttgtgc gcatggtgca cgtgaaagaa
     8641 gagcacgcgc aaatttgcaa gaaagagttg cttatccttt ggacagatta ttttaagcca
     8701 gaacacctgg gggattttcc cgaacttcac gatacatttt ggaaagcggc aaaactttgt
     8761 tccaagaaca aacagggagt gaatctgcaa actgcacaag aattattgaa ggcggtagag
     8821 gaaattgcag agatatttcg ccaggccgaa agttcgaaac accgatgatt ccttatttta
     8881 ttgtaaaagg ccaaagtatg gagcccttgt gcaaagaagg ggattttgtc ttgctcgaca
     8941 agctttcgta tattatgcta aagccaaagg tgggggatat gattgtgttg cgccatcccc
     9001 aggaagaggg ccgtttgatt ttaaag
//
LOCUS       DASRUP010000087         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1182536, whole genome shotgun sequence.
ACCESSION   DASRUP010000087 DASRUP010000000
VERSION     DASRUP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182536"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFE94_03550"
     CDS             complement(<1..732)
                     /locus_tag="VFE94_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HZX50189.1"
                     /translation="MKNTYIIGGLILLLAIAGGAVYLVTQNQQAEDTTQQTQNGGVTP
                     QPQPSPTPEPSQENEPQSVLGKSVEGRDITAYHFGSGDKELLFVGGMHGGYSWNTALV
                     AYELMDYLEANPNGIPANVKVTVIPVLNPDGLEKTVGTAERFTKANVPVSLEATIPGR
                     FNANNVDLNRNFDCDWQSVGIWQSREVDGGASAFSEPESRAFRGYVQANNPAAVLVWF
                     SAAGGVYASHCGNDPILPETTAITNA"
     gene            complement(976..1263)
                     /locus_tag="VFE94_03555"
     CDS             complement(976..1263)
                     /locus_tag="VFE94_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50190.1"
                     /translation="MSIVAEVLSIFERLLEGEKYEEVRKLEDEQGLYLWDIKISGEEG
                     GIEYSYMRQGQYPEGQALATAVHVAFFDSEGMPIGGHSVAKYVDGQWQLTP"
     gene            complement(1321..1611)
                     /locus_tag="VFE94_03560"
     CDS             complement(1321..1611)
                     /locus_tag="VFE94_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50191.1"
                     /translation="MRDSKPWRGKAFDMLHLTTIIKVMKKKNITMDDLAGMAKREFDE
                     IHKKLGKLDTLEKNDQVILKRLEGVVYRTEFERLEIRVKELEDLLAINGKKS"
     gene            complement(1624..1764)
                     /locus_tag="VFE94_03565"
     CDS             complement(1624..1764)
                     /locus_tag="VFE94_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50192.1"
                     /translation="MAKRRSGVHNKTTHSRKGPKRLAVKKRMLEIRAMKPAERRKALR
                     GE"
     gene            complement(1813..>2484)
                     /locus_tag="VFE94_03570"
     CDS             complement(1813..>2484)
                     /locus_tag="VFE94_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX50193.1"
                     /translation="RDAAKEAEESKVEVRKEDKTIRPFTIPTQDNMGGEILRITSFSV
                     MKGLKAVQKNTNISLRKNEHLLLLGPNGIGKSTLLESLASGKAKGATIAPHMEVGYYR
                     QDFSTLNFADTVYDSLAQVSKDRSEQEVRSVAASFLITKELIYAKVGSLSEGQKGLVS
                     FARLVLQKPGLLILDEPTNHINFRHVPVIASALNKYEGAMVLVSHVPEFVGQIRIDQV
                     LDLEK"
BASE COUNT          569 a          667 c          524 g          724 t
ORIGIN      
        1 tgcattggta atagccgtgg tttctggcaa aatggggtcg tttccgcaat gcgaagcgta
       61 tacgccgcct gccgcgctaa accacaccaa tacagcggct gggttgtttg cctgcacgta
      121 acctcgaaac gcccggcttt ccggttcaga aaacgcgctt gctcccccgt ccacttcccg
      181 gctttgccaa atgccaaccg actgccagtc gcagtcaaag ttgcgattta agtccacatt
      241 gtttgcgtta aaccggccgg gtattgttgc ctccaaagag actggtacgt ttgctttcgt
      301 aaaacgctcc gcagtgccga cagtcttctc caaaccgtcc gggtttaaca ccggaattac
      361 ggttactttt acgtttgccg gaataccatt ggggtttgcc tccaaataat ccataagctc
      421 ataagcaacg agcgccgtgt tccatgaata cccgccgtgc atgccgccca caaacaaaag
      481 ctccttatcg cccgagccaa agtggtacgc ggtaatgtcg cgcccttcca ccgattttcc
      541 caaaacagat tggggctcgt tttcctgcga aggttccggc gttggacttg gttgcggctg
      601 gggcgtaacc ccgccgtttt gcgtttgttg tgttgtatct tctgcctgct ggttttgcgt
      661 tacgaggtac acggcgcccc ccgcaatcgc cagcaacaga ataagccccc ctataatgta
      721 agtgtttttc atattgttat tgtaacgtaa aagcccgaaa aatgcgactg cggctttagc
      781 ttgggaaccg cagcaatata cccttgtacg cttttggcct ttacggcctt acacatgttc
      841 atgccgccat tgtagcactt ggacaaactc agtgtaaaca aaaaactgtc ctgagctttg
      901 tcgaaggacg atgaaagatt cgtacaaaat taaaggcggt catgctatgg gtcgccttta
      961 tacggtcaac taaaattatg gggttagctg ccactggccg tcgacatact tcgccactga
     1021 atgaccacca attggcatgc cttcgctgtc aaaaaacgca acatgaacag ctgtcgctaa
     1081 agcttgtcct tcaggatatt gcccctgtct catgtaagaa tactctatgc ctccttcttc
     1141 tcccgaaatt ttaatgtccc ataagtaaag accttgttca tcttcgagtt tccgtacttc
     1201 ttcgtatttt tccccctcta gtaatcgctc aaatattgaa agtacttccg ctactatact
     1261 caaatctttt ttttaacgct gctcccttag tgcacaaagt cagaacctgt tttacaagca
     1321 ttagcttttc ttgccgttga ttgcaaggag atcttccaat tcttttaccc ttatctcaag
     1381 cctctcaaat tctgtgcggt aaacaacacc ctccaatcgt ttcaggatca cttggtcgtt
     1441 tttttccaaa gtgtccagtt taccaagctt cttatgaatc tcgtcaaatt ctctcttcgc
     1501 catccccgca aggtcatcca tggtgatgtt tttctttttc atcaccttta ttatagtcgt
     1561 aaggtgcaac atgtcaaacg ctttgcccct ccacggcttg gagtctctca aaactcagta
     1621 agattattct ccccgaagag cctttctgcg ctcggcgggt ttcattgctc ttatctcgag
     1681 catccgtttc tttaccgcga gccgttttgg gccttttctt gaatgagttg ttttgttgtg
     1741 tactcctgat cttcgttttg ccatagtata cccagcatta cacacaaacc gacgctttgc
     1801 aactaagact gactacttct ccaaatcgag aacctggtca atgcggattt ggccaacaaa
     1861 ttcgggaacg tggcttacca acaccatggc gccctcgtac ttgtttaagg ccgaggcaat
     1921 aacgggcaca tgccgaaagt ttatatggtt ggtgggctcg tctaaaataa ggagtcccgg
     1981 cttttgcagc accagcctgg caaaagaaac cagccccttt tggccttcag acaagctgcc
     2041 taccttggcg tatattaatt ccttggttat taaaaagctg gctgccacgg aacgcacctc
     2101 ttgctctgac ctgtctttgg aaacttgggc aagcgaatcg tatacggtat ctgcgaaatt
     2161 aagcgtggaa aaatcctgcc ggtaataccc cacttccata tggggcgcta tggttgcgcc
     2221 ttttgccttg ccgcttgcca aagactcaag caacgtgctt ttgccaatgc cgtttggccc
     2281 caacaacaac aaatgctcat ttttccgcaa agaaatattt gtgttctttt gtacggcctt
     2341 gagccccttc ataaccgaaa aagaggttat gcgcaaaatt tccccgccca tattgtcttg
     2401 cgtaggtatg gtaaaggggc ggatggtttt atcttctttg cgcacctcta cttttgactc
     2461 ttctgcttct tttgcggcgt cccg
//
LOCUS       DASRUP010000088         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2308455, whole genome shotgun sequence.
ACCESSION   DASRUP010000088 DASRUP010000000
VERSION     DASRUP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308455"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1362)
                     /locus_tag="VFE94_03575"
     CDS             complement(193..1362)
                     /locus_tag="VFE94_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX50194.1"
                     /translation="MRTVSKPIILLGIALFFIFLGFNATQQFVTPFFEQRGIVEVGFQ
                     SLILIYVFFVFTNPFAATIAVKYGPKVLVAGGSLAYSLYIASLASGSLPFIYAASAVL
                     GVGAGFFWMGENSYLFRTSKEDERGKNVGFIDTLLYLGTASGVLGVGFLVSSVSFRWM
                     FLFLAFMPLIGFLVLLRLPRLHIEQSGENRFVLLRKAVQSTAAIRIAGIWLAMFMVMG
                     LVIGILPVQIAKKFDLVYVGVLTSLPFLLPVFLSYGLGRISDRVGRKKVIAVAYALVV
                     AGMVVLQFSDIPVLLVIGSVLLALAWTLTRIGILPLIGDIGSGKNLEALIALFWMSQN
                     AGVILALLLGRFLNENIGFLYGIALAVVLLSFAVAFPLFRTSIGDVRKRLGEEIA"
     gene            complement(1366..>2138)
                     /locus_tag="VFE94_03580"
     CDS             complement(1366..>2138)
                     /locus_tag="VFE94_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZX50195.1"
                     /translation="LAKGVESLGQKSVELSDTLYAAEQEIEGTQQNVEAVQSRLGGFE
                     QQVGQISGTVNTLEKLSKTDPELLQKYSKVYFLNEHYVPERLTQIPSNYLYSDNSPEH
                     IHTVVWPYLKELLDAAQLANVPVYIRSAYRSFDEQSALKTQYTVLYGAGTANQFSADQ
                     GYSEHQLGTTVDFITTGLSGQLTGFENTQAYQWLLANAHKYGFTLSYPSGNPSYIFEP
                     WHWRYVGISLAGYLHDQNKYFYDFEQREIDEYLVNLFD"
BASE COUNT          632 a          553 c          443 g          510 t
ORIGIN      
        1 cgagagatga atccaaatcc gcggtctgtg agtcgaacta tggttccttg ttccatttta
       61 aaagaaacta gctcgttacc ctatcggacg cagctggctc cagactaaaa cccgacttcg
      121 tttttcttgg agttctggcc cgatgagatt aatgctacca caccttatca cctcaagtca
      181 agtaggcttg agctaggcaa tttcttcgcc caggcgtttt cgtacatccc ctatgcttgt
      241 ccggaacaag gggaaagcca ctgcaaaaga gagtaatacg acggcaagcg caatgccgta
      301 caaaaagcct atattctcgt ttaagaatct tcccagaagc agggccaaaa tcacccccgc
      361 gttctgggac atccagaaaa gcgcgatcag ggcctcaaga ttttttccgc tgccaatatc
      421 gccaataaga ggaagaattc caatgcgggt taaggtccac gccaacgcca gcaaaactga
      481 ccctatcacc aaaagcacag ggatatcgga aaactgcaaa acaaccatcc cagcgactac
      541 caaagcgtac gctactgcaa ttactttttt tcttcccacg cgatccgata tccttccgag
      601 tccatacgac aaaaaaacag ggagtaggaa aggaagcgac gtaagcactc caacatagac
      661 taaatcaaat ttctttgcga tttgcacagg aagaattcca attaccaaac ccataaccat
      721 aaacattgcc agccatattc ccgcaattcg tatggcagcg gtgctttgca cagctttccg
      781 cagcagcacg aaacggtttt ctccgctctg ctctatgtgc aaacgaggca accttaacaa
      841 caccaaaaac ccaatcagcg gcataaaagc aagaaacaaa aacatccacc ggaaactcac
      901 agaggaaacc aaaaaaccca cccccaacac cccgctagca gtccctaagt aaagaagcgt
      961 gtcgataaac ccgacatttt taccgcgctc gtcttctttg cttgttcgga acaagtagct
     1021 gttttccccc atccaaaaaa accctgctcc cacccccaat accgcagagg ctgcatatat
     1081 aaaagggaga cttccggaag ccaggcttgc aatatacaag ctatacgcca gagaaccccc
     1141 ggctaccaga actttagggc catactttac ggctatagtt gccgcaaaag gattggtaaa
     1201 aacaaaaaat acataaataa gaattaacga ctgaaatcct acctctacaa ttccccgctg
     1261 ctcaaaaaac ggagtaacaa actgctgtgt tgcgttaaac ccgagaaaaa taaaaaacag
     1321 ggcaatgccc aaaagaataa tcggtttgga aacagtacgc atgttttaat caaacaggtt
     1381 taccaagtat tcgtctattt cgcgctgctc gaaatcgtaa aaatatttgt tttggtcgtg
     1441 aagatatccg gcaagactta tacctacata gcgccaatgc cacggttcaa aaatatacga
     1501 agggtttccc gagggataag aaagcgtaaa gccgtatttg tgtgcgttcg ccagcagcca
     1561 ctggtaagcc tgcgtgtttt caaatcctgt aagttggccc gaaagccccg tagtaataaa
     1621 atctacggtt gtacccagct ggtgttcaga atacccctgg tcggccgaga actgatttgc
     1681 ggtgcccgcc ccatacaaaa ccgtgtattg ggtttttaag gccgactgct cgtcgaacga
     1741 gcggtaggcg gatcttatat acactggcac atttgctaat tgagccgcat ccaaaagttc
     1801 tttgaggtaa ggccatacca ccgtgtgtat atgctctggc gaattgtcgc tatacaggta
     1861 gtttgaagga atttgcgtta agcgttctgg cacgtagtgc tcgtttaaga aatatacttt
     1921 tgaatatttt tgcaaaagct ctgggtcggt tttgctaagc ttttccaaag tattcacggt
     1981 gcctgaaatt tggcctacct gctgttcaaa accccccagg cgactttgta ccgcttctac
     2041 gttttgttgg gtgccttcga tttcctgctc tgcagcgtag agggtgtcgg aaagctccac
     2101 tgatttctgc cctaaactct cgactccttt cgcaagca
//
LOCUS       DASRUP010000089         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2153778, whole genome shotgun sequence.
ACCESSION   DASRUP010000089 DASRUP010000000
VERSION     DASRUP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2153778"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /gene="leuS"
                     /locus_tag="VFE94_03585"
     CDS             complement(<1..1855)
                     /gene="leuS"
                     /locus_tag="VFE94_03585"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZX50196.1"
                     /translation="MAKYDHQKIEKKWQREWSKQEEFRAQEKSSKQKLYVLDMFPYPS
                     GSGLHVGHVEGYTATDIYSRAKRMQGYNVLHPMGWDAFGLPAENYAIKTGVHPKKTTE
                     TAIKTFRKQMDRMGFSYDWSREIGTHTPEYYKWTQWFFLLLYNNGLAYKAKASVNWCP
                     SCQTVLANEQVVNGKCERCSTQVIQKELEQWFFKITDFAEDLIADLDKVDWPESTKLN
                     QKNWIGRSQGAELNFPILNSQFSINVFTTRPDTLFGATYMVLAPEYTLVDGLKEQITN
                     WQEVEKYREEAKKKNEAERTELAKEKTGVELQGVKAINPGTKEEIPVWIADYVLASYG
                     TGAIMAVPAHDERDFEFAQKYNLPIVMVVCPHYPAKTCPVMDEAYLGEGHLVASGKFD
                     GMPNEKAKWEITKAVGGKRKTNYHLRDWLVSRQRYWGAPIPIVYDPEGKPHAVPEKHL
                     PWFLPTDVEFKPTGYSPLADSKEFVKRTEKIFGKDWKPEVDTMDTFVCSSWYYFRFAD
                     PTNKKEFASARALKQWLPVDMYVGGAEHTVLHLMYARFFTKVLKKLGYVEFGEPFQKL
                     RHQGIILAENGQKMSKSKGNVINPDDIIEEYGADPLRMFEMFLGPLEDMKPW"
     gene            complement(1879..2487)
                     /locus_tag="VFE94_03590"
     CDS             complement(1879..2487)
                     /locus_tag="VFE94_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HZX50197.1"
                     /translation="MAYVPHDKFAQKAKEKGFRARSAFKLLNLQNKFQLLKKGDMVLD
                     LGAAPGSWLQVVSQIVGNEGFVVGVDITPIENLGLPNVKTFQKSIADQDFVEFLRKRD
                     PAIFNVVLSDIAPNTTGIKARDEALSHELSMRVLEIAMQVLRKKGNLAIKVFEGPETP
                     ELLKEAKKHFARVHLAKPMASTKGSKELFLVAQNFLQNGQMA"
     gene            2520..>3304
                     /locus_tag="VFE94_03595"
     CDS             2520..>3304
                     /locus_tag="VFE94_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50198.1"
                     /translation="MTIAKFQKTILNWYAENRRDMPWRETRDPYKILVSEIMLQQTQI
                     PRVLPKYQEFLQEFPTPQALAKAPDKKLLKVWAGLGYWRRAKYLKKAARTVDLRRRVG
                     PRAKRGVKRDEKLQSERNFLETLPGVGHYTARAVACFAFGSKEAFLDTNIRRVYLHFF
                     FGDKNGVTDKEILQIAQKAIDTLPKGTTPREWHYALFDYGAVVLKKDRTINKRSRHYA
                     KQSKFAGSFRSFRTKAVRFILASPSNKVSHKELHTFLKKELADS"
BASE COUNT          824 a          846 c          705 g          929 t
ORIGIN      
        1 tccaaggctt catgtcttcc aaggggccca aaaacatttc aaacatacgc agggggtctg
       61 ccccgtattc ttcaataatg tcgtctgggt ttatgacgtt acccttagat ttagacattt
      121 tctgcccgtt ttcagccaaa ataatgccct gatggcgcag tttttggaac ggttcgccaa
      181 actccacgta cccaagtttt ttaagcactt tcgtgaaaaa cctggcgtac attaagtgca
      241 atacggtatg ctctgccccg cccacataca tgtccacggg aagccattgt ttaagagccc
      301 ttgcagaagc aaattctttc ttgtttgtag ggtctgcaaa tcgaaaataa taccaggaag
      361 agcatacaaa agtatccata gtatctactt cgggtttcca gtctttgcca aagatttttt
      421 ccgtacgttt cacaaactct ttggagtctg ccaaaggcga gtatccggta ggtttaaact
      481 ccacgtctgt cggcaagaac caaggcaaat gcttttctgg caccgcgtgc ggcttgccct
      541 ctgggtcgta cacaatagga atgggggctc cccaatatct ctgcctcgac accagccaat
      601 ctcgcagatg ataatttgtt ttcctttttc cgcctacagc cttggtaatc tcccattttg
      661 ctttttcgtt cggcatccca tcgaacttcc ccgaagctac cagatggcct tcaccaagat
      721 acgcctcgtc catcacagga caagttttgg caggataatg cgggcatacc accattacta
      781 tgggcaagtt gtatttttgg gcaaactcaa aatcgcgctc atcatgcgcg ggaacagcca
      841 taatagcgcc tgtgccataa ctagccagca cataatctgc aatccatacg gggatttctt
      901 ctttggtgcc tggatttatt gcttttactc cttgcaattc tacgcctgtt ttctccttgg
      961 caagctcggt gcgctctgct tcattctttt tcttcgcctc ttccctatat ttttctacct
     1021 cttgccaatt tgtaatttgc tcttttagcc catcgaccaa cgtatactct ggagctaaaa
     1081 ccatataggt ggcaccaaaa agcgtatcag gccgcgttgt aaaaacgtta attgaaaatt
     1141 gagaattgag aattggaaaa tttaattcag ctccttggga gcggccaatc cagttttttt
     1201 ggtttaactt ggtggattct ggccaatcta ctttgtccaa atccgcaatt aaatcttccg
     1261 caaaatcggt aattttaaaa aaccattgct ccagttcttt ttgtataact tgggtgctgc
     1321 accgttcgca ttttccgttt acgacctgct cattagccaa cacggtttgg cacgaagggc
     1381 accaatttac gcttgccttt gccttgtatg ccaagccatt attgtacagc agcaaaaaaa
     1441 accattgcgt ccatttataa tattctggcg tgtgcgtgcc aatttcgcgg ctccagtcgt
     1501 aggaaaaccc catgcggtcc atttgctttc gaaaggtctt aatggccgtt tctgtggttt
     1561 tctttgggtg tactccggtt tttatcgcgt agttttctgc gggcaagcca aaagcgtccc
     1621 accccatggg atgcagcacg ttgtagcctt gcatgcgctt ggctcgagag taaatgtcgg
     1681 tggcggtata cccttccaca tgccctacat gcaagcccga tcctgaagga tacggaaaca
     1741 tgtccagcac atacagcttt tgtttggaag atttctcctg cgcccgaaac tcctcttgtt
     1801 tactccactc cctctgccac tttttctcta ttttttggtg gtcatactta gccatatacg
     1861 gcctaattct agctattttt aggccatttg tccattctgt aaaaagtttt gggcgacgag
     1921 gaaaagttct ttgctgcctt tggtggaggc cataggcttt gccaaatgca cgcgggcaaa
     1981 gtgctttttt gcttctttga gaagttcggg ggtctctggc ccttcaaaaa cttttatcgc
     2041 aaggtttcct ttttttctta acacctgcat tgcaatctcc agcacgcgca tggagagttc
     2101 gtgcgaaagc gcctcgtccc tggcttttat gcctgtggtg ttgggcgcaa tatccgaaag
     2161 cactacgtta aagatcgcgg ggtctcgttt tcgcaaaaac tccacgaaat cctggtctgc
     2221 aatgcttttc tgaaaagtct ttacgttcgg caggcctaaa ttctctatgg gcgtgatatc
     2281 tactcccacc acaaaccctt cgttgcccac aatttgcgaa accacctgca gccagctgcc
     2341 gggagctgct cccaaatcca aaaccatatc cccctttttg agaagctgga atttattctg
     2401 gagattgagt aacttaaaag cagagcgggc cctgaaccct ttttctttcg ctttttgagc
     2461 gaatttgtcg tgcggaacat acgccatacc tatctttctt gtatgctaca atagcaatta
     2521 tgacaatagc aaagttccag aaaaccattt tgaattggta cgcagagaac cgcagagata
     2581 tgccttggcg cgagacccga gacccctaca aaatcctggt ttcagaaatt atgctgcagc
     2641 aaacacagat tccccgcgta ctgcccaaat accaggaatt tttgcaggag tttcccaccc
     2701 cacaagccct ggccaaagcc ccggacaaaa aactcttaaa agtatgggcg ggtttagggt
     2761 actggcggcg agcaaaatac ctgaaaaaag cagcgaggac tgtagatttg aggagacgcg
     2821 tagggccccg cgcgaagcgt ggggtaaagc gcgacgaaaa gctgcagtcc gagcgaaact
     2881 ttctcgaaac gctcccaggt gtaggccact acaccgccag ggccgttgcc tgctttgctt
     2941 ttggtagtaa agaagcgttt ctcgacacca atatccgcag agtatatttg catttctttt
     3001 tcggagataa aaatggtgtc acagataaag aaattttgca aatcgcgcaa aaagcaatcg
     3061 acaccttacc caaaggcaca actccaagag aatggcacta cgcgctgttt gactacggcg
     3121 ctgtggtact caaaaaagat cgcaccatta acaaacgcag ccgccattac gccaagcaaa
     3181 gcaaattcgc gggctctttt cgttctttcc gcaccaaagc cgtgcgtttt atcttagcca
     3241 gtccaagcaa caaagtttcg cataaggagc tacacacgtt tctcaaaaaa gagctggcag
     3301 attc
//
LOCUS       DASRUP010000090         6063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2323781, whole genome shotgun sequence.
ACCESSION   DASRUP010000090 DASRUP010000000
VERSION     DASRUP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323781"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFE94_03600"
     CDS             <1..571
                     /locus_tag="VFE94_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZX50199.1"
                     /translation="LQKHGVKEVVLLLHKGETEAQKILGDGAKYGTKITYLWQGEESI
                     GTAHTVSLAKKFVGEDFFFVLYCHVVADLDLMDLASYHADAGVTATLALTSIEDPSPY
                     GAVQMRGEKIVGFTEKPKEKEGVSRVISAGIFCLSPEIFEYIPKQKTASLERDVFPGL
                     AKEGKLAGYMFEGKWCNVRNRIYRSGKKN"
     gene            708..2390
                     /locus_tag="VFE94_03605"
     CDS             708..2390
                     /locus_tag="VFE94_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50200.1"
                     /translation="MQKYLFRDEGARAIDYLKLGKTGEEAVGNFFQGLCAISRRVLND
                     EVEIVSCDLTACQEWISTTANGYKTISMDSYFSGDFQVRVSRVFLPGGKRNIGIVERP
                     GFSTLQQQVARIPPGTYNLVDDMMYTGRTVRPILEQLGAQGVSIAEVLVSVRVGCPEL
                     DIPVRGMVSYEPHEIRNRGIDNPRDFLAGAHEGGLVVHYPWPLPSGTIVRTPYVFPFV
                     DPSARIGVPRERALEFSRRVWELNCVFWGQFPSVRVCDTAAAFAYGVERLGIPADEPM
                     ERFCERMLDLLNFPEIEFNVPGQGVIWLDLNDTLINIDRLLTVRKGEWMSFVQEIEGQ
                     GWMLGLCSDSPHEALKKWGERHGIHGPIIAEGGALLNGIPVSTDNPMPVEELKDLIRE
                     WADEQGIHVFPDVLSAEFQESSRALGETGIGFGAGRTQTLAVFCRRNGESDPELTKRL
                     GEFVERTKIAERFGIDPRTIDCSPSYGFFAFCSPHKKGKILRAVGWELYKQGKSCYMI
                     GNSTSDLAYCPPLCQTGIVGDGTLAAQKGADLLASAPCTQGVVELVSSIVGM"
     gene            2441..3319
                     /locus_tag="VFE94_03610"
     CDS             2441..3319
                     /locus_tag="VFE94_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZX50201.1"
                     /translation="MEVHQKTYTIVSDPGVDDMIALLLLCKLSPTLHHTLISTFGSAT
                     EEHTSQNAKEFIAFIAPHWSFFSGAKNPIKPFEHKWPDDFHGSDGIWGIHPQVSIKGV
                     VPAERFPDNSNVISLGPMTNVRTLLEEKNLREATVMGGVFNMKGNITPYAETNVAFDP
                     DAAAYLFEHCQGIDVKVVPLDVTRKVSWTKEKVTQIPENADYKIWAKKLLLAWFENYG
                     DKKQENFGLHDPLAAYSLFHPEVLEWKKVGVQVIVQGEKRGQTVLGKDNPPCQVALGI
                     PHPNDVAEDIFRLIFA"
     gene            3316..4227
                     /gene="rbsK"
                     /locus_tag="VFE94_03615"
     CDS             3316..4227
                     /gene="rbsK"
                     /locus_tag="VFE94_03615"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HZX50202.1"
                     /translation="MKNSILVAGSVNMDLVVDVEKLPAKGETVSAKTIRYIPGGKGAN
                     QAIAASRLGANVALIAKIGDDHFGEQLRSFLESEKLGLEGLAKSPLSSGLALITVGPD
                     GENTIVVLPGSNAEVTEQYIEEYAALIKETRVVISQYEIPMLSVERLFAIARKFGKTT
                     LLNPSPVKETPTELLKNVDYLVLNEVELSFLAGSAGVLEDVGDIVQAAKALQSKGPGV
                     VVVTVGARGAITVSGEKVITTEGIKVSAVDTTAAGDCFTGALAAQLQNEVPLEEALAF
                     ANRAAALSVQRWGASSSLPYLKELESI"
     gene            complement(4399..4572)
                     /locus_tag="VFE94_03620"
     CDS             complement(4399..4572)
                     /locus_tag="VFE94_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50203.1"
                     /translation="MPEKKLAPTVGEEVAKGSGLDSQAQALVKTLLDEDEAKHKWMEE
                     KFRSSDPSNSPRK"
     gene            complement(4872..5816)
                     /locus_tag="VFE94_03625"
     CDS             complement(4872..5816)
                     /locus_tag="VFE94_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZX50204.1"
                     /translation="MKKMRKFYLGVEGGATRSAAVLVDESNTVLLERIGKALNVHSEG
                     KEASKKNLISLLAPIFKKADRNKLYAVLGFASIDTKKDVALHQRLAKSVLPKNTVFRV
                     VNDAQTGIEAKCPEAKHRILVIAGTGSSVYGESEGKTAKSVGWEFPLGDEGSGFDIGL
                     KVIKAAVQSWDGRGAKTLLERLALQKGKFPQMEDFIDDLGILFHDKRQSSKYIIASFA
                     PLLNKALERNDQVGLRIRKEVVNDLTTGVSAVTNNLRIEDKDFCIGLVGSVWKMPGLQ
                     DAFKQGIERQFPRAQFSQREEGGEWGAVRLAKKLADHS"
     gene            complement(5841..>6063)
                     /locus_tag="VFE94_03630"
     CDS             complement(5841..>6063)
                     /locus_tag="VFE94_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50205.1"
                     /translation="HYAKQSKFAGSFRSFRTKAVRFILASPSNKVSHKELHTFLKKEL
                     ADSSYTPQEVTQALLKDKLIKKSKTHYSL"
BASE COUNT         1560 a         1319 c         1588 g         1596 t
ORIGIN      
        1 tttgcaaaag cacggtgtaa aagaagttgt gcttttgctt cataaaggag aaacagaagc
       61 gcagaaaatc ctgggcgacg gcgcaaaata cggaacaaaa attacgtatc tatggcaagg
      121 ggaagaaagt ataggaacgg cgcatacggt ttcgctggct aaaaagtttg tgggagagga
      181 cttttttttc gtgctgtatt gccacgtagt agccgacctc gaccttatgg atttggcttc
      241 ttatcatgcg gatgctgggg taacagccac tcttgcccta acgtctattg aagacccatc
      301 gccttacggc gcggtgcaaa tgcgaggcga aaaaattgtg ggctttaccg aaaagcccaa
      361 agaaaaagaa ggggtttcga gagtaattag tgcaggaatt ttttgcttga gccctgaaat
      421 tttcgagtat atcccaaaac agaaaacagc ctctttggaa agagatgtat tccctggcct
      481 ggcaaaagaa ggcaagctcg caggatatat gtttgaaggc aaatggtgca acgtccgcaa
      541 taggatttac cgttctggca aaaagaatta aaaatatttc ggtttggaaa ttgtttacct
      601 cttgcggggc gaataatttc cagatcaaaa acagggtctg gcagtttttt taaactgttg
      661 aacttttcac atcacaaaaa gcacaaaaga atacgaggag gattggggtg caaaaatatc
      721 ttttccgcga cgaaggagca cgggcgatcg actacttgaa actgggaaaa actggagagg
      781 aagcagttgg caatttcttt caggggctat gcgcaatatc tcgacgagtc ctcaatgacg
      841 aagttgaaat cgtgagttgc gacctgactg catgtcagga gtggatttcc acaactgcca
      901 acggatacaa gactatttcc atggattctt acttctcggg cgatttccag gttagggttt
      961 cgcgagtgtt cctgccaggg ggtaagagga acattggcat cgtagagagg ccaggattct
     1021 caacccttca gcagcaagta gcaagaattc ccccggggac gtacaatctc gtggacgata
     1081 tgatgtacac agggcggacc gtaaggccca ttctcgagca gttgggggcg cagggcgtat
     1141 cgatcgccga agttcttgtg agtgttcgcg tagggtgccc tgaactggat attccagtaa
     1201 gaggcatggt gtcttatgag ccgcatgaga tccgcaatcg gggaatcgac aacccccgag
     1261 acttccttgc gggggctcac gaggggggat tggtggtgca ctatccatgg cccctgcctt
     1321 cggggactat cgttcgcacg ccctatgtct ttcctttcgt ggacccttca gcacgcatag
     1381 gggttcctcg agagcgcgcc cttgagtttt cccggcgagt ttgggagctc aattgtgtct
     1441 tctgggggca attcccgagc gtgcgcgtgt gcgacacggc tgctgctttc gcttatggcg
     1501 tagaacgatt aggtatcccg gcggacgagc caatggaacg tttctgcgag cgcatgcttg
     1561 acttgctcaa ctttccggaa atagagttta atgttcccgg gcagggagtc atctggctcg
     1621 atctcaacga cacgctcatt aacatcgatc gtctccttac ggtaagaaag ggtgagtgga
     1681 tgagtttcgt ccaagagatt gaggggcaag ggtggatgct tgggctgtgt tctgactctc
     1741 cccatgaggc gcttaagaaa tggggcgagc ggcatgggat ccacggtccc attattgccg
     1801 agggtggggc tctcctgaac ggtattccgg tatccacaga taacccaatg cccgtagaag
     1861 agttaaaaga tctgatacgg gaatgggcag acgagcaggg aatccatgtt tttccagatg
     1921 tactaagcgc ggagttccaa gaaagtagcc gcgccttggg cgagactgga ataggtttcg
     1981 gcgcagggcg gacgcaaacc ttagctgttt tctgccgtcg aaatggcgag tctgacccgg
     2041 aattaaccaa gcgactcggt gaattcgtag agcgtacgaa gatagccgaa cgttttggca
     2101 tagatccaag aaccattgat tgttcgcctt cttatggctt ctttgccttt tgcagcccgc
     2161 acaaaaaagg caagatacta cgggctgttg gatgggaact gtataagcag ggtaagtcct
     2221 gctatatgat agggaactca acgagcgacc ttgcgtattg ccctccgctt tgccagacag
     2281 gcattgtggg ggacggaaca ttagccgctc aaaaaggggc agacctgctc gcttctgctc
     2341 cctgtacgca gggagtcgta gagctcgtaa gctccattgt gggcatgtaa agggggttaa
     2401 cgctatcgta agagatagcg ttaaccctcc tatttatttt atggaagttc accaaaaaac
     2461 atatactatc gtatctgacc ctggagtgga cgacatgatc gccctgcttt tactttgcaa
     2521 actatcgcca actcttcatc atacgcttat ttctactttc ggaagcgcca cagaagaaca
     2581 tacatcgcaa aacgcaaaag aatttatcgc gttcatcgct ccccattggt cttttttctc
     2641 gggagcaaaa aatccgataa agccatttga gcacaaatgg cctgatgact ttcacggatc
     2701 cgacgggatt tggggaatcc acccgcaagt ttcaataaag ggagttgtgc cggcggagag
     2761 atttcccgat aattccaacg tcatttcatt ggggccgatg acgaatgtgc ggactctatt
     2821 ggaagaaaag aatttgagag aggctaccgt gatgggaggc gtctttaata tgaaggggaa
     2881 tataactccc tatgcggaaa cgaacgttgc gtttgatcca gacgccgctg cttatctctt
     2941 tgaacactgc caaggaattg atgtaaaagt agttccgctc gacgtaacca gaaaggtttc
     3001 gtggacaaaa gaaaaagtga cgcaaatacc agagaatgcc gactataaaa tctgggcaaa
     3061 gaagttactt cttgcatggt ttgaaaatta tggcgataag aaacaagaaa attttggcct
     3121 ccacgatcca ctggcagcgt attccctttt tcatcccgaa gtcttagaat ggaagaaagt
     3181 tggagttcag gttatcgtcc agggggaaaa gagggggcag acggtacttg gtaaagacaa
     3241 tccgccatgc caggtagctt tgggaattcc tcatcccaat gatgttgcag aagatatttt
     3301 tcgtttgata tttgcatgaa aaactcaata ctcgtagcag gcagcgtaaa tatggatttg
     3361 gtggtggatg tagagaagct tcctgcgaag ggcgagacag tgtctgccaa aacaattcgg
     3421 tatatacccg gaggaaaagg cgcgaaccag gcaatagccg cctccaggct cggcgccaat
     3481 gtcgcattaa tcgccaaaat cggggatgac cactttggcg agcagctgcg gagtttcttg
     3541 gaatcagaaa agctagggtt agagggcctc gcaaaatctc ctttgtcgtc gggactggca
     3601 ctcattacgg taggccctga cggagaaaac accattgttg tgttgccggg aagcaatgcg
     3661 gaggtaacag agcagtatat cgaagagtac gccgctctga tcaaagagac tcgagttgtt
     3721 atttcgcaat acgaaattcc tatgctttcg gttgaacggc tttttgccat tgcgcgcaaa
     3781 ttcggtaaaa caacgttatt gaatccttct cccgtaaagg aaaccccaac agaacttctc
     3841 aaaaacgtag attatttggt gctcaacgaa gtggaattgt cttttttggc gggctctgct
     3901 ggtgttttag aggatgtggg cgatattgtg caggcggcaa aagctttaca atcaaaaggc
     3961 cccggggtag tggtggttac cgttggggca agaggcgcga taacggtatc tggcgaaaaa
     4021 gttattacaa ccgagggaat aaaagtttct gccgtagaca ctactgctgc gggcgactgt
     4081 tttactggcg cactcgctgc ccagctgcag aatgaggtgc ctcttgaaga ggcgttggca
     4141 tttgcaaacc gcgcagccgc tcttagcgtg cagcgctggg gcgcaagtag ttctctccct
     4201 tatctcaagg agcttgaaag catttagaga gagtaaaact ttttggattt ttttaggagt
     4261 ttgtctttga ggagggcttg agtaacttct tggggcgtat aagacgaatt taccagttct
     4321 tttttgagaa atgcgtgcag ctcttggtgt aaaactttgt tttttgggct ggctaaaata
     4381 aaaaagccct gcgctagatt atttacgcgg gctgttggag ggatcggaag aacgaaattt
     4441 ttcttccatc cacttgtgct ttgcttcgtc ctcgtcgagt aatgtcttga cgagagcttg
     4501 tgcctgtgag tcgagtccag accctttagc tacttcttcg ccaaccgtag gagcgagctt
     4561 tttttcgggc attttctctc cttagccgat cttccttcta aaactataga tactatacga
     4621 taagatttta tactagtcaa ggtagacaga agaggggcta aagggtatga ttggaggaaa
     4681 gatgaagaag tacgaccatc aaaaaataga gagaaagtgg cagagggagt ggaagagtgg
     4741 agaacgccta aaagcttgaa gatgccaagc gacgtttagg gtagctccac ccaaaatttc
     4801 ctttccgtct gggagtacgt caaagagtgc ttctccaaaa attatgggcc gtccattgga
     4861 ttccatttgt ttcatgaatg atcagcaagt tttttggcca aacgaactgc tccccactcg
     4921 ccgccttctt ccctttgcga gaattgcgcg cgagggaatt gtcgttcgat cccctgttta
     4981 aaggcatcct ggaggccggg cattttccat acggaaccta caagcccaat gcaaaagtcc
     5041 ttgtcttcta tacgcaggtt gtttgtaact gcgctaacgc ctgttgttaa atcgttcaca
     5101 acttcttttc gaattcgcag cccgacttga tcgttccgct caagcgcttt gttaagaagt
     5161 ggggcgaatg acgcgataat gtatttgctg ctttgccttt tgtcgtgaaa gagtatgcca
     5221 aggtcgtcga tgaaatcttc catttgagga aacttgcctt tttggagagc caatcgttca
     5281 agaagcgttt tagcgccacg accatcccag gactggacag ccgctttgat cacttttaag
     5341 ccaatatcaa agccgctccc ctcgtctccc aatgggaatt cccagccaac gctttttgcc
     5401 gttttccctt cgctttcgcc atataccgaa gatcctgtgc cggcaatgac aaggatacga
     5461 tgtttggctt cggggcattt tgcttcaatc ccggtttgtg catcgttgac cacgcggaaa
     5521 actgtattct tgggaagaac agattttgcc agtctttggt gaagggctac atctttttta
     5581 gtatcaatac tggcgaatcc taacaccgcg tacaatttgt tccgatctgc ctttttgaat
     5641 ataggcgcaa ggaggcttat gaggttcttt tttgatgctt cttttccttc gctgtggacg
     5701 ttgagagcct tcccaattct ttctaataaa acagtattgc tttcatcgac aagaactgca
     5761 gcgcttctgg tggccccacc ctctactcct agatagaatt tcctcatttt tttcataaaa
     5821 tcactataac acaaggtttg ttaaagagaa tagtgggtct ttgacttttt aatgagcttg
     5881 tctttgagca gggcttgggt aacctcttgt ggggtataag aggaatctgc cagctctttt
     5941 ttgagaaacg tgtgtagctc cttatgcgaa actttgttgc ttggactggc taagataaaa
     6001 cgcacggctt tggtgcggaa agaacgaaaa gagcccgcga atttgctttg cttggcgtaa
     6061 tgg
//
LOCUS       DASRUP010000091         7193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1892788, whole genome shotgun sequence.
ACCESSION   DASRUP010000091 DASRUP010000000
VERSION     DASRUP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892788"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..1451)
                     /locus_tag="VFE94_03635"
     CDS             complement(396..1451)
                     /locus_tag="VFE94_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50206.1"
                     /translation="MTGDYAYKLDQDLTTTKATWTGANSAMIIRFGGDGPESQYEALW
                     QMSGALGSAPPSWRVGATSVIAITTDAPFHDSDSEGAYPGHGGVATTAALVANGRRVI
                     AIKAPGSTGQMDAIATATGGSVVTTGASSAEIADAILQGLGNLPVTVTPSVVGCDPLV
                     VSFAPASKTVTSGDDAVFMETIAVPNDASLEGTSVDCTVVFVDENGNVLGTQRITVKI
                     PDTTPPVAGCEETNNPSGGNVPGAPGKGGQGQNQDGFYVLSAVDRVDPDPALYLVDKG
                     ADNVFGTADDATFGPFSSPTKIKYTEANGATPTQDPGAGDIDWKIKGQGDAAVYAVDA
                     SGNTSAVVECLVPPKPQ"
     gene            complement(2134..3723)
                     /locus_tag="VFE94_03640"
     CDS             complement(2134..3723)
                     /locus_tag="VFE94_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50207.1"
                     /translation="MKNNIIVGVVGFFGLTLTVAIVLAATGDLLATVDLPGNGECNVG
                     IAFDGTHVMTIDAGFGTGCASSTIDIYTPPPASGAAALVSTKTVKDGAGAPVSLSAID
                     WDATRGKLWGAYAGSVYLVDIVGTGIGVTEDVVATLEFNPAVGGIPLIDGLAWDPNDD
                     TLYYSPDVDCNVYQFSLGTGVNPPKGTLMNTVTPKDAAGNADCAVSGVAIGAANSLYI
                     GRDGNAEIRRVNKTTGAFVSTFATTSGRVEDLVCDPVTYAPKEAILAKDAYGSGGIGG
                     GAGDELYEAFEVEVGTCPLPAPEEITLDPPTATNDAGTDHTVTATITQGGQPVVGTLV
                     SFSVTAGPNVGEVSDPGECSVDPNCNTDANGQTSWTYTSNGSTGVDIIEACFSDTTGK
                     EQCARAEKEWIDATPPEAACVETVNPAGKQKPQAPGTGQNEDGFYEVSATDNVGVASF
                     SVVDMGADNILGTADDTVFVVASGDKMKYTEANGATPSIKPGAGVIDWQIKGQGDAAV
                     VAVDAAGNQSVAECLVPNPPK"
     gene            complement(3902..4651)
                     /locus_tag="VFE94_03645"
     CDS             complement(3902..4651)
                     /locus_tag="VFE94_03645"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZX50208.1"
                     /translation="MKVETVKTHKVKPGEDRDLYAVLDRYLPKLKEGSVVAITSKIVA
                     ICQGRYAKIGSVDKGQLIEKESEYFLPPGPNGFYLTVKYGILSASAGIDESNGGGYYI
                     LWPKDPQKTANEIRTYLAKKHGLKNVGVVICDSKITPLRWGVTGVSITHSGFAAMNDY
                     IGTPDIFGRELKFTKTNIMDGLGGVAVMLMGEGKEQTPLAIIEDVPFVNFQQRNPTQK
                     EIKEVCIGMMQDDLYCAILTGVKWRKGGSGI"
     gene            4734..4922
                     /locus_tag="VFE94_03650"
     CDS             4734..4922
                     /locus_tag="VFE94_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50209.1"
                     /translation="MFLIPLLIIGLILGAFYFDDVLGNVNLSIPGQDTGFDIRSILGP
                     EREQGTPIEQLDNPEQPQ"
     gene            complement(4950..6401)
                     /locus_tag="VFE94_03655"
     CDS             complement(4950..6401)
                     /locus_tag="VFE94_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZX50210.1"
                     /translation="MRRNAMYFNKILVADRSDAACRVIRTCMGLRIQTVAIFTEQESN
                     ALHADMANEAYAVTSYSNAQEVMQIARQTGAEAIHPGYGWFSENPLFVRQCIDAGFVF
                     IGPSPRVLLQMGNKIKAKNAAKRAGVPVLPGSESLAYEEEQAILIAAHKIGFPAIVKA
                     AWGGGGRAMEIAETEAELLATVRSIRGMAERFFSEPDLYLEKLVRGGRHIEIQVERDM
                     HGTCFHSRERDCSVQRRRAKIVEEAPAPGLNKRLRRKLCSWAVKLVSKIGYVGVGTVE
                     FLVSPEGKAYFIEMNPRLQVEHGVTEMREGIDLVEVQLAIAAGKKLSTQKKERYKHVM
                     EVRIYTETLRNGIFSPAIGKVIINKFPEPASFLRIDSALRQGQEIECTVKDEDAALIC
                     KLIVGASSREQAIERMIAALRDLRIEGVTGREILLAILHWDSFRKNIHALDTLNNRSV
                     TQEIERAAHRFTLEKGLYPSLGRIAGDVAHEVL"
     gene            6422..6982
                     /locus_tag="VFE94_03660"
     CDS             6422..6982
                     /locus_tag="VFE94_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZX50211.1"
                     /translation="MTREEAKGLLYQYIANEGLRRHHLAAEATMRAFAKRFGQDEELW
                     GLSGLLHDLDWEKIGGDSAQHTKVTEQILREHGVIDEVLINAIKAHHPSASGREPETL
                     QEKVLYSTEEITGLVTAAALVQPDKKLANVTVESLMKKLKNKNFAAGVNRDIVAKSVE
                     YTGMPLEEVVQTVLVAMQEIHEELGL"
BASE COUNT         1670 a         2013 c         1706 g         1804 t
ORIGIN      
        1 ccaaccgagg gcccgtcggg gcacgtggtc caaaccgcgt cgcctccgag gctggaagcc
       61 ttaaagctaa acttttcggc ggccagggcc ttgtctggcg caaacgtcgg cagcagcgac
      121 aacatcgcta ctgcaaaaac caaagtggct agccatttct tattcatttt ctgaatctcc
      181 ctctcgtaat acgttccatt tttttaccca tgttcccgga gaaccacgga atctaaacaa
      241 gcaaggcaac cacctccttt cgcataagct ccctatctct aacgcagcac attttcgttt
      301 gttacaaaaa aaacagaggc agatacatac gtgtatacaa gacacatata atatctgccc
      361 ctttgttact agagttttat gtccttaagg tactattatt gaggcttggg tggaaccaag
      421 cactcgacga cagccgacgt attgccggaa gcatcaacag cgtaaaccgc tgcgtcgccc
      481 tgacccttaa tcttccagtc gatgtcgcca gctccgggat cctgggtggg cgtggcgcca
      541 ttggcctcgg tgtacttgat cttggtcggg cttgagaagg gtccgaatgt cgcgtcgtcg
      601 gcagtgccga aaacgttgtc ggcgccctta tccacaaggt atagagccgg gtcagggtca
      661 acccgatcga cagcgctcaa gacgtagaaa ccgtcctgat tctggccctg accgccctta
      721 ccgggagccc cggggacatt ccctccgctt ggattgttag tctcttcgca accagcaact
      781 ggcggagtgg tatcaggaat cttcacggtg atacgctgtg tacctagaac gtttccattc
      841 tcgtccacaa aaaccacggt acaatccacg gaagttccct ccaaacttgc gtcgttggga
      901 accgcaatgg tctccataaa cacagcgtcg tctccgcttg taacggtctt tgaggcggga
      961 gcaaagctca ctaccagagg atcacagcct accacgcttg gtgtaaccgt aacgggcaga
     1021 ttgcccaacc cttgtaagat cgcatctgcg atttctgcgc tcgaagcacc ggtcgtcacc
     1081 accgaaccgc cggtcgcggt agcaatggca tccatttggc cggtagagcc gggggctttg
     1141 atagcaatga ccctacggcc attggcaacc aacgcggcag tcgttgcaac gccgccgtgg
     1201 cccgggtagg cgccttccga atcactgtca tggaacggag catccgtcgt aatggcaatg
     1261 acactcgtgg cgccaactcg ccaagaaggc ggagcggaac caagcgcgcc agacatctgc
     1321 cacagggctt cgtactggct ttcgggacca tcgccgccaa aacgaatgat catggcgctg
     1381 ttggcgccgg tccacgtcgc tttagtggtc gtcagatcct gatccaactt ataggcatag
     1441 tcgccagtca cggcgcttcc gtacggtgag acagggaagt ccacaaaggt agccgtgcag
     1501 aactgggagt cagccacttg ggcccgaatg tcgtcgaaga ggccgtcgtc ggccgctaag
     1561 atgttgggca ggtcatcgat atagctgccc gagttgtcga cgagcaaaca cagatccaat
     1621 acaggcggga tcgtcggggt tccgacgact ttatcgatat taaccgactc gccgggggca
     1681 agaaccacgt tcacctcggg cggaaatacc tgggcattaa ccacgttggc gctaaatgcc
     1741 gcgagggcaa caagcgccag gaccatcgcc accacggcga atttactaaa tccgggaatt
     1801 gagcttgcgc tcatagtcta acctctcttt atttttttgt ttccccccag gataagacta
     1861 actaggcatc aagaacacac aattcatcca cctcccgtct atttttctct tacgagaagt
     1921 ttgcttttaa agagcattct tctcgactcg agaatttgac ccgcaaaaaa atacaggccc
     1981 aactctcaag tcgagactgt gtttctctcc aaaaacacac tggcacaaag tatatcctat
     2041 acataaaatc ctgtcaagga aagctatcca caacaaaacg ggcccccgac gatactatcg
     2101 agaaagcccg tttgttcatt cagtcaattc agcttatttt ggcggattcg gaactaagca
     2161 ctcggcaacc gactgatttc cagcggcatc aactgcaaca actgcagcat cgccctgccc
     2221 tttaatctgc cagtcgataa cgccggctcc cggcttaatg ctgggtgtag ctccgttcgc
     2281 ttcggtgtac ttcattttgt cgccacttgc taccacaaat accgtgtcgt cagctgtacc
     2341 gagaatattg tccgcaccca tatctaccac agaaaagctt gctacgccaa cattatctgt
     2401 cgcagacact tcatagaatc cgtcctcgtt ctgcccggtt cccggcgctt gcggcttctg
     2461 cttgccagct gggtttaccg tttctacaca tgcagcttcc ggcggtgttg cgtctatcca
     2521 ttctttttca gctctcgcac actgctcttt gccagttgtg tcactaaagc aagcctcaat
     2581 gatatcgact cctgtgcttc cgttgctcgt gtacgtccac gaggtttgcc cattggcatc
     2641 ggtattgcag ttgggatcga ccgaacattc gccaggatcg ctcacctcac ccacatttgg
     2701 gccggcagta accgagaaac tcaccaaagt cccaaccaca ggctgtccgc cctgggtaat
     2761 agtggcggtc acggtgtgat cggtacccgc atcattcgtt gcagtcggcg ggtccagcgt
     2821 aatctcttct ggcgcaggca gcgggcaggt gccgacctcc acctcgaagg cctcatagag
     2881 ctcatcgcca gccccgccac caatgccgcc cgaaccatag gcgtcctttg ccagaatggc
     2941 ctccttgggg gcgtaagtga ctgggtcaca gaccaggtcc tctacccgac cggaggtggt
     3001 agcgaaggtg gatacaaagg cgccagtggt tttgttcact cggcggattt cggcgttccc
     3061 gtcccggccg atgtacagac tgttcgcggc accgatggcc acaccgctga ccgcgcagtc
     3121 tgcgtttccg gccgcgtcct tcggcgtcac ggtattcatt agtgtgccct taggtgggtt
     3181 tacaccggtg cccagcgaga actggtaaac gttacagtct acatctggac tatagtagag
     3241 cgtatcatcg ttggggtccc aggccagtcc gtcaatcaga ggaatcccgc ccactgcagg
     3301 gttgaactcc agcgttgcca cgacgtcttc ggtgacgccg atgccggtcc cgacgatatc
     3361 taccaggtag acgctaccgg cgtacgcgcc ccatagcttg ccgcgagtgg catcccagtc
     3421 tatagcgctg agggacaccg gggcgcccgc gccatccttg acagtcttgg tggataccag
     3481 ggccgcggca ccgcttgccg gaggcggggt atagatatca atagttgaac tggcgcaacc
     3541 agtgccgaag ccagcgtcga tcgtcatcac atgggtgcca tcgaaggcga tgccaacgtt
     3601 gcactccccg ttgccgggca agtcgacagt ggccagcagg tcaccggtcg ccgcaagaac
     3661 tattgctact gtcaacgtca acccgaaaaa tccgaccaca cctacgatta tattgttttt
     3721 cattgttgtt tgattttact gttgtaatgt tgaacttttg ggttcgacct ttgcgctaac
     3781 aaaaaacccc acacagaaat ttcctctcac gaatttctaa aaggggtcct ctagctccaa
     3841 ttctatcaac tctattttcg cttatcgcag ctaagtgtca agcatatctt ttccacagac
     3901 tttaaatacc gcttccgcct ttgcgccatt tcacgccagt gagaattgca caatagagat
     3961 cgtcttgcat cataccgatg caaacttctt ttatttcttt ttgtgtggga tttcgctgct
     4021 gaaagtttac aaacggcaca tcttcaatta tcgccaatgg ggtttgttct ttgccttcac
     4081 ccataagcat aacggcgact ccgcccagac catccataat atttgttttg gtaaacttta
     4141 gttcgcgccc aaaaatgtcg ggcgtgccaa tataatcgtt catagcggca aaaccgctgt
     4201 gggtaatgga aactccggtt actccccagc gcaaaggcgt aattttgctg tcgcaaataa
     4261 ccacgccgac attttttaaa ccgtgttttt tggcaagata tgtacgaatt tcatttgcgg
     4321 ttttttgcgg gtctttaggc cacaaaatat agtatccccc gccgtttgac tcgtctattc
     4381 ccgcggacgc cgacaaaata ccgtatttca cggtaaggta aaacccgttt ggcccgggcg
     4441 gcaaaaagta ttctgattct ttttcgataa gctgcccctt gtctacgctg ccaattttcg
     4501 cgtaccttcc ctggcaaata gccacaattt tcgaggtaat ggccaccacc gagccttctt
     4561 tgagctttgg aagatagcga tccaagactg catacaagtc tcggtcttcg cccggcttta
     4621 ctttatgggt ttttactgtt tctactttca tggaaatacg cttttatagt tgcataaacg
     4681 gcggtttttg acaaacacat gtatttcccg tatcgtaaaa acaatactcc cttatgtttc
     4741 ttattcccct tcttatcatt ggtcttattc ttggcgcgtt ttacttcgac gatgtgttag
     4801 gcaacgttaa cctttctata cccggccaag acaccgggtt tgacattcgc agcattctcg
     4861 gccccgaacg cgagcaaggc actcccatcg aacaactcga caatccagaa caaccacaat
     4921 aaaaaagctc tgccgttcca atgacagagt taaagaactt cgtgtgctac gtcgccagca
     4981 atccggccaa ggcttggata caggcctttc tcgagagtga acctgtgtgc ggctcgttct
     5041 atctcctgtg ttacactgcg gttgttcaga gtgtctagcg catgtatgtt tttacggaac
     5101 gaatcccagt gcaaaatagc caatagtatt tcccggcccg ttaccccttc gatccgcaag
     5161 tcgcgaagcg ccgctatcat tcgctctatt gcctgctcgc gagaggaagc acccactatg
     5221 agcttgcaga taagcgcggc atcttcatct ttcaccgtgc attcaatttc ctgtccttga
     5281 cgcaaagcgc tgtcgattcg cagaaaactg gctggctcgg gaaatttgtt gattatcacc
     5341 ttgcctatgg cggggctaaa aatcccgttc cgcaaagttt ccgtgtagat tcgtacctcc
     5401 ataacgtgct tgtatctttc tttcttctgt gttgacagct tttttccggc cgcaatagcc
     5461 agctgaactt ccacaaggtc gataccttct cgcatctcgg taacgccgtg ctcaacttgt
     5521 aagcgcgggt tcatctctat gaaataggct tttccctccg gagagacaag aaattccacc
     5581 gtgccaacgc ctacgtaacc tattttgctc accagcttta cagcccagct acacagtttc
     5641 ctgcgtaatc ttttgtttaa ccctggcgcg ggggcttctt ctacgatttt tgctctgcgg
     5701 cgctgcaccg aacaatcccg ttctcgcgaa tggaagcagg taccgtgcat gtcgcgctcc
     5761 acctgtattt caatgtggcg gccgcctctt acgagcttct ctaagtaaag gtctggctcg
     5821 gagaaaaatc tttctgccat accgcgtatg cttcgcaccg tagccagaag ttctgcctcg
     5881 gtttcagcaa tttccatggc tctgccgcca ccgccccacg ctgcttttac tatagccgga
     5941 aatcctattt tatgggcagc aataagaatg gcctgctctt cttcataagc aagactttct
     6001 gaaccgggga gtaccggaac tcccgctctt ttcgcagcgt tttttgcttt gattttattg
     6061 cccatttgca aaagtacgcg cggactcgga ccaatgaata caaacccagc gtcgatgcac
     6121 tggcgtacga acaaggggtt ttcggaaaac caaccgtagc cagggtgaat ggcctctgct
     6181 ccggtttgtc gggcaatttg cattacctct tgggcgttac tatagctggt taccgcatag
     6241 gcttcgtttg ccatgtcggc atgcaaagca ttgctctctt gctctgtaaa aatggcaacc
     6301 gtttgaatac gcagccccat acaggttctt atcaccctgc aggcggcatc gctccggtct
     6361 gcaaccagaa ttttgttaaa gtacattgca ttcctcctca tttcctttta tcattatttc
     6421 aatgactcgg gaagaagcaa aaggactctt ataccaatac attgcgaacg aaggcttgcg
     6481 gcggcatcac ttggctgcag aagccaccat gcgcgctttt gccaaacgct ttggccaaga
     6541 cgaggagctt tgggggcttt ctggcctgct gcacgattta gactgggaaa aaattggggg
     6601 cgattctgcg cagcacacca aagttaccga gcaaatattg cgcgagcacg gcgtgataga
     6661 cgaggtttta ataaacgcca taaaagccca ccatccgtcg gcttctggcc gggaaccgga
     6721 aaccctgcaa gaaaaagtac tctatagcac cgaagaaatt accggcttgg ttactgctgc
     6781 ggctttagtg cagccggata agaagctggc caacgtaacg gtggagtctt taatgaagaa
     6841 gctcaaaaac aagaactttg cggcaggagt aaatcgcgat attgtcgcca agagcgtgga
     6901 atacaccggt atgcctcttg aagaagtggt gcaaaccgtg cttgttgcta tgcaggaaat
     6961 ccacgaagaa ctaggccttt aactaaattt ttccggcttc aatttgcttt cgcaagtcgg
     7021 caaagaattc agcgaagacc ctggactttt tactttgttt agtaacctta aaaaacgcca
     7081 ttgaatacta cagtgtcgcc gacggttacg ccgtgagcct gcgcccagcc ggcgtttacc
     7141 tcgagcacat actttacaag agaaggcgga aagaaaattt caggataact ttc
//
LOCUS       DASRUP010000092         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1364522, whole genome shotgun sequence.
ACCESSION   DASRUP010000092 DASRUP010000000
VERSION     DASRUP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364522"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFE94_03665"
     CDS             complement(<1..211)
                     /locus_tag="VFE94_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZX50212.1"
                     /translation="MATDTIVKENIKQQLPDIQPGDTIRVHQKVKEGPSTGSGQAKER
                     IQVFEGLVLARKHGKGINATITVRKI"
     gene            complement(201..830)
                     /locus_tag="VFE94_03670"
     CDS             complement(201..830)
                     /locus_tag="VFE94_03670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZX50213.1"
                     /translation="MPLKILHEDATLLVVDKPAGLTVEEIARQLGYTPAHRLDKDTSG
                     VLLVAKNEESLESLRRRFKEREVEKHYICLVEGDLKEDQGIIHTLLTRSPADRRKQRT
                     YPLNEPKEGRREAITEWKVLQRLQDYTLLQARPKTGRKHQIRAHMVQLGHPIAGDKLY
                     GFKNQHIPRGLTRQFLHAGYLKIASQEFVSELPEDLQNVLELLPSHYGN"
     gene            complement(832..1527)
                     /locus_tag="VFE94_03675"
     CDS             complement(832..1527)
                     /locus_tag="VFE94_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50214.1"
                     /translation="MKNIQQYLTTRVLLFIAIFAVAGFAALQIPVTQLAGSGAKFTAF
                     DAFAPLSGAFIGSIPGVAAVLFMQAANFAVHGAQVQDVGTIIRFFPMLFAVLYFARKG
                     KLNLLVPLLAMAAFIAHPIGREVWYFSLFWTIPVICYFLRDRFLLARALGATFTAHAV
                     GGAAWIWAFSLPAPVWNSLIPIVIVERLLFALGIAASYVVITNALYALERKKILKLGF
                     SLDHKYLLPWKLA"
     gene            complement(1530..1603)
                     /locus_tag="VFE94_03680"
     tRNA            complement(1530..1603)
                     /locus_tag="VFE94_03680"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1567..1569),aa:Val,seq:gac)
     gene            complement(1607..1682)
                     /locus_tag="VFE94_03685"
     tRNA            complement(1607..1682)
                     /locus_tag="VFE94_03685"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1647..1649),aa:Ile,seq:tat)
     gene            complement(1673..>2290)
                     /locus_tag="VFE94_03690"
     CDS             complement(1673..>2290)
                     /locus_tag="VFE94_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HZX50215.1"
                     /translation="SHVLSKKEKEVTAYHEAGHALVAASLRHVDPVRKISIISRGRAG
                     GYTLKLPTEDRHMKSRKEFLAELAVLLGGYVAEKMVFGDTTTGPSNDLEKASEIARSI
                     VKDFGMSAKLGPVTFGEKEHLPFLGRDMESAKNYSETTALEIDKEVSILIEDARKTAE
                     QVLRQKRKTLDKIAKVLVEKETIEREEFEELVGKPTKSSSRKKGM"
BASE COUNT          519 a          580 c          573 g          618 t
ORIGIN      
        1 ttatttttcg cacggtaatg gtggcattga ttcctttgcc gtgctttctg gccagcacca
       61 aaccttcgaa cacctggatg cgttcttttg cttgccctga gcctgtcgaa gggccttctt
      121 ttactttttg gtgtacgcga atcgtatcgc caggctggat gtctggaagt tgttgtttga
      181 tattttcttt tacgattgtg tcagttgcca taatgcgatg gtaatagttc taatacgttt
      241 tgcaaatctt ccggtagctc cgaaacgaat tcctggctcg ctattttcag atagcccgcg
      301 tgcagaaatt gtctggttaa accccgggga atatgctgat ttttaaaccc gtagagtttg
      361 tcccctgcaa tgggatggcc aagctgaacc atgtgcgctc gaatctggtg ttttctgcct
      421 gttttcggcc ttgcctggag taatgtatag tcttggaggc gttgcagaac tttccattcg
      481 gtaattgcct cccggcggcc ctcttttggc tcgtttaagg gatacgttcg ctgctttctg
      541 cggtcggccg gcgatcgggt aagaagagta tgaataatcc cttggtcttc tttaagatcc
      601 ccttccacca ggcaaatgta atgcttttcg acttcgcgct ctttaaaccg tctccggagc
      661 gattctagag actcctcgtt tttggccacc agcaggacgc ccgaggtatc tttgtctaac
      721 ctgtgcgccg gggtgtaccc cagctgccgg gcaatctctt ctacagttaa gcccgctggc
      781 ttatcaacaa ccaatagtgt agcatcttcg tgaagaattt tcaagggcat ttcaggctaa
      841 cttccaggga aggaggtatt tatggtctaa agagaaccca agcttaagaa ttttctttcg
      901 ttccaaggcg tagagcgcat tcgttattac aacgtagctt gctgcaatgc ccagggcaaa
      961 caaaagtcgt tctacaataa ctatggggat taacgagttc cacacaggag ccggcagcga
     1021 gaaagcccaa atccaggcgg cccctcccac cgcgtgagcc gtaaacgtgg ctccaagcgc
     1081 ccgcgccagc aaaaagcgat cgcgcaggaa gtagcagatt accggaatgg tccagaacaa
     1141 agaaaaatac catacttccc gtccaatagg atgagctata aatgctgcca tcgcaagaag
     1201 gggaaccaaa aggttaagct ttcccttcct ggcaaagtaa agcaccgcaa acagcatagg
     1261 gaagaatcga ataatcgtgc ctacgtcttg cacttgggcg ccgtgcaccg caaaattcgc
     1321 cgcctgcatg aaaagcaccg cagctacgcc cggaatgctg ccgatgaatg ctccgctcaa
     1381 gggggcaaaa gcgtcgaacg cggtaaattt agccccagac cctgcaagct gcgtgaccgg
     1441 gatttgcagc gccgcaaacc ctgccaccgc aaagattgca ataaagagca gtacgcgtgt
     1501 tgtgagatat tgttgtatat ttttcatagt gggcggtaga gggatcgaac ctcttacctt
     1561 cacaatgtca atgtgacgct ctgaccaagt gagctaaccg cccaaatggt gggcatgcct
     1621 ggactcgaac caggaaccac gcgtgtataa gacgcgagct ctaaccattg agctacatgc
     1681 cctttttccg agaggaggat ttggttggtt tgcctacaag ctcttcgaat tcctcgcgct
     1741 cgattgtctc cttttctacc agaactttgg ctattttgtc cagagtcttg cgtttttggc
     1801 ggagcacttg ttccgccgtt tttcttgcgt cttcgataag gattgaaacc tctttgtcta
     1861 tctccagcgc cgtggtttca gagtagtttt ttgcagattc catgtcgcgc cccaaaaagg
     1921 gcaggtgttc tttttcgcca aaggttacgg ggcctagttt cgcggacatg ccaaagtctt
     1981 tcacaatact tcttgcgatt tcagaagcct tttccaagtc attgctgggg ccggtggtgg
     2041 tgtcgccaaa caccattttc tctgccacat acccaccgag gagcacggcg agttcggcaa
     2101 gaaattcttt gcgcgatttc atgtgtctgt cttcggtggg aagttttagg gtatacccgc
     2161 cggctctgcc gcgcgagata atagaaattt tgcgcaccgg atctacatgc cgaagggagg
     2221 ctgcaaccaa agcgtgcccc gcttcgtggt aggcggtaac ttccttttct tttttggaaa
     2281 gtacgtggct
//
LOCUS       DASRUP010000093         5345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_578579, whole genome shotgun sequence.
ACCESSION   DASRUP010000093 DASRUP010000000
VERSION     DASRUP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578579"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VFE94_03695"
     CDS             <1..1341
                     /locus_tag="VFE94_03695"
                     /inference="COORDINATES: protein motif:HMM:NF028498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HZX50216.1"
                     /translation="PPPTSTGTVTVNANTKFQTISGWEATSQSGQDSPEFSQYANTLF
                     DRAANDLGINRIRLEVRSGMENDTDYWSLFDNGNGSLSSAEWRCVRYSTKNDNSSHTS
                     INPSGFKFSELDDKVNKIVKPLQQKLQARGERLHINLNYVAFTKQITGSGCSSSLQYH
                     HDDPDEYAEFILAASLHLRGMGITPDTWEIILEPDNVPEWDGRLIGQAILATAQKLEA
                     NGFSNPKFIAPSNTNMGSAISYFDAMANVLGSSNVTKYVKEFSYHRYGGVSDTNLQTI
                     ASRASQYGIGTSMLEHIGSGYEDLHKDLKLANNSAWQQFTLAFPASDNGGHYYTIGGS
                     PGNPTVNIGSRTKFLRQYFKFIREGAVRIKADSTNNNFDPVAFINKNGKYVVVVKANS
                     GGAFTVGGLPAGPYGITYTTGSQYNQSMPDVTISNSQAVNANIPSGGVITIYRR"
     gene            1344..1967
                     /locus_tag="VFE94_03700"
     CDS             1344..1967
                     /locus_tag="VFE94_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50217.1"
                     /translation="MPTQKVLLIILAILVFVSAFFWLVVLRSDAPAGWERYSNNQLGF
                     QINHPAEMEVTSLPESEGATFSLSGNEEQSIAGFARFSDGIQLTVTRQLLSGEPFLDV
                     VNAKNSAAELIQLGNAQGYTYESTNIVGDGIVLILVEAPQDQFFEISYNVQDPNGRGY
                     QQIVDQAAASFEILESGVPFALTIVFGIIILAGIALGAGLLFISRPT"
     gene            complement(1973..2956)
                     /gene="trpS"
                     /locus_tag="VFE94_03705"
     CDS             complement(1973..2956)
                     /gene="trpS"
                     /locus_tag="VFE94_03705"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZX50218.1"
                     /translation="MRVLSGIQPTGQLHIGNYLGAVKYWAQLQEKHNCYFAVVDLHAL
                     TVQQDPKAFAKTTMDKVIELLSVGLDPERCTLFLQSSIKEHTELAWIFNTLIPMAELE
                     RMTQYKDKAEKNQKNVNAGLFTYPALMAADILLYKTDGVPVGKDQEQHLELTRLVARK
                     FNNLYGKTFEEPDSLIPNEGAKVMSLQDPTKKMSKSDAPASYISLFDSPEDIEKKIMR
                     ATTDEEDAVTYNPAKKPGVSNLLQIYSLFAQEPVKDLEKKFQGQGYASLKKETARLLA
                     DTLEPFRRKKKELESRDLYVREILKRGAKKASLQAASTMEEVRNKIGLVSL"
     gene            complement(2964..4694)
                     /locus_tag="VFE94_03710"
     CDS             complement(2964..4694)
                     /locus_tag="VFE94_03710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZX50219.1"
                     /translation="MEKEASRPPTTFATRKSAGQPKDMNLRITPLGGLGEVGRNLMVV
                     EYQNSILLIDLGFRMPEEDMPGVDYIIPNISSLKGKEKQILALLITHGHYDHIGAIPY
                     LLRQLGNPPIFAGNLGKAIILKRQEDFATQAKLRITALEDGKKFKLGPFTVEPFRQNH
                     NITDNFGFFINTPAGNIVHTSDFKFDDDPVNEPGTDYKRLQKIGDQGIHLLMSDSTNA
                     EEPNHSLSEKVIFANLDEIFKQAKGRIITATFASLINRVQQIITLSEKYGRHVGLEGY
                     SMKTNVEISKQFGYMKSKAGTILKTKDVLSYPDSKVTICGTGAQGEERAVLMRIVNGE
                     HPHVKIQRGDTVIFSSSVIPGNERSVQFVKDQLYRQGANVFHYKMMDIHASGHANQEE
                     LKQMLKLMRPKFLMPVHGQFSMLVNHAKLAQGEGMPEQNIFVMENGQTLSLNKAEAAI
                     QKTKAPANYIMVDGLGVGDVGEVVLRDRQNLAKDGIFVIICVVDRQTGKVKGSPDIIS
                     RGFIYLRESKELLREVRKKTIATVDQSSGSGGPINWIYVRDLVRNRISEYLFTKTERR
                     PMVLPVIIEV"
     gene            complement(4728..4811)
                     /locus_tag="VFE94_03715"
     tRNA            complement(4728..4811)
                     /locus_tag="VFE94_03715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4775..4777),aa:Leu,seq:cag)
     gene            complement(4863..>5345)
                     /locus_tag="VFE94_03720"
     CDS             complement(4863..>5345)
                     /locus_tag="VFE94_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50220.1"
                     /translation="QPPLRQVAQELERQWELFGIQTEIQLSPAASLERDVIKPRAYQT
                     LLFGEVLGKIPDPFPFWHSSQVTDPGLNLSSYQNQELDKLLEAARREPEEEKRKAMFE
                     QAQSILLADVPALFLYDLPYTYFVSKKIQGVQPSVIANPSWRFAGVGEWYIETRRAGK
                     "
BASE COUNT         1280 a         1287 c         1339 g         1439 t
ORIGIN      
        1 cctcctccga cttctaccgg cactgttacc gtaaacgcaa acacgaagtt ccaaaccatc
       61 tcgggctggg aagccacgtc ccagtcgggc caggatagtc ccgagttttc gcagtatgcc
      121 aatacgctgt ttgacagggc ggcaaacgac ctgggcatta acagaatacg cctggaagtg
      181 cgctctggaa tggaaaacga cacagactac tggagtttat tcgacaacgg caacggatct
      241 ctttctagcg ccgagtggag atgcgtgcgc tactccacga aaaacgataa cagttctcat
      301 acgtccataa acccaagcgg tttcaagttt tccgaactgg acgacaaagt aaacaaaata
      361 gtcaaacctt tgcagcaaaa actccaggca agaggagaga gactgcatat aaacctaaac
      421 tatgttgcat ttaccaaaca gattaccggt tctggatgca gttcttcttt gcaataccat
      481 catgacgatc ctgacgaata cgcggaattc atactggccg ccagtttgca cttaaggggt
      541 atgggcatta ctcccgatac ctgggaaatt atcctggaac ccgacaacgt ccccgaatgg
      601 gacggcaggc tcataggaca ggccatattg gccaccgccc aaaagctgga agcaaacggt
      661 ttttccaatc ccaagtttat cgccccctcc aacacaaaca tgggaagcgc catttcgtat
      721 tttgatgcta tggcaaatgt tctgggttct tcaaacgtta ccaaatacgt taaggagttt
      781 tcttaccacc gctacggagg cgtttctgat acgaaccttc agactatagc cagcagggcg
      841 agccagtatg gcattggcac gtccatgtta gagcacattg gcagcgggta tgaagacctg
      901 cataaagact taaaactggc aaacaactcg gcctggcagc agtttaccct ggccttcccc
      961 gccagcgaca acggtggcca ctactacacc atcggaggca gtccgggaaa cccaaccgta
     1021 aacattggtt cccgcaccaa attcctgcgc cagtacttta agtttatccg cgaaggcgcg
     1081 gtgcgcatta aagcagactc aaccaacaac aactttgatc cggttgcgtt tattaacaaa
     1141 aacggcaaat acgtggtggt ggtaaaagca aattcgggcg gagcctttac agtgggaggc
     1201 cttcccgcgg gcccgtacgg cattacctac accaccggca gccagtacaa ccaaagcatg
     1261 ccggacgtta ccataagcaa cagccaggcc gtaaacgcca acatcccttc gggcggcgtt
     1321 attaccatat atcgaaggta gctatgccta cgcaaaaggt tttacttata atattagcca
     1381 ttcttgtctt tgtatctgcg ttcttttggc tggtggtttt gcggagcgac gcgcctgcgg
     1441 gttgggagcg gtactccaat aaccagctgg gctttcaaat aaaccacccg gcggaaatgg
     1501 aggttacgtc tcttcccgaa agcgaaggcg cgactttttc tttatctggg aacgaagagc
     1561 aaagtatagc gggattcgcg cgtttttcag acggcataca gcttacggta acaaggcaat
     1621 tactttccgg cgaacctttc ttagatgtgg taaacgcaaa aaacagcgcc gcggaactta
     1681 tacaattagg gaacgcgcaa gggtatacgt atgaaagtac aaatatagta ggtgacggta
     1741 ttgtcctcat tcttgttgag gccccgcaag atcagttttt tgagatatcg tataatgtgc
     1801 aggatcccaa tggacgggga taccagcaaa ttgttgacca agcggcggct tcttttgaga
     1861 ttttggaaag cggggttcct ttcgcgctga ctatagtttt tggaattatt atacttgcgg
     1921 ggattgcgct cggcgccggt ctgttgttta taagcaggcc gacttagcgg tttcagagag
     1981 aaacgaggcc gattttgttt cttacctctt ccatggtgct ggcagcttgt aaagaggctt
     2041 tttttgcgcc gcgctttaag atttctcgca cgtagaggtc tcgggactcc agctcttttt
     2101 tcttgcgtcg aaaaggttcg agcgtatctg ccaagagccg ggctgtttcc tttttaagag
     2161 aggcatagcc ctgcccttgg aatttttttt cgaggtcttt tactggttct tgcgcaaaca
     2221 aagagtatat ctgcagcagg ttcgatacgc ccggcttttt ggcgggatta tacgtaacgg
     2281 catcttcctc gtcggtggta gcccgcataa ttttcttttc gatatcttcg ggcgagtcga
     2341 acagcgaaat ataagaggcc ggggcgtctg acttcgacat tttcttggtt ggatcttgca
     2401 aagacatgac tttcgctccc tcattgggaa ttagagaatc aggctcttca aacgtttttc
     2461 catagaggtt gttgaatttt cttgcgacaa ggcgcgtaag ttcgaggtgc tgctcctggt
     2521 ctttccctac gggtacgcca tcggtcttgt acaataaaat gtcggccgcc attaaggcag
     2581 gataggtaaa gagcccggcg tttacgtttt tctggttttt ttcagctttg tctttgtact
     2641 gcgtcatgcg ttccaactct gccataggga taagggtgtt gaaaatccag gccagttcgg
     2701 tgtgctcttt tatggaagac tgcaaaaaga gagtacagcg ttcaggatcc agtcctaccg
     2761 aaagaagttc tatcacctta tccattgtag ttttggcaaa ggctttcggg tcttgctgca
     2821 ccgtgagcgc atgcaaatct accactgcaa aatagcagtt atgtttttcc tgcagctgcg
     2881 cccagtattt taccgctccc agatagttcc caatatgcag ttgtccggta ggctgtatgc
     2941 cgcttaagac tctcatgcct caattatacc tcaatgataa cggggagaac cataggccgt
     3001 cgttcggtct tggtgaacaa gtattcagaa atccggttgc gcacgaggtc tcttacataa
     3061 atccagttaa taggccctcc agaccccgaa gactggtcta cagtggctat ggttttcttt
     3121 cttacctcgc ggagcagctc tttggactcc cgcaggtaaa taaagccgcg cgaaataatg
     3181 tcgggagagc cttttacctt gccggtttgc cggtctacca cgcaaataat cacaaagatc
     3241 ccgtctttgg ccaggttttg gcgatcgcgc agcaccactt cgcccacgtc gccaactccc
     3301 agaccgtcta ccataatata gttggctggc gccttggttt tttgtatggc agcttccgct
     3361 ttgttaagcg aaagagtctg gccattttcc ataacaaaga tgttttgttc tggcatgcct
     3421 tctccctggg cgagttttgc atggtttact aacatcgaaa actgtccgtg cacgggcatt
     3481 aaaaacttgg ggcgcataag cttgagcatt tgctttaact cttcctgatt ggcgtgtccg
     3541 gaagcgtgga tatccatcat tttgtagtgg aatacattgg cgccctgcct gtagagctgg
     3601 tcttttacga attgcacgct gcgttcgttg cccggaatta ctgaagaaga aaagattacc
     3661 gtgtcgcccc gctgaatttt cacatgagga tgttcgccgt ttacaatgcg cattaacacg
     3721 gcgcgttcct ctccctgggc cccggttccg caaatggtaa ctttggagtc gggatacgag
     3781 agcacatctt tggttttcag aatagtgccg gctttggatt tcatgtatcc aaactgcttg
     3841 gaaatctcca cgttggtttt catggagtat ccttcgaggc ccacgtgcct gccgtatttt
     3901 tccgaaagcg taataatttg ctgcaccctg ttaatcagcg aagcaaaggt ggcggtaata
     3961 atccttccct ttgcctgctt gaagatttcg tctaagttcg caaaaatgac tttttcagaa
     4021 agcgagtggt tcggttcttc ggcgtttgtg gagtcggaca ttaacaaatg aatcccctgg
     4081 tcgccgatct tttggaggcg cttgtagtcg gtgccgggct catttaccgg gtcgtcgtcg
     4141 aacttaaagt cggacgtatg cacgatattc cctgcagggg tattgataaa aaatccgaag
     4201 ttgtcggtaa tattgtggtt ttgccggaag ggttccacgg tgaaagggcc gagcttaaac
     4261 tttttgccgt cttccagggc ggtaatgcgg agtttggcct gggtggcaaa atcttcctgg
     4321 cgtttgagaa taatggcttt tcccaggttt cctgcaaaga tgggtggatt gccgagctgg
     4381 cgcagcaaat agggtatagc tccgatgtgg tcgtagtggc catgcgtaat taaaagagcc
     4441 aggatttgtt tttctttgcc tttgagcgaa gaaatattgg ggataatata gtctacgccc
     4501 ggcatatctt cttctggcat gcgaaagccc aaatcaatga gcaatatgct gttctggtat
     4561 tctactacca tcagattgcg cccaacctca cccaaccctc caaggggggt gatgcgcaag
     4621 ttcatgtctt ttggctgccc tgcgcttttg cgcgttgcaa aagtggtagg cggtctcgat
     4681 gcttctttct ccataaggaa ttagtaatta gagtttatta ttcgaggtgc ccagagcgag
     4741 actcgaactc gcaagccggt gaaggcacta gctcctgaag ctagcgtgtt taccaatttc
     4801 accacctggg cttaaagaaa actgctttgc tttaaggggc ccgccaaagg cgggaacatt
     4861 atttattttc cggctcttct ggtctcgata taccattcgc ctacgccggc aaagcgccag
     4921 gaagggttgg cgattacgga aggctggact ccctgaattt tcttggaaac aaagtaggta
     4981 tagggaaggt cgtacagaaa aagtgcgggc acgtctgcca ataatatgct ttgcgcttgt
     5041 tcaaacatgg ctttgcgttt ctcttcttct ggctctctcc tcgcagcttc cagcagcttg
     5101 tctaattcct ggttctggta ggaagaaagg tttaatcccg gatctgttac ctgcgaagaa
     5161 tgccaaaagg gaaaaggatc tggtattttg cccagcactt cgccaaacag aagagtttgg
     5221 taggcacgcg gctttataac atcccgttca aggctagccg caggggaaag ctggatttcc
     5281 gtttggatgc caaagagttc ccactgccgt tccaattcct gggctacctg gcgtaaaggc
     5341 ggttg
//
LOCUS       DASRUP010000094         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_423981, whole genome shotgun sequence.
ACCESSION   DASRUP010000094 DASRUP010000000
VERSION     DASRUP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423981"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1661
                     /gene="metG"
                     /locus_tag="VFE94_03725"
     CDS             165..1661
                     /gene="metG"
                     /locus_tag="VFE94_03725"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZX50221.1"
                     /translation="MAKFYITTAIPYVNAKPHIGHALEFVQADVIARYKKQQGNDVCL
                     LSGADENALKNVQAAEKAGVPVQQFIDENAVLFGELATKLNVEFHVFQKGSSKEHHAS
                     SQKLWELCNKAGDIYKKTYEGLYCVGCETFYTPDELDKNGECFEHPGRKLEKVQEENY
                     FFKLSTYQEKLVGLIEKDQLRIVPATRKNEVLSFLKQPLQDISISRSNERAKNWGVPV
                     PGDDSQRMYVWFDALNTYQSGIGFGLNEEQYKKWWPADVHVIGKGITRFHAVYWPAFL
                     MSAGLSLPKTIFVHGYITVDGQKMSKTLGNVVDPFVLLEKYGTDAVRYFFLREIPPTE
                     DGDFSFEKFETRYNADLANGLGNLLARLTTMAKTHGGNIESSSISIGNQKLKEGVEEM
                     IDHCQKALDNFQFHEALEAIWKVISQCDQYIEEKKPWEKDREDAGEVWKDLIATLAEV
                     GWLLKPFLPETADRVLEQLGSAQKSREGGTWTIQTKIEKGESLFPRIQ"
     gene            1661..>2736
                     /locus_tag="VFE94_03730"
     CDS             1661..>2736
                     /locus_tag="VFE94_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZX50222.1"
                     /translation="MAKFAYDSTKNADLFYAIKVPVLDPFFFIDLGQAKYVFLDHREL
                     GFFQEKNKNPNLQAVLLKPSGSFEDFAWQLLEQYGLGQGELEVPFNFPLQLADFLRSK
                     GIELSVQESLYPERAIKTTEEVQAIRQSVQKTEKAFALIERILRESTIQENSILYNEE
                     VLTSEFLKSEAEKLLLKEDMISEEGIIISCGQHAAIPHHPGEGGLKPHQTIVCDLFPR
                     SRTTGYFADITRTFVKGAPPEEIQKMYEAVLLAQQEGIKAVAPGKKASDIHKVCIDVL
                     LQAGYHVGDKGFLHNTGHGLGLEVHENPRLGEKSEETLEPGNVETIEPGLYYPERGIG
                     IRIEDDILVTAQGAENLVSYPKGWV"
BASE COUNT          821 a          565 c          701 g          649 t
ORIGIN      
        1 tttccgtttt caaataacca atggacccac cattaaggtg gagcctggag acctcattgt
       61 tcaagagagc caacctttgg aggtggttct ctaagactcg ggaagcacat ttcccgaggt
      121 ttatttttct ggaaaataaa gggaaattgt gctaagagaa aggaatggcc aagttttata
      181 tcaccacagc aattccatac gtaaacgcaa agccccacat tgggcatgct ttggagtttg
      241 tgcaggcaga tgttattgcc cgatataaaa agcagcaggg gaacgacgta tgcctccttt
      301 ccggagcaga tgaaaacgcg ttaaaaaacg tacaagcagc agaaaaagca ggagttccgg
      361 tgcagcagtt tatcgacgaa aacgccgtgc tttttggcga gcttgcaaca aagctcaatg
      421 tagagtttca cgtatttcag aaaggtagca gcaaagagca tcatgcctcg agccaaaaac
      481 tgtgggaatt gtgcaacaag gcaggggata tttacaaaaa aacctacgaa ggtctgtatt
      541 gcgtggggtg cgaaactttt tatactccag acgagctcga caaaaacggc gagtgttttg
      601 agcacccagg ccgcaagtta gaaaaagtac aggaagaaaa ctatttcttt aagctttcaa
      661 cataccaaga aaagttggtt gggttaatcg agaaagacca attgcgcatt gtgcctgcca
      721 cgcgcaaaaa cgaagtgttg tcgtttttaa aacagccctt gcaagacatt agcatttctc
      781 gctcaaacga acgcgccaaa aattggggag ttccggtgcc tggcgacgac agccagcgaa
      841 tgtatgtttg gtttgacgct ttaaacacct atcaatctgg catcggtttt ggattaaacg
      901 aggagcaata caaaaaatgg tggcctgcag acgtgcatgt aataggcaaa ggcattacgc
      961 gttttcatgc cgtgtattgg ccagcgtttt taatgtctgc cggtttgtct ttacccaaaa
     1021 ccatttttgt gcacgggtat attaccgtag acgggcaaaa aatgtctaaa actctgggaa
     1081 atgtggttga ccctttcgtt ctgctcgaga aatacgggac agacgccgtg cgatactttt
     1141 tcctgcgcga gattccgcct accgaagacg gcgactttag tttcgaaaaa ttcgaaaccc
     1201 gctataatgc cgatttggcg aatggtttgg ggaatcttct ggcgcgatta acaacgatgg
     1261 caaaaactca tggcgggaac atcgaaagtt ctagtatttc tattggaaat caaaaactta
     1321 aggagggtgt tgaggagatg atagatcact gtcagaaagc tctcgataac tttcagtttc
     1381 acgaagcgct tgaagcgatt tggaaagtca tttctcaatg tgaccagtat atcgaggaga
     1441 agaagccttg ggaaaaggac agagaagatg cgggagaggt ctggaaagat ctaatcgcga
     1501 cgttagctga ggttggatgg cttctcaaac catttcttcc ggaaaccgct gatagagttc
     1561 tggaacagct aggaagcgcg cagaagagta gagagggcgg gacgtggact attcagacta
     1621 aaatagagaa gggagagtcg ttgtttccca ggattcagta atggcaaagt ttgcatacga
     1681 cagcacgaaa aatgcagact tattctacgc aataaaggtt ccagtgttgg atcctttctt
     1741 tttcatcgac ttgggtcaag caaaatatgt gtttttagat caccgcgagc ttggcttctt
     1801 ccaggagaaa aacaaaaacc ctaatttgca ggctgtatta ctaaagccca gcggatcttt
     1861 tgaagacttt gcctggcagc ttttagagca atatgggctt gggcaaggcg agctggaagt
     1921 accctttaac tttcctttgc agcttgcgga ctttttgcgc tccaaaggca tagagttatc
     1981 tgtgcaagaa tctttgtatc cagaaagggc aataaaaact acagaagaag tacaggcgat
     2041 ccgccaaagc gtgcaaaaaa ccgagaaagc ctttgcgctt attgagagaa ttttacgaga
     2101 gtctactatt caggaaaaca gtattttgta taacgaggag gttcttacga gcgagtttct
     2161 aaagagtgag gcggaaaaac tgctgctcaa ggaagacatg atttcagaag agggaattat
     2221 catatcctgc gggcagcacg ccgctattcc gcaccatccg ggcgagggag gactaaaacc
     2281 tcaccaaacc atagtttgcg atttgttccc gcgtagccgt accacaggat actttgccga
     2341 cattaccaga acttttgtaa aaggtgcgcc acccgaagaa atacagaaaa tgtatgaggc
     2401 ggtgctctta gcacagcaag aaggtataaa agccgtggcg ccgggtaaaa aggcaagcga
     2461 catccacaaa gtttgcattg atgttttatt gcaggcaggc taccacgtgg gcgacaaagg
     2521 ttttctgcac aacacgggcc acggccttgg tttggaggtg catgaaaacc cgcgactcgg
     2581 ggaaaagtca gaggaaacac tggagcccgg caatgtagaa accattgagc caggattgta
     2641 ttacccggag cgcggaatag gcatccggat cgaggacgac atactggtaa ccgcccaagg
     2701 cgcagaaaat ctagtgagct accccaaagg atgggt
//
LOCUS       DASRUP010000095         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2183344, whole genome shotgun sequence.
ACCESSION   DASRUP010000095 DASRUP010000000
VERSION     DASRUP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183344"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..433
                     /gene="rplS"
                     /locus_tag="VFE94_03735"
     CDS             5..433
                     /gene="rplS"
                     /locus_tag="VFE94_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZX50223.1"
                     /translation="MKQQLPDIQPGDTVRVHQKVKEGPSTGSGQAKERIQVFEGLVLA
                     RKHGKGINATITVRKISQGVAVERIFPLHAPFIQKMEITKRAKVRRAKLYYLREAKGR
                     KARLKAKEFGIEVVEPEAPKAEAEVKEEVPAEETEQKKEV"
     gene            439..522
                     /locus_tag="VFE94_03740"
     tRNA            439..522
                     /locus_tag="VFE94_03740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:gag)
     gene            580..656
                     /locus_tag="VFE94_03745"
     tRNA            580..656
                     /locus_tag="VFE94_03745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Val,seq:tac)
     gene            656..916
                     /locus_tag="VFE94_03750"
     CDS             656..916
                     /locus_tag="VFE94_03750"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HZX50224.1"
                     /translation="MEFVLYFLAGIVQDFLVTLDWRFIAKNKVAPATLLSFLGTVVAM
                     LVLYNILTQLDANRGITAIIVYAAGIAAGTFLGMKFKFGLKD"
     gene            1384..1454
                     /locus_tag="VFE94_03755"
     tRNA            1384..1454
                     /locus_tag="VFE94_03755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1416..1418,aa:Gly,seq:gcc)
     gene            1527..2567
                     /locus_tag="VFE94_03760"
     CDS             1527..2567
                     /locus_tag="VFE94_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZX50225.1"
                     /translation="MPLAITFDDVLLVPQVSDVNPSEIDLRSRLTRHITLATPLISAA
                     MDTVTEAKLAIALAKEGGIGILHRNCTIEEQVKMVAQVKKHDASLPVGAAIGPHDIER
                     AKALDQAGADLVSIDCAHAHKPNIVADAKKIKKAIKADLVVGNIATPEAAKEMVSFAD
                     ALKVGVGPGSICTTRIVSGVGMPQLTAIMDVAKVARAKNVPVIADGGIRFSGDITKAL
                     AAGADTVMLGSLFAGTNEAPGKVVTVQGKKYKVYRGMGSFAAMQKGKATDRYSQKGSE
                     KHVAEGIEAFTPYKGPLAEVVFQLLGGLRSGMGYTGAKNIVELHKQARFVRITQAGRQ
                     ESHPHSITLKND"
     gene            2560..>2949
                     /locus_tag="VFE94_03765"
     CDS             2560..>2949
                     /locus_tag="VFE94_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50226.1"
                     /translation="MTDKETQKLPNAETQYDLFDHLVRIVSSTNGISLQETLELATNS
                     ASIKKKKEAFDLLADFRQHLSQFQQYSEDIEVLINHPVGHILREFLRKFPLPYRDEHT
                     HLTGSLTPEFIYPRLMPLLEGPQKNLYE"
BASE COUNT          849 a          704 c          757 g          639 t
ORIGIN      
        1 aaatatcaaa caacaacttc cagacatcca gcctggtgat acggttcgcg tacaccaaaa
       61 agtaaaagaa ggcccttcga caggctcagg gcaagcaaaa gaacgcatcc aggtgttcga
      121 aggtttggtg ctggccagaa aacatggcaa gggaatcaat gccactatta ccgtgcgaaa
      181 aataagccag ggggtagcgg tagaacgtat tttccctctc catgcgcctt ttattcagaa
      241 aatggagatt accaaacggg caaaagtacg cagggcaaaa ctgtattacc ttagggaggc
      301 caaagggagg aaggcacgct tgaaagcaaa agaatttgga atagaggtag tggagcctga
      361 agctccgaaa gctgaagctg aagtgaaaga agaagtaccc gccgaagaaa ccgaacaaaa
      421 aaaagaagta taacaaatgc ggacgtggcg gaattggtat acgcgcaagc ttgaggtgtt
      481 tgtgggcgca agcccgtgga ggttcaagtc ctctcgtccg cacccttcac ttcgttcagg
      541 gcaagctttt aggtttaccc tgaggagcga agcgacgaag ggcggttagc tcagttggct
      601 agagcgtctc gtttacaccg agaaggtcga gggttcgagc ccttcaccgc ccaccatgga
      661 atttgtgctc tattttttag ctggcatagt gcaggatttt cttgttacgt tagactggag
      721 gtttatcgcc aaaaataaag tggcgcctgc taccctgctt tcctttttgg gaacagtagt
      781 agccatgctc gtactctaca atattcttac ccagcttgac gcgaatcggg gaattactgc
      841 cattatagtg tatgcggcag gaattgcggc tggcacattc ctcggcatga aatttaagtt
      901 tggcttgaag gactaatgac aatccgatgg gttaccaaag cagaatctga aaaactggag
      961 ttcgaaaaca cgtatggcga ggcagtgcag acgtactttc aaaattttta aaagtgtgtt
     1021 aaacaaacac tatgccgcgg tagctcagtg gtagagcagt ggcctaagat ctttgggccc
     1081 tctggcggta acgccagatg aaaaattggg tgaattcggg gaaatcctga gcgcagtcga
     1141 aggacaatcc cgagccaagc tccgacctcg cgtcggggaa ggtgtagaga ctatcccgaa
     1201 agggagtagt tcgacataac gtcggacgaa gcgcccaacc cccgcgatcg ccaaatgcga
     1261 ttttgcgggg tgatgatata gtccgatcct tggagcaatc caagaaaatc gctcggaaga
     1321 gcctcgtgtc gggggttcaa ttccctcccg cggcacactg agcccaccga aggcgggcga
     1381 agtgcgggtg tagcacaatg gtagtgcatc tggttgccaa tcagagtacg agggttcgat
     1441 tcccttcacc cgctcccaaa aacggacgcg ctgttgcaaa acagtgtttt cgtttatata
     1501 atcgaaggaa ctcttctatg accagaattc ctcttgccat tacattcgac gacgtgctgt
     1561 tggttcccca agtatcagac gtaaacccgt ctgaaattga tttgcgctcg cgtttaaccc
     1621 gccacattac gctggccact cccctcatct cggccgctat ggatacggta acagaagcaa
     1681 agctcgccat tgccttggca aaagaaggag gcattggcat tcttcaccga aactgcacca
     1741 ttgaagaaca ggtaaaaatg gtggcacagg taaaaaagca cgacgcaagc ctgcccgtgg
     1801 gagctgccat tggcccccac gatatcgaaa gggcaaaggc tttagaccaa gcgggagccg
     1861 acctcgtctc tattgattgc gctcatgcac acaaaccaaa catagtggca gacgcaaaga
     1921 aaatcaaaaa agcgataaag gcagacctcg tcgtgggaaa tattgccaca ccagaagccg
     1981 caaaggaaat ggtgtctttc gcagatgccc tgaaagtagg cgtaggcccc ggttccattt
     2041 gcaccacaag aatcgtaagc ggcgtaggaa tgccccaact caccgctatt atggacgtgg
     2101 caaaggtggc ccgagcaaaa aatgtgcctg ttatagcaga tgggggcatt cgcttttctg
     2161 gagatattac caaggctctt gccgccggag cagacacggt aatgttgggt tctctgtttg
     2221 ccggcactaa tgaagcgccg ggaaaagtag ttaccgtaca aggaaaaaaa tacaaagttt
     2281 acaggggcat gggctctttt gccgccatgc aaaagggcaa agccaccgac cggtacagcc
     2341 aaaaaggttc ggaaaaacac gtggcagaag gcatagaagc ctttacgcca tacaaaggtc
     2401 ctctggcgga agtagtattc cagctgctcg gagggttgcg ctctggaatg gggtacacag
     2461 gggcaaaaaa cattgttgag ttgcacaagc aggcaaggtt tgtacgcatt acgcaagccg
     2521 ggcgccagga aagccacccg cattctatta ccttaaaaaa tgactgacaa agaaacacaa
     2581 aaacttccaa acgcagaaac gcaatacgat ttgtttgacc atttagtgcg cattgtaagt
     2641 tctaccaacg gcatttcgtt gcaggaaact ttggaacttg ccacgaattc agcttcgata
     2701 aagaaaaaga aagaggcatt cgaccttttg gccgactttc gccaacactt aagccaattt
     2761 caacagtatt cagaagacat tgaagtattg ataaaccatc ccgtggggca catactgcgc
     2821 gagtttttgc gcaaatttcc ccttccctat cgcgacgaac atacccatct caccggctcc
     2881 cttacgccgg agtttattta tccaaggctt atgccccttt tggaagggcc gcaaaaaaat
     2941 ctttacgaa
//
LOCUS       DASRUP010000096         6733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1556900, whole genome shotgun sequence.
ACCESSION   DASRUP010000096 DASRUP010000000
VERSION     DASRUP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556900"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFE94_03770"
     CDS             <1..645
                     /locus_tag="VFE94_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX50227.1"
                     /translation="EQARNNPDFVCGNGGIGCMEEADVMLVLDRSGSIDAGEMTTMKN
                     AAKAFVDALAPSTGGVHVGMVSFSSSATLDQVLTDDGTTVKTAIDNIPSPGSGLTNLE
                     DGIRTAQLELDTNGRAGVAKFMVIITDGEPTASDGPGTNADDAEDAATDSKNAGTEIF
                     AVGVGIGASGAAFLQNDIVSAPSANHYFNATDFDDLEAILEDLVDCPNGQLVSP"
     gene            716..1273
                     /locus_tag="VFE94_03775"
     CDS             716..1273
                     /locus_tag="VFE94_03775"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZX50228.1"
                     /translation="MVKAAGKIVYYGFIGFLVVIALAIAGSVFPITGNYKILVVQSGS
                     MEPAIHTGAVVVVKPSSEYQQGDIITFGPATKKTPPITHRIVNIEGQGRAATYQTKGD
                     ANENADPNPVPSRDVLGKVIFNVPYIGYVIDFARQPIGFALIIGIPALVIIGDEMRKI
                     MVEARRLLKQRHAKADKLPTTEENL"
     gene            1230..2342
                     /locus_tag="VFE94_03780"
     CDS             1230..2342
                     /locus_tag="VFE94_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50229.1"
                     /translation="MQRLINCRQPRKTSKGFGQVAPYLFHHDKVTWEAPSPVHCKVKQ
                     KHSRAKIVVVLLVLGVNLLGMSVITETVAYFNDTETSGGNLLAAALLDFELDSTGDFS
                     PKVSPSHPASRLVSVEDIQSILFHYQVRAENFAGPLCDVLHFKAMLQGDTKYEGPLVS
                     FEAGEFGFGDPDDWGFFASLASLGEEFAGKQCEFDLVYSGWQLPFPLLFSGFHDEERI
                     PSVIIAKEKRHDDEEEDDEDKDDKEDKEDKEDKGEKGDKGDKGPKPEDDEEEEDYKAE
                     TEVEGGGTEEVGHTDENDQGDETAPEPLIEAEPTSTIGDEPPAPTVPSEPEPAVEPLA
                     EETVVEEPAENTEPPVDEPAKEVMPVEVVEEPVDIF"
     gene            2439..3155
                     /locus_tag="VFE94_03785"
     CDS             2439..3155
                     /locus_tag="VFE94_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50230.1"
                     /translation="MKHILSLLVTAGIVSLAVVGVTAKEAFAQDTTATPTVVEEQAAL
                     QEIAQQLQDIEVEVNRLSLLVNKLVLEKQAVALQERLLAEMSSPSPAPVVARPAPVAV
                     APTPAPLAPAPAPIAQAPVPAPTPAPREDNVFGGVTLTPEPEAAAVTRLQDRFMSITS
                     PLRNLGGPELLTLAILAVLGTVVLARRARRRGGAAPLETAETSNQPTGSGSTVVTPQV
                     TQSQSQEPRQGELRRGVAWK"
     gene            3226..3498
                     /locus_tag="VFE94_03790"
     CDS             3226..3498
                     /locus_tag="VFE94_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZX50231.1"
                     /translation="MNKEELLEMIAQKANINKRQAGDALEAFIDGVTATLKKGKSVAL
                     TGFGTFTSSKRAARPGRNPKTGEKIRIPAMTVARFKPGKGLKDAVR"
     gene            3501..4085
                     /locus_tag="VFE94_03795"
     CDS             3501..4085
                     /locus_tag="VFE94_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZX50232.1"
                     /translation="MPAKEFSKNPLEIEGFWFYDCQMPAKEEKLFLEAYEKYGDAIFR
                     HCCFKVSDRELALDLAQETFKRVWLEIARGTQIVNMRAFLYKVANNLAVDEYRKKKVS
                     SLEAATEGGVQFASNKDKDIGKIIDAKTVFSKAEQLDEIYREAVLLRYAEELSVKEIA
                     EIAGESENVISVRIHRGLAQLRIILEKEEYGPET"
     gene            4069..5073
                     /locus_tag="VFE94_03800"
     CDS             4069..5073
                     /locus_tag="VFE94_03800"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HZX50233.1"
                     /translation="MDQKLKEFFQKAKRVQMQKEEKSLGLKALLSYIEENPVRFGDNT
                     RRQVQRSIISDLQRFLQLLYKPMAVALVLLITVAVSGGASLAAENALPGDILYPVKTE
                     VNEGVRSFLSFSAETRTNWKVEQVERRLQEAEELAAKGKFSGKASAELDAKVEQHAEQ
                     LSQMASRFNSQGRADLAAEIHSDLEATFDAHGKVLGLVRGEVNGLELSEFISVVRTRA
                     TTARQARQEAEVEVQAQGGTQQRQAAEGKMSAAENKIAEVKNFIAAKAKTQAETDAEV
                     KLQAAESAFANGSAKLQAGSYGEAFVLFQQAMRVAQEAKAYAQADAVLEIKIEAGLQL
                     "
     gene            complement(5068..5988)
                     /locus_tag="VFE94_03805"
     CDS             complement(5068..5988)
                     /locus_tag="VFE94_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZX50234.1"
                     /translation="MIKIYHKAVREQQLTELADFRVGSLIYVENPTEEELNQLEQRYK
                     ADHSLLLDALDLNEVPRLEHEEGITYVFTRFPIRETNRVVTLPLLIAVGGDFVMLVSP
                     KPLPLLERFLTGKADFYTTQKSKLFTQIFSQLNLGYQGFLREMSRDVRSTTVRFERVR
                     NRDIIQFVAFERVLNDFLAALVPTNRILEDILSGKYFKLYEEDHELTQDVRLANGQLI
                     ESCKSDLKTIVNFREAYSTIMTNNLNRVIKLFTALTVVLTVPTMIASFYGMNVALPFQ
                     NSPMAFWWILWGTIIIAIFILGTFVWKRWL"
     gene            complement(5996..6069)
                     /locus_tag="VFE94_03810"
     tRNA            complement(5996..6069)
                     /locus_tag="VFE94_03810"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6035..6037),aa:Pro,seq:ggg)
     gene            complement(6082..6270)
                     /locus_tag="VFE94_03815"
     CDS             complement(6082..6270)
                     /locus_tag="VFE94_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50235.1"
                     /translation="MAQQCAICGKHSNIAWRLVKLRGKYNPTAKHKQKPNLQWTRLLS
                     GGRVKACTKCIKNLARTS"
     gene            6326..>6733
                     /locus_tag="VFE94_03820"
     CDS             6326..>6733
                     /locus_tag="VFE94_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZX50236.1"
                     /translation="MEFTDIVYLILVLVFSVVLHEVSHGWVAHSQGDPTAKQAGRLTL
                     NPIPHIDPIGSLLVPFFLLLTTQTAPGGPYAFGWAKPVPVNPNNFRDQKFGSAKVSFA
                     GPAANLAIALVFGLLLRFFSGPIAALSPELIGML"
BASE COUNT         1817 a         1522 c         1881 g         1513 t
ORIGIN      
        1 gaacaagcga ggaataatcc agactttgtg tgcggaaatg gaggcattgg ctgcatggaa
       61 gaggcagacg taatgctggt gcttgatcgt tctggcagca tagacgctgg agagatgaca
      121 accatgaaaa acgcggcaaa agcgtttgtc gatgccttgg cgccttcgac aggcggcgtg
      181 catgtgggaa tggtaagctt ttccagctcg gccacgcttg accaggtact tacagacgac
      241 ggcactacgg taaaaaccgc gatcgataac atcccgtcgc ctgggtcagg tttgacgaat
      301 ctagaagacg gaattcgaac tgcacagcta gaattggata ccaacggcag ggccggggta
      361 gctaagttca tggtaattat taccgatggc gagcctaccg caagtgatgg cccaggtacc
      421 aatgcagacg atgcagaaga tgcggcaacc gattccaaaa acgccggcac ggaaatcttt
      481 gcagtagggg taggcattgg tgcgagcggc gctgcgttcc tgcagaacga catcgtgagt
      541 gctccgtctg caaaccacta cttcaatgct acagactttg acgacttgga ggcaattctg
      601 gaagatttgg ttgattgtcc gaatggacag ctagtgagcc cctagtgctg cagattctct
      661 gaagctcttt accctcgctt tctttgagaa ggcgggggga taagggtttt atggcatggt
      721 aaaagcagca gggaaaattg tatattacgg gtttataggg tttttggtgg tgattgcttt
      781 ggcaattgcg ggttcggttt tccccattac cggcaactac aaaattctgg tggtgcagtc
      841 tggttccatg gagccggcca tacatacggg agccgtagta gtggtaaaac cttcgagtga
      901 gtaccagcaa ggcgacatca ttacctttgg cccggcgacc aaaaaaacac cgcccattac
      961 tcaccgcatt gtaaatatcg aggggcaagg gcgggcagcc acataccaaa ccaagggcga
     1021 cgccaacgaa aacgcagacc caaaccctgt gccgtcacgc gacgtgctgg gaaaagttat
     1081 attcaatgta ccctatattg ggtatgttat tgactttgca aggcagccca taggttttgc
     1141 cttaattatt ggaattccgg ctttagtgat tattggcgac gagatgcgca aaattatggt
     1201 cgaggcaagg cgtttgttaa aacaaagaca tgcaaaggct gataaactgc cgacaaccga
     1261 ggaaaacctc taaagggttt gggcaggttg ccccatacct ttttcaccac gacaaagtta
     1321 cgtgggaggc gcccagcccg gtgcattgca aggtaaagca aaaacacagc cgggccaaga
     1381 ttgtggttgt tttgctggtt ttgggagtga acctgctcgg tatgtcggtt attacagaga
     1441 ctgtggcgta ttttaacgac acggaaactt ccggggggaa tttgctggct gccgcgcttt
     1501 tggactttga actggactcg acaggggatt tttcgcccaa agtttcaccc agccaccctg
     1561 cttcccgcct tgtgagcgta gaagatattc agagtatttt gttccattac caggtgcggg
     1621 cagaaaattt tgcagggcct ttatgcgatg tgttgcattt taaggccatg cttcaggggg
     1681 ataccaaata cgaagggcct ttggtaagct ttgaggccgg ggagtttggc tttggggatc
     1741 cagacgactg gggatttttt gcctctttgg caagccttgg cgaggaattt gccggaaagc
     1801 agtgcgagtt tgacctggta tattcagggt ggcagctgcc ctttcccctg ctgttttccg
     1861 gtttccatga cgaagagcgc atcccaagcg ttattatagc taaggagaaa aggcacgacg
     1921 acgaagagga ggatgacgaa gataaggacg ataaagagga caaagaggat aaggaagata
     1981 agggtgaaaa aggcgataaa ggggataagg ggccaaagcc cgaagacgat gaggaagaag
     2041 aggactataa ggcggaaacc gaggtagagg ggggtgggac agaagaggta ggacatacag
     2101 atgaaaatga tcaaggcgac gagacggcgc cagaacctct aatagaagcg gaacctacct
     2161 caacgatagg tgatgagccg cctgctccga cggtaccgtc ggagccagag ccagcggtgg
     2221 aacctttggc agaagaaact gttgttgagg agccagccga aaacacggag ccgcccgttg
     2281 acgaacccgc gaaagaagtt atgccagtag aggtggttga ggaacctgtg gacatttttt
     2341 gacaaatggt gtaagacaaa ggtataaggt cacgaattgc atataaacaa aagaaaggtc
     2401 gaaattaaca cagtaccaac gaatttccag aaacacttat gaaacacata ctttccttat
     2461 tggtaacagc cggaatagta agtttggcgg ttgtaggcgt aaccgccaaa gaagcgtttg
     2521 cgcaagacac tacagcaact ccaacagtcg tagaagaaca ggcagcgttg caggaaatcg
     2581 cccagcagct gcaggatatt gaagtagagg taaacaggct ttctttgctt gtaaataaat
     2641 tggtattaga aaaacaggca gtcgcgcttc aggaacgatt gctggccgaa atgtcgagcc
     2701 cttccccggc tcccgtagta gcgcggccgg ctccggttgc agtggcacca acgccggctc
     2761 ctctggctcc tgcaccagct cccatagccc aggctcctgt accagccccg actcctgcac
     2821 ctagagaaga taatgtgttt ggaggggtta ctcttacccc ggaaccggaa gcagcggcgg
     2881 tgaccaggct gcaagaccgc tttatgagca taaccagccc gttgcggaat ttgggcgggc
     2941 ccgaattgct tactttggca atcctcgcgg tattgggaac agttgttctt gcaagaaggg
     3001 cgcgccgccg cgggggagca gctcccttgg aaacggcaga aacttcaaac cagccaaccg
     3061 ggtctggcag tacggtcgta actccccagg taacacaaag ccagagtcag gaaccgcgac
     3121 aaggcgagtt gcgtagagga gttgcctgga agtaatcctc ttgtagtggt ataatacacc
     3181 tcgattgcaa ggtcggaatt tttatacaac aaacaaacaa tactaatgaa taaagaagaa
     3241 ctcctcgaga tgattgccca gaaggccaac atcaacaagc gacaggcagg agatgcgctt
     3301 gaagccttta tcgacggcgt taccgccact ctcaaaaaag gcaagtcggt agcgctcacg
     3361 ggttttggca cttttacgtc ttccaagagg gccgcgcgcc cgggaagaaa ccccaaaacc
     3421 ggcgagaaaa tccgcattcc cgcaatgact gttgctcggt ttaagcccgg aaaaggtttg
     3481 aaagacgcgg tgcgataaag ttgccagcaa aagaattttc caaaaaccct cttgaaatcg
     3541 agggtttttg gttctatgat tgccaaatgc cggcaaaaga agagaagctg tttttggaag
     3601 catacgaaaa gtatggcgac gcgatatttc gccactgctg ctttaaggta tccgacaggg
     3661 agcttgcttt ggatttggcg caggagacct ttaagcgcgt atggctggag attgccagag
     3721 gaacgcaaat tgtcaatatg cgggcctttc tgtataaggt tgcaaacaac ctggcggtcg
     3781 acgaataccg gaaaaagaaa gtttcttcgc tggaagctgc aaccgaaggc ggcgttcagt
     3841 ttgcctcgaa caaggataaa gatataggta agattataga tgccaaaacc gtcttctcta
     3901 aagcggaaca gctcgacgaa atatacaggg aagccgtgct gttgcggtat gccgaggaac
     3961 tctcggtaaa agagatagcc gagattgcgg gggagtcgga aaatgtaatt tcggtgcgca
     4021 ttcaccgcgg cttagcgcag ctgcgaatta tattagaaaa agaagaatat ggaccagaaa
     4081 cttaaagaat tttttcaaaa ggcaaaacgg gtacagatgc agaaagaaga gaaaagcctt
     4141 ggcttaaagg ccttgttgag ttatattgaa gaaaatcctg taagatttgg cgacaacacc
     4201 cgtcgtcaag tgcaaaggtc aattataagc gatttgcaaa ggtttttaca acttttatat
     4261 aaacctatgg cagtagcatt agtacttctc ataacagtcg cggtgagcgg aggggcttcg
     4321 ctcgcagcag aaaacgcttt accgggggat attctttacc cggtaaaaac cgaggtgaat
     4381 gagggagttc gttctttctt atcgttctcc gctgaaacca ggacaaactg gaaagtggag
     4441 caggtagaac gcaggctcca agaggcagaa gaactcgccg cgaaaggcaa gttttcagga
     4501 aaagcctcgg cagagctcga cgcaaaagtt gaacagcatg cagagcagtt gtcgcaaatg
     4561 gcatcgcggt ttaactccca ggggagagcc gatttggcgg cagagattca ttcggatctc
     4621 gaggcaacgt tcgatgccca cggaaaagtt cttggcctgg taaggggcga ggtaaacggc
     4681 ctggaattaa gcgagtttat ctctgttgtc agaacgcgag ctaccactgc aaggcaggcg
     4741 cgtcaggaag cggaagttga agttcaggcg cagggaggaa cacagcaaag gcaggcggct
     4801 gaaggaaaga tgagcgccgc ggagaataaa attgccgaag taaaaaactt tatcgcggca
     4861 aaagctaaaa cccaggctga aaccgacgcc gaggtaaaac ttcaggcagc agagagcgcg
     4921 tttgcaaacg gaagcgcgaa attgcaggcg gggagttatg gtgaagcatt tgtactgttt
     4981 cagcaggcaa tgcgagtagc acaagaagca aaggcgtatg cccaggctga cgccgttctt
     5041 gaaataaaga ttgaagcagg cctgcaatta tagccagcgt ttccagacaa aggttcccag
     5101 aatgaaaatg gcaataataa tggtgcccca gagaatccac caaaaggcca taggcgagtt
     5161 ttggaagggc agggcaacgt tcatgccgta gaagcttgca atcatggtgg gaaccgtgag
     5221 caccaccgta agcgcagtaa acagcttaat aacgcggttt aagttgttgg tcattatggt
     5281 ggagtaggct tcacggaaat tcacaatggt tttcaagtcc gatttgcacg actcaataag
     5341 ctggccgttg gcaaggcgca cgtcttgcgt gagttcgtgg tcttcttcgt agagcttaaa
     5401 gtatttcccg ctcaaaatgt cttccaaaat ccggttggtg ggaaccagcg cagcaaggaa
     5461 gtcgttgagt acgcgttcga atgcaacaaa ctggataata tcgcggttcc gaacgcgctc
     5521 aaagcgcacc gtggtgctgc gcacgtcgcg gctcatttcg cgcaaaaacc cctgataacc
     5581 aaggttgagc tgcgaaaaaa tctgcgtgaa gagtttggat ttctgagtgg tataaaaatc
     5641 cgcctttccg gtgagaaagc gctccaaaag gggaagaggc tttggtgaaa ccagcattac
     5701 aaagtcgccc cccacggcga tgagtaaggg cagcgtaaca acgcggtttg tctcgcgtat
     5761 cgggaaccgg gtaaatacat aggttatgcc ttcttcgtgc tctaagcggg gcacctcgtt
     5821 gaggtctaaa gcgtcgagta aaagactgtg gtctgctttg tagcgctgct ctagctggtt
     5881 gagctcttcc tcggtagggt tttccacata aattaaagag ccaacacgaa aatcagcaag
     5941 ttcggtgagt tgctgttccc gcactgcctt gtgatagatt ttgatcatgc gctgttggtc
     6001 gggctgccgg gaattgaacc cgggtcgcac ccaccccaag ggtgtatact gccgttgtac
     6061 tacagcccgg gcaccccaaa cttagcttgt tctcgcaaga ttcttgatgc acttggtaca
     6121 cgcttttacg cgcccgccag aaagaagcct cgtccactgg aggttcggtt tttgtttgtg
     6181 cttggccgta ggattatatt ttccgcggag ctttaccagg cgccaggcga tattgctgtg
     6241 ttttccgcaa attgcgcatt gttgagccat ttgccaacta tagcaaagtt tgaaaaatgt
     6301 ttcaagttat gctaacgtaa agactatgga atttacggat attgtgtatc tcattctggt
     6361 gctggtgttt tcggtggtgc ttcacgaagt gtcgcacggg tgggtagcgc attcccaagg
     6421 cgaccccacc gcaaagcaag cagggcgcct tacattaaac cctattcctc atatcgaccc
     6481 catcggctct ttgctggtgc cgttttttct tttactaacg acccaaaccg cacctggagg
     6541 gccttatgct tttggctggg caaaaccggt tccggtgaac cccaacaatt tccgcgatca
     6601 gaagtttggt tccgccaaag taagctttgc gggccctgcg gcaaaccttg ccattgccct
     6661 tgtgttcggg cttttgctgc ggtttttcag cggccccatt gctgccctgt ccccagagct
     6721 cattggcatg ctg
//
LOCUS       DASRUP010000097         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2187881, whole genome shotgun sequence.
ACCESSION   DASRUP010000097 DASRUP010000000
VERSION     DASRUP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187881"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFE94_03825"
     CDS             <1..391
                     /locus_tag="VFE94_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZX50237.1"
                     /translation="LALRQLGKLDRFNAHRKEIAQKYYTALTGSTFELPKQFAERENI
                     FLRFVVKHKNAHEIIRKAWTKNLLIGDWYTSPLAPDDTQLNKMRYEKGVCPKAENLAQ
                     VTLNLPTHINIREKELQKIIVFIKDAG"
     gene            381..1406
                     /locus_tag="VFE94_03830"
     CDS             381..1406
                     /locus_tag="VFE94_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HZX50238.1"
                     /translation="MQVREITDKHIWENFLLRCPQRTFFQSWNWGDFRQASREKIWRF
                     GVYDREQLIAVALTSKKVARRGTYLEIAHGPSLVEVVHRKEALETLLERLREMARQEG
                     ASFVRINPLWEGSPQNNAVLRNLGFRGAPMYTTYESTWKLDITPAEEELLKNMRKAAR
                     YLIRQNLKNEDITIRQGLETGDLEEFHKLSRAVGERQKFIPFSKKDTEQEIAALQKDN
                     QVSLIFGLYKGKPVAAALAVFWAGIGFYHQGASLGEYAKLSVPYLVQWEAIREAKRRG
                     CALYDFWGFVDPQKYPKHPWAGPTLFKMGFGGQAFEYVKTQDYPVSWKYWPTWIFETT
                     RKKRRRL"
     gene            1676..2398
                     /locus_tag="VFE94_03835"
     CDS             1676..2398
                     /locus_tag="VFE94_03835"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZX50239.1"
                     /translation="MFQIKHIEVQGAQKVPAEKLQELTREHIPKKFAFLSINNLFLAN
                     VGRVAQEVQAAFPEIETVAVDTTFPDKIKVTIKERGAVAVWCQQKNFTVEVSEGEEQA
                     ARSVRECFAMDSGGVIFEQKEPEGEVVIFGGKSEAVLGEQVLAQELLSNILLFQKQMD
                     AAALFQEVGLRVSGITVFSEDRANARISEGWEIYLNPADNIDWQTTKAKLVLDQEIPF
                     AKRPLLEYIDLRFGDQAYIKYR"
     gene            complement(2399..>2833)
                     /locus_tag="VFE94_03840"
     CDS             complement(2399..>2833)
                     /locus_tag="VFE94_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50240.1"
                     /translation="YIGSSLMAFPNYIPYYNALAGGTENGYKIAVDSNYDWGQDFYRL
                     LDFVEANNIQKVYLDYFGGEDPAYWLGNRYAEMRCGDPNRQQTCARAKQEKGWVAVSA
                     NQLMGGTASPVTGFDQPTGYYAWLLDFEPTRLGTSIFIYNIK"
BASE COUNT          813 a          578 c          750 g          692 t
ORIGIN      
        1 tttggcgttg cgccagctag gaaagctcga taggtttaac gcccaccgaa aagaaatcgc
       61 gcaaaagtat tatacagctt taactggcag cacgtttgaa ttgccaaagc aatttgcaga
      121 acgcgaaaac atttttttgc gttttgttgt aaagcacaaa aatgcccatg aaattatcag
      181 aaaggcgtgg acaaaaaact tgttgatagg ggattggtat accagccctt tggcgcccga
      241 tgatacccag cttaacaaaa tgcgatatga aaagggcgtg tgccccaagg cagaaaatct
      301 tgcccaagta acccttaatc tgcctacgca tataaacatt cgggaaaagg agctgcaaaa
      361 aattattgtg tttataaaag atgcaggtta gagaaataac cgacaagcat atatgggaga
      421 attttttgtt gcggtgcccc cagagaactt ttttccagtc gtggaattgg ggtgatttta
      481 ggcaagcctc cagagaaaag atatggcgtt ttggggtata cgatagggag cagttaattg
      541 ctgttgctct aacatccaaa aaagttgcca gaagaggaac gtacctggag atagcgcacg
      601 gcccctctct ggtcgaggta gtccatagaa aggaagcact cgaaacgctc ttagaaaggc
      661 ttcgcgaaat ggcgaggcaa gaaggggcaa gctttgttcg gataaatcct ttgtgggaag
      721 gaagtccgca gaataatgcg gtgctaagga acttgggctt caggggagcg cctatgtaca
      781 ccacatacga atcgacgtgg aaactcgata tcacgcctgc agaagaggaa ctcctcaaaa
      841 atatgcgaaa agcagccagg tatcttatcc gtcaaaacct taagaacgaa gacattacca
      901 tacggcaagg tttggagacc ggggatttgg aagaatttca taagttaagc cgtgcggtag
      961 gggagcgcca aaagtttatt cccttttcaa agaaagatac ggagcaagaa atagctgcgt
     1021 tgcaaaaaga caatcaggtc tccctgattt tcgggttgta taagggcaaa ccggtagctg
     1081 ctgcgttagc ggtattttgg gcaggaatag gattttatca tcaaggcgct tcgctcggcg
     1141 agtacgcaaa gctttcggtg ccgtatctgg tgcaatggga agcaatccgc gaagcaaaaa
     1201 ggcggggatg tgcgttgtac gacttttggg gatttgtaga cccccaaaaa tatcccaagc
     1261 atccatgggc aggaccaacc ttatttaaaa tggggtttgg ggggcaggca tttgaatatg
     1321 taaaaacgca ggattatcct gtatcatgga aatactggcc cacgtggatt tttgaaacta
     1381 cgcgaaaaaa acgaaggcgt ctataaacgg ctacgagaga cgaaattttg gaaatcgaga
     1441 cccctacccc tcgctctgct cggggccacg ccctaaccct acgatttcaa aatttgtctc
     1501 tcttcgccct tagaaacgtt ttttatttat gccatcaaag cgatataagc cgattcggcg
     1561 gttaaaacca aagcggtctc cctggaaaaa ggtcttaaaa agccggtttt tttggtttag
     1621 tctgttgggg gttttcactt tgggagggct ggggtacgga ttatttttta ctcccgtgtt
     1681 tcaaataaag cacattgagg tacaaggagc gcaaaaagtg cccgcggaaa aactgcagga
     1741 acttaccaga gagcatatcc caaaaaagtt tgcttttttg agcataaata atttgtttct
     1801 ggcaaatgtg ggcagggtgg cgcaggaagt gcaggccgcg tttcccgaaa tagaaacggt
     1861 agcggttgat accacgttcc cagacaaaat aaaggttacc ataaaagaac gcggggcagt
     1921 ggcggtgtgg tgccagcaga aaaactttac ggtagaggtt tcggaaggag aagagcaggc
     1981 agcgcggtcg gttcgagagt gctttgccat ggacagtggt ggcgttattt ttgagcaaaa
     2041 agaacccgaa ggggaagtcg ttattttcgg gggcaagagc gaagccgtgc tgggcgagca
     2101 ggtgttggcg caggaactgc tttcaaacat acttttgttc caaaaacaaa tggacgctgc
     2161 tgcgcttttt caggaagttg gtttgcgtgt ctctggcatt accgtgtttt ctgaagaccg
     2221 ggcaaatgca agaatttctg aaggctggga gatatatttg aatcctgcag acaatataga
     2281 ctggcaaacg acaaaagcca aactcgtgtt ggaccaggaa atcccgtttg caaagcgccc
     2341 cttacttgaa tacatcgatt tgcgctttgg cgaccaggcg tatattaagt atcgctaact
     2401 atttgatatt gtaaataaaa atggaggtgc cgaggcgggt gggttcaaaa tccaaaagcc
     2461 aggcgtagta gccggtgggc tggtcaaatc ccgtgacagg tgaggcagtg cctcccatga
     2521 gttggtttgc cgaaacagca acccagcctt tttcttgttt tgcccgcgcg catgtttgtt
     2581 gccggtttgg atctccgcag cgcatttcag cataacgatt gcccagccag taggcggggt
     2641 cttcgccgcc aaaatagtca aggtaaactt tttgtatatt gtttgcttcc acaaaatcga
     2701 gtaggcggta aaaatcctgg ccccagtcgt agttggaatc cacggcaatc ttgtaaccgt
     2761 tttctgtgcc gccggccaaa gcattgtagt aaggaatata attgggaaac gccataagag
     2821 aagatcctat ata
//
LOCUS       DASRUP010000098         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1065413, whole genome shotgun sequence.
ACCESSION   DASRUP010000098 DASRUP010000000
VERSION     DASRUP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065413"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFE94_03845"
     CDS             <1..350
                     /locus_tag="VFE94_03845"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HZX50241.1"
                     /translation="TGHCDPSGPGGPVGPVGPTLPSITNIQFKNPLKVGSLTELINRL
                     IDFIFTVSLIVAPILLVIAGVVFMTAAGDSSRVGTARRMLMWTIVGFGIILISKGLVE
                     VLRGILGVTGPAP"
     gene            355..696
                     /locus_tag="VFE94_03850"
     CDS             355..696
                     /locus_tag="VFE94_03850"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50242.1"
                     /translation="MKYKFFFGFLFSIALFFVLVPLNTEAQGVATVPQYSAQEVFDIL
                     KTIGNWIFVAALLLVPIMLIVGASFVLTGGDNPSRFATGRNVFIGTIVGLLLVLLARA
                     VFAAIQNIFGN"
     gene            798..1154
                     /locus_tag="VFE94_03855"
     CDS             798..1154
                     /locus_tag="VFE94_03855"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50243.1"
                     /translation="MKKIFAAVLLCLVLTVAVAGVAFAQTVPAAPRTIPSGITTATGF
                     IALLNDLTDWLFVILVVIAVVFIVVAGLQFIMAQGDPTAISKARMSLIWAAVGIGVAL
                     LARGLPEAVENLLTGQ"
     gene            1233..1511
                     /locus_tag="VFE94_03860"
     CDS             1233..1511
                     /locus_tag="VFE94_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50244.1"
                     /translation="MPTGFLQQAPTGPTTGEDLIILVETLTNWLFVGFMLVALLFILF
                     AAAKFIAQGGDPRAIGQERNKLMWAAVAIGIALLARAVVPAIGWIVGL"
     gene            1525..1917
                     /locus_tag="VFE94_03865"
     CDS             1525..1917
                     /locus_tag="VFE94_03865"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50245.1"
                     /translation="MKKLFAILFLFCILTAVAGEAVLAQTPANPPRNPPIRFFGGVIP
                     SGGVQSGDDFLDIMAAILNWIFVGVMLIAVLMILVAAFQLITAGGDAQSVAQARNKLL
                     WGAVGVGIALLAWAVPNAISGLFGQNLP"
     gene            1920..2903
                     /locus_tag="VFE94_03870"
     CDS             1920..2903
                     /locus_tag="VFE94_03870"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZX50246.1"
                     /translation="MKKLLLRPEAFVYFTLAVFGSIFAALFFGYNQAFASDFLSYTLG
                     RDMQKVMWIFAAFGTALAVHFAVFRRRFSGLSPFFRSLLFMGIPVLLSFFVLVAAVGQ
                     LNTLNSTRLKDQLLFQWDTFLTGTFLPLSAGSVQYPSWFVQAVAFSYTFLVPAFVLLA
                     LYLLRVNQKLFREAAAVFSIGSLLLLAGWVMFPVLSPHDRFIDNVYQLPIPPAIQQYV
                     GAYSPQAEIQSFLAGMRDLKEKLEFLPTSTFPSGHAAWGVWLVYFAWRVRPKLLFAVV
                     PLVTLSSVGTFLFAQHYFVDVPAGIAVATLSVFVVRWALRARAETPAPQRA"
BASE COUNT          580 a          663 c          801 g          909 t
ORIGIN      
        1 ccactggaca ctgcgatccg tcggggccgg gcgggccggt ggggccggtg gggccgacgc
       61 tgccttcgat taccaatatc cagtttaaaa atcctttgaa ggtagggtcg cttacggaat
      121 taattaaccg gctcatagac tttatcttta cggtttcgct tattgtggct cccatactgc
      181 ttgtaatagc tggcgtcgta tttatgaccg cggccggcga ttcgagccgc gttggcactg
      241 cccggcgcat gcttatgtgg accattgtgg gctttggcat catcttaatt tccaaagggc
      301 ttgtggaagt gctgcgaggc attttgggcg taacaggccc cgccccttaa tcccatgaaa
      361 tataaattct tttttggatt tctattctcc attgctttgt tctttgtttt ggtgcctttg
      421 aataccgagg cacagggagt agctaccgtt ccccaatatt cggcgcagga ggtgtttgac
      481 attctcaaaa ccatcggcaa ctggattttt gtggcagcgc tcttactcgt gcccattatg
      541 cttatcgttg gagcttcttt tgttttgacc ggaggcgaca accccagccg ctttgcaacg
      601 ggcagaaacg tttttatcgg gacgattgtc gggcttttgc tggtgttgct tgccagggcc
      661 gtgttcgcgg ccattcaaaa tatctttggc aactagcacg ctggggaaaa cttgcttgtg
      721 taagctgtaa ggttaaggta taataaggca taagaaaatt cggaaaggtc gaagaaattt
      781 ttacgatggt atagtatatg aaaaaaatat tcgcagcagt tttgctttgc ctcgttctca
      841 cagtagcggt tgcgggagta gctttcgcac agacagtgcc tgcagcgccg agaaccattc
      901 cttcgggcat tactacggca acgggattca ttgctcttct gaacgacctt accgactggc
      961 ttttcgtgat tttggtggta atcgcagtgg tatttattgt tgtagccggt ttgcaattca
     1021 ttatggcgca gggagatccg accgccatat caaaagcgcg catgagcctt atctgggctg
     1081 cagtaggtat cggtgtggcg ttgttagcaa gaggactgcc ggaagcagtg gaaaaccttc
     1141 ttacgggcca gtagaaaaca aactttttcc cccgcgcatt gcgcggggtt ttctttggca
     1201 aaggttttgg tataattgaa atagtcttgc ttatgcctac tggattctta caacaggcgc
     1261 ccacggggcc aacaacagga gaagatctga ttattcttgt ggaaacgctc acgaactggt
     1321 tgtttgtggg ttttatgctg gtcgcgctgc tttttattct gtttgcagcg gcaaaattta
     1381 ttgcgcaggg gggagatccg cgagcaatcg ggcaggagcg caacaaactc atgtgggcag
     1441 cggtggcaat tggaattgct ttactggcga gggcggtagt gcccgcaatt ggctggattg
     1501 tcgggttgta gtataataga taatatgaaa aaactttttg cgattttatt tcttttttgc
     1561 atacttaccg cggttgcggg agaagccgta cttgctcaaa cgccagctaa ccctcctaga
     1621 aaccctccta taagattttt cgggggagtt attccttcgg gcggcgtgca atctggggac
     1681 gactttctcg atattatggc cgctattctc aactggattt ttgtgggggt aatgcttatt
     1741 gccgttctca tgattttggt tgccgccttt cagcttatta ccgccggggg cgatgcccaa
     1801 tcggtggccc aggcaaggaa taaacttttg tggggggcgg ttggtgttgg catagctttg
     1861 ttggcttggg ctgtccccaa cgcaatttct ggccttttcg gacagaatct tccataacta
     1921 tgaaaaagct gctgcttcgc ccggaagcct tcgtatattt tacattggct gtatttggga
     1981 gcatttttgc cgcgctcttt ttcggctaca accaggcttt tgcttcagac tttttgagct
     2041 atactctggg ccgcgacatg caaaaagtaa tgtggatatt tgcggctttt ggcacagcgc
     2101 ttgcggttca ctttgcggtg tttaggcggc gcttttcggg tttatctccg ttttttcgaa
     2161 gtctgctttt tatgggtatt cctgtcttgt taagcttttt tgttttggtg gctgcagttg
     2221 ggcagttgaa tacgctcaac agcacgcgtc tgaaagacca gcttttgttc cagtgggata
     2281 cgtttttaac aggcaccttt ttgccgctat ctgcgggttc tgtgcagtat ccttcgtggt
     2341 ttgtgcaggc ggtcgcgttt tcttacacgt ttctggtgcc ggcatttgtg ttgcttgctt
     2401 tgtatttgct tcgggtaaac caaaagcttt ttcgggaagc cgccgcagtg ttctcgatag
     2461 gatctctttt gctgctggcc gggtgggtga tgtttccggt tctctcgccg catgaccggt
     2521 ttatagataa cgtgtaccag ttgcccattc cccctgccat ccagcagtat gttggcgcgt
     2581 attctcccca agcagaaatt caaagttttt tggcagggat gcgggactta aaagaaaagc
     2641 tcgagttttt gccaacgagt acgttcccca gcgggcacgc agcgtgggga gtgtggctgg
     2701 tgtattttgc ctggcgcgtg cggcccaaac ttctctttgc cgttgttccg cttgttacgc
     2761 tttcttcggt aggcacattt ctgtttgcgc agcattactt tgttgatgtg ccagccggaa
     2821 tcgccgtggc aacactttct gtgtttgttg tgcgttgggc cttgcgggcg cgcgcggaaa
     2881 cgccggcacc ccaaagagct tgacatacaa cagatttgtg ataatatata ctgcatatcc
     2941 gaatgcagtt ttt
//
LOCUS       DASRUP010000099         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_429110, whole genome shotgun sequence.
ACCESSION   DASRUP010000099 DASRUP010000000
VERSION     DASRUP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429110"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /gene="topA"
                     /locus_tag="VFE94_03875"
     CDS             complement(<1..1374)
                     /gene="topA"
                     /locus_tag="VFE94_03875"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZX50247.1"
                     /translation="MKRLLIVESPTKARTLQSFLGKDYNVQSSFGHVRDLPKSKLGVD
                     VDKNFEPSYVIPTKARKTVSALKKEAKKADSVILASDEDREGESIAWHLAQALGLQDP
                     ERIVFHEITKQAIQEALEHPRKIDNNLVEAQQGRRILDRLVGYKLSPFLWKKVARRLS
                     AGRVQSVAVRLVVDREREIEKFKPDEYWSVEALFEKEKQQFKAALSAKDGKAVEKLDI
                     HNKEEADAILQDLEGAEYKIAQIEKKETNKNSLPPFTTSTLQRAAASRFHFPARLTMS
                     VAQQLYEQGHITYHRTDSLNLAQSALAAAQEFITKEYGTNYYAGKPRVFKTKSKGAQE
                     AHEAIRPTVPANRPDSLKLTPQQTKLYKLIWQRFIASQMSPAVFDATSIDIAAANYTF
                     HATGQMLKFAGFLKVYPIKFEETDLPGLEKDEILDLKKLLSEQHFTQPPPRYNEASLI
                     KALESYGI"
     gene            1531..2082
                     /locus_tag="VFE94_03880"
     CDS             1531..2082
                     /locus_tag="VFE94_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50248.1"
                     /translation="MSIETVALPSGVIEALKEMFAFFVNHPVDSPQTRTKVVEFCQIV
                     LQNGVSMEEASEESFASVLLEGFGETWDVLVSRLPYLSEDQKNALQNDMGNLFETWEK
                     CSLYTRKEIDGAAKKIHEEVIDVRRHFGLDSIPVDVVPEKTLEVFVHDLLDDTFGGLL
                     KNLPLVERTRKELMIQEKIRAFY"
BASE COUNT          583 a          497 c          565 g          717 t
ORIGIN      
        1 aatgccataa ctttccaaag ccttaattaa gcttgcttcg ttgtagcgtg gaggcggctg
       61 ggtaaagtgt tgttccgata aaagcttttt taagtcgagt atttcgtctt tttccaaacc
      121 tggaagatcg gtttcctcga attttatggg gtatactttt aagaacccgg cgaatttgag
      181 catctggccc gttgcatgga atgtataatt tgcggccgca atatcaatgc tggtggcgtc
      241 gaacacggct gggctcattt gcgaggcgat aaacctttgc caaataagtt tgtacagctt
      301 tgtttgctgg ggcgtaagtt ttaaagaatc tggcctattt gctggaaccg tgggacggat
      361 tgcttcgtgc gcttcctggg cgccttttga ttttgtctta aacacgcgcg gttttcccgc
      421 gtaatagttt gtcccgtatt ctttggtaat aaattcttgt gccgcggcaa gcgccgattg
      481 ggcaaggttt aacgaatctg tccggtggta ggtaatatgc ccttgttcat ataactgctg
      541 ggcaacactc atggtaagcc gggccgggaa atgaaacctg gaggccgcgg cgcgctgcaa
      601 cgtagaagtg gtaaacggcg gcaaagagtt tttgtttgtt tcttttttct caatctgggc
      661 tatcttatat tctgcccctt ccaagtcttg taaaatggcg tcggcttctt ctttgttgtg
      721 gatatccagc ttttcaacgg ctttgccgtc ttttgcagag agcgctgctt taaactgttg
      781 cttttctttt tcgaacaatg cttcaacgct ccagtactcg tcgggtttaa acttttctat
      841 ttcgcgctcc ctatctacca ccaaacgcac ggctaccgac tgcacgcgtc cggcagaaag
      901 cctacgcgcc acctttttcc aaaggaaagg cgacagcttg tatccaacca gacggtctaa
      961 aattctcctt ccctgctggg cctcaaccaa attgttatca attttgcggg ggtgttccaa
     1021 cgcttcttga atagcttgtt tggtaatttc gtggaaaaca attcgttcgg ggtcttgcaa
     1081 acccagggct tgggccaggt gccaggcaat actttcgcct tcgcggtctt catcggaagc
     1141 aaggattacg gagtctgcct tttttgcttc tttttttagg gcagatacgg tcttgcgggc
     1201 tttcgtggga atcacatagc ttggctcaaa gtttttgtcc acgtcaacgc ccagtttgct
     1261 ctttggcaaa tcgcgaacat gcccaaaaga agactgcacg ttgtagtctt ttcctaagaa
     1321 ggactgcagg gtccttgctt ttgttggact ctccactatt agaagtcgtt tcatacaaaa
     1381 tcaaactata ctcgaatatc aaaaatttgt ccagtttatg ataaaactgt tcttacttta
     1441 ccacagagtt ctcgcaaaac aaaaacttta cttttaagac aatttttgat atagggattt
     1501 tagctaatac tagacagaga ggtctgtgtg atgagtattg aaacggtagc gttgccaagt
     1561 ggtgttatcg aggccctaaa ggagatgttt gctttcttcg tgaaccatcc agtagatagc
     1621 ccgcaaacgc gaacaaaagt agtagaattc tgccaaatcg tgcttcaaaa tggcgtaagc
     1681 atggaagagg cttcggagga gagttttgca agcgttctat tggagggctt tggggagaca
     1741 tgggatgttt tagtctcgcg gcttccttat ctttccgaag accaaaagaa tgctctgcaa
     1801 aatgatatgg gcaatctttt tgaaacttgg gagaaatgtt cgttgtacac acgaaaagag
     1861 atagacggtg cagcaaagaa gattcacgaa gaagtgattg acgttcggag gcattttggt
     1921 ttagatagta tccctgtgga tgttgtgcca gaaaagacac tagaagtctt cgtgcatgat
     1981 ctacttgatg atacatttgg agggcttctc aagaatcttc cattggttga aagaaccaga
     2041 aaagagttga tgatccaaga gaaaataaga gcattctact aggttaggac tttgtagggt
     2101 tggtgctatc gcgccaaccc tcttaatttt tcaaccgact ctgacaacat ttggcagtag
     2161 aggttgaggc caatggtgtt tacggtgctg gatgagtcta ccgaaggttt agcctttctt
     2221 gcactacggc aataaattct tcgatatcaa ccaaacgatt tttccctgcg tcgtacacaa
     2281 tgccaaaatc cacatcttcg taatcgtggg cgataatgtt ccggaatttt gccatattga
     2341 taagcttgtc agaaagctct tt
//
LOCUS       DASRUP010000100         8494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_260411, whole genome shotgun sequence.
ACCESSION   DASRUP010000100 DASRUP010000000
VERSION     DASRUP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260411"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFE94_03885"
     CDS             <1..490
                     /locus_tag="VFE94_03885"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50249.1"
                     /translation="MGVLQKQATSFDQMLLYEQVAENAALQESWQAHVESMRAFKQFA
                     NEQGAELLFVIIPWKFQVYDFFLASEFPDAAERARVIAEPSRLLQEALGQEGIHSLDL
                     LPLFRQQANLEPRKYLSGQEDLFFRFDLHWSPNGQLLAGLLTAEYIKNKALLETQEAN
                     AP"
     gene            487..678
                     /locus_tag="VFE94_03890"
     CDS             487..678
                     /locus_tag="VFE94_03890"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HZX50250.1"
                     /translation="MKFLYSFVQTVALKSVVTRNLFVFLWHMKMWWAMPVIVLLLVFF
                     LLILFAQSSPLGPLIYTIF"
     gene            680..907
                     /locus_tag="VFE94_03895"
     CDS             680..907
                     /locus_tag="VFE94_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50251.1"
                     /translation="MREKLHYLWRGFQKVSLFVGNIISTVFMIAFYFSLFALVALPAR
                     FFGKYLAQKGLYSNWVLRGQEKNSLQDFQHE"
     gene            900..2690
                     /locus_tag="VFE94_03900"
     CDS             900..2690
                     /locus_tag="VFE94_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HZX50252.1"
                     /translation="MSRILGLSFFYHDAASALVVNGVPVAMAEEERFSRKKHDAGFPS
                     NAISFVLQQGGIKPEELDWVVFYEKPFLKFDRIFKTSLSTFPFAPLYFSHGLKNVFTS
                     KLWIRETIAKKVGVSQKKILFAEHHLSHAASAFFASPFEQSAILTIDGVGEWACATIG
                     KGEGNNITILKEIHFPHSLGLLYSAFTAFLGFEVNEGEYKVMGMAPYGKPKYADKVRE
                     LVHFFDDGSFSLNLKYFAFHHSTETTFSKAFCDLFGKPRERQSQFFTRATGWPSYFGP
                     RPDEETYRAMAQQQEYYADIAASVQQVVEEGIIRLARHAKEQTGMENLCYAGGVALNS
                     VANWKVAQQTSFREVFIQPAAGDGGGALGAALVADAMVEKNERKFCQQHALYGKSYGK
                     EEIQAELENTSRVSWSYVEDEQELCEKVAAELAMGRVAGWFQGRFEWGPRALGARSIV
                     ADPRREAMKDLVNTKIKFREPYRPFAPSVLAEHAAEFFELAGREHEYPFRFMLYVVPV
                     KKEKEGVVPAITHVDGTARPQLVHKDTLPLYWKLIHAFYEKTGVPMVLNTSFNLKGEP
                     IVNTPANALSTFQKSGLDLLVLGNFLVYKS"
     gene            2738..4012
                     /locus_tag="VFE94_03905"
     CDS             2738..4012
                     /locus_tag="VFE94_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HZX50253.1"
                     /translation="MKIWNIYVDSARAPRYIKRLATAGLWNPLLYPLYLLSHLAPRRA
                     DLVVCGLPRKFVWNSKYVFLYANNSGQKDLKVVWISRSKPIVRELQDKGYPAYYWLSW
                     QGMWYPLRAKYHVIDASIETINYWLSGGAKVVLIWHGIPLKKIARDVDKGLSLDIVLH
                     RTSGFKNTLFRYLLPWRFRQTDYIVATSPVFADISSSAFRVAKERIFVGGFPKNDLLF
                     HEVPGSEIGADRPMLQKLEEHRRTGRKTVLYAPTWRDSGGDSFFEKKEDLQALDGFLE
                     QNGLVFFLKLHPLAQEKAFRAAHNGGYKNIFFVRPDSDADPLLPLTDILVTDYSGIYF
                     EFLLLDRPVVFFAFDYEKYVSQDRELYFPYGEVTPGPKAKNLRELMGYLAEVSAGRDD
                     YAEHRKKVRDLAYTWHDGNSAQRVCEFLKQLG"
     gene            4017..5183
                     /locus_tag="VFE94_03910"
     CDS             4017..5183
                     /locus_tag="VFE94_03910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX50254.1"
                     /translation="METPKKKVLFLIPTLAAGGAERVVSELSKHAPDHIELVLVVFEK
                     KVSYEFRGRVESLAVPVTSPILPRFPRFFLRWWRLRKLLTKEKPDSVVSFGYSADLLN
                     ILCNRKAVIRVDMFLSAGRRGVFGSFTKLIAKVLFRRAALILAVAHAIKEDLIQAFGV
                     PEQRIRVIPNPIDMQRIQQLAREPLPVEYQHIFGKPVVVTMGRLTRQKGQWHLLKAFG
                     YIKSKAPEAQLVVLGEGVLKDQLRALAADLGIEKQVHFLGWQENPYRFLGKARVFALS
                     SLWEGLPMVLLEAMACGLPVVSTDCKSGPKEILDPDIGILTPVCARDWGTMGKEISRE
                     EQLLGQALLRVLGDKEYAALLAQKSLSRAQDFDNSRVLGQYDFLWKEMFYEKRL"
     gene            5180..6169
                     /locus_tag="VFE94_03915"
     CDS             5180..6169
                     /locus_tag="VFE94_03915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZX50255.1"
                     /translation="MKTISWIELSSSLQGGFAYNEQARAALAKEYTVENANVQPRVLR
                     KFRPLKFFEIMARLLFLQGKSDLWVRNFYAVLTFPFDRTQGKHIAVRHHADFSGFPWW
                     SQPFFMLQEKLFEHNAKRLDALVVVSAYWKNHFEKKGFSNVRVIYNGFDLARFSPSQE
                     EVAAFQKKYGLEGKPVVYLGNCQKAKGVVESWKALQSLNVHLVTSGKRMVRIPAKNLD
                     LPYREYLCLLACCNVVVAMSQFKEGWNRTAHEAMLLRRPVVGSGLGGMKELLEGGEQP
                     VCQDFSKLKDMVEYLLSHKEEGEKLGNLGYEFAKTFSRERFEKEWFSLVRTLL"
     gene            6166..7584
                     /locus_tag="VFE94_03920"
     CDS             6166..7584
                     /locus_tag="VFE94_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50256.1"
                     /translation="MTTFIAAFFVLLVFSGATWFLLGRYAKKHPYAKRIFLFALCVHA
                     VAAFGIYYTEFYPFGMGDQERYQTAAELVASDFRAGVFSLDRISSHLYITSAAHWYPV
                     VLGSVYAVSVPDRLVGMVLNAWIAALSILIVFFLALEISKSSRGAFWAGVVASLYPAH
                     VYFGSLLLKDATVVLLALAGLLFAVKIMQRFSPRYVIAFGIALALLFHFRFYIGVALL
                     YAFSFSWPFIRGFTWKQKVQYGTFTLLSLGLIPSFLGYGYYALDIVAHYTRIDQIELY
                     RESAYIFENSPDVQVGENQGQGVEGSTIVVEEEVAGSKPEAAGLASVQGEEQSAPKGA
                     GGSTVIVKVGDGPLSFIRNYLVSLSFVSLGPFPWHFSLPQHYFAFVEVIPWWILLFFI
                     VLGVRKRWREWQVFLPLLLFALGTLVMLALLIDNFGIYMRLRMPVFLVLLAFLAVPLS
                     RMSKNQLYEIIRYWRRRFHRLA"
     gene            7538..>8494
                     /locus_tag="VFE94_03925"
     CDS             7538..>8494
                     /locus_tag="VFE94_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZX50257.1"
                     /translation="MKLFVTGGGGFIGSHRGAIKICFVTTADLTLRFILFDQMEFLKQ
                     QGFEVWGVCSAGKWAGELEQRGFRVATVRMTRQLFTPLADARALWELVRLFRQEKFDI
                     VHTHTPKASFLGQIAAFVAGVPIRIYTIHGLFFLKDSSWRRKLAFGTVEKILSFLVNK
                     ALFVNREDMEEVQRMNIYGSNKITYIGADVDLSRFDPSLHSRDEIQKAKSELGIPLDK
                     VVIGIVARLVWEKGFSDLFSAMQIILQKFPHAVLLVVGPSEPEKGDGFQPSVVETYGI
                     QENVVFAGERTDVPTMYALMDVFVLPSYREGLGLSALEASAME"
BASE COUNT         2035 a         1830 c         2252 g         2377 t
ORIGIN      
        1 catgggagtt ttgcaaaagc aggccacctc ttttgaccag atgctcttat acgaacaggt
       61 tgcggaaaac gcggccctcc aggaaagttg gcaggctcat gtggagagca tgagggcttt
      121 caagcaattt gcgaatgaac aaggtgcaga gcttttgttt gtgatcattc cctggaaatt
      181 ccaggtgtac gattttttcc tggcaagcga gtttccggat gccgccgaaa gggcacgcgt
      241 tattgcagaa cccagccggc tgctgcagga agctcttggg caagaaggaa tccattcttt
      301 ggaccttctt cctttgtttc gccaacaagc gaatttagag cctcggaagt atctttcagg
      361 gcaagaagat ttgtttttca ggttcgacct gcactggagc cccaacgggc agctacttgc
      421 aggattgctc accgcagagt atattaaaaa caaggcactc ttagaaaccc aggaagcaaa
      481 tgcgccatga aatttttata ttcgtttgtc cagacagtcg cacttaaaag tgtggttacg
      541 cgtaaccttt ttgttttcct gtggcacatg aaaatgtggt gggcaatgcc cgttattgtg
      601 ctcttgcttg ttttctttct tctcattctg tttgcccaaa gcagccccct tggcccattg
      661 atttacacca ttttctagta tgagagagaa actgcactat ctctggcgtg gtttccagaa
      721 agtctctttg tttgtgggaa acattatttc caccgtgttt atgattgctt tttatttttc
      781 cctttttgcg cttgttgcac tgcccgcacg attttttggc aagtatcttg cacaaaaggg
      841 tctgtattcc aactgggttt tacgagggca ggaaaaaaat tcgctgcagg actttcagca
      901 tgagtagaat tttgggcctc tctttttttt accacgatgc cgcctcggct cttgtcgtta
      961 acggagttcc ggttgccatg gcagaagagg aacgtttttc ccgcaaaaag cacgatgccg
     1021 gttttccctc aaatgcaatc tcgtttgtgc tccaacaggg cggaataaag ccagaagagc
     1081 tggactgggt ggtattttac gaaaaacctt ttctcaagtt cgatcgcatt ttcaagacaa
     1141 gcctttctac gtttccgttt gctcctttgt acttttcgca cgggttaaaa aacgtattta
     1201 cttcgaagtt gtggatcagg gagaccattg ccaaaaaagt tggcgtttcg caaaagaaaa
     1261 ttctgttcgc agaacaccat ctttcgcatg cagcttccgc gttttttgct tctccgtttg
     1321 aacagtcggc tattcttacg atagatggag tgggcgaatg ggcatgcgcc accattggca
     1381 agggagaggg aaacaatata accattctca aagaaattca ttttcctcat tctctgggtc
     1441 ttttgtattc cgcctttacc gcgttccttg gcttcgaggt aaatgaaggg gaatacaaag
     1501 taatgggcat ggctccttac ggaaagccaa aatatgcaga taaggtgcgg gaactcgtgc
     1561 attttttcga cgacggatcc tttagcttaa acctcaaata ttttgccttt caccattcta
     1621 ccgagactac cttttccaaa gcattttgcg atttgtttgg aaagccccgc gagcgccagt
     1681 cccaattttt taccagggct accggctggc cttcgtattt tggcccgcgg ccagacgaag
     1741 aaacgtatag ggcaatggcg cagcaacagg aatattatgc agacattgcc gcttcggttc
     1801 agcaggtggt ggaagaagga attatccgtt tggcccgcca cgccaaagag cagacaggca
     1861 tggaaaacct ttgttatgca gggggcgtgg cattaaattc ggtagccaac tggaaagtgg
     1921 cacagcaaac atcgtttcgg gaagtgttta ttcagcctgc ggccggcgac ggagggggag
     1981 cgttgggtgc ggcgcttgta gcagacgcaa tggtagaaaa aaacgagcgc aagttctgcc
     2041 agcagcatgc gctttacgga aaatcatacg gcaaagaaga aattcaggca gaactagaaa
     2101 acacttccag ggtttcgtgg agttatgtgg aagatgaaca agagctgtgt gagaaggtag
     2161 ccgcggaatt ggcaatgggc cgggtagcag ggtggtttca gggaaggttt gaatgggggc
     2221 cgagagcatt gggcgcgcgt tcgattgtgg cagacccccg tagggaagcc atgaaagatt
     2281 tggtgaatac caaaatcaaa ttccgggaac cctaccggcc tttcgctcct tcggtgcttg
     2341 cagaacacgc cgccgaattt tttgagctgg cgggcaggga acacgagtac cccttccgct
     2401 ttatgctgta cgtagtgccg gtgaaaaagg agaaggaggg cgtggtgcct gcaataaccc
     2461 atgtagatgg aactgcgagg ccccagctcg tacacaaaga tacactccct ctctattgga
     2521 aacttatcca tgcgttttat gagaaaacgg gagtacctat ggttttgaat acatctttta
     2581 atctgaaggg tgagcctatc gtgaataccc cagcaaacgc gctatccacg tttcaaaaaa
     2641 gcggtttgga tttgcttgta ttaggcaatt ttcttgtcta taaatcctga atatgtcaag
     2701 ttgcgaaagg gttcaaagca aggtatgata aagcccaatg aagatctgga atatctacgt
     2761 ggattccgcg cgcgcgccgc ggtatataaa gcgtttggcc accgccggct tgtggaaccc
     2821 cttgctgtat cctttgtacc ttctttcgca tcttgctccc cgaagggcag atctcgtagt
     2881 atgcgggttg cccagaaaat tcgtgtggaa cagcaagtat gtttttttat acgccaataa
     2941 cagcgggcag aaggatctta aagtggtatg gatttcgcgg agtaagccaa ttgtgcgaga
     3001 gctccaagat aagggatatc ctgcatacta ctggttgtcg tggcagggaa tgtggtatcc
     3061 tttgcgggca aagtaccatg taatagacgc ctctatcgaa accattaatt attggttatc
     3121 tggaggagcg aaagtggtgc ttatttggca cggcatacct ctgaagaaaa ttgcgcgcga
     3181 cgtggacaaa gggctttctt tagacattgt gctccaccgc accagcggat ttaaaaatac
     3241 tcttttccga taccttttgc cctggaggtt tcgccagacg gattatattg ttgccacttc
     3301 tcctgttttt gccgacattt cttcttcggc gttccgcgtt gccaaggaac gcatttttgt
     3361 tggcggattt cctaaaaacg atttgctgtt ccacgaagtt ccgggatctg aaattggggc
     3421 agacaggcca atgctgcaaa agttggagga gcatagacga actgggcgca aaaccgtgct
     3481 gtatgcccct acttggcgcg acagcggggg agactctttt tttgaaaaga aagaagattt
     3541 gcaggcgctt gatggttttt tggagcagaa tggccttgta ttctttttaa agctgcaccc
     3601 tttggcgcag gaaaaagcat tccgcgcggc gcataacggc ggctacaaaa atatcttttt
     3661 tgtacggccg gattcagatg ccgacccttt gctgccttta acagacattt tggtaaccga
     3721 ctactctggc atttattttg aatttttgct tttggaccgc ccggtggttt tctttgcgtt
     3781 cgactatgaa aaatatgtaa gccaggaccg cgagctttac tttccttatg gggaagttac
     3841 gccaggcccc aaagcaaaaa acctgcgaga actcatgggc tatctcgcag aagtttctgc
     3901 aggcagggac gactacgcag aacaccgaaa aaaagtacgc gatttagcct atacctggca
     3961 cgacggaaat tctgcgcagc gcgtgtgcga gtttctcaaa caactaggat aaaggcatgg
     4021 aaaccccaaa gaaaaaagtc ttgtttctta ttcctacgct tgctgcgggc ggcgcagaac
     4081 gggtagtgtc tgaactttcc aagcacgctc cagaccatat tgagctggta cttgtagtgt
     4141 ttgaaaaaaa ggtttcttat gagtttcggg gcagagtaga gtctttggcg gtgcctgtaa
     4201 cgagccctat acttccccga ttcccccggt tttttctccg atggtggcgg ctgcgaaaac
     4261 tcctcacgaa agagaagccg gattctgtcg taagctttgg atattctgcc gacctgctca
     4321 acattctttg caacagaaaa gcggtaatcc gcgtggacat gtttctttct gcaggcagga
     4381 ggggagtgtt tggaagtttt accaagctta ttgcaaaagt tctttttcgg cgagccgctc
     4441 taatccttgc ggtagcccat gcgatcaaag aggatttaat acaggcattt ggcgtgccag
     4501 agcagagaat tcgcgtgatt cccaacccca ttgatatgca gagaattcaa cagctcgcca
     4561 gagagccgct tccggtggaa taccagcaca tatttggaaa gcctgtggtg gttaccatgg
     4621 gtaggcttac ccgccaaaaa ggccagtggc atttgttaaa agcatttgga tatataaaaa
     4681 gcaaagcgcc cgaagcccaa ctggtggtgc tgggagaagg cgtcttgaaa gaccagcttc
     4741 gcgccttagc cgccgacttg ggcatagaaa aacaagtgca ttttttgggt tggcaggaaa
     4801 atccgtatcg gtttttgggc aaagctcggg tgtttgcgct ttcttctttg tgggaagggt
     4861 tgcccatggt gcttttggaa gccatggcat gcggcttgcc ggtggtttcc accgactgca
     4921 agtcggggcc aaaagaaatt ttggatcccg atattggcat tcttaccccc gtatgcgcga
     4981 gggattgggg tactatggga aaggaaattt ccagggaaga acagttgctt ggccaagcac
     5041 ttcttcgggt gcttggagat aaagagtacg cagccttgct tgcgcaaaaa tcgctttcga
     5101 gagcccaaga ctttgacaat tccagggttc ttggccagta tgatttctta tggaaagaaa
     5161 tgttttatga aaagcgctta tgaagacaat ttcctggatt gaactttcct ctagtctgca
     5221 gggcgggttt gcgtacaacg agcaggcgcg tgctgcgctt gcaaaagagt atacggtgga
     5281 gaacgccaat gttcaaccaa gggtgctgcg aaaattcagg cccttgaagt tttttgaaat
     5341 aatggcgcgt ttgctcttct tgcaaggcaa aagcgatttg tgggtgcgga atttttatgc
     5401 ggtacttacg tttcctttcg atcgtaccca gggcaaacac attgccgtgc gccatcacgc
     5461 agatttctct ggctttccat ggtggagcca gcccttcttt atgctgcaag aaaagctgtt
     5521 tgaacataac gcaaaaaggc tcgacgccct tgtggtggtt tctgcgtact ggaaaaatca
     5581 ttttgagaaa aaaggatttt cgaatgtgcg cgtgatttat aacggatttg atttggcccg
     5641 gttttctcca agccaagaag aggtggctgc tttccagaag aagtatgggc tcgaaggaaa
     5701 acctgtggtg tatctgggaa actgccagaa agcaaaagga gtggtggagt cgtggaaagc
     5761 cttgcagagt ctcaacgttc atttggtaac ttcgggcaag cgcatggtgc gcattccggc
     5821 aaaaaacctg gatttgcctt accgagagta tctttgcctt cttgcatgtt gtaacgtcgt
     5881 cgtggcaatg tctcaattca aagaagggtg gaacagaact gcacatgaag ctatgttgct
     5941 gcgccggccg gtggtgggat cgggtttggg tggcatgaaa gagcttttgg aagggggaga
     6001 gcagcctgtg tgccaggact tttcaaaact gaaagatatg gtagaatatc tcctatctca
     6061 caaggaggag ggagaaaagc ttggaaacct gggatacgag ttcgcgaaaa cgttttctcg
     6121 ggaacgattt gaaaaagaat ggttttcgct tgtacgtact cttttatgac tacttttatt
     6181 gccgcatttt tcgtattatt ggtattttcc ggagccacct ggtttttgct cggcagatac
     6241 gccaaaaagc acccatatgc aaagcgtatt tttttatttg ccctatgcgt gcatgctgtt
     6301 gcagcctttg gaatttacta tacggagttc tatccttttg gaatgggcga tcaggaacgg
     6361 tatcagacgg ccgcggaatt ggttgcctct gattttcggg cgggcgtttt ttccctcgat
     6421 cgcatttcct cgcatctcta cataaccagc gcagcccact ggtacccggt agtgctggga
     6481 agtgtatatg ccgtatcggt gcctgatcga ctggtgggga tggtgttgaa cgcatggatt
     6541 gcggcactgt ccatactcat tgtattcttt cttgctttgg agataagcaa atcttcccgg
     6601 ggcgcctttt gggcgggggt tgtcgcaagc ttgtatccgg cccatgtgta ttttggcagt
     6661 ttgcttttga aggatgccac ggtagtattg ctggcgctgg cagggctgct tttcgccgta
     6721 aaaataatgc agcgtttctc gccaagatat gttattgctt ttggaattgc ccttgctctc
     6781 ctgtttcact ttcgttttta tataggtgta gctctcctgt atgcattctc tttttcatgg
     6841 ccttttatcc gcggatttac ctggaagcaa aaggtgcagt atggtacgtt tacgcttttg
     6901 tcgctgggat tgatcccgtc gtttttgggg tatggctact atgcccttga tatcgttgcg
     6961 cactatacgc gcattgatca aattgagctg tatcgagaat ccgcatatat ctttgagaat
     7021 tctccagatg ttcaagtagg cgaaaatcaa ggacaaggag tagagggatc tactatcgtg
     7081 gtcgaagagg aggttgcagg cagcaagccg gaagcagcgg gcctggcgtc tgttcagggc
     7141 gaagagcagt ctgcgccaaa aggggcgggg ggatccactg tcattgttaa agtgggagac
     7201 ggtcccttga gttttattcg aaactacctc gtttccttga gttttgtgtc gctggggcct
     7261 ttcccgtggc atttttccct accccagcat tatttcgctt ttgtagaggt cataccctgg
     7321 tggattcttc ttttcttcat tgtgttggga gtacggaaac gatggcgtga gtggcaagtg
     7381 tttctgcctt tgctcttgtt tgctttggga acgctggtaa tgcttgccct gttgatcgac
     7441 aactttggaa tatatatgcg gcttcgcatg ccggtatttc tggtattgct tgcttttctc
     7501 gcagttcccc tttctcgtat gagcaaaaat caattatatg aaattattcg ttactggagg
     7561 aggcggtttc atcggctcgc ataggggggc tataaaaatc tgttttgtca ctaccgcgga
     7621 tttgaccctt cggttcattt tgtttgatca aatggagttc ttgaaacagc aggggtttga
     7681 agtgtggggg gtttgctctg cggggaaatg ggcgggggag cttgaacaga ggggatttcg
     7741 cgtggcaacc gtgcgcatga cacggcagct ttttacgcct ctggcagacg cgagggctct
     7801 atgggagctt gtgcggttgt tcaggcaaga aaagttcgac atcgtacaca cgcatactcc
     7861 caaagcaagc tttcttgggc aaatcgccgc atttgtggca ggtgtcccta ttcgtattta
     7921 caccattcat gggcttttct ttttaaaaga ctcttcatgg aggagaaaac tcgctttcgg
     7981 aacagttgaa aagattttat catttttggt gaacaaggcg ttgttcgtga atagagagga
     8041 tatggaagaa gtgcaaagga tgaatatcta cggctctaac aagattactt atataggagc
     8101 cgatgtggac ctttctcgtt tcgatcccag tctgcactcg agagatgaaa tccaaaaagc
     8161 aaaaagcgaa ctcggaattc ctttagacaa ggttgtaata ggcattgtgg ccaggctggt
     8221 gtgggaaaag gggttttccg atttgttttc tgcaatgcag attatcctgc aaaaattccc
     8281 gcacgccgtg ttattagtag ttggcccttc agagccagaa aaaggggacg gttttcagcc
     8341 ctcggtagtg gaaacgtacg gtattcaaga aaatgtggtt tttgcagggg agagaaccga
     8401 tgtgccaaca atgtacgccc ttatggatgt atttgttctt ccttcttata gagaggggct
     8461 tggcctttcg gcactggaag cctcggcaat ggaa
//
LOCUS       DASRUP010000101        13003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_751833, whole genome shotgun sequence.
ACCESSION   DASRUP010000101 DASRUP010000000
VERSION     DASRUP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751833"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFE94_03930"
     CDS             complement(<1..571)
                     /locus_tag="VFE94_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50258.1"
                     /translation="MPLEHLFAKLTQAHALSHAYIFSGNDHERKQALLAEIGGKLGWK
                     EFQHPDLFVVQAEGAEAGAEITIAQIRQLYNHLSMGAWSAPYKVAICKDAHRMNREAQ
                     SAFLKLLEEPKGDTVFFLLTPHPEMLFDTIRSRAQEFRFYRFAPPEIKSELKAEFLKL
                     QKASLHDRFDYAKKLADNAEQIPETLQNWL"
     gene            complement(572..1579)
                     /locus_tag="VFE94_03935"
     CDS             complement(572..1579)
                     /locus_tag="VFE94_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZX50259.1"
                     /translation="MFITKIGIDLGTCNSLVFVPKKGVVLNEPSVVAVSILDNRILAV
                     GEEAKEMTGRTPDTIQVYRPLRDGVIADYRVTLAMIRYFLRRVIPRYQFLRPEVLVSV
                     PAGITSTERRAVIQATQRAGVKAAFVAKEPILAAIGAGIPINSSSGHMIVNIGGGTTE
                     VAVISLGGIVVATSLRVAGDKTDQAISEYIKKKHNLAIGETTAEELKMRIGSALLEKQ
                     ETFMEIRGRSLLQGLPKNIKISSNEIAQAVQEPIDEIIYTIKTVLRETPPELSADIMD
                     KGMIIAGGGALLRNIDQRISQAIGVPCFVADDPLTCVARGTGIVLDTLDIYKKSIMSK
                     K"
     gene            complement(1583..2860)
                     /gene="murA"
                     /locus_tag="VFE94_03940"
     CDS             complement(1583..2860)
                     /gene="murA"
                     /locus_tag="VFE94_03940"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZX50260.1"
                     /translation="MEKFVIQGGRPLKGQIDAKGAKNAAFPILAATILTKQDCVVENL
                     PLIEDVYRMLEILEGMGAKIEWVRERAVKVNTEVLDPTKVKKDIVARFRGSVVLLGPL
                     LARFGKVSLPKPGGDIIGARPIDTHLDAFSQLGVKILASNGKVVLEKPESLPDTTVLL
                     NEFSVTATENMMLFAALHPGTITIKTADQDYPIQELTKALSQMGAHITLSGAHAMEVR
                     GKRELAGMKHRLMFDPIEAGTFLIMAAAAKGEVVVKNVETRFLELFLKRLKEFGVPLE
                     VVQEHDGLADLKVLPWKKLKIDKIQTFIYPGIHTDLQSPLGVLATQATGSTLLHDPLY
                     PGRLRYLEELNKMGAEIYIADPHRAIINGPTRLFGTDLGTFDLRGGAAMIIAALIAKG
                     KSEISNIYQVDRGYEKIEDRLSQLGANITRKSA"
     gene            2932..4365
                     /locus_tag="VFE94_03945"
     CDS             2932..4365
                     /locus_tag="VFE94_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50261.1"
                     /translation="MTKRSRTLLFLVFGALFVLAAPAIILFAQGYRFDWNTKHIAKVG
                     AFYFAVTPGQVNVLVNGEFVGTTAPLRENIFISNLLPQTYNVEIQKTGFQTWQKNLLL
                     APKQVTEAKHIILFPEHTSFRALQENVAEMWLAPNQNQAVIKKPISRTLWELSLWDME
                     RNTVYPLYVAQSSRDDILEIIWAADSAKFLMRVVSQEQVKTFVQHIDRQGLSGLLSRQ
                     VALQIGARDRVPLAPLANEIVDIAFAPFSDQQVLYTKPSGNVFVLSLADYLQGQEMQQ
                     IASSMITFGLQGQSIFWLDDKGTVWKKTSLNAEATPINSSPLPMRAETSYELYAFGDE
                     ILIKEFQKLYRLERETQTFKEMFLSAGTIVENPDRQKVAIANGSEIWILFLADQAEQP
                     IHQKGETVFLTRFSAPVENLGWITSWHLLFSVQDTVKITEIDERDRPVTMNIGQFAAP
                     QLFWQNTPATISVVNNGTVSVSETLIP"
     gene            complement(4417..4848)
                     /gene="rpsI"
                     /locus_tag="VFE94_03950"
     CDS             complement(4417..4848)
                     /gene="rpsI"
                     /locus_tag="VFE94_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZX50262.1"
                     /translation="MPRTAQTKTEQQKPSIFYEAVGRRKTAVARVRLFESAKQDFTVN
                     AKPLALHVQSTEYQKTAREALEKGVAGKTFSVTAKVSGGGPHAQAEAIRQGIARALVK
                     FDADLRTNLKTLGFLTRDSRMRERKKFGLKRARRARQWRKR"
     gene            complement(4852..5199)
                     /gene="rplM"
                     /locus_tag="VFE94_03955"
     CDS             complement(4852..5199)
                     /gene="rplM"
                     /locus_tag="VFE94_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZX50263.1"
                     /translation="MNRTTHTIDAKNQRLGRVATQIAILLRGKHKPDFEPYKDMGDFV
                     VVTNVRELAFSGNKMSQKIYYRHSGYLGGLKEKKLGTLFAERPAQVLRKAVWGMLPNN
                     KLRSKQITRLKIS"
     gene            complement(5196..5546)
                     /gene="rplQ"
                     /locus_tag="VFE94_03960"
     CDS             complement(5196..5546)
                     /gene="rplQ"
                     /locus_tag="VFE94_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZX50264.1"
                     /translation="MKKRKAGRIFSRSKGAREGLMLSLVRALALKERIETTEAKAKET
                     SPLFERLVTKSKRDTLATRRELLRYFGPRLTKKMVEEIGPRYKDRPGGYTRITKTMFR
                     KSDGAKMAIIELIK"
     gene            complement(5553..6344)
                     /gene="rpoA"
                     /locus_tag="VFE94_03965"
     CDS             complement(5553..6344)
                     /gene="rpoA"
                     /locus_tag="VFE94_03965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZX50265.1"
                     /translation="MIPLSQSTKIVESKNNRGIFVIEDLYPGYGVTVGNSLRRVLLSS
                     LEGAAVTKVKIKGVDHEFSTLPGLMEDIVSVLLQLKQLRFHMHAEEPQKATLKVKGEK
                     EITGADFELPTQLELLNQDLVLAHLTEKSAELELEIQVEKGVGYVPAEEQNKEKQDIG
                     TIVLDAIFTPIKRVSSRVENMRVGDRTDFNRVTIEVETDGTQSPEQAFFDATDILLKE
                     FQIVQDGVKGALEAQKTVRQKEEPKEDAEKPKKAAKKSVKKTVKK"
     gene            complement(6349..6873)
                     /gene="rpsD"
                     /locus_tag="VFE94_03970"
     CDS             complement(6349..6873)
                     /gene="rpsD"
                     /locus_tag="VFE94_03970"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZX50266.1"
                     /translation="MQKGVVRKRRTSPLSEYGRQLREKQELKEQYNLRERQFSQYIAD
                     TLAKGSTGNAEELFMQALEKRLDNVVFRMGMAQTRKQGRQLVAHGHFFVNGRVVDIPS
                     YRVKRGDVIEIRPQSAAGTLFQNVKLALKKYQSPSWIQLDKANMKGEIMGEPGLEEIA
                     PTVQLPLVFEFYSR"
     gene            complement(6885..7316)
                     /gene="rpsK"
                     /locus_tag="VFE94_03975"
     CDS             complement(6885..7316)
                     /gene="rpsK"
                     /locus_tag="VFE94_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZX50267.1"
                     /translation="MGKKRIIKKGEEAPAPEAKQIGPQSSSKKVREGRVYVYSSYNNI
                     IMTLTDRTGRVLAQVSAGAIGFKGTKKSTPFAASKVADALTQIAKNKGVEELEVFLKG
                     VGAGRDSALRALGAKGFAILSIEDTTPVPHNGPRAAKVRRV"
     gene            complement(7321..7710)
                     /gene="rpsM"
                     /locus_tag="VFE94_03980"
     CDS             complement(7321..7710)
                     /gene="rpsM"
                     /locus_tag="VFE94_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZX50268.1"
                     /translation="MPRLVGINIPDNKQIGIALTYIYGIGPALSKKILAAARIDPIKR
                     ASQLKTEELNALKEVIEKNYRIEGELRRDIMMNIRRLHAIGAWRGVRHAKHLPVRGQR
                     TRTNNRTVRGNVRKTVTSGRKPAASPT"
     gene            complement(7718..7834)
                     /gene="rpmJ"
                     /locus_tag="VFE94_03985"
     CDS             complement(7718..7834)
                     /gene="rpmJ"
                     /locus_tag="VFE94_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZX50269.1"
                     /translation="MRVQSSVKKICKDCKIAKRAGIIYVTCKKNPKHKQRQG"
     gene            complement(7839..8063)
                     /gene="infA"
                     /locus_tag="VFE94_03990"
     CDS             complement(7839..8063)
                     /gene="infA"
                     /locus_tag="VFE94_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZX50270.1"
                     /translation="MIDGNQSEMRKTGEVLEALPSLQFRVKLDDGAEVLAYVSGKLRM
                     HKIRILPGDKVTIEMTPYDTRRGRIVYRGK"
     gene            complement(8089..9276)
                     /locus_tag="VFE94_03995"
     CDS             complement(8089..9276)
                     /locus_tag="VFE94_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50271.1"
                     /translation="MKKKLIDIFPPQSREEKLADAELFEKKQAKPSLASKAPTVWVRF
                     FVLAILLSVVPVILLHFMFARADIKVWPQITRLEINERITVEVQTFEEEKEMTRVFPA
                     TGTQEKDDRAHGSIRVYNTSSGASQTLVASTRFISEEGRLFRSERQAIVPRSGYVDVD
                     VVAAESGQEYNVEASKFSLPGLAGTALYTAIYGESFQPMKGGTQTHVAVVTREDVESA
                     REQLIEALKVQATQSLLGRIPGNVQVVADSVTSVVLQDGALVNEGAELTQFNYSAKVK
                     VSALGFQKNEADLLAKRIVQDSLDEGQSVNEQTLEIAYKVDQPTANAGALSIEVMIHV
                     DQYDALDMQDLKGKLQGVKKNGFTEVFRQYPYLAKAEFSFWPFWVEHVPQSAERIHLD
                     LAF"
     gene            complement(9278..10411)
                     /locus_tag="VFE94_04000"
     CDS             complement(9278..10411)
                     /locus_tag="VFE94_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50272.1"
                     /translation="MGFWSPQRQKSGGSVLAVALDGDTLNFLVFRQMADKKQVLKYGN
                     QQVTAGGLQESLGQVIAQLPREVSVGTLIATFSPDHFKAKSIEIVFPGRGARHVLDKK
                     EALWIEKTMRQQAKAKLFGNLFGESGILPADFRMLRLVPTSQKIDGYSVPRLEGFKAG
                     EIRASFLATFLLATTFFDLAEFVESKKLHTVRVRHMAEAIADFGRKTSREGVYLYVGR
                     RHSQLVVWKQGVLLLPETSLALGEEDFVEVFGEVLGMRDNIAFEFEKRYVEGALSTQV
                     KEKIDSLLLPTFQKFVTLIKKQLEVMKTPLPGNIWIFGRGIKTHHFQEVLQAQLAKGL
                     PFTEDINVHILAPKDIWEGVSFAQNHNPQYSSLCLVADSVLVA"
     gene            complement(10538..12337)
                     /locus_tag="VFE94_04005"
     CDS             complement(10538..12337)
                     /locus_tag="VFE94_04005"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZX50273.1"
                     /translation="MTSNEIRDAYLKFFEEKEHKVIPSAALVPENDPTTLFTGSGMQP
                     LIPYLLGQKHPAGTRLADSQKCFRAEDIDEVGDNRHTTFFEMLGNWSLGDYFKEEQLH
                     WFFEFLTQKVGLPKDRLWVTCFEGDEKNNIPKDEESAAIWKKLGIPKDHILFYGAKKN
                     WWSRAGVPENMPAGEPGGPDSEVFFEFTDVQHDTKFGEQCHPNCDCGRFLEIGNSVFM
                     EYQKQQDETFAKLSQRNVDFGGGLERIAAASNDNSDIFSIDTLQSIIARIEQLSGKKY
                     EGDPKYAFRVIADHIRGAVFMIGDGVLPSNTDRGYFVRRLLRRAVRYADVLGMEQGKF
                     AELSGPVIENYKNQYPELELHRTLIIETIQKEENRFRETLANGLKEFEKLSDNNISGR
                     EAFNLYQSYGFPLEVTLDLAREKGTTVDENGFREELKKHREISRAGAEKKFKGGLADT
                     KEETVRLHTAHHLLLAALRQVLGDHVHQRGSNITAERLRIDFSHPAKVTPEELKQIED
                     LVNQKIAENLDMIRKEMPKEEAMQMGAEMEFGVKYGDVVSVYVAQDIQGNAFSKEFCG
                     GPHVEHTAELGHFKITKEESVSSGIRRLRAILE"
     gene            complement(12361..>13003)
                     /gene="mnmA"
                     /locus_tag="VFE94_04010"
                     /gene_synonym="trmU"
     CDS             complement(12361..>13003)
                     /gene="mnmA"
                     /locus_tag="VFE94_04010"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZX50274.1"
                     /translation="GSIFMAKDQNKDQSYFLSFIKPEVLDKVMFPIGDYTKPEVRKLA
                     KKFGLPNAERKDSQGICFVGKVDFVDFLKEYIPVRQGNIVDTQGNILGQHEGAVYYTI
                     GQRKGLGLAGGPYFVVDKDIKKNTVTVSKNEDHLLQKEVRLKNLNWFVEPKQDLVHAR
                     IRYRQKPAAGTFRIGKSVTAGKLIFDSPQRAITSGQMAVLYQGDQMIAGGVIE"
BASE COUNT         3034 a         3325 c         2937 g         3707 t
ORIGIN      
        1 ccagccaatt ctgcaaggtt tcgggaattt gttctgcatt gtccgccagt ttttttgcgt
       61 agtcaaagcg gtcgtgcaga gaagcctttt gaagcttgag aaattccgcc ttgagctctg
      121 acttaatttc gggcggcgca aaccggtaaa atctaaattc ctgcgcgcgg gaacgaatag
      181 tgtcgaaaag catttcggga tgcggggtta gcaaaaaaaa taccgtatcc ccctttggtt
      241 cttcaagcag ttttaaaaaa gcagattgcg cctcgcggtt catgcggtga gcgtctttac
      301 atattgccac tttatacggc gccgaccagg ctcccatact caaatggttg tagagttgcc
      361 ggatttgcgc gatggtaatt tcggctccgg cttctgcgcc ttccgcctgc acaacaaaaa
      421 gatcgggatg ctgaaactct ttccaaccca gtttgccgcc gatttctgcg aggagcgcct
      481 gttttctttc gtgatcgttg cccgagaata tgtaggcgtg cgaaagcgcg tgggcttgcg
      541 tgagtttggc gaataggtgt tctagaggca tttacttttt gctcataatg cttttcttgt
      601 agatatcgag cgtatccaac acaatgcctg ttcctcttgc cacgcaggtc aaaggatcgt
      661 ctgccacaaa acagggcacg ccgattgcct gggaaatgcg ctggtcgata ttacgcagca
      721 aggctccccc gcccgcaata atcattcctt tatccatgat gtcggccgat agttcgggag
      781 gcgtttcgcg aagcaccgtt tttatagtgt aaataatttc gtcgatgggt tcttgcactg
      841 cctgggcgat ttcgttggag gaaatcttta tgtttttggg cagtccttgc aagagggagc
      901 gcccgcgaat ttccataaag gtttcctgct tttctaaaag cgcgcttcca atgcgcattt
      961 ttaattcttc ggcggttgtt tcaccgatag ccagattgtg tttcttttta atatactccg
     1021 aaattgcctg gtcggttttg tcgccggcca cccgcagaga agttgccacc acaatgcctc
     1081 cgagggaaat taccgccact tcggtggtgc ccccgccgat atttacgatc atgtggcccg
     1141 aagaggaatt aatgggaata ccagccccga tggcggctaa tattggttct ttcgccacaa
     1201 aggctgcctt taccccggcc cgttgcgtag cctgaataac cgcccgacgc tcggtggaag
     1261 taatgccggc aggaaccgaa accaacacct ccgggcgcaa gaattgatac ctcggtatta
     1321 cccggcgcaa aaaataccgt atcatggcca aggtaacccg atagtcggca ataacgccgt
     1381 cgcgcagcgg ccgatacacc tgaatcgtgt cgggcgtgcg ccccgtcatt tcttttgctt
     1441 cttctccaac cgccaaaatg cggttatcca gaatggatac tgccactacg gagggttcgt
     1501 ttaaaaccac cccttttttc gggacaaata ccaatgagtt gcacgttcca agatcgatgc
     1561 caatttttgt aataaacata cgctatgcgc ttttgcgcgt aatattcgcg cctaattgag
     1621 aaagtcgatc ttcaatcttt tcgtatcccc tgtcgacttg gtaaatattg ctaatctcgc
     1681 ttttgccttt ggcgattaag gctgcaataa tcatagcagc tcctccgcgc aaatcaaatg
     1741 tgcccaagtc agtgccaaaa aggcgcgttg gcccattgat gatagccctg tggggatctg
     1801 caatataaat ttcggccccc attttattga gttcttccaa gtaccgcaaa cgccccggat
     1861 acagggggtc gtgcagcagt gtagagcctg ttgcctgcgt tgcaagcact cccagcgggc
     1921 tttgcaaatc cgtatgaatg ccaggataaa taaacgtctg gatcttgtcg atcttgagct
     1981 ttttccaggg caacactttc agatcggcaa gaccatcgtg ttcttgcacc acctctaaag
     2041 gtacgccgaa ctcctttaac ctctttaaga acagttcaag aaaacgggtc tccacatttt
     2101 ttaccactac ctcgcctttt gccgcggctg ccatgataag aaacgtcccg gcttcaatgg
     2161 ggtcaaacat gaggcgatgt ttcattcctg ccaattcccg cttcccgcga acttccatgg
     2221 cgtgcgcccc agacagcgta atgtgagccc ccatttgaga gagcgctttg gtaagttcct
     2281 gaatcgggta atcctggtcg gcggttttta tggtaatagt gccggggtgc aacgccgcaa
     2341 acaacatcat gttttcggtg gcggttacgg aaaattcgtt taaaagtacg gtggtgtccg
     2401 gcaagctttc cggtttttcc agcacgactt ttccattgct ggcaagaatt tttacgccga
     2461 gctgcgaaaa agcatccagg tgcgtgtcta tgggtctggc gccgataata tcgcctcctg
     2521 gtttgggcag actcactttt ccaaatcgcg caagcaaagg ccccagcaaa acaaccgagc
     2581 cgcgaaaacg ggctacaata tctttcttta cttttgtagg atccaaaact tcggtgttta
     2641 ctttcactgc gcgctctctg acccactcaa ttttggcgcc cattccctcg agaatttcca
     2701 gcatgcggta cacatcttcg ataagaggga ggttttcgac aacgcaatcc tgtttggtta
     2761 aaattgttgc agccaaaatc gggaacgctg catttttcgc tcccttagcg tcgatttgcc
     2821 cctttaaagg gcgaccaccc tgtatcacaa atttctccat aagagttgag tttcgggttg
     2881 tattatacct tacttggcgc ggccttgcaa atgatgctat agtactccaa tatgacgaaa
     2941 cgctcaagga cgcttctttt tttggtattt ggggctctct ttgtccttgc agccccggca
     3001 attattttat tcgcacaggg ataccgcttc gactggaaca ccaagcacat tgcgaaagtc
     3061 ggcgcgtttt attttgcggt aacccctggc caggttaatg tgcttgtgaa cggggaattt
     3121 gtcggaacta ccgccccatt gcgcgagaat atcttcattt cgaatctttt gccgcagaca
     3181 tataatgtgg aaatccagaa aaccggtttc caaacctggc agaaaaatct tctccttgcg
     3241 ccaaagcagg ttactgaagc taaacatatt atattattcc ctgaacatac aagctttcgg
     3301 gcgctgcaag aaaatgtcgc agagatgtgg ctggctccca atcagaacca ggcggtgata
     3361 aaaaaaccca tttcacggac tctttgggag ctttccttgt gggatatgga gcgaaacaca
     3421 gtgtaccctc tgtatgtggc gcaaagctcg cgcgatgata ttctcgaaat catttgggct
     3481 gcagactccg cgaagtttct tatgcgcgta gtatctcaag aacaagtaaa aacttttgtg
     3541 cagcatatag acaggcaggg tttgtcgggg cttttgagca ggcaggttgc tctgcagata
     3601 ggcgcccgcg accgggttcc gcttgccccg cttgccaacg agattgtcga tattgcattt
     3661 gcgccttttt ccgaccagca agtcctctac acaaagcctt cggggaacgt ttttgttctt
     3721 tcgctggcag attatttgca agggcaggaa atgcagcaga ttgcctcaag catgataacg
     3781 tttgggctgc agggacaaag tattttttgg ctggacgaca aagggactgt ttggaaaaag
     3841 acaagcctca atgcagaagc tacgcctata aactcttctc ctttgcctat gcgtgcggaa
     3901 acgtcgtacg aattatacgc gtttggtgac gaaatcctga tcaaggaatt tcaaaagctc
     3961 taccgcttag agcgggaaac ccaaacattc aaggaaatgt ttttgagtgc gggaacgatt
     4021 gtcgagaacc ccgaccggca aaaagtggct atcgcaaacg gctctgaaat atggatttta
     4081 ttccttgcag accaagccga acagccaatc caccagaaag gagaaacggt atttcttacc
     4141 cgattcagcg cgccggttga gaatcttggc tggataactt cttggcacct gcttttctct
     4201 gtccaagata ccgttaaaat taccgagatt gatgagcgcg acaggccggt aaccatgaat
     4261 attggccagt ttgcggctcc ccagcttttc tggcagaata ccccagccac catatctgta
     4321 gtgaacaacg gcaccgtttc tgtctctgaa actttgatac cctaaccaaa accccgccct
     4381 gagcgtagtc gagaggcggg gtagacgaga tggaaactat cgtttcctcc attggcgggc
     4441 gcgacgggcg cgctttaagc cgaatttctt tcgctcgcgc atcctagagt ctcttgtaag
     4501 aaaacccaga gttttcaggt ttgtccgcaa atcagcatcg aattttacca aagctcgggc
     4561 aattccctga cgaattgctt ctgcctgcgc atgagggccg cctcccgaga cttttgccgt
     4621 aacgctaaaa gtctttccgg caactccctt ttccaaagct tctctggcgg ttttctggta
     4681 ctcggtagat tgcacatgta aagccagggg ctttgcgttt acggtaaagt cttgctttgc
     4741 gctctcaaat aagcgcaccc ttgcaaccgc ggtttttctt cttccaaccg cttcgtaaaa
     4801 aatgcttggt ttttgctgtt cggtttttgt ttgagcagtc cttggcatag gctagcttat
     4861 tttcaaacgg gttatctgct tggaacgaag cttgttgttt ggcaacatgc cccatacggc
     4921 tttccttaac acttgggcag ggcgttcggc aaacagcgtc cccaactttt tttcttttaa
     4981 cccccccaaa tacccggaat gtctgtagta gattttctgg ctcatcttgt ttccgctgaa
     5041 tgcaagctct cgcacgtttg tcaccacaac aaaatcaccc atatctttgt aaggttcaaa
     5101 atcgggtttg tgcttgccgc gcagcaatat cgctatctgg gtggcaacgc ggccaagcct
     5161 ttggtttttt gcgtctatgg tgtgagtggt gcggttcatt tgatgagttc aatgattgcc
     5221 atttttgcgc cgtctgattt tcggaacatt gttttggtaa tacgggtata tccacccggt
     5281 cggtctttgt agcggggccc gatttcttct accatttttt tggtaaggcg ggggccgaaa
     5341 taacgaagca actcccttcg ggtagccaaa gtgtctcgct ttgattttgt taccagcctt
     5401 tcaaacaaag gggatgtttc ttttgccttt gcctctgttg tttcaattct ttctttcaaa
     5461 gccaacgcgc gcaccaaaga cagcatcaat ccctctctgg cgcctttcga tcttgaaaat
     5521 attcgacctg cttttcgctt cttcatggaa agttatttct ttacggtctt tttcacggat
     5581 ttctttgcgg cttttttcgg cttttctgca tcttctttag gctcttcttt ttgccgcacg
     5641 gttttttgcg cttccagcgc tcccttcacg ccgtcttgaa ctatttggaa ttctttcagc
     5701 aaaatatcgg tggcgtcgaa aaacgcctgt tcggggcttt gagtgccgtc ggtttccacc
     5761 tcaatggtta ctcgattaaa gtcggtgcgg tctcccacgc gcatgttctc caccctggaa
     5821 cttacccgtt taataggggt aaaaatagcg tcgagcacaa tggttccaat atcttgtttt
     5881 tccttgtttt gctcctctgc gggtacatac cctacgcctt tctcaacctg aatttccaat
     5941 tcgagttccg cagatttttc ggtaagatgg gcgagaacca aatcctggtt gagcagctcc
     6001 aattgggtgg gaagctcaaa gtcagctccg gttatctctt tttcgccttt cactttgagc
     6061 gtggcttttt gaggctcttc tgcgtgcatg tgaaaccgaa gttgcttaag ctgcaacaaa
     6121 accgacacga tgtcttccat aagcccggga agagtggaaa attcgtggtc cacgcctttt
     6181 atcttcactt tcgttactgc agctccttcc aacgaagaaa ggagtacgcg ccgtaagctg
     6241 ttgcccacgg taactccata ccctggatat aaatcctcaa taacaaaaat ccctctgttg
     6301 tttttgcttt cgacgatttt ggtagattga gataaaggaa tcatatggtt atcttgagta
     6361 aaactcaaat acgaggggga gctggaccgt aggagcaatt tcctcgagtc ctggctcacc
     6421 cattatttcg cccttcatgt tggccttgtc caattggatc cacgaaggag actgatattt
     6481 ttttaatgcc aattttacgt tttgaaacaa cgtaccggcc gcagattgcg gtcttatttc
     6541 aataacgtcg ccgcgcttta ccctgtagga aggaatatcc accaccctgc catttacaaa
     6601 aaaatggccg tgggcaacca attgccttcc ttgtttgcgc gtttgggcca ttcccatgcg
     6661 gaacaccaca ttatccaaac gcttctctaa agcttgcata aagagttctt cggcattccc
     6721 tgtggaacct ttggccaggg tatcggcaat gtattggcta aactggcgct cgcgcaaatt
     6781 gtactgctct ttgagctcct gtttttcccg caactgcctt ccgtattcag aaaggggcga
     6841 agtccttctt tttcttacaa cacctttttg cataaatata tcgtttacac ccttctcacc
     6901 ttagccgcgc gcggcccatt atgaggcaca ggcgtagtgt cttcaattga taaaatcgca
     6961 aacccttttg cgccaagcgc gcgcaaagcc gaatctcgcc cggcgccaac ccctttgaga
     7021 aacacttcga gttcctccac gcctttgttt ttggcaattt gcgtgagagc gtcggccacc
     7081 ttggaagctg caaacggggt ggatttcttg gtccctttaa agcctatagc tcccgcggaa
     7141 acctgggcca acacgcgccc ggtgcggtcg gttaaggtca ttatgatatt gttatacgaa
     7201 gaatacacat atacacgccc ctctcgtact ttctttgagg agctctgggg gccaatttgc
     7261 ttggcctctg gtgctggcgc ttcttcgccc tttttgataa ttcgtttttt tcccatagga
     7321 ctatgttgga cttgcagcgg gtttcctgcc agacgttacg gtttttctta cattgcctcg
     7381 cacggttcgg ttgttcgttc gcgttctctg gccgcgcacg ggtaaatgct tggcgtggcg
     7441 aactccccgc caagcgccaa tcgcgtgaag gcgtcgaata ttcatcatga tatcacggcg
     7501 taattctccc tcgatacggt agtttttttc gattacttct tttagcgcgt tgagttcttc
     7561 ggtctttaat tgcgaagcgc gttttatcgg gtcgattctc gctgcagcaa gaattttctt
     7621 gctcaaagca ggccctattc cgtaaatata ggttaacgca attcctattt gtttattgtc
     7681 cgggatgttt atccctacca gtcgtggcat agcgaattta cccctgtctt tgcttatgct
     7741 tggggttttt cttgcatgtt acatatatta ttcctgcgcg ttttgcaatt ttgcaatctt
     7801 tgcagatttt tttcactgaa gattgtactc tcatggaatt actttccccg gtagacgatt
     7861 ctcccgcgcc tggtatcgta aggggtcatt tcaatcgtta ccttgtcgcc cggcagaatg
     7921 cggattttat gcatccggag cttgcctgaa acataggcga gaacttctgc gccatcatca
     7981 agcttaaccc gaaattgcaa actcggcaag gcctccaaaa cctctcctgt tttcctcatt
     8041 tcgctttgat tgccgtcaat catcaagata gtctatatgg tagcacagtt aaaacgccag
     8101 gtcaaggtgt atgcgctctg cgctctgggg cacatgctcc acccaaaatg gccagaaaga
     8161 aaactcggct ttggcaagat acgggtattg cctgaatact tccgtaaacc cgtttttctt
     8221 tacgccctgg agcttgcctt tcaaatcctg catgtccagg gcgtcatact ggtccacatg
     8281 aatcataacc tctatggata aagcgccggc gtttgcagtg ggctggtcaa ccttatacgc
     8341 tatttcgagg gtctgttcat tcaccgattg cccctcgtcc agactgtctt gcactatgcg
     8401 ttttgcaagc aaatccgcct catttttttg aaagcccaag gcggaaactt tcacctttgc
     8461 tgaatagtta aactgggtga gctcggcgcc ctcatttacc aaagcgccgt cttggagaac
     8521 tacagaagtt acagaatctg caaccacctg cacgttcccc ggaatccgcc ccagcaggct
     8581 ttgcgttgcc tgcactttga gagcctcgat aagttgctct ctcgcagatt ctacgtcttc
     8641 tcgggtaacc acggccacat gggtttgggt gccgcccttc atgggctgaa acgactcacc
     8701 gtaaattgcc gtgtacagtg cagtacccgc gagcccggga agggaaaact tagaggcctc
     8761 gacattatat tcctggcccg attctgcggc taccacatcg acatcaacat acccggagcg
     8821 cggaactatg gcttgccttt ctgaacgaaa cagcctgcct tcttccgata taaaccgggt
     8881 gctcgccacc agtgtttgag acgcgccaga gctcgtatta tacacgcgaa tagaaccgtg
     8941 cgcccggtca tctttttcct gggtgcccgt agcaggaaat acccgggtca tttctttttc
     9001 ttcttcgaag gtttgaacct caacagtaat ccgctcgttg atttcaagcc tggttatctg
     9061 gggccatact ttaatatcgg cgcgcgcgaa catgaaatgg agcaagataa ccggaacaac
     9121 cgagagcaaa attgccaaaa cgaaaaacct cacccaaacc gttggtgcct tgctcgccaa
     9181 agaaggtttg gcttgttttt tttcgaacag ttccgcgtca gccagctttt cttctctgct
     9241 ttggggaggg aaaatatcga taagtttttt cttcatatca tgctaccaat acggaatcgg
     9301 caactaaaca taacgaggaa tattggggat tgtgattttg tgcaaaagaa acaccttccc
     9361 atatatcctt gggtgccaaa atatgcacat tgatatcttc cgtgaatggc aggccttttg
     9421 ccagttgcgc ctgcaaaact tcctggaaat ggtgagtttt tattcccctg ccaaaaatcc
     9481 agatgtttcc gggaaggggc gttttcatca cttcaagctg tttctttatt agtgtaacaa
     9541 acttttggaa ggtgggcaat aaaagagaat cgattttttc ctttacttgc gtggaaagag
     9601 cgccctcgac gtagcgtttt tcaaattcga acgcgatatt gtcgcgcata ccaagcactt
     9661 cgccaaacac ctccacaaaa tcttcctcgc ccagggctaa agaagtttcg ggaagtaaga
     9721 gcacgccctg cttccacacc accagctgtg aatgccgcct cccgacatac agatatactc
     9781 cctcgcgact cgtttttcgg ccaaagtcgg ctattgcctc ggccatatgc cgtactcgaa
     9841 ccgtatgcag ttttttcgac tcaacaaatt cggcaagatc aaaaaaagtg gtagctaaca
     9901 aaaaagtggc taggaaagag gcgcgaattt cccccgcttt gaaaccctct aaccgaggca
     9961 cggaatagcc gtcaattttc tggctggtgg ggactaatcg cagcatccga aaatctgcag
    10021 gaagaatgcc cgattcccca aacaagttac cgaaaagttt tgcctttgcc tgctggcgca
    10081 tggttttttc aatccagagc gcctcttttt tgtcgagcac atgccgcgct cccctgcccg
    10141 gaaaaactat ctcaattgac tttgctttaa agtgatcggg tgaaaaggta gcaataaggg
    10201 tgcccacgga aacttcccgg ggaagttgcg caataacctg gcccaggctt tcttgcaagc
    10261 cacccgcggt tacctgttgg tttccatatt tgagtacctg ctttttatcg gccatttgcc
    10321 taaaaaccaa aaaattgagc gtgtcgccgt cgagcgcaac tgccaaaacg ctccctcctg
    10381 atttttgccg ttgaggagac caaaaaccca tattctttac attatacaat acatttgtaa
    10441 aggtgaaaac aaaatgctct gcaaaaataa aaccccgacc gttttggctt tccgttcggc
    10501 cggtagaaat ggttgagtaa gtgagtaaag ttaaacctta ctcaagaatg gcgcggaggc
    10561 ggcggatgcc ggaggaaacg gattcttctt tggtaatttt aaagtggccg agttctgcgg
    10621 tgtgttcgac atgcgggcca ccgcaaaatt ctttgctaaa ggcgtttccc tgtatgtcct
    10681 gggcaacata aaccgaaaca acatcgccat actttacgcc aaactccatt tcggcgccca
    10741 tttgcatggc ttcttcttta ggcatttctt tacgaatcat atccaggttt tctgcgattt
    10801 tttggttcac caaatcctct atttgtttta actcctctgg cgtaactttt gcaggatgcg
    10861 aaaagtctat acgcaagcgt tctgcggtaa tgttgctgcc ccgttggtgc acatggtcgc
    10921 ccaaaacctg ccttaaggca gccagcagca agtggtgtgc cgtgtgcagg cgtaccgtct
    10981 cttctttggt atctgccaat ccccctttaa acttcttttc agccccggcc cgtgaaattt
    11041 ctcggtgctt ttttagctct tctctaaaac cgttttcatc caccgtagtc cccttttctc
    11101 gcgctaaatc caacgtaacc tccaggggaa aaccataact ttggtacaaa ttaaacgctt
    11161 ctcgcccgga aatattatta tcgcttagtt tttcaaattc ttttaggccg tttgctaatg
    11221 tttctcgaaa ccggttttct tccttttgga tagtttcgat aattagcgta cgatgtagtt
    11281 ctaattctgg atactgattc ttatagttct caataacggg gcctgaaagc tcggcaaact
    11341 tgccttgttc cattcccaaa acatccgcat accgcacggc acggcgcaaa agcctccgca
    11401 caaaatagcc tcggtcggta ttcgaaggca aaacgccgtc gccaatcata aacacggcac
    11461 cgcggatatg gtctgcgata acacggaaag cgtacttggg atcgccctca tatttctttc
    11521 cagacaattg ttctattcgc gcaataatgc tttgaagcgt gtctatgcta aaaatatcgc
    11581 tattgtcgtt tgaggcagct gcaatgcgct ctaatccccc gccgaaatcc acgttgcgct
    11641 gggataattt ggcaaaagtt tcatcctgct gcttttgata ttccatgaat accgaatttc
    11701 ctatctccaa aaacctgcca caatcgcagt ttggatggca ctgttcccca aatttcgtat
    11761 cgtgctgcac gtcggtgaat tcaaaaaata cttcagaatc cgggccgcca ggctcgcctg
    11821 caggcatatt ttccggcacg ccagccctgc tccaccaatt tttcttggcg ccgtaaaaca
    11881 aaatgtggtc ttttggaata cccagttttt tccatatggc tgccgattct tcgtccttgg
    11941 gaatattgtt tttctcgtcg ccctcaaaac atgttaccca tagcctgtct tttggcaagc
    12001 cgactttttg ggtaagaaat tcaaaaaacc agtgaagttg ttcttcctta aaatagtcgc
    12061 ccagcgacca gtttcccagc atttcaaaaa atgtggtgtg gcggttgtcg cccacttcgt
    12121 cgatatcctc ggcgcgaaag cacttttggg agtctgctaa acgggttcct gccgggtgct
    12181 tttgccccag caaataagga atcaagggtt gcatgccaga tccggtaaaa agcgtagtag
    12241 ggtcgttttc gggcaccaaa gccgccgagg gaattacttt atgctccttc tcctcaaaaa
    12301 atttcaaata tgcatcccga atttcgtttg aagtcatatt ttgctagtat accaaaaacg
    12361 ctactcgatt acaccgcccg cgatcatttg atctccctgg tacagcacag ccatttggcc
    12421 agaggtaatc gcgcgttggg gactgtcgaa tatcaacttt cctgccgtaa cagacttgcc
    12481 tattcgaaaa gtgcccgcgg ctggtttttg acgataacgg atacgggcat gcaccaagtc
    12541 ttgttttggc tcaacaaacc agttaaggtt cttcagtctt acttcctttt gaagcagatg
    12601 gtcttcattt ttactgacgg taacggtatt tttctttata tctttatcca ccacaaaata
    12661 gggcccgcca gccaaaccta agccttttcg ttggccaatt gtataataca cagctccctc
    12721 atgctgccca agtatgttgc cttgggtatc tacaatattt ccctgcctga caggaatgta
    12781 ttctttcaaa aaatctacga aatctacttt gcccacaaag caaatgccct ggctatcttt
    12841 gcgctcggca ttgggcaagc caaacttctt ggcgagtttg cgaacttcgg gcttggtata
    12901 gtcgccaatg ggaaacatta ccttatctag aacctcgggt tttataaacg acaaaaaata
    12961 agattggtct ttgttttgat cttttgccat aaagatagat ccc
//
LOCUS       DASRUP010000102        11656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2269916, whole genome shotgun sequence.
ACCESSION   DASRUP010000102 DASRUP010000000
VERSION     DASRUP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269916"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2103..2678
                     /locus_tag="VFE94_04015"
     CDS             2103..2678
                     /locus_tag="VFE94_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50275.1"
                     /translation="MARPLFGGWMKWVNISGIAVILAILLAYAALQLAPLLKSSSQSL
                     EPAPATDVTEPANTYAETYAPDTILDPAVVEPATWPSYPAELVGTWAIVTAKGERLDS
                     LMVLSAEGEFGGTDRYAGTWYSPQEGTIHATISGADTKYTYTLAHEGGETFLMLWDAQ
                     PTQNPRLQPWARNHVKIADEPKFLAAPSQGQ"
     gene            complement(2751..5234)
                     /gene="gyrA"
                     /locus_tag="VFE94_04020"
     CDS             complement(2751..5234)
                     /gene="gyrA"
                     /locus_tag="VFE94_04020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZX50276.1"
                     /translation="MADDTKKPGLGQVETREIVEEMRGSYIDYAMSVIVARALPDVRD
                     GLKPVHRRILFAMQEEGLTHGAKFRKSATVVGSTLGRYHPHGDISVYDALVRMAQDFS
                     MRYPLIDGQGNFGSIDGDPPAAMRYSEARMSRMGELMLQDIGKDTVDFMDNYDGTRKE
                     PTVLPSPLPQLLLNGTLGIAVGMATNVPPHNVRELIDATVYLLDNPKATTEDLFQFVK
                     GPDFPTGGTIYNKREILEAYSQGKGPITIRGKADILEGSEKKRPQIVITEIPYQVNKS
                     SLVEQFAKLVQDKKIDGIKDIRDESDKDGMRVVFDLQRDAQPQKILNRLYKFSDLQRA
                     FHLNMIALIDGLQPKVLSLAEMLQYFVAHRRQVIYRRTKYELDRAKEREHILEGLQKC
                     LANLDEVIRIIRKAADRAEAQLNLMKRFRLTEIQANAILDTRLAQLARLERQKIEDEL
                     KEIRERIKDLESILASPKKIDTVIKKELAELAETFGDDRRTKVVAQAIGEIAEEDLIP
                     QEETIITLTQGGYIKRINPATYKIQKRGGKGILGMKTVSDDIVEHFISAQTHDSLMLF
                     SDSGKVFQTPVYEIPEGTRVAKGRGLLNFLEMSAEDKVLSLFAIGKEDVEAGVKYLVM
                     VTKHGVIKKTPLEDFKNVRRSGLIAISLKKGDALWAVRKTTGQDEIIITTKKGQAIRC
                     KEKDVRPMGRTASGIHGIRLKKGDEVVGMDVIAKNPKLQESKPKQERYVLVVTENGYG
                     KRTDIKEYRLQSRGGSGIKTANITSKTGDLVYACVLSGEEEDLIVISRKGTIIRTAIE
                     SIAKISRSTQGVRIMKLEDGDHVASAACV"
     gene            complement(5244..5897)
                     /locus_tag="VFE94_04025"
     CDS             complement(5244..5897)
                     /locus_tag="VFE94_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX50277.1"
                     /translation="MQIVWKGQACFFLAASRGKQEQVRIVIDPYEESIGLKPPQVEAD
                     MVLVTHDHFDHNNAKAVRGEPMVISGPGEYELKGVFIKGIASWHDNAEGKDRGKNTMY
                     VIEAEGLRLCHLGDIGQDELSAEQVGQIGNVDILFIPVGGTYTVAAKGAAKIISQIEP
                     RMVIPMHYALPRLKVKLEPVDEFLKTMGVKNVEPQAKLSLKAKDFSVEEMKVVVLTP"
     gene            5955..7103
                     /locus_tag="VFE94_04030"
     CDS             5955..7103
                     /locus_tag="VFE94_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZX50278.1"
                     /translation="MTKTAVLDKPKLEIIKPFRVGDLVEGTVVGIGRSALYLDLGPQG
                     TGIIYGREFLEERGTLKDLKVDEKLTAKIVDLENDKGYIELSLKEAGRELTWDRLKGK
                     KDSEEIFKVKIIGANKGGLLAELYNVQAFLPVSQLSQEHYPRVEDGDATKILRALQAF
                     MGTELEVQIFDLDPREEKIILSERSKERAKVKEQLARFKVGDVVEGEITGVVDFGAFI
                     KFPLGDPATEPVEGLIHISEIDWQIIEDPNQLLKVGDKVKAKIVDLSQGRASLSLKAL
                     KEDPWKEVEKKYKKFDKVEGKVTKLNPFGAFVEIEEKIQGLCHISEFGTKSKMEEMLK
                     AGQSYPFQILELNPSEHRMSLRLVVPGAETDQATESTEPMPEQQPVQE"
     gene            complement(6991..7752)
                     /locus_tag="VFE94_04035"
     CDS             complement(6991..7752)
                     /locus_tag="VFE94_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HZX50279.1"
                     /translation="MKIGLVLFLVLVPSPGLAQVVINEVAWMGVPVESVEERQWWRYE
                     WLELYNAGAEPVSLNGWVLELSRDKVDLHVSLRGSIEPQGYFVVAASSKIPGADVNYA
                     SLSGKFVNSGQKVQLKNAAGELVEEVDAKAGWFAGDNDVKSTMERRFPERESNNKENW
                     GSSQTLGGTPKVQNSVFGKEKVLVLQANASAGSTDLPQAKKAFPGASFVETATNKVFI
                     LALAVALASALSILLLGRFLLREQPGEGSSGALKD"
     gene            complement(7839..9266)
                     /gene="truD"
                     /locus_tag="VFE94_04040"
     CDS             complement(7839..9266)
                     /gene="truD"
                     /locus_tag="VFE94_04040"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HZX50280.1"
                     /translation="MRREEKEQEHQLLLAEKKRVPELFEMPPMIDTLQLLQWIGIEGV
                     FGEYPFGHLKYLPEDFIVEEISLDKKISTVDIGSGVQNLQEQEGSTIYAELVKVGLTT
                     FHAKDEIARFVEIPSQSIGTAGLKDEVAITSQRISLRKVRNLQLLENLAFEDFFVKNI
                     VRGKGVLKKGQLWGNRFVITIRFAGPLQAAEKLRIEQSLKEISEQGFWNFYSFQRFAA
                     PRFNGHIMGRHLLRGEYEQAIRTLLFYRGGREPMYFQHIRKDTENHWGNWKYIYQQMR
                     SFPSQFPTELQFLEYLVERPNDLRGALRAIAEQVRLWLYAYSSYLFNKKLSQSIKEGE
                     ILFELPFVTSGNPKDWEHYEKFLKQDGITLPTSLYRDLPFLRSYSAGIFPTAPTLQQV
                     EIHKAVFLENMMVLAFSLPKGAYATTFLAHLFQLSSEVPLPPGVSTNRIDAKEVVGLG
                     SLGPVLERFRPVITRFEQAREPKTN"
     gene            complement(9374..9961)
                     /locus_tag="VFE94_04045"
     CDS             complement(9374..9961)
                     /locus_tag="VFE94_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50281.1"
                     /translation="MLRFTINPNQSKRLEQAGIRRIVLDREALEKEHVATAKRVGRDP
                     YLYDTDRRHVADAGVRVFGEKDPEKLCVLPAWEEMEKSGYQLVDVQLTERRAGGMDIL
                     TLVVSNMLDMPLIELSDLAWERVNMCLGCRWRYTTVWANPRGRDTLIRELAVDDWTNR
                     FGEDGYIVHTVNTRHRSDERPSHTLGFAGGLWELD"
     gene            10280..>11656
                     /gene="ftsH"
                     /locus_tag="VFE94_04050"
     CDS             10280..>11656
                     /gene="ftsH"
                     /locus_tag="VFE94_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZX50282.1"
                     /translation="MKKLSKNFLFVVLILLSISALFALLSDPFGATEQVPLSQVVQDI
                     NADQIEKIVVSGNKLVITYKDDSTKESTKEAQGALPETLIDLGVQQDKLNTVTIEPRE
                     ESGFVTLLGPLTFILLPVLLFFVFFWMMFRQARSGIGQAFDFTKARAKLFGAEGQLKQ
                     KVSFKDVAGLKEAKEEVQEVVDFLKFPKKYLQMGAKIPRGVLLVGPPGTGKTLLAKAV
                     ASEAGVSFFSASGSEFIEMFVGVGSSRVRDLFQTAKKSGRSIIFIDELDAIGRTRGFG
                     FGGGHDEREQTLNQILVEMDGFERDDTQIVMGATNRPDVLDPALLRPGRFDRTIILDL
                     PDVNDREQILQIHAEGKPLAPNVRLREIAERTPGFSGADLANLMNEAAILTARRNKQQ
                     VSQAELFESIDKVLLGPERKSHVLSKKEKEVTAYHEAGHALVAASLRHVDPVRKISII
                     SRGRAGGYT"
BASE COUNT         3051 a         2976 c         2657 g         2972 t
ORIGIN      
        1 cccaccggcc cgcccggccc cgacggatcg cagtgtccag tggttgtatt gcatgtaaat
       61 ccaacgttac acgaacccgg cgtacaagcc ggcacgcata gtcccggcgg attttgacat
      121 accggtcctg ctggatcacc aacacagtcc gaatcgtctg aacaaactcc tgatcctggt
      181 gcacacgccc cgccagtagg tggaagactt gccgtgcacg atggctcaga tgccgtacga
      241 catgacggta tcttcgattc gcttaaaatg gtcacctctg catcgtcggt attggcacca
      301 ctgcatgtaa tccctacctt aacccaataa ttcgaaccaa aagcaacgtt atttagatcg
      361 ctgactccac tgagtctaaa tgtaatcgta ccggggggag taccgcttgg taattttgaa
      421 cctacattca cataccctgt ccctgttttt gagctgcagg ttgtagcatt tgtttgagta
      481 aaggtaaaat caagatcgcc cccattatgt acgtaggtgt ccgccgaagt acacgttgga
      541 gttccctgca acccactccc agcaaccgat ccagcagtta cggttaccgc gccccctggc
      601 ggggcagaac tcacaactac ttcagctgaa gctggcaatg ccccgcttcc atcgcttaag
      661 tcaaacgtat acctaccagg ctgtgttaga ggaaaagtac tactgccagc aatattccca
      721 ctggcatcac tgcagctatc gagaggtggg cctggccccc acatatcatt aacagcatta
      781 acgaaagatc catctcgaaa agtttctttt ctacaggggc caggagctcc tgcaggaaga
      841 ctccatgata ccgtaacatc atctccaggc gctatttgcc cgcggcttac ttgaacactc
      901 aagccacctc cggggccacc agaaataact acagtaagtg tcgctgtatc ggtttgttga
      961 ggactgctgg tatcggtaag agttaaggta acggtataag accctggccc gggaaacgtg
     1021 tgcggccctg gatctatacc agtgtcggta cctccgggat ggccatagtc ccaagtggcg
     1081 ctatatggct tggtacctcc gaacgatgac gctccagtaa aatcccgaac caagccagat
     1141 gctgattgaa aaccgataac cgcgtttagg gcagacgctc ctccagaagt ggtaaagctc
     1201 tgaaccgagc caacaaccgt attagcagaa ttgcgcgcaa aggcgcagaa tcgatagtcc
     1261 cttccagcag taagccctgt aatggctcga ctgaaaggtt ggtcagtaac aggaccaaaa
     1321 ctcgacgttg gtgttgttat acctgacatc cctacagaac agttagaagg aaatgataaa
     1381 gcgggagcat aaacaaacca aacatcaaca gagatagacg aagtaacctc tcccctaaga
     1441 gtcgcgccag tcgatgtaat gcttgttgct gggtcggtaa agacttgggg aggagaagcg
     1501 ccactagtga cggtcactaa ggtactggca gatcgcgtaa tgccctgcct tgtaacagta
     1561 accaaaggag aataagtccc tggcgcgtta tatgtacact tcgtagcaat agttttggga
     1621 gaaaccgtgt caacgccacc ggtattaaaa caactatccg tgggcccatc tctccaatct
     1681 atcgaccagt tgtaacttcc ggtcgcagtc cccgtaagag taaaggatac ggttatgggt
     1741 ccaagtgggg cagcacccga attagggttg gcactaggcg tgacgaaaag ggtttgcgca
     1801 gcgacagacg atggaagaaa aacaaaaaac aggacggcgg taaagacgac ggcgagagag
     1861 gcaaaaaaaa gtttcttttg cattagcata ttatacccca atacaaactc tttgggaata
     1921 caaaatagtt ttccacaagc gcgaaatgtt ttgcaaactt tagtttttaa agcaaatatg
     1981 cgtttttgct ttgtaaaact tgacagttcc gataaaaagc aggtataatt ataggtatag
     2041 accttaccat ttgtatactc aatactcact aaggaggtga gaacgtggct agaatagcta
     2101 ctatggcacg acctctgttt ggtgggtgga tgaagtgggt aaatattagc ggcatcgcag
     2161 tcatcctggc tatacttctg gcgtacgctg ccttgcagct tgcacctctg cttaagagct
     2221 caagtcaaag cttggaaccg gctccggcaa cagacgttac cgagccagcg aacacgtatg
     2281 cggaaaccta tgctcctgat acgattttgg atcccgcggt cgtggagccg gcaacctggc
     2341 ccagctatcc tgcagaactc gtaggcactt gggcgatagt aaccgccaaa ggtgaacgtc
     2401 tcgacagcct catggttctc tcggcagaag gcgaatttgg aggcaccgat agatatgcag
     2461 ggacttggta ttccccacag gaagggacaa tacacgccac tattagcggc gcggatacta
     2521 agtacacata taccctagct catgaaggtg gagagacctt cctaatgctc tgggatgccc
     2581 agccgactca aaaccccagg ctccaaccct gggccagaaa ccacgtaaag attgcagacg
     2641 agcctaaatt tctcgctgcc ccttcgcaag ggcagtagca cactagcata aacactgcgg
     2701 caaggccgca cccatagagg acgaacccat tcgccctctt tctatttgcc ttatacgcag
     2761 gcggcagagg ccacgtggtc gccgtcttcc aacttcataa tgcgcactcc ttgcgtggaa
     2821 cggctgattt ttgcgataga ctcaatggca gtgcgaatta tggtgccttt gcgggatatg
     2881 acaataaggt cttcctcttc gcccgaaagc acgcaggcat ataccaagtc tccggttttt
     2941 gaagttatat ttgccgtttt aattccagaa cctccgcggc tttgcaaacg atattcttta
     3001 atatccgtgc gcttgccgta tccgttttct gtcaccacca atacataccg ctcttgctta
     3061 ggttttgatt cctggagttt tggatttttt gcaataacat ccattcccac aacttcgtcg
     3121 cctttcttta agcgaatgcc gtgaatgcct gaagccgtgc gccccattgg ccgaacatcc
     3181 ttttctttac accggattgc ctggcccttt ttcgtggtaa taataatttc gtcttggccc
     3241 gtggttttgc gcacagccca caaagcgtct cccttcttta aagaaatggc aattaagcca
     3301 gaccgccgca cgtttttaaa atcttccaac ggagtctttt ttatcactcc gtgtttggta
     3361 accatcacca gatattttac tccggcctcc acgtcttctt tgccaatggc aaacagcgac
     3421 agcactttgt cttctgcaga catttcaaga aaattcagca agcctcttcc ctttgccacg
     3481 cgcgtgcctt ccgggatttc atacacgggc gtctggaaca ctttgccgga atctgaaaac
     3541 agcattaaag agtcgtgcgt ctgggcggaa ataaagtgct ccacaatgtc gtcggacacg
     3601 gttttcattc ccaaaatgcc tttgcctccg cgcttttgga ttttgtaagt ggcagggtta
     3661 atacgcttaa tatacccgcc ttgggtaagc gtgataatgg tttcttcctg cggaatcaag
     3721 tcttcttctg caatttcgcc aatggcttgc gccactactt ttgtgcgccg gtcgtcgcca
     3781 aaggtttcgg caagttctgc aagttctttt ttaataaccg tatcgatttt cttggggctt
     3841 gccaaaatac tctccaaatc tttaattctc tcccggattt cttttaattc atcttcaatt
     3901 ttctggcgtt ccaagcgggc caactgtgca agtcttgtgt ctaaaatggc gttcgcctga
     3961 atttctgtta ggcgaaaacg cttcatcaag tttagttgcg cctcggctcg gtctgccgct
     4021 tttctgataa tgcgtatcac ttcgtcgaga tttgccaaac atttctgcaa accctccaaa
     4081 atatgctctc gttctttggc gcggtccagt tcgtacttgg ttctgcggta tattacctgt
     4141 cttcggtgcg ccacaaaata ctgcagcatt tcggccaacg aaagcacctt gggctgcagg
     4201 ccatctatca aagcaatcat gtttaagtga aaggcgcgct gaagatcgga aaacttatac
     4261 aaacgattca aaatcttttg gggctgggca tcacgctgca agtcaaacac gacgcgcatg
     4321 ccatctttgt ccgactcgtc tctgatatct ttaatgccgt cgattttctt gtcttgcacc
     4381 aactttgcaa actgctccac caagctggac ttgtttacct gatagggaat ttcggtaatc
     4441 acaatttgcg ggcgcttctt ttcagaacct tccagaatat ctgcctttcc ccgtatggta
     4501 atggggcctt tgccttggga gtaagcctca agaatctccc gtttattata aatagtgccg
     4561 cccgtaggaa aatcgggccc tttcacaaac tggaataaat cttcggtggt ggctttggga
     4621 ttgtctaaaa gatataccgt ggcatcaata agttctcgca cgttatgcgg aggcacattg
     4681 gttgccatac ccacagcaat accaagagtg ccgttcagca agagttgagg caagggcgag
     4741 ggcaacaccg taggctcctt tctcgtgccg tcgtagttgt ccatgaaatc caccgtgtct
     4801 ttgccaatgt cctgcagcat taactcgccc atgcgcgaca tgcgggcttc cgagtaccgc
     4861 atggccgccg gcgggtcgcc atcaatagaa ccaaagttgc cctggccgtc gattaagggg
     4921 tatctcatgg aaaaatcctg cgccatgcgt actaaagcat catacacgga aatatctcca
     4981 tgaggatggt aacgtcccaa tgtggatccc acaactgttg cagacttgcg gaatttggcg
     5041 ccgtgcgtta agccttcttc ctgcatggca aacaaaatac gcctatgtac aggctttaag
     5101 ccgtccctta cgtcgggcaa cgcgcgcgcc acaatcaccg acattgcgta gtcgatgtac
     5161 gaccctcgca tctcctcgac aatctcgcga gtctccacct ggcccaatcc tggttttttg
     5221 gtgtcgtctg ccattgtttt tccctaggga gtaagcacca ctaccttcat ttcttctacc
     5281 gaaaaatcct ttgctttcaa agacagtttt gcttgaggct ctacgttttt tacccccatt
     5341 gtttttaaaa actcgtcgac aggctctagc tttactttaa gcctgggcaa cgcatagtgc
     5401 ataggaatta ccattcgggg ctcgatttgg cttataattt tggcagcccc cttggctgct
     5461 acggtatatg tcccacctac cggaataaac aagatgtcca catttcctat ctgccccacc
     5521 tgctctgccg aaagctcatc ttgccctata tcgcccaggt ggcaaagcct caacccttcg
     5581 gcctctatta catacatcgt attctttccg cggtctttgc cctctgcgtt gtcgtgccag
     5641 gaagcaatac ctttaataaa cacgcctttg agttcgtatt caccgggacc cgaaataacc
     5701 atgggttccc ctcttactgc ttttgcattg ttgtggtcga aatggtcatg agttaccaac
     5761 accatatctg cctccacctg gggaggtttt aacccaatag attcttcgta agggtcgata
     5821 acaattcgta cctgttcctg ctttccccga gaagccgcaa ggaaaaagca tgcttgtccc
     5881 ttccaaacta tctgcatgat tttgcattgt accacctctt gcaaaaaaac tcaatctctt
     5941 gtaaggtata gggtatgaca aaaaccgcgg ttctggacaa accgaagctg gaaattatta
     6001 aacccttcag agtgggcgac ctggtagagg gcaccgtagt tggcattggc cgttcggctt
     6061 tgtatttgga tctgggcccg cagggcaccg gcattatcta tggccgcgaa tttctcgaag
     6121 aaaggggaac tctcaaagac cttaaggtag acgagaaact gaccgcaaaa atcgtagact
     6181 tggagaacga caagggctat attgagcttt cgctcaaaga agcgggaaga gaattaacgt
     6241 gggaccgcct caagggaaaa aaggactccg aagaaatttt caaggtaaag attattggag
     6301 cgaacaaggg cggccttttg gcagagcttt acaacgtgca ggcgtttttg cccgtttccc
     6361 agctttccca ggaacactac ccccgcgtgg aagatggcga cgccaccaaa atcttgcgcg
     6421 cgctgcaggc ctttatgggc acagagctgg aagtacagat ttttgacttg gaccccagag
     6481 aagaaaaaat cattctttct gaacgatcca aagaacgcgc aaaagtaaaa gagcagcttg
     6541 cgcgctttaa ggtgggcgac gtggtggaag gagaaattac cggcgtggtg gattttggag
     6601 cgtttatcaa atttccgctc ggagaccccg caacagaacc ggtagaaggc cttatccata
     6661 tttcagaaat cgactggcaa attatcgaag atcccaacca gcttttgaaa gtgggcgaca
     6721 aagtgaaagc aaagattgtg gacttgtcac aaggcagggc ttcgctttcc ttaaaagcgc
     6781 ttaaagaaga cccctggaaa gaagtagaaa agaaatacaa gaaattcgac aaagtagaag
     6841 ggaaagttac caagcttaat ccttttggcg cctttgtaga aatcgaagaa aaaattcagg
     6901 gcctgtgcca catttcagaa ttcggcacca agtcaaaaat ggaagaaatg ttaaaggcag
     6961 ggcaatctta cccattccaa attttggagc tcaatccttc agagcaccgg atgagccttc
     7021 gcctggttgt tcccggagca gaaaccgacc aagcaacaga atcgacagag ccgatgccag
     7081 agcaacagcc agtgcaagaa taaacacttt attcgttgca gtttccacaa acgaggcccc
     7141 aggaaaggcc ttttttgctt gtggcaaatc tgtggatcct gccgaagcat ttgcctgcag
     7201 caccaaaact ttttctttgc caaacacact gttttggact ttgggggttc cgcctaaggt
     7261 ttgggaggaa ccccagtttt ctttgttgtt agattcgcgc tcgggaaatc ttcgttccat
     7321 agtggacttc acatcgttgt cgcctgcaaa ccacccggcc ttggcatcaa cttcttccac
     7381 cagctcgccc gcagcatttt tgagctgtac tttttgcccc gaatttacaa attttcctga
     7441 caagctcgca taattcacat cggccccggg gattttacta gaagcggcca ctacaaaata
     7501 tccttgaggc tcaatgctgc cccgcaaaga aacatgcaaa tccaccttgt cccgcgataa
     7561 ttccaggacc cacccattca aagaaaccgg ctccgccccc gcgttgtaca gttcaagcca
     7621 ttcgtatctc caccactgcc tttcttctac actctccact ggcaccccca tccaggctac
     7681 ttcgtttatt acaacttgag ctaaccccgg ggaaggaacc aaaaccaaga acaaaaccaa
     7741 acctattttc atacatcaac tatagcctta aatcctccca acctcaaccc tgacatgatt
     7801 aaatagcatg gtataatgta cttatctcat ctgagaaatt agtttgtttt gggctctctt
     7861 gcctgttcaa aacgagtaat aacagggcgg aatcgttcga gaaccggccc taaagatccc
     7921 aacccaacaa cttcttttgc gtcaatacgg tttgtactca ctcccggcgg aagaggcact
     7981 tcagaagaaa gctgaaacaa atgcgccaaa aatgtggtgg catacgctcc ctttggcaga
     8041 gaaaaagcga gcaccatcat attttctaag aataccgcct tatgaatctc cacctgctgc
     8101 aaggtagggg ctgtggggaa aatacccgct gagtatgaac gtaaaaacgg caaatctctg
     8161 tagagagatg tgggaagcgt aatgccgtct tgtttcaaga acttttcgta atgctcccaa
     8221 tctttggggt taccagaagt tacaaacgga agttcaaata gtatttcgcc ctccttaatc
     8281 gactgggaaa gttttttgtt aaaaaggtac gaagagtaag cgtatagcca aagtctcact
     8341 tgttctgcaa tggcgcgaag cgctccacgc aaatcgtttg ggcgttctac aagatattct
     8401 aaaaactgga gttctgttgg aaattgagac ggaaaagaac gcatttgttg atatatatat
     8461 ttccagttcc cccaatggtt ttctgtgtcc tttcgaatat gttgaaaata cattggctct
     8521 ctgccgcccc ggtagaaaag aagagttcgt attgcctgct catattcacc ccgcaataaa
     8581 tgccttccca taatatggcc gttaaatcgg ggggctgcaa aacgttgaaa cgaataaaaa
     8641 ttccaaaacc cttgttccga tatttcctta aggctttgct caatacgcag tttctctgct
     8701 gcttgcaagg gccctgcaaa gcgaatggta ataacgaacc tattccccca gagctggccc
     8761 tttttcaaaa cacctttacc gcgcacaata ttttttacaa aaaaatcctc gaacgcaaga
     8821 ttttctaaca actgcaggtt gcgcactttt cgcaaactta tcctttggct ggtaatggca
     8881 acttcgtctt ttaatccggc agtcccaata ctttgtgaag gaatttcaac aaatctggcg
     8941 atttcgtctt tagcatgaaa ggtagtaagg cctactttta ccaattccgc gtatatggtc
     9001 gacccttctt gctcttgcag attttgcacc ccagacccta tatcaactgt agaaattttt
     9061 ttatcgaggg aaatttcttc tacaataaaa tcttctggaa ggtattttaa gtgtccaaaa
     9121 gggtactccc caaacactcc ttcaatgcca atccattgca acagctggag tgtatctatc
     9181 ataggcggca tctcaaaaag ctcgggaacg cgctttttct ctgcaagcaa caactgatgc
     9241 tcttgttctt tttcttctcg tctcatatgg ccaatatact gcaattcctt gttttcgcaa
     9301 atataaagtc ccataactgg ctgtcatggg acttgcgaaa gatctacgaa ggtgctgagt
     9361 atggggtgac gagctagtcg agctcccaga gccctccggc aaaaccgagg gtgtgagacg
     9421 gacgctcgtc tgagcgatga cgggtattga ccgtgtggac gatgtaaccg tcttcgccaa
     9481 agcgattcgt ccagtcgtcg acggcgagtt cccgaatcaa ggtatctcta cccctcgggt
     9541 tagcccaaac agtggtatac ctccaccggc aacccagaca catattgaca cgctcccagg
     9601 cgaggtcaga cagctcgatc aacggcatgt ctagcatatt cgagaccacg agtgtcaata
     9661 tgtccatgcc cccggcgcgc cgctccgtga gttggacgtc aactagctga tatccgctct
     9721 tttccatttc ctcccaagcg gggagaacac agagcttctc cggatctttt tcgccaaaga
     9781 cccggacgcc cgcgtcagca acatgtctcc ggtcggtatc gtacaaataa ggatcccgac
     9841 caacccgttt ggcggttgcc acgtgctctt tctccaaagc ttcccggtcg agaacgatgc
     9901 gtcggatccc tgcctgctcg agtcgcttcg actgattcgg attaatcgtg aagcgaagca
     9961 tcacgatgtc gggcctagga gcctgggtgt aaacctcacc atgctctggg cactgccctt
    10021 cttgatttcg gcatcgggca tcgcccaagg gtgtagctcc gggacaagca taccactgct
    10081 cttcctttac tgtggtcatc acgacctcct tccatacttg aggcaatttg acgcctcatt
    10141 ttgtgtccat attatacact tatataagaa aaatgtcaat agtaagggca tgctaatctt
    10201 atctctcccc tcctaacccc agaatacgat aagtcttctg tattgaagta atagaaaaat
    10261 ttgatataat tacattgtta tgaagaagct ttcgaagaat tttttgtttg tggtcttaat
    10321 actcctttcc atttctgcgc tgtttgcgtt gctttcagat ccctttggcg caaccgagca
    10381 agttccgctc tcccaagttg tgcaagacat aaacgcagac caaatagaaa aaattgtagt
    10441 ttctggcaac aaacttgtca ttacttacaa agacgactca accaaagaat ccaccaaaga
    10501 agcacaagga gcattgcctg aaacattgat tgatttgggc gtacagcaag acaaactaaa
    10561 taccgtaaca attgaaccca gagaagaaag cgggtttgta acattgcttg gccccttaac
    10621 ttttatacta cttcccgtgc tgctgttctt cgtgtttttc tggatgatgt ttcgccaggc
    10681 aaggtctggc attggacagg cgttcgactt taccaaagcc agggcaaagt tatttggagc
    10741 ggaagggcag ttaaaacaaa aagtaagttt taaagacgtg gcggggttaa aagaagcaaa
    10801 agaagaagta caggaagtgg tggatttttt aaaattccca aaaaagtatt tgcaaatggg
    10861 agcaaaaatc ccccgtggag tattattggt aggacctccg ggtactggca aaacactcct
    10921 cgcgaaagct gtcgccagtg aagccggagt atcgttcttt tccgcttccg gttctgaatt
    10981 tatagaaatg tttgtagggg tcgggagctc gagagttcgg gatttattcc aaaccgcgaa
    11041 aaaatccggc cgctccatta ttttcataga cgaactcgac gccattggaa gaacgcgcgg
    11101 gtttgggttt ggcggcggac acgacgagcg ggaacaaacc ttaaaccaaa ttttagtgga
    11161 aatggacggt tttgagcgtg acgacacaca gatagtgatg ggggctacaa atcggcctga
    11221 cgtcctggat cccgcgctgc tccggcctgg cagatttgat cgcacgatta ttttggatct
    11281 tcccgatgta aacgaccggg aacaaatttt acaaattcac gcagaaggca agccgttagc
    11341 tccaaacgta aggctgcggg aaattgccga gcgcactccg ggattttcgg gagcggacct
    11401 ggcaaactta atgaacgaag ccgccatctt aaccgcgaga agaaacaagc agcaggtttc
    11461 ccaggcagaa ctgtttgagt ccatagacaa ggtgctcctt ggccccgagc gcaaaagcca
    11521 cgtactttcc aaaaaagaaa aggaagttac cgcctaccac gaagcggggc acgctttggt
    11581 tgcagcctcc cttcggcatg tagatccggt gcgcaaaatt tctattatct cgcgcggcag
    11641 agccggcggg tatacc
//
LOCUS       DASRUP010000103        15800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_432953, whole genome shotgun sequence.
ACCESSION   DASRUP010000103 DASRUP010000000
VERSION     DASRUP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15800
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432953"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFE94_04055"
     CDS             <1..209
                     /locus_tag="VFE94_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZX50283.1"
                     /translation="GFTSDLRQRVKEHKEGKGGRTTALKKHWKLIYAEAYIDKRDALG
                     REKFLKSGSGRRYLKKQMKHYLSI"
     gene            271..744
                     /locus_tag="VFE94_04060"
     CDS             271..744
                     /locus_tag="VFE94_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50284.1"
                     /translation="MFNFSRSQRKDITIARELNLLWNEICVVSERMRECNSDLVEDLY
                     KSFEGDGQFKNFKVAVLASAYTKLKEEKDLKGYLKELDAIRDKMAEKAHSVLDRYTNA
                     DNPWDAIKKELGEEKYDETLEAFYHDHFLTAQRFIIGAWLAKKNGDSLYMYKGPE"
     gene            748..1206
                     /locus_tag="VFE94_04065"
     CDS             748..1206
                     /locus_tag="VFE94_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50285.1"
                     /translation="MENQEFVKKQFEIFKKRGANYRNVLILTSFIETIIRENASKGKK
                     LKEFKQSLVILGLDIDPKDEKVVSYRKNSQHTNDCLIEINLLRVQRNELLHDILKEKH
                     PKEYIDKTIKEMARNLRSVCTSSNLIRDYFSKNTDYNFDPAKILATETGQ"
     gene            1458..1826
                     /gene="speD"
                     /locus_tag="VFE94_04070"
     CDS             1458..1826
                     /gene="speD"
                     /locus_tag="VFE94_04070"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HZX50286.1"
                     /translation="MATTKRTIKQSHLGTHIIAEFFGCKKLNDLKLIRQSLKEAAHIC
                     NATILHTKFHKFSPQGLTGYVLLAESHISIHTWPEHGYAAVDVFTCGLMDTEKAVRHL
                     ADQFEAQKVEFKKITRGKKR"
     gene            1823..2815
                     /locus_tag="VFE94_04075"
     CDS             1823..2815
                     /locus_tag="VFE94_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HZX50287.1"
                     /translation="MTLTRMQEKIIRTLLKTKGGNLDIWQLSRKCGYLSRVIQSVEQL
                     QEMGVVSAKDSLVRLTNKKLLPRYVVQKETPLKEVMKKFGVFRKEAIFTDNEYDQLPL
                     LAGAIEHKLSLMLRENDLLNRDIVCLGDDDIFSVACSLSGLPKSITVLEKDKRVIEFL
                     RKASQKVPVPIKVVQVDIARPLPERYHNSFDVCVSEPPDTVRGAFAFLSVGVQGLREG
                     GVLYIGISESALRNDQWLEMEQAVTKSGIRFTDIIQDFEEYALDGDEQFAWKGFEKLP
                     KWITKPAQKPWFVSVLLRGELVGAKNPTHFSLEGIGEDFLTNHLAAYQEELKPL"
     gene            2856..3584
                     /locus_tag="VFE94_04080"
     CDS             2856..3584
                     /locus_tag="VFE94_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZX50288.1"
                     /translation="MTLTNTEQQSEQKKPMPKKLIGVVMGVLVIGGAGYWAVSSGLLN
                     LGSFQTGVPSGALVAKVNGEGIEKRLFDARFGQAKSNYEVQGIALEAADAEQLRQEVL
                     DDMINKTLLAQYGIEQGIGAPDQAVEDSYRQIVTQFESEQELQDQLASQGITTEDVRS
                     AISQELIVSQVAERQAADNNVQVSEEETRKVYDDAVAGGAEVPEFDGVKGEIEQYVRQ
                     QKIGQLLNALVERLRAEGTVEILS"
     gene            3601..4155
                     /locus_tag="VFE94_04085"
     CDS             3601..4155
                     /locus_tag="VFE94_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZX50289.1"
                     /translation="MVLTKSEPKLKKGDKAPGFELQGVDGRQHTNRDGDFAGKEGLLV
                     IFMCNHCPYVKAKMPAIVELHKKFGERIGFIGINSNDPEYPGEGMENMKSFAAERDIA
                     FAYVLDNTQEVAKAYGATCTPDPFLFDREQKLVFHGRIDDALEPGDAVSEHTMEMKIE
                     QLLAGEEIADEFKPSMGCSIKWAS"
     gene            4591..5352
                     /locus_tag="VFE94_04090"
     CDS             4591..5352
                     /locus_tag="VFE94_04090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZX50290.1"
                     /translation="MLDTAIEAAKEAGEILKSYFETSLEKRVKDDKSIVTEADEKAEA
                     RIIEIIKTKYPDHGFLGEESGKQNAGAEYTWVIDPLDGTNNFVNGIPIFGTSIGLVKN
                     NTPILGVIYNPVTDTMFVAERGKGAFWNGHSIHVSEQHEDTAMIAIDTSSKPADKELV
                     KKCFVRSTEYVKIVRYLGSAVLGLGYLARGGTEGYINIGTNPWDYAAGSIIVLEAGGT
                     ITDFQGKPWDFSKNYFVASNGVIHAKLLELLKSVL"
     gene            5349..6164
                     /locus_tag="VFE94_04095"
     CDS             5349..6164
                     /locus_tag="VFE94_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50291.1"
                     /translation="MNDWKLFWILLVSCVVVVVAIWPIFFLLHGSVLEMLAVPHAILF
                     LLFLLKASLAFAGVVFFGLFMAERLGLGVPFLTAWLQGKQSKRLTHDVALMLFGAVVF
                     GLAAGIFVLVSDALFSLATGAVPGVGRMPVEIGDPATGMVRTIALSPLQGVLASLYAA
                     LSEEILLRLFGVSLLVWISQYIRGKGRVGKPKDAVVWGAIVSVALLSGFAHLPVVASF
                     PAMSLLMVARFALLGFTPAMLFGWLYWKKGLEAAVTAHFTSEFLLFVLIPLIA"
     gene            6169..6441
                     /locus_tag="VFE94_04100"
     CDS             6169..6441
                     /locus_tag="VFE94_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50292.1"
                     /translation="MPKKTTHYLTSKTEHTAHLVEKLVQEKRKAAFNRWPLLFTLLGA
                     FGLVATFYGFEGVMDQTGLSENPWILLALGIGVLIFTGSLYKKLGD"
     gene            6521..7480
                     /locus_tag="VFE94_04105"
     CDS             6521..7480
                     /locus_tag="VFE94_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50293.1"
                     /translation="MVNGLPLFPEEQELVRILQTERARCLWIVYAASPEIFSRYFPNL
                     INVRENLHPTRFLRVGRYTAGIIGVNTSIVAIPGFPGDFTRDELVFFMERIQAFALAV
                     GARGVALAGIIPGRFYRYGIPMDPPIVSGDLGAVYTIVETLQDALRMHGLALSTRPVR
                     VLGYGYIGQRCIEHLRQLGCTDLVAIERRLRGTYQRRGVIFTNSLDAIRQDDWVVFTA
                     GGGNEIDVARLPRIFGVDDMYPPFSVDQLAMFEAKESVIFQAALHAEGVVSNLPIEQF
                     AEDQFAGCMAQGLLQAAGVDTSRMTQGEFDITARARGIRSSLA"
     gene            7539..7808
                     /locus_tag="VFE94_04110"
     CDS             7539..7808
                     /locus_tag="VFE94_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50294.1"
                     /translation="MTRSEWLLSELRVAGLSVASLNDRIADHGMIGDPRWHEIRWIAE
                     SKYLGGNLIPDERFEALETFEDLLPLIAKLRQELQREKTRVLSTA"
     gene            7872..9491
                     /locus_tag="VFE94_04115"
     CDS             7872..9491
                     /locus_tag="VFE94_04115"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX50295.1"
                     /translation="MAAFLTFQFFAPLIAGIFAFVMGLAVLLVERKNVSYRYYFCTMA
                     AVAVWSVFVAVFHVPQVAHDIWTVLHWVAGIFIAPAYLLFLITFPSVEHVVKLPLRLL
                     IWGGAGLMAVAVLVWPENFVQYTVVDINEIPQLVVGPYVWILELFFPLYFLPGYFFAG
                     RKLWRAYGRERAHILTGLFGLFVATTGALITNVTLAIHFGREFIWLGPIFAAMAPASL
                     AYMLFGLREGRSRLFPSVALGGLVLLTLGYLTVTSETIEDFIFHAVLLVGVMLLAIFL
                     VRTSLLEAADVRKFQDLSRRLQRYNEELKQADRMKTEFLNIVSHHLRTPLTHIKWALT
                     ELAQGSYGGKLVPQQQKLTKELLANNERLVTFIESLMDTSKIETGQIVLHKEKVDLEK
                     LLWSTVKTFEKQAKAYYRVNIEKMPSTEKIPPLFVDKDAMRKVFENLLENALIYNKPG
                     GKIYAKTHMHNNFIEIEVTDTGIGIPAADLARVGQKFFRSPLAKRDEAEGTGLGIFIS
                     RHILRLHGGDLHVESQEGKGTTVLVKLPIVE"
     gene            9503..10639
                     /locus_tag="VFE94_04120"
     CDS             9503..10639
                     /locus_tag="VFE94_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZX50296.1"
                     /translation="MRELSAIKRIEMASRQIPGVVSLAQGTPSLYSDKAIRQAVIQAI
                     EENKVDKYSPGAGIPELRKEISRQLAREGMDYDAEGEILVTAGAIEALSAVFLALLQK
                     GNEVVVLTPTYYANYESIIEMAGGVAVEVQLEEQEGWQLDFEKLKAALTPQTKAVLLC
                     NPNNPTGSILSKEELTKLGQLAKERNLLLVFDDVYHHLVYGKELFCLATEGEYKDNII
                     RIVSFSKTFALTGWRIGFLHGPRELVAHIVPMHDNLLNCAPVVSQYAALAALQHEKEI
                     VPKVVEEYRKRRELMGGLLQEMKDYLSFVAPEGAYYFFPKIHGVANTMDFAFEVLEKA
                     KVAVVPGEDFGPGGEGHIRLCFGRAPEDIEEGLSRLKAYFEDLK"
     gene            10783..11223
                     /locus_tag="VFE94_04125"
     CDS             10783..11223
                     /locus_tag="VFE94_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50297.1"
                     /translation="MDTFPMELTPGEILYLSDHLSMFGQGPPDALPGQNSPYPQLLTK
                     ICSALLETVSTEKPTTVRMELSELWIIREVAKSNVMVGHEQVGLQLVGKVCAGILGLT
                     ANEYVEEAVKQWGEVAEDELSFEQAQATKTKGGGPNDESNNNPG"
     gene            11413..11817
                     /locus_tag="VFE94_04130"
     CDS             11413..11817
                     /locus_tag="VFE94_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50298.1"
                     /translation="MPGNKEDPKVKAFAWVEAENICKAWCVVDKWFQLMQTGEADNHL
                     PDPLWHRRDHGKKAPLTQELADKFIEELDEDFLIEKGRFGGRLPPDFSRLSPSGRIAF
                     VQEMYEVLLTEEELEEEADRRLKAKDIPVLAA"
     gene            11914..12417
                     /gene="idi"
                     /locus_tag="VFE94_04135"
     CDS             11914..12417
                     /gene="idi"
                     /locus_tag="VFE94_04135"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZX50299.1"
                     /translation="MKEQVTLVNEQDQPIGEGEKMQTHQEGKLHRSFSIFIFNSKGEV
                     LLQQRAKSKYHSGGLWSNAACGHPRPGEETLAAAHRRLQEEMGFDCELQELLQFTYKV
                     KLDHDLWEHEYDHVCKGKYDGQVHPNLEEVEDFEWVDKQTLLREVKRYPERYTEWFRI
                     VLPRTLS"
     gene            12494..13729
                     /locus_tag="VFE94_04140"
     CDS             12494..13729
                     /locus_tag="VFE94_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX50300.1"
                     /translation="MGSFNGQTVAIIGARGIGEAIADRCSKEGANLVLTYYEHADRLE
                     KVAEKATELGVNVLSLQFDITKAEDVDRLVAETTQRFGKADVLVLSAAGGLEPGKEDD
                     LEWPFVINCDAQVSLAKQASRHWPGIKIIYLTSLWAHCYGQVQMLPGYGSVAASKNAG
                     EAVLRTLDPTYSRVGIVCAHIVARTMVEGLFRRRVRQLHAELQATTPTGSFPSTEEVA
                     NAVIELASEKFAPGKTVYVGGDGSALPMLHDVSLGRTEIAQMLPMYGEDALYLDAFEY
                     NRKAKGAPATGMATLRIAEERCEGHFRDPYGKIFPAHKLMEAAAQGIGMLWLQTHIGT
                     EEFVGMYKRLGEASFEKPALPGDILRLEVKLMHECFSHFTGSCMVYNNSSGELVASIE
                     RVQLAMVPRGIAERMLRRS"
     gene            13731..14471
                     /gene="fabG"
                     /locus_tag="VFE94_04145"
     CDS             13731..14471
                     /gene="fabG"
                     /locus_tag="VFE94_04145"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZX50301.1"
                     /translation="MLEGKVALVTGASGGIGRAIALRLAREGVKVGLNYHTGKDPATQ
                     LMELIVSGGGEACLLQANVSQRKLVEQMVKELCEQWDRIDILVNNAGIHRDSLLLRMT
                     DEHWDDVLNVDLRGAFLCTNAVLRHMVSKRSGRIINISSIVGIKGNVGQANYAAAKAG
                     LIGFTKSAAQEYASRGITVNAIAPGFIDGTGMTAQLPAENRQGILDQIPAGRFGRPDE
                     VAGLVRFLCSEDAAYITGQVLTIDGGLT"
     gene            14490..15749
                     /gene="fabF"
                     /locus_tag="VFE94_04150"
     CDS             14490..15749
                     /gene="fabF"
                     /locus_tag="VFE94_04150"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZX50302.1"
                     /translation="MFTNKRVFITGVGFVSPLGLDVATNWANLKAGVSGVGPITLFDA
                     EGFTTTIAAEVKGFDPYNYMDRKEVGHADRFTWFAVAATQQAVAQAQLKIQDIDPYRF
                     GVVVSTGIGGIITLSQQHDVLRDRGPRRVSPFLLPMFLADMASGQVTRETGANGPNYC
                     LTSSCSSSADALIDVCRMILMGEIDIGIAGGTEAGICPIGVAGFSTMHALSRRNDDPQ
                     KASRPFDKDRDGFVMGEGSAILILESGESAASRGVQPWAEIIGYGQTSDAHHPTDPEE
                     TGQIAAAAMRFALDRAYIRPEEIDYFNAHGTSTPKNDIHETKALKLATGEAAFQIPIS
                     STKSMTGHMLGAGGSYEAGLCGLVMEHGVIPPTINLENPGEGCDLDYTPNKARHKEVN
                     VVMTASFGFGGHNSVLILAKPDWRDRR"
BASE COUNT         4238 a         3370 c         4280 g         3912 t
ORIGIN      
        1 caggttttac ttcggatttg agacaacgtg tgaaagaaca caaagaagga aaaggaggaa
       61 ggacaaccgc tctaaagaaa cactggaaat taatctatgc cgaggcatat atagataaac
      121 gtgatgcgct tggacgagaa aagtttctca aaagcggatc tggcagacga tacctcaaaa
      181 aacaaatgaa acattatttg agcatctgac caagacgagc taagaagtaa aaagtacgta
      241 gtgcccgttt tctgacgaaa gattagaatc atgttcaatt ttagtagatc acaacgaaaa
      301 gacattacta tcgccagaga gcttaatctt ctttggaacg aaatatgtgt ggtttctgaa
      361 cggatgcgag aatgcaatag tgatttagtt gaagatttat acaagtcttt cgaaggggac
      421 ggacagttca agaattttaa agttgccgtt cttgcaagtg cctacactaa attgaaggaa
      481 gaaaaagact taaaaggcta cctgaaggaa cttgatgcta tccgcgataa gatggcagaa
      541 aaagcacact ctgttttaga tcggtatacg aatgcagaca acccttggga cgctattaag
      601 aaggaactgg gagaagaaaa atacgatgaa accttggaag ctttctatca tgatcacttt
      661 ttgactgccc aaaggttcat aataggtgca tggctagcaa aaaagaatgg tgactctctc
      721 tacatgtaca aaggaccaga gtagcttatg gaaaatcaag aattcgtaaa gaaacagttt
      781 gaaatcttta aaaaacgggg cgcaaactat agaaacgttc taattcttac ttcgtttatt
      841 gaaaccatta tcagagagaa tgcgagcaag ggtaaaaaac ttaaagagtt caaacagtcc
      901 ttagtcattt tgggactaga cattgatcca aaagatgaaa aagttgttag ttacagaaaa
      961 aattcccagc atacaaatga ttgcttgatc gagatcaatc ttttgagagt tcaacggaat
     1021 gagttactgc atgatattct taaagaaaaa catcctaaag aatacataga taaaacgatt
     1081 aaagaaatgg cgagaaatct tagatcagtt tgtactagct ccaaccttat tcgtgattat
     1141 ttcagtaaaa atactgatta taactttgat ccggcgaaga tattagcgac cgagacgggt
     1201 cagtaagaca cccctggaaa gccctggaat ccctctcaaa accttggttc tgaaccgagg
     1261 atgccagcaa tacagcaaaa accttgactt tttggtcaaa tatggttata ctctttagca
     1321 atgctcatgt ctatgagtag cgaagaagat tggaattcta gaagctcaca gcttgccgcg
     1381 cggcaggaat ttatgcagcg cggatgggga gtattcgtgc ttccccggtt gagcctcttt
     1441 gcccgtatcc taagaatatg gcaaccacaa agaggacaat taagcaaagt catttgggaa
     1501 cccatattat tgcggaattt tttggttgca agaagcttaa cgatctcaag cttatcaggc
     1561 aatcgttaaa ggaggctgcg catatttgca acgccacgat tctccataca aaatttcata
     1621 agttttctcc ccaaggcctt accggctacg ttttgcttgc ggaatcccac atttccattc
     1681 atacatggcc cgaacacggc tatgcggctg tagacgtatt cacatgcggc cttatggata
     1741 ccgaaaaagc tgtacggcac ctggcggatc aatttgaagc ccaaaaagtg gaatttaaaa
     1801 aaataacaag aggcaaaaaa agatgacact tacccgcatg caagaaaaaa tcattcggac
     1861 tctcctgaaa acaaaaggag gcaatcttga tatttggcag ctttcaagaa aatgcgggta
     1921 tctttcgagg gttatacagt cggttgagca actgcaggaa atgggcgtgg taagcgccaa
     1981 agattcgctt gtgcgcctta cgaataaaaa actgcttcct cggtacgtag tccaaaaaga
     2041 aacaccgctc aaagaagtaa tgaagaaatt tggcgttttc cggaaagagg cgatcttcac
     2101 cgacaacgag tacgatcagc tccctcttct tgctggtgcc attgaacaca agctttccct
     2161 catgttgcgg gaaaatgatc ttttgaaccg cgatattgtt tgccttggcg acgacgacat
     2221 ttttagcgta gcttgttcgc tttccggttt gcccaagagc attacggtac tcgaaaagga
     2281 caagcgcgtg attgagtttt tgcgcaaagc atcccaaaaa gtgcctgtgc cgattaaagt
     2341 cgtacaagta gatatcgcgc gtccgttgcc tgaacgttac cataattctt ttgacgtttg
     2401 tgtttcagag cccccagata ccgtaagggg agcttttgca tttttatctg tcggcgttca
     2461 agggttaagg gaaggcggtg tactatacat tgggatatcg gaatccgcgc ttcggaacga
     2521 ccagtggctt gagatggagc aagccgtaac gaaatccggc atacggttta ccgatatcat
     2581 tcaggacttc gaggagtatg ctcttgacgg agatgagcaa ttcgcctgga aagggtttga
     2641 gaagttgccc aagtggatta cgaagcccgc tcaaaaacca tggtttgtat ctgttctttt
     2701 gcgcggtgag ctcgtaggag ctaaaaatcc cacacacttt tctcttgaag gcattgggga
     2761 ggatttctta acgaatcatc tggcggcata tcaagaagaa cttaagccct tgtaggggag
     2821 tttcttttaa gtagcatttt tgttatatta tagccatgac actcacaaat acagaacaac
     2881 aaagcgaaca aaagaagcca atgcccaaaa agctcattgg ggtagttatg ggcgttttgg
     2941 ttataggggg agccggttac tgggcagttt cgtctggctt gcttaacctt ggcagttttc
     3001 aaacgggcgt gccttccgga gcattggtag ccaaagtgaa tggcgagggc atcgaaaaac
     3061 ggctgttcga tgcgcgtttt gggcaggcaa aaagcaacta cgaggttcaa ggcatagcgc
     3121 tcgaagcggc tgatgcagaa cagctaaggc aagaggtgtt ggatgatatg attaacaaaa
     3181 cgcttctggc gcaatatggc atagagcaag ggataggcgc gccagatcaa gcggtggagg
     3241 atagttaccg gcaaatcgtt acgcagtttg agtcggaaca ggaattgcag gatcagctgg
     3301 cttcacaggg cattacaaca gaagatgtcc gctccgcaat ttcccaagag cttattgtaa
     3361 gccaggtcgc agaaaggcag gccgcagata ataacgtcca ggtttcagaa gaagaaacgc
     3421 gcaaggtgta cgacgacgcg gtggccgggg gagccgaggt gccggaattc gacggagtga
     3481 aaggggaaat tgagcaatac gtgcgccagc agaaaatcgg ccagttactg aacgcgcttg
     3541 tcgagagact tcgtgccgaa ggcactgttg agattttgag ctaacataat catatagcgt
     3601 atggtgctca ccaaatcaga gccaaaattg aaaaaagggg acaaggcgcc gggatttgag
     3661 ctgcagggag tagacggcag gcaacataca aatagagacg gcgactttgc gggcaaggag
     3721 gggctacttg taatttttat gtgcaaccat tgcccgtatg taaaggcaaa aatgccggcc
     3781 attgttgagc tgcacaaaaa atttggcgag cggatagggt ttattggaat caacagcaac
     3841 gatcctgaat acccgggcga aggcatggaa aacatgaaaa gttttgccgc cgaacgggac
     3901 attgcatttg catatgtgtt agacaacacg caggaagtag ccaaggcata cggcgccact
     3961 tgcactccag acccattttt attcgaccgg gagcaaaagc tggtgtttca tggaagaatc
     4021 gacgatgctt tggaacctgg cgacgcagtt tctgaacaca ctatggaaat gaaaattgag
     4081 cagctgctgg ctggggaaga aatcgcagac gagttcaaac cttccatggg ctgttctatt
     4141 aaatgggcct cataaaaggc aaaaggggtg ttatcataaa gacaacacac cctttcgtaa
     4201 ctgctacgtt agtttgtcgg tacattgagt gtactgacaa aaaacgttgc tttacttagg
     4261 agggtaagaa gaagttcctc gagtgcgtgg agcacatcgg ggagaaatac ccccgctacg
     4321 ataattaata ttgcgaaccc tatagctgct gccatagctt ttcgcaaagg agggggtctt
     4381 ataagcggac tgactaagtc cgcttccccg tttttatcgt gtggggcatc ttaccctcct
     4441 ttcttgaagc taagaacctc aagaaaggag ggtatagctg cgttttaaag agcagatgca
     4501 ttatagcaga aagcatattt ttgtcaaccc cggcaaaggt tttgttgata aaagttttgc
     4561 caggttttcc tgctgcggta taatatttgt atgttagaca cggcgattga agctgcaaaa
     4621 gaggcaggag aaatactcaa aagctatttt gaaacctcgt tagaaaagcg cgtaaaagac
     4681 gacaaaagca ttgttactga agccgacgaa aaagcggaag cccgcattat tgaaattatc
     4741 aaaacaaaat acccggatca tggatttttg ggagaggaaa gcggaaaaca aaatgcaggg
     4801 gctgaataca cgtgggttat tgatccttta gacggcacga acaactttgt aaacggcatc
     4861 cccatttttg gcacgtctat tgggttggta aaaaataata cgcccatttt gggagttatt
     4921 tacaacccgg taaccgacac catgtttgtc gcagagcggg ggaaaggagc tttttggaac
     4981 gggcatagta ttcacgtttc agaacaacac gaggatacgg ccatgattgc cattgatacg
     5041 tcttccaagc cagcagacaa agagctggta aaaaaatgct ttgtgcgttc taccgagtac
     5101 gtaaaaatag tgcggtatct ggggtctgcg gtgcttgggc tgggatattt ggcaaggggc
     5161 ggaacagaag gatatataaa tattggaact aacccctggg attacgctgc cggaagcatt
     5221 attgtgctag aggcaggagg aacgattact gattttcaag gtaagccgtg ggattttagc
     5281 aaaaactatt ttgtggcctc caatggagtt attcacgcaa aattgctaga attactaaaa
     5341 agcgtcttat gaatgactgg aaactttttt ggattctatt ggtttcgtgc gtagtagtgg
     5401 tagtcgctat ttggcccatt ttctttttgt tgcacgggag tgttcttgaa atgctggcag
     5461 ttccccacgc cattcttttc ttgctgtttc ttttgaaggc tagcctcgcg tttgccggtg
     5521 tggtgttttt tggacttttt atggcggagc gcctgggttt aggcgttcct tttctcacag
     5581 cgtggctcca ggggaaacaa agcaaacgcc ttacgcatga tgtggcgtta atgctttttg
     5641 gcgccgtggt gtttggactg gccgcgggca tttttgttct ggtaagcgac gcactgtttt
     5701 ctttggctac gggtgcggtt cccggagtag ggagaatgcc agtcgaaata ggggatccgg
     5761 caacgggaat ggtgagaacc atagcgctct ctccgctgca gggagttctt gcttctttgt
     5821 atgccgcctt aagcgaagaa atacttctcc ggctttttgg cgtaagtcta ttggtatgga
     5881 tttcgcaata cattcggggc aagggccgcg ttggaaagcc aaaagatgcg gttgtatggg
     5941 gggcaattgt gagtgtggcg ttgctttcgg ggtttgcgca tttgccggtg gttgcaagtt
     6001 ttcctgccat gagccttctt atggttgcgc gttttgccct gttgggtttt acccccgcca
     6061 tgctgtttgg ctggctttac tggaaaaaag ggctggaggc cgcagtgacc gcgcatttta
     6121 cttcggaatt tttgcttttt gtgttaattc cgcttattgc gtaatactat gccaaagaaa
     6181 acaacgcatt accttacgag caaaaccgaa cacacggctc atcttgtaga aaagcttgtg
     6241 caagaaaagc gcaaggccgc gtttaaccgc tggccgttgc tgtttacctt gcttggcgct
     6301 tttggtttgg tagccacatt ttacgggttt gaaggggtta tggatcaaac cggcctttcg
     6361 gaaaacccgt ggattttgct tgctctgggc attggcgttt taatttttac cggatctttg
     6421 tacaaaaaac tcggcgacta gcaaaattga caaaaaagca tttttctagt atgctattcc
     6481 ccagagcagc taagattttt ggttggaagg agaatgaagc atggtgaacg gcttacctct
     6541 tttccccgag gaacaggaac ttgtacgaat cctacagaca gaaagagcaa ggtgcctatg
     6601 gattgtgtat gccgcttccc cggaaatatt ctcaaggtat ttcccaaact taataaatgt
     6661 gagggaaaat ctacacccca ctcgattcct gcgagttgga cggtacacgg cgggaataat
     6721 aggggtaaat acctctatag tggcaattcc cggatttcct ggagatttta cccgagatga
     6781 gcttgtgttc tttatggaaa gaattcaggc gttcgctctt gccgtgggtg cacggggagt
     6841 tgcgttagcc ggcattatac ccgggcgatt ctaccgttac ggtattccta tggatccccc
     6901 gattgtaagt ggggacttgg gtgcggtata taccattgtc gaaacactac aggatgcatt
     6961 gcgtatgcat ggtttggcac tttcgacacg tcctgtccgc gtgctgggtt atggatatat
     7021 aggacagcga tgcatcgagc acttgcgcca gctcggctgt acggatctgg tggcaatcga
     7081 aaggcgattg cggggaacat accagcgccg cggcgttatc ttcacaaatt cgctcgatgc
     7141 tatccggcaa gacgactggg ttgtatttac tgccgggggc ggaaacgaaa tcgatgttgc
     7201 ccgccttccg cgaatattcg gcgtagacga catgtatccg ccgttttctg tggatcagct
     7261 ggcaatgttc gaggcgaaag agagcgttat attccaggcg gcgcttcatg ccgaaggagt
     7321 ggtatcgaat ctgccaattg aacagtttgc tgaagaccag tttgccgggt gcatggcgca
     7381 aggcttattg caagctgcgg gggtagacac gtcgcgtatg acccaggggg agttcgacat
     7441 tacggcgcga gcacgcggaa taaggtcaag cttggcataa aacgcttgac aattttcaaa
     7501 aatcactcac actggaagta agtactagga ggtggcccat gactcgaagt gaatggctct
     7561 tatcggagct gcgggtggcg gggttgtcgg tcgccagcct gaacgaccga atcgcagacc
     7621 acggtatgat cggcgaccct cgttggcacg agatccgctg gatagcggaa agtaagtacc
     7681 tcggagggaa ccttatacca gacgaaaggt ttgaagccct cgaaactttc gaggacttac
     7741 tccctctcat tgcgaagctg cgacaggaat tgcagcgcga aaagacaaga gtcctttcta
     7801 cggcatagcg ctttcttgaa tcttaccaag agaaagcgct gttttattta ttctatggta
     7861 tactattggg aatggcggcg tttttgactt ttcaattttt tgccccgctt attgctggta
     7921 tctttgcctt tgtaatgggt ttggcagtgc tccttgttga acgcaagaat gtcagttatc
     7981 gatattactt ttgcaccatg gctgcggttg ccgtgtggtc ggtgttcgtg gcggtgttcc
     8041 acgttcccca agtggcgcac gacatatgga ccgtgctgca ctgggtggcc ggcatattta
     8101 tagccccggc gtatctgttg ttccttatta cgtttccctc tgtcgagcat gtggtaaaac
     8161 tgcctttgcg ccttttgatt tgggggggag ccgggcttat ggccgtggcc gtattggtgt
     8221 ggccggaaaa ttttgtgcaa tacacggttg tcgacataaa tgaaataccg cagcttgtgg
     8281 tagggcctta tgtatggata ctggagcttt tctttccttt gtattttctg ccaggctatt
     8341 tctttgccgg cagaaagctt tggcgagcgt acggaagaga acgcgcgcat atccttaccg
     8401 ggcttttcgg gctttttgtt gctaccacgg gagctcttat taccaacgtt accctcgcca
     8461 tacactttgg aagagagttt atatggctgg gccctatttt tgcagccatg gctccagcga
     8521 gtttggcata tatgcttttt ggactgcgcg aagggcgctc ccgcctgttt ccttcggttg
     8581 cgctgggagg gcttgtgctg cttacgttag gatatcttac agttacgtcg gaaactattg
     8641 aggactttat tttccatgcc gtgttgctcg tgggggttat gttgcttgcc atatttttgg
     8701 tgcgaacgtc cctgctggaa gcggcagacg ttcgcaaatt ccaggattta tcgcgcaggc
     8761 tccagcgtta caacgaagag ttaaagcagg cagaccgcat gaaaacagaa tttctgaaca
     8821 ttgtttccca ccatttgcgc acgcccctaa cgcatattaa atgggcgtta accgaactgg
     8881 cgcaaggcag ctatggggga aagttggttc cccaacagca aaaactcacc aaagaacttt
     8941 tggcaaacaa cgagcggctc gttactttta ttgagtctct tatggatacg tcgaaaattg
     9001 aaaccgggca aatcgtgcta cacaaagaaa aagtcgattt ggaaaagctg ctttggagta
     9061 cggtaaagac atttgaaaaa caggcaaagg cgtactaccg tgtgaatatt gaaaaaatgc
     9121 cttcaaccga aaaaattccg cctctttttg tagacaaaga cgctatgcgc aaagtattcg
     9181 agaatctgct cgagaatgca ttaatctaca acaagccggg aggaaaaata tacgcaaaaa
     9241 cgcatatgca taacaatttt atagaaattg aagttaccga taccggcatt ggcattcctg
     9301 ctgcggattt ggcgcgggta gggcaaaagt ttttccgttc gcctttggcc aaacgcgacg
     9361 aggccgaagg caccggcctt ggcattttta tttcaaggca tattttacgg cttcacggcg
     9421 gcgatctcca tgtggagtcg caggaaggta aaggcacaac agttcttgtg aaactcccca
     9481 ttgtggagta gaattgtctg gtatgcgaga gctctcggca attaaaagaa ttgaaatggc
     9541 ctcccgccaa attccgggcg ttgtgtcttt agcgcagggg acgccttctt tgtattctga
     9601 caaagctatt cgccaggcgg taatccaggc aatcgaagaa aacaaggtag acaaatattc
     9661 tccgggagcc ggaattccgg agttgcgcaa ggaaataagc aggcagcttg cccgggaagg
     9721 tatggattac gatgcagaag gagaaattct tgttactgcg ggagccattg aggctttgtc
     9781 tgcggtattt ttggccttgc tccaaaaagg gaacgaagtt gtggtgctta cgcccacata
     9841 ctacgcaaac tacgagagta ttatcgaaat ggcaggaggc gtggcggttg aggtgcagct
     9901 tgaagaacag gaaggttggc agcttgattt tgaaaagctc aaagcggctt tgactccgca
     9961 aacaaaggca gtgcttttgt gcaaccccaa taatcctacg ggatccatac tttccaaaga
    10021 agaacttaca aaactcgggc agttggcgaa agagcgcaat ttgcttttgg tgttcgacga
    10081 cgtataccac catttggtgt atggaaagga gcttttctgc cttgcgacag aaggggagta
    10141 caaagacaat attatccgta ttgtgagctt ttctaaaacc ttcgcgctta cgggctggcg
    10201 cattgggttt ttgcacggcc ctcgagagtt ggttgcgcac atagtgccca tgcatgacaa
    10261 ccttttaaat tgcgcgcccg tggtatcgca atatgcagct cttgccgctt tgcagcacga
    10321 aaaggaaatt gtgccaaagg tagtggagga gtaccgcaaa agaagagagc tcatgggagg
    10381 tttgctgcag gaaatgaaag actatctttc atttgtggcg ccggaaggcg catattactt
    10441 ttttccgaaa atacacgggg tggccaacac catggatttt gcctttgaag ttttagagaa
    10501 agctaaagta gcggtggtgc cgggagagga ttttggcccg ggaggagaag gccacattcg
    10561 tttgtgcttt ggccgcgcgc cagaggatat tgaagaaggg ctttcgcggc ttaaagccta
    10621 ttttgaagat ttgaagtaat cggaaaagct ttttgtgttt gacagagaaa ggcggaaagg
    10681 tataatgagg caatcctcat taaggagtaa gcactttgat aaagtaaact tcggtcccaa
    10741 gccgctttac caaaatttta aaaaagggag gtggtgtgcg gcatggatac atttccaatg
    10801 gaattaactc caggggagat tctgtatctg tctgaccact tgtctatgtt cggacaaggg
    10861 ccacccgatg ccctgcctgg ccagaattct ccttacccgc agctgcttac taaaatatgt
    10921 agtgctcttt tggaaactgt gtcaacggag aagccaacaa ccgtacgcat ggaactttcc
    10981 gagctctgga taattcggga ggttgccaag tcaaatgtaa tggtgggaca cgaacaagtg
    11041 ggccttcagc tggtgggcaa ggtttgcgcg ggtattctcg gcttgactgc caacgaatat
    11101 gttgaagaag ccgtgaaaca atggggagag gttgcagaag acgaattaag ctttgaacag
    11161 gcacaggcca caaaaacgaa aggaggtggg ccgaatgacg agtccaataa caaccccggt
    11221 tagaactgat ccgataacaa ctccttggat cgagccccag ccggaacgct atgcgccaaa
    11281 cccggatcac tgccccagtc agcgaaaacg caccatcagg cgaattcggc gcgttatcga
    11341 gccataaagg agaagttgat ggcctacgag aacactggac caaaagagat ctatccccaa
    11401 tgcttgggcg atatgcccgg caacaaggaa gaccccaaag ttaaggcttt tgcgtgggtg
    11461 gaagccgaga acatctgcaa ggcatggtgc gtggtggaca aatggtttca gttaatgcag
    11521 acgggggagg ccgacaacca tctgcccgac cctctatggc atcgccgaga ccatggtaag
    11581 aaagcgccct taacgcagga gcttgccgat aagttcatcg aagagctcga cgaggacttt
    11641 ctcattgaga aaggcaggtt tggcggaagg cttccgccag acttctcgcg gttgtcgccc
    11701 agtgggcgca tagcctttgt gcaagaaatg tacgaggtcc tcctcaccga ggaggaactg
    11761 gaagaggaag cggacagaag gttgaaagca aaggacatcc ctgtgcttgc tgcctagagg
    11821 attgaattgt gtcgaaacaa tgggaggccc cacagcctcc ttttctatta cccatagttt
    11881 tcgggcggag gagtttatgg taactttata tatatgaaag agcaggtaac attggtaaac
    11941 gaacaagacc agcctatagg cgaaggtgag aaaatgcaaa cgcaccaaga gggcaagctg
    12001 caccgctcgt tctcgatttt tatattcaat tcgaaagggg aagtactctt gcagcagcgc
    12061 gccaaaagca agtatcattc ggggggattg tggagcaacg ccgcctgcgg gcacccaagg
    12121 cctggcgaag aaactctggc agccgcgcat cgaaggctgc aggaagaaat ggggtttgac
    12181 tgcgaattgc aggagctttt gcaatttacc tataaagtaa agctcgacca cgatttgtgg
    12241 gaacatgagt acgatcatgt atgcaaagga aaatacgatg gccaagtgca ccctaatctt
    12301 gaagaggtgg aagactttga atgggtagac aagcaaacgc ttttgcggga ggttaagcgc
    12361 taccccgaac gctacaccga gtggtttcgc atcgtgcttc ctcgcacctt gagttagttt
    12421 tccttgcgtg ttaggtagaa tatagctatc gcagacacaa aatttatctg cgagtaatac
    12481 gaaggagaat gagatgggct cattcaatgg acagaccgtg gcaatcattg gcgcaagagg
    12541 cattggagaa gcaattgctg accgttgttc caaggagggc gctaaccttg tacttacata
    12601 ctatgagcat gctgatcgct tagaaaaagt agccgaaaag gctacggagc ttggcgttaa
    12661 cgttctatcg cttcagttcg atatcacaaa agccgaggat gtagaccggc ttgtagcaga
    12721 aacgacccag cgatttggaa aggcagacgt tctggtcctc agcgccgcag gtggtttgga
    12781 gcctggcaag gaagatgatc tcgagtggcc ctttgtcatt aactgcgatg ctcaagttag
    12841 ccttgcaaaa caagcatcgc gacactggcc cgggataaag attatttacc taacgagtct
    12901 gtgggcgcat tgctatggcc aggtgcaaat gttgccgggt tacggatcgg tggcggcatc
    12961 taagaatgcc ggggaggcgg tgcttcgaac gctcgacccc acttactctc gggtaggcat
    13021 tgtatgtgcc catattgtgg cgcgcaccat ggttgaaggt ctatttcggc gcagagttcg
    13081 gcaactgcat gcagagcttc aggcgactac tccgactgga agtttcccga gcactgaaga
    13141 ggtggcaaac gcggtgattg agttggcaag cgagaaattt gccccgggca aaactgtata
    13201 cgtaggaggt gatggttctg cgctaccaat gttacacgat gtttcgctcg gccgcaccga
    13261 gatcgcacag atgcttccga tgtatgggga agatgctctc tatctggatg cgttcgagta
    13321 caaccgtaag gccaaaggcg cccctgcaac agggatggct acactacgta ttgcggagga
    13381 gagatgtgag ggacactttc gggatccgta tgggaaaata tttcctgcgc acaagctgat
    13441 ggaggcagcg gcccagggta tcggaatgct ttggttgcaa actcatatag gcacagaaga
    13501 atttgtcggc atgtataaac ggttgggcga ggcaagcttt gaaaagccag cacttcccgg
    13561 cgatattctg cggctggaag tgaagttgat gcatgagtgc ttctcgcact ttacgggttc
    13621 ttgtatggtc tacaataata gttctgggga acttgtagcg agcattgaac gcgtgcagct
    13681 tgcgatggtt ccccgtggga tagcagaacg aatgttaagg aggtcataga atgttggaag
    13741 gaaaggtggc tttggtaacc ggggcttcgg gcggcattgg acgagcgatt gcgctgcgtc
    13801 ttgcgcggga aggagtcaag gttgggctga actaccatac agggaaagat cccgctacgc
    13861 aacttatgga attaatagtg agcgggggtg gcgaagcctg tttgctgcag gcaaacgttt
    13921 ctcaaagaaa attggttgag caaatggtga aagagctctg cgaacagtgg gatcgcattg
    13981 atattctggt gaataacgcc ggtatacatc gcgacagctt acttttgcga atgaccgatg
    14041 aacattggga cgacgttttg aatgtcgacc tgcgcggcgc ttttctctgc acaaacgctg
    14101 tacttcgcca tatggtgagc aagcgaagcg ggcggataat taacatctcg tcgatagtag
    14161 gcattaaagg taacgtcggc caggcgaatt acgccgctgc gaaagccggg ctcatcggtt
    14221 ttaccaagtc agcagctcaa gagtacgctt cgcgcgggat tacggtaaac gccatagctc
    14281 ccggctttat cgacgggacc ggcatgaccg cgcagctacc cgcggagaat cgccagggca
    14341 ttctcgatca aattcccgcg ggaaggtttg gcaggccgga cgaagtcgct ggtcttgtgc
    14401 gatttctgtg cagcgaggat gccgcctaca taaccggaca ggttcttacc atagacggtg
    14461 gattaaccta gaaaggagaa gagggaaacg tgttcactaa caagagagtg ttcatcactg
    14521 gcgttgggtt tgtgagtccg cttggtttgg atgttgcaac caactgggca aatctcaaag
    14581 ctggagtttc tggcgtaggc cccattacac tctttgatgc agaggggttt actactacca
    14641 ttgcagctga agtgaagggg tttgaccctt acaactatat ggatagaaag gaggttggtc
    14701 acgccgatcg attcacttgg tttgcagtcg ctgcaaccca acaagctgtt gcacaagctc
    14761 agttgaagat tcaggatata gacccgtatc ggttcggggt ggtggtgagt actggtattg
    14821 gaggcatcat tacgctgtct caacagcatg atgttctcag agataggggg cctcgacgcg
    14881 taagcccttt cttgctgcct atgttccttg cagatatggc ttcgggccag gttaccaggg
    14941 aaacgggcgc caacgggccg aactactgtt taacctcttc gtgttcaagc agcgccgacg
    15001 cccttatcga cgtctgtcga atgattctaa tgggtgagat agatataggg atcgccggcg
    15061 gaaccgaagc cgggatatgc ccgattggtg tggccggctt cagtaccatg catgcgcttt
    15121 cgaggcgaaa cgacgatcct caaaaagcaa gccgtccatt cgacaaagac cgcgacggct
    15181 ttgttatggg ggaggggagt gcaatcctca tactggagag cggggagagc gcggcaagca
    15241 gaggcgtcca gccttgggcg gagataattg gatatggcca gacttcggat gctcaccatc
    15301 cgacggatcc cgaggaaact ggccaaatag ctgctgcggc aatgaggttt gctctggata
    15361 gggcatacat aagaccagag gaaattgact actttaacgc ccacggcacc tcgacgccca
    15421 aaaacgacat acatgaaacc aaagctctca agttagcgac aggggaggcg gcattccaga
    15481 ttcctataag ctcgacaaag tctatgactg gacatatgct gggcgccggt gggtcgtacg
    15541 aggccggact gtgcggcctt gtcatggaac acggcgttat tcctcccaca attaatctcg
    15601 agaaccctgg agaggggtgt gatttagatt acactccgaa taaagcacgc cacaaagaag
    15661 tgaatgtggt catgaccgca tcgtttggct tcggagggca taactccgta ctcatacttg
    15721 ccaagcccga ctggagagat aggcgatagg caagcaagca agcaagcaat agggatcctt
    15781 acgggctccc tgtttttttt
//
LOCUS       DASRUP010000104         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_2196395, whole genome shotgun sequence.
ACCESSION   DASRUP010000104 DASRUP010000000
VERSION     DASRUP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196395"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFE94_04155"
     CDS             complement(<1..398)
                     /locus_tag="VFE94_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZX50303.1"
                     /translation="MANELLADSDFFIALYKSDDANHEAALGMLEKIGEQGANLALSV
                     FAYSEIATVLSQRVGKNTARDFMKDAAISGVRILHMTDFLFEKAKETFLRQRSKNVSF
                     TDAGNIALAAAYGFDAIVSFDSDYKKNGLRL"
     gene            complement(388..642)
                     /locus_tag="VFE94_04160"
     CDS             complement(388..642)
                     /locus_tag="VFE94_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZX50304.1"
                     /translation="MRKVINISVSERLYKEVEEAVRQNKYETKSELFRDLLRAWKEGR
                     FGAPVGKFRADTLLKNAQKHAAKGGPSDLSQKHDHYLYGE"
     gene            complement(701..1420)
                     /locus_tag="VFE94_04165"
     CDS             complement(701..1420)
                     /locus_tag="VFE94_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZX50305.1"
                     /translation="MANSNITVIAVGGSLIVPHLSDTDGIDVLFLRAFRKMLLGEIKK
                     DRKFVLVAGGGKTARLYQKAARQVAKSENSSLDWIGIYAIYVNAHLLKILFGPDAHDE
                     ILTSDPSVQKSASIKKANKSVVISSGWKPGWSTDYDAIRLAEKFGTKDVIIAGDTPFV
                     FEKDPRKFPEAKPLPELTWSQYEKLVPKKWTPGLSSPVDPIATQLAKKLKLEVRLIRG
                     TDMKNFQNAIEGKKFTGTVIR"
     gene            complement(1551..2054)
                     /locus_tag="VFE94_04170"
     CDS             complement(1551..2054)
                     /locus_tag="VFE94_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50306.1"
                     /translation="MPHIDEIVRNITQLIGQIEEAKGAEGEVTATEAKLWEQLDPLGK
                     EVLEKLTPILQNAFALVGGHIEVLHRDRIQNFQYRFRLGKLGWAEEDRYTPSRYESAT
                     VKYVSSAWVSFLQEFERKGQYFVEQLQEKLEQVKSQKEMHEQTARLYEDLTKKVTCIL
                     HCLGEQE"
     gene            complement(2109..2993)
                     /locus_tag="VFE94_04175"
     CDS             complement(2109..2993)
                     /locus_tag="VFE94_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZX50307.1"
                     /translation="MRSQQVSFSQLMTYIRCPEHWLFRYRLGIRRQPRKVFKRGFALH
                     ETLQYHFEEKKKDGKGISLPEAKEFFVASFKNAMEDYQQELAEARPFLTREYLSKEKQ
                     VNVPDMVDASLKGLEVYFKQLNPYIKPDLIEQAFEFGVEPGLTVGGRIDLTDKSGVIH
                     ELKTSKVTPNKQDIRADAQIAIYQIGYQTITGKAPKGISKDYIVVSKTNPRIVRFKVV
                     HPFINKQSVLRNILNIMNAINNNVFYCLHPAESWICSKEWCPYYREHQQLKKLGFEAF
                     MAKYAPRYSSRKLARQRR"
BASE COUNT          737 a          786 c          655 g          932 t
ORIGIN      
        1 agtcgcaggc cgttcttttt gtagtcgcta tcaaagctca caatcgcatc aaaaccatag
       61 gcggcggcaa gagcgatatt gcccgcatcc gtaaaactca cattctttga acgctgccgc
      121 aaaaaggttt ctttggcttt ctcaaataga aaatcggtca tatgcagaat tcgcactccc
      181 gagattgccg catctttcat aaaatcgcgc gccgtgttct tccccaccct ttgggaaagc
      241 accgtagcaa tttcagagta cgcaaaaaca gaaagcgcaa gattcgctcc ctgttctcct
      301 atcttctcta acatgcccag ggccgcttca tggttggcgt catcgctctt gtagagcgcg
      361 ataaaaaagt cggaatcggc caatagctca ttcgccatac aggtaatgat cgtgcttttg
      421 ggaaagatcg gaagggccgc cctttgccgc gtgcttttgc gcgttcttta aaagcgtatc
      481 ggcgcgaaat tttcccacgg gcgcgccaaa ccgcccttct ttccacgccc gcagcaaatc
      541 gcgaaacaat tcgctttttg tttcgtattt attctgccgt accgcctcct caacttcttt
      601 atatagtcgt tcggaaacgg atatgttgat tacctttctc atatccgtag tgtaatactt
      661 tgtattacac cttgtcaata cctgcgccag aaacagttgc ttatcgaata actgttccgg
      721 taaatttctt tccttcgatt gcgttttgaa agtttttcat gtcggttccg cggatgagac
      781 gcacctcgag tttaagtttt ttggcaagct gcgttgcaat ggggtcaacc gggctggaaa
      841 gcccgggtgt ccattttttg ggcaccagtt tttcgtattg gctccacgta agctctggca
      901 aaggctttgc ttcggggaat ttgcgcggat ctttttcgaa tacaaacggc gtatcccccg
      961 cgataattac gtcttttgtg ccaaattttt ctgccaagcg aatggcgtcg taatccgtag
     1021 accagcccgg cttccaaccg cttgaaatca caacactttt gtttgccttt ttgatgcttg
     1081 ccgatttctg taccgaaggg tcgcttgtta gaatctcgtc gtgagcgtct ggcccaaaaa
     1141 gaatcttgag caaatgcgcg tttacataaa tggcgtaaat accgatccag tccagagagc
     1201 tattttctga ctttgcaact tgacgagctg ctttttgata caagcgggcg gttttgcccc
     1261 cgccggcaac cagcacaaac tttctgtctt tctttatttc ccccagcagc attttgcgaa
     1321 acgcgcgcaa aaacaacacg tcaatgccat ctgtatcgga caaatgcggc acaatcaacg
     1381 accccccaac cgctattacc gtaatatttg agttcgccat accttccgtt attctatgcc
     1441 ttctcgcctt atttgtccat attcccccca aaaataaaaa ggtactacga atttatgcat
     1501 gtgcacaaac ctatagcacc tttgaatgag cttcagtagc gctgttccta ctattcttgc
     1561 tctcccaagc agtgaaggat acaggtaact ttcttcgtga gatcttcata gagcctggcc
     1621 gtttgctcgt gcatctcctt ctgcgatttc acttgctcaa gcttttcttg gagctgctca
     1681 acaaaatact gcccctttct ttcgaattcc tgaagaaatg aaacccacgc gcttgagaca
     1741 tatttcactg tagcactctc gtaacgagac ggcgtatacc tgtcttcttc cgcccacccc
     1801 aatttaccga gtctaaatct gtactggaag ttttgtatcc gatcccgatg tagaacttct
     1861 atatgtccgc ccaccaaagc aaatgcgttc tgtagaatag gggtaagttt ttctaacacc
     1921 tctttgccga gaggatcaag ttgctcccat agtttcgctt ctgtggcggt aacttctcct
     1981 tctgctcctt tcgcctcttc aatttgtcca atgagctggg ttatattgcg tacaatctcg
     2041 tctatatgcg gcatagcttc cacctcttca ttcaggtaaa tttgagatta tctatatcct
     2101 aattattttt accgtctttg tctagcgagt tttcgagacg agtagcgtgg ggcgtatttg
     2161 gccataaagg cttcaaagcc gagttttttg agctgctggt gttccctgta atacgggcac
     2221 cactcttttg aacatatcca gctttcggcc ggatgcaggc aataaaacac gttgttattt
     2281 atggcattca ttatatttaa gatgtttctt aaaacagact gcttgtttat aaacgggtgc
     2341 accaccttga aacgaacaat gcgagggttg gttttagaaa ccactatata atctttggaa
     2401 attcctttgg gcgcttttcc ggtaatggtt tggtacccaa tttggtaaat ggcaatttgg
     2461 gcgtcggccc gaatatcctg tttgttgggc gtaactttgc tggttttgag ctcatgaata
     2521 accccgcttt tgtcggtaag gtctattctg ccccccaccg ttaggcccgg ttccacccca
     2581 aactcaaaag cttgttctat taagtctggt ttaatatacg ggtttaactg cttaaaatac
     2641 acttccaaac cttttaggct ggcgtctacc atgtctggca cgtttacctg cttttctttg
     2701 gaaaggtact ctcgcgttaa aaacggccgg gcttcggcaa gttcttgctg gtaatcttcc
     2761 atggcgtttt taaacgaagc cacaaaaaac tcttttgctt cgggcaaaga aatgcccttt
     2821 ccgtcctttt tcttttcttc aaaatggtat tgcaaagttt cgtgcagggc aaagccgcgc
     2881 ttaaacacct tgcgtggttg gcgccgaatc ccgagcctgt agcgaaacag ccagtgctcc
     2941 gggcacctaa tatacgtcat tagctggcta aaactcacct gttggcttcg catataggtt
     3001 tactataacg caccagtgcg actcttggca aaaggtaaaa attgtggttc aatcacccca
     3061 gaacacggag gtgaaaaatg ataataggaa acaggcgcgg cgacctcgac
//
LOCUS       DASRUP010000105         9028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_690303, whole genome shotgun sequence.
ACCESSION   DASRUP010000105 DASRUP010000000
VERSION     DASRUP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690303"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..344
                     /locus_tag="VFE94_04180"
     CDS             87..344
                     /locus_tag="VFE94_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HZX50308.1"
                     /translation="MNTTLTIKTDKKLRDTAKKTARELGVPLGTVMNALLKQFVRDRE
                     ITLSVRLPNAETRRAMRETQERKNVETFESFDQWKKGVQLP"
     gene            351..620
                     /locus_tag="VFE94_04185"
     CDS             351..620
                     /locus_tag="VFE94_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004163283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HZX50309.1"
                     /translation="MRRIVQHKRFRKDIKRMTRRGKNEQKLYEAVDMLVRLGYLPTKL
                     RPHRLVARWGGFWECHIEPDWLLIYDVSDVEVLLVRTGTHADLFE"
     gene            828..1832
                     /locus_tag="VFE94_04190"
     CDS             828..1832
                     /locus_tag="VFE94_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50310.1"
                     /translation="MSFFSSKPIRVISFTVFSSSILLTVATLFVAPVIAQNKQSFLTP
                     QQRQFTEQKQNARASQAKTPDHQVSGKGHTESVKPGAPVKQRTEKVRLTKPELQARWK
                     EEWESVQEELGHLGAEQAAQLRAQYSKTKEEIDNLPANRVVEIEIPVTEVGEHSIHFS
                     SFTYNSSGKKLDPINVIFYNVGSAWDVQFDLQNWTSIQWKTITDASTQWVYIWDKIHY
                     GGQDVWRKQDYQLAPVGSANAPWPRYHIRLFGSFTRDSHSPGFGAWTIGDAHHDDMWH
                     DCTDDWEVAEAMVRASFKDSNGNPMWFVGTIWQTDLGNAGNYQCAYNDGKGTLIELLY
                     "
     gene            1840..2760
                     /locus_tag="VFE94_04195"
     CDS             1840..2760
                     /locus_tag="VFE94_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50311.1"
                     /translation="MIRFLGIGILAFVALNFQLDKVFADETVELEVQNLQVPSFVRSG
                     ESIKIVGTVVNRGSTPVLNLKLGVAVAGADGPQKGWEVLEKDPQGVIAKLNPGDQIIF
                     SSKVRLGGDGLFQIGIVGTADNASLSPQAKTVRVIDSSTFALQTMLVLFFYLLLLGIG
                     AGSLWYICRPEENGKLLNPSVFQLGLGVTLATLGPPLLFFTMNLYIDAPLWGSNLFTL
                     AMMSIFGGGWLLTGSALRPQKSWRRGIFGAIILYVVLGIIWVALFNAGFSRGETPIIQ
                     TSDPFSLFLLVIAWPLQVAQFFGLFGLGFG"
     gene            2893..3204
                     /locus_tag="VFE94_04200"
     CDS             2893..3204
                     /locus_tag="VFE94_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50312.1"
                     /translation="MKIKLLFAVSLIVTVLTAWFLVQNTLYLLGVGFTLENIILNELP
                     QDYSTGDGSVFNMVIFVLWFGLPIVLALWGFTFVTFWKNRKSKRQNRDQKRAITLLKT
                     I"
     gene            3339..3722
                     /locus_tag="VFE94_04205"
     CDS             3339..3722
                     /locus_tag="VFE94_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50313.1"
                     /translation="MHLFPLNEKKPLLSANPQLLSLGIFVLTLGPFAIGLLRQTWESP
                     WTLNWLPFLMLILFVTGWFCVGAALRPRESSRKGLLLALISYLLVGGVWAIKIGGGVD
                     FTTPVVMLFWPMTMAQTLGVFGLSN"
     gene            4180..5388
                     /locus_tag="VFE94_04210"
     CDS             4180..5388
                     /locus_tag="VFE94_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50314.1"
                     /translation="MPFFNDKAIKILGGIFLIFLVLGVGIEILAGTFVGFSPFVEPLT
                     ALDRVGTYTAPYEEVEFYPENLMTDFAVQNGKLYISDFSSHLRVIDVSNPANPKEVAV
                     LPLEYQPRIAALTTEYLFLTRFPHLESDEKPEGKTDQIEIFDISNLKKVEKVGEYTPQ
                     CESFCLIFDSALVVDDTLYLPITERPFNGTAEKITIHVLDITNPSSVLKRTEIEIPRE
                     QPSEIRELQLQGSREYVGVVEEDRFHLFSQENAETYELASTYVLPQENRYTCRYFNDI
                     AIREKVAYLLEQYGASSGCGRAALRIFDISNPVSPRELGKFELRYNAGEIEVKGNYVF
                     VGNRARGMRVLDVSDPAAPKEIAHYTSFLTPLYQEIWQMEIDEEYAYVVGHQVLGDLK
                     RIDIIDISSL"
     gene            complement(5392..6123)
                     /locus_tag="VFE94_04215"
     CDS             complement(5392..6123)
                     /locus_tag="VFE94_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX50315.1"
                     /translation="MTQEIIPLELKKLGFKDKEVRVYLAGLELGVSSVQKIARKAGVV
                     RPTTYEIIRILKEKGLFAETTEKGRRFFVAQPPEKILHFLRVQKREIEEKEREFIRII
                     AVLESKYYSGERGGISMFHGEEGMRMLEEKVSFSSAPEILVFASRADKQEMQMREELY
                     KKIRLRLGKVQVKELYAEKLNGQFPYGERKSLATKSVVPGTLILFDQAAFFPAENFKG
                     YLIENQLVCELLKSFFHSLWEQIAK"
     gene            6269..>9028
                     /locus_tag="VFE94_04220"
     CDS             6269..>9028
                     /locus_tag="VFE94_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZX50316.1"
                     /translation="MPKERKQSKFGSYLSIIVLSAPAVLAGLFFYPAVSLAQEINPYY
                     GYERAPVQSQLVGRVNDFSGAFTNSFPLVVPPGRNGLQPDLALFYNSQEHNNASIFGY
                     GWSIGIPSIERANKKGSDKLYTENFFYSSLSGELEDISLSDGTHGTYGAKAEDGNFLK
                     YQYNTNNSWAVTDKKGTTYTFGATAASRQDDPAESTRAFKWMLTEVRDANNNFIRYEY
                     FKDAGQIYPSKIFYTGNGVADGIFEIEFLRELRSDNLKSYKTGFEVTTNHRISEIQAK
                     IQGAWVRKYVLNYAAGDNEVRSVLNSITESGQDESLNVITLPANNFDYQAKTPNWTQE
                     VGLGTPTPFITAFNEDNGVRIADVNGDGLADILRSVPTASSVYLHNGSSWTLDTNWSI
                     PLPFIGINGVDYGTRLVDINGDAIPDLVRAESGSPYKIFIQEGTTWNDYSSLWSFPEP
                     ITLSGSSDAGTRLADVNGDGLVDVLRSTDTYTKVHLHNGSGWTEDASWSIPESFVKEP
                     NSRDTGVRLADVNGDGLADILFHDGNIPSAKAYINNGAGWTEDLSWVPPVGFLDGNSK
                     DAGARLDDANGDGLIDILLSSDSVAEVYLNSGEGWVLDTSWATPEPFINDNNIDVGTR
                     LADLNGDGLVDVARNTGTIDRVYFAHPKNPDLLSHMDHSKGAKEDIAYKPSADLLNPD
                     LPFIVQTVETVTKDDSLGHLSTTAHEHTGGEYWSAEIFDRKLAGFAEVVKTDPAGNKT
                     KEFYHQGNTTNSSQGEFSDDVSKIGKVYRTEVTHSAGNKYAQTINKWENVDLGNQRDF
                     VKLTQKIDFSYDGNASHRDAAETYAYNDANGNLTQKVSWGEVTGSDNGTFVDAGNDKL
                     TTAISYASNGANVVGFPSQETTTNQAGSKVKETRNYYDGLALGSVDKGNLTKQEMWKS
                     STSYID"
BASE COUNT         2512 a         2026 c         2099 g         2391 t
ORIGIN      
        1 tacgtttttt gtagagctgc cgattcgtta aaacactctg ccgccgcggg gttgacagga
       61 cagcgtacta aaagtagtat aaaggtatga atacaaccct aaccatcaaa acagacaaaa
      121 agttaagaga cacggcaaag aagacggcaa gagagttggg agtgcctctt ggcaccgtca
      181 tgaatgctct gctcaagcag tttgtgcgcg acagagaaat tactctttct gtccgccttc
      241 ccaacgcgga gacgcggcgc gctatgcggg aaacgcagga acgcaagaat gttgagacgt
      301 ttgaatcctt tgatcagtgg aagaagggcg tgcaattgcc ataatcatct atgcgccgca
      361 ttgtccagca taagcgtttc cggaaagata tcaaaaggat gacgaggcgc ggcaagaatg
      421 aacaaaaact ctatgaagcg gttgacatgc ttgtacgcct tgggtacctc cctacgaagt
      481 tacgacccca cagattagtc gccaggtggg gcggattttg ggaatgccat attgagcccg
      541 attggttgct catctacgac gtaagcgacg tggaagtgtt gcttgtgcgt accggcaccc
      601 atgctgattt gtttgaataa tgtttgctca actttttaca ccgccgaaca attcgtcgaa
      661 gctcacggca acctggttaa gcaatcttct ctaactgtct ttttctaccg acatcggtgg
      721 tgcttttata ttctactgtg gataagtttt gtttatagtt attgacatac ttcctagccc
      781 atcatattat acatgtaaag gtttacaaat tttattttta aatgcatatg tcattctttt
      841 cctcgaaacc aattcgcgtc atttccttta ccgtcttttc gagtagcata cttttaacag
      901 tggctactct ttttgttgct cccgttattg ctcaaaataa acagtcgttc ctcaccccac
      961 aacagcgcca atttactgaa caaaaacaga atgctcgcgc ctctcaagca aaaacgcctg
     1021 accatcaagt aagcgggaaa ggacatacgg aaagtgtaaa acctggtgcg ccagtcaagc
     1081 agcgcacaga gaaggtaaga ttaacaaagc ctgaacttca agctcgatgg aaagaagaat
     1141 gggaatcagt gcaagaggaa ttgggacacc ttggcgcaga acaggcggca caattgcggg
     1201 cacaatattc caaaactaaa gaagaaattg ataatttgcc agcaaatcgc gtggtggaga
     1261 ttgaaatccc ggttacagaa gtaggggagc acagcattca tttttcttca tttacctata
     1321 attctagtgg caaaaaactc gaccccatca atgttatttt ctataatgta gggagcgcat
     1381 gggatgtaca gtttgatctc caaaactgga caagcataca atggaaaaca atcaccgatg
     1441 cgtctaccca atgggtatat atttgggata aaatacatta cggtgggcaa gatgtttggc
     1501 ggaaacaaga ctatcaacta gcacctgttg gatctgccaa cgctccgtgg ccacgctatc
     1561 atattcgctt gttcggctct tttacaaggg attcgcatag cccaggtttt ggggcttgga
     1621 ctataggaga tgctcatcac gatgatatgt ggcacgattg cactgatgat tgggaagtcg
     1681 ccgaagcaat ggtccgtgcc tcattcaagg actcgaatgg aaaccccatg tggttcgttg
     1741 gcacgatttg gcagaccgat ctgggaaatg cagggaacta ccagtgcgcc tataacgatg
     1801 gtaaaggaac tcttattgaa ttgctttatt aatttaaaga tgatacggtt tctgggaatt
     1861 ggaattcttg ctttcgttgc gctaaacttt caactagata aggttttcgc tgacgaaact
     1921 gttgagctcg aagtccaaaa tctccaagtt ccttctttcg ttcgatctgg tgaatcgata
     1981 aagattgtag gaactgttgt aaatagaggg agtacccccg ttttgaacct caagcttgga
     2041 gttgcggttg ccggcgcaga cgggccccaa aaaggttggg aggttcttga aaaggaccct
     2101 caaggcgtga ttgcaaaact taatcccgga gaccaaatta tattttcaag taaagtgagg
     2161 ctggggggtg acggtttgtt tcaaattgga attgtaggta cagctgacaa tgcatctctc
     2221 tccccgcaag caaaaacagt aagggttata gattcatcaa cttttgctct acaaactatg
     2281 ctggtattat ttttctatct tttgctactt gggataggag caggaagtct ttggtatata
     2341 tgcagaccgg aagaaaacgg caaactcttg aaccccagcg tatttcaatt gggtttaggg
     2401 gttactcttg cgaccttagg accacctctt ttgttcttca caatgaactt atatattgac
     2461 gctcctctct ggggctccaa cctattcacc ctcgcaatga tgagtatttt tggcggggga
     2521 tggcttttaa ctgggtctgc cctacggcca cagaaaagtt ggagacgagg catctttgga
     2581 gcaataattc tctatgtagt attaggtatt atatgggtgg ctctgtttaa cgctggtttt
     2641 agcagaggag aaacgccaat cattcaaaca tctgatcctt tctcgctttt cctcctggtt
     2701 attgcctggc cattgcaggt cgctcaattc ttcggattgt ttggtttagg gtttggctga
     2761 taaccgcccg cttgattcag tgggtcgcga gatcaagtgg catgcgcggt cgaacatgtg
     2821 gtatcctatt cacagcataa gagggagaag ccggaacttc tccctctcct actccaaaaa
     2881 attaaagaga ttatgaaaat aaaacttctt ttcgcagtta gccttattgt tactgtttta
     2941 accgcttggt ttctggtaca gaatacgctg tatctgttgg gagttgggtt tacgttggaa
     3001 aacattattc ttaatgaact gccgcaagac tacagtactg gcgatggttc ggtattcaat
     3061 atggttattt tcgtactttg gtttggattg cctattgtgc tcgctctttg gggatttact
     3121 tttgtaacct tttggaaaaa cagaaaaagt aaaagacaaa acagagatca aaaaagggcg
     3181 attacattac tcaaaacaat atgaataaaa tcgactggaa aacgcttttc tttgtcaccg
     3241 gcacggtctt ttttattctt tccattgccc aaactttgat taactgggta agtttttatt
     3301 ttctccgccc attttaggaa ttatagggac agctgataat gcatctcttt cccctcaatg
     3361 aaaagaaacc tctactaagc gctaatcctc aactgctaag tttaggtata tttgtattaa
     3421 cactggggcc gttcgcaatt gggcttctta gacaaacatg ggaatctcct tggacgctca
     3481 attggctacc ttttctcatg ctaattcttt ttgtaactgg ctggttttgc gtaggtgcgg
     3541 cattgcgtcc cagagagagc agccgcaaag gattactttt agctcttatc tcctatctat
     3601 tggtaggtgg agtatgggcg attaagattg ggggaggcgt tgattttacc acgcctgttg
     3661 taatgctatt ctggccaatg actatggctc aaaccctagg cgtgttcggc ttaagtaatt
     3721 aagagaaggg gagcgcgttt tatgtaaact tgccttctgt ctaattatac caacattccg
     3781 agcgatataa gtaagtgggt agaaaaagct tgcttcgcca aagacggctc tggtaataat
     3841 ggtatgtggc tctatctcac tgacgcagga tggatagctg tccatggaac agctcagggg
     3901 aaagtagctc gatggatcgt gaatcaccag tactggaaag gaacgcgaag ttcgtattcc
     3961 gtacagtcgg agattttcta ccatgccctc gagggtcgtc atgcaacgca ccccacgcac
     4021 attgagtatc attgctctgc gctaccatta tggcaaagac ttctttacgg ttgctgacct
     4081 tctcggtgta tgtgtggcgg ccccctactt atttatgggg gccgccgcta actttaaaca
     4141 gacttatgaa aacatttgtt ttatgataaa atcacataca tgccattctt caacgataaa
     4201 gccataaaaa ttctaggagg gatatttctt atctttttag tgctgggtgt tggcattgaa
     4261 attttagcgg gtacgtttgt tggattttca ccttttgtag agccgcttac cgctttggac
     4321 cgagtgggaa catataccgc tccatatgaa gaagttgaat tttacccgga aaaccttatg
     4381 actgacttcg cggtgcaaaa cggaaagctt tatatatcag attttagctc gcatctgcgc
     4441 gttattgatg tttcgaatcc cgcgaatccc aaagaggtcg ctgttcttcc tcttgaatat
     4501 cagccgcgca ttgccgctct caccacagaa tacttattcc tcacaaggtt tcctcatctc
     4561 gaatccgacg aaaaaccgga aggaaaaacc gatcaaatag agattttcga catatcgaat
     4621 ctcaaaaaag tagaaaaagt aggggagtat acgccacaat gcgaaagctt ctgtttaatt
     4681 tttgattcgg ctctcgttgt tgatgacacg ctttaccttc ccatcacaga aaggcccttc
     4741 aacggcactg ccgaaaaaat tactattcat gttctcgata ttacgaatcc cagctctgta
     4801 ttgaaacgca cggaaatcga gattccaaga gaacaacctt cagagataag agaacttcaa
     4861 ttacaaggaa gtcgcgaata cgtgggagtg gtggaagaag atcgtttcca cctcttttct
     4921 caagaaaacg ccgaaaccta tgaattggct tctacatacg tacttcccca agaaaaccgc
     4981 tatacctgcc ggtacttcaa tgacattgct atacgagaaa aagtagctta cctactcgaa
     5041 cagtacggtg caagctcggg gtgcggccgt gcagcactcc gtatatttga tatatcgaac
     5101 cctgtttctc caagagaact tggaaaattt gaacttcggt ataacgcagg agaaatcgag
     5161 gtaaaaggta attatgtatt tgtgggtaat cgtgcccgag ggatgcgtgt tctggatgta
     5221 tcagatcctg cggcacccaa agaaatagcc cactacacaa gtttccttac gcctctttat
     5281 caagaaatat ggcaaatgga aattgacgaa gagtacgctt acgtggtggg acaccaggta
     5341 ttgggggatt taaagcgcat tgacattata gatatttcaa gcctatagct cttactttgc
     5401 gatctgctcc cacaagctat gaaagaacga tttgagaagc tcacagacga gctggttttc
     5461 gataagatag cccttgaagt tttctgcagg gaagaacgcg gcctggtcga aaaggataag
     5521 cgtgcccgga accacgctct ttgtggcaag ggattttcgc tcgccgtagg ggaactgccc
     5581 gttgagtttt tctgcataca actctttcac ctgtactttt cccaaacgca agcggatttt
     5641 tttgtacagt tcttcgcgca tttgcatttc ctgcttgtcg gcgcgggaag caaacaccag
     5701 aatttccggg gcagaagaaa acgaaacctt ttcttcgagc atgcgcatgc cttcttcgcc
     5761 atgaaacatg ctaatgcctc cgcgctcgcc cgaataatat ttggactcca acacggcgat
     5821 aatgcggata aactcgcgtt ctttctcttc gatttcgcgc ttttgcacgc gaagaaaatg
     5881 caaaatcttt tcgggcggtt gagccacaaa aaaccgcctg cctttttccg tggtttcggc
     5941 aaacaggccc ttctctttga gaatgcggat aatctcgtaa gtggtaggcc gcactacgcc
     6001 tgctttgcgc gcgattttct gcaccgaaga aacccccaat tccaaacccg caaggtacac
     6061 cctaacctct ttgtctttaa accccagctt tttgagctct aagggtataa tttcctgcgt
     6121 cattacaaac agtatagcat tctttcgaga gacgtcaaag tgtcggcacc tcttgacaac
     6181 cattcttgca taccctaggg tggaaagtag ggaagacgat aaggtaaaag tcagcatgtc
     6241 attataaacc gactataaca atatctctat gccaaaggaa cgaaaacaat cgaagttcgg
     6301 gtcgtatctt tccataatag tattaagcgc gccggctgtt ttagctggct tgttttttta
     6361 cccggcagtt tcgctggccc aggaaataaa cccttattac ggctacgaac gagcgcctgt
     6421 gcaaagccag ctggtgggca gggtaaacga cttttcggga gcttttacca actccttccc
     6481 tctggttgtt ccgcccggcc gcaatggact tcagccagac cttgcgcttt tctacaatag
     6541 ccaggagcac aacaacgcca gtatttttgg ctatgggtgg agtatcggca taccttctat
     6601 agaaagagcg aataaaaagg ggagcgacaa actctacacc gaaaactttt tttactcatc
     6661 gctttctggt gaactcgaag atatttcttt gagcgatggc acgcacggca catacggcgc
     6721 aaaagcggaa gacgggaact ttttgaaata ccaatacaat accaataact cctgggcggt
     6781 aacagacaag aaggggacca cttacacctt tggcgcaaca gcagcctcgc gccaagacga
     6841 tcctgcagaa agcacccgtg cattcaagtg gatgcttacc gaagtgcgcg atgccaacaa
     6901 caactttatt cgctacgaat actttaaaga cgccgggcaa atatacccct caaaaatttt
     6961 ctacacgggc aacggcgttg ccgacggcat ttttgaaatt gaatttctcc gcgaactccg
     7021 aagcgataac ctgaagtcgt acaaaaccgg ttttgaggtt actacgaacc accgtatttc
     7081 tgaaattcag gcaaaaatac aaggagcgtg ggtaagaaag tatgtgctca actatgcagc
     7141 aggggacaac gaagttcgtt ctgtgctgaa ctcgattacc gaaagtgggc aggacgagtc
     7201 gctaaatgtt attacccttc ccgctaataa cttcgactac caagcaaaaa cgccgaactg
     7261 gacccaagaa gtaggtctag ggacgccaac accctttatc actgccttta acgaggacaa
     7321 cggagtaaga atagccgatg taaatgggga cgggcttgcg gatatccttc gcagcgttcc
     7381 tacagcctca agcgtatacc ttcacaatgg ttctagctgg acactggata cgaactggtc
     7441 tattccactt ccgtttattg gcataaacgg tgttgattat ggaactcgac tggtcgatat
     7501 aaacggagat gcaattccag atctggtaag ggcagaatcc ggaagccctt acaagatatt
     7561 tattcaggaa ggtactactt ggaatgacta ttctagcctg tggagctttc ccgagccaat
     7621 taccttatcg ggttcgagtg atgccggaac gcgtttggca gacgtaaacg gcgacggctt
     7681 agttgacgtg ctgcgctcca ccgacaccta taccaaagta catctgcata acggttctgg
     7741 gtggacagaa gacgccagtt ggagcatacc cgagtctttt gttaaagaac ccaatagcag
     7801 agatactggt gtacgccttg cagatgtgaa cggcgacgga ctcgcagata ttctcttcca
     7861 tgatggaaat atcccaagcg ccaaagctta tatcaacaat ggggcgggtt ggacagagga
     7921 cttaagctgg gtccctcccg taggatttct cgatggcaat tcgaaagatg ctggagcccg
     7981 cctcgacgat gccaacggcg acggcctcat agatatcctt cttagttcag acagtgttgc
     8041 agaagtctat cttaattcgg gtgaagggtg ggtgctggat acttcctggg ccacgccaga
     8101 accctttata aatgataaca acattgatgt tggaacacgc ctagcagact tgaatggcga
     8161 cggccttgtt gatgtagccc gcaacacagg aaccatagac cgcgtctact ttgctcatcc
     8221 aaaaaatcct gatcttcttt cgcacatgga tcacagcaag ggggccaagg aagacattgc
     8281 ctataagcct tctgccgatc tgctaaatcc agacttgcca tttattgttc aaaccgtaga
     8341 aaccgttacc aaagacgaca gtttgggcca cctttccaca acagcgcacg aacatacggg
     8401 tggggagtac tggagcgccg aaatcttcga cagaaaatta gctggttttg cagaggttgt
     8461 aaaaacagat cctgcaggga ataaaacaaa agagttttat catcaaggca atactacaaa
     8521 ttccagtcag ggcgagtttt cagatgatgt atcgaagatt ggcaaggtat accgcaccga
     8581 ggtgacacac tccgcaggca acaagtatgc ccaaacaatt aacaaatggg agaatgttga
     8641 tttgggcaac cagcgcgact ttgtaaagct tacccagaaa atcgactttt cttacgacgg
     8701 aaacgcgagc caccgggatg cggcagaaac ctatgcctac aacgatgcaa acggaaatct
     8761 cacgcaaaaa gtgagctggg gcgaagttac gggaagcgac aacggaactt tcgttgatgc
     8821 cgggaacgac aaactcacca ctgctatttc gtatgcaagc aatggagcaa atgtcgttgg
     8881 ctttccctcg caggaaacaa cgactaatca ggcaggctcg aaagtaaaag aaacaaggaa
     8941 ttactacgat ggattggctt tgggcagtgt agataagggc aacctcacaa agcaggaaat
     9001 gtggaaatcg agcacctcgt atattgat
//
LOCUS       DASRUP010000106        10409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_124385, whole genome shotgun sequence.
ACCESSION   DASRUP010000106 DASRUP010000000
VERSION     DASRUP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124385"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFE94_04225"
     CDS             <1..348
                     /locus_tag="VFE94_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZX50317.1"
                     /translation="KAALKEMFLPTALVVISPIAVGFLLGPEALGGFLMGTIITGLFV
                     AISMTSGGGAWDNAKKYIEEGHFGGKGSDAHKAAVTGDTVGDPYKDTAGPAINPLIKV
                     VNIIAILIARFLV"
     gene            389..1051
                     /locus_tag="VFE94_04230"
     CDS             389..1051
                     /locus_tag="VFE94_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZX50318.1"
                     /translation="MISLASETRKKEEKVASLRKQGFIPGVLYGPKTNPSTVKVDAKE
                     FSKAYKEAGESSLVNLKSQETTVPVLIREIQKEPVRGAVIHVDFYQPPLDEEIQVAVP
                     LVFQGEAPGVKDLGGTLLRNIHEVEVKALPQNLPHEIVVDVSKLTTFEERILVRDLAH
                     DANVEILRDPEEVVAQVVKAEDVEAELLQPVIENVEEVESSKPEKKEEEEGAEAPEEK
                     PE"
     gene            1090..2451
                     /locus_tag="VFE94_04235"
     CDS             1090..2451
                     /locus_tag="VFE94_04235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HZX50319.1"
                     /translation="MQRISKALIPFVIVSVFALPGFLIFAQQGENLTPTEERDLLEQE
                     LKQLEDQIKGIEGDITKTQKEKDTLKNQIAVLRNKIKKLNLQVEQSNKIIVDLRSQIS
                     DTTSSIDKTTGDIEGLKDEMAAVLQRVYREDQRSKVEIVLASSTLSDFFNDMAQLVFL
                     NAQLEELLGDMKELHGYLETQKDALVDEKGEEENFVKIQLLQKQENQNIQVQTEQLLV
                     QTQGKEVEYQRLLADKKQRAQQIRSRIFDLIGVPDAPTFGEAVLIAKGVAVQTGVRPA
                     LLLAVLTQESNLGKNVGQCYLNNTATGAGVSVKGTPFSNVMKPTRDVQPFLTITKDLG
                     RDPFQTPVSCPIPSVGGYGGAMGPAQFIPSTWMGYKKRLDAILARPADPWNIRDAFLA
                     SGLYLGDSGATDHSYNAEWCAAQRYFSGRCSTSYRFYGDSVMSLAARYEKDIQTLDEV
                     NGI"
     gene            complement(2448..3485)
                     /gene="prfA"
                     /locus_tag="VFE94_04240"
     CDS             complement(2448..3485)
                     /gene="prfA"
                     /locus_tag="VFE94_04240"
                     /inference="COORDINATES: protein motif:HMM:NF001859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZX50320.1"
                     /translation="MDLAQAKKEYTEISEKLTDPELISKWDEFQELSKRRGVLEKIVK
                     KALLVEELKERIAENNQIIASQEDELASLAQEELSSLAQRKEQAQKELEELLRDESQD
                     IPDAIIMEIRPGTGGEEASLFAGDLFRMYTRYAEQKGFKYTILEMDDTELGGIKTASV
                     EFNGKEAVRKLLHEAGVHRVQRIPSTEKAGRIHTSTASVAILPKPKKAQVQLNPSELK
                     VDIYNASGPGGQNVNKRKTAVRITHLPTGLVVASQASRNQQKNRDFAMSLLAAKILQQ
                     REETAGAAIAGQRKTQIGSADRSEKIRTYNFPQDRITDHRVKESWHGVERILKGDLDE
                     IGDTLSQNQNL"
     gene            complement(3486..3788)
                     /gene="rpmE"
                     /locus_tag="VFE94_04245"
     CDS             complement(3486..3788)
                     /gene="rpmE"
                     /locus_tag="VFE94_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZX50321.1"
                     /translation="MKKDLHPTYYPNAKVTCACGNTFTVGSTQESLQVEVCSNCHPFY
                     TGKDKVMDKVGRIQKFKERMAQKQDTPRKAKTKKAKTQAVSRTQGKARVTSKKTKA"
     gene            3894..4280
                     /locus_tag="VFE94_04250"
     CDS             3894..4280
                     /locus_tag="VFE94_04250"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX50322.1"
                     /translation="MNKEFIQSTLQGFFDNEKEVLFAYLFGSHATGHAIAKSDVDIAV
                     YLNPKNKQDQFEKRLELIAALTKLLKKEVDVIVLNMASPFLKHVVFKEGTLVFDRDAS
                     SRIDFDLKSLNEYFDYKLQWSALGMA"
     gene            4336..5073
                     /gene="rpsB"
                     /locus_tag="VFE94_04255"
     CDS             4336..5073
                     /gene="rpsB"
                     /locus_tag="VFE94_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZX50323.1"
                     /translation="MKDTDAAEAGKDPVSPTGGDKNLEEMMEAGLHFGHKTSKTHPKM
                     KPYITGVRNTIHIINLEKTKEKLDEALLALAEMVALGKVVVFVGTKVQIKNFVHDTAV
                     EANVPYVTERWIGGLLTNFEGVSKRIAHLKDLEKKLADEEEANKYTKWERHEMQEEIE
                     KLNEKFGGMKTLEKLPDAIFVFDFDENQLAVKEAKQKGIQVFAICDTNCDPSLVDYAI
                     PANDDAISSVRYILQKVKDAILKARGT"
     gene            5070..5522
                     /gene="tsf"
                     /locus_tag="VFE94_04260"
     CDS             5070..5522
                     /gene="tsf"
                     /locus_tag="VFE94_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZX50324.1"
                     /translation="MKPTVEQIKELRELTGISIAECKKALEEAGGNAEKAKALLKEWG
                     KEVAAKKQERATGEGLVVSYIHGTGKVGSLVAVKCETDFVARSDDFKNLCHELALQIA
                     SMEAENVEELLSQEYIKDSGKTIADVINEAIAKLGENIVVDRFVRFRI"
     gene            5531..5875
                     /locus_tag="VFE94_04265"
     CDS             5531..5875
                     /locus_tag="VFE94_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50325.1"
                     /translation="MSQLILLALTLGSLGVIVFIVYRKLPKLRELSAQELQGQETPIA
                     RARAIVSSRVNSQKITRMALTQARSLAEKTEAQTGEWLERLQKNTQARKGEFTQSYWE
                     QLRKKARVRKKK"
     gene            5882..5953
                     /locus_tag="VFE94_04270"
     tRNA            5882..5953
                     /locus_tag="VFE94_04270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5914..5916,aa:Thr,seq:tgt)
     gene            5955..6764
                     /locus_tag="VFE94_04275"
     CDS             5955..6764
                     /locus_tag="VFE94_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50326.1"
                     /translation="MKVFVLVVFIALVVFVLINLPIVTGRGVKAVKEALANTQPIELS
                     GKTLFISDLHLTAEPSLEQQFNLDFSNVQNVVIVGDFFNQAEDFESFGATEEESMRAV
                     LERIAPQAFSGTIFFIASGHSHDPRLQEISRLRFENFEFIYLGEYGKFILGGEQEVVA
                     MHGGQLHEGIIGGGVTWLAGKFGLVLPLEKLGKNRFGIDKNTWLVAGHSHVPGIDQAA
                     KVANTGSFVGAPFNKFIFRIHIGTGVLFDGGAVELQEYEGLNLKELYLFAL"
     gene            6774..7634
                     /locus_tag="VFE94_04280"
     CDS             6774..7634
                     /locus_tag="VFE94_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX50327.1"
                     /translation="MAVLLLALGSAFFIGLNNVLTRKALDFTSRGQAILVSLGVATTI
                     FLVLNAVLGKIPLWFVPGALLFFLGGLFGPGIGRTFNVISAKRIGVSRTIPVVGTAPF
                     FATILAILFLGEEYSFYIFLGMAFIVFGIFVLSRGKKENGKRAFDKKDLLLPLGSAVC
                     GGVSIVVSKVALGILDDPIVGVGLTMVGALLVVLGYIVGTRRVRHFRVMHRGSIFPVL
                     GGFCMAAAFFLNFSALQIGDVSIVAPVFSTFPLFGVFLSHFFLKEEITGRTWLGAIII
                     VLGIAIIQAF"
     gene            7725..8483
                     /locus_tag="VFE94_04285"
     CDS             7725..8483
                     /locus_tag="VFE94_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HZX50328.1"
                     /translation="MLETHNLSFWYGDRRREKQVLFDISIGFPTNKITALVGPSGCGK
                     TTLLKCFNRMHEIGNTVKFRIDGKILFNGQDLYRADLPVEQVRYLAGMVFQRPNPFPK
                     TIYANVAFGPDINGFRGSMDEIVEEALRRADLWEEVKDRLHGRALSLSGGQQQRLCIA
                     RALALEPPVLLLDEPCSALDPIATLHIEDLLREIAYDTTIIIVTHNIGQARRLSDYMA
                     FLCEGELIEFSETRLMSTNPRDERTEAFLMGRLG"
     gene            8596..9066
                     /locus_tag="VFE94_04290"
     CDS             8596..9066
                     /locus_tag="VFE94_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50329.1"
                     /translation="MKNALRIVSLLLLGLVIGCVEAPVIAKTVTPVPELSAEFDRASV
                     RPNSPVDVRLGEDVYLYIEFTNTGAERAEFCAAVTLRPVGEMTNRNDINLRPLIPTAL
                     DPGERGVAEWRYKTTDVGNFDLIFGLWGACAAELEPNHPVVHSGWFENYIRVSP"
     gene            9494..>10409
                     /locus_tag="VFE94_04295"
     rRNA            9494..>10409
                     /locus_tag="VFE94_04295"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2692 a         2184 c         2852 g         2681 t
ORIGIN      
        1 aaagctgcct taaaagaaat gtttttgcct accgctcttg tggtaatttc gcctattgcc
       61 gttggttttt tactggggcc tgaagccctg ggagggtttt taatgggaac gattatcacc
      121 ggtttgtttg tggcaatatc catgacctcg ggaggagggg cgtgggataa cgccaaaaaa
      181 tatatcgaag aggggcattt tggcggcaaa ggatcagacg cccacaaagc tgcggttacg
      241 ggcgacacag tgggggatcc ctataaagat acggcaggcc ctgccattaa ccccctgatt
      301 aaggtggtaa atattattgc cattctcatt gcccgttttt tggtctagct ccttgcaaaa
      361 ttctgccaaa tgggtataat gggtagcgat gatatcttta gcctctgaaa cgagaaaaaa
      421 ggaagaaaag gtggccagct tgcgaaaaca gggatttatc ccgggtgttt tgtatggccc
      481 caaaactaat ccttcgactg tgaaggtgga cgccaaagaa ttttccaagg cgtataaaga
      541 agcaggcgag agctcgctgg taaacttaaa gtcacaggaa acaacggtgc ccgttttaat
      601 cagagagatc caaaaggaac cggtgagggg tgctgttatc catgtggatt tttaccagcc
      661 gcctttagat gaagaaattc aggtggcagt tcccctggtg ttccagggag aagctcccgg
      721 agtaaaagac ttgggaggta ccttgttgcg caatattcac gaagtggaag tgaaagctct
      781 gcctcaaaac cttccgcatg aaattgtggt ggatgtttca aagcttacta cgttcgaaga
      841 gcgcattttg gttcgcgatt tggcgcacga cgccaacgta gaaattcttc gggatcctga
      901 agaagtcgta gcccaggtag taaaggcaga agacgtagaa gcagagctcc tgcagcctgt
      961 gatagaaaac gtagaagagg tggaatcttc aaaaccagag aagaaagaag aggaagaagg
     1021 agcagaggca cccgaggaaa agccagagta aatttacaac ctcaatggta ggccgagttc
     1081 ggcggcttta tgcaaagaat ttcaaaagcg ttgatacctt tcgttattgt ctcggttttt
     1141 gcattgccgg gctttttgat ttttgcgcag cagggagaaa atcttactcc caccgaggaa
     1201 cgcgatttgt tggagcagga gttaaagcaa ctggaagatc agattaaggg catcgaggga
     1261 gacatcacca aaacccagaa agaaaaagat accttaaaga atcaaattgc agttttgagg
     1321 aacaagatta aaaaactcaa cctgcaagtt gagcaaagca acaagattat cgtcgacttg
     1381 cgctcgcaaa tttctgatac tacgagttct atcgataaaa cgacagggga tatagaaggc
     1441 ttaaaagatg aaatggccgc ggtgctccag cgcgtgtatc gagaagacca gcggagcaaa
     1501 gtggaaatcg ttttggcgag ttcaacactc tctgactttt ttaacgatat ggcccagctc
     1561 gtctttttaa atgcgcaact cgaagagctg ctgggagaca tgaaagaatt gcatgggtat
     1621 ttggaaacgc agaaagatgc gctggtagac gaaaagggcg aagaggaaaa ttttgtaaaa
     1681 attcagctac tgcaaaagca ggagaaccaa aacattcagg tgcaaaccga gcagctctta
     1741 gtgcaaacac agggcaagga agtcgagtac cagcggcttt tagccgacaa aaagcagcgc
     1801 gcccagcaaa ttcgctcgcg tatttttgat ttaatcggcg tgccagatgc tcctaccttt
     1861 ggggaagcgg tacttattgc aaaaggagtg gcggtgcaaa cgggagtgcg gcccgcgttg
     1921 ctgcttgcgg tgctcacgca agagtcgaat ttgggcaaaa atgtcggcca gtgctatttg
     1981 aacaacaccg cgacgggagc cggagtaagc gtaaaaggaa cgcctttttc caatgtaatg
     2041 aaacctacgc gcgacgtgca gcccttctta accataacta aagatttggg gcgcgatcct
     2101 ttccaaacgc cggtttcctg tccgattccg agtgtgggag ggtatggcgg tgccatgggt
     2161 ccagcccagt ttattccttc cacgtggatg ggatacaaaa agcggcttga tgccattttg
     2221 gcacgaccgg ccgatccctg gaacattaga gatgcgtttt tggcttcggg gttgtacctg
     2281 ggagattctg gcgctaccga ccacagctac aacgctgaat ggtgcgcggc gcaacggtac
     2341 ttctcgggaa ggtgctccac cagctatcgc ttttatggcg actcggtaat gagcctggcc
     2401 gcccgctacg aaaaagacat tcaaacctta gacgaagtaa acgggattta gagattttgg
     2461 ttttgggaaa gcgtgtcgcc gatttcgtct aaatcgccct tgagaatgcg ctcgacgccg
     2521 tgccacgatt cttttacgcg gtggtcggtt atacggtctt gaggaaagtt gtatgtccgg
     2581 attttctctg aacgatcggc cgagccaatc tgggtttttc tctggccagc gattgcggct
     2641 cccgccgttt cttctctttg ttgcaggatt tttgccgcaa gcagcgacat ggcaaaatcg
     2701 cggtttttct gctgattgcg cgaggcctgg ctggccacca ccagtccggt aggcaagtgc
     2761 gttatgcgca cggcggtttt gcgtttgttt acgttttgcc cgcccgggcc cgaagcgtta
     2821 taaatatcaa ccttgagctc tgaagggttc aactgaacct gggctttctt gggctttggc
     2881 aaaatggcaa ccgaagccgt agacgtgtga atgcggcctg ctttttctgt cgagggaatt
     2941 cgctgtaccc ggtgcacgcc cgcttcgtgc aaaagtttgc gtaccgcctc tttcccgtta
     3001 aattctacag atgctgtttt tattccgccc agctccgtat cgtccatttc cagaatagtg
     3061 tacttgaagc ctttttgttc ggcgtatctg gtgtacatgc ggaataggtc gcccgcaaac
     3121 aaagaggctt cctcgccgcc cgtgcccggg cgaatttcca taataatggc gtcgggaatg
     3181 tcttgtgact cgtctcgcaa gagctcttcc aactcttttt gcgcctgttc tttcctttgc
     3241 gccaaagagc ttaattcctc ctgcgcaagc gaggctaatt catcttcctg cgaggcgata
     3301 atctggttgt tttccgcaat gcgttctttg agttcttcta ccagcagggc tttctttacg
     3361 attttttcca aaacaccccg gcgcttggaa agttcctgaa attcgtccca tttggaaatt
     3421 aactcagggt cggtgagctt ttctgaaatc tccgtatatt ctttttttgc ctgtgcgagg
     3481 tccacttaag cctttgtttt cttgctggtt acccgtgctt ttccttgagt gcgtgaaaca
     3541 gcctgggttt tagctttctt tgttttcgct tttctgggag tgtcttgctt ttgagccatt
     3601 cgttctttga atttctgaat gcggcctacc ttgtccatta ctttgtcttt tccggtgtaa
     3661 aagggatggc agtttgagca aacctcaacc tgcagagact cttgcgtcga acccacggta
     3721 aacgtgttcc cgcacgcaca ggttactttg gcattagggt aatatgtcgg atgtaagtcc
     3781 tttttcatag cactggcatt ataccccaga acagcaaaag aatcaagggc ttgcggggga
     3841 tgccgcaagg aatggcggtt tttttgtttt tgtggtaaaa agggtgcatg gaaatgaata
     3901 aggagtttat acaaagtacg ttgcagggct tctttgacaa cgaaaaagaa gtgctttttg
     3961 catacctttt tggttctcat gcaaccggac atgcgatagc aaaaagtgat gtggatattg
     4021 ccgtatattt gaacccaaaa aacaagcaag accaattcga aaagcggctt gagttgattg
     4081 cagcattaac aaaactgctc aagaaagaag tggatgttat tgtgctcaat atggcttcgc
     4141 cgtttttaaa acacgtagtg tttaaagagg ggactcttgt ttttgataga gacgcgagct
     4201 cgcgcattga ttttgatctt aagtctctta atgagtattt tgattataag ttgcaatggt
     4261 cggccttagg aatggcttag gtattgcgtt ctggccgatt ttgtgataaa aaagatgggt
     4321 atggaagaaa cacttatgaa ggacacggat gccgcggagg cgggaaagga tcctgtttca
     4381 ccgacgggcg gagacaaaaa cctcgaggaa atgatggagg ctggtttgca ttttgggcac
     4441 aagacctcaa aaacgcatcc caagatgaag ccgtatatta cgggcgtcag aaacaccatt
     4501 catattatta atttggagaa aaccaaagag aagctcgatg aggcgctttt ggcgcttgcg
     4561 gaaatggtgg ctttgggtaa agtagtggtg ttcgttggca ccaaggtgca gattaaaaac
     4621 tttgtgcacg acacggcagt ggaggcaaac gtaccctatg ttaccgaacg gtggatcggc
     4681 ggattgctta ccaacttcga gggtgtttcc aagcgcattg cgcacctgaa agatttggag
     4741 aaaaagctgg cagatgagga agaagcgaac aaatatacaa aatgggagcg ccatgaaatg
     4801 caagaagaaa tcgaaaaact caacgagaaa ttcggcggca tgaaaacact ggaaaaactg
     4861 cccgacgcca tatttgtttt tgacttcgac gaaaaccagc ttgccgtgaa agaggcaaag
     4921 caaaaaggca ttcaggtatt tgctatttgt gacaccaact gcgacccaag ccttgtggat
     4981 tacgccattc cggccaacga cgacgccatc agttctgtcc ggtatatttt gcaaaaagta
     5041 aaagatgcga tactcaaagc aagaggaaca tgaagccaac cgtagagcaa attaaagaac
     5101 tccgcgaact cacgggcatc tctattgccg agtgcaaaaa agcgcttgag gaagcgggcg
     5161 gaaacgcgga aaaggcaaaa gcccttctga aagagtgggg gaaagaggtt gcagcaaaaa
     5221 aacaagaaag agcaacgggc gagggcttgg tggtttcgta tattcatggt acgggtaaag
     5281 tagggtcttt ggtggcagta aaatgtgaaa ccgattttgt ggcgcgttcc gatgatttta
     5341 aaaatctttg ccatgaatta gctttgcaga tagcctcgat ggaagcagaa aacgtagaag
     5401 agttgctgtc gcaagagtac atcaaagatt cggggaaaac gatcgcagat gtaatcaatg
     5461 aggctatcgc caaactcggg gagaacattg tggtagaccg gtttgtgcga ttccgcattt
     5521 agttttggta atgagtcaac ttatactttt ggcccttaca ctgggcagtc tgggtgttat
     5581 tgtttttatc gtgtatcgga aattgcccaa gctgcgagaa ctttcggcgc aagagcttca
     5641 gggccaggaa acgcccatag cccgtgcacg ggcaattgtg agttctcgtg taaactccca
     5701 gaaaattacc cgtatggcct taacccaggc gcggagcctt gccgaaaaaa ccgaggctca
     5761 aacaggggaa tggttagaga ggctccagaa gaatacgcag gcaagaaaag gggaatttac
     5821 gcaatcctat tgggagcaac ttcgcaaaaa agcccgcgtg cgcaagaaaa aataactctt
     5881 tgccagcgta gctcaatggc agagcagctg ttttgtaaac agcaggttgt cggttcgagc
     5941 ccgaccgctg gctcatgaaa gttttcgttt tggtcgtttt tattgcgctt gttgtttttg
     6001 tgttgataaa tcttccaata gttactggca ggggggtcaa agcagtaaaa gaagcactgg
     6061 caaacacaca gcccatcgag ctttccggta aaacactgtt tatttccgac ctgcacttaa
     6121 cggcagaacc ctccttggaa caacaattta atttggattt cagtaacgtg cagaatgtgg
     6181 taatcgtagg agactttttt aaccaggccg aggatttcga aagttttggc gctaccgaag
     6241 aagaaagcat gcgagcagtg ttagagcgta tagcgccgca agcgttttcg ggaacaatct
     6301 tttttatcgc aagcgggcat tctcacgacc ctcgcttgca agagatttcg cggttaagat
     6361 ttgaaaactt cgagtttatc tatttgggag aatatggaaa atttattctt ggtggggagc
     6421 aagaggtagt cgcaatgcat ggagggcagt tgcacgaggg aatcataggc gggggagtta
     6481 cctggctggc gggaaagttt gggcttgtgc tgccgttaga aaagctgggg aaaaatcgtt
     6541 tcggcatcga caaaaacact tggcttgtag cagggcatag ccatgtgccg ggtatcgacc
     6601 aggcggcaaa agtggcaaac acgggatcct ttgtgggagc tccctttaac aagtttattt
     6661 ttcgcataca tattggtaca ggagttttgt tcgatggcgg cgcggtggag cttcaggaat
     6721 acgaggggct aaacttaaaa gagctgtatc tctttgcgct ctagcaatat cttatggcag
     6781 tacttttgct tgctttaggc agcgcgtttt ttatagggtt aaataacgtg cttaccagaa
     6841 aggcgctgga ctttacttcg cgaggccagg cgattttggt ttctcttggc gttgccacaa
     6901 cgattttttt ggttctgaac gccgtacttg gaaaaatacc cctatggttt gtgcccggag
     6961 ctttattatt ttttttgggc ggactgtttg gccccggcat tggcaggacg tttaatgtta
     7021 tttcggcaaa acgcattggc gtgtcgcgga ccatacccgt agtgggaact gcgccgtttt
     7081 ttgccaccat attggcaatt ttgtttttag gcgaagagta ctcgttttac atatttttgg
     7141 gaatggcgtt tatcgtattt ggaatttttg ttttgagccg cggcaaaaaa gaaaacggta
     7201 agcgcgcgtt cgacaaaaaa gatttgctct tgcccttggg atctgccgtg tgcgggggag
     7261 tgtccattgt ggtttccaaa gtggcattgg gtatattaga cgaccctatt gtgggagtag
     7321 ggcttactat ggtgggcgct ttgcttgtgg tgttggggta tattgtgggc accaggcgcg
     7381 tccggcattt tcgcgttatg cataggggat ccatatttcc ggtgcttggg ggattttgca
     7441 tggccgcggc atttttctta aacttttccg ctttgcaaat tggcgacgtg tccatagtgg
     7501 cgccggtgtt tagcacgttt ccgctgtttg gcgtgttctt aagccacttt ttcctaaaag
     7561 aagaaataac cggtcgtacc tggctgggcg ccattatcat tgtccttggc atcgccatca
     7621 tccaagcgtt ttgatagatg aaaagctttg tgataaggtg tcagtagaaa gttcaagctg
     7681 gagggaaaac agttttgaat actgcatttc agggaagttc tcccatgctg gaaacgcaca
     7741 atttgagttt ctggtatgga gatagacgca gagaaaagca agttcttttc gacatatcaa
     7801 ttgggtttcc tacaaacaaa attacagccc tggtgggtcc gagtggctgt ggcaagacta
     7861 cgttgctgaa atgttttaac cgaatgcatg aaatcggtaa cactgttaag tttcgcatcg
     7921 atgggaaaat tttgttcaac gggcaagacc tatacagagc agatttgccg gttgagcagg
     7981 tgcgttattt agctggtatg gttttccaac ggccaaaccc tttcccaaaa acaatctacg
     8041 ccaatgttgc gtttgggccc gatatcaatg ggtttcgtgg cagtatggat gagattgtgg
     8101 aagaggccct ccgccgcgcc gatttgtggg aagaggtaaa agatcggctc catgggcgag
     8161 cattgagttt gtcgggtggc caacaacaac gtttgtgtat tgcccgtgcc ttagcacttg
     8221 aaccaccggt gcttttgctc gacgaaccgt gttctgcgct agatcccata gctacgcttc
     8281 atatagaaga cttgctgcgg gaaatagcgt atgatactac cataataatc gttacccaca
     8341 acattgggca ggcacggcgc ttatcagact atatggcgtt tctgtgcgag ggcgagttga
     8401 tcgagtttag tgaaaccagg ttgatgagta caaacccgag agacgagcga acagaggctt
     8461 ttctcatggg tcgtttagga taacagtctc gcatacaagg cgccacgttt tattaacgtg
     8521 ctttggtacg ggaggggtaa tttggtattt cttgggtagc aactttggcg aaaagttttt
     8581 gtggaggatg gtgaaatgaa gaacgccttg agaattgtaa gcctgctact tcttggattg
     8641 gtgattggtt gtgtagaggc tccggtaatc gccaagaccg ttacgcctgt tcccgagctt
     8701 agtgcggaat tcgaccgcgc gagcgtacgg ccaaacagtc ccgttgatgt aaggttaggg
     8761 gaggatgttt acctgtatat agagttcacg aatacgggtg ctgaaagagc ggaattttgc
     8821 gcggcggtaa cactgcgccc ggttggtgag atgactaata ggaacgacat taacctgcgc
     8881 ccgttgatcc ctactgccct tgatcctggc gaaaggggag tggccgaatg gagatacaaa
     8941 accactgacg tcggaaattt tgatctaatt tttgggcttt ggggtgcgtg cgctgccgaa
     9001 ctagaaccaa accatcctgt tgtgcactct gggtggtttg aaaactatat cagggtgtct
     9061 ccctagtagt cagtagaacg aagaatcttc gggttcctcg gcctactttt acaagagctt
     9121 tggttatccg ccagtttacg gacccagagc tttttgattc gcaaaataat aaagagcgtc
     9181 tttttatggg gcttgacatt ggtttgaggg gggagtaaga tacggggtgc gagggagagc
     9241 cttgtaaaac cctaaaacca tgaactagca aatctgtaaa aacgttacac gcgtaagaca
     9301 gatcccgaaa gtactcgaag gcccgaatac tttcggggtt tgttttcccc gcccaaggcg
     9361 ggcaggggag aggcgaacca gaactttgac aagttcatag acgagacaat aaatttaccc
     9421 cgcgatgaaa ttgcaaattt cattttgcgg ggaccaacgt taattttttt gagttgcttc
     9481 gactcttaag tttttatttg agagtttgat cctagctcag ggtgaacgct ggcggcgtgg
     9541 ataaggcatg caagtcgagc gagccccgca aggggcgagc ggcaaacgag ttagtaacac
     9601 gtgggtaacc acccatcaca cgggcataac ccggagaaat ccgggctaat tcccgatggc
     9661 tcccgtaagg gtaaaggagt aatccggtgg tggaggtgcc tgcggcctat cagcttgttg
     9721 gtgaggtaac ggctcaccaa ggctatgacg ggtaggggac gtgagagcgt gttccccaac
     9781 gatggcactg cgatacgagc catacaccta cgggtggcag cagtcgagaa tattccacaa
     9841 tgggcgaaag cctgatggag cgacgccgcg tgcgggatga aggccttcgg gtcgtaaacc
     9901 gcttttatcg gggaagaatc cgcaaggatg acggtacccg atgaataagg gggtgctaat
     9961 tctgtgccag cagccgcggt aaaacagaac cctcaagcgt tacccggatt tattgggcgt
    10021 aaagcgtctg tagccggttt ggcaagtctt aggttaaata ccaagagctc aacttttggg
    10081 gtgcttgaga aactgccaga ctagagggcg ttaggggctg atggaacgca cggtggaggg
    10141 gtgaaatccg ttgatatcgt gcggaacacc aaaagcgaag gcattcagct ggggcgaccc
    10201 tgacggtgag agacgaaagc gtggggagcg aaaaggatta gatacccttg tagtccacgc
    10261 tgtaaacaat ggatactggc tatgtgaagt gtcgaccctt cacgtggcga agctaacgcg
    10321 ttaagtatcc cgcctgggaa gtacggccgc aaggctaaaa ctcaaaggaa tagacggggg
    10381 cccacacaag cggtggatca tgcggctta
//
LOCUS       DASRUP010000107        12845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1555861, whole genome shotgun sequence.
ACCESSION   DASRUP010000107 DASRUP010000000
VERSION     DASRUP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12845
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555861"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFE94_04300"
     CDS             complement(<1..539)
                     /locus_tag="VFE94_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002830501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZX50330.1"
                     /translation="MKVKKRVIVAMSGGVDSSVSAALLKKAGFDVTGMFMKCWSAQDP
                     FGENCTATDDERMARLAAAKIGIPFYSINLIKEYRQRVVQYLLDGYKKGITPNPDIMC
                     NKEIKFGLFFDKAMELGVDYVATGHYARVHKGSIFMAKDQNKDQSYFLSFIKPEVLDK
                     VMFPIGDYTKPEVRKLAKK"
     gene            645..1160
                     /locus_tag="VFE94_04305"
     CDS             645..1160
                     /locus_tag="VFE94_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50331.1"
                     /translation="MNYFAGAIVFLVGFSIAIAVLLGCIAINKYLKGKSYRKIDVNVL
                     ALLVTGTIPMGLTVASWEVFQGLRPYFLYASLLWGITFGLSSFVWLCISLMSPIFYLG
                     GRLKDSWLDFWFQLLDPAGWENRFAFPYGRRLRTLRQSAKAGHFDLEQLPFVMWVNKQ
                     LGTGPGSKGKK"
     gene            complement(1201..1704)
                     /locus_tag="VFE94_04310"
     CDS             complement(1201..1704)
                     /locus_tag="VFE94_04310"
                     /inference="COORDINATES: protein motif:HMM:NF016215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HZX50332.1"
                     /translation="MEYFLNEKFFNPTKGSLYLQEVLEEMVAYMKETPHKKYEVIVGC
                     DSSATEDPTFPVVIVVLRKQQGGRFFLRRVHYESRPFPSRRQRIMEEVMLSCQLALWL
                     RENFTPLAMANGLEYNFQYIHADIGEKGITRDMVKEVTGLIRGNGFEPKIKPYSFAAS
                     TVADRFS"
     gene            complement(1717..2394)
                     /gene="tpiA"
                     /locus_tag="VFE94_04315"
     CDS             complement(1717..2394)
                     /gene="tpiA"
                     /locus_tag="VFE94_04315"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZX50333.1"
                     /translation="MDKPIIIANWKMNPATAREAQELFEKVAEGVKGIDGAEIVICPP
                     FVYIPDIVRAASSPALSVGGQDCFWEEKGAFTGEISPQMLKEAGCSHVILGHSERAKY
                     LGETEDMVQKKVQAALAAGLKVILCIGENEQTPQDAPNLIVVYEPEWAISTEGGKAAN
                     AGRVAARVVQMRQTLKTSPILYGGSVDSLNIGAFLKEAGVQGVLVGSASLDAKEFITI
                     ARNAAMQ"
     gene            complement(2387..3013)
                     /locus_tag="VFE94_04320"
     CDS             complement(2387..3013)
                     /locus_tag="VFE94_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZX50334.1"
                     /translation="MEHLLELLASMVMYSIEATGYAGIALLMAAESANIPVPSEIIMP
                     FAGYLVSKGEFSFWWVVFWGAAGNLAGSVVSYYLGYFGGRRFLVSYGKFLFITMHDLE
                     MADRLFGKYGSAIALFSRMLPVVRTFISFPAGVAKMNIWKFSLYTLAGSFLWSIFLTY
                     IGVALGENWHSLEGYFRKFDWLIVGILLLLGIWWIWRHIKILKQENHG"
     gene            complement(3020..4066)
                     /locus_tag="VFE94_04325"
     CDS             complement(3020..4066)
                     /locus_tag="VFE94_04325"
                     /inference="COORDINATES: protein motif:HMM:NF027593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HZX50335.1"
                     /translation="MAESKKFPSELRFDPVSKDWVLVATGRARRPETFRTELRNGTRK
                     QWEAAQKACPFDNLDTQERPTYALLRGKEVELPAGNMRVPIQWTTIALPNKYPAFAPQ
                     ESFHTRLVGPYQAADGVGFHEVIVTKDHKKDIPQFSLSQTKELMDMYHARYVALQDAE
                     FVNHISIFKNKGPKAGATVAHPHSQLIATPITDPDIVRSLEGSMRYFEQNQKCIHCVM
                     LAWDLQDKKRIVYANDHFVVVCPFASRVAFEIRLYPSKHASYFENTNDAQRESLAEAL
                     LITMKKLFKGLKDPDYNYFLHTAPADGGNYDHYHWHWEILPKTSTWAGFELGTGIEIS
                     TIEPEKAAEFLRKQ"
     gene            complement(4059..4790)
                     /locus_tag="VFE94_04330"
     CDS             complement(4059..4790)
                     /locus_tag="VFE94_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HZX50336.1"
                     /translation="MAETKLSVIIPAYNEESRLPKTLQAVYAYFQKQNYVYEILVVSD
                     GSKDHTVEVAQEMAQKIPGLRVIAEQENRGKGYAVRKGILEAKGEYRVFTDADNSTSI
                     EHVERMWIEFKKGAEVVIGSRDIKGAKRTVRQSLVREKLGDIFNLMVQMLSGLWGIWD
                     TQCGFKGFSAKAAQEIFAKTTVNRWAFDVEVLVIAKKLGYPIQEIPVVWVNDAESKVR
                     FSGMLKMLVEVLQISIGKVLGKYNG"
     gene            complement(4840..5028)
                     /locus_tag="VFE94_04335"
     CDS             complement(4840..5028)
                     /locus_tag="VFE94_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50337.1"
                     /translation="MIESILAYILGGIIVGVGVFIWLVLGLIQGPDRPHEYLPAFIKA
                     SPILLLPVAILLYLNALD"
     gene            complement(5054..5803)
                     /gene="map"
                     /locus_tag="VFE94_04340"
     CDS             complement(5054..5803)
                     /gene="map"
                     /locus_tag="VFE94_04340"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZX50338.1"
                     /translation="MISLKTPQEIAIMREGGHMLARIVKELVAEVKPGVTTDAIDNLA
                     KQKILKAGAKPAFLNYQGFPRTICTSVNEEVVHAIPSSQKVLKEGDILTIDAGLIWKG
                     YYLDMARTVPVGGVSDDTAHLVRVTKKALRLGVKKMRPGITVGDIGNTIQRFVESQGY
                     GVVRELCGHGIGKELHEEPQIPNYGSRHKGLELKEGMVLCIEPMVTMGDFNVKRAKDG
                     QTFLTRDGSLAAHFEDTIAVTATGAEVLTTV"
     gene            complement(5790..6497)
                     /locus_tag="VFE94_04345"
     CDS             complement(5790..6497)
                     /locus_tag="VFE94_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HZX50339.1"
                     /translation="MTPSNKSLRAVLLFGPPGSGKGTQADLLATRLGFFHVDSSKALE
                     EWFRNVDDKEAIEVDGQKYYALDERKLWEGGILMSPPVVAHIMQEKIRQVHSAAKDIV
                     TSGSLRTLYEATHLYPILEELYGKENIMAFFLKLDEEQSIYRNSHRRICELVRHSILW
                     SEETEKLLHCPLDGSTLVKRENLDDEETIKIRLREYAERTEPVFAYLEKNEISLKEID
                     GNRPVAPVFQDIQHDLA"
     gene            complement(6484..7779)
                     /gene="secY"
                     /locus_tag="VFE94_04350"
     CDS             complement(6484..7779)
                     /gene="secY"
                     /locus_tag="VFE94_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZX50340.1"
                     /translation="MPVILQKVLQIFRLADLRGKILFVLGIFILFRVMANIPIPGIDS
                     SRLQAFFETNQLFGLLNIFTGGALDNLSLAMLGLGPYITAVIVMQLLTMIFPALERMY
                     KEEGEAGRKKFNQYGRILTVPFALVQGYGFLTLLVRQGIIPPLSPVDYVTALVTVIAG
                     TVFLMWLGELITEKGIGNGVSLLIFAGIVAEFPASLRQLFVDFGDWDPAEALDLAAFF
                     AMAVAIIAAVVFVTEARRNIPVSYAKRVRGNKMYGGTSSYLPLNVNPAGVIPIIFALS
                     LLTFPVMVANFFAASPGVTGDIARWIGAYLNPNSWLYILAYFLLVGVFTFFYTAVTFD
                     PKTISTNLQKMGGFVPGVRPGPSTANHISYILNRVLLIGALFLGTIAIMPFLVQRGTG
                     ITAFSFLLGGTALLIMVNVALETMRQIRAQLQMRDYDTF"
     gene            complement(7820..8254)
                     /locus_tag="VFE94_04355"
     CDS             complement(7820..8254)
                     /locus_tag="VFE94_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HZX50341.1"
                     /translation="MQIHQVQPKTKNRKGRRIGRGGKKGTYSGRGMKGQKSRAGTHFQ
                     PRIRELIKKYPKLRGYRAGATPNDIIAVNLDVIEKHFGASEQITPAALVEKRIIAKMK
                     GRVPKVKVLGRGEISKKLSFEGCQVSQSAKEKIEKAGGTVAV"
     gene            complement(8223..8822)
                     /locus_tag="VFE94_04360"
     CDS             complement(8223..8822)
                     /locus_tag="VFE94_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZX50342.1"
                     /translation="MTEIPQTTTRDTRKTRTPGRFDPRRPGQGRSGSPRQGRGDSQRQ
                     ESDSRLLDLARVTRVRAGGRRFSFRAVVVWGDKQGHVGVGVAKGGDVAQAVEKASRHA
                     KKRSITVAIAEGTIPHEVEAKFGASRILLKPQKGGHGLVAGGPVRIICEFAGIQNISG
                     KFLSKTHNKLNNAMVTIEALRKLKAQKQKNADTPSTTEN"
     gene            complement(8827..9177)
                     /gene="rplR"
                     /locus_tag="VFE94_04365"
     CDS             complement(8827..9177)
                     /gene="rplR"
                     /locus_tag="VFE94_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZX50343.1"
                     /translation="MTKPTRKNRKMQRHRRIRAKISGTAERPRLFVFHSNAHLYAQLI
                     DDEKGKTIAAVSDLKLKAKGKGADLAKKLGKEIAEKAKEYKVTQVVFDRGGAKYHGNL
                     KAFAEEVRAQGIAL"
     gene            complement(9181..9729)
                     /gene="rplF"
                     /locus_tag="VFE94_04370"
     CDS             complement(9181..9729)
                     /gene="rplF"
                     /locus_tag="VFE94_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZX50344.1"
                     /translation="MSRIGKKPIEIPQGVEIQVQGQKVNVKGPKGELSREFRPELKIE
                     KQEHEVIITPVNSERETRALWGLTRTLLANMIQGVTQGFEKRLELEGVGYRASAEGDS
                     LALQIGFSHPVKMKAPAQTTVSVEKNVITIGGIDKEQVGQFAARIRKVRPPEPYKGKG
                     IRYEGETIRRKLGKKAAGATAA"
     gene            complement(9737..10126)
                     /gene="rpsH"
                     /locus_tag="VFE94_04375"
     CDS             complement(9737..10126)
                     /gene="rpsH"
                     /locus_tag="VFE94_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZX50345.1"
                     /translation="MVDPIADMLNRIRNAQAVKKENVEVPYSTIKYGIARILESNGFI
                     KGIELKGKRTKKIMELGLQYKDAAGQMGGAKRVSRPGQRQYVSALEIPRVKGGHGIAI
                     LSTSKGLMSNREAKKAHIGGEILCEIW"
     gene            complement(10135..10320)
                     /locus_tag="VFE94_04380"
     CDS             complement(10135..10320)
                     /locus_tag="VFE94_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZX50346.1"
                     /translation="MASKAQISKAKKKPKFSTRQTNRCFRCGRKRGYMRDFDLCRICF
                     RELANRGMIPGIKKSSW"
     gene            complement(10327..10887)
                     /gene="rplE"
                     /locus_tag="VFE94_04385"
     CDS             complement(10327..10887)
                     /gene="rplE"
                     /locus_tag="VFE94_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZX50347.1"
                     /translation="MADMKTVYEKKAVPHMKEKFGYKSRMALPRIEKVVINTGFGRLI
                     GTKTGDDHRKTVEAISGDLSQIAGQHATVTRAKHSIAGFKLREGSPVGAKVTLRGKRM
                     YDFLDRLVHVVLPRSRDFRGIDLTTVDQKGNLSLGIKEHIFFPEISPEKVRDIFGLQI
                     TVATSARNKKEGVELFRVLGFPLKEQ"
     gene            complement(10889..11194)
                     /gene="rplX"
                     /locus_tag="VFE94_04390"
     CDS             complement(10889..11194)
                     /gene="rplX"
                     /locus_tag="VFE94_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZX50348.1"
                     /translation="MRIKKGDNILVIAGKDRGRKGKVVRALPQEAKIVVEGINMRTKH
                     VRAKRAGQKGQMVQMPSPFDVSNVKIVCEKCQKATRVGYKVEGNTKTRICKKCGAPI"
     gene            complement(11199..11567)
                     /gene="rplN"
                     /locus_tag="VFE94_04395"
     CDS             complement(11199..11567)
                     /gene="rplN"
                     /locus_tag="VFE94_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZX50349.1"
                     /translation="MIQPKTKLKVADNTGAKIIQCFHVLGGTRRRYAQIGDIIVAAVK
                     DAEPRRAVKKHEVVRAVVVRQKKAFRREDGSYIRFGENAAVLLEKEKEPKGGRIFGPI
                     PREIREKGFTRIAALAPEVL"
     gene            complement(11570..11803)
                     /gene="rpsQ"
                     /locus_tag="VFE94_04400"
     CDS             complement(11570..11803)
                     /gene="rpsQ"
                     /locus_tag="VFE94_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZX50350.1"
                     /translation="MTKKRLTGIVVSDKMAKTVVVRVERLKEHPKYLRRIRVHKKYKA
                     HDENGEYHVGDKVTIEESRPLSKDKHWIVKGKG"
     gene            complement(11805..11993)
                     /gene="rpmC"
                     /locus_tag="VFE94_04405"
     CDS             complement(11805..11993)
                     /gene="rpmC"
                     /locus_tag="VFE94_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZX50351.1"
                     /translation="MDAKDFQKKTEADLQKILEEKRAKLRQLRFDLPGGKVKNVREIR
                     STRKDIARILTLAKQKQQ"
     gene            complement(11996..12406)
                     /gene="rplP"
                     /locus_tag="VFE94_04410"
     CDS             complement(11996..12406)
                     /gene="rplP"
                     /locus_tag="VFE94_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZX50352.1"
                     /translation="MLTPKKVKHRKWMKGRSRNVGVETRGTEISFGSYGLKSEESKWV
                     TARQLEAGRRAIIRYLRKGGKMWIRVFPDKPITRKGAEVPMGGGKGAVDHYAVSIKPG
                     RVIFEIDGLKDEVAKEALRKAAAKLPVRTKVIKA"
     gene            complement(12408..>12845)
                     /gene="rpsC"
                     /locus_tag="VFE94_04415"
     CDS             complement(12408..>12845)
                     /gene="rpsC"
                     /locus_tag="VFE94_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZX50353.1"
                     /translation="GSGIEELKREIEKIIRKDKQSRYRTPKETGKREMKVEIREIKNP
                     WDSASLTAQWVAQQLEKRMPYRRVLKQALSKVMANRAIEGAKVEISGRLGGADIARRE
                     KLKMGRLPLQTMRAQIDYALTEARTTYGSIGVKVWINKGEKFD"
BASE COUNT         2958 a         3374 c         2803 g         3710 t
ORIGIN      
        1 aacttcttgg cgagtttgcg aacttcgggc ttggtatagt cgccaatggg aaacattacc
       61 ttatctagaa cctcgggttt tataaacgac aaaaaataag attggtcttt gttttgatct
      121 tttgccataa agatagatcc cttatggacc cttgcataat ggccggttgc cacatagtct
      181 accccaagct ccattgcctt gtcaaaaaac aagccaaact taatttcctt gttgcacatg
      241 atgtcggggt ttggcgtaat gccctttttg taaccgtcga gcaaatattg caccactcgc
      301 tgcctgtatt cttttatgag atttatggaa taaaacggaa tgccaatttt ggcagcagcc
      361 aagcgcgcca tacgctcgtc gtcggtggcg gtgcagtttt cgccaaaagg atcttgggca
      421 ctccagcatt tcataaacat tcccgtcaca tcaaaaccag cctttttgag caaggcggca
      481 gaaacagagg agtctactcc ccccgacatc gcaacaatga cgcgtttctt gactttcatg
      541 aacccaatta tgccacaaaa gcaaaaaccg cgcaactcgc cgcttcgctt gtgtttttaa
      601 gcaaattcta gtacagtatg agcaggctta ggaggtagca ccttatgaat tatttcgcag
      661 gggccatagt gtttctagta ggattctcca tagcgattgc cgtactgctt ggttgcatag
      721 ctataaataa gtaccttaaa ggtaagtcct accgcaaaat cgacgttaac gtcttggcct
      781 tgcttgtaac aggaacaatt cctatgggat tgaccgtggc ctcatgggaa gttttccagg
      841 gtttgcgccc atatttcctg tatgccagct tactctgggg cattacgttc gggctgtcgt
      901 catttgtgtg gctctgcatt tccctaatga gtccgatttt ttacttaggg gggcgcttga
      961 aagattcatg gcttgacttc tggttccaac tactcgatcc cgcaggttgg gaaaatcgct
     1021 ttgcatttcc ctatggaaga cgcttgcgca ctttaagaca gtcagcgaag gcaggacatt
     1081 ttgatctgga acaactcccc ttcgttatgt gggttaataa gcaactcgga acagggccag
     1141 gatcaaaggg caagaaataa ataccgcaac acctcggcaa agtcgaggtg tttttattgc
     1201 ttaagagaaa cggtcagcta ctgttgaggc ggcaaaggag tatggcttga tcttcggttc
     1261 aaacccgttg ccgcgaataa gcccggttac ctcttttacc atgtcgcgcg taatgccttt
     1321 ttcgccaata tcagcatgaa tatactgaaa attgtattcc aaaccgttcg ccatagctaa
     1381 aggagtaaag ttttcgcgca gccataaagc cagctggcaa gaaagcatta cctcttccat
     1441 aatgcgctgc ctgcggcttg gaaatgggcg cgactcgtag tgcacgcggc gcaggaaaaa
     1501 tctgccgcct tgctgtttgc gcaacactac aataacgaca ggaaatgtag gatcttctgt
     1561 ggccgaggaa tcgcagccta caataacctc atatttttta tgcggcgttt ctttcatgta
     1621 ggccaccatt tcttccagaa cctcctgtag ataaagagag cctttggtag gattaaaaaa
     1681 cttttcgttt aaaaaatatt ccactaaata cctattctac tgcattgcgg cgtttctggc
     1741 aatagttatg aattctttcg catccaaaga ggcagaccca acgagtacgc cttgcactcc
     1801 tgcctctttg agaaatgcgc cgatgttcaa actgtcgacg cttccgccat acaatatggg
     1861 gctggttttg agcgtttgtc gcatttgcac aactcttgca gctacgcggc cggcattggc
     1921 ggcttttcct ccctcggtac taattgccca ctctggctca tacactacaa taagatttgg
     1981 cgcgtcttga ggcgtttgtt cgttttcccc tatgcacaag ataactttca ggccggctgc
     2041 aagcgctgcc tgcacctttt tttgcaccat atcttcggtt tctccaagat attttgcgcg
     2101 ctctgaatgt ccaagaatta cgtgggaaca cccagcctct ttgagcattt gcggggaaat
     2161 ctcgccggta aaagctcctt tttcttccca aaaacaatcc tgccctccta cgcttaaggc
     2221 tggagaggac gcagcgcgca ctatatcggg aatataaaca aatggcgggc aaatgacgat
     2281 ttctgcgcca tcgatccctt tcacgccttc cgccaccttt tcaaaaagct cctgcgcttc
     2341 tctcgcggtt gcaggattca ttttccagtt tgctatgata ataggtttat ccatggtttt
     2401 cttgctttaa aatctttata tgccgccata tccaccatat ccctaatagt aacaaaatgc
     2461 caacgattaa ccagtcgaat tttcgaaaat atccttccag cgaatgccaa ttttccccga
     2521 gtgccactcc aatgtaggta agaaaaatac tccataaaaa agaccccgca agcgtgtaca
     2581 gagaaaactt ccaaatgttc attttcgcca cgcccgcagg aaaagaaatg aacgtacgca
     2641 ccacaggcag catgcgtgag aacaacgcaa tggcagagcc gtatttgcca aacaggcggt
     2701 ctgccatttc aaggtcgtgc atggtaataa agaggaattt cccgtagctg accaaaaaac
     2761 ggcgtccccc gaaataccca aggtaataag aaaccacgga ccccgcaagg tttcccgcag
     2821 ctccccaaaa caccacccac cagaaagaaa actcgccttt ggacaccaaa tatccggcaa
     2881 acggcatgat aatttccgaa ggcacaggga tgttggcgct ctctgctgcc ataagcagcg
     2941 caatgcccgc gtaccctgtt gcttctatgc tgtacatgac catggaggcc aaaagttcaa
     3001 gaaggtgttc cataatgctt tattgtttgc gcaaaaactc tgctgctttt tccggctcaa
     3061 ttgttgaaat ctcgatgccc gttcccagtt caaacccggc ccaggtggaa gtttttggga
     3121 gaatctccca atgccagtgg taatggtcgt agtttccccc gtcggcggga gccgtatgca
     3181 aaaaatagtt gtagtcggga tcctttaacc ccttaaaaag ttttttcatt gttatgagta
     3241 aggcttcggc cagcgattcg cgctgcgcat cgttcgtatt ttcaaagtat gaggcgtgtt
     3301 tggagggata caagcgtatt tcgaaagcca cgcgcgaagc aaacgggcat accaccacaa
     3361 agtggtcgtt tgcgtataca atgcgctttt tgtcttgtaa gtcccatgca agcattacgc
     3421 agtgaatgca cttttggttt tgttcaaagt accgcattga gccctcgagg ctgcgcacaa
     3481 tatctggatc ggtaatggga gtggcaataa gctgcgagtg cgggtgggcc accgttgctc
     3541 ctgcctttgg ccctttgttt ttgaagattg agatatgatt tacaaactcc gcgtcctgga
     3601 gtgccacata cctggcatga tacatatcca ttagttcttt tgtctgcgag agtgaaaatt
     3661 ggggaatatc ctttttgtga tctttcgtga caataacttc gtgaaaccca acgccgtcgg
     3721 ctgcctggta aggccctact aatcgcgtat ggaaggactc ttgaggagca aatgccgggt
     3781 atttattggg aagagctatt gttgtccatt ggataggaac acgcatgttg cccgcgggca
     3841 gttctacttc cttgccgcgc aacaaggcat acgtagggcg ctcctgtgta tctaaattgt
     3901 cgaagggaca agctttctgc gccgcctccc attgctttct tgttccgttg cgaagctcgg
     3961 tgcgaaaagt ttccggcctg cgggcgcgac ccgtggcaac cagcacccag tccttcgaaa
     4021 ccggatcgaa tcgcaactct gatgggaatt tttttgattc agccattata tttacccaaa
     4081 accttgccga tgctaatctg taaaacctcc acgagcattt ttagcatgcc agaaaacctt
     4141 actttgcttt ctgcgtcgtt tacccatacc accgggatct cttgtatagg atatcccagt
     4201 tttttggcga ttaccagcac ttccacgtcg aacgcccaac ggtttaccgt agttttggcg
     4261 aaaatttcct gggcagcttt tgcagaaaac cccttgaacc cgcattgcgt atcccaaatc
     4321 ccccacaaac cagataacat ctgcaccata aggttaaaaa tgtcacccag cttttctctt
     4381 accaaacttt gccgaacggt acgtttagct cctttaatgt cgcgagaacc tataaccacc
     4441 tccgctccct ttttgaattc gatccacatc cgttctacgt gctcaattga ggtagaattg
     4501 tccgcatccg tgaatacccg gtattccccc tttgcctcaa gtatgccttt gcgtacggca
     4561 tatcctttac cgcgattctc ctgctctgca ataacgcgta gaccaggaat tttctgtgcc
     4621 atttcctgcg ccacttctac cgtatggtct tttgaaccgt cggaaaccac aaggatctcg
     4681 tatacgtaat tctgcttttg aaagtacgca taaaccgcct gtagcgtttt tggcagccgg
     4741 ctttcctcgt tgtatgccgg gattataacc gaaagcttcg tttcagccat gttgccatct
     4801 taccacaaaa caacagctgg aaaaaataga aagccgcgcc taatccaggg cgttgagata
     4861 gagcaaaatt gccacaggaa gcagcaaaat ggggctggct ttgataaagg caggcaggta
     4921 ttcgtggggc ctgtctggac cttgaattaa acccagaacg agccagataa aaacgccaac
     4981 acccacaata atccctccca aaatatatgc gagaatggat tcaatcattt cttttactta
     5041 ccacaaatct gtcttatacg gtagtcagca cttccgcccc ggtggcggtg acagcaatag
     5101 tatcttcaaa atgggcagcc aaagagccgt cgcgggtaag aaaggtttgg ccgtctttgg
     5161 cgcgttttac gttaaagtct cccatagtga ccatgggctc aatgcatagt accatgcctt
     5221 ccttgagctc taaccctttg tggcggcttc catagtttgg aatttggggc tcttcgtgca
     5281 gttctttgcc aatgccgtgt ccgcaaagtt cgcgcaccac cccgtatccc tggctctcca
     5341 caaaccgttg aatggtattc ccaatatcgc ccaccgtaat tccggggcgc atctttttaa
     5401 ctcctagcct tagcgctttt ttggtaacgc gtaccaggtg cgctgtgtcg tcggaaactc
     5461 cgcccacggg aacagtgcgc gccatatcaa gatagtatcc tttccatatc aaccccgcgt
     5521 ctatggtaag aatatctcct tccttaagca ctttttggct cgaaggaatc gcatgtacca
     5581 cctcttcatt taccgatgtg caaatcgtgc gcggaaatcc ctggtagtta agaaaagcag
     5641 gcttggcacc tgcttttaaa attttttgtt ttgcaaggtt atcaattgcg tcggttgtca
     5701 ccccgggctt cacctcggct acgagctctt tcacgatgcg ggccagcata tgccctcctt
     5761 cccgcatgat tgcgatttct tgaggcgttt taagcgagat catgttggat atcctgaaaa
     5821 accggggcga ccggacgatt gccgtcgatt tcttttaggg aaatttcgtt tttctccaag
     5881 tatgcaaata ccggttcggt tctttcggca tactctcgaa gccgtatctt gattgtttct
     5941 tcgtcgtcga ggttctcacg ctttaccagt gtggagccgt cgaggggaca atgcaaaagt
     6001 ttttcggttt cttcgctcca aagaattgaa tgccttacta actcgcagat gcggcgatgt
     6061 gaattccggt atatagactg ttcttcatcg agctttaaga aaaacgccat aatgttttcc
     6121 tttccgtata attcttccaa gatgggatac aaatgcgtag cttcgtacaa cgttcgcaga
     6181 gaaccgctgg taacaatgtc ttttgcggca gaatgcacct gccggatttt ttcttgcatg
     6241 atatgagcaa caaccggagg gctcataaga atgccccctt cccagagttt gcgctcgtcg
     6301 agcgcatagt atttttgccc gtcaacctct atagcttctt tgtcgtcaac attgcggaac
     6361 cattcctcaa gcgccttgct cgaatctaca tggaaaaatc caagtcttgt agccaatagg
     6421 tcagcttgag tgccttttcc agaccccggc ggcccaaaca gaagtactgc acgcaaactt
     6481 ttgttagaag gtgtcatagt cgcgcatttg gagttgggcg cgtatttggc gcatagtttc
     6541 caacgccacg tttaccataa taagcagtgc cgtgccgcca agcaggaaag agaaagcggt
     6601 aatgcccgta ccgcgctgca ccaaaaacgg cataatagca attgtgccca aaaacaaagc
     6661 gccaatgagc aatacgcgat ttagaatata cgaaatatgg ttcgcagtgg agggcccggg
     6721 gcgcacgccc ggcacaaagc ctcccatttt ttgcaaattg gtggaaattg ttttggggtc
     6781 aaaggtaact gcggtataaa aaaaggtaaa tacgcccacc aaaaggaagt aggcaagaat
     6841 gtagagccaa gaattggggt tcaaatacgc gcctatccaa cgggcaatat cgcccgtaac
     6901 tcccggggaa gccgcaaaaa aattagccac cattacgggg aacgtaagaa gagataaggc
     6961 aaaaattatg ggaattacgc ctgccgggtt tacgttaagg ggcaggtagc tcgaagtgcc
     7021 tccgtacatt ttgtttccgc gcactctttt ggcgtaagaa accggaatat tccgccttgc
     7081 ctctgttacg aatacgacgg cggcaataat ggcaactgcc atggcgaaaa aggccgcaag
     7141 gtcaagcgct tctgcaggat cccaatcgcc gaaatccaca aaaagctggc gcaaacttgc
     7201 cgggaattca gccacgatac cggcgaaaat aagcagcgaa acgccgttgc caattccctt
     7261 ttccgtaatt aattcaccca gccacattaa gaatacggtt cccgctatta cggtaaccag
     7321 cgcggtaaca taatcaacag gagataaagg cggaattatt ccctggcgca ccaaaagcgt
     7381 aagaaaacca tagccttgta caagagcaaa tggtactgtt aaaatccttc cgtactggtt
     7441 aaactttttg cggccggcct caccttcttc tttgtacatt cgctccaatg cggggaaaat
     7501 catggtaagc agctgcatga cgattactgc ggtaatatac ggccccaggc ccaacatggc
     7561 cagagataag ttatcgagcg cgccaccggt aaaaatgttc aaaagcccga aaagctggtt
     7621 ggtttcgaaa aatgcctgca aacgggagga gtcaataccg ggtatgggaa tattcgccat
     7681 tactcggaaa agtataaaaa tgccgagcac aaataaaatt ttgccccgga gatcggcgag
     7741 ccgaaaaatc tggagtacct tttgaagaat tactggcata ctattctact agttataaca
     7801 gttataaaca ttatagaggt tataccgcta ccgttcctcc tgctttttcg attttttctt
     7861 ttgcgctttg ggaaacctgg cacccctcaa atgagagctt tttggaaatc tcccccctcc
     7921 ccaacacttt caccttaggc accctccctt tcattttagc aataatgcgc ttttctacca
     7981 acgctgcagg ggtaatctgc tcactggcgc caaaatgttt ttctatcaca tccaaattca
     8041 ctgcaataat atcgtttgga gttgccccag cacgataccc ccgtagcttg gggtatttct
     8101 taataagctc gcggatgcgc ggctgaaagt gggtgccggc ccttgatttc tggcctttca
     8161 tgccgcggcc agaatacgtg ccttttttgc cgcctcttcc aatgcgtctt ccctttcgat
     8221 ttttagtttt cggttgtact tggtgtatct gcattttttt gtttttgcgc tttcaatttt
     8281 ctcaaagctt caatggtaac cattgcattg ttcaacttgt tgtgggtttt ggacaggaac
     8341 tttcccgata tgttctggat ccccgcaaac tcgcaaataa tgcgcaccgg tcctcctgcg
     8401 acaagaccgt gtccgccttt ctggggcttt aacaaaatgc gcgatgcccc gaatttggct
     8461 tccacttcgt gagggattgt accttccgcg atagccaccg taatgctcct tttctttgcg
     8521 tgcctgcttg ccttttccac tgcctgggca acgtcgccgc cttttgcaac gcctacgccc
     8581 acatgcccct gtttatctcc ccatacaact actgcacgaa aactgaatct ccggccaccg
     8641 gctcgaaccc ttgttaccct cgccaaatca agcaggcggg aatcagactc ttgcctttgt
     8701 gaatctcccc ttccctggcg tggagatcca gagcgtcctt gccctggcct tctcggatca
     8761 aaacgccccg gtgttctggt ttttcttgtg tctctggttg tagtttgtgg tatttctgtc
     8821 atatggttac agcgcaatgc cctgggctcg cacttcctcg gcaaatgctt tcagattgcc
     8881 gtggtacttg gcgcctccgc ggtcgaatac gacttgcgtt actttatatt cctttgcctt
     8941 ctctgcaatt tctttcccta attttttagc aaggtctgcg ccttttccct ttgccttgag
     9001 tttcagatcc gaaaccgcag ctatcgtttt ccctttctcg tcgtcgataa gttgcgcata
     9061 caagtgtgcg tttgaatgga acacgaaaag gcggggacgc tctgcggttc ctgaaatttt
     9121 cgcgcgtatc ctgcggtgcc tttgcatttt tcggtttttt cgcgtgggtt tcgtcatagg
     9181 ttaagcggct gtcgctcctg cagctttctt gccaagtttt cttcttatag tttctccttc
     9241 atagcggatc cctttccctt tataaggctc gggggggcgc acctttcgaa tgcgggcggc
     9301 aaactgccca acctgctctt tgtctattcc gcctatggta atcacgttct tctctacaga
     9361 aacggttgtt tgggcgggcg ccttcatttt taccggatgg gaaaaaccaa tctgcagcgc
     9421 aagggaatct ccttccgctg acgcgcgata ccccactcct tcgagttcca ggcgtttttc
     9481 gaaaccctgc gtaaccccct ggatcatatt ggccaaaaga gtacgcgtga gcccccaaag
     9541 cgctcttgtt tcgcgttcgc tgttgaccgg agttataata acctcatgtt cctgtttttc
     9601 aattttcagt tccggccgaa actcacggga gagttctccc ttggggcctt ttacgtttac
     9661 tttctggccc tgcacctgga tttcgacgcc ttgcggtatt tctatgggtt tctttcctat
     9721 acgtgacata agtttgttac caaatttcgc aaagaatttc tccaccaata tgcgcttttt
     9781 tggcttccct attgctcatt aaacctttcg aggtggataa aatggcgatt ccatgcccac
     9841 cttttactct cggaatctct aaggccgata catactggcg ttggccgggg cgagaaacgc
     9901 gtttcgctcc tcccatctgg cccgctgcgt ctttatattg caagcccaac tccataatct
     9961 tttttgttct tttgcctttc aactctatgc cctttataaa gccgttgctt tccaaaatcc
    10021 tggcgatacc gtattttatt gtagaataag gaacttccac gttctctttt ttaacggctt
    10081 gggcgtttcg aattctatta agcatgtctg ctattggatc taccatagta tgcgttacca
    10141 actactcttt ttaatcccgg ggatcatgcc tcggtttgcc agttctcgga aacaaatgcg
    10201 gcacaaatcg aaatctcgca tgtacccgcg cttccgtccg caccgaaagc agcgattggt
    10261 ttggcgcgta gaaaacttcg gcttcttttt ggctttggaa atttgggctt tgcttgccat
    10321 atacaattat tgctctttca atggaaatcc taatacgcga aataactcga ctccttcttt
    10381 tttatttctt gcgcttgtcg ctacggtaat ctgcaaaccg aaaatatcgc gtactttttc
    10441 gggggaaatt tcagggaaaa agatatgttc tttaatgccc agcgaaagat ttcccttttg
    10501 atccaccgtc gtgaggtcga tgcctcggaa atcccgggaa cgcggcaaca ccacatgcac
    10561 caaacgatcc agaaaatcat acatgcgctt gccccgcagg gttacttttg cccccaccgg
    10621 cgagccttcc cgaagcttga atcccgcgat ggaatgtttt gcacgggtta ctgttgcatg
    10681 ctgtcccgcg atctgactca agtctccgct gattgcctct acggtctttc ggtggtcgtc
    10741 tccggttttt gtcccgatta aacggccaaa cccggtgttg attaccacct tttctattcg
    10801 gggcaatgcc atacggcttt tatacccgaa cttttctttc atatgaggca ccgccttttt
    10861 ttcgtatact gtcttcatgt ctgccatatt atattggggc tccacatttt ttgcatatac
    10921 gggtctttgt attcccctca accttatatc ccacgcgtgt tgctttttgg catttttcgc
    10981 aaacaatctt tacattcgat acatcaaaag gactcggcat ttgcaccatt tggccttttt
    11041 gcccggctcg cttggcgcgc acgtgtttgg tgcgcatgtt gattccttca accacaatct
    11101 tggcttcttg gggaagcgct cttaccactt ttcctttccg tccgcggtct ttccctgcaa
    11161 tcactaatat gttgtcgcct ttcttgattc gcatagtgtt ataaaacttc gggggctaac
    11221 gcggcaattc tggtaaatcc cttttcgcgg atttccctgg gtatgggccc gaaaatgcgc
    11281 ccgcccttgg gttctttttc tttttccagc aagaccgccg cgttttctcc aaatcgaata
    11341 taggaaccgt cctcgcgcct aaacgctttt ttctggcgaa caaccactgc cctgaccacc
    11401 tcgtgtttct ttaccgctct tcggggttcg gcatctttta ctgcggcaac aataatatcg
    11461 ccaatctggg catacctccg gcgcgttccg ccaagcacgt gaaaacactg gattatcttg
    11521 gctccggtat tgtcggcaac ttttaatttg gttttgggtt gaatcatatt tagcctttgc
    11581 cttttacaat ccaatgcttg tctttgctca agggtcggga ttcctcgatc gtcactttgt
    11641 cgcctacatg gtactcgccg ttttcatcgt gcgctttata tttcttgtgc acgcgaatac
    11701 gccgaagata tttcggatgc tctttcaacc gttccacccg caccactacg gtttttgcca
    11761 tcttgtctga taccacaatg cctgttaagc gtttttttgt catactattg ctgtttttgc
    11821 tttgccaatg ttaatattcg ggcgatgtct tttctggtgc tgcgaatttc ccgcacattt
    11881 tttactttcc cgccgggaag atcaaaacgc agctggcgaa gttttgccct tttctcttcc
    11941 aatatttttt gcaaatccgc ctcggttttc ttttgaaaat cttttgcgtc catgcttagg
    12001 ctttaataac ttttgtcctc acgggcagct tcgcggcagc ttttcgcagc gcctcttttg
    12061 ccacttcatc tttcaggccg tctatttcaa aaatcactcg ccccggctta atacttacgg
    12121 cgtaatggtc aacggctcct ttcccgccgc ccatgggcac ctcggctcct ttcctggtta
    12181 tgggtttatc gggaaatacg cgaatccaca tttttccgcc ctttctcaaa taacggatta
    12241 tggcgcgccg tcccgcctcc agctggcggg ctgttaccca tttgctttct tcggatttca
    12301 acccatagct tccaaaggaa atttccgttc cccgggtttc aacacccaca tttcgggaac
    12361 gccctttcat ccattttcta tgttttactt tctttggtgt caacatatta gtcgaacttc
    12421 tcccccttgt taatccacac ctttacccca atgcttccat aggtggttcg agcttccgtg
    12481 agcgcataat caatttgcgc gcgcatggtt tgcaggggca gccttcccat tttgagcttc
    12541 tcccttcttg caatatctgc tccgcccagg cggcccgata tctccacctt tgcgccttca
    12601 attgcgcggt ttgccattac tttagaaagc gcctgcttaa gcactctccg atagggcatt
    12661 cgcttctcta actgctgggc aacccactgg gcggttaaag aggccgaatc ccagggattc
    12721 ttgatttccc taatctccac cttcatctcg cgctttccgg tttctttggg tgtccggtat
    12781 ctgctttgtt tgtctttccg gataattttt tcgatttccc ttttcaattc ttcaatgcca
    12841 gaacc
//
LOCUS       DASRUP010000108         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_1876977, whole genome shotgun sequence.
ACCESSION   DASRUP010000108 DASRUP010000000
VERSION     DASRUP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876977"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFE94_04420"
     CDS             complement(<1..649)
                     /locus_tag="VFE94_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HZX50354.1"
                     /translation="MAKSQHYKKVYQKQGPTRTWTARLLQVAFFGILLMPVAAFATFA
                     YYAKDLPRPEKFSEVTLAQPTKIYDRTGEVLLYEVFGEEKREVVPLTEIPLYFQQAVV
                     ATEDAEFYEHSGVSVKGTGRAVLINLGLRDSVFSRPGGSTITQQLARNSFLSSEKTIP
                     RKIREFILTLELERQYSKNEILGFYLNQIPFGSNAYGIGAAAELYFAKKPADLTLP"
     gene            744..1895
                     /gene="tyrS"
                     /locus_tag="VFE94_04425"
     CDS             744..1895
                     /gene="tyrS"
                     /locus_tag="VFE94_04425"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZX50355.1"
                     /translation="MQKPTVTIENLLGRQVEQILPGRKELEELMKKRKIRLYLGVDPT
                     GRNLHLGHTIVLRKLQQFAELGHEAILVVGTGTVLAGDPSQRDAARARITKKEIAENI
                     KTWKKQVSRVVDFSKVTIKENGDWLAKLRLPEILDIASRISASQLFQRDMFQARLRRG
                     DTVWTHELLYPLLQGYDSVAMDVDLEIGGTDQLFNMLVGRELQKKMRNKEKYVLTTPM
                     LLGVDGKPMSKTSGNTVNILDSPQEMYGKLMGMKDELTSHYAELCCDIPFGELNKLSP
                     RDAKARVAKELVRIYHGQEQAQEAEEEFERVFREKQAPSSVHKVSIQEEMPLVEFLVL
                     ADLASSKAKARRLIEQRGVRVNGEVRQNPAEPIRRGMRLQVGKRRFVDT"
BASE COUNT          530 a          472 c          592 g          503 t
ORIGIN      
        1 ctggcaacgt gagatccgcg ggttttttgg cgaagtacag ttcggcggcg gcccctatgc
       61 cgtaggcgtt ggaaccaaac ggaatctgat taaggtaaaa gccaaggatt tcgtttttgg
      121 agtactggcg ttcgagttct aaggtaagaa taaactccct gattttcctt ggaatagttt
      181 tttctgaaga aaggaaagaa ttgcgcgcaa gctgttgagt aatggtggaa cctcccggcc
      241 ggctaaagac cgagtcgcgg agccccaggt tgataagaac cgcccttccc gttcctttca
      301 cgctaacgcc agagtgctcg tagaactccg cgtcttcggt agcaactacg gcttgttgaa
      361 agtagagagg gatttctgtg agtgggacaa cctctcgttt ttcttcacca aagacttcgt
      421 acagcaacac ttctccggtg cggtcgtaaa tcttggtagg ttgggcgagc gtaacctcac
      481 tgaacttttc tgggcgagga aggtctttgg cgtagtaagc aaaagttgca aaagcggcta
      541 cgggcatgag aagaatgcca aaaaacgcca cctggaggag gcgtgcggtc catgtccgcg
      601 ttgggccctg tttttggtat acttttttgt aatgctgcga ctttgccata aggcagattc
      661 ggggagttgc tggtcataag cataccacat ttttgccggg ttttcaagtt tgctgtttca
      721 agaaagggtg atagaataac ggaatgcaaa agcccaccgt gacaatagaa aacctgctgg
      781 ggcgccaggt ggaacagatt cttcccgggc gcaaagagtt ggaggagttg atgaaaaaga
      841 ggaaaatccg tttgtacctg ggtgtggatc ccacggggag gaaccttcat ttggggcata
      901 ccattgtcct ccgaaagctt cagcagtttg ccgaacttgg gcacgaggca attttggtag
      961 tgggtacagg gacagtgctg gcgggagacc cttcccaaag ggacgccgca agagcccgca
     1021 ttacaaaaaa agagattgct gaaaacatca aaacgtggaa gaagcaagtt tcccgggttg
     1081 tggacttttc aaaggttacg ataaaggaaa acggggattg gctggcaaaa ctccggcttc
     1141 ccgagattct ggacattgct tctcgcataa gcgcgtcgca gctgttccag cgcgacatgt
     1201 ttcaggccag gctccgccgg ggcgacacgg tgtggacgca cgagctttta tatccgctat
     1261 tgcaggggta tgattcagtc gcaatggatg tggaccttga aattggcgga accgatcaac
     1321 tttttaacat gctcgtgggg cgcgaactgc aaaagaaaat gcgcaacaaa gaaaaatacg
     1381 tgttaacaac ccccatgctt ttgggggtcg acggcaagcc catgagtaaa acctcgggca
     1441 ataccgttaa catcttggac tctccccagg aaatgtacgg aaagctcatg ggcatgaaag
     1501 acgagcttac ctcgcattat gcagagctct gctgcgatat tccttttggg gaactgaaca
     1561 aactgtctcc tcgagacgca aaggccaggg tggcaaagga gttggtgcgt atctatcacg
     1621 gccaagagca ggcccaggaa gcagaagaag agtttgaaag ggtgttccgg gaaaaacaag
     1681 caccttcctc tgtgcacaaa gtaagtattc aagaagaaat gccccttgtt gaatttttgg
     1741 ttctggctga tttggcaagt tccaaggcca aagcgaggcg tttgatagag caaagagggg
     1801 ttcgggttaa tggggaggtg cgccagaacc ctgcagagcc aatacgccgc ggcatgcggc
     1861 tccaggtggg aaagcgcaga tttgtagata cataaaaaaa caccctgcag cattagctga
     1921 agggtgtttt tatgggatta tttatgcacc ttcttcggtg tctttcccgc cctcttcgtc
     1981 aagaagattc tggggttctc ccacgggcat tccttctcct ccttccgtgg ttccctcgtc
     2041 aggggtgctt gtcggctctt cgtcgagcat gagaatggtt tctttcataa gcgattc
//
LOCUS       DASRUP010000109        10895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_762922, whole genome shotgun sequence.
ACCESSION   DASRUP010000109 DASRUP010000000
VERSION     DASRUP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762922"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1017
                     /locus_tag="VFE94_04430"
     CDS             226..1017
                     /locus_tag="VFE94_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50356.1"
                     /translation="MGNPEHGPSFFPEAEAERSQEQREFADRLEADPNLFIVFRDSLD
                     LVLVSEGEKPASHIDFWKIPEESEEEFQRKVNQLTALLEARGLSFHTERKTVEGRDGN
                     PVELVGFSVGRSEEELQKFTDADQIEDYDERMVAYGRALGYPETAVEAKPDELIKWYE
                     LPDNVVFNEEVHSSPFGMSREHWQEEIETAREWASVAKEAAPRLYEQATNQILRDRLL
                     YALSETPEELRRIFRSQEARAALEQRNKPLYDNFMRGARANGIID"
     gene            1087..2058
                     /locus_tag="VFE94_04435"
     CDS             1087..2058
                     /locus_tag="VFE94_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZX50357.1"
                     /translation="MFENVINYVDLLVIGLGILYILSAVQAGVFSVLGRLVAFFGALA
                     IAFWGYSYAQDLFVEQFAMPSSFARAIGFLSLFFFTQFLLNLIFGLVFSFIPEKMRYA
                     WWSKVLALAPAVVDAAVLAGVILIFIVLLPFAPKAAQDITESRSGAFALETMSRLEGY
                     ANQVFGEALQDTITFLTVQPETAERVEIPYKPAALHVDEGAEEQMLRLVNEERARAGV
                     SALVTDEEIVQVARAHSKDMWEKGYFSHINLEGKDPFDRMRDEDIEFSAAGENIALAP
                     TTALAHRGLMNSPGHRRNILDPKFQRVGIGVIDGGVYGKMFTQNFAL"
     gene            2062..3579
                     /locus_tag="VFE94_04440"
     CDS             2062..3579
                     /locus_tag="VFE94_04440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZX50358.1"
                     /translation="MFGIPYPKKILFALVLVFIFCSSFAALWSLVGSHTLGWDEAVYL
                     TKARSWAEGTPADEFRPYRPIAMPAFGWIFLHFGDTEEILRLFGALFGAVTLVFLFLL
                     FRSITNSWVALSIVYSVGTSSLFLQQAPQFLNDIPSSGLLFGILWLIWKYYKTEGENK
                     SILLAAPLAALAFYLRYGVVLSLGFIGIVSLLLLWRKFAGKEGTNFSVLAKTFAVFAA
                     FMLPHGIHSFIVEGSALGILRSGSTAGGREYLGEGLLNYIQWLPSALAGPLVGASTIL
                     GIAVTLAIIFRRNLRQNYEGLLWLGSIGMLSFFFTGLLVHAEARYVFFPVALLSGVGI
                     AGVYFLLKKVRLQVVGIGLFALATLLFGVFNYQEAATIVKEKETDISRNEYRETYRAI
                     LQNKPKEEKCVVWSIDYLPQISWYTQCNTLRIAETKNIVVFPNDPLVTPKGAHYSIVF
                     TKSEDGQIQPDNAERYGAFLTQIHRSREFPLFIGRGQATVYRMQPKKSPVRQITP"
     gene            3705..4745
                     /locus_tag="VFE94_04445"
     CDS             3705..4745
                     /locus_tag="VFE94_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50359.1"
                     /translation="MKKIKKHVKNHQGLIVLSGIGIVGLAGAFAFMASAATDCMFTDQ
                     GNTRMLNADCATDATIFIPDGFTLDGRHHTITAVDPTGDHFKGAVVQNAGANAHVKNL
                     VVTTSNLSNACDAGGDRLRGIMFQGASGSIVHNTVYNLNQGASGCQEGNAIEVRNEPF
                     DGTHPDTQTVEIAHNGLTSWQKTGIVANGDVSVDIHHNYVGSSATQPNDLAANSIQLG
                     FGAIGQVVQNRLDGNQWCGSSEFVATAILLFQTSNGAVVKQNIIGGNSDVGIYGFGDD
                     LVVDNNKLTDNTVEDCNQFAYDIGIGNYGSAASLSSFTNNKVNGFDTPYDGVTSGKNK
                     VIPGPQKGQTFF"
     gene            complement(4792..6102)
                     /locus_tag="VFE94_04450"
     CDS             complement(4792..6102)
                     /locus_tag="VFE94_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZX50360.1"
                     /translation="MNTETAAKPRKKIVLIIGLPDYESAFQLTEGWKKRGKKYRYAFL
                     YHQEPSDQEKEAAALFDIAITCDINSDESVEQALWPYKEELAAITVPRFERFIPWFQR
                     LIPFVPHLRTPTVESLSWATSKIEMRKHFAAYNPRITPKFFVAKDATKSTLRAIEQQV
                     GFPVIIKPSGLAASVLVSIAFHTEELEQSLKRIFRKIRAQYRRSGGRGEPQVLVEQFM
                     EGDMYSIDVYVNTSGKIYFCPLVHVTTGRQIGFDDFFGYQRITPTDLVKENVYKARDV
                     SADAIRALTLFNTSVHLELMKTEKGWKIIELGPRVGGFRDFMYRLSYGMHHYANDIAV
                     RAGERPKLSRKTYGHTAVFEFFAKQEGIITNLTGVKKVQELGSFHSMDVNSKIGDRAV
                     FAKNGGKSIFNIFLFSKDRSELLADIRRMEQLIAIETESKPSKK"
     gene            complement(6099..7409)
                     /locus_tag="VFE94_04455"
     CDS             complement(6099..7409)
                     /locus_tag="VFE94_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZX50361.1"
                     /translation="MTKKPRKARKNVILIIGLPNDLHVVKRILAFGKHRKQKYRFAVM
                     MPQNKTLLDEEKEVLHLFDIVLTCNFDSPESIMECLAPYQDGFVAVTARGEQNIPHLK
                     KVIPYLPYLRTPTQDSLEWATSKIEMRRRFTAYDKKITPSYMVVSDTSKKTIKAIVEK
                     IGTPLVIKPSGLAVSLLVTIAFHQEELEKSLRKVFRHLRVQYKKNGGRGEPQVLVEQF
                     MEGDMYSIDIYVDSRGKMYFCPLVHIKTGRTIGFDDFFGYQQMTPTLLAKESIAEAQE
                     ASKKAIHALGLRSTTVHLELMKTEQGWKIIELGPRIGGFRDMLYELAYGIKHTENDIA
                     VRIPERPKLPRKLKGYAAALKIFARQEGVITNFLGVKKVQALESLHSMETNKKIGDRA
                     VFAKNGGKSVFDILLFNKDRSRLLADIRRIEQALKIETKASSRS"
     gene            7480..7941
                     /locus_tag="VFE94_04460"
     CDS             7480..7941
                     /locus_tag="VFE94_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZX50362.1"
                     /translation="MIRAIFLSILFLLVGIGIGAYLFSGTQSRSVLRLQECRQDCLSS
                     QETLGLLASVGIQKIGSALPFIVKETDKTLAIRHPAPRADVHYVIIPKQDIKNPHDVT
                     QEDAPYLADAFVVMRELIEEQGLEDYKIVANGPGFQTVQYLHFHLVSDLPE"
     gene            complement(7977..9104)
                     /locus_tag="VFE94_04465"
     CDS             complement(7977..9104)
                     /locus_tag="VFE94_04465"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX50363.1"
                     /translation="MDNPQDTEETLAQEQGQKPDPLKDPLRSKSLLFGAAAIALVFAV
                     ALVLFQLQGTAPKESAQEPITIGVLRYLPVHDAIVDGLKQGLAEAGYEEGQDVVYTVS
                     EVGFTEEKTRALAKELIAQDVDLIFALASVSAKVAVEETAVANRTDIPIVMSHALNTI
                     NLGIIDSYQSSGNNLTGIEVNFQELTGKKLEFLKRLAPQAKKIGVFDVIHEPGTALNL
                     IKAQLAIEAPRLGLEIVWYPIETEEAGPQLTQELQQALAAIAPGDIDAYLHNGATLLG
                     HEDDKQLIIDRINELKIPSVWVTPVEVRSGGLMTYQYDLLEMGRQSAVQVEKVLSGIQ
                     PAGIPIEFAQTQKLFMNFSTVEELGITVPEDVLELVDVSVE"
     gene            9244..9720
                     /locus_tag="VFE94_04470"
     CDS             9244..9720
                     /locus_tag="VFE94_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX50364.1"
                     /translation="MTTTDTEVRVRGIGHVGILVDDLHRSKKFYEEVVGLEEHNRVPK
                     GSSDQDVQMIFFGTKGGTRTNGVHHALVIRQAPEDVDVSSAVPLTKKAIQQIAFQMGS
                     RQDFEDALAHLAANGVPLTERTRHDETEFDSGSDSAYFQDPDGNRVEFFLMPSSET"
     gene            9908..10282
                     /locus_tag="VFE94_04475"
     CDS             9908..10282
                     /locus_tag="VFE94_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50365.1"
                     /translation="MATPKEIIRDIYYPRLFDGGELWVADEIIAPEFFNNRHPTWPRG
                     PEGIKKVVRSLHKKYDGAFHALHTEILLQVAEGNVVSTLTRQTGKGDEDIHLAELQSH
                     FHIFNDAQLIVEHRVVYMPLPQ"
     gene            10393..>10895
                     /locus_tag="VFE94_04480"
     CDS             10393..>10895
                     /locus_tag="VFE94_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HZX50366.1"
                     /translation="MPQHSTLLRSAIIIILIAGLLLVVAATVGIGAIFMRSDSPSPGS
                     QEMLRIGVVQYAPVSNILTQGFKQGMEELGYVEGVHVEYVETAPTLDNALLSQNARDI
                     ASQNVDLIFATTAVGGLAALNATADIPIVWTHAATAVTNGLAANYNSSGNNSTGIEIS
                     FAELTRK"
BASE COUNT         2742 a         2648 c         2636 g         2869 t
ORIGIN      
        1 cccgcaagct ttccccgggc ttccgcggaa atgctcaaag aatttcaaga cgcctaccct
       61 tcgcttttta aaagtgggga cgcctggtcc atagacaaac atcgcaaacg ctttatggac
      121 tgggttttct cccgtaagaa caacgcataa gcaaaactcc ttccggacgg ttttcggcaa
      181 aaatctcttg tggtaaccgc gagagttttt tggtatatag aaagaatggg aaatcctgaa
      241 catgggccat cgttttttcc cgaggcagaa gccgaacgga gccaagagca acgggaattt
      301 gccgaccgcc tggaggcaga cccaaacctt tttatcgttt ttcgagattc gctcgatctt
      361 gtgttagtaa gcgaggggga gaaacctgcg tcgcatatag acttttggaa aatcccggaa
      421 gaaagcgaag aagagttcca aagaaaagta aaccaactta ctgctttatt ggaagctcga
      481 ggattgtcct tccacacaga acgaaaaaca gtagaagggc gcgacggcaa tcctgttgag
      541 ctggtaggct ttagtgttgg gcgaagcgag gaagagctgc aaaaatttac tgatgctgac
      601 caaatcgaag actatgacga acgcatggtt gcgtatggta gagcgctggg ataccccgag
      661 actgcggttg aagcaaagcc cgacgaactt attaaatggt acgaacttcc ggataacgtg
      721 gtattcaacg aagaggttca ttcttctccg tttggcatgt cgagagagca ttggcaagaa
      781 gaaatcgaaa cagcaagaga atgggcaagc gttgcaaaag aagctgctcc acgtttgtac
      841 gaacaggcca caaaccaaat tttgcgcgat cggctgttat atgcgttaag cgaaacccca
      901 gaagaactgc gaaggatttt tcggtcgcaa gaggctcggg ccgcgctcga acaacgcaac
      961 aaacctctgt acgacaactt tatgcgcggc gcaagagcca acggcattat cgactaaccg
     1021 acgccgcggg gggagttgtt ttgctaacag tttgccggga gaatcgttat actgaaagaa
     1081 gagcttatgt ttgaaaacgt cataaactac gttgaccttc tcgttattgg ccttggcata
     1141 ctctatatac tctctgcggt gcaggcgggg gtttttagcg tattagggag gcttgtagcc
     1201 ttttttggag ctttggcaat tgctttttgg gggtatagtt atgcgcaaga cctttttgtg
     1261 gagcaatttg ccatgccctc gtcttttgcc agggctattg ggtttctttc tttgtttttc
     1321 ttcacccaat ttcttttaaa ccttatattc gggctggtgt tttctttcat accagagaaa
     1381 atgcgttatg catggtggag caaagttctg gctttggcgc cggctgttgt ggacgctgcc
     1441 gtgctggccg gagtaatact tatatttatt gtgctcttgc cttttgcgcc caaggctgcg
     1501 caggatatta ccgagtctcg aagcggcgca tttgctttgg aaaccatgtc gcgcctggaa
     1561 gggtacgcca accaggtatt cggcgaagct ttgcaggaca ctattacatt tcttaccgtt
     1621 cagcccgaaa ccgcagaacg cgtagaaata ccttacaaac ctgcggcttt gcacgtagac
     1681 gaaggcgcgg aagaacaaat gctccgctta gtgaatgaag aacgcgcacg ggcgggagtt
     1741 agcgcccttg taacagacga ggaaattgtg caggttgcca gagcacattc aaaagacatg
     1801 tgggagaaag gatatttttc gcacataaat ctcgagggca aagatccgtt cgatcgcatg
     1861 cgggacgagg acatcgagtt ttctgcggca ggagaaaaca ttgccttagc ccccaccacg
     1921 gcgctagcgc atcgaggcct catgaattca cccgggcatc ggaggaatat tttggatccc
     1981 aaattccagc gcgtgggcat tggcgttatc gacgggggag tgtacggcaa aatgtttact
     2041 caaaactttg cgctttaata catgtttggc attccttacc caaaaaagat actctttgct
     2101 ctggtcttag tttttatttt ctgcagttct tttgcggcat tgtggtcgct tgttgggtcg
     2161 catacgctcg gctgggacga agcagtgtat ctcaccaaag ctcgatcgtg ggcagaaggc
     2221 acccctgccg atgagtttcg gccatacaga cctattgcca tgcccgcgtt tgggtggata
     2281 tttcttcact ttggcgacac ggaagaaata ctccgcctct ttggcgctct ttttggcgcc
     2341 gtaacgctgg tatttctctt tttgcttttt cgcagcataa caaactcctg ggttgcctta
     2401 agcattgttt attcggttgg cacctcttct ttgtttttgc agcaggcgcc tcaatttctc
     2461 aatgatatcc cgtcgtctgg attgctgttt ggaattctct ggctcatttg gaaatactat
     2521 aaaacagagg gagaaaacaa atctattctc cttgcagcgc ccttagccgc tcttgctttt
     2581 taccttcgct acggtgttgt gctttccctg ggctttattg ggatagtatc gcttcttctc
     2641 ttatggcgga aattcgcagg aaaagagggt acaaactttt ctgttcttgc caagacgttt
     2701 gctgtttttg ctgcatttat gctgccgcac ggtatccatt ctttcattgt cgaagggagt
     2761 gcgctgggaa ttttacgttc gggtagcacg gctggcggcc gcgaatactt aggcgaaggt
     2821 ttactaaact atatccaatg gctgcccagc gcgcttgctg gccctttggt gggcgccagc
     2881 actattcttg gtatcgctgt tactcttgct attatctttc gaagaaattt gcgccagaac
     2941 tacgaaggct tgctatggct cggcagcatt gggatgttaa gcttcttttt taccggctta
     3001 ttggtacatg ccgaagcacg atatgttttc tttcctgtgg cgctgctttc cggggtaggt
     3061 attgcggggg tatacttcct tttgaaaaag gtgcggctgc aggtggttgg catcgggctt
     3121 ttcgcattag ccacactttt gtttggcgtg ttcaactatc aagaggcagc caccatcgta
     3181 aaagaaaaag agaccgacat atcgcgcaac gaatacaggg agacatacag agccattctc
     3241 cagaacaaac cgaaagaaga aaagtgcgtt gtgtggtcga tagactacct tccgcaaatt
     3301 tcgtggtata cacagtgcaa caccctaaga attgccgaga caaaaaacat agtcgtattc
     3361 ccgaatgacc cccttgtaac tccaaagggc gcgcattata gtattgtttt tacaaaatca
     3421 gaagacgggc aaattcagcc agacaacgct gaacgatacg gcgcattcct tacccaaatc
     3481 catcgatctc gggaatttcc cctttttatt ggccgcggcc aggcaaccgt gtaccgcatg
     3541 caacccaaaa aatccccggt caggcaaata accccctagc taagaatttc gttgacttat
     3601 ccacattgta ttttggacta gacggcacta tgctgtaaaa taaagaggag gtcgacaaac
     3661 agcaaagttt ctaaagaaaa gtaacccata tactacctaa tctaatgaag aaaataaaga
     3721 aacacgttaa aaaccaccaa ggccttattg ttctttctgg tattggtatt gttggtttgg
     3781 ctggcgcatt tgcctttatg gcttctgcag ccaccgactg tatgttcacc gaccagggca
     3841 acaccagaat gcttaacgca gattgcgcaa ccgacgctac catctttatt ccagatggtt
     3901 ttactttaga tgggaggcac cataccatta ccgcggttga tccaacggga gaccatttca
     3961 aaggagctgt ggtgcagaac gcaggggcaa acgcgcatgt gaaaaaccta gtcgttacta
     4021 ccagcaatct ctcgaatgca tgcgatgcgg ggggagaccg actccggggc attatgttcc
     4081 aaggagcatc gggttccatt gtgcacaaca cggtgtacaa cctgaaccag ggagcaagcg
     4141 gctgccagga agggaatgcc attgaggtgc gaaatgaacc cttcgacggc acccaccccg
     4201 acacacaaac tgttgaaatc gcccacaacg gtttaaccag ctggcagaaa accggcattg
     4261 ttgcaaacgg tgacgtaagc gtggatattc accacaacta tgtgggttct agcgccactc
     4321 aacccaacga tttggcggca aactcgatac agcttgggtt tggcgcaata ggccaggttg
     4381 tgcaaaacag gctcgatgga aaccagtggt gcggatcttc tgaatttgtg gctaccgcta
     4441 tcttactatt ccaaacgtca aacggtgcgg ttgtaaagca gaacattatc ggcggaaact
     4501 ctgacgtggg aatttacggc tttggagacg accttgtagt ggacaacaac aagctcacgg
     4561 acaacacagt tgaagattgc aaccagtttg cttacgacat tggcattgga aactatggaa
     4621 gtgcagcttc cctaagcagc ttcacaaaca acaaagtaaa cgggttcgat accccgtacg
     4681 acggcgttac gagcgggaag aacaaggtta tcccagggcc gcaaaagggc caaacctttt
     4741 tctaggaaca ggctgattaa agaacaaagc cgacgttacg tcggctttgt tttatttctt
     4801 ggagggcttg gactcggttt caatggcaat aagctgctcc atgcggcgga tatcggccaa
     4861 aagctccgac cggtctttgc taaacaagaa aatattaaaa atgcttttgc cgccgttttt
     4921 ggcgaacacg gcccgatcgc cgattttact gtttacatcc atggaatgaa aagaacccag
     4981 ctcctgcact ttttttacgc cagttaagtt ggtaataatg ccttcttgtt tggcgaaaaa
     5041 ttcaaacact gccgtgtgcc cgtatgtttt tcgggaaagc ttggggcgct cgccagcccg
     5101 cactgcaatg tcgtttgcgt agtggtgcat gccgtaagaa aggcgataca taaagtcgcg
     5161 aaatccgccc acgcgagggc ccagctcaat aattttccaa cccttttctg ttttcattaa
     5221 ttccagatgc acgcttgtat taaaaagggt gagggcccga atagcgtctg cgcttacgtc
     5281 tctcgccttg tacacgtttt cttttacgag gtcggtagga gttattctct ggtagccgaa
     5341 aaagtcgtca aacccaattt gtcttcctgt ggtaacatgc actaaagggc aaaaatatat
     5401 tttgccactt gtgtttacat acacgtctat ggagtacatg tcgccttcca taaactgctc
     5461 cactaacacc tggggttcgc cgcggccgcc agacctgcgg tattgagcgc ggatttttcg
     5521 aaaaatacgt ttgagcgact gctccaactc ttctgtgtga aacgcaatac tcacaagcac
     5581 tgaagccgcc aacccggagg gcttgataat aacagggaac cccacttgct gctcaatggc
     5641 ccgaagcgta gacttggtgg cgtctttggc aacaaaaaac tttggagtaa tgcgcggatt
     5701 gtacgcggca aaatgcttgc gcatttctat tttgcttgtt gcccagctca aagattccac
     5761 ggtgggtgtt cgcaaatgag gcacaaaggg tatgagccgc tggaaccacg gaataaaccg
     5821 ttcaaagcgc ggcacggtta ttgcagccag ttcttcttta taaggccaca gcgcctgttc
     5881 aacagactcg tcagagttga tgtcgcatgt aatggcaatg tcaaaaaggg cagcagcttc
     5941 cttttcttga tcagaaggct cctgatgata gagaaacgca taacggtatt tcttgccccg
     6001 ttttttccag ccttccgtaa gctgaaaagc gctttcgtag tctggcaaac caataataag
     6061 aacgattttt ttgcgtggtt ttgccgcagt ttccgtgttc atgaacggga gctggctttg
     6121 gtttcgattt tgagagcctg ctctatgcgg cggatatccg ccaacagcct ggaacgatct
     6181 ttgttaaaca gcaaaatatc gaacacgctt ttacccccgt tttttgcaaa cacggcgcgg
     6241 tcgccgattt tcttgttcgt ttccatggag tgcagggatt ctaacgcctg cacctttttt
     6301 acccccagga aattggtaat tactccttct tgcctggcaa atatttttaa tgccgctgcg
     6361 tatcctttga gcttgcgggg gagcttgggg cgctctggaa tacgcacggc aatgtcgttt
     6421 tcggtgtgct ttatgccgta ggctaactcg tagagcatgt ctcgaaaccc gccaattcgg
     6481 gggcccagct caataatttt ccagccctgc tcggttttca taagctccag gtgcaccgtg
     6541 gtcgagcgca agcccagcgc atgaatagcc tttttgcttg cttcctgcgc ttctgcaata
     6601 ctttcttttg caagaagggt aggggtcatt tgctggtagc cgaaaaagtc gtcgaaccca
     6661 atggtgcggc cggttttaat atgcactaac ggacagaaat acattttgcc tcgagaatcc
     6721 acgtaaatat caatggagta catgtcgcct tccataaact gttccactaa cacctggggt
     6781 tcaccgcggc ccccgttctt tttatattgc acccgcaaat gccggaatac tttacgaagc
     6841 gatttttcca gttcttcctg atggaaagca attgtcacca gcaaacttac ggcaaggccg
     6901 gaaggtttaa taacaagggg tgttccgatt ttctcgacaa tcgcttttat tgtctttttg
     6961 cttgtatcgg ataccaccat gtaggatggt gtaatctttt tgtcgtacgc cgtgaatctt
     7021 cggcgcatct ctattttgct cgtggcccat tctaaagaat cctgcgtggg cgtgcgcaaa
     7081 tagggaagat aggggataac ttttttcaaa tgcggaatat tttgctcgcc tcgggcggta
     7141 acagcaacaa acccatcctg atagggcgcc aagcactcca taatagactc tggcgaatca
     7201 aaattgcagg taagcacgat atcaaataaa tgcaaaactt ccttttcttc gtcgagaagg
     7261 gttttgttct gcggcatcat caccgcgaag cggtattttt gtttgcgatg cttcccaaaa
     7321 gcgagaatcc gctttacgac atgaagatcg ttgggcagcc cgattatcag gataacgttt
     7381 ttcctggctt tgcggggttt ttttgtcatc gatgtgaatg ttccatagtg gcaattttag
     7441 gcctttttgt caatgacgct ttttgctata attgatttca tgatacgcgc aatttttcta
     7501 tcaatactct ttctcttagt gggaattggt attggagcat acctgttttc tggcacccag
     7561 tcgcgctcgg ttttgcgcct gcaagaatgt agacaggatt gtctaagttc ccaagaaacc
     7621 ctggggctat tggcctctgt gggcattcaa aaaataggca gcgcgcttcc ttttattgta
     7681 aaagagaccg acaaaacgct ggccattcgc catcccgccc ccagagccga cgttcattac
     7741 gtaataattc ccaaacagga tatcaagaac ccgcacgacg ttacccagga agacgcgccc
     7801 tatcttgccg acgcctttgt cgtaatgcgg gagctcatcg aagaacaggg cctcgaggat
     7861 tacaaaatcg ttgccaacgg ccctggcttt caaaccgtgc aatacctgca ctttcacctc
     7921 gtctctgacc tcccggaata agataaataa aagagggcca ttacagccct ctcaatttat
     7981 tccacggaaa catccacgag ttcgagaaca tcttctggca cggtaatgcc cagttcttct
     8041 accgtgctaa aattcataaa aagcttctgc gtttgcgcaa actcgatagg gataccggct
     8101 ggttgtatgc cacttaacac cttctctacc tgtactgcgc tttggcgccc catttccaag
     8161 agatcatatt ggtatgtcat caaacctccg cttcgaactt caaccggagt tacccaaacc
     8221 gagggtatct tgagttcatt tatcctgtcg ataatgagct gcttgtcatc ttcatgtcca
     8281 agcagtgtcg cgccattgtg cagataggca tcaatgtcgc cgggtgctat ggcagctaac
     8341 gcctgctgca gctcttgcgt aagttgtggt ccggcttctt cagtttcgat aggataccac
     8401 acaatctcca aaccaagccg cggcgcttct atcgccaatt gggctttaat aaggttgagg
     8461 gcagtgcctg gctcgtggat gacatcaaaa acaccaatct ttttcgcctg cggagcaagt
     8521 cgtttcaaaa actccagttt ttttccggta agctcctgga agttcacctc aatacccgtg
     8581 aggttattcc cagatgattg atacgaatca ataatgccga ggtttatcgt attgagcgcg
     8641 tgcgacataa caataggaat atcggtacgg tttgccacgg cagtctcctc aaccgccacc
     8701 ttggcggaca cgcttgctaa ggcaaaaatc aagtcgacat cctgggctat cagttctttt
     8761 gccagcgcac gggttttttc ttcggtaaag cctacctcgc ttactgtata taccacatct
     8821 tgcccttctt catagcccgc ttcggccaag ccttgtttta agccatccac gatcgcgtcg
     8881 tgcacgggca aatagcgcag cacgccaatt gttatgggtt cttgcgcgct ctctttgggc
     8941 gcagttcctt gcaactggaa caacacaagg gctactgcaa aaaccaaggc aattgctgct
     9001 gcaccaaaca aaagcgattt cgagcgcaga ggatctttga ggggatcagg tttttggcct
     9061 tgttcttgcg ccagagtttc ctctgtatct tgaggattat ccattactct atagtgcttt
     9121 gtatgtacgg ccctgtcaag aaacttttcc cctaaactgg ttgacaaagc cccgtttttg
     9181 ccttatacaa agagtaggcc tcttcattat gcgtagagga aatttataaa gaggagtggt
     9241 ttcatgacta ccacagacac agaggttcgg gttagaggaa tcgggcacgt tggcattctc
     9301 gtggatgatc tgcatcgatc caagaagttc tacgaagaag tagttggcct ggaggaacac
     9361 aatagagttc ccaaaggatc cagcgaccaa gatgtccaga tgatcttctt cgggacgaaa
     9421 ggaggaaccc gtactaacgg agtccaccat gctctcgtca tcagacaagc gccagaagat
     9481 gttgatgttt cctcggctgt tccgttgacc aaaaaagcca ttcagcaaat cgccttccag
     9541 atgggtagcc gccaggactt cgaggatgcc ttggcccacc ttgcagcgaa cggtgtaccc
     9601 cttaccgagc gaacccggca cgatgaaacc gaattcgaca gtgggtcaga ttcggcatat
     9661 ttccaagacc cggacggcaa ccgcgtcgag ttcttcctca tgccctcctc ggaaacctag
     9721 aaccatctag gctaccatct tttataccta gcttccgcgc gagcgcaatt gcgggggctt
     9781 tttcttttat acaaatgtta tcattggcat tcctattcct cccgatattc tcgagatcgc
     9841 caacaccaca aattgacaat taactcgaat taggttatag aaagaacagt acgcaaggag
     9901 gtatcgcatg gcaacgccca aagaaatcat cagggatatc tactacccta ggcttttcga
     9961 tgggggagag ttgtgggtgg cagacgaaat cattgctccc gagtttttta acaacaggca
    10021 tcctacttgg ccgaggggac ccgagggcat taagaaagtg gtgcgcagtc tgcacaaaaa
    10081 atatgacggg gcgtttcatg cgctccacac agaaattctc cttcaagttg ccgagggcaa
    10141 cgtagtttcc accctcactc ggcaaacggg taagggagac gaagacatcc atcttgccga
    10201 acttcagtcg cacttccaca tcttcaacga cgcacagcta atcgttgagc atagggtcgt
    10261 atatatgcca ctgccgcaat aagcacggct ttctgaaacc agagggccgg ttttcttttc
    10321 tgtggagaaa aagcttggcc atatgggtga acatggtatg cttaatggat acaagcagta
    10381 acctcaaaaa ccatgccaca acattcaaca ctacttcgca gcgccattat cataattctc
    10441 attgcgggtc ttctgctcgt tgttgcagca acagttggga ttggcgcgat ttttatgcga
    10501 agcgactctc cgtctcccgg ttctcaagaa atgctgcgta taggagtagt gcaatacgcg
    10561 ccggtatcca atattcttac gcaaggattt aaacaaggca tggaagaact cgggtatgtt
    10621 gagggtgtac atgtagaata tgttgaaacg gcgcccacgc tcgacaatgc tctgctttcg
    10681 caaaatgcaa gagacatagc aagccaaaac gttgacctta tctttgccac caccgcagta
    10741 ggggggcttg ccgcgctcaa cgcaactgca gacattccta ttgtgtggac gcatgcagcc
    10801 acggccgtga caaacgggtt ggcggcgaac tataattctt ctggcaataa ttccacggga
    10861 attgaaattt cctttgccga gcttacccgg aaaaa
//
LOCUS       DASRUP010000110        11186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_409942, whole genome shotgun sequence.
ACCESSION   DASRUP010000110 DASRUP010000000
VERSION     DASRUP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409942"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..299)
                     /locus_tag="VFE94_04485"
     CDS             complement(48..299)
                     /locus_tag="VFE94_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50367.1"
                     /translation="MSLPKGWRRIESREELEEVLERKVTIKFVTPGLGKQKQQFEGAV
                     IGPSRDAGCFRFQITNGPTIKVEPGDLIVQESQPLEVVL"
     gene            complement(370..2364)
                     /gene="acs"
                     /locus_tag="VFE94_04490"
     CDS             complement(370..2364)
                     /gene="acs"
                     /locus_tag="VFE94_04490"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZX50368.1"
                     /translation="MTNQQERQKMREERTYYPAKELSDRAHIKNMEEYRRMYKESVED
                     PQAFWGKQAEKHLSWFKKWDSVLEYDFAKIGEVQGPYVQFFRGAKLNVSYNCLDRHVN
                     SWRKNKAAIIWQGEKEEDKRTITYQQLQRAVSRFANVLVKHGVQKGDTVTIFMPMVPE
                     AAVAMLACTRIGAIHSVVFSAFSAEALKNRILDCDSKFVLTCDIGFHGGKVIELKEKV
                     DEALQSCPDVKKVLVYKRGEKDAVMQKGRDLLWQEEVNAEDIQDTFTPAEMDAEDPLF
                     ILYTSGSTGKPKGVLHTTGGYLLYATLTCKYVFDLKDEDVYWCSADIGWVTGHSYVVY
                     GPLANGATVVMFEGTPTYPEPDRFWKIIEEYGVTIFYTAPTAIRALMRLGEEWPGKHD
                     LSSLRILGSVGEPINPEAWMWYYRVIGKECCPIVDTWWQTETGGIMITPLPGATPLKP
                     GSASFPFFGIVPEVLKDDGLEAPVNEGGRLVITKPWPGMIRGVYGDPKNELVKKVYFS
                     LYPGKYFTGDGSRKDESGFYWLLGRVDDVMNISGHRFSTAEIESALVGHESVAEAAVV
                     GVPHDIKGEGMHCFVTLKQGYESSPQLIEALIMQVRKEIGPIATPDRIQFAEQLPKTR
                     SGKIMRRILRKIAIGDTQDIGDTSTLADPSVVETLVANKV"
     gene            complement(2357..3859)
                     /locus_tag="VFE94_04495"
     CDS             complement(2357..3859)
                     /locus_tag="VFE94_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZX50369.1"
                     /translation="MQQDKLFYNGYFWQGENKWAGAVLVGENGAIQEVFQNAEEAEGK
                     MGGGVEKVDMQGQWVLPVFEDAHNHPTRFARLFFEIDFRGQNISWEEARELIRKQVAR
                     TPIGNWVVCHGWNEERWQSLRSEDLDDLSQEHGILLIDVSEHGGLLNKQGRFLLEQKG
                     LRVPGEFVEDELFEEVCRHTALSKDAYKKGIQKFHEKLLALGVGAVHDMDVSMPEELQ
                     AYEELLQEKKLVLPSALYLNPRLLGHTEQISRLLNYNDTFKVCGVKIFLDGAIGTRTA
                     ALSRPYEDAATSGMLRMKEKEVAAIAKKAAVLGLRHIAMHCIGDRAIDAAVAIQTKVQ
                     KNYPDMLWRYEHFEMPSKGAIATIAENNGVVSVQPNFSWDVENYQGRLGKRTEMINPL
                     QWIEESGATMVFGSDDMPSGPLEGISWAIQKAPFSHQKVSAASAVRAYTETPARIVGA
                     IRGKVVSGHEANFVVFRKNPLEAKNELENLAVEGVWLKGLIQDNQEEYYD"
     gene            complement(3907..4863)
                     /locus_tag="VFE94_04500"
     CDS             complement(3907..4863)
                     /locus_tag="VFE94_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50370.1"
                     /translation="MESLSIGAMNVLKYEPWNDRDKGFLSITHNSVEELASTLCDVVD
                     ECQEGSRVTVRVVVSEDYPVREYPKGICWQGVTLVPEAVVPICKDGVGAIVYFGANTD
                     QRIDGFVRDRVQDLLRIPEQEWFLIRQAEKAERKSKPLPDGYTVGILPKKVFQQDLQR
                     LLEIYQGSYSTYITELNARAVGEMVQNNTIAVVRNSWGSIVAVSQAEVALIELAEQDW
                     QLIELTEYAADPKAGGNGFCRMCNELLVQKLSGPHSIIYSETRANSGAALGSNPVPML
                     VGGRLPAHCKMDSTVKDVPQVGPYANLCVWYLPQGNSEKGAE"
     gene            complement(5226..5900)
                     /gene="rsmI"
                     /locus_tag="VFE94_04505"
     CDS             complement(5226..5900)
                     /gene="rsmI"
                     /locus_tag="VFE94_04505"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZX50371.1"
                     /translation="MGTLYIVATPIGNLEDITIRALRVLKEVDGILCEDTRVTKKLLD
                     RFDIHTATISYHQHSRLQKVEEILGLISQGKSIALVSDAGTPGISDPGNQLVQEVAEQ
                     LPEVSIVPIPGVSAVGALASVSGFPMDRFVFLGFPPVKKGRNKFFQEIVQAEYPVIFY
                     ESPFRIRKALTELQNMPNGKIVVGRELTKKFETIYRGTAEQVLAQLGKEQERGEFVVA
                     VWKSKT"
     gene            complement(5900..6934)
                     /locus_tag="VFE94_04510"
     CDS             complement(5900..6934)
                     /locus_tag="VFE94_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZX50372.1"
                     /translation="MSGNSQLLDISWATIVKLGLTGFLVYAVFSLRDLLVLVLFGVIL
                     SILFDPVIDFLQTKKIPRVVSTIGVYLLLFSFLSYVIFATTPIFVTEIQEFSHRLPQY
                     FEERINPSLEGLGVSTFKDFQSFVDVLTQKATEDTSTIFEALVGIFGGIFSTVFVISV
                     SIFLSIEDRPIERTITLLFPKKYEALALSLWKRSQRKVSGWFLSRVLSSIFVAAATYF
                     TLLLFGVHSPLSLSLLAGVLNFVPVVGPLLAGVLIALLVALESPLQALFVILAFVLIQ
                     QVEENVLMPLLSKRLIGVPPALVLISLAIGAHFWGIMGAILAIPLAGIFFEFLRDFLK
                     KQKEEKTVVL"
     gene            complement(6936..8201)
                     /locus_tag="VFE94_04515"
     CDS             complement(6936..8201)
                     /locus_tag="VFE94_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZX50373.1"
                     /translation="MIKTITLKSGLRVVTIPQRATKTVTVLVLVGTGSKYETKEQNGI
                     SHFLEHMFFKGTEKRVKPIDVVEPLDRIGGSYNAFTSEDYTGYYAKVDSSHFDLAFEW
                     VADIFLNSTLPAKEIQKEKSVITEELHMHKDNPMWYVDVLWEKLLYGDQPAGWEVVGT
                     RETIAAMARKNLLRYRKNQYVASNTVVCIAGNVRAPEVMAKVQKLFAGIPTREFQRKV
                     PIVEAQKKPEILLEYRKTDQSHIALGVRGYNLQDPRRYAQYTLATLLGGMMSSRLWVA
                     IREKNGLAYYVSTATDCNPDTGYVVTSAGVKDENVERAVRIILQEYAKVKKYPVSKDE
                     LQKAKDHQKGKMALQLENSDAKAHFYGMQELLQGNILTQEEICAKIDKVDSKDIQEIA
                     QDMFRPAKLNLVVLGPSRNKAQFTNLLQI"
     gene            complement(8291..9190)
                     /locus_tag="VFE94_04520"
     CDS             complement(8291..9190)
                     /locus_tag="VFE94_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZX50374.1"
                     /translation="MKLIDSTAQTSWKFLAIIAALAVFAVGGALLLHYSGIKTETPEQ
                     PGLPAEKQNEVSPLPTPKEIPPTELQEQSQTQPSPSPLPAWPTSLTLLSPRLGEAWLQ
                     TVAYTISWCCGTGNVDIFLYRWHWDTAVFDTGKDEEDPVLVIGENVSSKEGVERENGE
                     YIWQVPKNIEPGPYYIRIADSVSSYSRNEQFRIIPPMTKLERLAPVSGPVGSTIILHG
                     EGFMPSGNTVNFGTGAIPNVRAPNWHTLPSFILPNELMPGCFYEDPACAASPQPVTPG
                     EYTVSVTNDFGITRTLTFTVTEQ"
     gene            complement(9208..10209)
                     /locus_tag="VFE94_04525"
     CDS             complement(9208..10209)
                     /locus_tag="VFE94_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50375.1"
                     /translation="MNYLMNLLKGGMAGGVSGVIVGATFCGLILANLFFKATPIDWEL
                     GIVISLLLLALSIALGAVGGILFLSFLKGPSFVRAFYLIFLFFALSVFLSWFFVFPML
                     FTGWQNVGPWILGIFGVFAAAVVLFALLYRKIKQNLIWKGIFFIIPLALFPFFFLVLD
                     VYDVLALNRIITLRQGTFEGFGLLHGELPSGVWKNPIDIDLTVKNSYGLPLNYGSQSG
                     DLSGGGSEYMFVPNSQDLVISVDVSDLKAAATERGYQLSDIRYNATVKVEAQDLELKR
                     NTLLEENISQTLVDPPPMIRVEGNSTLLLPIKILIILLPLLWVLLMVYFFSRLKLAR"
     gene            complement(10469..>11186)
                     /locus_tag="VFE94_04530"
     CDS             complement(10469..>11186)
                     /locus_tag="VFE94_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005528455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HZX50376.1"
                     /translation="DDKWFDFWIDAWEKFYLDLGIKKENLRRYEHPKEKLSHYSKRTV
                     DLEYKFPFGWGELAGVANRTDFDLKQHAKFSGKELTYRDEESGEVYVPYVIEPTMGIE
                     RAMLAFLIDAYEEIEGGRTKTTESTKEKEVVLNLVKQLAPVQVAVFPLMKNKPELVAK
                     AKEVYQMLQKDFACQYDEAGAIGRRYRRQDEIGTPYCVTVDFDSLEKNDVTLRHRDTM
                     EQTRVKISDLAHELQTRFGE"
BASE COUNT         2948 a         2899 c         2317 g         3022 t
ORIGIN      
        1 ctttattttc cagaaaaata aacctcggga aatgtgcttc ccgagtctta gagaaccacc
       61 tccaaaggtt ggctctcttg aacaatgagg tctccaggct ccaccttaat ggtgggtcca
      121 ttggttattt gaaaacggaa acaccccgca tctcgagagg gcccgattac tgccccctca
      181 aactgctgtt tctgcttacc caggccaggc gtaacaaatt tgatagttac ttttctctct
      241 aacacctctt ccagctcttc cctagactca atgcgacgcc acccctttgg caaagacatg
      301 ccagcacctc ttttattgta gtccgctttc tatttaacga aaatatgggc ttcttgtcaa
      361 tggggaaaac tatactttgt ttgcaaccaa agtctctacc accgaagggt cggccagagt
      421 gctggtgtcg ccaatatctt gcgtgtcgcc tatagctatt ttgcgcaaaa ttctgcgcat
      481 aattttgcca gagcgcgttt tgggcagctg ctctgcaaac tgaattctgt ctggagtagc
      541 aatagggcct atttcttttc tcacctgcat aattaaggcc tcaatgagtt gcggagaaga
      601 ttcgtatcct tgttttaatg taacaaaaca atgcattcct tcgcctttga tatcatgcgg
      661 aacgcccact acggctgctt ccgccacgct ctcgtggccc accaaagcgc tttcgatttc
      721 tgcagtggaa aaacgatgcc ccgagatatt cataacgtcg tctaccctgc ccagcaacca
      781 gtaaaatccg ctttcgtctt tccggcttcc gtcgcccgta aagtattttc caggatacaa
      841 agaaaagtat actttcttga ccagttcatt tttagggtcg ccgtataccc cacggatcat
      901 tccgggccag ggtttggtaa ttaccaatcg tccgccctcg tttaccggag cttcgaggcc
      961 gtcgtctttg agaacctctg gcactatgcc aaaaaaagga aacgaagcgg aacccggttt
     1021 taaaggcgta gctcccggaa gaggagttat cataatgccg ccggtttctg tttgccacca
     1081 tgtatctaca atggggcagc attctttgcc aatgacacgg taataccaca tccaggcttc
     1141 ggggttaatg ggctctccca cgctccccaa aatgcgaagg ctcgataaat cgtgtttgcc
     1201 tggccactct tcgcccaaac gcattaatgc gcgaatggcg gtgggagccg tataaaatat
     1261 ggttacgccg tattcttcga taatcttcca aaagcggtcg ggttcaggat atgtgggcgt
     1321 accctcaaac ataactaccg tagctccgtt tgcaagcggc ccatatacca cgtaggaatg
     1381 ccccgtaacc cacccaatgt ccgcagaaca ccagtacacg tcttcgtctt tcaaatcaaa
     1441 cacatatttg caggtaaggg tggcatacag caaatatcct cctgtggtat gcagcactcc
     1501 ttttggcttg cccgtgctgc ccgacgtata cagaataaac aaggggtctt ctgcgtccat
     1561 ttcagcagga gtaaaagtat cctgaatgtc ttctgcattc acttcttctt gccacaagag
     1621 gtcgcgacct ttctgcatga cagcatcttt ttcgccgcgt ttatacacca gcaccttttt
     1681 cacgtcgggg cagctttgca aggcctcgtc tactttttct ttgagctcga ttactttgcc
     1741 tccgtgaaag cctatgtcgc aggtgagcac aaatttggaa tcgcaatcaa gaattcggtt
     1801 tttgagcgct tccgcagaaa atgcagaaaa caccaccgaa tgaatagcgc cgatgcgcgt
     1861 gcatgccaac atggctaccg cagcctccgg aaccatgggc ataaaaatgg taacggtatc
     1921 tcctttttgt acaccgtgtt ttactagtac atttgcgaac cgagataccg ctctctgtaa
     1981 ctgctgatac gtaatcgtac gtttgtcttc ttctttttct ccctgccaaa taattgctgc
     2041 tttgtttttg cgccaggaat tcacatgccg gtctaaacag ttatacgaca cgttaagctt
     2101 tgctccccta aaaaactgca cgtatgggcc ctggacctct ccgatttttg caaaatcata
     2161 ttctaaaaca gaatcccatt ttttaaacca agaaagatgt ttttccgcct gctttcccca
     2221 gaaagcctgg gggtcctcca ctgactcttt gtacattcgc cggtactctt ccatgttttt
     2281 tatatgagcc cggtcgctca actctttggc cggataatac gtcctttctt cccgcatttt
     2341 ctgcctttct tgttggttag tcatagtatt cttcttgatt atcttgtata agccctttta
     2401 accaaacccc ctcgacagca aggttttcaa gttcattttt agcctctaac ggattcttcc
     2461 tgaatacaac aaaatttgcc tcgtggccag acaccacctt cccacgtatt gctcccacaa
     2521 ttcgggccgg cgtttcggtg taagcccgca cagcgctggc cgccgagacc ttctggtggg
     2581 aaaaaggtgc cttctggata gcccaggaaa tgccttctaa aggccccgaa ggcatatcat
     2641 ctgatccaaa caccattgtc gctcccgact cctctatcca ttgcagcgga tttatcattt
     2701 ctgttctctt tccaagacgg ccttgataat tttccacgtc ccatgaaaaa tttggctgaa
     2761 ctgaaaccac tccgttgttt tcggctattg ttgcaattgc gcccttactc ggcatttcaa
     2821 aatgttcgta cctccacaac atatccgggt aatttttttg cacctttgtt tgtatagcaa
     2881 cggccgcgtc aattgctctg tcaccaatac agtgcatagc gatatggcgc aagccaagga
     2941 ccgctgcttt ttttgctatg gcagcaacct ctttttcttt catgcgcagc ataccgctgg
     3001 ttgccgcgtc ttcatacggc cggcttaatg cggccgttct cgtaccaatt gccccatcga
     3061 gaaaaatctt tacaccgcaa actttaaacg tatcgttgta gtttagaagc cgtgaaattt
     3121 gttcggtatg ccccaaaagc cgcgggttca aatacaaagc gctgggcaat acaagctttt
     3181 tctcttgcaa aagttcttcg tacgcttgca gttcttctgg catagacaca tccatatcat
     3241 gcactgcgcc gactcccaaa gccaaaagtt tttcgtgaaa tttttgaatg ccttttttgt
     3301 acgcgtcttt cgaaagcgcc gtatgccggc atacttcttc gaaaagctcg tcttccacaa
     3361 attcgcctgg tacacgcagc cctttctgtt cgagcaaaaa tcttccctgc ttgtttaaga
     3421 ggccgccgtg ttctgacacg tcgatcaata aaatcccatg ctcttgcgac aaatcgtcta
     3481 aatcttcaga gcgcaggctt tgccaccgtt cttcgttcca gccgtggcac actacccagt
     3541 tgccaatggg cgttctggcc acctgcttgc gtatgagctc ccttgcctct tcccaggaaa
     3601 tattctggcc ccgaaaatct atttcaaaaa acaggcgcgc aaatcgggta gggtggttgt
     3661 gagcgtcttc aaagaccggc aaaacccatt gcccttgcat atcaactttt tcaacgccac
     3721 cacccatttt gccttcggcc tcctctgcgt tttggaaaac ctcttgaata gcgccgtttt
     3781 ctccaaccaa aacagcgcca gcccatttgt tttcgccttg ccaaaaatat ccgttataga
     3841 aaagtttgtc ctgctgcatc ttaaaagtat caaaaaaaga taacgggagc gaaagtgagc
     3901 gctagctcac tccgctccct tctcactgtt cccttgcggg agataccaca cacacaagtt
     3961 ggcatagggc cccacttggg ggacatcttt tactgttgaa tccattttac agtgtgctgg
     4021 aaggcgtccg ccaacaagca ttggaacagg gtttgatcct aaagcagctc cactgttcgc
     4081 ccgcgtttca ctgtaaataa tagaatgagg gccagagagc ttctgaacaa gcagttcatt
     4141 gcacattcgg caaaagccat tgcctccggc tttgggatcg gcagcatact ccgttaactc
     4201 tatgagctgc caatcttgct cggccagttc gataagtgcg acctctgcct ggctaactgc
     4261 cacgatgctt ccccagctgt tgcgtacgac tgctatggta ttgttctgaa ccatttcacc
     4321 cacagcccgg gcgttaagct cagtaatata ggtggaatag cttccctggt agatctccaa
     4381 caaacgctgt aagtcctgct ggaaaacctt tttgggaaga attccaaccg tataaccgtc
     4441 tgggagtggc ttggactttc gctctgcctt ctcggcttgg cgaataagaa accactcttg
     4501 ctctgggatg cgcagaaggt cttgaaccct gtctctaacg aagccatcta tcctctggtc
     4561 ggtgttggct ccgaaataga ctatggcacc cacgccatcc ttgcatattg gaaccacggc
     4621 ttccggtacg agcgttaccc cttgccaaca aatccctttg ggatactcac gcacagggta
     4681 atcctcgcta acaaccaccc gaacagttac ccgtgagcct tcttggcact cgtcgaccac
     4741 atcacagaga gtgctggcca attcttcgac cgagttatga gtgatagaaa gaaagccctt
     4801 atcgcgatcg ttccaaggtt catactttag tacattcatc gccccaatgc ttagagattc
     4861 catttctcac ctctacaatt cccaaatttt gagcagtatt gccaatgttt gtgtgtgtta
     4921 tgtgctttga gaggaagcaa ggcaagcttt cttcctcatt ccaacacaaa acaaaaccgc
     4981 cttctggtag ctccaagaag acggctcaaa gtttttgctt tatgaaaaaa cgaaaactac
     5041 catcttcttg aagatgcggt ttccgctttt ttggtttcgg cggccaggcc cagaggcact
     5101 ttttctaacc ccctgcagca acgataactc ctcccccatc tggaagagga ttgcgcacta
     5161 cctataatat ctgaaacttc gtatgccatg atatatattg atagtaaaag aattggcgct
     5221 tttggtcaag ttttgctttt ccacacggct acgacaaact cccctctctc ctgttccttg
     5281 ccaagctgcg caagtacttg ctctgcagtt ccacggtata tcgtttcgaa ttttttggta
     5341 agctcgcggc ctactacgat tttcccatta ggcatgttct ggagttcggt taaagccttt
     5401 ctaattctaa atggcgactc gtagaaaata accgggtact ctgcttggac aatttcttga
     5461 aaaaacttgt tgcggccttt ttttaccggc ggaaacccca aaaatacaaa cctgtccata
     5521 ggaaatcctg aaacgcttgc caatgccccc accgcagaaa ctccgggaat cggaacaatc
     5581 gaaacctccg gcaattgttc tgcaacctcc tgcaccagct ggtttcccgg gtctgaaatt
     5641 ccgggagtcc cagcgtctga aaccagggca atgcttttcc cttgcgagat caacccgaga
     5701 atttcttcta ccttctgtaa cctactgtgc tggtgatagc ttatcgttgc agtgtgaata
     5761 tcaaaacgat caagcagttt ctttgtgaca cgcgtatcct cgcacagaat accatccacc
     5821 tctttgagca cgcgcaatgc ccgaatcgta atatcttcca aattgcctat gggcgtggca
     5881 acaatataca aagttcccat tataaaacaa ccgttttctc ttctttctgc tttttcagaa
     5941 aatcgcgcag aaattcaaag aaaataccag ctaaaggaat tgccaaaata gcgcccataa
     6001 tgccccagaa atgcgcccca atcgccaaag aaatcaacac aagggccggc ggcacgccga
     6061 taaggcgctt agacagcagc ggcattaaca cgttttcttc cacctgctgg atgagcacaa
     6121 aggcaagaat aacaaagagc gcctgcaaag gagattctaa agcaacaagc aaagcgatta
     6181 atacgccggc taacaacggc cccaccaccg gcacaaaatt cagcacccct gcaagcaagc
     6241 ttaaagaaag aggtgagtgc accccaaaga gcaaaagcgt aaagtaggtg gcggccgcaa
     6301 cgaaaataga actcaacacg cgcgataaaa accatcccga aacctttcgc tgcgagcgct
     6361 tccacagact taaagccagt gcctcgtact ttttcgggaa cagcaacgta atggtgcgtt
     6421 cgatgggacg gtcttctata gaaagaaaaa tggaaaccga aatcacaaaa accgtagaga
     6481 aaatgccgcc aaaaatgccc acgagcgcct caaaaatcgt agaagtgtct tcggtggctt
     6541 tctgggttaa gacatcaaca aaactttgaa aatctttgaa ggtgcttacg ccaagaccct
     6601 ctaaggaagg gttaatccgt tcctcgaaat attggggaag ccggtgagaa aattcctgga
     6661 tttcggttac gaaaatcggg gttgtggcaa aaatcacata cgaaagaaaa ctaaaaagca
     6721 gcaggtatac ccctatggtg gaaaccacgc gaggaatttt ttttgtctgg agaaaatcga
     6781 taacggggtc gaacagaata gaaagaataa cgccaaaaag taccaatacc aaaaggtccc
     6841 gtagcgaaaa aaccgcatac accaaaaaac cggtaagccc caacttcacg attgtcgccc
     6901 atgaaatgtc gagcaattgg ctgttcccgc tcatgctata tttgcaaaag gtttgtaaac
     6961 tgcgctttgt tgcgagacgg ccccaaaacc acaaggttca gttttgcggg ccgaaacatg
     7021 tcctgcgcga tctcttgtat gtctttggag tccactttgt ctattttagc acagatttct
     7081 tcttgcgtta aaatgttgcc ctgcaaaagc tcctgcatgc cgtaaaaatg agctttggcg
     7141 tcggaatttt ccagctgcaa agccattttc cctttctggt ggtctttcgc tttttgaagc
     7201 tcgtctttgg aaaccgggta ttttttcact ttcgcgtatt cctgcagaat aatacgcacc
     7261 gccctttcga cattttcatc tttcacgcca gccgatgtta ccacgtatcc cgtgtcggga
     7321 ttacagtctg tcgcggtgga aacgtaatag gccagcccgt ttttttcccg tatcgcaacc
     7381 caaagccggg aactcatcat tcctcccaaa agcgtggcaa gcgtgtattg cgcgtatctc
     7441 ctggggtctt gcaagttata ccctcgtacg ccaagagcaa tatggctttg atcggttttg
     7501 cggtattcca aaagtatttc gggcttcttc tgggcctcga ctatcgggac ttttctttga
     7561 aattcccgtg tcggaatgcc ggcaaaaagt ttctgcacct ttgccattac ttcgggcgct
     7621 cgcacattcc ccgcaatgca tactaccgta ttcgaggcta catattggtt cttccggtag
     7681 cgcagaagat tcttcctggc cattgcggca atcgtttctc tggttcccac cacttcccag
     7741 ccggcaggct ggtcgccata caacaacttt tcccaaagaa cgtctacata ccacataggg
     7801 ttgtctttat gcatgtgcag ctcttcggta atcacgctct tttctttttg gatttctttt
     7861 gcgggaagag tagaattcaa aaaaatatcg gccacccatt caaacgccaa atcaaagtgg
     7921 ctgctatcca cttttgcata gtagcccgtg taatcttccg aggtaaacgc gttgtaagaa
     7981 ccgccaatac ggtctaatgg ttccaccaca tcaatcggct ttacgcgttt ttcggttccc
     8041 ttaaaaaaca tgtgctctaa aaagtgcgaa atgccgtttt gttctttggt ttcgtattta
     8101 gagcccgttc ccaccaacac taaaacggtc accgttttgg tggcacgctg cgggatcgtt
     8161 acaacccgta atccactctt gagcgtaatt gttttaatca tcgatatctt acgctagcac
     8221 tttttcgcga aaaacaaaag ccctcgaaac tccatgtaag aattcccgcg ggcagtgttc
     8281 tatacttact ctactgttca gttaccgtaa aggtcaaagt ccttgttatg ccaaaatcgt
     8341 tggtaacgga tacggtatat tctcctggtg taacaggttg aggagacgcc gcacatgccg
     8401 gatcttcata aaaacatccg ggcattagct cgtttggcaa gataaaactt ggcaaggtat
     8461 gccaatttgg agcacgaaca ttgggtatcg cccccgtccc aaagttaacc gtattccccg
     8521 acggcataaa cccctctccg tgaagaataa ttgtgctccc aaccggccca gaaacaggag
     8581 caagcctttc gagtttagtc atcgggggta taatccgaaa ctgttcatta cgactatacg
     8641 aagaaacaga atcggctatt cgaatgtagt aggggcccgg ctcaatgttt tttggaacct
     8701 gccaaatata ttccccgttt tctctttcaa caccttcttt tgacgaaacg ttttctccta
     8761 tgacaagtac ggggtcctcc tcatctttgc ccgtatcaaa gacagccgta tcccaatgcc
     8821 aacgataaag gaaaatatca acgtttcctg ttccacaaca ccaactgata gtataagcta
     8881 ctgtttgcaa ccaggcctct ccaagacgcg gagaaagaag cgtaagagaa gttggccatg
     8941 caggaagtgg tgatggagac ggttgagttt gggattgttc ttgcaattct gttggtggta
     9001 tttcttttgg ggttggtaac ggtgaaactt cattttgctt ctcggcaggc aatcctggct
     9061 gttccggcgt ttctgttttt atgccggaat agtgcaaaag caaagcgcca ccgacagcaa
     9121 atacggcaag ggccgcgata attgcgagaa acttccaact tgtttgcgcc gtggaatcaa
     9181 taagtttcat gtagtccgta gaaacattta tcgtgctaat ttcaatcgag aaaagaaata
     9241 aaccatcaat aaaacccaaa gaagtggcag aagaattatc aagattttaa ttggaagcaa
     9301 taacgtagag tttccttcca cccgaatcat aggaggagga tctactagtg tctgggagat
     9361 attttcttcc aaaagcgtat tcctttttag ctccagatcc tgtgcctcta cttttactgt
     9421 tgcgttatac cgaatatcag acaactggta tcctctttca gttgccgcag cctttaaatc
     9481 cgacacatcc acactaatca ctaaatcttg ggagtttggc acaaacatgt actcactgcc
     9541 tccaccagac aaatcaccac tttggctgcc ataattaagc ggcagcccat aagaattttt
     9601 tacagtcaag tcaatgtcga ttgggttttt ccaaacacca gaaggtagtt ccccatgaag
     9661 aagtccaaac ccctcaaatg ttccctgcct taaagttatt attcgattta gtgccaaaac
     9721 atcatataca tctaacacaa ggaaaaagaa aggaaacaag gcaaggggaa taataaagaa
     9781 aattcctttc caaataagat tttgttttat tttccggtat aaaagggcaa ataaaaccac
     9841 ggccgccgca aaaaccccaa aaattcctaa tatccacggg ccaacgtttt gccaccccgt
     9901 aaataacata gggaatacaa aaaaccaaga aagaaaaacc gaaagagcga aaaagagaaa
     9961 tataaggtaa aaagccctca caaaagaagg gcctttaagg aaagagagaa ataatatgcc
    10021 tcctactgca ccaagcgcta tacttaaggc cagcaataaa agagatataa caatccctag
    10081 ctcccagtct atgggggtcg ccttgaaaaa taagttggca aggattagcc cacagaaagt
    10141 agctcctact ataactccac ttaccccacc cgccattcct cccttgagta aattcataag
    10201 ataattcata tacacggtcg ataacaatca ccaaaaatta gtgaaaaata gctcgtccga
    10261 gaaataagaa aacaggaatt ccgattgtaa taaccagagc tataagaatt cccttaagcg
    10321 gccgcttttt agcaatagaa aagatcagta tccctatctc taccaaagcc gcccagggaa
    10381 gaatgattaa cgagataacg aatagccagt cgaaaataat ggttacggtt tccattacat
    10441 aagtataact tttggaaaca atccttgtct attctccaaa ccgggtttgg agttcgtggg
    10501 caaggtcgga gatttttacg cgggtttgct ccatagtgtc gcggtgccga agggtgacat
    10561 catttttttc gagagaatcg aaatctacgg ttacgcaata gggcgtgccg atttcgtctt
    10621 gcctgcggta gcgccggcca atggctcctg cctcgtcgta ctggcatgca aaatcttttt
    10681 gtagcatttg gtacacctct tttgcctttg ccacgagctc tggtttgttt ttcattaagg
    10741 gaaataccgc aacctgcacc ggcgcaagtt gcttaaccag gttaagcaca acctctttct
    10801 ctttggtgga ttctgtggtt ttggtacgac caccttctat ctcttcatac gcgtctatga
    10861 gaaatgcgag cattgccctc tcaattccca tagtgggttc tataacatag ggtacgtaca
    10921 cctcgccgct ctcctcgtcg cggtaggtaa gctctttgcc cgaaaacttt gcgtgctgtt
    10981 ttaggtcaaa gtcggtgcgg tttgccacac cagccaactc tccccaccca aacgggaact
    11041 tatactccaa gtctaccgta cgcttggaat aatgcgacag cttttctttg gggtgctcat
    11101 atcttcgcaa gttttctttt ttgattccta aatccaaata aaacttttcc caggcgtcta
    11161 tccaaaaatc aaaccatttg tcgtct
//
LOCUS       DASRUP010000111        16433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8136
            k141_444100, whole genome shotgun sequence.
ACCESSION   DASRUP010000111 DASRUP010000000
VERSION     DASRUP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970183
            Sequence Read Archive: SRR3989344
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 929
            CDSs (total)                      :: 881
            Genes (coding)                    :: 876
            CDSs (with protein)               :: 876
            Genes (RNA)                       :: 48
            rRNAs                             :: 2 (16S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16433
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444100"
                     /isolate="SMAG_U8136"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..761
                     /locus_tag="VFE94_04535"
     CDS             51..761
                     /locus_tag="VFE94_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50377.1"
                     /translation="MSTKVFYRVVACVVSFVFLWGATSYAEAQTTTTGTGFEVAQKVR
                     NLTQQKFDWVDKVQASPQDRVEFQITVTWRGSATTENVLVREILADKLSYAGNLRVDS
                     NQVAGNVTTENINIGSLNTNQARTITFETLVVDAASLPAGTLNLVNTVTVFNTQGGAS
                     TTAQVQVTRDGKPTDVSTGPLSLWQIGFALMLLVMGVASAAFFAKYYFTREVLQSPYE
                     NRTDRKLASMIEKGKRQS"
     gene            835..1131
                     /locus_tag="VFE94_04540"
     CDS             835..1131
                     /locus_tag="VFE94_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50378.1"
                     /translation="METFVKADIFFFVTTVAIFLVTILLAVALVYAIRILRDVQHMVR
                     RAKEESDGLFEDISQLRAFIREKGQKVGAVHEIVKGIGKLVSSRKKPAKSSKKA"
     gene            1337..2536
                     /locus_tag="VFE94_04545"
     CDS             1337..2536
                     /locus_tag="VFE94_04545"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZX50379.1"
                     /translation="MNKIFFAILGVFVVAVGAFAGFTYWQQFRGAGPAILPPGDDIGE
                     IIDETPPEGQNNTDFPLKLPDGFSISVFAKDLPGARVMAFDGFGNMWVSQTSEGKISL
                     LEVEDGKVTKQYPILQDLRRPHGLAFHPQQPFTLYFAEEDSVSKIPVYSDAPSQKLFD
                     LPAGGGHFTRTILFAPDGRLLTSVGSSCNVCHEEDWRRASVLVSDDEGKNLKTFARGL
                     RNAVFMATNYVTGDVWVTEMGRDNLGDDIPPDEINILQEEGNFGWPICYGKNIHDTNF
                     DKNTYIRAPCEEQFGEIPSHVDLQAHSAPLGLAFVPEEGWPEEYWYDLLVAFHGSWNR
                     SEPTGYKIARIKLDAQGNYEGQEDFITGWLQGNQALGRPVDILVQPGGVMYISDDKAG
                     LIYRVTL"
     gene            complement(2543..2971)
                     /locus_tag="VFE94_04550"
     CDS             complement(2543..2971)
                     /locus_tag="VFE94_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50380.1"
                     /translation="MPRFLLLPLFALLYIASAPLSVDAQSLVPCGGPGQPPCGLCALF
                     ELVHNIITFLLVPSAFNGGLPIVLALAGLLFAVGGFFMLTSAGNMSNLQRGRTVIFST
                     VVGLLIVYGSWIFINLLLATFGVTTFTGTTTWWQVECSLP"
     gene            3062..4099
                     /gene="metK"
                     /locus_tag="VFE94_04555"
     CDS             3062..4099
                     /gene="metK"
                     /locus_tag="VFE94_04555"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZX50381.1"
                     /translation="MPNFYTVESVTSGHPDKVCDQISDAIVDACLEQDPRSRVAMETF
                     GAHGMLVVGGEITTNANVDYAQIARNVYKEIGYDNDLEIITRVVRQSPDIAQGVDTGG
                     AGDQGIMYGFATQETPEFLPRAVVLAHGLAKKLEELRKSKLLSWLKPDGKTQVTMQDG
                     KIHTVLISTQHDAAVSQETIKEQLIQHLIGPAVGDISGIEILVNPTGKFELGGFEADT
                     GLTGRKIIVDTYGGVLPHGGGCFSGKDPSKVDRSGAYMARFVAKNLVAQGYAKECLVA
                     VAYAIGRAEPVMVEAINEQGESIGALAREKFDFRPQAIIERLNLRRPIFQQTAAYGHF
                     GRQGFPWEEIA"
     gene            4157..4435
                     /locus_tag="VFE94_04560"
     CDS             4157..4435
                     /locus_tag="VFE94_04560"
                     /inference="COORDINATES: protein motif:HMM:NF021402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HZX50382.1"
                     /translation="MAKWQGKKFGGTHTTLIEAAEPLVVAAENLPEVKKIVLGIIISK
                     PNKMGKERIKFTQMSGGLKATVRGAKGIQEIWIYSDFPEQVLKNLQLS"
     gene            4499..5225
                     /locus_tag="VFE94_04565"
                     /pseudo
     CDS             4499..5225
                     /locus_tag="VFE94_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(5284..6324)
                     /locus_tag="VFE94_04570"
     CDS             complement(5284..6324)
                     /locus_tag="VFE94_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50383.1"
                     /translation="MKNVARRVITVGGIRGKSFTVKSLELLARTRGYPTVAKITGDYP
                     IWVRNGEVEPIERPKGIVSLNETLAFKDFCDEDTFVVLENQGIKPETMRYFNNKVARP
                     NVALIVNIELDHTNTMGRTLERIASSTGYGIGRWPELVLSGERNPALNRIIAKRLNKH
                     QQFLPVNETREEEQAPMMDLLGLLDATLQRTGLGGLTCEEDNLLRSRIAESFTIRESS
                     SGLLYFDAAKINDLGSAKKTFTYLHARYPDKTFHFVAYFRKDRQERTEYFIPWFRELV
                     NDEAVRAMHFVGHTNRMAARHVKARVRTWKDSPTSIPFICEHAQNDASVIILACNAVN
                     PFMRAFRAHLAS"
     gene            complement(6321..6875)
                     /locus_tag="VFE94_04575"
     CDS             complement(6321..6875)
                     /locus_tag="VFE94_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HZX50384.1"
                     /translation="MVKFVRAEKAAHTTFLGEMAMCITWFPQAALELNTWIPYIVLLV
                     GFLVSTFIEEKFGYRILGVIAVPLLALYTFVQPWILLIVLIAAVVLWGVGYLLQEWMP
                     IYGRRLFRVLCGISIILTAPIFEFTGLGLDVVAFATLLPGIFAYNYLAGGPDKIRATG
                     TMMAQTAGLIAFAYGLNIIGRIIV"
     gene            7038..7400
                     /locus_tag="VFE94_04580"
     CDS             7038..7400
                     /locus_tag="VFE94_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50385.1"
                     /translation="MPKGTKMLVLILDPYDPFTLDLQASALSNPEFGIVQEMHKGSGP
                     GLEEVWDVLQEDGKVAEFLKGQVKPLLQIPEAEMQGITPSQIFFQHADKIVQRLVMSH
                     LTPVERGLLYDVFMGRVG"
     gene            7527..9020
                     /locus_tag="VFE94_04585"
     CDS             7527..9020
                     /locus_tag="VFE94_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZX50386.1"
                     /translation="MTTVRKTKFMVTYGPSCKEFRILRRLIELGADVFRFNGAHVKKA
                     NYGEIEKAIEFIRKTAAELGRVVAIFFDLGGVKFRLGSIGLDGKGKIELVEGQEVWIR
                     AIEFSDDPSVLPFPDAEVLGALVPGNFVYIADGTVRLEVQTNDERGAWARVQIGGEVS
                     SHKGVNLPGVTINRPVLTNDDMDMVAFGERLRVEWFGISYVYDGPSTKVASDYIRKTR
                     AKVMAKLERPEACGGSNLDDILEEVDAVMVARGDLGVEGPREDVPDAQEDILKRSRKK
                     TKLGMVGTQVLLSLKDEEQPSRGEVSGMVHDIRIDLAGSLLFSDEIAIGDHPELVVQE
                     ADVIARKAESRIAYYLEDLPANNKTETTAKSACMQARDYPNAVIIAITSTGRTAIAVA
                     KFLPEARILAYAHDPYTLAQLAMVGGVEPVGVLPVADFNNLVASAIRESINNGSLNPD
                     VVEHVILVLGYVPGVTDSTNATLHLDREDYLRLVATYQSQEEAICVS"
     gene            9200..9457
                     /locus_tag="VFE94_04590"
     CDS             9200..9457
                     /locus_tag="VFE94_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50387.1"
                     /translation="MWYIGEKEKTRRAFMEGIEENGVRQIIQQRFAGVSPPLEIHLSA
                     IRRDAERMVKEIPLLFTTPDEVAQVYTNVVVVDLEIPIVES"
     gene            9951..10298
                     /locus_tag="VFE94_04595"
     CDS             9951..10298
                     /locus_tag="VFE94_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HZX50388.1"
                     /translation="MSDIEKLTQRIIEFRDSRDWKQFHNPKDLAISLSLEAAEVLEHF
                     QWKNGQELEEYLKTNKGEVADELMDVLYWVLNMCSDLEIDIPEAFEKKMQKNEAKYPV
                     GTAKGKKEKYSEL"
     gene            10446..11042
                     /locus_tag="VFE94_04600"
     CDS             10446..11042
                     /locus_tag="VFE94_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VII domain-containing protein"
                     /protein_id="HZX50389.1"
                     /translation="MLSKMVDSPKRFVIAKRDSVAPEEIVVEKGDVGVIKSEAKNHAS
                     VFFVRIWKEVQLGKNDLQVIDVKKTGDSFSKKICNVCHKLKKTSEFAKNQNAKNNRSV
                     RRPTCRDCRIKMEGVAVARADRAKWLKDKPINEPFECPICHKRTIAGVTSKVVLEHDH
                     RTGKSAGWICDSCNTGLGRFKDDVELLKSAITFLKKNY"
     gene            complement(11039..11767)
                     /locus_tag="VFE94_04605"
     CDS             complement(11039..11767)
                     /locus_tag="VFE94_04605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZX50390.1"
                     /translation="MRTFPPNSIDLVVTSPPYDELRNYKGYSFDFEGMAKGLFRVMKK
                     GGIIVWVVGDKIKKGNKSLTSFKQALLFQEIGFNVHDVMVYKKRNTPFMRTNAYTNCF
                     EFMFVFSKGSPKTFTPLKSKTARQGQEMLPFNKGPDGVNKKVLGELKAEKTMTNIWEY
                     AVGLHGTTSDKIAFEHPAIFPEKLAEDHILSWTKPQDVVFDPMCGSGTTCKMAVKNER
                     FYIGCDISKEYVELTKKRLRLNNL"
     gene            complement(11882..12457)
                     /gene="lexA"
                     /locus_tag="VFE94_04610"
                     /pseudo
     CDS             complement(11882..12457)
                     /gene="lexA"
                     /locus_tag="VFE94_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
     gene            12775..13011
                     /locus_tag="VFE94_04615"
     CDS             12775..13011
                     /locus_tag="VFE94_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50391.1"
                     /translation="MNNQNKKCESCGEPLMPSQYVRIKREGEPSMRAEDALVCRNYPA
                     CEKAEKEIQSEEKRKTEWANEKKHGAEWENEQKH"
     gene            complement(13157..13576)
                     /locus_tag="VFE94_04620"
     CDS             complement(13157..13576)
                     /locus_tag="VFE94_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50392.1"
                     /translation="MAVFSLALPLERKWYKKRLKEATIQVVAEYEIKMLKSTEEVLKE
                     VDEKIQETRNHMDQRYKQVAQEVRAELARIVSHAAWIAAQRDALTEVLWKTKAKGTFK
                     EWFYNHEDSTTGEITIRITEDIVSVTSPGKEDFVVHF"
     gene            13744..13947
                     /locus_tag="VFE94_04625"
     CDS             13744..13947
                     /locus_tag="VFE94_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50393.1"
                     /translation="MDEQHILQKLEEQEKKLEAIYRSVEKTRKYFLWTLIITAVVFLL
                     PLLGLLFLIPKVLSLYATGLEGL"
     gene            14024..14278
                     /locus_tag="VFE94_04630"
     CDS             14024..14278
                     /locus_tag="VFE94_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50394.1"
                     /translation="MKPIGKVIHYYGKAGVAIVKLEDQLKVGDRIRMEKGEYVFDQEV
                     TSMQKEYKPIEQAQAGDEVGVKVGQKAPEGSLVGLLEEGE"
     gene            14307..14870
                     /locus_tag="VFE94_04635"
     CDS             14307..14870
                     /locus_tag="VFE94_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50395.1"
                     /translation="MFDLTVSAFIVIGLRLLLPLTIFKWRVFGTLVSPLVDALDVVLV
                     DVLVLLLHEPQTGFGNHYQLIDKWLDMYYFTLEVMVSFWWANKLARNTSIALYLYRFL
                     GMILFEITGIRKLFFFFPNLFENFFMYYVVAEKFFPRLVPTTKKQLAVVLLLLYIPKF
                     GQEWMLHYAQLHPWGWIRETFLPFIIR"
     gene            14928..15383
                     /locus_tag="VFE94_04640"
     CDS             14928..15383
                     /locus_tag="VFE94_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX50396.1"
                     /translation="MSKSILIILGVVVIAGVGWYLWDSGMLMQPGNEGENTMEETNEV
                     VVALAEQNGSGEMGTATLSEADGTLKVVLELTGAPAGVTQPAHIHFESCANIGGVKYP
                     LNSPVNGYSETIVDVSMAELQAGLPLSLNVHKSVEEASVYVACGNVMIP"
     gene            15465..>16433
                     /locus_tag="VFE94_04645"
     CDS             15465..>16433
                     /locus_tag="VFE94_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX50397.1"
                     /translation="MTQQEALDILKMGVHVYLTGEAGTGKTYVLDKYISWLRSHEIEP
                     AVTASTGIAATHLNGVTIHSWSGIGVRDTISASDLERMEEKRPLWSRLNNSKVLIIDE
                     ISMLSGEFFDMADQVLRHIRRTNTPFGGMQVVCSGDFFQLPPVARGSVVPAYAFESEA
                     WRQLNPVVCYLGEQYRHGGNEFLGILAAIRNRNVTEETRSLLVGRSHGELKDTDKIMR
                     LFTHNRDVDAINAEHLAKLKGKEKVFVMQQAGKGHHVELLMRGCLAPESLVLKKGAKV
                     MFVKNDHGGQYVNGTQGTVTGLKDKVAVVKTRAGRTIQAESASWKLE"
BASE COUNT         4364 a         3552 c         4252 g         4265 t
ORIGIN      
        1 agcgaattat gattcaggcc gaaaagatat aagtaacata acaaaaacat atgagtacca
       61 aagtatttta tcgagttgtt gcgtgcgtag tgagcttcgt atttttatgg ggagctacat
      121 cgtacgcgga ggcccagacg acgacaacgg gcaccggatt cgaggttgcc caaaaggtgc
      181 gaaatctcac ccagcaaaag tttgactggg tagacaaggt gcaggcttca ccacaggatc
      241 gcgtggagtt tcagattacc gtaacgtggc ggggatctgc caccacggaa aatgtgctgg
      301 tgcgggagat tcttgcagat aagctctcgt acgccggtaa cttaagggtc gatagcaacc
      361 aggtagcggg aaacgttacc acagagaata ttaatatagg aagtttaaac accaaccagg
      421 cgcgaacaat aacatttgaa acactcgtgg ttgatgcagc gtccttgccg gcgggtactt
      481 taaacctggt aaacaccgtt accgtgttta acacccaagg cggagcttct accacagccc
      541 aggtgcaggt gacaagagac ggcaagccca ccgatgtttc gaccgggcct ttgagcctgt
      601 ggcagattgg atttgcgctg atgctcttgg ttatgggtgt tgcgagcgct gcattctttg
      661 caaaatacta ctttactcgc gaagtgctgc aaagcccgta tgaaaaccgc accgatcgaa
      721 agttagcctc tatgattgaa aaaggaaagc ggcaatccta accctctgtt ttccacagcc
      781 tcgacgtatt gtcggggctg tttgctgttt gaaaagaatg gtacaataaa atatatggaa
      841 acatttgtaa aagcagacat tttctttttt gttaccaccg ttgcaatttt tttggtaaca
      901 atattgctgg ctgtcgcgtt agtttatgcg attcggatct tgcgcgacgt gcagcatatg
      961 gtgcgcaggg caaaagaaga gtccgacggg ctttttgaag atataagcca gttgcgcgcg
     1021 tttatacggg agaaagggca gaaagtgggc gccgtgcacg aaatcgtaaa gggcataggc
     1081 aaattagttt cttcacgaaa gaaaccggca aaaagcagca aaaaagcata agtaaagggt
     1141 tgtggataac ttatattgac ctcttctctc aaaagaaata taatacgttt gcgacagatt
     1201 taggaagctt ttcaaagttt cccgctgtag caaagcaccc catatatatc cacaaagtac
     1261 aaacaagaaa aggacgcctg cttcatgtag acgtcctctt cactttttat agaggattta
     1321 atacaatgaa tttgcaatga acaaaatatt ttttgccatt ttgggagtgt ttgtggtggc
     1381 ggttggagct tttgcagggt ttacgtattg gcagcagttt cggggagcag gccccgcaat
     1441 acttccgcca ggagacgaca ttggcgagat tatcgacgaa acgccgcccg aggggcagaa
     1501 caatacggat tttcctctta agcttcccga cgggttttct atttcggtat ttgccaaaga
     1561 tttgccgggc gcccgcgtta tggcgttcga cggctttggc aacatgtggg taagccagac
     1621 ttccgaaggc aaaatttcgc ttctggaagt agaagacggc aaagttacca agcagtaccc
     1681 gattttgcag gatttgcgcc gtcctcacgg gttagctttc cacccccagc agccctttac
     1741 attgtacttt gccgaggaag acagtgtttc caaaatcccg gtatattctg acgcgccctc
     1801 ccaaaagctt tttgaccttc ccgcaggagg agggcatttt acccgtacca ttctgtttgc
     1861 gcccgacggc cgcctgctca cttcggtggg ttcttcgtgc aacgtgtgcc acgaggaaga
     1921 ttggcgaaga gcttcggttt tggtttcaga cgacgagggc aaaaacctca aaacatttgc
     1981 caggggactg cgtaacgccg tgttcatggc taccaactat gtaaccggcg acgtatgggt
     2041 taccgaaatg ggccgcgaca acctgggcga cgacattcct cccgacgaaa ttaatatttt
     2101 gcaagaggaa ggcaactttg gctggcccat ttgctacgga aaaaatattc acgacaccaa
     2161 ctttgataaa aatacgtata tacgagcgcc ctgcgaagag cagtttggcg aaattccctc
     2221 gcatgtagat ttgcaggcgc attcagctcc tctgggattg gcatttgtcc cagaagaggg
     2281 ttggccagag gagtactggt acgatttact tgtagcattc catggttcgt ggaatcgttc
     2341 tgaacctacg ggatataaaa tcgcacgcat caagctcgac gcgcagggta attacgaggg
     2401 gcaagaagac ttcattaccg gctggctgca gggaaatcag gcgcttgggc gccctgtaga
     2461 tattttggtg cagcccgggg gtgtcatgta tatttccgac gacaaagccg gccttatcta
     2521 ccgcgttacc ttatagtcag cgttagggaa gggaacactc cacctgccac caggtggtgg
     2581 ttcctgtgaa cgtggtaact ccaaaggtgg caagcaggag gttgatgaat atccaagatc
     2641 catacacaat gagcaaccct accaccgtgg aaaagatgac agtcctgcct ctctggaggt
     2701 ttgacatgtt gccagcagaa gtgagcatga aaaagcctcc caccgcaaac aggaggcctg
     2761 ccagcgccag cactatggga aggccgccgt tgaacgcaga aggaaccagc aggaaggtga
     2821 tgatgttgtg caccagttca aagagagcgc acaggccgca gggaggctgg cctgggcctc
     2881 cgcaggggac gagggattgg gcgtcaaccg acaggggggc agaggcaata tacagtaggg
     2941 caaagagagg caagagcagg aagcgtggca tatttcatta aagcataaat caggaaaaat
     3001 atccctgggg aaaagttgac ggttgttttg tagaagggtt ataatctttg atatgcggtt
     3061 tataccaaac ttttacacag tagagagcgt aacgtcgggg caccccgata aagtgtgcga
     3121 ccaaatttca gacgccattg tagacgcgtg cttggagcaa gacccgcgtt cccgagtggc
     3181 catggaaaca tttggcgcgc acggcatgct tgtagtcggc ggcgagatta ccaccaacgc
     3241 aaacgtggac tacgcgcaaa ttgccagaaa tgtgtataag gaaatcggct acgacaacga
     3301 tttggaaatt ataacgcgcg tggtgcgcca gtcccccgat attgcccagg gagtagatac
     3361 cggaggagcc ggcgaccagg gcattatgta cggctttgcc acgcaggaaa cgcccgagtt
     3421 tcttccgcgc gcggtagttc tcgcgcatgg ccttgcaaaa aagctcgaag agttacgaaa
     3481 atccaagctt ctttcgtggt taaagccaga cggaaaaacc caggtaacca tgcaagacgg
     3541 aaaaattcat accgtgctca taagcaccca gcacgacgcg gcagtaagcc aagaaaccat
     3601 caaagaacag cttatccaac atcttattgg gcctgcggtt ggcgatatat cgggcatcga
     3661 aattttggta aaccccacgg ggaaatttga gctggggggt tttgaagcag atacggggct
     3721 cacgggcaga aaaataattg tggatacgta tggcggcgtt ttgccgcacg ggggcgggtg
     3781 cttttcaggc aaggatccca gtaaggtgga tcgttcgggc gcatatatgg cgcgttttgt
     3841 ggcaaaaaac ctggtggcac agggatacgc aaaagagtgc cttgtggccg tggcttacgc
     3901 aatcggcaga gcagagcccg taatggtaga agctataaac gaacagggag agtctatagg
     3961 agctttggcc cgggagaaat tcgactttcg cccccaggca attatagagc gcctcaatct
     4021 tcgaagaccc atatttcagc aaaccgcagc ttacgggcat tttggccgcc agggctttcc
     4081 atgggaagag atagcataat aaatacaaat ttaatgaaca gtattcggaa tctcaaataa
     4141 tactggaaaa atccctttgg ctaaatggca aggaaaaaaa tttggcggca cccataccac
     4201 gcttatcgag gctgcagagc ctctggtcgt ggcagccgaa aatcttcccg aggtaaaaaa
     4261 gattgtgctg ggcattatta tctccaagcc caataaaatg ggaaaagagc gcataaagtt
     4321 tacccaaatg tccggcgggt taaaagcaac cgtgcgcggc gccaagggaa ttcaagaaat
     4381 ctggatttac agcgactttc cggagcaggt actcaaaaat ctgcagctgt cctgagcgca
     4441 gtctaaaggc ggttttcttt tgcatgagaa attttcaaaa aatggtacaa taaacctcat
     4501 ggaagaaatt ctcttactgt gtttgctggt gctggtggct tccgcaatcg gaacattttc
     4561 cgggtttggg ggatctacca ttatggtgcc catattgctg tttttctttc ctttgcctgt
     4621 aacgcttctt ttctcggcca ttatccattt ggttggcgac gcctggaaaa tgctgctgtt
     4681 tcgtaaggga gctcgaaaat ggaacctaat tttatggttt gcagttccgg gagccgctgc
     4741 cagcatagct agggcttttg tgtctgtcca agccccgcag gaagttttac tgcgcgtgct
     4801 gggagcgttt ttgttggcgt acgttgcgtt tttgttttta aaaccaaact ggaaactgcc
     4861 ccaaaaaaat tctaccgcgg cccttggggg gagcggcctc cggagtggct gcgggcattt
     4921 ttggcgtggg cggggctatt cgagcggcag tgctggccgc gtttaacctt cccaaagaag
     4981 tgtatttgtt tacttcggga tttaccgcgc tgtttattga cagttcacgc attgcagctt
     5041 actttgcggg cggcacgcgc ctggaaggtt tgtttttgtg ggggttattg ttatttgtgc
     5101 ctttgtcatt tttgggcgcc tttgtcgcca aaaaatttat cgaccgcgtt cccgagcaat
     5161 attttcgttt tgttattgcc ttgttcctcg gtctcgccgg cctcaaattt gttgtgtttc
     5221 cctaaactat aaataataaa aatgaacccc cgtcttgttg tacaagtacg ggggctgttt
     5281 tggttaagaa gcgagatgcg ctctgaacgc gcgcataaac gggttcactg cgttgcacgc
     5341 gagaatgatc acgcttgcgt cgttctgcgc atgttcgcag ataaaaggaa tagaagtcgg
     5401 agaatccttc catgtgcgga ctcttgcttt aacgtgtcgg gccgccatcc tattggtgtg
     5461 gccaacaaag tgcatagccc gaactgcttc gtcgttgact agttcccgga accagggaat
     5521 gaaatactcc gtccgttcct gacggtcctt ccggaagtag gcaacgaaat gaaatgtctt
     5581 gtctggatac ctggcgtgta agtaggtaaa ggtttttttc gccgagccaa gatcgtttat
     5641 ttttgccgca tcgaaataca gaagcccgct ggagctttcc cttattgtga atgactctgc
     5701 aattcgtgag cggaggaggt tgtcttcctc gcaagtaagc cccccaagtc cggtgcgctg
     5761 cagggtcgcg tcgagcaagc caagcagatc catcatagga gcctgttctt cctcccttgt
     5821 ttcattaacg ggaaggaatt gctggtgttt gtttagtcgt ttggcaataa tcctgttcaa
     5881 cgcgggattt ctttcgcccg atagcaccag ctctggccat ctcccgatgc catatcctgt
     5941 tgaactggcg attctctcta aggtacgccc catcgtattc gtgtggtcga gctctatgtt
     6001 aacgatgagc gcgacgttcg gccttgccac cttgttgttg aaatacctca tggtttcggg
     6061 cttgattccc tggttttcca ggacgacaaa ggtgtcctcg tcgcagaaat ctttgaaggc
     6121 aagagtttcg tttagcgaaa ctatgccctt tgggcgttca ataggttcta cttctccgtt
     6181 acgtacccat ataggatagt ctccggtgat tttagcgacg gtgggatacc ctcgtgttcg
     6241 tgctagcaac tcaagcgact tcaccgtaaa agattttcct cttatccctc ctacggtgat
     6301 tacccgtctc gcgacgtttt tcatactatt atcctcccta ttatattcaa gccataggcg
     6361 aacgcgatta agccggctgt ttgtgccatc atcgtgccgg tagcccgtat cttgtcgggg
     6421 ccgccagcaa ggtagttata ggcgaatatc cccggtagaa gggtggcgaa agccaccacg
     6481 tccagaccca ggcctgtaaa ctcaaagatg ggagcggtga gtataatgct tataccgcat
     6541 aaaacgcgga acagccgacg gccatatata ggcatccact cctgcaaaag ataacctact
     6601 ccccacaaca cgacagctgc aatcagcacg atgagcaaaa tccagggttg cacaaaagtg
     6661 tatagggcaa gcaatgggac tgctattacc ccgagaatcc gatacccgaa cttctcctct
     6721 ataaaggtgc tcacaaggaa tccaacaaga agaactatgt agggaatcca ggtattgagc
     6781 tccagggccg cttggggaaa ccaggtaatg cacatagcca tctctcctaa aaatgtagta
     6841 tgagctgcct tttcggcgcg aacaaacttt accattttat gggcgagtag tcaagaaagc
     6901 ttggactttt ccgctttgtt gctgccctgt ttctcgccct agccggcctt aagtttttgt
     6961 ttttcccttg aaaactgccg aaaaatgtgg tattgcaaaa gcagcaagaa ttttgtccac
     7021 gcgaattggg aggcgaaatg ccaaaaggca cgaagatgct ggttcttatt ttggatcctt
     7081 acgatccttt tactctcgac ttgcaggcgt ctgctctaag caatccggag tttggtattg
     7141 tccaggagat gcataaaggt tcaggccctg gcttagagga ggtctgggac gtcctacagg
     7201 aagacggtaa agtcgcagag tttctgaagg ggcaggtaaa gccgcttttg cagattccag
     7261 aagcggaaat gcaaggaata actccttcac aaatcttctt tcagcacgca gataaaatcg
     7321 tacagagact ggtaatgtct catttgacac cagtagagcg tggcttacta tacgatgtct
     7381 ttatgggccg agttggttaa tcatacaagc gatgcaaaag ctgcgtcgtt ttctttttga
     7441 agagcttgac attactgcat tttgaactag tctggggcta gaggagcact caatatcata
     7501 tatactgaaa gttagaggag gcagttatga ctacggtacg caaaaccaaa tttatggtaa
     7561 cctacggtcc ttcatgcaag gagtttagga tcttacggcg tcttattgag cttggggcag
     7621 atgtgtttcg gttcaatggc gcacatgtta agaaagcgaa ctacggtgag atcgagaagg
     7681 caattgagtt tattcgtaag actgctgctg aactcggtag ggttgtggcc atcttttttg
     7741 acttaggcgg cgtaaagttt cgccttggca gtatcgggct tgatggtaaa ggtaagatag
     7801 agcttgtgga gggacaagag gtttggatcc gagctatcga gttttctgat gatccgagcg
     7861 tgttgccgtt tcccgatgca gaagtattgg gagctctcgt acctgggaac tttgtgtaca
     7921 ttgccgatgg gacggtgcgc cttgaagttc aaacgaacga cgaaagagga gcctgggcgc
     7981 gcgtacagat cgggggagag gtttcttctc ataaaggagt caatctgccc ggtgtcacta
     8041 ttaaccgacc tgtcttaacg aacgacgaca tggatatggt agcgttcggc gagcgcctgc
     8101 gcgttgaatg gtttggtatt tcttatgtgt acgatgggcc aagcacaaaa gttgcctccg
     8161 attatatcag gaaaacaaga gccaaggtga tggccaagct ggagcggccg gaggcctgcg
     8221 gggggagcaa tctcgacgat atcctcgaag aggtagatgc ggttatggtc gcccgaggcg
     8281 acctgggtgt tgaaggcccg cgagaggatg taccagatgc tcaggaagat attctgaaaa
     8341 gatcgcggaa aaagactaaa cttggcatgg ttggaactca ggttttgctg tctctgaagg
     8401 acgaagagca gcccagtcgt ggggaagtat cgggaatggt acacgatatt aggattgatc
     8461 tggcaggcag cctgctgttc tccgatgaga ttgcgatagg tgatcatccg gagctcgtcg
     8521 tacaggaagc agatgtgatt gcgcgtaagg ccgaaagtcg catcgcttat tatttggagg
     8581 atctcccggc aaacaacaag accgagacta cggccaagtc tgcatgtatg caggcgcgcg
     8641 attaccccaa tgcggtcatt attgctataa cgtctaccgg gcgtacggca atagcagtcg
     8701 caaagttcct gccggaagcc cgtattcttg cgtatgccca cgacccttat acgttggccc
     8761 aactcgcaat ggtaggtgga gtggagccgg ttggggttct gcctgttgcc gactttaaca
     8821 acctagtagc ttcggcaata cgagagtcga taaacaatgg ttctttgaat ccagatgtgg
     8881 tcgagcacgt tatactcgta cttggctatg tgcccggcgt caccgacagc acaaacgcta
     8941 cgctacacct ggatagggaa gactacctaa gattggtagc tacatatcaa agccaggagg
     9001 aggctatttg cgtttcatga agtaaagcac gtatcggggc ggggcagaga aattctgcgc
     9061 cgcccaattt tttggttaaa atgggaaacg tggtgattat tctggatcct tctgctctgg
     9121 atcccagaaa gcctggcaaa aagccagggt tcggcagcac cccgccgagc cttttctttt
     9181 tacttgaaaa atctcgaaaa tgtggtatat aggagaaaag gaaaagacaa ggagggcatt
     9241 tatggaagga atagaagaaa acggtgtgcg gcaaatcata caacagcgtt ttgcgggagt
     9301 atctccccct ctagaaattc atctctccgc aatcagaaga gatgcggaga ggatggtgaa
     9361 agaaataccg cttctcttca ccacgcccga cgaagtagcc caggtctaca cgaacgtggt
     9421 ggtcgttgat ctggagatac cgatcgtaga atcgtagatc agtagcctaa cgatatcgca
     9481 tctgcgcgat accgctaaaa ctttaggtca ccaacggaaa ccgtggtgac ctcttttttt
     9541 actggaaaaa tcctgggaag tgtggtatta cagttgtagt attctataga tatatatgtt
     9601 gatgggggag taatggatgt gctcggatgg tttgagagga tggtttggta taccttcctc
     9661 tcggtaatgg ttttgatggg cagttttata ctcaaatccc gccacgagga cccaaatatc
     9721 ctggcattgg gtgtactcgc tgcgtttgct ttctttttcc cgtcggttgt gcttgcgacc
     9781 ttggattgca tcataaaacc cctatttgat gcggaagagg acgacgatta aggctgtgcc
     9841 ctggggtaca gtcttctttc ttacgccagt cctcaccgtg tcctttagaa agtttccaaa
     9901 aaggggggaa agttcttgaa caaacgagga aaatatggca tggtagggta atgtcagata
     9961 tcgagaagct tacccagcgt attatagaat tccgcgacag ccgcgactgg aagcagttcc
    10021 acaacccaaa agacttggcc atatctttgt ctttggaagc agccgaagtg ctggagcact
    10081 tccagtggaa aaatgggcaa gagcttgaag aatacctaaa aacaaataag ggcgaggtgg
    10141 cagatgagtt aatggatgtg ctctattggg tgctcaatat gtgcagtgat ttggaaattg
    10201 atattcccga agcctttgag aagaaaatgc aaaaaaacga ggcaaaatat cccgtcggca
    10261 ccgccaaagg caaaaaagaa aaatactccg agctttaagc aaaacctcgc catttcggcg
    10321 attttttgtt tgggttgaaa caagcccatc gtgtcccgcc gaagctcttg agtaaacgcg
    10381 gaaaatatga catggtaagg tattataaaa ggcgtatcca aacatatgaa taaaggcctg
    10441 ctaagatgct ttctaaaatg gtagactcac ccaaacgatt cgtgatagca aaaagagaca
    10501 gtgtagcccc tgaagaaatt gtggttgaaa agggcgatgt tggtgtcata aagtcagagg
    10561 ctaaaaatca cgcctcggta ttttttgtaa gaatttggaa agaagttcaa cttgggaaaa
    10621 atgacctcca agttatagat gttaaaaaga cgggtgacag tttttcaaag aaaatttgta
    10681 atgtctgcca caaactcaaa aaaacttcgg agtttgccaa gaatcagaat gcaaagaata
    10741 atagatccgt aagaaggcca acatgcaggg attgtcgaat aaaaatggaa ggagtcgcgg
    10801 tagctcgagc ggatagagcc aaatggctaa aagataaacc gataaacgag ccttttgaat
    10861 gtcctatttg ccataagaga accatcgctg gcgtaacaag caaagtagtt ctcgagcatg
    10921 atcaccgtac gggaaaatct gccggttgga tttgtgatag ttgtaatacg ggattgggtc
    10981 gttttaaaga cgatgtggag ctacttaaaa gtgcgattac tttcctcaag aaaaactatt
    11041 aaagattatt gagcctcaat cttttttttg taagctctac gtactcttta gatatatcgc
    11101 agccaatata aaaacgctca ttctttactg ccattttgca ggttgtccca gagccgcaca
    11161 tcggatcgaa gaccacatct tgtggtttag tccatgagag gatatgatct tcagctaatt
    11221 tctcaggaaa gatcgcagga tgttcgaaag ctatcttgtc gcttgttgtg ccatgcaaac
    11281 ctacagcata ttcccaaata ttcgtcatgg tcttttcggc tttaagctcg cccagaacct
    11341 ttttgttgac tccatctggc cctttgttaa agggcaacat ttcttgtcct tgtcttgcgg
    11401 tcttactttt taaaggagta aatgttttgg gagagccttt actgaaaaca aacatgaact
    11461 caaaacagtt tgtatacgca ttcgtcctca taaaaggcgt atttcttttc ttgtaaacca
    11521 tgacatcatg aacattaaac ccgatttcct gaaagagaag agcctgttta aaacttgtaa
    11581 gacttttatt ccctttttta attttatccc caacaaccca aactataata cctccttttt
    11641 tcatcactct aaataaacct ttagccatac cttcgaagtc aaaactataa cctttgtaat
    11701 ttcgcagttc gtcataagga ggtgaagtta ccacgagatc tattgagtta ggtggaaatg
    11761 ttctcattac ctcgatagca tctccacaaa agactttatt tagtggtaat tcttcatact
    11821 ctcccactct tttatattct gggagttgaa tcgttgaagc tccttcttca tgaatgacat
    11881 ctaatactat accctgtata gcaagttctc gatttttttt gataataatt ggttcgatcc
    11941 tcttatttgc tggttgcaag cgaatatatc ctcgttcttt gtaaaaagtt tttaaggttg
    12001 tttcctggtt atcgataaga gccacaacct tttggccatt ttctgcgact ccttgttgtt
    12061 ttaccaaaac tatgtctcca ctattgatat tttcgtctat catgctgttg ccaaccacgc
    12121 ggagggcgta aacatcccca aattttggaa gttttgtttt tggaactgct atatattctt
    12181 tattttcgat tgcttcgatc ggttcaccag ccgcaatagt gcccaagaga ggaatacgca
    12241 ccatttgctc tcggttgttt atatcgattg cgcgtatgcg attggcggtt cttttaaggt
    12301 aacccttatc ctctagggct tttaggtgtt ggtgtacggt tgccttggag gaaaggcgaa
    12361 agtgcttttt gatctcgtct aaagagggag cgtagtcgtg cttttgaatg tacccttgga
    12421 tatattcaaa aatttgtttt tggcgttttg taagcatagt ggtaggttgg ggaaaagtgg
    12481 ggacttgcaa agttcggtgt tcgttcggta taatatcatt gtatcgcacc aagcgcacga
    12541 gtcaatgtgg aaaactaaaa aggttagata atcaaattaa ataaaaaggc aaaacaaaaa
    12601 atatgccgtg gaaactaatc aaagatccaa agggaccact agtccctcca aaactaccac
    12661 gaatgcccag tttgcctcgt gctccccggg tgccaaaact gcccagtcta ccaaaacccc
    12721 caaaaccgcc cgcccctcct ggttataagt atatttggcg agataaatag agctatgaac
    12781 aatcaaaaca aaaaatgtga aagctgtgga gagccactaa tgccttcaca gtacgtacgt
    12841 ataaaaagag aaggcgaacc atcgatgagg gcagaagatg ctcttgtctg ccgaaattat
    12901 ccggcctgcg aaaaggcaga gaaagaaatc cagagtgaag aaaaacgtaa aacagaatgg
    12961 gcgaatgaga agaagcacgg agcagaatgg gagaacgaac agaaacatta gtattctaaa
    13021 aatctatgaa aaaggaggga atgtatggaa tatttgtcgg ttgagggatt ttttagtttt
    13081 ttggtagaat aaatctataa aaatgaagaa gcggcacgca tacggtgccg ccttggctgt
    13141 ttgagggagc tgttggctag aagtgtacta caaaatcttc tttgccagga gaggtaacag
    13201 atactatgtc ttcggttatc cgtatcgtta tctcgcccgt tgtgctgtct tcgtggttgt
    13261 aaaaccattc tttgaaagtt ccttttgcct tggtcttcca aagaacttct gttagtgcat
    13321 ctcgctgcgc cgcaatccat gcggcatggg atacaattct tgccagttca gcccttacct
    13381 cttgtgccac ctgtttatac cgctggtcca tgtgattgcg agtctcttgt atcttttcgt
    13441 caacctcctt aagaacttct tctgtagact ttagcatttt tatttcgtat tcggcgacaa
    13501 cctgtattgt tgcctccttc agccttttct tgtaccactt tctctctaga ggcaaggcca
    13561 gggagaagac cgccatacaa accattactg tcattagtac cacggagaag gtgctcatag
    13621 taggtgctcc tttcagcaaa gatgtggtaa gattatagcc taaatttcat agcttggcaa
    13681 ggttgatggc atccaatcaa gaaggttttt gcttgcgcga ggataaaggt ttacaataaa
    13741 ggtatggacg aacaacacat attacaaaag ttagaagagc aggagaaaaa gctcgaggct
    13801 atttaccggt cggtggagaa gacaagaaag tattttctgt ggacgttgat tattacggcg
    13861 gtagtgttct tgctgcctct tctgggcctt ttgtttctta tccctaaagt gttaagcctg
    13921 tacgccaccg gcttggaagg gctgtagctt gccgtggagt ttttgtaggg gtagtatgga
    13981 agcataaggg gaaaggtcgc aaactgggtt tcaactacat attatgaaac caataggcaa
    14041 agttattcac tactacggca aggcgggggt agcgattgta aaactcgaag accagctcaa
    14101 ggtgggcgac cgcatccgca tggaaaaagg agagtatgtg ttcgaccagg aagttacttc
    14161 tatgcaaaaa gagtataagc ccattgagca ggcccaggcg ggcgacgagg tgggggtaaa
    14221 ggttggccaa aaagcgcccg aaggatctct tgtgggcttg ctggaagaag gagaataata
    14281 gggggctgga cgagaattgg aatacggtgt tcgaccttac ggtatctgca tttattgtaa
    14341 tcgggctgcg cttgctgcta cccttaacca tttttaaatg gcgggttttt ggaactctgg
    14401 tttcgccttt ggtggacgcc ctagacgttg ttcttgtcga tgttttggtg ctactgctgc
    14461 acgagcccca gaccgggttt ggcaaccatt accaacttat agataagtgg ctcgacatgt
    14521 attactttac cctcgaggtt atggtgtctt tttggtgggc caacaagctt gccaggaata
    14581 ccagcattgc gctgtacctg taccggtttt taggcatgat tttatttgaa attaccggca
    14641 tacgaaaact gtttttcttt tttccaaact tatttgaaaa cttctttatg tattatgtag
    14701 ttgcagagaa gtttttcccg aggctggtgc caacaacaaa aaagcagctt gcggttgttt
    14761 tgctgctgct gtacattcca aagtttggcc aggagtggat gctgcactac gcgcagctgc
    14821 acccgtgggg gtggatccgc gaaacctttt tgccctttat aatccgttaa ggtacaatag
    14881 ccataggagc gcaaaggtcg ttttctacct aacaagaaaa tttcgttatg agcaaatcga
    14941 ttcttattat tttgggagtt gttgttatcg ccggagtggg ttggtatctg tgggattcgg
    15001 gaatgttgat gcagccaggc aacgagggcg agaatacgat ggaagaaacc aacgaagtag
    15061 tggtagccct tgcagagcag aatggctctg gggagatggg aaccgccacc ctgtctgaag
    15121 cagacggaac gctcaaggtt gtattggaat taaccggcgc accagcaggc gtaacgcagc
    15181 ctgcccatat ccattttgag tcgtgcgcga atatcggcgg agtaaagtat cctttgaact
    15241 ccccggtaaa cgggtactct gaaaccatag tagacgtttc tatggcagaa ctgcaggccg
    15301 gattgccttt gtccctgaac gtgcacaagt cagtagagga ggcaagcgtg tacgttgcgt
    15361 gcgggaatgt catgataccc tagttgtgtc gtacagcaga gtgaaagaca aagtcgccct
    15421 tcgccgtctg gcggattggc ggctttttga tacactaaag cagtatgacg cagcaagaag
    15481 cgctggatat tttaaaaatg ggagtacatg tgtaccttac gggggaggcg ggtaccggca
    15541 aaacctatgt gctcgataaa tatatttcgt ggctgcgcag ccacgaaata gagcctgcgg
    15601 taacggcatc taccggcatt gcggcaactc acttgaacgg cgttaccatt cattcgtggt
    15661 ctggcattgg cgtgcgagat accataagcg cctctgattt ggagcggatg gaagaaaaaa
    15721 ggccgctctg gagcaggcta aacaattcca aggtgttaat catcgacgaa atctccatgc
    15781 tttccggtga gttttttgat atggcagacc aggttctgcg gcacataagg agaaccaata
    15841 cgccgtttgg gggcatgcaa gtggtatgct ccggggattt tttccaactg ccgcctgtag
    15901 cgcggggaag cgttgtgcct gcttacgcgt ttgagtctga agcgtggcgg cagctcaacc
    15961 ccgtagtatg ttatcttggc gagcaatatc ggcatggcgg caacgagttt ttgggcatat
    16021 tggctgctat tcgcaaccga aacgttacag aagaaacgcg cagcctgctt gttgggcgaa
    16081 gtcatggcga gctcaaagat acagacaaga ttatgcggct ctttacccat aacagggatg
    16141 tcgacgcaat aaatgcagaa catttggcaa agctcaaggg aaaagaaaaa gtgtttgtca
    16201 tgcagcaggc aggcaagggg caccatgtgg agctcttaat gcgcggctgc ctggcgccgg
    16261 agtctttggt gctcaagaaa ggggccaaag taatgtttgt aaaaaatgat cacgggggac
    16321 agtatgtaaa cggcacgcaa ggaacagtaa cggggttaaa ggataaggtg gctgtggtaa
    16381 aaacgcgtgc aggcagaacc atacaggcgg agtctgcgag ctggaaattg gaa
//
